CA2512535A1 - Pipette tip holder for an automated pipette machine - Google Patents

Abstract

A pipette tip holder is provided for holding a plurality of pipette tips in an automated pipette machine. The pipette tip holder includes a container that defines a cavity. There is at least one vertical divider in the cavity that divides the cavity into a plurality of segregated compartments. The pipette tip holder further includes a tip support member. The tip support member is connected to the container. The tip support member defines a plurality of apertures. The apertures are sized to receive and suspend said pipette tips in the cavity.
Each aperture is generally aligned with one of said compartments. The at least one vertical divider is sized and positioned to inhibit contact between bottom portions of adjacent pipette tips.

Description

Title: Pipette Tip Holder For An Automated Pipette Machine Field of the Invention This invention relates generally to pipette tip holders, and more particularly to pipette tip holders or boxes for use with automated pipette machines.
Background of the Invention Automatic pipette machines or robots are used in the chemical and biological fields to automatically pipette fluids from one place to another, without the need for direct human involvement. Such machines typically load disposable pipette tips from a tip box or tip holder onto a pipette head. Conventional tip boxes generally comprise of a tray with individual holes in which the pipette tips are suspended, and are not designed with the concept of replacing a used tip in the rack. Replacing used tips in the rack has the advantages of eliminating a separate waste container for used tips and allowing the tips to be reused whenever the particular analysis method allows for such reuse. However, by placing a used tip next to an unused tip or next to a tip used to pipette a different fluid, the chance for cross contamination between the tips arises either from direct transfer of fluid through contact between the tips or indirect transfer through splashing from drops falling from the used tips. In addition, some chemical or biological processes require significantly different volumes of fluid to be pipetted at various steps of the analysis, in which case an automated pipetting machine would require different sized pipette tips to transfer the different volumes accurately. However, conventional tip boxes accommodate only one volumetric size of pipette tip at a time. If two different pipette tip sizes are needed then two different types of tip box are necessary. This takes up valuable space on the limited working area of the pipetting machine.
It is desirable to provide a pipette tip holder that reduces that chance of cross contamination of pipette tips so as to enable the reuse of pipette tips in chemical or biological analysis processes. It is also desirable to provide such pipette tip holder that can also accommodate more than one volumetric size of pipette tip, and be relatively easy and inexpensive to fabricate.

Summary of the Invention In a first aspect, the invention is directed to a pipette tip holder for use with automated pipette machines comprising a first container having a bottom and sides to define a first cavity and a top edge portion defining an opening to said first cavity, a plurality of vertical dividers within the first cavity connected to the bottom and the sides of the first container for dividing the first cavity into segregated compartments or reservoirs for limiting or preventing the flow of fluid between said compartments or reservoirs when the first container is upright, and a tip support member connected to the first container and being adapted to suspend a plurality of pipette tips within the first cavity such that at least a bottom portion of each pipette tip is within an individual reservoir, thereby segregating the pipette tips to reduce the possibility of fluid from one tip contacting another tip in the pipette tip holder. In some embodiments, the tip support member is adapted to suspend a plurality of pipette tips of more than one size within the first cavity.
In some embodiments, the tip support member comprises a lid member over the opening of the first container, the lid member defining a plurality of apertures for receiving the pipette tips, each aperture being aligned with a reservoir and being adapted to suspend a pipette tip within said reservoir. The lid member may define apertures of more than one size, each aperture being adapted to suspend a pipette tip of a certain size within a reservoir. The lid member may be removable from the first container. The lid member, in some embodiments, comprises a planar top in which the apertures are defined connected to sides to form an inverted second container defining a second cavity, and having a bottom edge portion on said sides defining an opening to the second cavity, the bottom edge portion being adapted to releasably connect to the top edge portion of the first container such that the second cavity merges with the first cavity when the lid member is connected to the first container.
The lid member in some embodiments includes a plurality of vertical dividers within the second cavity connected to the planar top and the sides of the second container for dividing the second cavity into segregated compartments that align with the reservoirs of the first container when the lid member is connected to the first container.
In some embodiments, the first container includes a first connecting portion on the top edge portion, and the lid member includes a second connecting portion on the bottom edge portion that is adapted to closely fit into the first connecting portion.
The first connecting portion may further include an inward facing groove, and the second connecting portion may further include an outward facing ridge that is complimentary to the groove such that the ridge closely fits into the groove to provide a snap fit engagement between the lid member and the first container. A cap adapted for a close releasable connection over the lid member to provide a cover for pipette tips being supported by the lid member may be provided in some embodiments.
In another aspect, the invention is directed to a pipette tip holder for holding a plurality of pipette tips in an automated pipette machine. The pipette tip holder includes a container that defines a cavity, at least one vertical divider in the cavity for dividing the cavity into a plurality of segregated compartments, and a tip support member. The tip support member is connected to the container. The tip support member defines a plurality of apertures. The apertures are sized to receive and suspend said pipette tips in the cavity. Each aperture is generally aligned with one of said compartments. The at least one vertical divider is sized and positioned to inhibit contact between bottom portions of adjacent pipette tips.
In one embodiment of this aspect, the cavity in the container is a first cavity and the tip support member defines a second cavity. The tip support member is connectable with the container such that the first and second cavities communicate with each other and together form an internal cavity for receiving the pipette tips.
In a further embodiment of this aspect, the at least one vertical divider is sealingly connected to the container such that each compartment is sized to hold a volume of fluid and thereby inhibit a flow of fluid contained therein to any other of said compartments.
Brief Description of the Drawings For a better understanding of the present invention and to show more clearly how it may be carried into effect, reference will now be made by way of example to the accompanying drawings, in which:
Fig. 1 is an exploded view of a pipette tip holder in accordance with an embodiment of the present invention;

Fig. 2 is a perspective view of the pipette tip holder of Fig.1 loaded onto a carousel of an automatic pipetting machine;
Fig. 3 is a front elevation view of the container of the pipette tip holder of Fig.
1;
Fig. 4 is a side elevation view of the container showing the dividers within the cavity using broken lines;
Fig. 5 is a section view of the container through line 5-5 shown in Fig. 3;
Fig 6 is a detailed view of area B of the container as shown in Fig. 5;
Fig. 7 is a top plan view of the container of Fig. 1;
Fig. 8 is a front elevation view of the lid member of Fig. 1;
Fig. 9 is a side elevation view of the lid member of Fig. 1;
Fig. 10 is a section view of the lid member through line 10-10 shown in Fig.
8;
Fig. 11 is a detailed view of area D of the lid member as shown in Fig. 10;
Fig. 12 is a section view of the lid member through line 12-12 of Fig. 8;
Fig 13 is a front elevation view of the cap of Fig. 1;
Fig 14 is a section view of the cap through line 14-14 as shown in Fig. 13;
Fig. 15 is a bottom plan view of the cap of Fig. 1;
Fig 16 is a section view of another container for use in a pipette tip holder in accordance with another embodiment of the present invention; and Fig 17 is a section view of yet another container for use in a pipette tip holder in accordance with another embodiment of the present invention.
Detailed Description of the Drawings In the accompanying drawings, like numerals indicate the same elements. It will be understood that the present disclosure is an exemplification of the principles of the invention and does not limit the invention to the illustrated embodiments.
Therefore, specific details disclosed herein are not to be interpreted as limiting, but rather as a basis for the claims and as a representative basis for teaching one skilled in the art to employ the present invention.
Referring to Figure 1, there is illustrated in an exploded view a pipette tip holder or pipette tip box 10 in accordance with an embodiment of the present invention. Pipette tip box 10 comprises a container 12 and a tip support member such as lid member 14, and optionally also includes a cap 16. Container 12, lid member 14 and cap 16 may be similarly dimensioned in cross-section to that of an isosceles trapezoid with rounded corners. Lid member 14 is adapted for a snap-fit engagement with container 12, and cap 16 is designed to fit over and engage a top portion of lid member 14, as described below. In Figure 2, pipette tip box 10 without the cap 16 is shown holding a plurality of tips 7, including a tip 6 of a first size, and two tips 8 of a second size, which is different from the first size. The tip holder 10 is positioned within a complementarily dimensioned aperture 4 of a carousel 5 of an automated pipette machine (not shown). It will be apparent to persons skilled in the art that the tip box 10 may be otherwise shaped or dimensioned for use with particular sizes of pipette tips and/or with particular automated pipette machines.
While the tip holder 10 is shown holding two different sizes of tips 7, it is possible for the tip folder 10 to be configured to hold more than two different sizes of tip 7.
Preferably, the container 12, lid member 14 and cap 16 are made of a suitable injection molded plastic for ease of production and low cost.
Referring to Figures 3-7, container 12 comprises a bottom 18 (Figure 3) connected to sides or side walls 20, 21, 22, and 23, (Figure 7) to define an internal cavity 24 (Figure 5) and a top edge portion 26 that defines the opening of container 12. Of course, in alternative embodiments of the present invention, container may be cylindrical, in which case the side member would comprise of a cylindrical tube, or if the container were a square, then the side member would comprise four equal side walls. Portions of the internal surfaces of side walls 20, 21, 22, and 23 proximate the top edge portion 26 define a channel 28 (Figure 6) that extends around the perimeter of container 12 thereby providing a shoulder 29. Channel thereby provides a first connecting portion of container 12. The portion of side walls 20, 21, 22, and 23 located within channel 28 further defines a semicircular groove 30 that faces inward and extends around at least a part of the perimeter of container 12.
On top edge 26 of side wall 23 are defined two equally spaced notches 25 which are discussed further below.
Within a lower two-thirds of container 12 is provided a central vertical divider 32 that extends between side walls 21 and 22 and joins with bottom 18, to divide the lower portion of cavity 24 (Figure 4) into two sections which may be of unequal size.
These two sections are further divided by vertical dividers 33, 34, and 35 (Figure 7) that extend between central divider 32 and side walls 23 and 20 respectively, and join with bottom 18. The dividers 32, 33, 34 and 35 divide the lower portion of cavity 24 into separate compartments 36, 37, 38, 39 and 40. The dividers separate the bottom portions of a plurality of pipette tips 7 when the pipette tips 7 are positioned in the tip holder 10. The bottom portions of the pipette tips 7 are those portions of the pipette tips 7 that are immersed in a liquid during use of the pipette tips during use.
In other words, the bottom portion of the pipette tips 7 is the portion whose exterior surface is wetted during use of the tip 7.
The dividers 32, 33, 34 and 35 may be sealingly connected to the container 12, such that the compartments are sealed along their sides and bottom thereby forming reservoirs 36, 37, 38, 39 and 40. The compartments are sized to hold a volume of fluid and thereby inhibit a flow of fluid contained therein to any other of the compartments.
As described in more detail below, the dividers segregate at least the lower portion of the pipette tips from adjacent pipette tips. By sealing the dividers with the bottom and sides of the cavity 24, the reservoirs contain drips from a used pipette tip being held therein to prevent fluid from the used tip from flowing into other reservoirs and under other pipette tips within the tip holder 10.
Referring to Figures 8-12, lid member 14 comprises a planar top 50 connected to sides or side walls 46, 47, 48, and 49, (Figure 12) to form an inverted second container that defines a second or lid cavity 51 and has a bottom edge portion 56 (Figure 8) that defines an opening in the lid member 14. The external surfaces of side walls 46 and 49 (Figures 8 and 9) include gripping portions 54a and 54b respectively. Each gripping portion 54a and 54b comprises a plurality of closely spaced, parallel grooves. A second connecting portion 58 (Figure 9) is provided on the external surface of the side walls 46, 47, 48, and 49 (Figure 12) proximate the bottom edge 56 (Figure 9) of lid member 14. Second connecting portion 58 is dimensioned to closely fit into channel 28 (Figure 6) of container 12. Within second connecting portion 58 (Figure 11) on at least a part of each side 46, 47, 48, and 49 (Figure 12) is provided a semi-circular ridge 60 (Figure 11 ) that faces outward and is adapted to closely fit into, and thereby engage, groove 30 (Figure 6) of container 12 to provide a snap-fit between the lid member 14 (Figure 10) and the container (Figure 5). A portion of lid member 14 (Figure 8) above and adjacent to second connecting portion 58 is provided with a peripheral lip 64 that is adapted to seat against top edge 26 (Figure 5) of container 12. On bottom edge 56 of side wall (Figure 9) are provided two equally spaced projections 66 (Figure 12) that spatially correspond with, and are adapted to fit into, notches 25 (Figure 7) on the container 12. The projections 66 are used to position the tip box 10 within the aperture (Figure 2) of the carousel.
Within the second cavity of lid member 14 (Figure 12) is provided a central divider 70 that extends between side walls 47 and 48, and joins with top member 50 to divide the cavity into two. These are further divided by sub-dividers 71 and 72, 73 that extend between central divider 70 and side wall 46 and 49 respectively, and join with top member 50. Accordingly, the cavity of lid member 14 is divided into separate compartments 75, 76, 77, 78 and 79 thereby preventing contact between adjacent tips as a tip is being inserted into the tip holder by the pipette machine.
Referring to Figure 12, top member 50 defines apertures such as holes 80, 81, 82, 83 and 84, each of which is aligned within the boundaries of a compartment.
The holes are sized to accommodate a desired size or shape of pipette tip and to suspend the main portion of the tip within the tip box 10. With lid member 14 (Figure 12) connected to container 12 (Figure 7), the cavities 51 and 24 communicate with each other and form an internal cavity. Additionally, central divider 70 (Figure 12) aligns with central divider 32, (Figure 7) and dividers 71, and 72 and 73 (Figure 12) align with dividers 35, and 34 and 33 (Figure 7) respectively. Accordingly, compartments 75, 76, 77, 78 and 79 (Figure 12) in lid member 14 align with reservoirs 36, 37, 38, 39 and 40 (Figure 7) respectively in container 12.
Thus, each pipette tip suspended from the top member 50 is within the boundaries of its own compartment-reservoir combination and is thereby shielded from contact with the fluid of adjacent pipette tips. In the illustrated embodiment, holes 80 and 81 and corresponding compartment-reservoir combinations 75-36 and 76-37 are sized to accommodate a larger pipette tip, such as a 5mL tip; whereas, holes 82, 83 and and corresponding compartment-reservoir combinations 77-38, 78-39 and 79-40 are sized to accommodate a smaller pipette tip, such as a 1mL tip. Preferably, the size and the number of pipette tips that the tip holder 10 can accommodate matches the size and the number of tips required to process a given sample. For example, the illustrated embodiment is designed to hold the size and number of tips required for a specific magnetic cell separation process. However, the present invention can be _g_ applied to other automated pipetting processes.
By manufacturing the lid member 14 so that it defines a cavity, the manufacture of the container 12 is simplified, since it can be made without having unduly deep, narrow aspect-ratio reservoirs which can represent a challenge to manufacture using certain processes, such as some injection molding processes.
By providing dividers 70, 71, 72 and 73 (Figure 12) on the lid member 14, which mate with the dividers 32, 33, 34 and 35 (Figure 7) on the container 12, deep individual compartments that are each defined in part by dividers of the container and in part by dividers of the tip support member, whereby adjacent pipette tips 7 are separated substantially entirely from each other within the tip support member and container 12.
It is alternatively possible for the lid member 14 to not include a second container (ie. the second cavity). For example, the lid member 14 could be a plate similar to top member 50 and could contain apertures 80, 81, 82, 83 and 84 for permitting the pass-through of pipettes. In this alternative, the lid member 14 could, for example, be provided with attachment tabs that connect the lid member 14 to the container 12 (Figure 1). This alternative embodiment retains the advantage that the dividers permit the separation of at least the bottom portions of adjacent pipette tips, while using less material and/or with a reduced manufacturing difficulty than some tip holders of the prior art.
It is alternatively possible for the lid member 14 to have a second cavity, but to not include dividers therein. Providing the second cavity, even if it does not contain dividers, still provides the advantage that the container 12 can be made with compartments with a reduction in difficulties associated with molding of high aspect ratio compartments.
By making the lid member 14 removable from the container 12 (Figure 1 ), cleanout of the tip holder 10 is facilitated after it has been used. It is alternatively possible, however, for the lid member 14 to be non-removably mounted to the container 12. For example, the lid member 14 could be an injection molded piece that is attached to the container 12 by an adhesive.
Referring to Figures 13-15, cap 16 comprises sides 90, 91, 92 and 93 connected to top 94 to define a cavity within the cap 16. Cap 16 is dimensioned to closely fit over lid member 14 (Figure 1) so that the tops of pipettes supported in the lid member are enclosed by the cap 16. This facilitates transport of the tip holder 10 with tips therein, while maintaining sterility of the tips. Within the cavity of cap 16 on each of sides 90, 91 and 92 are provided flanges 96 (Figure 15) that originates at top 94 and extends approximately three-fourths of the distance down sides 90, 91 and 92, each flange terminating in a straight edge. When the cap is placed over the lid member 14 (Figure 9), the flanges 96 (Figure 15) abut top member 50 (Figure 9) of the support member 14 thereby providing a stop for the cap. Ridges 97 on the inside surface of sides 90 and 93 engage the complimentary grooves on the gripping portions 54a and 54b (Figure 9) for holding the cap 16 (Figure 14) on the lid member 14 (Figure 9).
Figure 2 shows the illustrated embodiment of the pipette tip box in use in a carousel of a 8-z-A automated pipette machine which combines rotational (8) and vertical (z) motion of a robot arm holding the tip head with rotational (6) motion of the carousel that holds the pipette tip boxes and the samples, thereby allowing the tip head to access the tips and the samples on the carousel. The pipette machine picks up the desired pipette tip from the box and returns it to the tip box after completing the pipetting operation. The pipette machine can then pick up a tip of a different size as desired from the tip box 10. After each operation, the tips may be returned to the tip box 10 whereby the compartmentalization of the tips reduces the chance of contamination of the tips by contact with adjacent tips or from fluid from other tips within the tip box. It will be apparent to persons skilled in the art that the tip box 10 may also be used in the more common class of robots known as x-y-z gantry style robots where the moveable tip head moves along one vertical axis and two orthogonal horizontal axes of motion.
Referring to Figure 16, a container in accordance with another embodiment of the present invention is generally indicated at 112. Container 112 is similar to container 12 (Figures 3-7), except that the vertical dividers extend from the side walls and do not join with the bottom wall of the container, as is exemplified by central vertical divider 114. Accordingly, the vertical dividers of container 112 define segregated compartments 116 within the container for reducing the possibility of contamination between adjacent pipette tips as the tips are inserted and stored within the pipette tip box 10 between uses, rather than having individual reservoirs as in container 12 (Figures 3-7) with dividers that seal at the bottom and sides to - 1~ -prevent the flow of fluid between the reservoirs.
Referring to Figure 17, a container in accordance with yet another embodiment of the present invention is shown generally at 212. Container 212 may be similar to container 12 (Figures 3-7) except that the vertical dividers, one of which is shown at 215, comprise a plurality of divider segments as is exemplified by segments 216 and 218. The lowest of the divider segments (eg. segment 216) may be sealed at the bottom and sides of the cavity thereby forming a reservoir that prevents fluid flow between adjacent reservoirs.
It has been shown and described for the container 12 to have four dividers in its cavity 24, dividing the cavity 24 into five compartments. It is alternatively possible for the container 12 to have fewer or more dividers thereby dividing the cavity 24 into fewer or more compartments. For example, the container could have as few as one divider, thereby dividing the container 12 into two compartments.
Other configurations of the vertical dividers are possible, such as for example, dividers that extend upwards from the bottom wall of the container but do not join with the sides (not illustrated), provided that some physical barrier exists between adjacent pipette tips to reduce the possibility of contamination as the tips are inserted and stored within the pipette tip box between uses.
While the above description constitutes the preferred embodiments, it will be appreciated that the present invention is susceptible to modification and change without departing from the fair meaning of the accompanying claims.

Claims (27)

1. A pipette tip holder for use with automated pipette machines comprising:
a first container having a bottom and sides to define a first cavity and a top edge portion defining an opening to said first cavity;
a plurality of vertical dividers within the first cavity for dividing the first cavity into segregated compartments; and a tip support member connected to the first container and being adapted to suspend a plurality of pipette tips within the first cavity in a manner so that at least a bottom portion of each pipette tip is within an individual segregated compartment.

2. A pipette tip holder for use with automated pipette machines comprising:
a first container having a bottom and sides to define a first cavity and a top edge portion defining an opening to said first cavity;
a plurality of vertical dividers within the first cavity connected to the bottom and the sides of the first container for dividing the first cavity into segregated reservoirs for preventing the flow of fluid between said reservoirs when the first container is upright; and a tip support member connected to the first container and being adapted to suspend a plurality of pipette tips within the first cavity in a manner so that at least a bottom portion of each pipette tip is within an individual reservoir, thereby segregating the pipette tips to reduce the possibility of fluid from one tip contacting another tip in the pipette tip holder.

3. The apparatus as claimed in any one of claims 1 and 2, wherein the tip support member comprises a lid member over the opening of the first container, the lid member defining a plurality of apertures for receiving the pipette tips, each aperture being approximately aligned with a reservoir or segregated compartment, and being adapted to suspend a pipette tip within said reservoir or segregated compartment.

4. The apparatus as in claim 3 wherein the lid member is removable from the first container.

5. The apparatus as in claim 4 wherein the lid member comprises a planar top in which the apertures are defined connected to sides to form an inverted second container defining a second cavity, and having a bottom edge portion defining an opening to the second cavity, the bottom edge portion being adapted for a close releasable connection to the top edge portion of the first container in a manner so that the second cavity merges with the first cavity when the lid member is connected to the first container.

6. The apparatus as in claim 5 wherein the lid member includes a plurality of vertical dividers within the second cavity connected to the planar top and the sides of the second container for dividing the second cavity into segregated compartments that approximately align with the reservoirs or segregated compartments of the first container when the lid member is connected to the first container.

7. The apparatus as in claim 6 wherein the first container includes a first connecting portion on the top edge portion, and the lid member includes a second connecting portion on the bottom edge portion that is adapted to closely fit into the first connecting portion.

8. The apparatus as in claim 7 wherein the first connecting portion further includes an inward facing groove, and the second connecting portion further includes an outward facing ridge that is complimentary to the groove such that the ridge closely fits into the groove to provide a snap fit engagement between the lid member and the first container.

9. The apparatus as in claim 8 further comprising a cap adapted for a close releasable connection over the lid member to provide a cover for pipette tips being supported by the lid member.

10. A pipette tip holder for use with automated pipette machines comprising:
a first container having a bottom and sides to define a first cavity, and a top edge portion defining an opening to said first cavity;
a plurality of vertical dividers within the first cavity for dividing the first cavity into segregated compartments; and a tip support member connected to the first container and being adapted to suspend a plurality of pipette tips of more than one size within the first cavity in a manner so that at least a bottom portion of each pipette tip is within an individual segregated compartment.

11. A pipette tip holder for use with automated pipette machines comprising:
a first container having a bottom and sides to define a first cavity, and a top edge portion defining an opening to said first cavity;
a plurality of vertical dividers within the first cavity connected to the bottom and the sides of the first container for dividing the first cavity into segregated reservoirs for preventing the flow of fluid between said reservoirs when the first container is upright; and a tip support member connected to the first container and being adapted to suspend a plurality of pipette tips of more than one size within the first cavity in a manner so that at least a bottom portion of each pipette tip is within an individual reservoir, thereby segregating the pipette tips to reduce the possibility of fluid from one tip contacting another tip in the pipette tip holder.

12. The apparatus as claimed in any one of claims 10 and 11, wherein the tip support member comprises a lid member over the opening of the first container, the lid member defining a plurality of apertures of more than one size for receiving the pipette tips, each aperture being approximately aligned with a reservoir or segregated compartment, and being adapted to suspend a pipette tip of a certain size within said reservoir or segregated compartment.

13. The apparatus as in claim 12 wherein the lid member is removable from the first container.

14. The apparatus as in claim 13 wherein the lid member comprises a planar top in which the apertures are defined connected to sides to form an inverted second container defining a second cavity, and having a bottom edge portion defining an opening to the second cavity, the bottom edge portion being adapted for a close releasable connection to the top edge portion of the first container in a manner so that the second cavity merges with the first cavity when the lid member is connected to the first container.

15. The apparatus as in claim 14 wherein the lid member includes a plurality of vertical dividers within the second cavity connected to the planar top and the sides of the second container for dividing the second cavity into segregated compartments that approximately align with the reservoirs or segregated compartments of the first container when the lid member is connected to the first container.

16. The apparatus as in claim 15 wherein the first container includes a first connecting portion on the top edge portion, and the lid member includes a second connecting portion on the bottom edge portion that is adapted to closely fit into the first connecting portion.

17. The apparatus as in claim 16 wherein the first connecting portion further includes an inward facing groove, and the second connecting portion further includes an outward facing ridge that is complimentary to the groove such that the ridge closely fits into the groove to provide a snap fit engagement between the lid member and the first container.

18. The apparatus as in claim 17 further comprising a cap adapted for a close releasable connection over the lid member to provide a cover for pipette tips being supported by the lid member.

19. A pipette tip holder for holding a plurality of pipette tips in an automated pipette machine, comprising:

a container that defines a cavity;
at least one vertical divider in the cavity, wherein said at least one vertical divider divides the cavity into a plurality of segregated compartments;
and a tip support member connected to the container, wherein the tip support member defines a plurality of apertures, wherein said apertures are sized to receive and suspend said pipette tips in the cavity, wherein each aperture is generally aligned with one of said compartments, wherein the at least one vertical divider is sized and positioned to inhibit contact between bottom portions of adjacent pipette tips.

20. The pipette tip holder as claimed in claim 19, wherein the cavity in the container is a first cavity and wherein the tip support member defines a second cavity, wherein the tip support member is connectable with the container such that the first and second cavities communicate with each other and together form an internal cavity for receiving the pipette tips.

21. A pipette tip holder as claimed in claim 20, wherein the at least one vertical divider is sealingly connected to the container, wherein each compartment is sized to hold a volume of fluid and thereby inhibit a flow of fluid contained therein to any other of said compartments.

22. The pipette tip holder as claimed in claim 21, wherein the plurality of compartments in the container are a first plurality of compartments and wherein the tip support member includes at least one vertical divider in the second cavity for dividing the second cavity into a second plurality of segregated compartments that are aligned with the first compartments in the container when the tip support member is connected to the container.

23. The pipette tip holder as claimed in claim 22, wherein the at least one vertical divider in the second cavity is sealingly connected to the tip support member so that contact between pipette tips in adjacent compartments is substantially entirely prevented within the first and second cavities.

24. The pipette tip holder as claimed in any of claims 19 and 20, wherein the plurality of apertures includes at least a first aperture and a second aperture, wherein the second aperture is different from said first aperture in at least one of size and shape.

25. The pipette tip holder as claimed in claim 20, wherein the container has a top edge portion and wherein the top edge portion defines an opening into the first cavity and wherein the container has a first connecting portion proximate the top edge portion, and wherein the tip support member has a bottom edge portion and wherein the bottom edge portion defines an opening into the second cavity and wherein the tip support member has a second connecting portion proximate the bottom edge portion, wherein the second connecting portion is sized to closely fit removably into the first connecting portion.

26. The pipette tip holder as claimed in claim 25, wherein the first connecting portion further includes an inward facing groove, and the second connecting portion further includes an outward facing ridge that is complementary to the groove such that the ridge closely fits into the groove to provide a snap fit engagement between the tip support member and the container.

27. The pipette tip holder as claimed in any of claims 19 and 20, further comprising a cap, wherein the cap is connectable over the tip support member to cover any pipette tips supported by the tip support member.