CA2473656A1 - Microcentrifuge plunger system - Google Patents

Microcentrifuge plunger system Download PDF

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Publication number
CA2473656A1
CA2473656A1 CA 2473656 CA2473656A CA2473656A1 CA 2473656 A1 CA2473656 A1 CA 2473656A1 CA 2473656 CA2473656 CA 2473656 CA 2473656 A CA2473656 A CA 2473656A CA 2473656 A1 CA2473656 A1 CA 2473656A1
Authority
CA
Canada
Prior art keywords
shaft
microcentrifuge tube
cap
plunger
piston
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2473656
Other languages
French (fr)
Inventor
Dave Houtman
Melissa Sheldrick
Ruba Vettivel
Kowsala Kandasamy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CA 2473656 priority Critical patent/CA2473656A1/en
Publication of CA2473656A1 publication Critical patent/CA2473656A1/en
Abandoned legal-status Critical Current

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0289Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids

Abstract

The present invention relates to a tapered plunger designed to dispense viscous material from a microcentrifuge tube such as is commonly found. in a laboratory setting. Material is extruded through a hole made by the user in the tip of the microcentrifuge tube.
The plunger incorporates a calibrated shaft and is capable of delivering uniform quantities of material as the plunger passes through a snap-on cap. A cap is designed to form a tight seal with the rim of the microcentrifuge tube, preventing material from being extruded through the top of the tube, and holding the plunger in place, colinear with the tube.

Description

MICROCENTRIFUGE PLUNGER SYSTEM
BACKGROUND OF THE IN~J'ENTION
The present invention. relates to a manually operable device for dispensing viscous material from a microcentrifuge tube.
The apparatus and method of operation of the present invention are suitable for use in scientific laboratories in which procedures may require the transfer of small volumes of viscous material.
Microcentrifuge tubes are used in a wide: variety of laboratory procedures, and the material enclosed within the tube is typically transferred in a quantifiable manner by the use of a micropipette. This device makes use of negative pressure to remove material from the open top of the microcentrifuge tube. Although this praca zce has been shown to be both efficient and accurate, the use of micropipettes becomes an impediment when attempting to transfer or remove viscous material. A common problem encountered in protocols that require the quantifiable transfer of viscous material from a microcentrifuge tube is inaccuracy due to inefficient removal of the viscous _2_ material and hence loss of small volumes of perhaps expensive material on the walls of the microcentrifuge tube and on the exterior of the pipette tip itself.
Furthermore, as the number of different surfaces with which the material makes contact increases, so does the possibility of contamination of the material during transfer or extrusion. Thus the use of a micropipette adds an additional surface with which the material. may be contaminated by. The preferred embodiment of the present invention eliminates this additional step by effectively converting the microcentrifuge tube into the barrel of a syringe.
The above-mentioned problems illustrate the need for an improved device for the transfer of viscous material that allows for both efficient and accurate transfer from a microcentrifuge tube while also minimizing contamination.
The apparent benefits of this device are further amplified when lab protocols require that material be centrifuged into the tip of the tube prior to further manipulation. Use of this device ensures that the material can be mixed, centrifuged and extruded from one common container, minimizing handling and contamination, while further reducing the possibility of mateo~ial loss at each step.
SUMMARY OF THE INVENTION
The proposed invention comprises a cap which. is modelled to replace the cap of a typical .rnicrocentrifuge tube with a combination of cap and plunger unit. A
manually operated plunger through the cap may be used to exert positive pressure on the viscous material inside of the tube thereby allocating removal of this material from an opening in the tip of the microcentrifuge tube, rather than via the open top of t~-~e tube .
It is easy and simple to operate this mechanical device; the cap of the present invention fastens securely to the open rim of a standard mierocentrifuge tube and the shaft may be operated manually by applying pressure to a button which is an external component of the plunger. Each position of the shaft will indicate the amount of material being dispensed. Prior to use the user must make a small hole in the tapered. base of the micracentrifuge tube through which. materia3. will be extruded.

DRIEF DESCRIPTION OF THE DRAWINGS
Figure la is the preferred embodiment of a device of the present invention.
Figure lb is a cross section illust:ratin.g the orientation of the de-ice in position relative to an attached microcentrifuge tube (8).
Figure 2 shows the plunger, comprising the button (1), the shaft (6) and the piston (10) .
Figure 3a shows a. side view of the cap without the shaft .
Figure 3b indicates a top view of t:he ca.p with a circular aperture in the cap through whiwh the shaft passes perpendicular to the plane of the page.
Figure 3c indicates an alternative aspect of the cap in which the shaft is in the form of a cross in its cross-sectional area.
Figure 4a shows s. partial cross-section view of the preferred embodiment of the cap and its :relationship to the matching elements of the shaft.
Figure 4b shows a partial cross-section view indicating the displacement of equal vol~.zmes of material as a consequence of the operation of the device as the plunger translates through the calibration mechanism of the cap.
Figure 5 indicates the device of Figure 1a with the assembled apparatus ire actual use, with an attached microcentrifuge tubeo DETAILED DESCRIPTID~
As discussed above, devices of the ;presEnt invention are suitable for use i.n a laboratory environment wherein protocols may require the transfer or extrusion of viscous material. Devices of the present invention s.re well suited for application of materials having a wide range of VISCOS3tles.
Referring to figure l.b, in a preferred embodiment of the present invention, the apparatus comprises a manually operable device for dispensing viscous material through a opening (13) made by the user in the tip (l~) of a microcentrifuge tube (8). The apparatus comprises a device, previously and henceforth referred to as a plunger, designed to move colinearly with the longitwdinal axis of the microcentrifuge tube. The piston (10;1 of the plunger mates with the inner surface of the tip (15) of the microcentrifuge tube when the plunger is at i.ts farthest extent of travel in the microcewtrifuge tube.
In a preferred embodiment, the plunger ~s shown in Figure 2 includes a tapered piston (10), longitudinal shaft (6) and external button (1), wherein the shaft (6) may be of any cross section capable of providing a structurally supportive connection to the piston.
Tn the preferred method of use, a hole is created, for example, by opening the tip (16) of the ~icrocentrifuge tube ( 8 ) at a suitable location ( 13 ) sucai that the inner surface of the tip (15) forms an opening through which material may be extruded by the application of pressure on the button (1). The precise location of the created opening (13) will bear no effect on the amount of material extruded during the actual operation of 'the c~.evice.
~-iowever, a small amount of material may .~_nitially be lost in the manufacture of such an opening. Total material losses will be minimized by ensuring that the location of the opening (13) lies close to the inner surface of the tip (15) .
_7_ Referring to Figure lb~ the plunger moves longitudinally along the cylindrical barrel of a microcentrifuge tube !; 8 ) .
Referring to Figures 2, the button (1) comprises a flat platform (21), for example disc-shaped, whose planar surface dimensions exceed those of the cross--section of the shaft (6) and is oriented perpendicular to the shaft.
Referring to figure lb, the button (I) trans~_ates force applied by the user to the piston (10) via a shaft (6) running parallel to the long axis of the dev~_ce and microcentrifuge tube.
Referring to Figure Ib, the plunger comprises a tapered piston (10) capable of adapting to irregularities in the inner surface of a standard microcentrifuge tube.
In the ideal embodiment, the piston (10) is made of a deformable material such that the effects of the above-mentioned irregularities on total extruded volume can be minimized.
Referring to figure lb, the point of contact made by the plunger and the ir~ner walls of the microc:entrifuge tube (9) are tight enough to exclude the extrusion of the viscous material beyond the top of the pistor~ where it _g_ contacts with the walls (9), but permits the release of air from the chamber formed between the pist=.on (10) and the inner surface of the tip ( 15 ) of the microcentrifuge tube s To establish tight contact (9) between the inner surface of the microcentrifuge tube (8) and the piston (10), the portion of the piston that immediately contacts the base of the shaft (18) has an outer surface which mates with the cylindrical :portion (lf) of the m=icrocentrifuge tubee A cap (indicated in figure 3a) com~>rise:s an inner flared Contact portion (4), an outer flange (19) and an external lip (20) . R.eferring to figure lb, 'the cap is designed to contact t~2e rim (3) of the microcentrifuge tube (8) and form a tight seal with it, for example, via a flared contact portion (4). The outer contact flange (19) of the cap exterior to the microcentrifu.ge tube when the device is in use, is wider than the inner diameter of the microcentrifuge tube so as to secure the cap and to prevent its displacement into the barrel of the microcentrifuge tubee In the preferred embodiment of the cap, an external lip (20) is provided to facilitate manual removal of the inner flared contact ;4) of the cap from within the body of _g_ the microcentrifuge tube when attached for use in normal operation.
Referring to figure 3b, the cap has a central portion defining an opening (22), through which the shaft (6) of the plunger passes perpendicular to the flat surface of the cap. This arrangement prevents the unwanted extrusion of material through the natural opening of the microcentrifuge tube (8) while allowing the piston (10) to communicate with the button (1) via the adjoining shaft (6).
Referring to figures 1b, 3b and 4a, upon attachment of the cap to the microcentrifuge tube (8), the plunger is held colinear with the longitudinal axis of t~.he microcentrifuge tube ar.~d the button (1) remains external to the open end of the microcentrifuge tube. v~_a this arrangement the shaft (~) of the plunger is capable of moving longitudinally through the opening of the cap (22) such that the piston i;10) may contact the inner surface of the tip (15) of the microcentrifuge tube.
The shaft (6) of the device is composed of an stiff material which, in the ideal embodiment, does not flex appreciably when the apparatus is in use, thereby ensuring that changes in the lcongitudinal pos.i.tio:n. of the button (1) are accurately translated in the motion of the piston (10).
In the ideal embodiment, the shaft (~) flares to provide additional structural support at: the point (18) where the shaft (6) contacts the piston (1) .
Referring to figure 4a, the shaft (6) is calibrated longitudinally via calibration elements of t:he shaft (7) at regular intervals over the functional length of travel of the shaft through the cap. The opening (22) through which the shaft (6) passes through the cap comprises matching elements (24) that mate with the calibration elements of the shaft (7). Of the many possible embodiments of this mechanism, one example is of a series of cop~_anar grooves that make contact with complimentary matching elements (5) in the opening (22) of the cap. According to another aspect (refer to figure 3c), an alternate version of the opening (22) may be used to mate with the calibration elements of the shaft (6) wherein the shaft i.s of a different cross-sectional structure.
Refering to figures lb and Via, to facilitate removal of the plunger via the use of the external cap, a portion of the shaft remains external to the cap, such that upon full insertion of the plunger into the microcentrifuge tube (8) space remains between the top of the cap (19) and the button (1) to help facilitate eectraction of the plunger from the microcentrifuge tube following standard use.
An external lip (20} of the cap extends beyond the rim of the microcentrifuge tube~ to facilitate removal of the flared contact portion of the cap (4) from within the microcentrifuge tube (8}.

Claims (19)

1. A manually operable device for dispensing viscous material through the base of a microcentrifuge tube, comprising a plunger designed to mate with the inner surface of the microcentrifuge tube.
2. An apparatus as defined in claim 1, wherein viscous material is extruded through a hole made by the user in the tip of the microcentrifuge tube.
3. An apparatus as defined in claim 2, wherein the plunger comprises a piston, longitudinal shaft and external button.
4. An apparatus as defined in claim 3, whereby the plunger runs longitudinally along the barrel of a microcentrifuge tube.
5. An apparatus as defined in claim 4, wherein the button comprises a flat platform whose planar surface dimensions exceed those of the cross-section of the shaft and is oriented perpendicular to the shaft.
-13-~
6. An apparatus as defined in claim 4, wherein the piston comprises a tapered deformable tip capable of adapting to irregularities in the inner surface of a microcentrifuge tube.
7. An apparatus as defined in claim 4, wherein the portion of the piston that immediately contacts the base of the shaft has an outer surface which mates with the cylindrical portion of the microcentrifuge tube.
8. An apparatus as defined in claim 2, wherein a cap is designed to contact the rim of the microcentrifuge tube and form a tight seal with it.
9. An apparatus as defined in claim 8, wherein the cap has a central portion defining anopening.
10. An apparatus as defined in claims 7 and 8, wherein the shaft of the plunger passes perpendicularly through the planar surface of the cap.
11. An apparatus as defined in claim 8, wherein upon attachment of the cap to the microcentrifuge tube, the plunger is held colinear to the longitudinal axis of the microcentrifuge tube and the button remains external to the open end of the microcentrifuge tube.
12. An apparatus as defined in claim 9, whereby the shaft of the plunger is capable of moving longitudinally through the opening of the cap such that the piston may contact the inner surface of the tip of the microcentrifuge tube.
13. An apparatus as defined in claim 4, wherein the shaft is composed of a rigid material that does not flex under pressure when the apparatus is in use.
14. An apparatus as defined in claim 13, wherein the shaft flares where it joins with the piston.
15. An apparatus as defined in claim 4, wherein additional structural support is included at the point where the shaft contacts the piston.
16. An apparatus as defined in claim 12, wherein the shaft is calibrated longitudinally at regular intervals over the functional length of travel of the shaft through the cap.
17. An apparatus as defined in claim 9, wherein the opening through which the shaft passes through the cap comprises matching elements that mate with the calibration elements of the shaft.
18. An apparatus as defined in claim 4, wherein upon full insertion of the plunger into the microcentrifuge tube, space remains between the top of the cap and the button of the plunger.
19. An apparatus as defined in claim 8, wherein the external plate of the cap extends beyond the rim of the microcentrifuge tube.
CA 2473656 2004-08-16 2004-08-16 Microcentrifuge plunger system Abandoned CA2473656A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2473656 CA2473656A1 (en) 2004-08-16 2004-08-16 Microcentrifuge plunger system

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2473656 CA2473656A1 (en) 2004-08-16 2004-08-16 Microcentrifuge plunger system

Publications (1)

Publication Number Publication Date
CA2473656A1 true CA2473656A1 (en) 2006-02-16

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2473656 Abandoned CA2473656A1 (en) 2004-08-16 2004-08-16 Microcentrifuge plunger system

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9688458B1 (en) 2013-05-31 2017-06-27 Hasbro, Inc. Folding portable craft gun with storage for plunger rod
CN108939629A (en) * 2018-09-30 2018-12-07 重庆江北机械有限责任公司 A kind of shuttle valve discharging tubular type solid-liquid separating machine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9688458B1 (en) 2013-05-31 2017-06-27 Hasbro, Inc. Folding portable craft gun with storage for plunger rod
CN108939629A (en) * 2018-09-30 2018-12-07 重庆江北机械有限责任公司 A kind of shuttle valve discharging tubular type solid-liquid separating machine

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