CA2482761C - Activation of equine oocytes - Google Patents

Activation of equine oocytes Download PDF

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Publication number
CA2482761C
CA2482761C CA2482761A CA2482761A CA2482761C CA 2482761 C CA2482761 C CA 2482761C CA 2482761 A CA2482761 A CA 2482761A CA 2482761 A CA2482761 A CA 2482761A CA 2482761 C CA2482761 C CA 2482761C
Authority
CA
Canada
Prior art keywords
activation
calcium
oocyte
medium
oocytes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2482761A
Other languages
English (en)
French (fr)
Other versions
CA2482761A1 (en
Inventor
Gordon L. Woods
Dirk K. Vanderwall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Idaho Research Foundation Inc
Original Assignee
Idaho Research Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Idaho Research Foundation Inc filed Critical Idaho Research Foundation Inc
Publication of CA2482761A1 publication Critical patent/CA2482761A1/en
Application granted granted Critical
Publication of CA2482761C publication Critical patent/CA2482761C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer
CA2482761A 2002-04-18 2003-04-09 Activation of equine oocytes Expired - Fee Related CA2482761C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US37403202P 2002-04-18 2002-04-18
US60/374,032 2002-04-18
PCT/US2003/011355 WO2003089578A2 (en) 2002-04-18 2003-04-09 Activation of equine oocytes

Publications (2)

Publication Number Publication Date
CA2482761A1 CA2482761A1 (en) 2003-10-30
CA2482761C true CA2482761C (en) 2011-03-22

Family

ID=29251124

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2482761A Expired - Fee Related CA2482761C (en) 2002-04-18 2003-04-09 Activation of equine oocytes

Country Status (5)

Country Link
US (1) US7323620B2 (de)
EP (1) EP1501350A4 (de)
AU (1) AU2003232003B2 (de)
CA (1) CA2482761C (de)
WO (1) WO2003089578A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7689559B2 (en) * 2006-02-08 2010-03-30 Telenor Asa Document similarity scoring and ranking method, device and computer program product
WO2015081182A1 (en) 2013-11-27 2015-06-04 The Curators Of The University Of Missouri Artificial oocyte activation
CN111944744B (zh) * 2020-07-10 2023-07-18 成都艾伟孚生物科技有限公司 一种卵母细胞激活液及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496720A (en) * 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
EP1149898A2 (de) * 1993-12-23 2001-10-31 Infigen, Inc. Embryonale Stammzellen von Huftieren als Kerndonatoren und Kerntransfertechniken zur Herstellung chimärer und transgener Tiere
US5400799A (en) * 1994-01-11 1995-03-28 Yoches; Cheri C. Method of monitoring uterine activity in veterinary obstetrics
US5945377A (en) * 1996-12-06 1999-08-31 Board Of Trustees Operating Michigan State University Compositions containing herbicide and monosaccharides and method of use thereof
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells

Also Published As

Publication number Publication date
EP1501350A4 (de) 2006-08-30
AU2003232003B2 (en) 2006-05-11
EP1501350A2 (de) 2005-02-02
US20030200556A1 (en) 2003-10-23
CA2482761A1 (en) 2003-10-30
US7323620B2 (en) 2008-01-29
WO2003089578A2 (en) 2003-10-30
WO2003089578A3 (en) 2004-02-26
AU2003232003A1 (en) 2003-11-03

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Effective date: 20150409