CA2572777A1 - Medicament comprising corticotropin releasing factor and proopiomelanocortin, and uses thereof - Google Patents
Medicament comprising corticotropin releasing factor and proopiomelanocortin, and uses thereof Download PDFInfo
- Publication number
- CA2572777A1 CA2572777A1 CA002572777A CA2572777A CA2572777A1 CA 2572777 A1 CA2572777 A1 CA 2572777A1 CA 002572777 A CA002572777 A CA 002572777A CA 2572777 A CA2572777 A CA 2572777A CA 2572777 A1 CA2572777 A1 CA 2572777A1
- Authority
- CA
- Canada
- Prior art keywords
- disease
- crf
- pomc
- serum
- neuropathy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- A61K38/34—Melanocyte stimulating hormone [MSH], e.g. alpha- or beta-melanotropin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/095—Oxytocins; Vasopressins; Related peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- A61K38/35—Corticotropin [ACTH]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/06—Antipsoriatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/04—Centrally acting analgesics, e.g. opioids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/06—Antimigraine agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/08—Antiepileptics; Anticonvulsants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/24—Antidepressants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/04—Anorexiants; Antiobesity agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/57509—Corticotropin releasing factor [CRF] (Urotensin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
Analysis of a goat serum product with many therapeutic effects is described.
The product is identified as containing proopiomelanocortin (POMC) and Corticotropin releasing factor (CRF) peptides, as well as breakdown products of these peptides. We describe methods of treatment of diseases including cancers, multiple sclerosis, and neural disorders using these peptides and their products, as well as medicaments including such peptides and methods of producing the peptides.
The product is identified as containing proopiomelanocortin (POMC) and Corticotropin releasing factor (CRF) peptides, as well as breakdown products of these peptides. We describe methods of treatment of diseases including cancers, multiple sclerosis, and neural disorders using these peptides and their products, as well as medicaments including such peptides and methods of producing the peptides.
Description
Medicament FIELD OF THE INVENTION
The present invention relates to a medicament, and in particular to a pharmaceutical composition. The medicament is considered particularly suited to treatment of neural disorders, although a number of other disorders may be treatable with the invention. Aspects of the invention also relate to methods of preparation of such a medicament, and to methods of treatment of disorders using said medicament.
BACKGROUND TO THE INVENTION
PCT publications W003/004049 and W003/064472 describe therapeutic agents and treatments which are based on a serum composition with many surprising beneficial effects. The respective content of each of these two texts is incorporated in full by specific reference. In particular, the reader is referred to them for an understanding of how the therapeutic agent can be prepared, and for the indications which can be treated.
Typically a goat is immunised with H> V-3B viral lysate raised in H9 cells.
The resulting serum is believed to be active against, among other disorders, multiple sclerosis. The reader is further referred in particular to the section on pages 3 and 4 of W003/004049 headed 'Example of Production of Goat Serum' for further details of the production of serum. This section is incorporated herein by reference. A brief summary is given below.
The present invention relates to a medicament, and in particular to a pharmaceutical composition. The medicament is considered particularly suited to treatment of neural disorders, although a number of other disorders may be treatable with the invention. Aspects of the invention also relate to methods of preparation of such a medicament, and to methods of treatment of disorders using said medicament.
BACKGROUND TO THE INVENTION
PCT publications W003/004049 and W003/064472 describe therapeutic agents and treatments which are based on a serum composition with many surprising beneficial effects. The respective content of each of these two texts is incorporated in full by specific reference. In particular, the reader is referred to them for an understanding of how the therapeutic agent can be prepared, and for the indications which can be treated.
Typically a goat is immunised with H> V-3B viral lysate raised in H9 cells.
The resulting serum is believed to be active against, among other disorders, multiple sclerosis. The reader is further referred in particular to the section on pages 3 and 4 of W003/004049 headed 'Example of Production of Goat Serum' for further details of the production of serum. This section is incorporated herein by reference. A brief summary is given below.
Preparation of serum Approximately 400 cc of blood is taken from a goat under sterile technique.
The animal may typically be re-bled in 10 to 14 days, once the volume of blood is replenished. A pre-bleeding regime may be useful to stimulate production of the active components of the serum. The blood is then centrifuged to separate the serum, and the serum filtered to remove large clots and particulate matter.
The serum is then treated with supersaturated ammonium sulphate (47% solution at 4 C) to precipitate antibodies and other material. The resulting solution is centrifuged in a Beckman J6M/E centrifuge at 3500 rpm for 45 minutes, after which the supernatant fluid is removed. The precipitated immunoglobulin and other solid material are resuspended in PBS buffer (phosphate buffered saline) sufficient to redissolve the precipitate.
The solution is then subjected to diafiltration against a PBS buffer with a molecular weight cut-off of 10,000 Daltons. at 4 C. After diafiitration the product is fiitered through a 0.2 micron filter into a sterile container and adjusted to a protein concentration of 4mg/ml. The solution is put into vials to give single doses of Iml, and stored at -22 C prior to use. This product is referred to herein as the serum composition, the composition, or the product, while treatment of a patient involves administering the composition to the patient by an appropriate route (usually subcutaneously).
The use of HIV-3B viral lysate as an immunogen is not believed to be essential for the production of active serum; it is believed that a medium which has been used for growth of a viral culture, or which is suitable for such growth, may also produce a suitable response when used as an immunogen. The supernate of a cell culture growth medium such as PBMC or the cancer immortal cell line as used to grow HIV-3B are given as an example. The HIV or other virus does not need to be present to produce an effective immunogen to create the composition. Other suitable immunogens are recited on pages 12 and 13 of W003/064472.
A pyrogenic material (for example, RIBI or Freund's adjuvant) can be used to promote production of the active component in the serum. Another possible factor may be exposure of the animal to daylight, with greater daylight hours (or exposure to daylight equivalent) may increase active component serum levels.
Uses of the serum composition The composition is believed to be effective against a number of disorders, in particular multiple sclerosis. Reference is also made in the previously-identified publications to the composition as being useful in the treatment of inflammatory diseases such as rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases; axonal or nerve damage; and cancers.
The composition is also believed to cause a reduction in viral load in HIV
patients, and an increase in CD4+ cells.
Several other diseases which may be treated by the composition are described, and the reader is referred to these earlier publications for a full understanding of the range and nature of conditions which may be treated. In particular, the contents of W003/004049 and W003/064472 are specifically incorporated herein by reference.
A non-exhaustive list of disorders against which the serum composition is believed to be effective, in addition to those mentioned above, includes cancers, in particular myelomas, melanomas, and lymphomas; cardiovascular diseases; and neural disorders, both demyelinating and non-demyelinating.
Examples of disorders which may be treated in accordance with the present invention include cerebrovascular ischaemic disease; Alzheimer's disease;
The animal may typically be re-bled in 10 to 14 days, once the volume of blood is replenished. A pre-bleeding regime may be useful to stimulate production of the active components of the serum. The blood is then centrifuged to separate the serum, and the serum filtered to remove large clots and particulate matter.
The serum is then treated with supersaturated ammonium sulphate (47% solution at 4 C) to precipitate antibodies and other material. The resulting solution is centrifuged in a Beckman J6M/E centrifuge at 3500 rpm for 45 minutes, after which the supernatant fluid is removed. The precipitated immunoglobulin and other solid material are resuspended in PBS buffer (phosphate buffered saline) sufficient to redissolve the precipitate.
The solution is then subjected to diafiltration against a PBS buffer with a molecular weight cut-off of 10,000 Daltons. at 4 C. After diafiitration the product is fiitered through a 0.2 micron filter into a sterile container and adjusted to a protein concentration of 4mg/ml. The solution is put into vials to give single doses of Iml, and stored at -22 C prior to use. This product is referred to herein as the serum composition, the composition, or the product, while treatment of a patient involves administering the composition to the patient by an appropriate route (usually subcutaneously).
The use of HIV-3B viral lysate as an immunogen is not believed to be essential for the production of active serum; it is believed that a medium which has been used for growth of a viral culture, or which is suitable for such growth, may also produce a suitable response when used as an immunogen. The supernate of a cell culture growth medium such as PBMC or the cancer immortal cell line as used to grow HIV-3B are given as an example. The HIV or other virus does not need to be present to produce an effective immunogen to create the composition. Other suitable immunogens are recited on pages 12 and 13 of W003/064472.
A pyrogenic material (for example, RIBI or Freund's adjuvant) can be used to promote production of the active component in the serum. Another possible factor may be exposure of the animal to daylight, with greater daylight hours (or exposure to daylight equivalent) may increase active component serum levels.
Uses of the serum composition The composition is believed to be effective against a number of disorders, in particular multiple sclerosis. Reference is also made in the previously-identified publications to the composition as being useful in the treatment of inflammatory diseases such as rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases; axonal or nerve damage; and cancers.
The composition is also believed to cause a reduction in viral load in HIV
patients, and an increase in CD4+ cells.
Several other diseases which may be treated by the composition are described, and the reader is referred to these earlier publications for a full understanding of the range and nature of conditions which may be treated. In particular, the contents of W003/004049 and W003/064472 are specifically incorporated herein by reference.
A non-exhaustive list of disorders against which the serum composition is believed to be effective, in addition to those mentioned above, includes cancers, in particular myelomas, melanomas, and lymphomas; cardiovascular diseases; and neural disorders, both demyelinating and non-demyelinating.
Examples of disorders which may be treated in accordance with the present invention include cerebrovascular ischaemic disease; Alzheimer's disease;
Huntingdon's chorea; mixed connective tissue diseases; scieroderma;
anaphylaxis; septic shock; carditis and endocarditis; wound healing; contact dermatitis; occupational lung diseases; glomerulnephritis; transplant rejection;
temporal arteritis; vasculitic diseases; hepatitis; and burns. All of these disorders may have an inflammatory component, but are believed to be additionally treatable based on the non-demyelinating neural aspect of the disorder. Further non-demyelinating disorders which may be treated, and which are considered to have a degenerative component include multiple system atrophy; epilepsy; muscular dystrophy; schizophrenia; bipolar disorder; and depression. Other non-demyelinating disorders which may be treated include channelopathies; myaesthenia gravis; pain due to malignant neoplasia; chronic fatigue syndrome; fibromyositis; irritable bowel syndrome; work related upper limb disorder; cluster headache; migraine; and chronic daily headache.
Demyelinating disorders which may be treatable include infections of the nervous system; nerve entrapment and focal injury; traumatic spinal cord injury; brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy;
channelopathies; and tic douloureux.
The composition may be useful in the treatment of autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis.
The composition is also believed to be effective against inflammatory conditions.
The composition is useful in the treatment of all kinds of peripheral neuropathy of axonal and demyelinating type, including hereditary motor and sensor neuropathy of all types; Charcot-Marie Tooth disease (CMT) types CMT1A, CMT1B, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy / Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies 5 (HSANI, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSAN5); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease;
Fabry's Disease; Adrerioleukodystrophy; Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCAIO, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia; Cockayne's syndrome; and Giant axonal neuropathy.
The composition may also be useful in the treatment of chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome.
The composition may also have anti-angiogenic properties, caused by the molecules thrombospondin-1 (TSP-1) and platelet factor-4 (PF-4).
It is believed that the composition may also be effective for treatment of animals, in particular, but not exclusively, the treatment of canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders.
Nature of the serum Although the serum composition has exhibited many surprising effects, and has been studied extensively, until now the active component or components of the serum have not yet been identii"led. This has been disadvantageous, both in terms of isolating the active component for further study, and in terms of exploring possible alternative sources of the active component or components.
Further, it has been necessary to administer the treatment to patients as serum, which necessitates injections, and imposes certain restrictions on the handling and processing of the composition. It is believed that the serum has bioactive components sensitive to protease degradation.
We have now identified a number of potential active components of the serum.
This identification allows the manufacture of novel pharmaceutical compositions comprising one or more of the active components in various forms, and the treatment of one or more of the disorders recited above and in our earlier patent applications using the active component(s). The identification also opens up novel approaches for treating the various disorders based on the active component(s) and not simply the serum itself.
SUMMARY OF THE INVENTION
The invention resides in a bioactive composition which triggers a molecular cascade in treated patients.
According to a first aspect of the present invention, there is provided a pharmaceutical composition comprising a corticotropin releasing factor (CRF) peptide. CRF is also known as corticotropin releasing hormone (CRH).
CRF is a peptide produced in the hypothalamus, and is believed to be involved in stress response. Human CRF is described in detail in entry 122560 of OMIM
(online mendelian inheritance in man, accessible through http=.Ilwww.ncbi.nim.nih.goyL). The nucleotide and amino acid sequence of human CRF is also known, and has GENBANK accession number BC011031.
Knowledge of the sequence and size data for human CRF will allow the skilled person to determine the equivalent information for non-human CRF, including goat CRF.
By "a CRF peptide" is meant any peptide having a corresponding sequence, structure, or function. It will be apparent to the skilled person that the canonical nucleotide and/or amino acid sequences given for human CRF in the GENBANK entry referenced above may be varied to a certain degree without affecting the structure or function of the peptide. In particular, allelic variants and functional mutants are included within this definition. Mutants may include conservative amino acid substitutions; and fragments and derivatives of CRF.
Administration of CRF to a patient is believed to stimulate production of endogenous CRF, which in turn stimulates production of proopiomelanocortin (POMC) and its related component peptides.
POMC is a peptide (prohormone) produced in the pituitary gland (as well as a number of other organs, certain tumours such as melanomas, and normal skin cells) which is the precursor of a set of corticotrophic hormones which exert a number of effects on the host. POMC is the precursor to alpha, beta, and gamma melanocyte stimulating hormone (MSH); adrenocorticotrophin (ACTH);
beta and gamma lipotropin (LPH); and beta endorphin. All of these hormones are cleaved from a single large precursor, POMC, and are termed herein "POMC
products".
Preferably the pharmaceutical composition comprises non-human CRF;
conveniently ungulate CRF; and most preferably goat CRF. It has been surprisingly identified that goat serum contains CRF, particularly when the goat is stimulated by physiological stress, such as bleeding or immunization. This provides a convenient source for CRF for pharmaceutical compositions of the present invention. It is also believed that CRF may have a self-sustaining effect in the patient, in that administration of an initial amount of CRF leads to endogenous production of CRF in the patient; thus, an initial administration of a low level of CRF may have a significant effect on the patient, including an increase in the levels of POMC peptides.
anaphylaxis; septic shock; carditis and endocarditis; wound healing; contact dermatitis; occupational lung diseases; glomerulnephritis; transplant rejection;
temporal arteritis; vasculitic diseases; hepatitis; and burns. All of these disorders may have an inflammatory component, but are believed to be additionally treatable based on the non-demyelinating neural aspect of the disorder. Further non-demyelinating disorders which may be treated, and which are considered to have a degenerative component include multiple system atrophy; epilepsy; muscular dystrophy; schizophrenia; bipolar disorder; and depression. Other non-demyelinating disorders which may be treated include channelopathies; myaesthenia gravis; pain due to malignant neoplasia; chronic fatigue syndrome; fibromyositis; irritable bowel syndrome; work related upper limb disorder; cluster headache; migraine; and chronic daily headache.
Demyelinating disorders which may be treatable include infections of the nervous system; nerve entrapment and focal injury; traumatic spinal cord injury; brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy;
channelopathies; and tic douloureux.
The composition may be useful in the treatment of autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis.
The composition is also believed to be effective against inflammatory conditions.
The composition is useful in the treatment of all kinds of peripheral neuropathy of axonal and demyelinating type, including hereditary motor and sensor neuropathy of all types; Charcot-Marie Tooth disease (CMT) types CMT1A, CMT1B, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy / Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies 5 (HSANI, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSAN5); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease;
Fabry's Disease; Adrerioleukodystrophy; Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCAIO, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia; Cockayne's syndrome; and Giant axonal neuropathy.
The composition may also be useful in the treatment of chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome.
The composition may also have anti-angiogenic properties, caused by the molecules thrombospondin-1 (TSP-1) and platelet factor-4 (PF-4).
It is believed that the composition may also be effective for treatment of animals, in particular, but not exclusively, the treatment of canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders.
Nature of the serum Although the serum composition has exhibited many surprising effects, and has been studied extensively, until now the active component or components of the serum have not yet been identii"led. This has been disadvantageous, both in terms of isolating the active component for further study, and in terms of exploring possible alternative sources of the active component or components.
Further, it has been necessary to administer the treatment to patients as serum, which necessitates injections, and imposes certain restrictions on the handling and processing of the composition. It is believed that the serum has bioactive components sensitive to protease degradation.
We have now identified a number of potential active components of the serum.
This identification allows the manufacture of novel pharmaceutical compositions comprising one or more of the active components in various forms, and the treatment of one or more of the disorders recited above and in our earlier patent applications using the active component(s). The identification also opens up novel approaches for treating the various disorders based on the active component(s) and not simply the serum itself.
SUMMARY OF THE INVENTION
The invention resides in a bioactive composition which triggers a molecular cascade in treated patients.
According to a first aspect of the present invention, there is provided a pharmaceutical composition comprising a corticotropin releasing factor (CRF) peptide. CRF is also known as corticotropin releasing hormone (CRH).
CRF is a peptide produced in the hypothalamus, and is believed to be involved in stress response. Human CRF is described in detail in entry 122560 of OMIM
(online mendelian inheritance in man, accessible through http=.Ilwww.ncbi.nim.nih.goyL). The nucleotide and amino acid sequence of human CRF is also known, and has GENBANK accession number BC011031.
Knowledge of the sequence and size data for human CRF will allow the skilled person to determine the equivalent information for non-human CRF, including goat CRF.
By "a CRF peptide" is meant any peptide having a corresponding sequence, structure, or function. It will be apparent to the skilled person that the canonical nucleotide and/or amino acid sequences given for human CRF in the GENBANK entry referenced above may be varied to a certain degree without affecting the structure or function of the peptide. In particular, allelic variants and functional mutants are included within this definition. Mutants may include conservative amino acid substitutions; and fragments and derivatives of CRF.
Administration of CRF to a patient is believed to stimulate production of endogenous CRF, which in turn stimulates production of proopiomelanocortin (POMC) and its related component peptides.
POMC is a peptide (prohormone) produced in the pituitary gland (as well as a number of other organs, certain tumours such as melanomas, and normal skin cells) which is the precursor of a set of corticotrophic hormones which exert a number of effects on the host. POMC is the precursor to alpha, beta, and gamma melanocyte stimulating hormone (MSH); adrenocorticotrophin (ACTH);
beta and gamma lipotropin (LPH); and beta endorphin. All of these hormones are cleaved from a single large precursor, POMC, and are termed herein "POMC
products".
Preferably the pharmaceutical composition comprises non-human CRF;
conveniently ungulate CRF; and most preferably goat CRF. It has been surprisingly identified that goat serum contains CRF, particularly when the goat is stimulated by physiological stress, such as bleeding or immunization. This provides a convenient source for CRF for pharmaceutical compositions of the present invention. It is also believed that CRF may have a self-sustaining effect in the patient, in that administration of an initial amount of CRF leads to endogenous production of CRF in the patient; thus, an initial administration of a low level of CRF may have a significant effect on the patient, including an increase in the levels of POMC peptides.
Administration of pharmaceutical compositions of the invention may be accomplished orally or parenterally. Methods of parenteral delivery include topical, intra-arterial, intramuscular, subcutaneous, intramedullary, intrathecal, intraventricular, intravenous, intraperitoneal, or intranasal administration.
In addition to the active ingredients, such compositions may comprise suitable pharmaceutically acceptable carriers comprising excipients and other components which facilitate processing of the active compounds into preparations suitable for pharmaceutical administration.
Pharmaceutical compositions for oral administration can be formulated using pharmaceutically acceptable carriers known in the art in dosages suitable for oral administration. Such carriers enable the compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like suitable for ingestion by the subject.
Pharmaceutical preparations for oral use can be obtained through combination of active compounds with a solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable additional compounds if desired to obtain tablets or dragee cores. Suitable excipients include carbohydrate or protein fillers such as sugars, including lactose, sucrose, mannitol, sorbitol; starch from corn, wheat, rice, potato, or other plants; cellulose such as methylcelfulose, hydroxypropylmethylcellulose, or sodium carboxymethylcel[u[ose; and gums including arabic and tragacanth;
as well as proteins such as gelatin and coliagen. If desired, disintegrating or solubilising agents may be added, such as cross linked polyvinyl pyrrolidone, agar, alginic acid, or a salt thereof.
Dragee cores can be provided with suitable coatings such as concentrated sugar solutions, which may also contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterise the quantity of active compound.
Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating such as glycerol or sorbitol. Push-fit capsules can contain active ingredients mixed with a 1=liler or binders such as lactose or starches, lubricants such as talc or magnesium stearate, and, optionally stabilisers. In soft capsules, the active compounds can be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycol with or without stabilisers.
Pharmaceutical formulations for parenteral administration include aqueous solutions of active compounds. For injection, the pharmaceutical compositions of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiologically buffered saline. Aqueous suspension injections can contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Additionally, suspensions of the active compounds can be prepared as appropriate oily injection suspensions.
Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes.
Optionally, the suspension can also contain suitable stabilisers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.
For topical or nasal administration, penetrants appropriate to the particular barrier to be permeated may be used in the formulation.
The pharmaceutical compositions of the present invention can be manufactured substantially in accordance with standard manufacturing procedures known in the art.
The composition may also comprise one or more peptide regulatory or releasing factors, which may induce a cascade of release of further peptides by a variety of cells in the patient. Such additional factors are preferably derived from the 5 same source as the CRF, in particular goat serum. Suitable factors include aW
HLA, TGF-p, and IL-10, among others.
In preferred embodiments, the composition may comprise one or more of vasopressin, beta endorphin, and an enkephalin. In certain embodiments, the 10 composition may comprise CRF binding protein, CRF-BP. This binds CRF and may act as a reservoir for subsequent release of CRF to the patient.
The composition may further comprise a POMC peptide or a POMC product;
certain POMC products may be useful to administer to a patient to stimulate further production, or to obtain a desired response before endogenous POMC
can be produced.
Human POMC is described in detail in entry 176830 of OMIM (online mendelian inheritance in man, accessible through http=//www.ncbi.nlm.nih.gov/). The nucleotide and amino acid sequence of human POMC is also known, and has GENBANK accession number BC065832. Human POMC gives rise to a glycosylated protein precursor having a molecular weight of 31 kDa.
By "a POMC peptide" is meant any peptide having a corresponding sequence, structure, or function. It will be apparent to the skilled person that the canonical nucleotide and/or amino acid sequences given for human POMC in the GENBANK entry referenced above may be varied to a certain degree without affecting the structure or function of the peptide. In particular, allelic variants and functional mutants are included within this definition. Mutants may include conservative amino acid substitutions. "A POMC peptide" as used herein refers to any peptide acting as a precursor to at least one form of MSH, ACTH, at least one form of LPH, p endorphin, met-enkephalin and leu-enkephalin; and preferably all of a, p, and y MSH; ACTH; p and y LPH; and p endorphin, met-enkephalin and leu-enkephalin Although research has been carried out into the pharmaceutical potential of certain of the individual products of the POMC peptide, it is believed that administration of POMC itself has not been determined to have any medical use. It is possible that POMC itself is inactive, and must be cleaved into its products before having an activity.
Preferably the pharmaceutical composition comprises non-human POMC;
conveniently ungulate POMC; and most preferably goat POMC. Although POMC
is produced in the pituitary gland, and so would not be expected to be present in serum, at least at signihcant levels, it has been surprisingly identified that goat serum contains POMC, POMC-related peptides, and molecules associated with the POMC cascade, particularly when the goat is stimulated by physiological stress, such as bleeding or immunization. This provides a convenient source for POMC for pharmaceutical compositions of the present invention. It is also believed that POMC may have a self-sustaining effect in the patient, in that administration of an initial amount of POMC leads to endogenous production of POMC in the patient; thus, an initial administration of a low level of POMC may have a significant effect on the patient.
It is believed that, on administration of POMC and its associated molecules to a subject, the peptide is proteolysed to provide one or more of the products of POMC in a readily available form to the subject; there is also the induction of a molecular cascade which stimulates the hypotha[amo-pituitary-adrenal axis (HPA). This would be consistent with previously observed effects of the unpurihed goat serum; for example, a rapid 'buzz' effect on sublingual administration may be due to proteolysis of POMC to release p endorphin, which can then be absorbed through the mucous membranes. In addition, alpha MSH is known to have an effect on TL-10 and TGF-p production, which results in an anti-inflammatory effect, consistent with that which has been observed with goat serum. Alpha MSH is also known to inhibit the release of pro-inflammatory cytokines.
The composition may also have anti-inflammatory properties. We believe there is a passive transfer of anti-inflammatory response from the goat or other animal used to produce the serum to the patient. This is a consequence of the puriflcation process used to prepare the composition, in which a variety of active factors are retained in the serum. The composition may also comprise additional active components which provide an anti-inflammatory effect.
As mentioned above, it is believed that an initial administration of POMC
(optionally together with CRF and/or vasopressin) may stimulate native production of POMC and its regulatory peptides. A further aspect of the present invention therefore provides a method of stimulating POMC production in a patient, comprising administering exogenous POMC to the patient. The exogenous POMC is preferably non-human, and more preferably goat POMC.
Conveniently, administration is subcutaneous; this gives a subcutaneous depot of active composition for subsequent slow release into the patient's system.
A further aspect of the present invention provides a pharmaceutical composition comprising a POMC peptide.
According to a further aspect of the invention, there is provided a pharmaceutical composition comprising two or more of alpha, beta, and gamma melanocyte stimulating hormone (MSH); adrenocorticotrophin (ACTH); beta and gamma lipotropin (LPH); and beta endorphin. Given the likely proteolysis of POMC on administration, it may be possible to achieve similar effects by administration of two or more of the individual hormones derived from POMC.
The pharmaceutical composition may provide the recited hormones as individual peptides, or as one or more precursor molecules (for example, partial breakdown products of POMC). Preferably three, four, five, six, or seven of the hormones are included in the pharmaceutical composition which (optionally together with CRF) induce a cascade for continued production of such molecules. The various components may be provided in combination with one or more carrier molecules which bind one or more of the components, and so act as a depot or reservoir for release of the component. A carrier molecule may also be used in combination with POMC and its related peptides.
According to a further aspect of the present invention, there is provided a method of treatment for a disease selected from multiple sclerosis; rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating; inflammatory conditions; obesity;
nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering CRF to a patient in need thereof. Alternatively, the method may comprise administering POMC.
The optimal dosage of the treatment has not yet been determined; however it may be appropriate to administer the treatment in a dosage of between 0.01 and 10 mg/kg to the subject; more preferably between 0.01 and 5 mg/kg, between 0.025 and 2 mg/kg, and most preferably between 0.05 and 1 mg/kg.
In preliminary studies, a serum product has been administered to patients with a total protein concentration of 4 mg/mi.
The precise dosage to be administered may be varied depending on such factors as the age, sex and weight of the patient, the method and formulation of administration, as well as the nature and severity of the disorder to be treated. Other factors such as diet, time of administration, condition of the patient, drug combinations, and reaction sensitivity may be taken into account.
In addition to the active ingredients, such compositions may comprise suitable pharmaceutically acceptable carriers comprising excipients and other components which facilitate processing of the active compounds into preparations suitable for pharmaceutical administration.
Pharmaceutical compositions for oral administration can be formulated using pharmaceutically acceptable carriers known in the art in dosages suitable for oral administration. Such carriers enable the compositions to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions, and the like suitable for ingestion by the subject.
Pharmaceutical preparations for oral use can be obtained through combination of active compounds with a solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable additional compounds if desired to obtain tablets or dragee cores. Suitable excipients include carbohydrate or protein fillers such as sugars, including lactose, sucrose, mannitol, sorbitol; starch from corn, wheat, rice, potato, or other plants; cellulose such as methylcelfulose, hydroxypropylmethylcellulose, or sodium carboxymethylcel[u[ose; and gums including arabic and tragacanth;
as well as proteins such as gelatin and coliagen. If desired, disintegrating or solubilising agents may be added, such as cross linked polyvinyl pyrrolidone, agar, alginic acid, or a salt thereof.
Dragee cores can be provided with suitable coatings such as concentrated sugar solutions, which may also contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for product identification or to characterise the quantity of active compound.
Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a coating such as glycerol or sorbitol. Push-fit capsules can contain active ingredients mixed with a 1=liler or binders such as lactose or starches, lubricants such as talc or magnesium stearate, and, optionally stabilisers. In soft capsules, the active compounds can be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycol with or without stabilisers.
Pharmaceutical formulations for parenteral administration include aqueous solutions of active compounds. For injection, the pharmaceutical compositions of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiologically buffered saline. Aqueous suspension injections can contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Additionally, suspensions of the active compounds can be prepared as appropriate oily injection suspensions.
Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes.
Optionally, the suspension can also contain suitable stabilisers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.
For topical or nasal administration, penetrants appropriate to the particular barrier to be permeated may be used in the formulation.
The pharmaceutical compositions of the present invention can be manufactured substantially in accordance with standard manufacturing procedures known in the art.
The composition may also comprise one or more peptide regulatory or releasing factors, which may induce a cascade of release of further peptides by a variety of cells in the patient. Such additional factors are preferably derived from the 5 same source as the CRF, in particular goat serum. Suitable factors include aW
HLA, TGF-p, and IL-10, among others.
In preferred embodiments, the composition may comprise one or more of vasopressin, beta endorphin, and an enkephalin. In certain embodiments, the 10 composition may comprise CRF binding protein, CRF-BP. This binds CRF and may act as a reservoir for subsequent release of CRF to the patient.
The composition may further comprise a POMC peptide or a POMC product;
certain POMC products may be useful to administer to a patient to stimulate further production, or to obtain a desired response before endogenous POMC
can be produced.
Human POMC is described in detail in entry 176830 of OMIM (online mendelian inheritance in man, accessible through http=//www.ncbi.nlm.nih.gov/). The nucleotide and amino acid sequence of human POMC is also known, and has GENBANK accession number BC065832. Human POMC gives rise to a glycosylated protein precursor having a molecular weight of 31 kDa.
By "a POMC peptide" is meant any peptide having a corresponding sequence, structure, or function. It will be apparent to the skilled person that the canonical nucleotide and/or amino acid sequences given for human POMC in the GENBANK entry referenced above may be varied to a certain degree without affecting the structure or function of the peptide. In particular, allelic variants and functional mutants are included within this definition. Mutants may include conservative amino acid substitutions. "A POMC peptide" as used herein refers to any peptide acting as a precursor to at least one form of MSH, ACTH, at least one form of LPH, p endorphin, met-enkephalin and leu-enkephalin; and preferably all of a, p, and y MSH; ACTH; p and y LPH; and p endorphin, met-enkephalin and leu-enkephalin Although research has been carried out into the pharmaceutical potential of certain of the individual products of the POMC peptide, it is believed that administration of POMC itself has not been determined to have any medical use. It is possible that POMC itself is inactive, and must be cleaved into its products before having an activity.
Preferably the pharmaceutical composition comprises non-human POMC;
conveniently ungulate POMC; and most preferably goat POMC. Although POMC
is produced in the pituitary gland, and so would not be expected to be present in serum, at least at signihcant levels, it has been surprisingly identified that goat serum contains POMC, POMC-related peptides, and molecules associated with the POMC cascade, particularly when the goat is stimulated by physiological stress, such as bleeding or immunization. This provides a convenient source for POMC for pharmaceutical compositions of the present invention. It is also believed that POMC may have a self-sustaining effect in the patient, in that administration of an initial amount of POMC leads to endogenous production of POMC in the patient; thus, an initial administration of a low level of POMC may have a significant effect on the patient.
It is believed that, on administration of POMC and its associated molecules to a subject, the peptide is proteolysed to provide one or more of the products of POMC in a readily available form to the subject; there is also the induction of a molecular cascade which stimulates the hypotha[amo-pituitary-adrenal axis (HPA). This would be consistent with previously observed effects of the unpurihed goat serum; for example, a rapid 'buzz' effect on sublingual administration may be due to proteolysis of POMC to release p endorphin, which can then be absorbed through the mucous membranes. In addition, alpha MSH is known to have an effect on TL-10 and TGF-p production, which results in an anti-inflammatory effect, consistent with that which has been observed with goat serum. Alpha MSH is also known to inhibit the release of pro-inflammatory cytokines.
The composition may also have anti-inflammatory properties. We believe there is a passive transfer of anti-inflammatory response from the goat or other animal used to produce the serum to the patient. This is a consequence of the puriflcation process used to prepare the composition, in which a variety of active factors are retained in the serum. The composition may also comprise additional active components which provide an anti-inflammatory effect.
As mentioned above, it is believed that an initial administration of POMC
(optionally together with CRF and/or vasopressin) may stimulate native production of POMC and its regulatory peptides. A further aspect of the present invention therefore provides a method of stimulating POMC production in a patient, comprising administering exogenous POMC to the patient. The exogenous POMC is preferably non-human, and more preferably goat POMC.
Conveniently, administration is subcutaneous; this gives a subcutaneous depot of active composition for subsequent slow release into the patient's system.
A further aspect of the present invention provides a pharmaceutical composition comprising a POMC peptide.
According to a further aspect of the invention, there is provided a pharmaceutical composition comprising two or more of alpha, beta, and gamma melanocyte stimulating hormone (MSH); adrenocorticotrophin (ACTH); beta and gamma lipotropin (LPH); and beta endorphin. Given the likely proteolysis of POMC on administration, it may be possible to achieve similar effects by administration of two or more of the individual hormones derived from POMC.
The pharmaceutical composition may provide the recited hormones as individual peptides, or as one or more precursor molecules (for example, partial breakdown products of POMC). Preferably three, four, five, six, or seven of the hormones are included in the pharmaceutical composition which (optionally together with CRF) induce a cascade for continued production of such molecules. The various components may be provided in combination with one or more carrier molecules which bind one or more of the components, and so act as a depot or reservoir for release of the component. A carrier molecule may also be used in combination with POMC and its related peptides.
According to a further aspect of the present invention, there is provided a method of treatment for a disease selected from multiple sclerosis; rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating; inflammatory conditions; obesity;
nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering CRF to a patient in need thereof. Alternatively, the method may comprise administering POMC.
The optimal dosage of the treatment has not yet been determined; however it may be appropriate to administer the treatment in a dosage of between 0.01 and 10 mg/kg to the subject; more preferably between 0.01 and 5 mg/kg, between 0.025 and 2 mg/kg, and most preferably between 0.05 and 1 mg/kg.
In preliminary studies, a serum product has been administered to patients with a total protein concentration of 4 mg/mi.
The precise dosage to be administered may be varied depending on such factors as the age, sex and weight of the patient, the method and formulation of administration, as well as the nature and severity of the disorder to be treated. Other factors such as diet, time of administration, condition of the patient, drug combinations, and reaction sensitivity may be taken into account.
An effective treatment regimen may be determined by the clinician responsible for the treatment. One or more administrations may be given, and typically the benefits are observed after a series of at least three, five, or more administrations. Repeated administration may be desirable to maintain the beneficial effects of the composition.
The treatment may be administered by any effective route, preferably by subcutaneous injection, although alternative routes which may be used include intramuscular or intralesional injection, oral, aerosol, parenteral, or topical.
The treatment is preferably administered as a liquid formulation, although other formulations may be used. For example, the treatment may be mixed with suitable pharmaceutically acceptable carriers, and may be formulated as solids (tablets, pills, capsules, granules, etc) in a suitable composition for oral, topical 1s or parenteral administration.
The invention also provides the use of CRF in the preparation of a medicament for the treatment of one or more of the diseases recited above. Also provided is the use of POMC in the preparation of a medicament for the treatment of one or more of the diseases recited above. The CRF or the POMC may be isolated, purifled CRF or POMC, although it is preferred that they are administered in combination with the various other components as discussed above. In particular, bioactive carrier proteins and vasopressin may be used.
According to a further aspect of the present invention, there is provided a method of producing CRF, the method comprising the steps of obtaining a blood sample from a goat; separating the serum from the remaining blood components; and purifying the serum by precipitation of solids.
The precipitate may further be resuspended in a physiologically acceptable buffer; for example, PBS buffer. The resuspended precipitate may further be purified by dialysis or diai=litration; for example, with a molecular weight cut-off of 50,000 Da, preferably 40,000 Da, and more preferably 31,000 Da.
The precipitate may undergo further purification to isolate CRF; for example, 5 antibody or other affinity puriflcation. CRF may be bound by antibodies raised against CRF, or by use of CRF-BP.
Separation of the serum may be achieved by centrifugation.
10 The serum may further be purihed by viral flitration; it is preferred that any purii=lcation method used does not inactivate or remove any of the bioactive components of the serum, which may include components other than CRF /
POMC.
The treatment may be administered by any effective route, preferably by subcutaneous injection, although alternative routes which may be used include intramuscular or intralesional injection, oral, aerosol, parenteral, or topical.
The treatment is preferably administered as a liquid formulation, although other formulations may be used. For example, the treatment may be mixed with suitable pharmaceutically acceptable carriers, and may be formulated as solids (tablets, pills, capsules, granules, etc) in a suitable composition for oral, topical 1s or parenteral administration.
The invention also provides the use of CRF in the preparation of a medicament for the treatment of one or more of the diseases recited above. Also provided is the use of POMC in the preparation of a medicament for the treatment of one or more of the diseases recited above. The CRF or the POMC may be isolated, purifled CRF or POMC, although it is preferred that they are administered in combination with the various other components as discussed above. In particular, bioactive carrier proteins and vasopressin may be used.
According to a further aspect of the present invention, there is provided a method of producing CRF, the method comprising the steps of obtaining a blood sample from a goat; separating the serum from the remaining blood components; and purifying the serum by precipitation of solids.
The precipitate may further be resuspended in a physiologically acceptable buffer; for example, PBS buffer. The resuspended precipitate may further be purified by dialysis or diai=litration; for example, with a molecular weight cut-off of 50,000 Da, preferably 40,000 Da, and more preferably 31,000 Da.
The precipitate may undergo further purification to isolate CRF; for example, 5 antibody or other affinity puriflcation. CRF may be bound by antibodies raised against CRF, or by use of CRF-BP.
Separation of the serum may be achieved by centrifugation.
10 The serum may further be purihed by viral flitration; it is preferred that any purii=lcation method used does not inactivate or remove any of the bioactive components of the serum, which may include components other than CRF /
POMC.
15 Precipitation may be carried out by ammonium sulphate precipitation, or by caprylic acid purification. Other suitable precipitating agents may be used.
Preferably the goat is an immunized goat. It is believed that immunization of the goat stimulates the production of CRF and vasopressin, such that it is present in the serum in higher levels. The method may also comprise the step of immunizing a goat. Alternatively, the goat may be subject to physiological stress, for example, bleeding.
It is also believed that, in some circumstances, the use of an immunogen may not be necessary, and that useful product may be obtained from a non-immunised animal (that is, one which has not been pre-immunised with a specific immunogen). It is accepted that a normal goat may well have been previously exposed to environmental immunogens, and such goats may be used in preparation of the compositions of the present invention. The present invention is therefore intended also to encompass pharmaceutical compositions comprising serum obtained from a non-immunised goat. The invention also extends to uses of such compositions or such serum in the treatment of, or in the preparation of medicaments for the treatment of, the various diseases or disorders recited above in the section headed "uses of the serum composition".
The invention still further extends to the preparation of POMC and/or CRF from serum obtained from a non-immunised goat.
BRIEF DESCRIPTION OF THE DRAWINGS
These and other aspects of the present invention will now be described by way of example only with reference to the accompanying drawings, in which:
Figures I. to 4 show mass spectrometry analyses of tryptic digests of serum components;
Figures 5 to 7 show mass spectrometry analyses of patient sera before and after treatment with the composition;
Figures 8 and 9 show analyses of induction of POMC peptides by treatment with the composition;
Figures 10 to 14 show evidence for a switch in inflammatory profile of patients following treatment with the composition;
Figure 15 shows levels of vasopressin in human serum and the composition;
Figure 16 shows levels of CRF in human serum and the composition; and Figure 17 shows a summary diagram of the proposed elements of the composition and their method of action.
DETAILED DESCRIPTION OF THE INVENTION
Preparation of serum composition Approximately 400 cc of blood is taken from a goat under sterile technique.
The animal may typically be re-bled in 10 to 14 days, once the volume of blood is replenished. A pre-bleeding regime may be useful to stimulate production of the active components of the serum. The blood is then centrifuged to separate the serum, and the serum filtered to remove large clots and particulate matter.
The serum is then treated with supersaturated ammonium sulphate (47% solution at 4 C) to precipitate antibodies and other material. The resulting solution is centrifuged in a Beckman J6M/E centrifuge at 3500 rpm for 45 minutes, after which the supernatant fluid is removed. The precipitated immunoglobulin and other solid material are resuspended in PBS buffer (phosphate buffered saline) sufticient to redissolve the precipitate.
The solution is then subjected to diafiltration against a PBS buffer with a 1o molecular weight cut-off of 10,000 Daltons. at 4 C. After diafiltration the product is flltered through a 0.2 micron filter into a sterile container and adjusted to a protein concentration of 4mg/ml. The solution is put into vials to give single doses of lml, and stored at -22 C prior to use.
Discussion The effects of the serum have been previously described, while determination of the active components has not previously been effected.
Analysis of serum composition A sample of the composition was size fractionated on a gel, and a Western blot performed using antibodies to p endorphin. A strong signal was detected, indicating the presence of p endorphin, although the apparent molecular weight was approximately 31 kDa, far larger than the expected size of (3 endorphin.
This suggested that p endorphin was present in the sample as part of a larger peptide; the size being consistent with that of POMC.
We have also carried out mass spectrometry on the composition, and have detected at least two POMC-derived peptides, p endorphin and corticotrophin-related molecules. CRH-BP (corticotropin releasing hormone binding protein) has also been identified.
Preferably the goat is an immunized goat. It is believed that immunization of the goat stimulates the production of CRF and vasopressin, such that it is present in the serum in higher levels. The method may also comprise the step of immunizing a goat. Alternatively, the goat may be subject to physiological stress, for example, bleeding.
It is also believed that, in some circumstances, the use of an immunogen may not be necessary, and that useful product may be obtained from a non-immunised animal (that is, one which has not been pre-immunised with a specific immunogen). It is accepted that a normal goat may well have been previously exposed to environmental immunogens, and such goats may be used in preparation of the compositions of the present invention. The present invention is therefore intended also to encompass pharmaceutical compositions comprising serum obtained from a non-immunised goat. The invention also extends to uses of such compositions or such serum in the treatment of, or in the preparation of medicaments for the treatment of, the various diseases or disorders recited above in the section headed "uses of the serum composition".
The invention still further extends to the preparation of POMC and/or CRF from serum obtained from a non-immunised goat.
BRIEF DESCRIPTION OF THE DRAWINGS
These and other aspects of the present invention will now be described by way of example only with reference to the accompanying drawings, in which:
Figures I. to 4 show mass spectrometry analyses of tryptic digests of serum components;
Figures 5 to 7 show mass spectrometry analyses of patient sera before and after treatment with the composition;
Figures 8 and 9 show analyses of induction of POMC peptides by treatment with the composition;
Figures 10 to 14 show evidence for a switch in inflammatory profile of patients following treatment with the composition;
Figure 15 shows levels of vasopressin in human serum and the composition;
Figure 16 shows levels of CRF in human serum and the composition; and Figure 17 shows a summary diagram of the proposed elements of the composition and their method of action.
DETAILED DESCRIPTION OF THE INVENTION
Preparation of serum composition Approximately 400 cc of blood is taken from a goat under sterile technique.
The animal may typically be re-bled in 10 to 14 days, once the volume of blood is replenished. A pre-bleeding regime may be useful to stimulate production of the active components of the serum. The blood is then centrifuged to separate the serum, and the serum filtered to remove large clots and particulate matter.
The serum is then treated with supersaturated ammonium sulphate (47% solution at 4 C) to precipitate antibodies and other material. The resulting solution is centrifuged in a Beckman J6M/E centrifuge at 3500 rpm for 45 minutes, after which the supernatant fluid is removed. The precipitated immunoglobulin and other solid material are resuspended in PBS buffer (phosphate buffered saline) sufticient to redissolve the precipitate.
The solution is then subjected to diafiltration against a PBS buffer with a 1o molecular weight cut-off of 10,000 Daltons. at 4 C. After diafiltration the product is flltered through a 0.2 micron filter into a sterile container and adjusted to a protein concentration of 4mg/ml. The solution is put into vials to give single doses of lml, and stored at -22 C prior to use.
Discussion The effects of the serum have been previously described, while determination of the active components has not previously been effected.
Analysis of serum composition A sample of the composition was size fractionated on a gel, and a Western blot performed using antibodies to p endorphin. A strong signal was detected, indicating the presence of p endorphin, although the apparent molecular weight was approximately 31 kDa, far larger than the expected size of (3 endorphin.
This suggested that p endorphin was present in the sample as part of a larger peptide; the size being consistent with that of POMC.
We have also carried out mass spectrometry on the composition, and have detected at least two POMC-derived peptides, p endorphin and corticotrophin-related molecules. CRH-BP (corticotropin releasing hormone binding protein) has also been identified.
Fiaures 1 to 4 POMC peptides and CRF-BP have been identii=led in the product by Thermofinnegan LCQ mass spectrometry. CRF mainly regulates the synthesis and secretion of ACTH in the anterior pituitary. The administration of POMC
and/or its component peptides in addition to CRF and CRF-BP is thought to initiate a cascade effect thus enhancing the production of systemic and sustained elevated concentrations of POMC peptides. CRF-BP has the ability to act as a reservoir for CRF.
Figures I to 4 show the hits obtained from mass spectrometry analysis of tryptic digests from the product separated from contaminating proteins by SDS-PAGE. As mentioned above, some of these molecules are inducers and regulators of the POMC cascade. Further investigation using more focused analysis (e.g. peptide fractionation, immunoprecipitation and concentration) will reveal more of the peptides present. Figure 1 indicates the presence of a POMC-derived corticotropin, Figure 2 that of CRF-BP, Figure 3 that of proenkephalin A, and Figure 4 that of proenkephalin B. The presence of CRF-BP
suggests that the product contains some CRF, while POMC and related peptides are also clearly present.
We have also investigated the effects of treatment with the serum composition on patients' own sera. These effects are described below.
Treatment induces protein/peptide expression in patients' sera Figure 5 shows mass spectrometry of patients' sera before and after treatment.
The spectra from 2 to 10 kD are compared. This molecular weight range is associated with the bioactive peptides of interest. Clear differences in the peptide expression in the 2 to 6 kD region can be seen by comparing the profiles in the pre and post treatment sera. For ease of comparison an overlapping view of the profiies is also provided.
and/or its component peptides in addition to CRF and CRF-BP is thought to initiate a cascade effect thus enhancing the production of systemic and sustained elevated concentrations of POMC peptides. CRF-BP has the ability to act as a reservoir for CRF.
Figures I to 4 show the hits obtained from mass spectrometry analysis of tryptic digests from the product separated from contaminating proteins by SDS-PAGE. As mentioned above, some of these molecules are inducers and regulators of the POMC cascade. Further investigation using more focused analysis (e.g. peptide fractionation, immunoprecipitation and concentration) will reveal more of the peptides present. Figure 1 indicates the presence of a POMC-derived corticotropin, Figure 2 that of CRF-BP, Figure 3 that of proenkephalin A, and Figure 4 that of proenkephalin B. The presence of CRF-BP
suggests that the product contains some CRF, while POMC and related peptides are also clearly present.
We have also investigated the effects of treatment with the serum composition on patients' own sera. These effects are described below.
Treatment induces protein/peptide expression in patients' sera Figure 5 shows mass spectrometry of patients' sera before and after treatment.
The spectra from 2 to 10 kD are compared. This molecular weight range is associated with the bioactive peptides of interest. Clear differences in the peptide expression in the 2 to 6 kD region can be seen by comparing the profiles in the pre and post treatment sera. For ease of comparison an overlapping view of the profiies is also provided.
Figure 6 shows comparative peptide / protein expression in six treated patients.
Each patient shows increased levels of induced peptide / protein expression particularly in the 4 kD region.
Figure 7a shows the mass spectrometry profiles of unprocessed goat serum before vaccination (pre-immune profile, top panel), unprocessed serum 53 days post-immunisation, and the processed product. It can be seen that in the lower two panels the profile of the serum is significantly different to that of the pre-immune profile, indicative of the induction of protein expression. The profiles present here represent the active product, and a specific immunisation / bleed protocol has been shown to be useful in the induction of this serum profile.
An overlapping view of the profiles is shown (Figure 7b).
Evidence for the induction of PDMC e tides Figure 8 shows comparative levels of ACTH in the sera of patients before and after receiving treatment. This is also compared with levels of ACTH in serum from healthy volunteers and in the product administered to patients. Sera were diluted 1:100 and quantihed by an ELISA of sera compared with the product.
Data are the mean of three determinations +/- standard errors. Post treatment n=5; pre treatment n=3; normal human sera n-5. The data show that treatment increases ACTH levels.
Figure 9 compares levels of R endorphin in the serum of treated patients with that in the sera of the same patients before treatment. This is compared with levels in the sera of healthy volunteers and in the product. Sera were diluted 1:100 and quantified by an ELISA of sera compared with the product. Data are the mean of three determinations +/- standard errors. The data show that treatment increases p endorphin levels.
Evidence for a switch from a pro-inflammatory TH-1 profile to an anti-inflammatory TH-2 c~,tokine profile in treated patients Figure 10 shows the levels of TGi=-P in the serum of two groups of patients before and after treatment. The two groups of patients (n=3 for each group) 5 show differing responses with respect to the concentrations of TGF-D
produced, but all patients showed an increase in serum levels in response to treatment (pre sera = patients' serum levels before treatment; post 2nd and post 5th =
after the 2nd and 5th administration). The data show that treatment induces increased concentration of the anti-inflammatory cytokine TGF-p.
Figure 11 shows the levels of IL-4 in the serum of one group of patients before (pre-sera) and after treatment. It can be seen that after treatment (post znd), the levels of IL-4 are signil=lcantly increased in the patients' sera (n=5).
However, following the 5th administration, the levels of IL-4 had dropped in all patients, but remained higher than they had been pre-treatment. IL-4 is known to downregulate the production of the pro-inflammatory cytokines from TH-1 cells. It may be that the consistent changes in concentration seen in all patients is consistent with IL-4's role in the TH-1 to TH-2 switch.
Figure 12 shows the levels of IL-6 in the serum of one group of patients before and after treatment. It can be seen that after treatment (post 2nd and post 5 th) the levels of IL-6 are reduced in the patients' sera (n=4).
Figure 13 shows the levels of IFN-y in the serum of one group of patients before and after treatment. It can be seen that after treatment (post 2nd and post 5t") the levels of IFN-y are reduced in the patients' sera.
Figure 14 shows that treatment of human peripheral blood cells (PBMCs) induces the production of the anti-inflammatory cytokine IL-iQ in the monocyte sub population. T and B lymphocytes and monocytes were separated from PBMCs obtained from human volunteers. All cell types were treated with equivalent doses of product for 16h, and their supernatants assayed for IL-10 content using ELISA. It can be seen that IL.-10 levels produced by the T cell population were unaffected by treatment and that only a small increase in IL-was induced in the B cells. However, a significant elevation of IL-10 concentration was induced in the monocytes population by the treatment. All determinations were made in triplicate +/- standard deviations. These data are representative of at least three separate experiments.
Evidence for vasopressin and CRF induction Figure 15 shows the comparative levels of vasopressin in the product, control patients and patients treated with the product and pre-treatment. The figure shows that there is no significant difference between any of the serum groups, however the product contains significant levels of vasopressin, sufficient to elicit a response in the patients. It is known that vasopressin acts synergistically with CRF to release POMC. All determinations were made in triplicate +/- standard deviations. These data are representative of at least 3 separate experiments.
Patients pre-treatment n=3; treated patients n= 6.
Figure 16 shows the increased presence of CRF in the product compared with the placebo and the increase in the treated patients compared with the non-treated individuals; the latter is evidence for the induction of CRF in the patients in response to treatment. All determinations were made in triplicate +/- standard deviations. These data are representative of at least 3 separate experiments. Control individuals n=4; treated patients n= 13.
Summary and conclusions Although preliminary, the evidence to date is therefore consistent with the major active component being CRF acting in concert with other components, which is thought to induce POMC production. There is also evidence that POMC
itself and POMC-derived peptides may be used as a treatment. This suggests new pharmaceutical compositions and uses for CRF and POMC, as well as indicating additional disorders which may be treatable using CRF and POMC.
We have also provided a convenient method of producing CRF and PDMC from goats.
The data so far suggests that the product not only contains CRF, POMC
peptides and anti-inflammatory cytokines (iL-10 and TGF-(3) but also induces the expression and release of CRF and hence POMC peptidds in the patient, which then transform the patients' immunological profile from a TH-1 pro-inflammatory profle to a predominantly TH-2 anti-inflammatory profile.
Other observations on the composition effects are consistent with the active component being CRF which leads to POMC production. For example, effects on leukocyte adherence may be attributable to beta endorphin. The serum product increases IL-10 production by human PBMC; alpha MSH affects IL-10 production. Effects on nerve conduction and neuroprotective effects may be ascribed to ACTH and vasopressin; effects on appetite may be due to alpha MSH. The product itself also contains significant levels of IL-10 and TGF-p (data not shown).
Alpha MSH has potent anti-inflammatory effects in all major forms of inflammation and it antagonises the effects of pro-inflammatory cytokines such as TNFa and ILi-(3. Cross talk exists between the cytokine systems and the POMC system which has been observed in patients treated with the composition to result in the reduction of pro-inflammatory cytokines and the establishment (retained over the course of treatment) of a TH-2 anti-inflammatory cytokine profile including elevated levels of IL-Ip and TGF-p. We have also identified increased levels of ILI.-P in the serum product.
The serum product has previously been shown to be very sensitive to proteolytic degradation; this is consistent with the theory that the POMC is proteolysed to give individual hormones on administration, but that further degradation destroys activity. In particular, alpha MSH is believed to have significantly reduced activity if a terminal tripeptide sequence is removed;
again, this is consistent with the active component including POMC. The product itself is unstable by nature as its active components are short-lived, but exhibit powerful effects.
We have also conducted experiments which suggest that the serum modulates nitric oxide production by leukocytes; this is consistent with effects of beta endorphin. We also believe that the serum inhibits PHA-induced PBMC
proliferation, suggesting an explanation for the serum's immunomodulatory effects. We have also seen a reduced response of PBMCs in the presence of the product to LPS-induced stimulation and mixed lymphocyte reactions (data not shown).
The product may also induce tyrosine phosphorylation in human brain microglial cells, and has been shown by Western blotting to modulate the NFicB pathway (data not shown). NFxB is known to regulate the transcription of genes involved in the regulation of pro-inflammatory cytokines, hence the inhibition of NFicB would act to reduce the pro-inflammatory cytokine response in autoimmune disease and reduce inflammatory responses. Further experiments to investigate this are underway.
Receptors (MCR3 and MCR4) for some POMC peptides are found in the retinal ganglion cells that form the optic nerve and may be stimulated by POMC
peptides produced after treatment. This may account for some of the rapid improvements in vision experienced by MS patients with optic neuritis which have previously been described. It is known that ACTH triggers the corticosteroid pathway which can exert effects in as little as 20 to 30 minutes.
Preliminary data suggests that the concentrations of the peptides in the product may be insufficient to elicit therapeutic responses in patients after dilution in the blood volume of the patient. However, the product could act locally (as it is injected in a subcutaneous bolus) to induce a biochemical cascade which triggers the synthesis and release of the bioactive peptides in the treated patients. It is now known that any medical treatments that interfere with the product, for example by competing for receptors or blocking molecules in the HPA should be avoided.
In support of this hypothesis mass spectrometry of the product has identified additional molecules some of which are involved in the induction and regulation of the corticotropin system; namely CRH binding protein and leu-enkephalin, corticotropin-lipotropin precursor and pro-enkephalin A precursors (see Figures I to 4). In addition, and perhaps more importantly, we have discovered that two of the major POMC peptides are upregulated significantly in treated patients' sera compared with levels before treatment, and also compared with levels from healthy control volunteers. This finding, together with immunological data, suggests that the treatment induces the expression and release of POMC peptides in the patient, which then transforms the patients' immunological profile from a TH-1 pro-inflammatory proflfe to a TH-2 anti-inflammatory pro1=<le. The further elucidation of the cascade mechanism in the patients is currently under investigation.
It should be noted that although the product is anti-inflammatory in nature it does not completely inhibit the inflammatory response. Our data suggest that the product induces a shift from the unfavourable TH-1 cytokine profile seen in auto-immune diseases to a more favourable balanced cytokine level. This may appear initially after treatment as a rapid anti-inflammatory TH-2 shift as the TH-1 network is turned off. Later on after treatment the TH-1 network operates albeit at a lower level.
The reported effects of the serum product on tumours leads us to consider the possibility of anti-angiogenic effects of the serum. In this regard, the proteins thrombospondin-1 (TSP-1) and platelet factor 4 (PF-4) have been identifled in the product by mass spectrometry of tryptic digests from SDS PAGE gels.
5 Computer database searches using Biowork Browser for peptide identification yielded strong matches across several species including Homo sapiens.
Although precise quantification of the TSP-1 and PF-4 protein content of the product has not yet been established, the visible nature of the protein bands on SDS PAGE gels indicates that the proteins are present in biologically significant 10 (upper nanogram) quantities.
A summary of the hypothesised components of the product, and the method of action, is shown in Figure 17. The product is thought to contain CRF, with some levels of CRF-BP, beta endorphin, vasopressin, and enkephalins. CRF induces 15 production of further CRF in the patient, as do beta endorphin and the enkephalins. Endogenous CRF causes production of POMC, which gives rise to among others ACTH, alpha MSH, and beta endorphin. This last product acts in a feedback loop, with low levels stimulating further CRF release, while high levels inhibit CRF release. This whole CRF / POMC cascade is thought to induce an 20 immunological switch in the patient, which could explain the surprising beneficial effects seen in a variety of conditions.
Each patient shows increased levels of induced peptide / protein expression particularly in the 4 kD region.
Figure 7a shows the mass spectrometry profiles of unprocessed goat serum before vaccination (pre-immune profile, top panel), unprocessed serum 53 days post-immunisation, and the processed product. It can be seen that in the lower two panels the profile of the serum is significantly different to that of the pre-immune profile, indicative of the induction of protein expression. The profiles present here represent the active product, and a specific immunisation / bleed protocol has been shown to be useful in the induction of this serum profile.
An overlapping view of the profiles is shown (Figure 7b).
Evidence for the induction of PDMC e tides Figure 8 shows comparative levels of ACTH in the sera of patients before and after receiving treatment. This is also compared with levels of ACTH in serum from healthy volunteers and in the product administered to patients. Sera were diluted 1:100 and quantihed by an ELISA of sera compared with the product.
Data are the mean of three determinations +/- standard errors. Post treatment n=5; pre treatment n=3; normal human sera n-5. The data show that treatment increases ACTH levels.
Figure 9 compares levels of R endorphin in the serum of treated patients with that in the sera of the same patients before treatment. This is compared with levels in the sera of healthy volunteers and in the product. Sera were diluted 1:100 and quantified by an ELISA of sera compared with the product. Data are the mean of three determinations +/- standard errors. The data show that treatment increases p endorphin levels.
Evidence for a switch from a pro-inflammatory TH-1 profile to an anti-inflammatory TH-2 c~,tokine profile in treated patients Figure 10 shows the levels of TGi=-P in the serum of two groups of patients before and after treatment. The two groups of patients (n=3 for each group) 5 show differing responses with respect to the concentrations of TGF-D
produced, but all patients showed an increase in serum levels in response to treatment (pre sera = patients' serum levels before treatment; post 2nd and post 5th =
after the 2nd and 5th administration). The data show that treatment induces increased concentration of the anti-inflammatory cytokine TGF-p.
Figure 11 shows the levels of IL-4 in the serum of one group of patients before (pre-sera) and after treatment. It can be seen that after treatment (post znd), the levels of IL-4 are signil=lcantly increased in the patients' sera (n=5).
However, following the 5th administration, the levels of IL-4 had dropped in all patients, but remained higher than they had been pre-treatment. IL-4 is known to downregulate the production of the pro-inflammatory cytokines from TH-1 cells. It may be that the consistent changes in concentration seen in all patients is consistent with IL-4's role in the TH-1 to TH-2 switch.
Figure 12 shows the levels of IL-6 in the serum of one group of patients before and after treatment. It can be seen that after treatment (post 2nd and post 5 th) the levels of IL-6 are reduced in the patients' sera (n=4).
Figure 13 shows the levels of IFN-y in the serum of one group of patients before and after treatment. It can be seen that after treatment (post 2nd and post 5t") the levels of IFN-y are reduced in the patients' sera.
Figure 14 shows that treatment of human peripheral blood cells (PBMCs) induces the production of the anti-inflammatory cytokine IL-iQ in the monocyte sub population. T and B lymphocytes and monocytes were separated from PBMCs obtained from human volunteers. All cell types were treated with equivalent doses of product for 16h, and their supernatants assayed for IL-10 content using ELISA. It can be seen that IL.-10 levels produced by the T cell population were unaffected by treatment and that only a small increase in IL-was induced in the B cells. However, a significant elevation of IL-10 concentration was induced in the monocytes population by the treatment. All determinations were made in triplicate +/- standard deviations. These data are representative of at least three separate experiments.
Evidence for vasopressin and CRF induction Figure 15 shows the comparative levels of vasopressin in the product, control patients and patients treated with the product and pre-treatment. The figure shows that there is no significant difference between any of the serum groups, however the product contains significant levels of vasopressin, sufficient to elicit a response in the patients. It is known that vasopressin acts synergistically with CRF to release POMC. All determinations were made in triplicate +/- standard deviations. These data are representative of at least 3 separate experiments.
Patients pre-treatment n=3; treated patients n= 6.
Figure 16 shows the increased presence of CRF in the product compared with the placebo and the increase in the treated patients compared with the non-treated individuals; the latter is evidence for the induction of CRF in the patients in response to treatment. All determinations were made in triplicate +/- standard deviations. These data are representative of at least 3 separate experiments. Control individuals n=4; treated patients n= 13.
Summary and conclusions Although preliminary, the evidence to date is therefore consistent with the major active component being CRF acting in concert with other components, which is thought to induce POMC production. There is also evidence that POMC
itself and POMC-derived peptides may be used as a treatment. This suggests new pharmaceutical compositions and uses for CRF and POMC, as well as indicating additional disorders which may be treatable using CRF and POMC.
We have also provided a convenient method of producing CRF and PDMC from goats.
The data so far suggests that the product not only contains CRF, POMC
peptides and anti-inflammatory cytokines (iL-10 and TGF-(3) but also induces the expression and release of CRF and hence POMC peptidds in the patient, which then transform the patients' immunological profile from a TH-1 pro-inflammatory profle to a predominantly TH-2 anti-inflammatory profile.
Other observations on the composition effects are consistent with the active component being CRF which leads to POMC production. For example, effects on leukocyte adherence may be attributable to beta endorphin. The serum product increases IL-10 production by human PBMC; alpha MSH affects IL-10 production. Effects on nerve conduction and neuroprotective effects may be ascribed to ACTH and vasopressin; effects on appetite may be due to alpha MSH. The product itself also contains significant levels of IL-10 and TGF-p (data not shown).
Alpha MSH has potent anti-inflammatory effects in all major forms of inflammation and it antagonises the effects of pro-inflammatory cytokines such as TNFa and ILi-(3. Cross talk exists between the cytokine systems and the POMC system which has been observed in patients treated with the composition to result in the reduction of pro-inflammatory cytokines and the establishment (retained over the course of treatment) of a TH-2 anti-inflammatory cytokine profile including elevated levels of IL-Ip and TGF-p. We have also identified increased levels of ILI.-P in the serum product.
The serum product has previously been shown to be very sensitive to proteolytic degradation; this is consistent with the theory that the POMC is proteolysed to give individual hormones on administration, but that further degradation destroys activity. In particular, alpha MSH is believed to have significantly reduced activity if a terminal tripeptide sequence is removed;
again, this is consistent with the active component including POMC. The product itself is unstable by nature as its active components are short-lived, but exhibit powerful effects.
We have also conducted experiments which suggest that the serum modulates nitric oxide production by leukocytes; this is consistent with effects of beta endorphin. We also believe that the serum inhibits PHA-induced PBMC
proliferation, suggesting an explanation for the serum's immunomodulatory effects. We have also seen a reduced response of PBMCs in the presence of the product to LPS-induced stimulation and mixed lymphocyte reactions (data not shown).
The product may also induce tyrosine phosphorylation in human brain microglial cells, and has been shown by Western blotting to modulate the NFicB pathway (data not shown). NFxB is known to regulate the transcription of genes involved in the regulation of pro-inflammatory cytokines, hence the inhibition of NFicB would act to reduce the pro-inflammatory cytokine response in autoimmune disease and reduce inflammatory responses. Further experiments to investigate this are underway.
Receptors (MCR3 and MCR4) for some POMC peptides are found in the retinal ganglion cells that form the optic nerve and may be stimulated by POMC
peptides produced after treatment. This may account for some of the rapid improvements in vision experienced by MS patients with optic neuritis which have previously been described. It is known that ACTH triggers the corticosteroid pathway which can exert effects in as little as 20 to 30 minutes.
Preliminary data suggests that the concentrations of the peptides in the product may be insufficient to elicit therapeutic responses in patients after dilution in the blood volume of the patient. However, the product could act locally (as it is injected in a subcutaneous bolus) to induce a biochemical cascade which triggers the synthesis and release of the bioactive peptides in the treated patients. It is now known that any medical treatments that interfere with the product, for example by competing for receptors or blocking molecules in the HPA should be avoided.
In support of this hypothesis mass spectrometry of the product has identified additional molecules some of which are involved in the induction and regulation of the corticotropin system; namely CRH binding protein and leu-enkephalin, corticotropin-lipotropin precursor and pro-enkephalin A precursors (see Figures I to 4). In addition, and perhaps more importantly, we have discovered that two of the major POMC peptides are upregulated significantly in treated patients' sera compared with levels before treatment, and also compared with levels from healthy control volunteers. This finding, together with immunological data, suggests that the treatment induces the expression and release of POMC peptides in the patient, which then transforms the patients' immunological profile from a TH-1 pro-inflammatory proflfe to a TH-2 anti-inflammatory pro1=<le. The further elucidation of the cascade mechanism in the patients is currently under investigation.
It should be noted that although the product is anti-inflammatory in nature it does not completely inhibit the inflammatory response. Our data suggest that the product induces a shift from the unfavourable TH-1 cytokine profile seen in auto-immune diseases to a more favourable balanced cytokine level. This may appear initially after treatment as a rapid anti-inflammatory TH-2 shift as the TH-1 network is turned off. Later on after treatment the TH-1 network operates albeit at a lower level.
The reported effects of the serum product on tumours leads us to consider the possibility of anti-angiogenic effects of the serum. In this regard, the proteins thrombospondin-1 (TSP-1) and platelet factor 4 (PF-4) have been identifled in the product by mass spectrometry of tryptic digests from SDS PAGE gels.
5 Computer database searches using Biowork Browser for peptide identification yielded strong matches across several species including Homo sapiens.
Although precise quantification of the TSP-1 and PF-4 protein content of the product has not yet been established, the visible nature of the protein bands on SDS PAGE gels indicates that the proteins are present in biologically significant 10 (upper nanogram) quantities.
A summary of the hypothesised components of the product, and the method of action, is shown in Figure 17. The product is thought to contain CRF, with some levels of CRF-BP, beta endorphin, vasopressin, and enkephalins. CRF induces 15 production of further CRF in the patient, as do beta endorphin and the enkephalins. Endogenous CRF causes production of POMC, which gives rise to among others ACTH, alpha MSH, and beta endorphin. This last product acts in a feedback loop, with low levels stimulating further CRF release, while high levels inhibit CRF release. This whole CRF / POMC cascade is thought to induce an 20 immunological switch in the patient, which could explain the surprising beneficial effects seen in a variety of conditions.
Claims (17)
1. A pharmaceutical composition comprising a corticotropin releasing factor (CRF) peptide.
2.The composition of claim 1, comprising goat CRF.
3. The composition of claim 1 or 2, further comprising one or more of vasopressin, beta endorphin, and an enkephalin.
4. The composition of any preceding claim, comprising CRF binding protein.
5. The composition of any preceding claim further comprising a POMC peptide.
6. The composition of any preceding claim further comprising one or more of a, .beta., and .gamma. MSH; ACTH; .beta. and .gamma. LPH; met-enkephalin, leu-enkephalin and beta endorphin.
7. A method of stimulating POMC production in a patient, comprising administering exogenous POMC to the patient.
8. The method of claim 7, further comprising administering exogenous CRF to the patient.
9. The method of claim 7 or claim 8, further comprising administering exogenous vasopressin to the patient.
10. A pharmaceutical composition comprising a POMC peptide.
11. Use of an isolated CRF peptide in the preparation of a medicament.
12. Use of an isolated POMC peptide in the preparation of a medicament.
13. A method of treatment for a disease selected from multiple sclerosis;
rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
axonal or nerve damage; cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating;
inflammatory conditions; obesity; nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering CRF to a patient in need thereof.
rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
axonal or nerve damage; cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating;
inflammatory conditions; obesity; nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering CRF to a patient in need thereof.
14. A method of treatment for a disease selected from multiple sclerosis;
rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
axonal or nerve damage; cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating;
inflammatory conditions; obesity; nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering POMC to a patient in need thereof.
rheumatoid arthritis; optic neuritis; motor neurone disease; autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
axonal or nerve damage; cancers, in particular myelomas, melanomas, and lymphomas; neural disorders, both demyelinating and non-demyelinating;
inflammatory conditions; obesity; nerve conduction disorders; and sexual dysfunction, in particular erectile dysfunction; the method comprising administering POMC to a patient in need thereof.
15. A method of producing CRF, the method comprising the steps of obtaining a blood sample from a goat; separating the serum from the remaining blood components; and purifying the serum by precipitation of solids.
16. A method for curative, ameliorative, or prophylactic treatment of a disease selected from:
rheumatoid arthritis; optic neuritis; motor neurone disease; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas;
non-demyelinating neural disorders including cerebrovascular ischaemic disease, Alzheimer's disease, Huntingdon's chorea, mixed connective tissue diseases, scleroderma, anaphylaxis, septic shock, carditis and endocarditis, wound healing, contact dermatitis, occupational lung diseases, glomerulnephritis, transplant rejection, temporal arteritis, vasculitic diseases, hepatitis, burns, multiple system atrophy, epilepsy, muscular dystrophy, schizophrenia, bipolar disorder, depression, channelopathies, myaesthenia gravis, pain due to malignant neoplasia, chronic fatigue syndrome, fibromyositis, irritable bowel syndrome, work related upper limb disorder, cluster headache, migraine, and chronic daily headache;
demyelinating disorders including infections of the nervous system, nerve entrapment and focal injury, traumatic spinal cord injury, brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy; channelopathies; and tic douloureux;
autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
inflammatory conditions;
hereditary motor and sensor neuropathy of all types; Charcot-Marie-Tooth disease (CMT) types CMTIA, CMTIB, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy /
Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies (HSAN1, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSANS); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease; Fabry's Disease; Adrenoleukodystrophy;
Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCA10, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia;
Cockayne's syndrome; and Giant axonal neuropathy;
chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome;
canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders;
comprising administering CRF to a patient.
rheumatoid arthritis; optic neuritis; motor neurone disease; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas;
non-demyelinating neural disorders including cerebrovascular ischaemic disease, Alzheimer's disease, Huntingdon's chorea, mixed connective tissue diseases, scleroderma, anaphylaxis, septic shock, carditis and endocarditis, wound healing, contact dermatitis, occupational lung diseases, glomerulnephritis, transplant rejection, temporal arteritis, vasculitic diseases, hepatitis, burns, multiple system atrophy, epilepsy, muscular dystrophy, schizophrenia, bipolar disorder, depression, channelopathies, myaesthenia gravis, pain due to malignant neoplasia, chronic fatigue syndrome, fibromyositis, irritable bowel syndrome, work related upper limb disorder, cluster headache, migraine, and chronic daily headache;
demyelinating disorders including infections of the nervous system, nerve entrapment and focal injury, traumatic spinal cord injury, brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy; channelopathies; and tic douloureux;
autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
inflammatory conditions;
hereditary motor and sensor neuropathy of all types; Charcot-Marie-Tooth disease (CMT) types CMTIA, CMTIB, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy /
Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies (HSAN1, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSANS); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease; Fabry's Disease; Adrenoleukodystrophy;
Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCA10, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia;
Cockayne's syndrome; and Giant axonal neuropathy;
chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome;
canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders;
comprising administering CRF to a patient.
17. A method for curative, ameliorative, or prophylactic treatment of a disease selected from:
rheumatoid arthritis; optic neuritis; motor neurone disease; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas;
non-demyelinating neural disorders including cerebrovascular ischaemic disease, Alzheimer's disease, Huntingdon's chorea, mixed connective tissue diseases, scleroderma, anaphylaxis, septic shock, carditis and endocarditis, wound healing, contact dermatitis, occupational lung diseases, glomerulnephritis, transplant rejection, temporal arteritis, vasculitic diseases, hepatitis, burns, multiple system atrophy, epilepsy, muscular dystrophy, schizophrenia, bipolar disorder, depression, channelopathies, myaesthenia gravis, pain due to malignant neoplasia, chronic fatigue syndrome, fibromyositis, irritable bowel syndrome, work related upper limb disorder, cluster headache, migraine, and chronic daily headache;
demyelinating disorders including infections of the nervous system, nerve entrapment and focal injury, traumatic spinal cord injury, brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy; channelopathies; and tic douloureux;
autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
inflammatory conditions;
hereditary motor and sensor neuropathy of all types; Charcot-Marie-Tooth disease (CMT) types CMT1A, CMT1B, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy /
Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies (HSAN1, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSAN5); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease; Fabry's Disease; Adrenoleukodystrophy;
Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCA10, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia;
Cockayne's syndrome; and Giant axonal neuropathy;
chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome;
canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders;
comprising administering POMC to a patient.
rheumatoid arthritis; optic neuritis; motor neurone disease; axonal or nerve damage;
cancers, in particular myelomas, melanomas, and lymphomas;
non-demyelinating neural disorders including cerebrovascular ischaemic disease, Alzheimer's disease, Huntingdon's chorea, mixed connective tissue diseases, scleroderma, anaphylaxis, septic shock, carditis and endocarditis, wound healing, contact dermatitis, occupational lung diseases, glomerulnephritis, transplant rejection, temporal arteritis, vasculitic diseases, hepatitis, burns, multiple system atrophy, epilepsy, muscular dystrophy, schizophrenia, bipolar disorder, depression, channelopathies, myaesthenia gravis, pain due to malignant neoplasia, chronic fatigue syndrome, fibromyositis, irritable bowel syndrome, work related upper limb disorder, cluster headache, migraine, and chronic daily headache;
demyelinating disorders including infections of the nervous system, nerve entrapment and focal injury, traumatic spinal cord injury, brachial plexopathy (idiopathic and traumatic, brachial neuritis, parsonage turner syndrome, neuralgic amyotrophy); radiculopathy; channelopathies; and tic douloureux;
autoimmune diseases including lupus, psoriasis, eczema, thyroiditis, and polymyositis;
inflammatory conditions;
hereditary motor and sensor neuropathy of all types; Charcot-Marie-Tooth disease (CMT) types CMT1A, CMT1B, CMT2, CMT3 (Dejerine Sottas disease), CMT4 (Types A, B C and D), X-linked Charcot-Marie-Tooth disease (CMTX); Hereditary Neuropathy with liability to pressure palsies (HNPP) - also called Tomaculous neuropathy; Hereditary Motor and Sensory Neuropathy with Deafness - Lom (HMSNL); Proximal Hereditary Motor and Sensory Neuropathy /
Neuronopathy (HMSNP); Hereditary Neuralgic Amyotrophy; Hereditary Sensory and Autonomic Neuropathies (HSAN1, HSAN2, HSAN3 (also called Riley-Day syndrome or familial dysautonomia), HSAN4, HSAN5); Familial Amyloid polyneuropathies (Type I, Type II, Type III, Type IV); Metachromatic Leukodystrophy; Krabbe's Disease; Fabry's Disease; Adrenoleukodystrophy;
Refsum's disease (HMSN IV); Tangier Disease; Friedreich's ataxia; Spinal cerebellar ataxia (SCA) all types - SCA1, SCA2, SCA3, SCA4, SCA5, SCA6, SCA7, SCA8, SCA10, SCA11, SCA12, SCA13, SCA14, SCA16; Spinocerebellar Ataxia;
Cockayne's syndrome; and Giant axonal neuropathy;
chronic inflammatory demyelinating polyneuropathy (CIDP), and Guillain-Barre syndrome;
canine atopic dermatitis, canine oral melanoma, and equine pulmonary disorders;
comprising administering POMC to a patient.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0415359.9A GB0415359D0 (en) | 2004-07-08 | 2004-07-08 | Medicament |
GB0415359.9 | 2004-07-08 | ||
GB0423386.2 | 2004-10-21 | ||
GBGB0423386.2A GB0423386D0 (en) | 2004-07-08 | 2004-10-21 | Medicament |
PCT/GB2005/050108 WO2006021814A2 (en) | 2004-07-08 | 2005-07-08 | Medicament |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2572777A1 true CA2572777A1 (en) | 2006-03-02 |
Family
ID=35967903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002572777A Abandoned CA2572777A1 (en) | 2004-07-08 | 2005-07-08 | Medicament comprising corticotropin releasing factor and proopiomelanocortin, and uses thereof |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1765377A2 (en) |
JP (1) | JP2008505878A (en) |
KR (1) | KR101235723B1 (en) |
AU (1) | AU2005276242B2 (en) |
BR (1) | BRPI0513062A (en) |
CA (1) | CA2572777A1 (en) |
WO (1) | WO2006021814A2 (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0415359D0 (en) * | 2004-07-08 | 2004-08-11 | Aimsco Ltd | Medicament |
GB0509052D0 (en) * | 2005-05-04 | 2005-06-08 | Aimsco Ltd | Combination therapy |
GB0600202D0 (en) * | 2006-01-06 | 2006-02-15 | Aimsco Ltd | Treatment of HIV |
EP2081587A2 (en) | 2006-10-18 | 2009-07-29 | Research Development Foundation | Alpha-msh therapies for treatment of autoimmune disease |
RU2010114006A (en) * | 2007-09-11 | 2011-10-20 | Мондобайотек Лабораториз Аг (Li) | APPLICATION OF A PEPTIDE AS A THERAPEUTIC |
WO2009040083A2 (en) * | 2007-09-11 | 2009-04-02 | Mondobiotech Laboratories Ag | Use of a peptide as a therapeutic agent |
EP2185182A1 (en) * | 2007-09-11 | 2010-05-19 | Mondobiotech Laboratories AG | Use of grf-1 (1-29 ) and corticotropin-releasing factor as therapeutic agents |
JP2011519375A (en) * | 2008-04-30 | 2011-07-07 | ニュートロン ロウ | Use of corticotropin-releasing factor for cancer treatment |
US20100203048A1 (en) | 2008-04-30 | 2010-08-12 | Stephen Evans-Freke | Methods of using corticotropin-releasing factor for the use of the treatment of cancer |
GB0910032D0 (en) * | 2009-06-11 | 2009-07-22 | Aimsco Ltd | Use of serum composition to reduce the levels of TNF and/or VEGF in mammals |
US20130259875A1 (en) * | 2010-05-11 | 2013-10-03 | Questcor Pharmaceuticals, Inc. | Acth for treatment of amyotrophic lateral sclerosis |
EP2701728B1 (en) | 2012-06-25 | 2015-05-06 | Aimsco Limited | Formulation comprising crh and alpha-2-macroglobulin |
WO2015033175A1 (en) * | 2013-09-06 | 2015-03-12 | Zlatko Ademovic | Combination of pro-opiomelanocortin endogenous peptides in autoimmune disease treatment |
GB201322948D0 (en) * | 2013-12-23 | 2014-02-12 | Aimsco Ltd | Improved formulation |
US10006027B2 (en) | 2014-03-19 | 2018-06-26 | Ionis Pharmaceuticals, Inc. | Methods for modulating Ataxin 2 expression |
AU2015231130C1 (en) | 2014-03-19 | 2021-12-23 | Ionis Pharmaceuticals, Inc. | Compositions for modulating Ataxin 2 expression |
GB201617175D0 (en) | 2016-10-10 | 2016-11-23 | Iconic Intellectual Property Limited | Assay |
AR115847A1 (en) | 2018-07-25 | 2021-03-03 | Ionis Pharmaceuticals Inc | COMPOUNDS AND METHODS TO REDUCE THE EXPRESSION OF ATXN2 |
GB201911064D0 (en) | 2019-08-02 | 2019-09-18 | Tarian Biologics Ltd | Therapy |
GB201911063D0 (en) | 2019-08-02 | 2019-09-18 | Tarian Biologics Ltd | Formulations |
KR102551880B1 (en) * | 2020-11-13 | 2023-07-05 | 성균관대학교산학협력단 | Composition for promoting the formation of neurosphere comprising Corticotropin-releasing hormone as an active ingredient |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4801612A (en) * | 1986-07-03 | 1989-01-31 | Regents Of The University Of California | Method of inhibiting inflammatory response |
US5137871A (en) * | 1989-07-28 | 1992-08-11 | Regents Of The University Of California | Treatment to reduce edema for brain and musculature injuries |
US5547940A (en) * | 1992-03-16 | 1996-08-20 | Ludwig Institute For Cancer Research | Method for stimulating proliferation of colon cells using POMC76-103 |
DE4407489A1 (en) * | 1994-03-07 | 1995-09-14 | Bayer Ag | Vaccine for the prevention of respiratory and reproductive diseases of the pig |
US20030186867A1 (en) * | 2000-03-31 | 2003-10-02 | Laura Facci | Use of crf receptor agonists for the treatment or prophylaxis of diseases, for example neurodegenerative diseases |
DE50201566D1 (en) * | 2001-02-24 | 2004-12-23 | Mologen Ag | BETA-ENDORPHIN / CRF - GENE THERAPY FOR LOCAL PAINT CONTROL |
EA013517B1 (en) * | 2001-07-02 | 2010-06-30 | Эймско Лимитед | Use of polyclonal anti-hiv goat serum as a therapeutic agent for the preparation of a medicament |
CA2473754C (en) * | 2002-01-28 | 2012-04-10 | Aimsco Limited | Treatment of ms with goat serum |
-
2005
- 2005-07-08 WO PCT/GB2005/050108 patent/WO2006021814A2/en active Application Filing
- 2005-07-08 AU AU2005276242A patent/AU2005276242B2/en not_active Ceased
- 2005-07-08 BR BRPI0513062-0A patent/BRPI0513062A/en not_active IP Right Cessation
- 2005-07-08 EP EP05758978A patent/EP1765377A2/en not_active Withdrawn
- 2005-07-08 KR KR1020077002992A patent/KR101235723B1/en not_active IP Right Cessation
- 2005-07-08 CA CA002572777A patent/CA2572777A1/en not_active Abandoned
- 2005-07-08 JP JP2007519893A patent/JP2008505878A/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
KR101235723B1 (en) | 2013-02-21 |
WO2006021814A3 (en) | 2007-03-29 |
EP1765377A2 (en) | 2007-03-28 |
AU2005276242B2 (en) | 2011-08-25 |
JP2008505878A (en) | 2008-02-28 |
BRPI0513062A (en) | 2008-04-22 |
AU2005276242A1 (en) | 2006-03-02 |
KR20070042546A (en) | 2007-04-23 |
WO2006021814A2 (en) | 2006-03-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2005276242B2 (en) | Medicament | |
US20130203669A1 (en) | Medicament | |
US20180221450A1 (en) | Formulation Comprising A Stabilized Complex Of Corticotropin Releasing Hormone And Alpha-2 Macroglobulin | |
US20140072530A1 (en) | Treatment of hiv | |
IL129211A (en) | Use of colostrinin in the manufacture of a medicament for the treatment of central nervous system disorders | |
KR0159046B1 (en) | Treatment of autoimmune diseases by aerosol administration of autoantigens | |
WO2015097460A1 (en) | Improved formulation | |
EP0273909A1 (en) | Process for treatment of allergies | |
US20130344102A1 (en) | Formulation | |
JP2583248B2 (en) | Immunotherapy | |
WO2006082451A1 (en) | Bioactive compounds | |
Zink et al. | Cumulation and elimination of horse-anti-dog lymphocyte and normal horse gammaglobulin in dogs | |
CN1367247A (en) | Heavy metal toxinicide, its preparation method and application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
FZDE | Discontinued |
Effective date: 20151222 |