CA2445884A1 - Elucidation de la fonction de genes - Google Patents

Elucidation de la fonction de genes Download PDF

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Publication number
CA2445884A1
CA2445884A1 CA002445884A CA2445884A CA2445884A1 CA 2445884 A1 CA2445884 A1 CA 2445884A1 CA 002445884 A CA002445884 A CA 002445884A CA 2445884 A CA2445884 A CA 2445884A CA 2445884 A1 CA2445884 A1 CA 2445884A1
Authority
CA
Canada
Prior art keywords
array
cells
nucleic acids
library
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002445884A
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English (en)
Inventor
Benjamin J. Doranz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2445884A1 publication Critical patent/CA2445884A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne des articles et des procédés qui permettent de déterminer rapidement et efficacement la fonction de gènes. Des acides nucléiques sont disposés sur un substrat. Selon certains modes de réalisation préférés, on relie ensuite des cellules viables au substrat au niveau des points occupés par les acides nucléiques. Une transduction ou une transfection ultérieure des cellules par les acides nucléiques sans perte de vitalité des cellules permet d'exprimer les protéines codées par les acides nucléiques respectifs. Connaissant l'identité des acides nucléiques, du moins d'après leurs emplacements sur le substrat, il est possible de déterminer la fonction protéique correspondante. Sont décrits des procédés permettant de créer et d'utiliser de tels ensembles de cellules ainsi que des procédés de transfection et de transduction inverses.
CA002445884A 2001-04-30 2002-04-30 Elucidation de la fonction de genes Abandoned CA2445884A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28733501P 2001-04-30 2001-04-30
US60/287,335 2001-04-30
PCT/US2002/013432 WO2002088165A1 (fr) 2001-04-30 2002-04-30 Elucidation de la fonction de genes

Publications (1)

Publication Number Publication Date
CA2445884A1 true CA2445884A1 (fr) 2002-11-07

Family

ID=23102449

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002445884A Abandoned CA2445884A1 (fr) 2001-04-30 2002-04-30 Elucidation de la fonction de genes

Country Status (3)

Country Link
EP (1) EP1390382A4 (fr)
CA (1) CA2445884A1 (fr)
WO (1) WO2002088165A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070111201A1 (en) * 2001-04-30 2007-05-17 Benjamin Doranz Reverse transfection of cell arrays for structural and functional analyses of proteins
JP2006522605A (ja) * 2003-03-04 2006-10-05 独立行政法人産業技術総合研究所 経時的細胞解析法
EP2010907A1 (fr) * 2006-04-20 2009-01-07 Tethis S.r.l. Procede de detection de molecules biologiques presentes dans des cellules

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5200051A (en) * 1988-11-14 1993-04-06 I-Stat Corporation Wholly microfabricated biosensors and process for the manufacture and use thereof
US5751629A (en) * 1995-04-25 1998-05-12 Irori Remotely programmable matrices with memories
GB9815819D0 (en) * 1998-07-22 1998-09-16 Secr Defence Transferring materials into cells and a microneedle array
US6399305B1 (en) * 1999-06-07 2002-06-04 Fuji Photo Film Co., Ltd. Protection of partial complementary nucleic acid fragment using a electroconductive chip and intercalator

Also Published As

Publication number Publication date
EP1390382A1 (fr) 2004-02-25
WO2002088165A1 (fr) 2002-11-07
EP1390382A4 (fr) 2007-10-31

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued