CA2573931A1 - Method and device to optimize analyte and antibody substrate binding by least energy adsorption - Google Patents
Method and device to optimize analyte and antibody substrate binding by least energy adsorption Download PDFInfo
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- CA2573931A1 CA2573931A1 CA002573931A CA2573931A CA2573931A1 CA 2573931 A1 CA2573931 A1 CA 2573931A1 CA 002573931 A CA002573931 A CA 002573931A CA 2573931 A CA2573931 A CA 2573931A CA 2573931 A1 CA2573931 A1 CA 2573931A1
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- analyte
- assay
- binding
- site specific
- assay device
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
- G01N33/552—Glass or silica
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00351—Means for dispensing and evacuation of reagents
- B01J2219/00387—Applications using probes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/0061—The surface being organic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/00626—Covalent
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00623—Immobilisation or binding
- B01J2219/0063—Other, e.g. van der Waals forces, hydrogen bonding
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
- B01J2219/00632—Introduction of reactive groups to the surface
- B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
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- B01J2219/00662—Two-dimensional arrays within two-dimensional arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00693—Means for quality control
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00718—Type of compounds synthesised
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- B01J2219/00725—Peptides
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
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- B01J2219/00727—Glycopeptides
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00731—Saccharides
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides a method of making an assay device for conducting an assay to detect a concentration of an analyte in a sample fluid.
The assay devices would typically have a substantially planar surface having a series of site specific immobilized calibration spot arrays containing pre-determined quantities of the analyte printed thereon. In addition, a series of site specific immobilized test spot arrays, including capture antibody for binding the analyte protein is printed on the assay device. The method involves first modifying the planar surface to provide hydrophobic binding sites, hydrophilic linking and covalent bonding sites. Then the method requires printing the series of site specific immobilized test spot arrays and the series of site specific immobilized calibration spot arrays on the substantially planar surface. Applying the sample fluid to the assay device is the next step followed by testing a sensitivity of the assay and modulating ratios of the hydrophobic, hydrophilic and covalent binding sites in order to optimize the sensitivity of the assay.
The assay devices would typically have a substantially planar surface having a series of site specific immobilized calibration spot arrays containing pre-determined quantities of the analyte printed thereon. In addition, a series of site specific immobilized test spot arrays, including capture antibody for binding the analyte protein is printed on the assay device. The method involves first modifying the planar surface to provide hydrophobic binding sites, hydrophilic linking and covalent bonding sites. Then the method requires printing the series of site specific immobilized test spot arrays and the series of site specific immobilized calibration spot arrays on the substantially planar surface. Applying the sample fluid to the assay device is the next step followed by testing a sensitivity of the assay and modulating ratios of the hydrophobic, hydrophilic and covalent binding sites in order to optimize the sensitivity of the assay.
Claims (19)
1. A method of making an assay device for conducting an assay to detect a concentration of an analyte in a sample fluid, said assay device having a surface, the surface having a site specific immobilized calibration dot including a pre-determined quantity of the analyte printed thereon and a test dot including a capture antibody for binding said analyte, said method including the following steps:
.cndot. modifying said surface to provide hydrophobic binding sites, hydrophilic linking sites and covalent linking sites;
.cndot. printing said test spots and said calibration dots on said surface;
.cndot. applying the sample test fluid to the assay device;
.cndot. testing a sensitivity of the assay; and .cndot. modulating the ratio of said hydrophobic binding sites to said hydrophilic linking to said covalent sites in order to optimize the sensitivity of the assay.
.cndot. modifying said surface to provide hydrophobic binding sites, hydrophilic linking sites and covalent linking sites;
.cndot. printing said test spots and said calibration dots on said surface;
.cndot. applying the sample test fluid to the assay device;
.cndot. testing a sensitivity of the assay; and .cndot. modulating the ratio of said hydrophobic binding sites to said hydrophilic linking to said covalent sites in order to optimize the sensitivity of the assay.
2. A method according to claim 1 wherein said surface is substantially planar.
3. A method according to claim 2 wherein said surface includes a loading portion for receiving the sample and a reading portion for receiving said sample from the loading portion, the calibration dots and the test dot being printed on said reading portion.
4. A method according to claim 2wherein the test spots and the calibration spots are printed on the surface under conditions of constant relative humidity.
5. A method according to claim 4wherein the thickness of the test dots and the calibration dots is about a thickness of a single molecular layer of adsorbate.
6. A method according to claim 4 wherein the constant relative humidity is in the range of 15% to 90%.
7. A method according to claim 1 wherein a ratio of said hydrophobic binding sites to said hydrophilic to said covalent site charges is in the relative range to ensure optimal binding ratio adsorption of analyte with optimal binding, while maintaining optimal attachment and function of marker antibody.
8. A method according to claim 1, to ensure optimal adsorption of capture antibody without causing structural and spatial realignment of antibody structure to inhibit antibody-analyte binding.
9. A method according to claim 1, wherein the site specific immobilized spot contains a molecular spacer.
10. A method according to claim 10, wherein the site specific immobilized dot displays evenly dispersed analyte.
11. A method according to claim 10, wherein the site specific immobilized dot displays evenly dispersed analyte with minimized stearic hindrance.
12. A method according to claim 1, wherein the site specific immobilized dot includes a surface modulating buffer optimized to obtain optimal binding of conjugate and complex-conjugate analyte aggregates.
13. A method according to claim 1 wherein said surface includes substrates for binding of DNA, proteins, glycoproteins, lipopolysacchandes, peptides, drugs, lectins and other molecules through amino- thiol-, and hydroxyl functional groups, said groups being epoxysilane-activated.
14. A method according to claim 13 wherein said surface includes epoxysilane-activated glass for covalent binding of DNA, proteins, glycoproteins, lipopolysaccharides, peptides, drugs, lectins and other molecules through amino-thiol-, and hydroxyl functional groups.
15. A method according to claim 1 wherein the assay device includes a multiplex of immobilized gradient test dot arrays and calibration dot arrays.
16. A method according to claim 15, further comprising the steps of introducing a first and second samples into assay device and contacting the site specific immobilized dot arrays with the second sample, the second sample containing a specified substance.
17. A method according to claim 1, wherein the assay device is configured for optimal fluid flow contact angle to ensure adequate and controlled flow rate of test fluid in the device.
18. A method according to claim 1, wherein the assay device is configured to ensure optimal adsorption of analyte without causing structural and spatial realignment of analyte structure to inhibit antibody-analyte binding.
19. A method according to claim 1 wherein the assay device is includes a plurality sample compartments, the sample compartments comprising an integral structure, each of the compartments including a multiplex of immobilized gradient test dot arrays and calibration dot arrays printed thereon.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2573931A CA2573931C (en) | 2004-07-20 | 2005-07-20 | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2,475,456 | 2004-07-20 | ||
CA002475456A CA2475456A1 (en) | 2004-07-20 | 2004-07-20 | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
CA2573931A CA2573931C (en) | 2004-07-20 | 2005-07-20 | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
PCT/CA2005/001142 WO2006007722A2 (en) | 2004-07-20 | 2005-07-20 | Method to optimize analyte and antibody substrate binding by least energy adsorption |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2573931A1 true CA2573931A1 (en) | 2006-01-26 |
CA2573931C CA2573931C (en) | 2012-09-18 |
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ID=37872142
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2573931A Active CA2573931C (en) | 2004-07-20 | 2005-07-20 | Method and device to optimize analyte and antibody substrate binding by least energy adsorption |
Country Status (1)
Country | Link |
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CA (1) | CA2573931C (en) |
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2005
- 2005-07-20 CA CA2573931A patent/CA2573931C/en active Active
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Publication number | Publication date |
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CA2573931C (en) | 2012-09-18 |
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