CA2489105A1 - Development of a method for employing polystyrene/dextran nabo-particles in immunoassay - Google Patents
Development of a method for employing polystyrene/dextran nabo-particles in immunoassay Download PDFInfo
- Publication number
- CA2489105A1 CA2489105A1 CA 2489105 CA2489105A CA2489105A1 CA 2489105 A1 CA2489105 A1 CA 2489105A1 CA 2489105 CA2489105 CA 2489105 CA 2489105 A CA2489105 A CA 2489105A CA 2489105 A1 CA2489105 A1 CA 2489105A1
- Authority
- CA
- Canada
- Prior art keywords
- particles
- polystyrene
- coated
- nano
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
Abstract
A method, for employing Polystyrene/Dextran nanometer particles sized in 5-300nm in immunoassay, is established. Polystyrene/Dextran nano-particles are coated with protein or protein conjugates which are labeled with fluorescence, enzyme, biotin, magnetic moterial, luminescence material or isotope. The nano-particles coated protein or protein conjugates are used immunochromatography, immunofiltration and other immunoassay. The nano-particles of different sized, coated protein often used analyte, which are arranged like arrays, coated protein conjugates used detector which are machine-readable.
Description
DESCRIPTION:
In immunochromatography assay, Dextran manometer particles is combined with polystreptavidin, and they are formed complex. This complex, as capture reagent, is binding on membrane. The Biotiny labeled antibody is combined capture reagent, and the test line or test spot is formed. FITC
Fluorescein labeled antibody coated polystyrene namo-particle, or antibody coated colored polystyrene namo-particle or colored latex bead, is as detective reagent.
In immunochromatography assay, when sample go through lateral flow system, if there are expected antigen in the sample, Dextran namo-particles polystreptavidin or biotinylated antibody, and antigen or colored latex bead are formed complex which are machine-readable or visual readable. No antigen in the sample, no complex is formed, and the result is negative.
In immunochromatography assay capture reagent also can be treat antibody coated namo-particle bind on membrane. Detective reagent may labeled with fluorescein enzyme, biotin, isotope, magnetic material and/or luminesient material. The protein coated namo-particles may be antibody, antigen, acceptor or ligend. Polystyrene/Dextran namo-particle sized range are 5-300nxn.
In immunochromatography assay, the capture reagent binding on membrane may be single spotlline or mutil-sports which are arranged matrix sports. In immunofiltration assay, polystyrene/Dextran namo-particles coated protein(antibody, antigen, avidin, biotin etc) bind on membrane as capture reagent. The antigen or antibody labeled fluorescein, enzyme, magnetic material or luminesient material may be coated namo- particle as detective reagent. These namo-particles sized ranged are 5-300 mm.
In immunoassay, Dextran namo-particles sized range 5-300 mm, coated with protein such as antigen, antibody on streptavidin, combined with protein namo-particle, may be attached on polystyrene tube or well, as solid support. The namo-particles in the sized reagent coated protein which labeled with flurescein enzyme, magnetic material or luminesient material can be formed a new solid immune test model.
In immunochromatography assay, Dextran manometer particles is combined with polystreptavidin, and they are formed complex. This complex, as capture reagent, is binding on membrane. The Biotiny labeled antibody is combined capture reagent, and the test line or test spot is formed. FITC
Fluorescein labeled antibody coated polystyrene namo-particle, or antibody coated colored polystyrene namo-particle or colored latex bead, is as detective reagent.
In immunochromatography assay, when sample go through lateral flow system, if there are expected antigen in the sample, Dextran namo-particles polystreptavidin or biotinylated antibody, and antigen or colored latex bead are formed complex which are machine-readable or visual readable. No antigen in the sample, no complex is formed, and the result is negative.
In immunochromatography assay capture reagent also can be treat antibody coated namo-particle bind on membrane. Detective reagent may labeled with fluorescein enzyme, biotin, isotope, magnetic material and/or luminesient material. The protein coated namo-particles may be antibody, antigen, acceptor or ligend. Polystyrene/Dextran namo-particle sized range are 5-300nxn.
In immunochromatography assay, the capture reagent binding on membrane may be single spotlline or mutil-sports which are arranged matrix sports. In immunofiltration assay, polystyrene/Dextran namo-particles coated protein(antibody, antigen, avidin, biotin etc) bind on membrane as capture reagent. The antigen or antibody labeled fluorescein, enzyme, magnetic material or luminesient material may be coated namo- particle as detective reagent. These namo-particles sized ranged are 5-300 mm.
In immunoassay, Dextran namo-particles sized range 5-300 mm, coated with protein such as antigen, antibody on streptavidin, combined with protein namo-particle, may be attached on polystyrene tube or well, as solid support. The namo-particles in the sized reagent coated protein which labeled with flurescein enzyme, magnetic material or luminesient material can be formed a new solid immune test model.
Claims (33)
1. A method for immunoassay, which employs nano-meter sized polystyrene/Dextran (polystyrene/dextran nano-particle), wherein said polystyrene nano-particles are coated with proteins or protein conjugation.
2. The method of claim 1, wherein said polystyrene/Dextran nano-particles are colored or not colored.
3. The method of claim 1, wherein said polystyrene/Dextran nano-particles are divided into at least one particle group, particles in different groups being coated with different proteins, for detecting at least one analyte.
4. The method of claim 1, wherein said polystyrene/Dextran nano-particles have at least one size, particles of different sizes being coated with different proteins, for detecting at least one analyte.
5. The method of combination of claim 3 and 4.
6. The method of claim 1, 2, 3, 4, 5, wherein said polystyrene / Dextran nano-particles are in size range of 5nm to 300nm.
7. The method of claim 1, 2, 3, 4, 5, and 6, wherein said coated proteins are antigen, antibody, acceptor, or ligand.
8. An immunoassay, which employs polystyrene/Dextran nano-particle, wherein said polystyrene/Dextran nano-particles are coated with proteins.
9. The immunoassay of claim 8, wherein said polystyrene/Dextran nano-particles are divided into at least one particle group, particles in different groups being coated with different proteins, for detecting at least one analyte.
10. The immunoassay of claim 8, wherein said polystyrene/Dextran nano-particles have at least one size, particles of different sizes being coated with different proteins, for detecting at least one analyte.
11. The immunoassay of combination of claim 9 and 10.
12. The immunoassay of claim 9, 10 and 11, wherein said polystyrene/Dextran nano-particles are colored with at least one color.
13. The immunoassay of claim 8, 9, 11, and 12, wherein said polystyrene/Dextran nano-particles are in size range of 5nm to 300nm.
14. An immunochromatography assay, which employs polystyrene/Dextran nano-particle, wherein said polystyrene/Dextran nano-particles are coated with proteins.
15. The immunochromatography assay of claim 14, wherein said polystyrene/Dextran nano-particles have at least one size, being divided into at least one particle group, being in size range of 5nm to 300nm; said coated proteins are antigen, antibody, acceptor, or ligand.
16. The immunochromatography assay of claim 15 wherein said assay includes three areas in sequence:
a) a sample receiving area, for receiving a liquid sample to be tested;
b) a conjugate area having therein or thereon said polystyrene/Dextran nano-particles in dry state;
c) a read-out area having at least one defined zone, wherein on each said defined zone a complementary binding partner protein to a protein on said particle is immobilized.
The immunochromatography assay works by adding the liquid sample to said sample receiving area, when said sample passing said conjugate area polystyrene/Dextran nano-particles of one or more sizes being released and moving forward together with said sample, said sample carrying said released polystyrene/Dextran nano-particles is then passing said read-out area, said released polystyrene/Dextran nano-particles are thus detected when said sample passes said defined zone(s) in said read-out area, and the analyte in the sample are therefore detected qualitatively and/or quantitatively.
a) a sample receiving area, for receiving a liquid sample to be tested;
b) a conjugate area having therein or thereon said polystyrene/Dextran nano-particles in dry state;
c) a read-out area having at least one defined zone, wherein on each said defined zone a complementary binding partner protein to a protein on said particle is immobilized.
The immunochromatography assay works by adding the liquid sample to said sample receiving area, when said sample passing said conjugate area polystyrene/Dextran nano-particles of one or more sizes being released and moving forward together with said sample, said sample carrying said released polystyrene/Dextran nano-particles is then passing said read-out area, said released polystyrene/Dextran nano-particles are thus detected when said sample passes said defined zone(s) in said read-out area, and the analyte in the sample are therefore detected qualitatively and/or quantitatively.
17. The immunochromatography assay of claim 16, wherein said defined zone(s) at said read-out area have line shape.
18. The immunochromatography assay of claim 17, wherein said line(s) are arranged in the following order:
The complementary binding partner protein(s) immobilized to said line(s) closer to said conjugate area correspond to said protein(s) coated on larger size particles and the closer said line to said conjugate area the larger said corresponding particle size; on the contrary, the complementary binding partner protein(s) immobilized to said line(s) farther to said conjugate area corresponds to said protein(s) coated on smaller size particles and the farther said line to said conjugate area the smaller said corresponding particle size.
The complementary binding partner protein(s) immobilized to said line(s) closer to said conjugate area correspond to said protein(s) coated on larger size particles and the closer said line to said conjugate area the larger said corresponding particle size; on the contrary, the complementary binding partner protein(s) immobilized to said line(s) farther to said conjugate area corresponds to said protein(s) coated on smaller size particles and the farther said line to said conjugate area the smaller said corresponding particle size.
19. The immunochromatography assay of claim 16 wherein said defined zone(s) at said read-out area have spot shape.
20. The immunochromatography assay of claim 16 wherein said defined zones at said read-out area have spot shapes, forming a spot matrix.
21. The immunochromatography assay of claim 19 and 20, wherein said spot(s) are arranged in the following order:
The complementary binding partner protein(s) immobilized to said spot(s) closer to said conjugate area correspond to said protein(s) coated on larger size particles and the closer said spot to said conjugate area the larger said corresponding particle size; on the contrary, the complementary binding partner protein(s) immobilized to said spot(s) farther to said conjugate area corresponds to the protein(s) coated on smaller size particles and the farther said spot to said conjugate area the smaller said corresponding particle size.
The complementary binding partner protein(s) immobilized to said spot(s) closer to said conjugate area correspond to said protein(s) coated on larger size particles and the closer said spot to said conjugate area the larger said corresponding particle size; on the contrary, the complementary binding partner protein(s) immobilized to said spot(s) farther to said conjugate area corresponds to the protein(s) coated on smaller size particles and the farther said spot to said conjugate area the smaller said corresponding particle size.
22. An immunofiltration assay, which employs polystyrene/Dextran nano-particles, wherein said polystyrene/Dextran nano-particles are coated with proteins.
23. The immunofiltration assay of claim 22, wherein said polystyrene/Dextran nano-particles have at least one size, being divided into at least one particle group, and being colored with at least one color, being in size range of 5nm to 300nm; said coated proteins are antigen, antibody, acceptor, or ligand.
24. The immunofiltration assay of claim 23, wherein said polystyrene/Dextran nano-particles are in aqueous phase, and wherein said assay includes a filter, on which there is at least one defined zone on that a complementary binding partner protein to a protein on said polystyrene/Dextron nano-particles is immobilized.
The immunofiltration assay works by adding theliquid sample to said filter and said sample permeates said filter, said aqueous polystyrene/Dextran nano-particles coated with proteins are then added to said filter, said polystyrene/Dextran nano-particles are thus detected in the defined zone(s), and the analyte in said sample are therefore detected qualitatively and/or quantitatively.
The immunofiltration assay works by adding theliquid sample to said filter and said sample permeates said filter, said aqueous polystyrene/Dextran nano-particles coated with proteins are then added to said filter, said polystyrene/Dextran nano-particles are thus detected in the defined zone(s), and the analyte in said sample are therefore detected qualitatively and/or quantitatively.
25. The immunofiltration assay of claim 24 wherein said defined zone(s) have line shape.
26. The immunofiltration assay of claim 24, wherein said defined zone(s) have spot shape.
27. The immunofiltration assay of claim 24, wherein said defined zones have spot shapes, forming a spot matrix.
28. The method of claims 1, 2, 3, 4, 5, 6, and 7 wherein said protein coated to said polystyrene/Dextran nano-particle is labeled with enzyme, fluorescein, biotin, isotope, magnetic material, and/or luminescent material.
29. The assay of claims 8, 9, 10, 11, 12, 13, wherein said protein coated to said polystyrene/Dextran nano-particle is labeled with enzyme, fluorescein, biotin, isotope, magnetic material, and/or luminescent material.
30. The immunochromatography assay of claims 14, 15, 16, 17, 18, 19, 20 and 21 wherein said protein coated to said polystyrene/Dextran nano-particles is labeled with enzyme, fluorescein, bitin, isotope, magnetic material, and/or luminescent material.
31. The immunofiltration assay of claims 22, 23, 24, 25, 26 and 27 wherein said protein coated to said polystyrene/Dextran nano-particles is labeled with enzyme;
fluorescein, biotin, isotope, magnetic material, and/or luminescent material.
fluorescein, biotin, isotope, magnetic material, and/or luminescent material.
32. A method for immunoassay, which employs polystyrene/Dextran nano-particles in the size of 5nm to 300nm, coated with protein such as antigen or antibody, wherein said protein is immobilized to a solid support.
33. A method for immuno-separation, which employs polystyrene/Dextran nano-particles in the size of 5nm to 300nm, coated with proteins such as antigen or antibody, wherein said protein is labeled by magnetic material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2489105 CA2489105A1 (en) | 2004-12-20 | 2004-12-20 | Development of a method for employing polystyrene/dextran nabo-particles in immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA 2489105 CA2489105A1 (en) | 2004-12-20 | 2004-12-20 | Development of a method for employing polystyrene/dextran nabo-particles in immunoassay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2489105A1 true CA2489105A1 (en) | 2006-06-20 |
Family
ID=36636679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2489105 Abandoned CA2489105A1 (en) | 2004-12-20 | 2004-12-20 | Development of a method for employing polystyrene/dextran nabo-particles in immunoassay |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2489105A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109001464A (en) * | 2018-07-09 | 2018-12-14 | 广州华澳生物科技有限公司 | A kind of marker of inflammation joint quantitative testing test paper and preparation method thereof |
-
2004
- 2004-12-20 CA CA 2489105 patent/CA2489105A1/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109001464A (en) * | 2018-07-09 | 2018-12-14 | 广州华澳生物科技有限公司 | A kind of marker of inflammation joint quantitative testing test paper and preparation method thereof |
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