CA2455686A1 - Procedes de production de microbes resistant aux antibiotiques et nouveaux antibiotiques - Google Patents

Procedes de production de microbes resistant aux antibiotiques et nouveaux antibiotiques Download PDF

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Publication number
CA2455686A1
CA2455686A1 CA002455686A CA2455686A CA2455686A1 CA 2455686 A1 CA2455686 A1 CA 2455686A1 CA 002455686 A CA002455686 A CA 002455686A CA 2455686 A CA2455686 A CA 2455686A CA 2455686 A1 CA2455686 A1 CA 2455686A1
Authority
CA
Canada
Prior art keywords
leu
ser
glu
lys
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002455686A
Other languages
English (en)
Inventor
Nicholas C. Nicolaides
Philip M. Sass
Luigi Grasso
J. Bradford Kline
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Morphotek Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2455686A1 publication Critical patent/CA2455686A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C12Q1/20—Testing for antimicrobial activity of a material using multifield media

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés de production de bactéries résistant aux antibiotiques, qui comporte les étapes consistant à : bloquer dans une bactérie la réparation de mésappariements afin d'obtenir des bactéries hypermutables ; mettre en contact les bactéries avec au moins un antibiotique ; sélectionner les bactéries résistant à l'antibiotique ; et mettre en culture les bactéries résistant à l'antibiotique. L'invention concerne aussi des procédés visant à déterminer les gènes responsables de la résistance aux antibiotiques.
CA002455686A 2001-07-25 2001-07-25 Procedes de production de microbes resistant aux antibiotiques et nouveaux antibiotiques Abandoned CA2455686A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2001/023888 WO2003012130A1 (fr) 2001-07-25 2001-07-25 Procedes de production de microbes resistant aux antibiotiques et nouveaux antibiotiques

Publications (1)

Publication Number Publication Date
CA2455686A1 true CA2455686A1 (fr) 2003-02-13

Family

ID=21742739

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002455686A Abandoned CA2455686A1 (fr) 2001-07-25 2001-07-25 Procedes de production de microbes resistant aux antibiotiques et nouveaux antibiotiques

Country Status (3)

Country Link
EP (1) EP1417329A4 (fr)
CA (1) CA2455686A1 (fr)
WO (1) WO2003012130A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60329526D1 (de) 2002-11-15 2009-11-12 Morphotek Inc Verfahren zur erzeugung einer hohen antikörperproduktion von durch in-vitro-immunisierung entstandenen hybridomen
TWI778025B (zh) * 2017-02-24 2022-09-21 碁米庫斯生物科技股份有限公司 抗生素檢測及篩選系統

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY103919A (en) * 1988-03-30 1993-10-30 Kao Corp Mutant resistant to cell membrane synthesis inhibitor and process for preparing the same.
DE19717346C2 (de) * 1997-04-24 1999-05-20 Max Planck Gesellschaft DNA-Sonden, Verfahren und Kit zur Identifizierung Antibiotika-resistenter Bakterienstämme
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
JP5036119B2 (ja) * 2001-01-15 2012-09-26 モーフオテク・インコーポレーテツド ミスマッチ修復の化学阻害剤

Also Published As

Publication number Publication date
WO2003012130A1 (fr) 2003-02-13
EP1417329A1 (fr) 2004-05-12
EP1417329A4 (fr) 2005-05-11

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued