CA2484270C - Cosmetic use of botulinum toxin for treatment of downturned mouth - Google Patents

Abstract

This invention provides the use of Botulinum toxin (BTX) to cause paralys is of a depressor anguli oris (DAO) musculature in a patient to alleviate downturn at corners of the patient's mouth.

Description

COSMETIC USE OF BO7'ULINUM TOXIN FOR

5= FIELD OF THE PP/Ell-MON

This invention relates to cosmetic uses of neuroparalytic toxins.
BACKGROUND OF TIIE INVENTION

Marionette lines are cosmetic defects of the human face often caused by a loss of derrnal collagen in the lower lips and chin area as a result of aging.
These lines are usually accompanied by a downturn at the corners of the mouth, producing a"sadA appearance, hence the term "sad mouth". In this condition, the horizontal symmetry of the mouth is offset in a downward or inferior directzon as a result of the downturn at the corners of the mouth.

Some facial wrinkles and unsightly facial expressions are due to overactivity of the underlying facial musculature. Neuroparalytic toxins have been used for treatment of wrinkles and in other treatments for facial rejuvenation. A toxin capable of bloclcing neuromuscular activity is administered to a facial muscle responsible for the facial defect or lesion. Resulting paralysis of the facial muscle alleviates the facial defect. The preferred toxin for cosmetic use is Botulinum toxin (BTX).
BTX, produced by the bacterium Clostridlum botulinurn reversibly paralyzes striated muscle when administered in sub-lethal doses. BTX has been used in the treatment in a number of neuromuscular disorders and conditions involving muscular spasm including various forms of dystonia, hemifacial spasm, tremor, spasticity (e.g. resulting from Multiple sclerosis), anal fismres and various ophthalmologic conditions (c.f. A. Carruthers et at (1996), Botulinum A
Exotoxin

-2-Use in Clinical Dermatology; Journal of the American Academy of Dermatology 34: 788-797).

BTX is a generic term covering a family of toxins produced by C. botulinum comprising up to eight serologically distinct forms (A, B, C,, C2, D, E, F and G).
These toxins which are among the most powerfiul neuroparalytic agents known (c.f. Melling, J. et al (1988) Clostridium Botulinum: Nature and Preparation for Clinical Use; Eye 2: 16-23). Serotypes A, B and F are the most potent. The mode of action is to inhibit the release of acetylcholine by the presynaptic nerve.
BTX-A serotyn is available commercially under the trademarks BOTOXTm (Allergan, Inc., Irvine, California, U.S.A.) and DYSPORT' (Speywood Phannaceuticals, Ltd., Maidenhead, U.K.). The initial cosmetic use of BTX was for treatment of forehead frown lines as reported in J. Carruthers and A.
Carrttthers (1992) "Treatment of G1abeBar Frown I.ines witb C. Botnlinum A Exotoxin";
J. Dermatol. Surg. Oncol. 18: 17-21. Subsequently, various facial treatments employing BTX have been reported but use of BTX for treatment of midfacial defects has been limited.

Application of BTX near the mouth has been limited to treatment of neuromuscular disorder. For example, hemifacial spasm has been treated by BTX
injection to the zygomaticus muscle but the modeolus adjacent the corner of the mouth is avoided (J. Carruthers and A. Carruthers (1996) Bomlimun A Exotoxin in Clinical Ophthalmology; Can. J. Ophthalmol. 31: 389-400).

It has been reported that BTX injection to a group of muscles on one side of a patient's face has been used to treat facial synicfnesis and vertical asymmetry caused by facial nerve palsy (Armstrong, M.W.J. et al. (1996) "Treatment of Facial Synkinesis and Facial Asymmetry with Botulinum Toxin Type A Following Nerve Palsy", Clin. Otolaryngol. 21:15-20). In the latter procedure, the levator anguli

-3-oris, zygoinaticus major, rizorius and depressor anguli oris muscles associated with the mouth together with various muscles associated with the eye on the normal side of a patient's face were all treated as a group in order to affect the entire vertical symmetry of a patient's face to compensate for effects of nerve palsy on the untreated side of the face.

While BTX treatment of the platysma muscle has -been performed for treatment of neck lines and banding, it has also been noted that injection of BTX
into the platysma produces an uplift of the mouth (F.S. Brandt and B. Bellman (1998) Cosmetic Use of Botulinum A Exotoxin for the Aging Neck; Dermatol.
Surg. 24: 1232-1234). Injection of BTX into the point of the 'chin has also been done for treatment of prominent mental crease (A. Carruthers and J.
Carruthers;
"Cosmetic Uses of Botulinum A Exotoxin"; In: James, W.D.. et al Eds. Advances in Dermatology (1997) Mosby-Yearbook, Chicago: at pages 325-48).

The inventors have now found that "sad mouth" may be treated by simultaneous bilateral BTX injection to depressor anguli oris (triangularis) muscle (termed herein DAO) thereby affectizig the horizontal symmetry of the mouth, without embarrassment to the appearance and function of the mouth. The normal function of the patient's lips is not impeded.

This invention provides the use of Botulinum toxin (BTX) to cause paralysis of a depressor anguli oris (DAO) musculature in a patient to alleviate downturn at corners of the patient's mouth.

This invention provides Aimthod of alleviating downtura of corners of a patient's mouth comprising:

a) locating a depressor anguli oris (DAO) muscle adjacent each corner of said mouth; and b) injecting into a DAO adjacent each corner of the mouth, a quantity of Botulinum toxin (BTX) sufficient to cause paralysis of a DAO.

In this invention, BTX is simultaneously injected into each DAO adjacent each corner of the patient's mouth. By simultaneously, it is meant that the injection into each DAO occurs as part of the same treatment, although a DAO on one side of the mouth may be selected for injection before the other DAO during a treatment session.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1: is a frontal view showing musculature of the human face and neck.

Figure 2: is a frontal view of a human face and neck showing the general location of the DAO muscles and sites for BTX
injection according to this invention.

The term "BTX" as used herein includes any neurotoxin produced by C. botulinum or derivatives thereof. Preferably, the neurotoxin will be the Botulinum A exotoxin, termed herein "BTX-A".

The term "Unit equivalents" as used herein, is an amount of BTX which is equivalent to staniarcl Units of BTX-A. A standard Unit of BTX-A is defined as the mean L..D50 for female Swiss Webster mice weighing 18-20 grams (E.J. Schantz and D.A. Kaultner (1978) Standardized Assay for Clostridium botulinum Toxins;
J. Assoc. Anal. Chem. 61: 96-99). The estimated human LD50 for a 70-kg person is 40 Units/kg or about 2500-3000 Units.

BOTOXTM is sold in 100 Unit vials. DYSPC3RTTM is sold in 500 Unit vials.
For cosmetic uses, the vial contents are typically diluted i or 2 ml of with sterile saline solution, which for BOTOV' provides a 100 or 50 Unitlml dilution.
DYSPORTTm BTX-A is rougWy tenfold less toxic than BOTOXTm and approxzrnately fourfold greater amounts of the DYSPORT' product will usually be injected to achieve the same result as would is obtained using a specific number of Units of BOTOX"m.

Commercially available toxin is typically freeze dried and is stored froxen (e.g. at -4 C) until ready for use. The toxin is diluted just prior to use.
The resulting solution should be used within several hours of preparation. Care should be taken to avoid foaming of the solution by slowing the entry of saline into the vacuum of the storage vial. The solution should not be shaken. Once used, remaining toxin, vial, needles and syringes should be disposed of in a manner appropriate for biohazardous waste.

Typical injection technique involves the use of a short, narrow needle (e.g. ih inch or 8mnn; 30-gage) with an insulin or taberculzn type syringe.
Patients are typicaily treated in the seated position. The skin area is cleaned with an alcohol swab. A single syringe may be used for multiple injections to treat different locations in a single muscle or different locations on a patient's face.
Typically, the plunger of the syringe is depressed as the needle is withdrawn so that toxin is evenly distributed at the injection site. Pressure or gentle massage may be applied at the injection site to assist in dissipating the toxin. The toxin wiIl typically migrate approximately 1 cm from the site of injection.

= CA 02484270 2005-03-23 =

Electromyographic (EMG) guided needles may be used for injection to determine needle location of a high de;;ree of accuracy, although this technique is generally not necessary nor is it required in the instant invention.

In prior applications of BTX, i:otal dose per treatment is variable and is largely dependent upon the condition being treated and the site of application of BTX. For example, a total dose of 20-30 Units will typically be applied to the glabellar complex and 60-75 Units for platysmal bands (c.f. A. Carruthers and J. Carruthers (1998) History of the Cosmetic Use of Botulinum A Exotoxin;
Dermatol. Surg. 24: 1168-1170). Doses of up to 200 Units per treatment session for the platysma have been reported (Brandt and Bellman [supra]). In prior applications, a typical dose at a single injection point is approximately 5 Units of toxin in a 100 Unitlml dilution.

Onset of muscle paralysis follow:ing injection usually occurs within hours of treatment. The duration of paralysis will vary from patient to patient.
Typically, duration will be from 2-8 months before subsequent treatment is required to alleviate the condition.

This invention provides a succes:,ful treatment of Marionette lines and "sad mouth" by BTX injection into the DAO. In such treatment, the orbicularis oris muscle surrounding the mouth is normally avoided in view of its sensitivity to BTX.
However, the orbicularis oris muscle may be treated in conjunction with this invention to alleviate severe upper lip lines or wrinkles. In such a case, a small amount of BTX is injected into the orbicularis oris, with the total dose being in the order of 4 Units for the entire upper lip. In general, care must be used in BTX
treatments close to the mouth because of i_he danger of producing a flaccid cheek, an incompetent mouth, or an asymmetric smile.

The DAO may be found by instructing the patient to voluntarily and forcibly pull down the corners of the mouth. The DAO can be then felt by pulling inferiorly at a point approximately 1 c3n lateral and 8 nun inferior to the commissure.
Alternatively, EMG localization may be performed.
Treatment should take into consideration the pre-existing symmetry bf the mouth and is performed on both sides of the' face in order to provide a symmetric result. The required dosage to each side of the mouth should be judged and if necessary, altered upon re-treatment.
Injection may be made into any part of the DAO musculature. The injection is intramuscular and may be performed using the injection techniques used for other BTX treatments as descn'bed above. On a typical patient's face, the preferred point of injection into the DAO will be approximately 7 mm laterally and 8 mm.
inferior from the corner of the mouth. A single injection witl usually suff'ice with the dosage for a single DAO muscle ranging from 2#101) Units - 5(f109b) iTnits. Tlie best dose will depend upon the sex of the patient and size of the individual.
Typical doses for a female will be 2( 10%) Units - 3(f10%) Units for one side of the mouth; and, for a male, 3(t10gb) Units .- 5 (t10%) Units for'one side of the mouth.

Contraindications for the use of BTX are known in the art and include children under 12, pregnancy, lactation, history of . neuromuscular disease and known sensitivity to BTX or human albumin. This invention is not be recommended for patients who are singers, musicians and other individuals who use their perioral muscles with intensity. However, treatment according to this invention will normally not affect normal, speech, whistling or mastication particularly in cases where the orbicularis oris is not treated.

Follawing application of this invention, laser resurfacing techniques may be employed to enhance the effect. In the . case of very deep - Marionette lines, performance of this invention may be followed by soft tissue filling treatment such as collagen, Hylaform", Restlyanel (soft forrn) or Gore Tex7 implants.

The drawings may be referred to for illustration of performance af this invention. Figure 1 shows the location of various facial muscles and anatomical locations referred to herein, including: the DAO 1; orbicularis oris 2;
zygomaticus 3; and, the modeolus 4. The platysma, which overlies the neck muscles and extends in part, to region 5, is not illustrated in Figure 1.

In Figure 2, region(s) 6 bounded by dashed lines illustrate the general location on the patient's skin which overly the DAO. Suitable injection sites include the areas within region(s) 6 that are marked with a "X".

While the following claims are intended to recite the features of the invention, it will be apparent to those of skill in the art that certain changes may be made without departing from the scope of this invention.

Claims (6)

We claim:

1. Use of a Botulinum toxin to cause paralysis of an orbicularis oris muscle in proximity to an upper lip of a human afflicted with severe upper lip wrinkles, for alleviating the severe upper lip wrinkles.

2. Use of claim 1, wherein the Botulinum toxin is a Botulinum-A toxin.

3. Use of 2 to 4 units of a Botulinum toxin to cause paralysis of an orbicularis oris muscle in proximity to an upper lip of a human afflicted with severe upper lip wrinkles, for alleviating the severe upper lip wrinkles.

4. Use of 3 to 4 units of a Botulinum toxin to cause paralysis of an orbicularis oris muscle in proximity to an upper lip of a human afflicted with severe upper lip wrinkles, for alleviating the severe upper lip wrinkles.

5. Use of 4 units of a Botulinum toxin to cause paralysis of an orbicularis oris muscle in proximity to an upper lip of a human afflicted with severe upper lip wrinkles, for alleviating the severe upper lip wrinkles.

6. The use of claim 1, followed by laser resurfacing tissue in proximity to a mouth and/or filling soft tissue in proximity to the mouth.