CA2453852C - Spectrometre de masse - Google Patents
Spectrometre de masse Download PDFInfo
- Publication number
- CA2453852C CA2453852C CA2453852A CA2453852A CA2453852C CA 2453852 C CA2453852 C CA 2453852C CA 2453852 A CA2453852 A CA 2453852A CA 2453852 A CA2453852 A CA 2453852A CA 2453852 C CA2453852 C CA 2453852C
- Authority
- CA
- Canada
- Prior art keywords
- ions
- ion mobility
- time
- mass
- flight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 150000002500 ions Chemical class 0.000 claims abstract description 1259
- 230000003595 spectral effect Effects 0.000 claims abstract description 123
- 238000001819 mass spectrum Methods 0.000 claims abstract description 51
- 230000037230 mobility Effects 0.000 claims description 552
- 238000005040 ion trap Methods 0.000 claims description 76
- 238000000034 method Methods 0.000 claims description 58
- 238000012545 processing Methods 0.000 claims description 54
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- 238000004458 analytical method Methods 0.000 claims description 26
- 238000004949 mass spectrometry Methods 0.000 claims description 19
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- 230000007423 decrease Effects 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000010265 fast atom bombardment Methods 0.000 claims description 4
- 239000012634 fragment Substances 0.000 claims description 4
- 238000009616 inductively coupled plasma Methods 0.000 claims description 4
- 238000004992 fast atom bombardment mass spectroscopy Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000005036 potential barrier Methods 0.000 claims description 3
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- DZHSAHHDTRWUTF-SIQRNXPUSA-N amyloid-beta polypeptide 42 Chemical compound C([C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](C(C)C)C(=O)NCC(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(O)=O)[C@@H](C)CC)C(C)C)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC=1C=CC=CC=1)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(O)=O)C(C)C)C(C)C)C1=CC=CC=C1 DZHSAHHDTRWUTF-SIQRNXPUSA-N 0.000 claims description 2
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 claims description 2
- 238000000451 chemical ionisation Methods 0.000 claims description 2
- 238000003795 desorption Methods 0.000 claims description 2
- 108010026389 Gramicidin Proteins 0.000 description 17
- -1 Leucine Enkephalin ions Chemical class 0.000 description 17
- 229950009774 gramicidin s Drugs 0.000 description 17
- 238000000926 separation method Methods 0.000 description 14
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- 108010022337 Leucine Enkephalin Proteins 0.000 description 11
- 108090000623 proteins and genes Proteins 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 10
- IUAYMJGZBVDSGL-XNNAEKOYSA-N gramicidin S Chemical compound C([C@@H]1C(=O)N2CCC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@H](C(N[C@H](CC=2C=CC=CC=2)C(=O)N2CCC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CCCN)C(=O)N[C@@H](CC(C)C)C(=O)N1)C(C)C)=O)CC(C)C)C(C)C)C1=CC=CC=C1 IUAYMJGZBVDSGL-XNNAEKOYSA-N 0.000 description 9
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- 238000001914 filtration Methods 0.000 description 4
- URLZCHNOLZSCCA-UHFFFAOYSA-N leu-enkephalin Chemical compound C=1C=C(O)C=CC=1CC(N)C(=O)NCC(=O)NCC(=O)NC(C(=O)NC(CC(C)C)C(O)=O)CC1=CC=CC=C1 URLZCHNOLZSCCA-UHFFFAOYSA-N 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000004885 tandem mass spectrometry Methods 0.000 description 4
- 108010092674 Enkephalins Proteins 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 238000010408 sweeping Methods 0.000 description 3
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 101800001442 Peptide pr Proteins 0.000 description 2
- 239000012491 analyte Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
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- 238000001514 detection method Methods 0.000 description 2
- 238000000132 electrospray ionisation Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000010884 ion-beam technique Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000000737 periodic effect Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 1
- 241000937413 Axia Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 101100346656 Drosophila melanogaster strat gene Proteins 0.000 description 1
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 1
- URLZCHNOLZSCCA-VABKMULXSA-N Leu-enkephalin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)CNC(=O)CNC(=O)[C@@H](N)CC=1C=CC(O)=CC=1)C1=CC=CC=C1 URLZCHNOLZSCCA-VABKMULXSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 239000003990 capacitor Substances 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 230000005405 multipole Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- MLGCXEBRWGEOQX-UHFFFAOYSA-N tetradifon Chemical compound C1=CC(Cl)=CC=C1S(=O)(=O)C1=CC(Cl)=C(Cl)C=C1Cl MLGCXEBRWGEOQX-UHFFFAOYSA-N 0.000 description 1
- VLPFTAMPNXLGLX-UHFFFAOYSA-N trioctanoin Chemical compound CCCCCCCC(=O)OCC(OC(=O)CCCCCCC)COC(=O)CCCCCCC VLPFTAMPNXLGLX-UHFFFAOYSA-N 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/26—Mass spectrometers or separator tubes
- H01J49/34—Dynamic spectrometers
- H01J49/40—Time-of-flight spectrometers
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0036—Step by step routines describing the handling of the data generated during a measurement
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
La présente divulgation porte sur un spectromètre de masse dans lequel des ions traversent un séparateur à mobilité ionique (1) puis subissent une analyse de masse dans un analyseur de masse à temps de vol (2). De multiples jeux de données spectrales de masse sont obtenus, ensuite posttraités, de sorte que les données spectrales de masse relatives aux ions présentant un ou des états de charge non désirés sont éliminées. Le spectre de masse résultant comprend des ions présentant l'état de charge voulu.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0313054.9 | 2003-06-06 | ||
| GBGB0313054.9A GB0313054D0 (en) | 2003-06-06 | 2003-06-06 | Mass spectrometer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2453852A1 CA2453852A1 (fr) | 2004-12-06 |
| CA2453852C true CA2453852C (fr) | 2012-07-10 |
Family
ID=9959471
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2453852A Expired - Lifetime CA2453852C (fr) | 2003-06-06 | 2003-12-22 | Spectrometre de masse |
Country Status (3)
| Country | Link |
|---|---|
| CA (1) | CA2453852C (fr) |
| DE (2) | DE10361023B4 (fr) |
| GB (2) | GB0313054D0 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0420408D0 (en) | 2004-09-14 | 2004-10-20 | Micromass Ltd | Mass spectrometer |
| GB0522933D0 (en) * | 2005-11-10 | 2005-12-21 | Micromass Ltd | Mass spectrometer |
| GB0624993D0 (en) * | 2006-12-14 | 2007-01-24 | Micromass Ltd | Mass spectrometer |
| DE102008051695B4 (de) | 2008-09-04 | 2019-06-06 | Bruker Daltonik Gmbh | Ionenmobilitätsmessung an Potentialbarriere |
| GB201304039D0 (en) * | 2013-03-06 | 2013-04-17 | Micromass Ltd | Time shift improved IMS digitisation |
| WO2014135865A1 (fr) | 2013-03-06 | 2014-09-12 | Micromass Uk Limited | Décalage temporel pour spectrométrie de mobilité ionique ou numérisation de séparation améliorées |
| GB201402584D0 (en) * | 2014-02-14 | 2014-04-02 | Micromass Ltd | Flushing IMS cell between IMS cycles |
| US10352900B2 (en) | 2014-02-14 | 2019-07-16 | Micromass Uk Limited | Flushing ion mobility separation cell between ion mobility separation cycles |
| GB201407611D0 (en) * | 2014-04-30 | 2014-06-11 | Micromass Ltd | Mass spectrometer with reduced potential drop |
| WO2015166251A1 (fr) | 2014-04-30 | 2015-11-05 | Micromass Uk Limited | Spectromètre de masse à chute de potentiel réduite |
| GB201617710D0 (en) * | 2016-10-19 | 2016-11-30 | Micromass Uk Limited | Ion separator |
| GB201621927D0 (en) * | 2016-12-22 | 2017-02-08 | Micromass Ltd | Mass spectrometric analysis of lipids |
| GB2575168B (en) * | 2018-06-04 | 2022-06-01 | Bruker Daltonics Gmbh & Co Kg | Precursor selection for data-dependent tandem mass spectrometry |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2205713A1 (de) * | 1972-02-08 | 1973-08-23 | Viktor Dr Ing Winkler | Doppel-ionenstrahlverfahren fuer quantitative (absolute) massenspektrometrische messungen |
| US4823368A (en) * | 1987-06-30 | 1989-04-18 | Rikagaku Kenkyujyo | Open counter for low energy electron detection with suppressed background noise |
| US5073713A (en) * | 1990-05-29 | 1991-12-17 | Battelle Memorial Institute | Detection method for dissociation of multiple-charged ions |
| CA2391060C (fr) * | 2001-06-21 | 2011-08-09 | Micromass Limited | Spectrometre de masse |
| US7586088B2 (en) * | 2001-06-21 | 2009-09-08 | Micromass Uk Limited | Mass spectrometer and method of mass spectrometry |
| CA2391140C (fr) * | 2001-06-25 | 2008-10-07 | Micromass Limited | Spectrometre de masse |
| GB2389704B (en) * | 2002-05-17 | 2004-06-02 | * Micromass Limited | Mass Spectrometer |
| EP1378930B1 (fr) * | 2002-05-30 | 2009-04-15 | Micromass UK Limited | Spectromètre de masse |
| GB2392304B (en) * | 2002-06-27 | 2004-12-15 | Micromass Ltd | Mass spectrometer |
-
2003
- 2003-06-06 GB GBGB0313054.9A patent/GB0313054D0/en not_active Ceased
- 2003-12-22 CA CA2453852A patent/CA2453852C/fr not_active Expired - Lifetime
- 2003-12-23 GB GB0329883A patent/GB2409764B/en not_active Expired - Lifetime
- 2003-12-23 DE DE10361023A patent/DE10361023B4/de not_active Expired - Lifetime
- 2003-12-23 DE DE20319990U patent/DE20319990U1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE10361023A1 (de) | 2005-01-13 |
| CA2453852A1 (fr) | 2004-12-06 |
| DE10361023B4 (de) | 2010-04-08 |
| DE20319990U1 (de) | 2004-05-19 |
| GB2409764B (en) | 2006-05-24 |
| GB0329883D0 (en) | 2004-01-28 |
| GB2409764A (en) | 2005-07-06 |
| GB0313054D0 (en) | 2003-07-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20231222 |