CA2483570A1 - Systeme reactif et procede pour surveiller l'adenosine triphosphate - Google Patents

Systeme reactif et procede pour surveiller l'adenosine triphosphate Download PDF

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Publication number
CA2483570A1
CA2483570A1 CA 2483570 CA2483570A CA2483570A1 CA 2483570 A1 CA2483570 A1 CA 2483570A1 CA 2483570 CA2483570 CA 2483570 CA 2483570 A CA2483570 A CA 2483570A CA 2483570 A1 CA2483570 A1 CA 2483570A1
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Canada
Prior art keywords
reagent
atp
sample
concentration
reagent system
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2483570
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English (en)
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CA2483570C (fr
Inventor
James E. Cairns
Phillip J. Whalen
Patrick A. Whalen
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Luminultra Technologies Ltd
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Luminultra Technologies Ltd
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Priority to CA2483570A priority Critical patent/CA2483570C/fr
Publication of CA2483570A1 publication Critical patent/CA2483570A1/fr
Application granted granted Critical
Publication of CA2483570C publication Critical patent/CA2483570C/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un système d'agents réactifs comprend un premier réactif qui comporte un tampon phosphate à pH élevé et un deuxième réactif qui comporte de la luciférase, de la luciférine, un sel de magnésium et un stabilisateur denzyme. Le deuxième réactif présente un pH bas et un tampon ayant un pK qui est proche du pH optimal pour une activité de luciférase. Le système dagents réactifs peut être utilisé dans un procédé de mesure dadénosine triphosphate totale (ATP) ou d'ATP extracellulaire dissoute dans un liquide renfermant des micro-organismes. Le système dagents réactifs peut également être utilisé dans un procédé de rétablissement ou de production microbiologique.
CA2483570A 2004-10-01 2004-10-01 Systeme reactif et procede pour surveiller l'adenosine triphosphate Active CA2483570C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA2483570A CA2483570C (fr) 2004-10-01 2004-10-01 Systeme reactif et procede pour surveiller l'adenosine triphosphate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA2483570A CA2483570C (fr) 2004-10-01 2004-10-01 Systeme reactif et procede pour surveiller l'adenosine triphosphate

Publications (2)

Publication Number Publication Date
CA2483570A1 true CA2483570A1 (fr) 2006-04-01
CA2483570C CA2483570C (fr) 2016-06-28

Family

ID=36121746

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2483570A Active CA2483570C (fr) 2004-10-01 2004-10-01 Systeme reactif et procede pour surveiller l'adenosine triphosphate

Country Status (1)

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CA (1) CA2483570C (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113846141A (zh) * 2021-07-26 2021-12-28 浙江工商大学 基于乳液界面能诱导释放的生理毒性快速检测方法及应用
CN114672536A (zh) * 2022-01-07 2022-06-28 中国科学院生态环境研究中心 一种基于铝盐混凝预处理的水中病毒含量的检测方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113846141A (zh) * 2021-07-26 2021-12-28 浙江工商大学 基于乳液界面能诱导释放的生理毒性快速检测方法及应用
CN114672536A (zh) * 2022-01-07 2022-06-28 中国科学院生态环境研究中心 一种基于铝盐混凝预处理的水中病毒含量的检测方法
CN114672536B (zh) * 2022-01-07 2023-10-13 中国科学院生态环境研究中心 一种基于铝盐混凝预处理的水中病毒含量的检测方法

Also Published As

Publication number Publication date
CA2483570C (fr) 2016-06-28

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