CA2580548A1 - Surveillance dynamique de l'adhesion et de la diffusion cellulaire a l'aide du systeme rt-ces - Google Patents
Surveillance dynamique de l'adhesion et de la diffusion cellulaire a l'aide du systeme rt-ces Download PDFInfo
- Publication number
- CA2580548A1 CA2580548A1 CA002580548A CA2580548A CA2580548A1 CA 2580548 A1 CA2580548 A1 CA 2580548A1 CA 002580548 A CA002580548 A CA 002580548A CA 2580548 A CA2580548 A CA 2580548A CA 2580548 A1 CA2580548 A1 CA 2580548A1
- Authority
- CA
- Canada
- Prior art keywords
- cell
- impedance
- cells
- electrode
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000007480 spreading Effects 0.000 title claims abstract description 114
- 238000003892 spreading Methods 0.000 title claims abstract description 114
- 238000012544 monitoring process Methods 0.000 title claims abstract description 85
- 230000021164 cell adhesion Effects 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 169
- 150000002894 organic compounds Chemical class 0.000 claims abstract description 63
- 238000000576 coating method Methods 0.000 claims abstract description 26
- 239000011248 coating agent Substances 0.000 claims abstract description 25
- 238000004377 microelectronic Methods 0.000 claims abstract description 23
- 210000004027 cell Anatomy 0.000 claims description 1154
- 150000001875 compounds Chemical class 0.000 claims description 343
- 238000012360 testing method Methods 0.000 claims description 244
- 239000000758 substrate Substances 0.000 claims description 206
- 238000003491 array Methods 0.000 claims description 141
- 230000008859 change Effects 0.000 claims description 128
- 238000002847 impedance measurement Methods 0.000 claims description 89
- 238000005259 measurement Methods 0.000 claims description 48
- 239000000243 solution Substances 0.000 claims description 38
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 claims description 30
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 claims description 30
- 238000009739 binding Methods 0.000 claims description 28
- 230000027455 binding Effects 0.000 claims description 26
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- 102000004169 proteins and genes Human genes 0.000 claims description 22
- 210000002744 extracellular matrix Anatomy 0.000 claims description 19
- 102000005962 receptors Human genes 0.000 claims description 19
- 108020003175 receptors Proteins 0.000 claims description 19
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 17
- 102000006495 integrins Human genes 0.000 claims description 16
- 108010044426 integrins Proteins 0.000 claims description 16
- 239000003446 ligand Substances 0.000 claims description 14
- IYMAXBFPHPZYIK-BQBZGAKWSA-N Arg-Gly-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(O)=O IYMAXBFPHPZYIK-BQBZGAKWSA-N 0.000 claims description 13
- 239000003112 inhibitor Substances 0.000 claims description 13
- 230000001788 irregular Effects 0.000 claims description 13
- 239000000523 sample Substances 0.000 claims description 13
- 108010001857 Cell Surface Receptors Proteins 0.000 claims description 11
- 230000001965 increasing effect Effects 0.000 claims description 11
- 239000012811 non-conductive material Substances 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 230000001413 cellular effect Effects 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 210000005260 human cell Anatomy 0.000 claims description 7
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 239000000556 agonist Substances 0.000 claims description 5
- 108020004414 DNA Proteins 0.000 claims description 4
- 239000005557 antagonist Substances 0.000 claims description 4
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 4
- 239000013068 control sample Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 4
- 239000012085 test solution Substances 0.000 claims description 4
- 102000053602 DNA Human genes 0.000 claims description 3
- 210000002865 immune cell Anatomy 0.000 claims description 3
- 239000010980 sapphire Substances 0.000 claims description 3
- 229910052594 sapphire Inorganic materials 0.000 claims description 3
- 229910052710 silicon Inorganic materials 0.000 claims description 3
- 239000010703 silicon Substances 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 235000012239 silicon dioxide Nutrition 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 2
- 230000006854 communication Effects 0.000 claims description 2
- 230000002829 reductive effect Effects 0.000 claims description 2
- 230000008093 supporting effect Effects 0.000 claims description 2
- 102000006240 membrane receptors Human genes 0.000 claims 3
- 230000012292 cell migration Effects 0.000 claims 1
- 230000017455 cell-cell adhesion Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 description 135
- 239000012530 fluid Substances 0.000 description 93
- 230000004044 response Effects 0.000 description 56
- 230000000694 effects Effects 0.000 description 55
- 230000012010 growth Effects 0.000 description 45
- 102000016359 Fibronectins Human genes 0.000 description 42
- 108010067306 Fibronectins Proteins 0.000 description 42
- 230000036962 time dependent Effects 0.000 description 41
- 238000000423 cell based assay Methods 0.000 description 30
- 230000003013 cytotoxicity Effects 0.000 description 30
- 231100000135 cytotoxicity Toxicity 0.000 description 30
- SCJNCDSAIRBRIA-DOFZRALJSA-N arachidonyl-2'-chloroethylamide Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(=O)NCCCl SCJNCDSAIRBRIA-DOFZRALJSA-N 0.000 description 28
- 230000036755 cellular response Effects 0.000 description 24
- 230000001404 mediated effect Effects 0.000 description 22
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 22
- 239000000725 suspension Substances 0.000 description 20
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 19
- 239000002953 phosphate buffered saline Substances 0.000 description 19
- 210000002325 somatostatin-secreting cell Anatomy 0.000 description 19
- 230000010261 cell growth Effects 0.000 description 18
- 230000006399 behavior Effects 0.000 description 17
- 238000004364 calculation method Methods 0.000 description 16
- 230000006907 apoptotic process Effects 0.000 description 15
- 230000004663 cell proliferation Effects 0.000 description 15
- 238000001514 detection method Methods 0.000 description 15
- 230000007402 cytotoxic response Effects 0.000 description 14
- 210000004292 cytoskeleton Anatomy 0.000 description 13
- 231100000433 cytotoxic Toxicity 0.000 description 13
- 230000001472 cytotoxic effect Effects 0.000 description 13
- 238000012806 monitoring device Methods 0.000 description 13
- 238000011002 quantification Methods 0.000 description 13
- 210000001519 tissue Anatomy 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 12
- 239000006285 cell suspension Substances 0.000 description 12
- 230000006870 function Effects 0.000 description 12
- 102000004196 processed proteins & peptides Human genes 0.000 description 11
- 239000012088 reference solution Substances 0.000 description 11
- 238000013459 approach Methods 0.000 description 10
- 238000001516 cell proliferation assay Methods 0.000 description 10
- 230000001419 dependent effect Effects 0.000 description 10
- 238000013461 design Methods 0.000 description 10
- 230000002900 effect on cell Effects 0.000 description 10
- 230000003993 interaction Effects 0.000 description 10
- 239000002245 particle Substances 0.000 description 10
- 230000030833 cell death Effects 0.000 description 9
- 231100000673 dose–response relationship Toxicity 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 230000014509 gene expression Effects 0.000 description 9
- 239000002924 silencing RNA Substances 0.000 description 9
- 102000000844 Cell Surface Receptors Human genes 0.000 description 8
- 108020004459 Small interfering RNA Proteins 0.000 description 8
- 230000020411 cell activation Effects 0.000 description 8
- 230000024245 cell differentiation Effects 0.000 description 8
- 238000009795 derivation Methods 0.000 description 8
- 239000010408 film Substances 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 230000007246 mechanism Effects 0.000 description 8
- 239000000203 mixture Substances 0.000 description 8
- 230000017074 necrotic cell death Effects 0.000 description 8
- 230000004962 physiological condition Effects 0.000 description 8
- 230000000638 stimulation Effects 0.000 description 8
- 201000011510 cancer Diseases 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000003472 neutralizing effect Effects 0.000 description 7
- 230000035755 proliferation Effects 0.000 description 7
- 238000003908 quality control method Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- -1 urine Substances 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- 102000007469 Actins Human genes 0.000 description 6
- 108010085238 Actins Proteins 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 108060006706 SRC Proteins 0.000 description 6
- 238000002784 cytotoxicity assay Methods 0.000 description 6
- 231100000263 cytotoxicity test Toxicity 0.000 description 6
- 230000004069 differentiation Effects 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 229910052751 metal Inorganic materials 0.000 description 6
- 239000002184 metal Substances 0.000 description 6
- 206010028980 Neoplasm Diseases 0.000 description 5
- 102000001332 SRC Human genes 0.000 description 5
- 230000008901 benefit Effects 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 108010045325 cyclic arginine-glycine-aspartic acid peptide Proteins 0.000 description 5
- 230000008520 organization Effects 0.000 description 5
- 230000036285 pathological change Effects 0.000 description 5
- 239000004033 plastic Substances 0.000 description 5
- 229920003023 plastic Polymers 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- 238000001228 spectrum Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 231100000699 Bacterial toxin Toxicity 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000003618 borate buffered saline Substances 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 238000003352 cell adhesion assay Methods 0.000 description 4
- 239000006143 cell culture medium Substances 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 230000002508 compound effect Effects 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 230000005684 electric field Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 210000004379 membrane Anatomy 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000010899 nucleation Methods 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 108700038288 rhodamine-phalloidin Proteins 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 101000781681 Protobothrops flavoviridis Disintegrin triflavin Proteins 0.000 description 3
- 238000003782 apoptosis assay Methods 0.000 description 3
- 239000000688 bacterial toxin Substances 0.000 description 3
- 239000013060 biological fluid Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 230000033077 cellular process Effects 0.000 description 3
- 239000000919 ceramic Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000003102 growth factor Substances 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 102000009076 src-Family Kinases Human genes 0.000 description 3
- 108010087686 src-Family Kinases Proteins 0.000 description 3
- 238000001890 transfection Methods 0.000 description 3
- FADQCEBBTITJBI-UHFFFAOYSA-N 2-[(2-methoxyphenyl)methoxymethyl]oxirane Chemical compound COC1=CC=CC=C1COCC1OC1 FADQCEBBTITJBI-UHFFFAOYSA-N 0.000 description 2
- 102000000905 Cadherin Human genes 0.000 description 2
- 108050007957 Cadherin Proteins 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 108060003393 Granulin Proteins 0.000 description 2
- 108010038807 Oligopeptides Proteins 0.000 description 2
- 102000015636 Oligopeptides Human genes 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- KPKZJLCSROULON-QKGLWVMZSA-N Phalloidin Chemical compound N1C(=O)[C@@H]([C@@H](O)C)NC(=O)[C@H](C)NC(=O)[C@H](C[C@@](C)(O)CO)NC(=O)[C@H](C2)NC(=O)[C@H](C)NC(=O)[C@@H]3C[C@H](O)CN3C(=O)[C@@H]1CSC1=C2C2=CC=CC=C2N1 KPKZJLCSROULON-QKGLWVMZSA-N 0.000 description 2
- 229940122924 Src inhibitor Drugs 0.000 description 2
- 238000005411 Van der Waals force Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 108010072041 arginyl-glycyl-aspartic acid Proteins 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 238000010822 cell death assay Methods 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 231100000317 environmental toxin Toxicity 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000011152 fibreglass Substances 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000009650 gentamicin protection assay Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 150000002605 large molecules Chemical class 0.000 description 2
- 238000000670 ligand binding assay Methods 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000010534 mechanism of action Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009149 molecular binding Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 2
- 229920001721 polyimide Polymers 0.000 description 2
- 230000003389 potentiating effect Effects 0.000 description 2
- 108091006024 signal transducing proteins Proteins 0.000 description 2
- 102000034285 signal transducing proteins Human genes 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 150000003431 steroids Chemical class 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- HBAQYPYDRFILMT-UHFFFAOYSA-N 8-[3-(1-cyclopropylpyrazol-4-yl)-1H-pyrazolo[4,3-d]pyrimidin-5-yl]-3-methyl-3,8-diazabicyclo[3.2.1]octan-2-one Chemical class C1(CC1)N1N=CC(=C1)C1=NNC2=C1N=C(N=C2)N1C2C(N(CC1CC2)C)=O HBAQYPYDRFILMT-UHFFFAOYSA-N 0.000 description 1
- 241001415166 Alona Species 0.000 description 1
- 241000182988 Assa Species 0.000 description 1
- 235000009581 Balanites aegyptiaca Nutrition 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 102100025929 Neuronal migration protein doublecortin Human genes 0.000 description 1
- 101710105341 Neuronal migration protein doublecortin Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 description 1
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 description 1
- 108010009711 Phalloidine Proteins 0.000 description 1
- 239000004642 Polyimide Substances 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000206607 Porphyra umbilicalis Species 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 229920001807 Urea-formaldehyde Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000003364 biologic glue Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000009087 cell motility Effects 0.000 description 1
- 230000008614 cellular interaction Effects 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 239000013000 chemical inhibitor Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 239000012611 container material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000003436 cytoskeletal effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000003828 downregulation Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000004870 electrical engineering Methods 0.000 description 1
- 239000007772 electrode material Substances 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000003366 endpoint assay Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000003209 gene knockout Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 108010034892 glycyl-arginyl-glycyl-aspartyl-serine Proteins 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 238000010820 immunofluorescence microscopy Methods 0.000 description 1
- 238000001453 impedance spectrum Methods 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000031146 intracellular signal transduction Effects 0.000 description 1
- 229940043355 kinase inhibitor Drugs 0.000 description 1
- 239000005355 lead glass Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 102000037979 non-receptor tyrosine kinases Human genes 0.000 description 1
- 108091008046 non-receptor tyrosine kinases Proteins 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000004789 organ system Anatomy 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 239000003757 phosphotransferase inhibitor Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 229920002714 polyornithine Polymers 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 238000011533 pre-incubation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 229910052703 rhodium Inorganic materials 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000004017 serum-free culture medium Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 210000003518 stress fiber Anatomy 0.000 description 1
- 238000004381 surface treatment Methods 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 210000000605 viral structure Anatomy 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/02—Analysing fluids
- G01N29/036—Analysing fluids by measuring frequency or resonance of acoustic waves
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2291/00—Indexing codes associated with group G01N29/00
- G01N2291/02—Indexing codes associated with the analysed material
- G01N2291/025—Change of phase or condition
- G01N2291/0256—Adsorption, desorption, surface mass change, e.g. on biosensors
- G01N2291/0257—Adsorption, desorption, surface mass change, e.g. on biosensors with a layer containing at least one organic compound
Landscapes
- Physics & Mathematics (AREA)
- Acoustics & Sound (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (47)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US61374904P | 2004-09-27 | 2004-09-27 | |
US61387204P | 2004-09-27 | 2004-09-27 | |
US60/613,872 | 2004-09-27 | ||
US60/613,749 | 2004-09-27 | ||
US61460104P | 2004-09-29 | 2004-09-29 | |
US60/614,601 | 2004-09-29 | ||
US10/987,732 US7192752B2 (en) | 2002-12-20 | 2004-11-12 | Real time electronic cell sensing systems and applications for cell-based assays |
PCT/US2004/037696 WO2005047482A2 (fr) | 2003-11-12 | 2004-11-12 | Systemes de detection de cellules electroniques en temps reel pour des epreuves a base de cellules |
US10/987,732 | 2004-11-12 | ||
USPCT/US2004/037696 | 2004-11-12 | ||
US63007104P | 2004-11-22 | 2004-11-22 | |
US63013104P | 2004-11-22 | 2004-11-22 | |
US60/630,131 | 2004-11-22 | ||
US60/630,071 | 2004-11-22 | ||
US63080904P | 2004-11-24 | 2004-11-24 | |
US60/630,809 | 2004-11-24 | ||
US63301904P | 2004-12-03 | 2004-12-03 | |
US60/633,019 | 2004-12-03 | ||
US64707505P | 2005-01-26 | 2005-01-26 | |
US64718905P | 2005-01-26 | 2005-01-26 | |
US64715905P | 2005-01-26 | 2005-01-26 | |
US60/647,189 | 2005-01-26 | ||
US60/647,075 | 2005-01-26 | ||
US60/647,159 | 2005-01-26 | ||
USPCT/US2005/004481 | 2005-02-09 | ||
US11/055,639 | 2005-02-09 | ||
PCT/US2005/004481 WO2005077104A2 (fr) | 2004-02-09 | 2005-02-09 | Systemes de detection de cellules electroniques en temps reel et applications en matiere de profilage de cytotoxicite et dosages de composes |
US11/055,639 US7560269B2 (en) | 2002-12-20 | 2005-02-09 | Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays |
US65390405P | 2005-02-17 | 2005-02-17 | |
US60/653,904 | 2005-02-17 | ||
US66089805P | 2005-03-10 | 2005-03-10 | |
US66082905P | 2005-03-10 | 2005-03-10 | |
US60/660,898 | 2005-03-10 | ||
US60/660,829 | 2005-03-10 | ||
US67367805P | 2005-04-21 | 2005-04-21 | |
US60/673,678 | 2005-04-21 | ||
US68942205P | 2005-06-10 | 2005-06-10 | |
US60/689,422 | 2005-06-10 | ||
PCT/US2005/027943 WO2006017762A2 (fr) | 2004-08-04 | 2005-08-04 | Controle dynamique de l'activation de la proteine g (gpcr) et de recepteur tyrosine kinase (rtk) dans des cellules vivantes mettant en oeuvre la technologie de detection micro-electronique de cellules en temps reel |
US11/197,994 | 2005-08-04 | ||
PCT/US2005/027891 WO2006015387A2 (fr) | 2004-08-04 | 2005-08-04 | Procede de dosage de la destruction de cellules cibles par des cellules tueuses naturelles, des lymphocytes t cytotoxiques et des neutrophiles, a l'aide de la technique de detection micro-electronique de cellules en temps reel |
US11/198,831 | 2005-08-04 | ||
USPCT/US2005/027943 | 2005-08-04 | ||
US11/198,831 US8263375B2 (en) | 2002-12-20 | 2005-08-04 | Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology |
US11/197,994 US7468255B2 (en) | 2002-12-20 | 2005-08-04 | Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology |
USPCT/US2005/027891 | 2005-08-04 | ||
PCT/US2005/034561 WO2006036952A2 (fr) | 2004-09-27 | 2005-09-27 | Surveillance dynamique de l'adhesion et de la diffusion cellulaire a l'aide du systeme rt-ces |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2580548A1 true CA2580548A1 (fr) | 2006-04-06 |
Family
ID=56290735
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002580548A Abandoned CA2580548A1 (fr) | 2004-09-27 | 2005-09-27 | Surveillance dynamique de l'adhesion et de la diffusion cellulaire a l'aide du systeme rt-ces |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1800312A4 (fr) |
CA (1) | CA2580548A1 (fr) |
WO (1) | WO2006036952A2 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2199783A1 (fr) * | 2008-12-17 | 2010-06-23 | Koninklijke Philips Electronics N.V. | Dispositif microélectronique pour la mesure de l'adhésion cellulaire |
CN111172024A (zh) * | 2019-12-31 | 2020-05-19 | 福建农林大学 | 一种原位表征种间直接电子传递的装置 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5981268A (en) * | 1997-05-30 | 1999-11-09 | Board Of Trustees, Leland Stanford, Jr. University | Hybrid biosensors |
US6132683A (en) * | 1998-12-23 | 2000-10-17 | Matsushita Electric Industrial Co., Ltd. | Cell potential measuring electrode and measuring apparatus using the same |
US20030166015A1 (en) * | 1999-04-15 | 2003-09-04 | Zarowitz Michael A. | Multiplexed analysis of cell-substrate interactions |
US7306924B2 (en) * | 2000-04-17 | 2007-12-11 | Purdue Research Foundation | Biosensor and related method |
US20040146849A1 (en) * | 2002-01-24 | 2004-07-29 | Mingxian Huang | Biochips including ion transport detecting structures and methods of use |
EP1539992B1 (fr) * | 2002-07-20 | 2009-11-11 | Acea Biosciences, Inc. | Dispositifs a base d'impedance et procedes a utiliser dans des dosages |
-
2005
- 2005-09-27 CA CA002580548A patent/CA2580548A1/fr not_active Abandoned
- 2005-09-27 EP EP05812268A patent/EP1800312A4/fr not_active Withdrawn
- 2005-09-27 WO PCT/US2005/034561 patent/WO2006036952A2/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2006036952A2 (fr) | 2006-04-06 |
WO2006036952A3 (fr) | 2006-12-21 |
EP1800312A4 (fr) | 2011-10-05 |
EP1800312A2 (fr) | 2007-06-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7732127B2 (en) | Dynamic monitoring of cell adhesion and spreading using the RT-CES system | |
US11604197B2 (en) | Real time electronic cell sensing systems and applications for cell-based assays | |
US7468255B2 (en) | Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology | |
US7876108B2 (en) | Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays | |
EP1773980B1 (fr) | Procede de dosage de la destruction de cellules cibles par des cellules tueuses naturelles, des lymphocytes t cytotoxiques et des neutrophiles, a l'aide de la technique de detection micro-electronique de cellules en temps reel | |
US10067121B2 (en) | Dynamic monitoring of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) activity and pathways in living cells using real-time microelectronic cell sensing technology | |
US8041515B2 (en) | Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents | |
CA2556219A1 (fr) | Methodes de dosage cellulaire par controle de l'impedance du substrat cellulaire | |
CA2580548A1 (fr) | Surveillance dynamique de l'adhesion et de la diffusion cellulaire a l'aide du systeme rt-ces | |
EP2450698B1 (fr) | Système de détection de cellule électronique en temps réel et application pour le profilage de la cytotoxicité et l'analyse de composés | |
US20230288429A1 (en) | Real time electronic cell sensing systems and applications for cell-based assays | |
EP2064644A2 (fr) | Utilisation du profilage cytologique base sur l'impedance pour classer des profils de reponses cellulaires lors d'une exposition a des agents biologiquement actifs |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Dead |