CA2517781A1 - Novel cellulase enzymes and systems for their expression - Google Patents
Novel cellulase enzymes and systems for their expression Download PDFInfo
- Publication number
- CA2517781A1 CA2517781A1 CA002517781A CA2517781A CA2517781A1 CA 2517781 A1 CA2517781 A1 CA 2517781A1 CA 002517781 A CA002517781 A CA 002517781A CA 2517781 A CA2517781 A CA 2517781A CA 2517781 A1 CA2517781 A1 CA 2517781A1
- Authority
- CA
- Canada
- Prior art keywords
- egi
- truncated
- catalytic core
- trichoderma
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010059892 Cellulase Proteins 0.000 title claims abstract 7
- 230000002538 fungal effect Effects 0.000 claims abstract 4
- 108010084185 Cellulases Proteins 0.000 claims abstract 3
- 102000005575 Cellulases Human genes 0.000 claims abstract 3
- 241000223262 Trichoderma longibrachiatum Species 0.000 claims abstract 3
- 230000003197 catalytic effect Effects 0.000 claims 4
- 241000223259 Trichoderma Species 0.000 claims 2
- 101710132601 Capsid protein Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 229940106157 cellulase Drugs 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 108010091371 endoglucanase 1 Proteins 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.
Claims (3)
1. A truncated fungal cellulase protein derived from Trichoderma comprising an endoglucanase I ("EGI") catalytic core protein or derivative thereof which exhibit endoglucanase activity, wherein said protein lacks cellulose binding acitivity from an EGI binding domain, said cellulase being produced by a method comprising the steps of:
(a) transforming into a suitable host cell a DNA construct which encodes said EGI catalytic core which is functionally attached to regulatory sequences which permit the transcription and translation of said DNA;
(b) growing said host cell under conditions suitable to express said EGI
catalytic core.
(a) transforming into a suitable host cell a DNA construct which encodes said EGI catalytic core which is functionally attached to regulatory sequences which permit the transcription and translation of said DNA;
(b) growing said host cell under conditions suitable to express said EGI
catalytic core.
2. The truncated fungal cellulase protein according to claim 1 wherein said Trichoderma is Trichoderma longibrachiatum.
3. The truncated fungal cellulase protein according to claim 1 wherein said EGI
catalytic core consists of the amino acid sequence set forth in SEQ ID NO:14.
catalytic core consists of the amino acid sequence set forth in SEQ ID NO:14.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/169,948 | 1993-12-17 | ||
| US08/169,948 US5861271A (en) | 1993-12-17 | 1993-12-17 | Cellulase enzymes and systems for their expressions |
| CA002178636A CA2178636A1 (en) | 1993-12-17 | 1994-12-19 | Novel cellulase enzymes and systems for their expression |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002178636A Division CA2178636A1 (en) | 1993-12-17 | 1994-12-19 | Novel cellulase enzymes and systems for their expression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2517781A1 true CA2517781A1 (en) | 1995-06-22 |
| CA2517781C CA2517781C (en) | 2010-11-30 |
Family
ID=35589260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2517781A Expired - Lifetime CA2517781C (en) | 1993-12-17 | 1994-12-19 | Novel cellulase enzymes and systems for their expression |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2517781C (en) |
-
1994
- 1994-12-19 CA CA2517781A patent/CA2517781C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2517781C (en) | 2010-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20141219 |