CA2431778A1 - Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen - Google Patents

Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen Download PDF

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Publication number
CA2431778A1
CA2431778A1 CA 2431778 CA2431778A CA2431778A1 CA 2431778 A1 CA2431778 A1 CA 2431778A1 CA 2431778 CA2431778 CA 2431778 CA 2431778 A CA2431778 A CA 2431778A CA 2431778 A1 CA2431778 A1 CA 2431778A1
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CA
Canada
Prior art keywords
test
immunoassay
zone
hiv1
antigen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2431778
Other languages
French (fr)
Inventor
Zhigang Wang
Ying Liang Tian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
MEDISTAR BIOTECH Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MEDISTAR BIOTECH Inc filed Critical MEDISTAR BIOTECH Inc
Priority to CA 2431778 priority Critical patent/CA2431778A1/en
Publication of CA2431778A1 publication Critical patent/CA2431778A1/en
Abandoned legal-status Critical Current

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • G01N33/56988HIV or HTLV

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Description

Rapid Imunoassay for Multiple Detection of HIV 1+2 Antibodies and p24 Antigen1. A rapid cliagnostic immunoassay for multiple detection of HIV 1+2 antibodies and p24 antigen comprising:
a rapid test strip with an upstream end and a downstream end, wherein the test sample flows from upstream end towards downstream end, and wherein said strip has two test zones and one control zone with one said test zone contains HIV
1+2 antigen for detection of HIV 1+2 antibodies, the other said test zone contains McAb against p24 for detection of p24 antigen, and said control zone is for the validity checking of said immunoassay.
2. The immunoassay of claim l, wherein said test zones and control zone are arranged with the following order from said upstream end to downstream end:
(a) the HIVl+2 test zone, (b) the p24 test zone, and (c) the control zone.
3. The immunoassay of claim 1, wherein said test zones and control zone are arranged with the following order from said upstream end to downstream end:
(a) the p24 test zone, (b) the HIV 1+2 test zone, and (c) the control zone.
4. The immunoassay of claim 1, wherein said rapid immunoassay is colloidal gold immunochromatography assay.
5. The immunoassay of claim 1, 2, 3, and 4, wherein said immunoassay further comprising a housing which has openings and/or with transparent materials to receive said test samples and read the result from said test zones and control zone.

Claims (5)

1. A rapid diagnostic immunoassay for multiple detection of HIV1+2 antibodies and p24 antigen comprising:
a rapid test strip with an upstream end and a downstream end, wherein the test sample flows from upstream end towards downstream end, and wherein said strip has two test zones and one control zone with one said test zone contains HIV1+2 antigen for detection of HIV1+2 antibodies, the other said test zone contains McAb against p24 for detection of p24 antigen, and said control zone is for the validity checking of said immunoassay.
2. The immunoassay of claim 1, wherein said test zones and control zone are arranged with the following order from said upstream end to downstream end:
(a) the HIV1+2 test zone, (b) the p24 test zone, and (c) the control zone.
3. The immunoassay of claim 1, wherein said test zones and control zone are arranged with the following order from said upstream end to downstream end:
(a) the p24 test zone, (b) the HIV1+2 test zone, and (c) the control zone.
4. The immunoassay of claim 1, wherein said rapid immunoassay is colloidal gold immunochromatography assay.
5. The immunoassay of claim 1, 2, 3, and 4, wherein said immunoassay further comprising a housing which has openings and/or with transparent materials to receive said test samples and read the result from said test zones and control zone.
CA 2431778 2003-06-19 2003-06-19 Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen Abandoned CA2431778A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2431778 CA2431778A1 (en) 2003-06-19 2003-06-19 Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA 2431778 CA2431778A1 (en) 2003-06-19 2003-06-19 Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen

Publications (1)

Publication Number Publication Date
CA2431778A1 true CA2431778A1 (en) 2004-12-19

Family

ID=33557601

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2431778 Abandoned CA2431778A1 (en) 2003-06-19 2003-06-19 Rapid imunoassay for multiple detection of hiv1+2 antibodies and p24 antigen

Country Status (1)

Country Link
CA (1) CA2431778A1 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1798556A1 (en) * 2005-12-14 2007-06-20 The Jordanian Pharmaceutical Manufacturing Co. Device for the early and rapid immunochromatographic detection of HIV and uses thereof
EP1882943A1 (en) * 2006-07-27 2008-01-30 The Jordanian Pharmaceutical Manufacturing Co. Saliva immunochromatographic detection cup
CN102680690A (en) * 2011-03-17 2012-09-19 北京易斯威特生物医学科技有限公司 Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1798556A1 (en) * 2005-12-14 2007-06-20 The Jordanian Pharmaceutical Manufacturing Co. Device for the early and rapid immunochromatographic detection of HIV and uses thereof
WO2007068310A1 (en) * 2005-12-14 2007-06-21 The Jordanian Pharmaceutical Manufacturing Co. Device for the early and rapid immunochromatographic detection of hiv and uses thereof
EP1882943A1 (en) * 2006-07-27 2008-01-30 The Jordanian Pharmaceutical Manufacturing Co. Saliva immunochromatographic detection cup
WO2008011926A1 (en) * 2006-07-27 2008-01-31 The Jordanian Pharmaceutical Manufacturing Co. Saliva immunochromatographic detection cup
CN102680690A (en) * 2011-03-17 2012-09-19 北京易斯威特生物医学科技有限公司 Fourth-generation HIV (Human Immunodeficiency Virus) antibody antigen test paper, preparation method and application thereof

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