CA2470670A1 - Tnf ligands - Google Patents

Abstract

Ligands to a member of the TNF/NGF receptor family are provided. The ligands bind to the region of the C-terminal cysteine loop of such a receptor. A process for the preparation of the ligands is also provided, as well as pharmaceutical compositions comprising the ligands.

Description

r t TNF LIGANDS
This application is a divisional application of the co-pending application 2,105,534, filed September 3, 1993.
FIELD OF THE INVENTION
The present invention relates to ligands to Tumor Necrosis Factor receptors (TNF-Rs) which inhibit the effect of TNF but not its binding to the TNF-Rs, as well as to ligands.interacting with other receptors of the TNF/NGF receptor family.
. H~ACKGROUND OF ~~NTION
Tumor necrosis factor (TNF) is a pleiotropic cytokine, produced by a number of cel-1 types, mainly by activated macrophages. It is one of the principal mediators of the immune and inflammatory response. Interest in its function has greatly increased, recently, in view of evidence of the involvement of TNF in the pathogenesis of a wide range of disease states, including endotoxin shock, cerebral malaria and graft-versus-host reaction. Since many of the effects of _TNF are deleterious to . the organism, it is of great interest to find ways of blocking its action on host cells. An evident target for such intervention are the molecules. to which TNF has to bind in order to exert its effects, namely the TNF-Rs. These molecules exist not only in cell-bound, but also in soluble forms, consisting of the cleaved extra-cellular domains of the intact receptors (see Nophar et al., EMBO Journal, ,~t10):3269-78, 1990). The soluble receptors maintain the ability to bind TNF, and thus have the ability to block its function by competition with surface receptors.

Another method of TNF inhibition based on the principle of competing with cell-bound molecules, is the use of antibodies recognizing TNF receptors and blocking the ligand binding.
The cell surface TNF-Rs are expressed in almost all cells of the body. The various effects of TNF, the cytoi~oxic, growth-promoting and others, are all signalled by the TNF receptors upon the binding of TNF to them. Two forms of these receptors, which differ in molecular size: 55 and 75 kilodaltons, have been described, and will be called herein p55 and p75 TNF-R, respectively. It should be noted, however, that there exist publications which refer to these receptors also as p60 and p80.
The TNF-Rs belong to a family of receptors which are involved in other critical biological processes. Examples of these receptors are the low affinity NGF receptor, which plays an important role in the regulation of growth and differentiation of nerve cells.
Several other receptors are involved in the regulation of lymphocyte growth, such as CDw40 and some others. Another member of the family is the FAS receptor also called APO, a receptor which is involved in signalling for apoptosis and which, based on a study with mice deficient, in its function, seems to play an important role in the etiology of a lupus-like disease. Herein, this family of receptors is called "TNF/NGF receptor family". .
One of the most striking features of TNF compared to other cytokines, thought to contribute to the pathogenesis of several diseases, is its ability-to elicit cell death... The cell-killing activity of TNF is thought to be induced by the p55 receptor.
However, this p55 receptor activity can be assisted by the p75 receptor, through a yet unknown mechanism.
European Patent Publication Nos. 0,398,327 and 0,412,486 disclose antibodies to the soluble TNF-Rs. These antibodies were found to recognize the soluble TNF-Rs and to inhibit the binding of TNF to the TNF-Rs on the cell surface. Monovalent Flab) fragments blocked the effect of TNF, while intact antibodies were observed to mimic the cytotoxic effect of TNF.
SUI~iARY OF THE INVENTION
The present invention provides a ligand to a member of the TNF/NGF
receptor family, which binds to the. region of the C-terminal cysteine loop of such a receptor. This region includes the amino acid sequence cys-163 to thr-179 in the p75 TNF-R or a corresponding region in another member of the TNF/NGF family.
Preferably, the receptor is the TNF-R, in particular the p75 TNF-R.
One such ligand includes the amino acid sequence for the CDR
region of the heavy chain of monoclonal antibody No. 32, shown in Fig. 11 or the amino acid sequence for the CDR region of the light chain of this antibody shown in Fig. 12.

Another such ligand includes the amino acid sequence for the CDR
region of the heavy chain of monoclonal antibody-no. 70 shown in Fig. 11.
Yet another such ligand includes the amino acid sequence for the CDR region of the heavy chain of monoclonal antibody no. 57, shown in Fig. 11.
The above antibodies are called herein, for simplicity's sake, "group 32" antibodies.
In another aspect of the invention, the ligands comprise the scEv of a group 32 antibody.
The ligands may comprise e.g. proteins, peptides, immunoadhesins, antibodies or other organic, compounds.
The proteins may comprise, for example, a fusion protein of the ligand with another protein, .optionally linked by a peptide.
linker. Such a fusion protein can increase the retention time of the.ligand in the body, and thus may even allow the ligand-protein complex to be employed as a latent. agent or as a vaccine.
The term "proteins" includes muteins and fused proteins, their salts, functional derivatives. and active fractions.
The peptides include peptide bond replacements and/or peptide mimetics, i.e, pseudopeptides, as known. in the art ('see e.g.

Proceedings of the 20th European Peptide Symposium, ed. G. Jung, E. Bayer, pp. 289-336, and references therein), as well as salts and pharmaceutical preparations and/or formulations which render'' the bioactive peptides) particularly suitable for oral, topical, nasal spray, ocular pulmonary, T:V. or subcutaneous delivery, depending on the particular treatment indicated. Such salts, formulations, amino acid replacements arid pseudopeptide struc-tures may be necessary and desirable to enhance the stability, formulation, deliverability (e.g. slow release, prodrugs), or to improve the economy of production, as long as they do not adversely affect the biological activity of the peptide.
Besides substitutions, three particular forms of peptide mimetic and/or analogue structures of particular relevance when desig-nating bioactive.peptides, which have to bind to a receptor while risking the degradation by proteinases and peptidases in the blood, tissues and elsewhere, may be mentioned speci.~fically, illustrated by the following examples: Firstly, the inversion of backbone chiral centres leading to D-amino acid residue structures may, particularly at the N-terminus, lead to enhanced stability for protealytical degradation without adversely affecting activity. An example .is given.iri the paper "T.ritriated D-alai-Peptide T Binding", Smith C.S. et al., Drug Development Res. 15, pp. 371-379 (1988). Secondly; cyclic. structure for stability, such as N to C interchain i.mid~es and lactames (Ede et al. in Smith and Rivier {Eds.) "Peptides: Chemi,stxy and Biolagy", Escom, Leiden {1991), pp. 268-270), and sometimes also receptor binding may be enhanced by forming cyclic: analogues. An example 1 , of this is given in "Confirmationally restricted thymopentin-like compounds", US Pat. 4,457,489 (1985), Goldstein, G. et al.
Thirdly, the introduction of ketomethylene, methylsufli:de or retroinverse bonds to replace peptide bonds, i.e, the interchange of the CO and NH moieties are likely to emhance both stability and potency. An example of this type is given in the paper ''Biologically active retroinverso analogues of thymopentin", Sisto A. et al in Rivier, J.E. and riarshall, G.R. (eds) ''Peptides, Chemistry, Structure and Biology", Escom, Leiden (1990). PP~ 722-773).
The peptides of the invention can be synthesized by various methods which are known in principle, namely by chemical coupling methods (cf. Wunsch, E: "Methoden der organi.schen Chemie", Volume 15, Band 1 + 2, Synthese von Peptiden, th:i.me Veriag, Stuttgart (1974), and Barrany, G.; Marrifield, R.B.: "The Peptides", eds.
E. Gross, J. Meienhofer, Volume 2, Chapter. l., pp. 1-284, Academic Press (1980)), or by enzymatic coupling methods (cf. Widmer, F.
Johansen, J.T., Carl.sberg Res: Gommun., Vo1.44, pg.~ 37=46 (1979), and Kullmann, W.: "Enzymatic Peptide Synth.esis'° CRC Press Inc.
Boca Raton, F1. (1987), and Widmer, F., Johansen, J..T. in "Synthetic Peptides in Biology and Medicines:, eds. Alitalo, R., Partanen, P., Vatieri, A., pp.79-86, Elsevie~r, Amsterdam {1985)), or by a combination of chemical and enaymat:ic methods if this is advantageous for the process design and economy.
A cysteine residue may be added at both t:he amino and carboxy terminals of the peptide, which will allow t;he cyclisation of the r , peptide by the formation of a di-sulphide bond.
Any modifications to the peptides of the present invention which do not result in a decrease in biological activity are within the scope of the present invention.
There are numerous examples which illustrate the ability of anti-idiotypic.antibodies (anti-Id Abs) to an antigen to function like that antigen in its interaction with animal cells and components of cells. Thus, anti-Id Abs to a peptide hormone antigen can have hormone-like activity and interact specifically with a mediator in the same way as the receptor does. {For a review of these properties see: Gaulton, G.N. and Greane, M.I.
1986. Idiotypic mimicry of biological receptors, Ann. Rev.
Immunol. Vol. 4, pp. 253-280; Sege K. and Peterson, P.A., 1978, Use of anti-idiotypic antibodies as cell surface receptor probes, Proc. Nati. Acad. Sci. U.S.A., Vol. 75, pp. 2443-2447).
It is expected from this functional similarity of anti-Id Ab and antigen, that anti-Id Abs bearing the internal image of an.
antigen can induce immunity to such an antigen. (See review in Hiernaux, J.R., 1988, Idiotypi.c vaccines and infectious diseases, Infect. Immun., Vol: 56, pp. 1407-1413).
It is therefore possible to produce anti-id~:otypic antibodies to the peptides of the present invention which will have similar biological activity.

~ , Accordingly, the present invention also provides anti-idiotypic antibodies to the peptides of the present invention, the anti-idiotypic antibody being.capable of inhibiting TNF toxicity, but not its binding to the receptor.
The individual specificity of antibodies resides in the structures of the peptide loops making up the Complementary Determining Regions (CDRs) of the variable domains of the antibodies . Since in general the amino acid sequence of the CDR
peptides of an anti-Id Ab are not identical to or even:similar to the ~ami.no acid sequence of the peptide antigen from which it was originally derived, it follows that peptides whose amino acid sequence in quite dissimilar, in certain contexts, can take up a very similar three-dimensional structure. The concept of this type of peptide, termed a "functionally equivalent sequence" or mimotope by Geyson is known. (Geyson, H.M, et al, 1987, Strategies for epitope analysis using peptide. synthesis., J.
Immun. Methods, Vol. 102, pp. 259-274).
Moreover, the three-dimensional..structure~ and function of the biologically active peptides can be simulated by other compounds, some not even peptidic in nature, but which nevertheless mimic the activity of such peptides. This fie7_d is summarized in a review by Goodman, M. (1990), (Synthesis , Spectroscopy and computer simulations in peptide research, Proc. 11th American Peptide Symposium published in Peptides-Chemistry, Structure and Biolocrv, pp. 3-29; Eds. Rivier, J.E. and Marshall, G.R. Publisher Escom).

It is also possible to produce peptide and non-peptide compounds having the same three-dimensional structure as the peptides of the present invention. These "functionally equivalent structures"
or "peptide mimics" will react with antibodies raised against the peptide of the present invention and may also be capable of inhibiting TNF toxicity.
Accordingly, a further embodiment of the present invention provides a compound the three-dimensional structure of which is simil-ar as a pharmacophore to the three-dimensional structure of the peptides of the present invention, the compound being characterized in that it reacts with antibodies raised against the peptides of the present invention and that the compound is capable of inhibiting TNF toxicity.
More detail regarding pharmacophores can lbe faund in Berlin et al., p. 150, Polinsky et al., p.,287, and Smith et al., p. 485,, in Smith and Rivier (eds.) "Peptides: Chemistry and Biology", Escom, Leiden {1991).
All of the molecules {proteins, peptides, ~~tc.) may be produced either by conventional chemical methods, as described herein, or by recombinant DNA methods.
The inventi.on.also provides DNA molecules encoding the.ligands according to the invention, vectors containing them and host cells comprising the vectors and capable of expressing the _ CA 02470670 2004-06-11 r ligands according to the invention.
The host cell may be either prokaryotic or eukaryotic. -The invention further provides DNA molecules hybridizing to the above DNA molecules and encoding ligands having the same activity.
The invention also provides pharmaceutical compositions coinprisirig the -above ligands which are useful for treating diseases induced. or Caused -by, the effects of TNF, either endogenously produced or exogenously administered.
BRIEF .~?ES~RI~T~C~N OF T~, ~G~RE~
Fic~t~,re 1 shows a diagrammatic illustration of the bacterial constructs used for determining the sequence to which antibodies of the 32 group bind.
Figure 2 shows an example of the Western blotting analysis technique by which the binding of the antibodies to the constructs shown in Figure 1 have been determined.
Figures ~ & 4 show the competition of synthetic peptides whose sequences contain the region of the epitope recognized by the monoclonal antibodies of the 32 group, or parts of it, with the binding of an antibody of this group to a construct comprising part of TBP-II in which this epitape is present. -io t r Figure 5 shows the nucleotide and deduced amino acid sequences of the p75 receptor. TBP-II and transmembrana:L domains are boxed and shaded. The region recognized by the group 32 antibodies is underlined.
Figure 6 shows the pattern of protection of HeLa p75.3 cells {as hereinafter defined) from TNF cytotcrxicity by different monoclonal antibodies against p75 TNF-R, and fragments thereof.
Figure 7 shows the effects of a monoclonal antibody against TBP-I
arid -several against TBP-II on the extent o:~ killing of U937 cells by TNF.
Figures 8a and 8b show the effects of monoclonal antibody 70 and Fab fragments thereof on the binding of T1~1F to HeLa p75.3 cells and U937 cells, respectively.
Figure 9 shows a comparison of the effects of the antibody 32 with other antibodies against the p75 TNF-R on TNF binding to HeLa p75.3 cells.
Figure 10 shows dissociation of TNF from HeLa p75.3 cells in the presence and absence of antibody no. 70 and its monovalent Fab fragment.
Figure 11 shows the nucleotide and deduced amino acid sequences for the CDR region of the heavy chain:> of three monoclonal antibodies of the 32 group.

Figure 12 shows the nucleotide and deduced amino acid sequences for the CDR region of the light chains of monoclonal antibody No.
32.
Figure 13 shows the sequence homology between several members of the TNF/NGF receptor family.
DETAILED D~SCR:~'BTION OF TF~E INVENTION
TNF, as stated above, is a cytokine which initiates its effect on cell ':function by binding to 'two specific cell ~ surface receptors the 'p55 arid p75 receptors. Binding of antibodies to the extracellular domain of these receptors can interfere with its effect. However, as shown in a number of studies, antibodies binding to the extracellular domain of the receptors can also txigger 'the.effects of TNF by inducing aggregation of the p55 receptors, as well as by inducing aggregation of the p75 receptors. (Engeimann, et al: J. Biol. Chem., 'Vo. 265, No. 24,.
pp. 14497-14504, 1990; and unpublished data) We have found that certain antibodies binding to one particular region in the p75 receptor are not mimetic but rather inhibitory to the signalling for the cytocidal effect by this receptor.
This, in spite of the fact that when binding .to this region, these antibodies do not block-TNF binding, but rather increase it to some extent.
The present invention reveals that this region recognized by r , these antibodies which we call the 32 group, is the region extending between the two C-terminal cysteines in the extracellular domain of the p75 receptor, plus an additional amino acid, thr179. This region fvr simplicity's sake, is called.
"cysteine loop" throughout this specification.
The present invention also provides the nucleotide sequences and deduced amino acid sequences in the CDR of the heavy chain of the three antibodies belonging to this group, r.~amed 32; 57 and 70. A
remarkable similarity between the sequence of amino acids in the CDR-of the heavy chain of the .32 and 70 antibodies was found, indicating that the sequence of amino acids in the CDR of the heavy chain ~of these two antibodies is. close to the optimum necessary for binding to the antigen. In addition, the invention also provides the nucleotide sequence and the deduced amino acid sequence of the light 'chain. of antibody 32. Based on these sequences, small molecular weight compounds, peptides or mimetic compounds which will inhibit the function of the p75 receptors cari be defined.
In evidence that such small compounds can indeed achieve this and that there is no need for aggregation of receptors, which antibodies are known to be able to do, it was found that also Flab) monovalent fragments of the antibodies of the 32 group inhibit signalling for toxicity by the p75 receptor when they are triggered. by TNF.
In view of these findings, as well as the close similarity of the receptors in this particular family, this-invention relates also to agents which bind to the C-terminal cysteine loop ~in the extracellular domain of the various other members of the TNF/NGF
receptor family and modulate the function of the other receptors, similarly to the modulation of the funcaion of TNF. In this receptor family, the localization of cysteine in the extra-cellular domain and the spacing is highly conserved. Certain members of this family, e.g. CDw40, exhibit particularly high similarity to the p75-receptor. Particularly in such receptors, agents binding to these xegions are expected to have effects similar to the effect of the 32 antibodies on the p75 receptor.
As stated above, the ligands according to the invention may comprise proteins, peptides, immunoadhesins, antibodies or other organic compounds.
Proteins may be isolated from cellular extracts, e.g, by ligand affinity purification employing a molecule having an amino acid sequence substantially corresponding to the above-mentioned stretch as ligand.
Peptides may be prepared by synthetizin.g first target peptides which correspond to the amino acid stretch of the TNF-R found in accordance with the invention to bind the ligands which inhibit the effects of TNF. Thereafter, peptide libraries axe screened for other ligands which bind thereto. The peptides which bind to these regions are further screened for those which also bind to TNF-R. Finally, the peptides capably of high affinity binding with both the target peptides and the TNF-R, are screened for the ability of the peptide to perform the desired biological activity.
In a similar manner, a variety of organic molecules, including drugs known for other indications, are screened for their ability to bind to the amino acid stretch found to be critical for inhibiting the effects of TNF.
In addition to the organic. molecules, also broth of biological matter such as~~bacteria culture. products, fungi~culture products, eukaryotic culture products and crude cytokirie preparations are screened with the amino acid target peptides described above.
Molecules obtained by this screening are then further screened for their ability to perform the desired bi.oiogical function.
Alternatively, molecules are designed which spatially fit the quaternary structure of the amino acid stretch in the receptor.
The active molecules obtained by the above procedures, inasfar as they are biological substances, can also be prepared by biotechnological approaches. In this way, massive production of these molecules will be made possible. Peptides may either be pxoduced by known peptide synthesis methods or using expression vectors containing DNA sequences encoding them. Other molecules, if produced in an enzymatic way, can be made by producing the enzymes involved in the appropriate cultured cells.
15.

Pharmaceutical compositions containing the .l.igands of the present invention may be employed fox antagonizing the effects of TNF in mammals.
Such compositions comprise the ligands according to the invention.
as their active ingredient. The pharmaceutical compositions are indicated for conditions such as septic shock, cachexia, graft-versus-host reactions, autoimmune diseases such as rheumatoid arthritis, and the like. They are also indicated for counter-acting e.g. an overdose of exogenously administered TNF.
The pharmaceutical compositions according to the invention are administered depending on the condition to be treated, via the accepted ways.of administration. For example, in the case of septic shock, intravenous administration will be preferred. The pharmaceutical compositions may also be administered continuously, i.e. by way of infusion, or orally. The formulation and dose will depend on.the condition to be treated, the route of administration and the condition and th.e body weight of the patient to be txeated. The exact dose will be determined by the attending physician.
The pharmaceutical compositions according .to the invention are prepared in the usual manner, for example by mixing the active ingredient with pharmaceutically and physiologically acceptable carriers and/or stabi3izers and/or excipients, as the case may be, and are prepared in dosage form, e.g. by iyophilization in dosage vials.

f a As used herein the teran "muteins" refers to analogs of the proteins, peptides and the like in which.one or more of the amino acid residues of the protein found to bind are replaced by different amino acid residues or are deleted, or one or more amino acid residues are added to the original sequence, without changing considerably the activity of tl~e resulting product.
These muteins are prepared by known synthesis and/or by site-directed mutagenesis techniques, or any other known technique suitable therefor.
The term "fused protein" refers to a poly;?eptide comprising the ligands or a mutein thereof fused with another protein which has an extended residence time in body fluids" The ligands may thus be fused to another protein, polypeptide or the like, e.g. an immunoglobulin or a fragment thereof.
The term "salts" herein refers to both sa:Lts of carboxyl groups and to acid addition salts of .amino graups of the ligands, muteins and fused proteins thereof. Salts of a carboxyl group may be formed by means known in the art and include inorganic salts, for example, sodium, calcium, ammonium, ferric or zinc salts, and the like, and salts with organic bases as those formed, for example, with amines, such as, triethanalamine, arginine or lysine, piperidine, procaine and the like. Acid addition salts include, for example, salts with minera:L acids such as, for example, hydrochloric acid or sulfuric acid, and salts with organic acids such as, for example, acetic acid or oxalic acid.

"Functional derivatives'° as used herein cover derivatives of the ligands and their fused proteins and muteins, which may be prepared from the functional groups which occur as side chains on the residues or the N- or C- terminal groups, by means known in the art, and are included in the invention as long as they remain pharmaceutically acceptable, i.e. they do not destroy the activity of the ligand and do not confer toxic properties vn compositions containing it. These derivatives may, for example, include polyethylene glycol side-chains wh_Lch may mask antigenic sites and extend theresidence of the ligands in body fluids.
(7ther derivatives include aliphatic esters of the carboxyl groups, amides of the carboxyl groups by reaction with ammonia or with primary or secondary amines, N-acyl derivatives of free amino groups of the amino acid residues formed with acyl moieties (e. go alkanoyl or carbocyclic aroyl groups) or O-acyl derivatives of free hydroxyl groups (for example that of Beryl or threonyl residues) formed with aryl moieties.
The invention is illustrated by the following non-limiting examples:
EXAMPLE l: Determinat,~on of the_ region of 'the p75 receptor which is recocrnized by the group ~2 antibodies In example 5 of the main application, a number of constructs were prepared by expression in E. coli, and it was concluded that the epitope recognized by antibody no. 32 maps between amino acids 125-182.

We have now prepared further constructs and the complete list of constructs examined, as well as their relationship to the ;
structure of the soluble p75R are shown in Fig. 1. Constructs recognized by the antibodies of the' 32 group are listed in bold numbers and illustrated as solid lines. Those not reacting. with these antibodies are listed in thin numbers and illustrated by broken lines. All constructs are identified by their N- and C-terminal amino acid residues.
Figure 1_, above the diagrammatic illustration of the constructs, show, the amino acid sequence of part of the p75 TNF-R, the regions corresponding to the soluble form oaf the receptor and the transmembranal region being boxed. Amino acid residues conserved between man and mouse are underlined.
The Western blotting analysis. shown in. Figure 2, of the binding of the groug 32 antibodies to some of the constructs shown in Figure 1 was carried out as in Example 5 of the main application..
EXPZE 2L Competition fc~ bindina to the e~xtracellular domain of th 7 T -R etwee ro a tib di s a d s t eti a id s A number of synthetic peptides whose sequences correspond to various parts of the region on the TNF-R suspected to be the group 32 epitope were synthetized (residues 160-179, 162-179, 163-179, 165-179 and 157-179). The peptid~a were examined in an ELISA test for their ability to compete for the binding to the antibodies of the 32 group.
i i A bacterially produced construct corresponding to amino acids 3 to 180 of the p75 TNF-R (p75 construct in P'ig. 3) was applied, at the indicated concentrations, to PVC plates precoated with antibody 32 followed by application of rabbit antiserum to TBP-II
(p75 soluble TNF-R). The amount of rabbit antiserum bound to the plate was determined by applying goat antiserum against rabbit immunoglobulin, coupled to horse-radish peroxidase and enzymatic assessment of the amount of goat immunoglobulin bound to the plate. Figure 3 shows the data of an experiment in which a synthetic peptide corresporiding~to amino acid residues 163 to 179 was found to compete for the binding.
Figure 4 shows the data of an eXperiment in which a fusion protein of maltose binding protein (MBP) with the sequence of amino acids extending from 125 to 192 of the p75 receptor was used to coat PVC plates at a concentration of 10~,g/mi, then the No. 32 McAb was applied at a concentration of 2~,g/ml 'together with the indicated concentrations of different peptides:
DW16 - amino acids 165-179 DW18 -armno acids 163-179 DW19 - amino acids 162-279 DW21 - amino acids 160-179 Thereafter, the reaction was developed by .adding goat anti-mouse coupled to horseradish peroxidase. As shown in Fig. 4, marked ~.nhibition of fusion protein recognition by monoclonal antibody No. 32 was observed only with the three peptides covering the whole epitope.

EXAMPLE ~: Mutational'study of the 32 epitone Replacing cysteine 178 with alanine in a recombinant peptide whose sequence corresponds to amino acids 3 to 181, made this protein unrecognizable by the 32 group antibodies. This finding suggests that in order to be recognized by these antibodies, the two cysteines in the group 32 epitope region must be free to interact with each other; i.e. that the structure recognized by the antibodies is a loop. In. support of this notion, we found that reduction of the peptide with dithiothreitho7: prior to SDS
PAGE and Western blotting analysis somewhat decreased the effectivity of its recognition by the 32 group antibodies, and reduction by dithiothreithol followed by alkylation with iodoacetimide made it completely unrecognizable by the antibodies.
EXAMPLE 4: Effects of various ,~ntiboc.~'te~ and fragments thereof on TNF toxicity {a) In order to compaxe the~funetion of the 32 group antibodies, not only to.antibodies which bind to the.receptor upstream to the 32 epitope region (as must of the anti-TBP-II antibodies are expected to), but also to antibodies that bind to the receptor downstream to that epitope region, we immunized mice with a chimeric construct corresponding to the region extending downstream to the 32 epitope {amino acids 181 to 235; the "stalk" region), linked to MBP. The rabbits developed antibodies which bound to the chimera with which they were immunized as well as to the intact p55 TNF
receptor.. These antibodies were affinity purified by binding *
to the chimeric protein, linked to an Affi-gel 10 column, and tested for effect on TNF function and binding: {The affinity purified antibody preparation was termed "318").
(b) All monoclonal anti-TBP-II antibodies as well as the affinity .
purified antistalk antibodies were tested for effect on TNF
toxicity in clones of, the epitheloid l3eLa cells which were made to over-express the p75-receptors ~(by their transfection Swith the p75 receptor's cDNA. We called the particular over expressing clone used in the experiments presented here, HeLa p75.3). The only antibodies found to inhibit TNF function were the antibodies of the group 32 epitope; that; in spite of the fact that they do not inhibit, but somewhat increase TNF.binding to the receptor (Figs. S and 9). Two of the other anti-TBP-II antibodies (No. 67 of Figs. 6 and 9 and number 81) had very little effect on TNF binding to the receptor or.
on TNF toxicity. All other monoclonal anti-TBP-II antibad~.es somewhat potentiated the cytocidal effect of TNF .even though competing with TNF binding {e.g. antibody 36 of Figs 6 and 9). The "anti-stalk" antibodies had very little effect on TNF
binding or function {Figs. 5 and 9). Applying the anti-stalk antibodies on the cells together with antibodies of the 32 group did not interfere with the inhibitory effect of the latter on TNF function.
(c} The same ganel of antibodies was tested for effect on the *Trade-mark killing of the myelocytic U937 cells by TNF. As opposed to the mimetic effect of anti-TNF receptor antibodies in the IieLa cells, neither anti-p55 nor anti-p75 receptor antibodies were found to be mimetic to the cytocidal effect of TNF on the U937 cells under the. conditions of the experiment carried out. Having no ability to mimic the effect of TNF, all monoclonal antibodies which evmpete for TNF binding either to the p75 receptor, {e.g. antibodies 14, 31 and 36 of Figure 9) or to the p55 receptor (e.g. antibody number 18 of Figure 7) are inhibitory to the TNF effects. Antibodies which had no effect on TNF binding to the receptors {e.g. number 67 of Figure 9) had no effect on TNF function (Figure 6). The 32 group antibodies were unique in having an ability to inhibit TNF function in this cell without having any inhibitory effect on TNF binding. The antibodies actually enhanced the binding of TNF to these, cells, much more so than in the HeLa p75:3 cells (Figure 8). The inhibitory effect of the 32 group antibodies was additive to that of antilbodies which block TNF
binding to the p55 receptor (e.g. the combination 18/32 in Figure . 7 ) .
F~ArmLE 5 ~ Effect of crroup, ~~2 antibodies and Fab monav 1~ ant fracrments thereof on t~c~,issociati~n of TNF from the T~tF-Rs In order to explore the .mechanism by which the 32 group antibodies cause an indrease in TNF binding, we compared the xate of TNF dissociation from HeLa p75.3 tails in the presence and absence of these antibodies.

Radiolabelled TNF was added to confluent HeLa p75.3 cells and the cells were incubated for 2 hr on ice . Unbound ligand was washed away and l ml of binding buffer containg 500 ng/ml of cold TNF
was applied into quadruplicate wells for the indicated periods of time on ice. Thereafter, the wells were washed once again with cold PBS, and amount of residual li.gand was determined by measuring radioactivity of cells detached from. plates by incubation with PBS/EDTA solution. The antibodies were applied throughout the assay at a concentration of l.Uwg/ml.
As illustrated in Fig. 10, both these antibodies as well as their F(ab) monovalent fragments caused a decrease in the rate of TNF
dissociation from the receptors. Besides providing a possible explanation for the way in which these antibodies affect TNF
binding to its receptors, this finding indicated an additional application for this effect. Salable forms of the p75 TNT-Rs or of the p55 receptor or of any other member of the TNF/BGF.
' receptor fami.iy in which a conformational change as that imposed by the 32 group antibody will occur, will serve as better inhibitors of the respective agonist.
E L 6: Dete t'o o nuc eo i a se ces an de uc d amino acid seciuences in the CDR of the heavy chains of monoclor.~
antibodies32. 57 and~Q~~rou~?3? anti died and in the CDR of the light tKappa~ chain of antibody 32 In order to determine the nucleotide sequences of the CDR of the heavy chains of antibodies 32, 57 and 70, total RNA was isolated by the Promega protocol from the respective h.ybridoma cells, with the use of guanidinium thioisocyanate. First strand cDNA
synthesis on this RNA was performed with the use of AMV reverse transcriptase and eithex~oligo(dT)1.5-18 or an oligonucleotide complementary to the constant region of the heavy chain of murine IgG as a primer. The cDNA was used as a template for PCR, applying a partially degenerate 5'-Primer. 40 cycles of PCR were carried out. PCR products with the size of about 350 by were purified electrophoretical:ly and, cloned into the. Bluescript*
vector. Clones having inserts of the right size were sequenced.
Double-stranded cDNA .of the CDR region of'the light chain of antibody no. 32 was synthesized in a similar ~c~ranner.
The nucleotide sequences obtained by i~he dideoxy chain termination method, and the amino acid sequences deduced therefrom are shown in Figures 11 and 12. The CDR1, 2 and 3 regions are underlined. w EXAMPLE 7: Preparationof scFv of_~he 3~c~rouo antibodies The cloned variable regions of the heavy and light chains of the monoclonal antibodies of the 32 group are linked with a linker of 15 amino acid length and introduced into a commercial expression vector. The vector contains a promoter, e.g. lac, a leader sequence e.g. pel-B, as well as a sequence encoding a small peptide ("tag" peptide) against crhich a .monoclonal antibody is commercially available. The plasmid is nowintroduced into E.
coli and the bacteria are grown to O.D: 0.5-1Ø Expression of scFv is induced by addition of IPTG and growth is continued for *Trade-mark another 6-24 hrs. The soluble scFv-tag complex is then isolated from the culture medium by immunoaffinity purification using the monoclonal antibody against the tag and then purified on a metaloaffinity column.
Any scFv accumulating within the bacteria is purified by isolating and repeatedly washing the inclusion bodies, followed by solublization by e.g. urea or guanidinium and subsequent renaturation.
Alternative possibilities are employing an oligohistidine as the tag, using a stronger promoter instead of lac, i.e. T7, constructing the vector without the leader sequence or introducing a sequence encoding a "tail" of irrelevant sequences into the vector at the 5' end of the scFv. This "tail" should not be biologically active, since its only purpose is the creation of a longer molecule than the native scFv, thus causing a longer retention time in the body.
EXA~~E 8: .
Figure 13 shows the internal cysteine rich repeats in the extracellular domains of the two TNF-Rs and their alignment with the homologous repeats in the extracellular domain of the human FAS, nerve growth factor receptor (NGF) anal CDw40, as well as rat Ox40. The amino acid sequences (one letter symbols) are aligned for maximal homology. The positions of the amino acids within the receptors are denoted in the left hand margin.

E 9s r at'on f ec 'n nt D A of a es co r's' a ot'de s a es od' or t a dive a t'de d th nnolecules and their expression The peptides and other molecules can also be prepared by genetic engineering techniques and their preparation encompasses all the tools used in these techniques. Thus DNA mo7:ecules are provided which comprise the nucleotide sequence coding for such peptides and other biological molecules. These DNA molecules can be genomic DNA, cDNA, synthetic DNA and a combination thereof.
Creation of DNA molecules coding for such peptides and molecules is carried out by conventional means, once the amino acid sequence of these peptides and other molecules has been determined.
Expression of the recombinant proteins can be effected in eukaryotic cells, bacteria or yeasts, using the appropriate expression vectors. Any method known .in the art may be.
employed.
For example, the DNA molecules coding for the peptides or other molecules obtained by the above methods are inserted into.
appropriately constructed expression vectors by techniques well known in the art (see Maniatis, T. et al., Molecular Cloninq: A
Laboratory Manual, Cold .Spring Harbor Labozatory, Cold Spring Harbor (1982)). Double-.stranded cDNA is 3.i.nked to plasmid vectors by homopalymeric tailing or by restriction .inking involving the use of synthetic DNA linkers or blunt-ended ligation techniques.

DNA ligases are used to ligate the DNA molecules and undesirable joining .is avoided by treatment with alkaline phosphatase.
In order to be capable of expressing a desired biological substance, i.e. a peptide or protein (hereinafter "protein~', for simplicity's sake), an expression vector should comprise also specific nucleotide sequences containing transcriptional and translational regulatory information linked to the DNA coding for the desired protein in such a way as to permit gene expression and production of the protein. First, in order for the gene to be transcribed, it must be ;preceded by a promoter recognizable by RNA polymerase, to Which the polymerase binds and thus initiates the transcription process. There are a variety of such promoters in use, which work with different efficiencies (strong and weak promoters). They are different for prokaryotic and eukaryotic cells.
The promoters that can be used in the present invention may be, ', either constitutive, for example, the i.nt promoter of.
bacteriophage lambda, the bla promoter of i:he ~-lactamase gene of pBR322, and the GAT promoter of the chloramphenicol acetyl transferase gene of pPR325, etc., or iiaducible, such as the prokaryotic promoters including the major right and left promoters of bacteriophage lambda (P~ and PA), the trp, ~ecA, lacZ, cI, ompF_ and c,~,al promoters of E;, coli, or the trp,-lac hybrid promoter, etc. (click, B.R. (1,3$7) J.Ind.Microbiol., 1_:277-282).

Besides the use of strong promoters to generate large quantities of mRNA, in order to achieve high levels of gene expression in prokaryotic cells, it is necessary to use also ribosome-binding sites to ensure that the mRNA is efficiently translated. One example is the Shine-Dalgarno (SD) sequence appropriately positioned from the initiation codon and complementary to the 3'-terminal sequence of 16S RNA.
Far eukaryotic hosts, different transcriptional and translati.onal regulatory sequences may be employed, depending on the nature of the host. They may be derived from viral sources,, such as adenovirus, bovine papilloma virus, Simian virus, or the like, where the regulatory signals are associated with a particular gene which has a high level of expression. Examples are the TIt promoter of Herpes virus, the SV40 early promoter, the yeast gal4 gene promoter, etc. Transcriptional initiation regulatory signals may be selected which allow for repression and activation, so that expression of the genes can be modulated.
The DNA molecule comprising the nucleotide sequence coding for the peptides or other molecules of the invention and the operably linked transcriptional and translational regulatory signals is inserted into a vector Which is capable of integrating the desired gene sequences into the host cell chromosome. The cells which have stably integrated the introduced DNA into their chromosomes can be selected by also Lntroducing one or more markers which allow for selection of host cells which contain the expression vector. The marker may provide for prototrophy to an a auxotropic host, biocide resistance, e.g., antibiotics, or heavy metals, such as copper, or the like. The selectable marker gene can either be directly linked to the DNA gene sequences to be expressed, or introduced into the same cell by co-transfecti.on.
Additional elements may also be needed for optimal synthesis of single chain binding protein mRNA. These elements may include splice signals, as well as transcription promoters, enhancers, and termination signals. cDNA expression vectors incorporating such elements include those described by Ok:ayama, H., (1983) Mol.
Cell Biol., 3:280.
In a preferred embodiment, the introduced DNA molecule will be incorporated into a plasmid or viral vector capable of autonomous replication in the recipient. host. Factors of importance in selecting a particular plasmid or viral vector include: the ease with which recipient cells that contain the vector may be recognized and selected from those recipient cells Which' do not contain the vector; the number of copies of the vector which are desired in a particular host; and whether it is desirable to be able to "shuttle" the vector between host cells of different species.
Preferred prokaryotic vectors include plasmids such as those capable of replication in E. coli, for example, pBR322, ColEl, pSC301, pACyC 184, etc. (see Maniatis et al., (1982) op. cit.);
Bacillus plasmids such as pC194, pC221, pT1''?, etc. (Gryczan, T., the Molecular Bioloav of the Bacilli., Academic Press, NY (1982j);
Streptomyces plasmids including pIJ101 (K~endall, R.J. et al., Fk (1987) J.Bacteriol. ,~G~:4177-83},; Streptomyces bacteriophages such as ~C31 (Chater,- R.F. et al., in: Sixth I~t~,~nat~nal ;~rmnosium on Actinomycetales l~ioloav, {1986)), and Pseudomonas plasmids (John, J.F., et al. (1986} Rev. Infect.Dis. $:693-704;
and Izaki, R. (1978} Jpn. J. Bacteriol., x:729-742}.
Preferred eukaryotic plasmids include BPV, vaccinia, SV40, 2-micron circle, etc., or their derivatives. Such plasmids are well known in the art (Botstei.n, D., et a:L. (1982) Miami Wint.
Symp..,l~, pp. 265-274; Broach, J.R., in: T a Molecular Biology of the Yeast Saccharomxces~ Life Cycle and Inheritance, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp. 445-470 (1981);
Broach, J.R., (1982} Cell, x$:203-204; Hollon, D.P., et al.
(1980) J. Clin. Hematol. Oncol., ,0:39-48; Maniatis, T., in:_Cell ~oloqy~ A Co~ngrehensive treatise. Vol. 3: Gene Expression, Academic Press, NY, pp. 563-608 {1980)).
Once the vector or DNA sequence containing the constructs) has been prepared for. : expression, the DNA construct { s } may be introduced into an appropriate host cell by any of a variety of suitable means: transformation, transiection, conjugation, protoplast fusion, electroporation, calcium phosphate-p~ecipitation, direct microinjection, etc.
Host cells to be used in this invention may be either prokaryotic or eukaryotic. Preferred prokaryotic hosts include bacteria such as E. cgli, Bacillus, Streptomyces, Pseudomonas, Salmonella, Serratia, etc. The most preferred prokaryotic host is E. coli.

w Bacterial hosts of particular interest include E. coli K12 strain 294 (ATCC 31446), E~ o~li X1776 (ATCC 31537), E. coli W3110 {F', lambda , prototropic {ATCC 27325)), and other enterobacterium such as Salmonella typhimurium or Serratia marcescens and various Pseudomonas sgecies. Under such conditions; the protein will not be glycosylated. The prokaryotic host must. be compata.ble with the replicon and -control sequences in the expression plasmid.
Preferred eukaryotic hosts are mammalian cells; e.g. human, monkey, mouse and Chinese hamster ovary (CT30) cells, because they provide post-translational modifications to protein molecules including correct folding or glycosylation at correct sites. Also yeast cells can carry out post-translational peptide modifications including giycosylation. A number of recombinant DNA strategies exist which utilize strong gromoter sequences and high copy number of plasmids which can be utilized for production of the desired proteins in yeast. Yeast recognizes leader sequences on cloned mammalian gene products and secretes peptides bearing leader sequences (i.e. pre-peptides).
After.the.introduction of the vector, the host cells are grown in a selective medium, which selects for the growth of veetor-containing cells. Expression of the cloned gene sequences) results in the production of the desired proteins.
Purification of the recombinant proteins is carried.out by any one of the methods known for this purpose.

Deposit Information Hybridoma TBP-II 70-2 was deposited with the Collection National de Cultures de Microorganismes; Institut Pasteur (CNCM? on March 12,1990 and assigned No. I-928.
Hybridoma TBP-11. 32-5 was deposited with the CNCM on 1 Sept. 1993 and assigned No. I-1358.

Claims (6)

1. A method of producing a soluble p50 TNF-R or p75 TNF-R or a member of the TNF/NGF receptor family with a decreased rate of the dissociation of TNF or the respective natural ligand from the receptor comprising introducing a mutation into the C-terminal cysteine loop of such receptor, wherein the cysteine loop includes the amino acid sequence Cys-163 to Thr-179 in the p75 TNF-R, or a corresponding region in another member of the TNF/NGF receptor family and wherein said mutation imposes a conformational change in said receptor resulting in a decreased dissociation rate of TNF
or the respective natural ligand from the receptor.

2. A soluble p50 TNF-R or p75 TNF-R or a member of the TNF/NGF
receptor family with a decreased rate of the dissociation of TNF
or the respective natural ligand from the receptor obtainable according to the method of claim 1.

3. A pharmaceutical composition comprising the soluble receptor according to claim 2 and a pharmaceutically acceptable diluent or carrier.

4. The use of a receptor according to claim 2 in the preparation of a pharmaceutical composition for antagonizing the effects of TNF.

5. The use of a receptor according to claim 2 in the preparation of a pharmaceutical composition for the treatment of septic shock, cachexia, graft-versus-host diseases or autoimmune diseases.

6. The use of a receptor according to claim 2 in the preparation of a pharmaceutical composition for the treatment of rheumatoid arthritis.