CA2573933A1 - Method to measure dynamic internal calibration true dose response curves - Google Patents
Method to measure dynamic internal calibration true dose response curves Download PDFInfo
- Publication number
- CA2573933A1 CA2573933A1 CA002573933A CA2573933A CA2573933A1 CA 2573933 A1 CA2573933 A1 CA 2573933A1 CA 002573933 A CA002573933 A CA 002573933A CA 2573933 A CA2573933 A CA 2573933A CA 2573933 A1 CA2573933 A1 CA 2573933A1
- Authority
- CA
- Canada
- Prior art keywords
- analyte
- test
- calibration
- printed
- assay device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A method of determining an amount of analyte in a sample solution is provided.
The method involves the use of an assay device that has a substantially planar assay surface. The surface has a plurality of calibration dots a test dot printed thereon. The calibration dots contain pre-determined quantities of the analyte while the test dot includes a capture antibody for binding to the analyte. The analyte is mixed into a solution having a sample antibody for the analyte, where the antibody is labeled with a detectable marker. The sample solution is introduced into the loading portion of the assay device for delivery to said reading portion. The next step is to measure the intensity of detectable marker in the calibration dots. With the data obtained, one then prepares a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker. The intensity of detectable marker in the test dot can be measures and the amount of anayte present in said test dot calculated by comparing the intensity of detectable marker to the amount of analyte corresponding to the intensity in said calibration curve.
The method involves the use of an assay device that has a substantially planar assay surface. The surface has a plurality of calibration dots a test dot printed thereon. The calibration dots contain pre-determined quantities of the analyte while the test dot includes a capture antibody for binding to the analyte. The analyte is mixed into a solution having a sample antibody for the analyte, where the antibody is labeled with a detectable marker. The sample solution is introduced into the loading portion of the assay device for delivery to said reading portion. The next step is to measure the intensity of detectable marker in the calibration dots. With the data obtained, one then prepares a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker. The intensity of detectable marker in the test dot can be measures and the amount of anayte present in said test dot calculated by comparing the intensity of detectable marker to the amount of analyte corresponding to the intensity in said calibration curve.
Claims (20)
1. A method of determining an amount of an analyte in a sample comprising the following steps:
.cndot. providing an assay device having a surface, said surface having a plurality of calibration dots printed thereon and a test dot printed thereon, the calibration dots including pre-determined quantities of the analyte, the test dot including a reagent for binding to said analyte;
.cndot. providing a solution having a sample reagent specific for the analyte, said sample reagent being labeled with a detectable marker;
.cndot. introducing the sample into said solution to form a sample solution;
.cndot. introducing said sample solution onto said assay device;
.cndot. measuring an intensity of detectable marker in said calibration dots;
.cndot. preparing a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker;
.cndot.measuring an intensity of detectable marker in said test dot; and .cndot.calculating an amount of analyte present in said test dot by comparing the intensity of detectable marker to the amount of analyte corresponding to said intensity in said calibration curve.
.cndot. providing an assay device having a surface, said surface having a plurality of calibration dots printed thereon and a test dot printed thereon, the calibration dots including pre-determined quantities of the analyte, the test dot including a reagent for binding to said analyte;
.cndot. providing a solution having a sample reagent specific for the analyte, said sample reagent being labeled with a detectable marker;
.cndot. introducing the sample into said solution to form a sample solution;
.cndot. introducing said sample solution onto said assay device;
.cndot. measuring an intensity of detectable marker in said calibration dots;
.cndot. preparing a calibration curve correlating the amount of analyte in said calibration dots to said intensity of detectable marker;
.cndot.measuring an intensity of detectable marker in said test dot; and .cndot.calculating an amount of analyte present in said test dot by comparing the intensity of detectable marker to the amount of analyte corresponding to said intensity in said calibration curve.
2. A method according to claim 1 wherein the reagent is a capture antibody and the sample reagent is an antibody that binds specifically to said analyte.
3. A method according to claim 1 or claim 2 wherein the assay device has a loading portion for receiving the sample solution and a reading portion, the reading portion having the plurality of calibration dots printed thereon and the test dot printed thereon.
4. A method according to one of claims 1-3 wherein the calibration dots are printed in a volume ranging from 1 picoliter to 1 microliter.
5. A method according to one of claims 1-3 wherein the volume ranges from 1 picoliter to 4 picoliters.
6. A method according to one of claims 1-5 wherein the calibration dots are printed in a volume ranging from 1 picometers to 12 millimeters.
7. A method according to one of claims 1-6 wherein the calibration dots are printed in a volume ranging from 25 micrometers to 300 micrometers.
8. A method according to one of claims 1-7 wherein the calibration dots are printed in arrays at predetermined X-Y co-ordinates.
9. A method according to one of claims 1-8 wherein the calibration arrays are printed at pre-determined concentrations.
10. A method according to claim 1 wherein the calibration dots are printed at concentrations to encompass a dynamic range of test substrate to be found according to the test sample.
11. A method according to claim 1 wherein the calibration arrays are printed at concentrations to encompass a dynamic range of test substrate to be found according to the test sample.
12. A method according to claim 1 wherein the surface of the assay device is substantially planar.
13. A method according to claim 1 wherein the calibration dots have a concentration of the analyte that corresponds to a dynamic range of the analyte.
14. A method according to claim 1 wherein the calibration dots are arranged in at least three arrays for carrying out three or more replicates of a test.
15. A method according to claim 1 wherein the test dots are arranged in at least three arrays for carrying out three or more replicates of the same test.
16. An assay device according to claim 14 or claim 15 wherein the test may be the same or different.
17. A method according to claim 1 wherein a single analyte in a single test is measured on the assay device.
18. A method according to claim 1 wherein a single analyte in a plurality of arrays for same test is measured on the assay device.
19. A method according to claim 1 wherein different analytes are measured contemporaneously on the assay device.
20. A method and assay device according to claim 1 wherein a plurality of different analytes in a plurality of different tests are measured contemporaneously on the assay device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2573933A CA2573933C (en) | 2004-07-20 | 2005-07-20 | Method to measure dynamic internal calibration true dose response curves |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002475240A CA2475240A1 (en) | 2004-07-20 | 2004-07-20 | Method and device to measure dynamic internal calibration true dose response curves |
| CA2,475,240 | 2004-07-20 | ||
| CA2573933A CA2573933C (en) | 2004-07-20 | 2005-07-20 | Method to measure dynamic internal calibration true dose response curves |
| PCT/CA2005/001147 WO2006007726A1 (en) | 2004-07-20 | 2005-07-20 | Method to measure dynamic internal calibration true dose response curves |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2573933A1 true CA2573933A1 (en) | 2007-01-12 |
| CA2573933C CA2573933C (en) | 2010-09-14 |
Family
ID=37872143
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2573933A Active CA2573933C (en) | 2004-07-20 | 2005-07-20 | Method to measure dynamic internal calibration true dose response curves |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2573933C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010075632A1 (en) * | 2008-12-29 | 2010-07-08 | Sqi Diagnostics Systems Inc. | Methods for multiplex analyte detection and quantification |
| US11422129B2 (en) | 2004-07-20 | 2022-08-23 | Sqi Diagnostics Systems Inc. | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
-
2005
- 2005-07-20 CA CA2573933A patent/CA2573933C/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11422129B2 (en) | 2004-07-20 | 2022-08-23 | Sqi Diagnostics Systems Inc. | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
| WO2010075632A1 (en) * | 2008-12-29 | 2010-07-08 | Sqi Diagnostics Systems Inc. | Methods for multiplex analyte detection and quantification |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2573933C (en) | 2010-09-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101044402B (en) | Method to measure dynamic internal calibration true dose response curves | |
| US11287424B2 (en) | Calibrating assays using reaction time | |
| EP1251351A2 (en) | Method and apparatus for portable product authentication | |
| KR890702033A (en) | How to measure the concentration of surrounding analytes | |
| US20180120310A1 (en) | Method of and System for Printing In-Well Calibration Features | |
| US20220048025A1 (en) | Systems and methods to enhance consistency of assay performance | |
| CN110672594A (en) | Chip colorimetric detection method based on light transmission result analysis | |
| DE602005026024D1 (en) | METHOD AND DEVICE FOR OPTIMIZING ANALYTE AND ANTIBODY SUBSTRATE BINDING THROUGH SMALL ENERGY ADSORPTION | |
| CN108535472B (en) | Detection test strip capable of remarkably improving lateral flow immunochromatography | |
| CA2573933A1 (en) | Method to measure dynamic internal calibration true dose response curves | |
| WO2002077645A3 (en) | Method for the measurement of soluble analytes | |
| Klapproth et al. | Development of a multi-analyte CMOS sensor for point-of-care testing | |
| WO2003031976A3 (en) | Calibrating microarrays | |
| KR101356628B1 (en) | Simultaneous multiple biomarker detection method for immunoassay and apparatus thereof | |
| CN1936581A (en) | Biochip and analytical instrument for analyzing same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |