CA2426405A1 - Estrogen receptor alpha variants and methods of detection thereof - Google Patents

Abstract

The present invention is based on sequencing genomic DNA from human chromoso me 6 and cDNAs to define the genomic structure of estrogen receptor alpha genes and novel polymorphism/haplotypes in the estrogen receptor gene/protein. Suc h polymorphism/haplotypes can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorder, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences and sequence polymorphism/haplotypes in the ESR-alpha genes, methods of detecting these sequences/polymorphism/haplotype s in a sample, methods of determining a risk of having or developing a disorde r mediated by a variant estrogen receptor and methods of screening for compoun ds used to treat disorders mediated by a variant estrogen receptor.

Description

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ESTROGEN RECEPTOR ALPHA VARIANTS AND
METHODS OF DETECTION THEREOF
RELATED APPLICATIONS
The present application claims priority to applications U.S. Serial Nos.
09/692,414, filed October 20, 2000 (Atty. Docket CL000258); 09/768,184, filed January 24, 2001 (Atty.
Docket CL000258CIP); 09/804,076, filed March 13, 2001 (Atty. Docket CL000258CI2); and 09/826,314, filed April 5, 2001 (Atty. Docket CL000258CI3).
FIELD OF THE INVENTION
The present invention is in the field of disease detection and therapy. The present invention specifically provides the identification of previously unknown nucleic acid/amino acid polymorphisms within the estrogen receptor alpha gene (ESR-alpha) and the genomic sequence of this gene for use in the development of diagnostics and therapies for diseases and disorders mediated/modulated by the estrogen receptor.
BACKGROUND OF THE INVENTION
Estrogen Receptor The human estrogen receptor alpha belongs to the nuclear hormone receptor family.
Nuclear hormone receptors are a family of hormone-activated transcription factors that can initiate or enhance the transcription of genes containing specific hormone response elements.
The ER protein consists of 595 amino acids with a molecular weight of 66 kDa, transcribed exons, with six different functional domains. Two of those domains are highly conserved in the primaxy sequence of members of the nuclear hormone receptor superfamily.
One of the domains, the DNA binding domain (DBD), contains two zinc fingers that mediate receptor binding to hormone response elements in the promoters of hormone-responsive genes. In the C- terminal region, the hormone-binding domain (HBD) contains two regions of sequence homology with other hormone receptors and gives hormone specificity and selectivity. The human ER-alpha gene is located in chromosome 6q.25.1.
Estrogen receptors, like other steroid receptors, are transcription factors that are activated upon binding to steroids (estradiol) or steroid analogs such as tamoxifen. Upon activation the receptors dimerize to form homodimers or heterodimers that bind to estrogen receptor elements (EREs) located in the promoter region of estrogen-activated genes and coordinate transcription by interacting with host co-activators.

Role of Estrogen in Cardiovascular Disease , Heart disease is the leading cause of mortality in women, a fact that is under appreciated by both women and physicians. One in 9 women aged 45-65 have some form of cardiovascular disease and the number increases to 1 in 3 after age 65. Each year, 240,000 U.S. women die from heart disease, and nearly 90,000 die of stroke. Moreover, approximately 44% die within one year of suffering a heart attack, compared with 26% of men (Warren MP and Kulak J Clin Obs Gyn 1998 41 (4):976-987).
Estrogens exert a wide range of physiological effects on a large variety of cell types.
For example, they regulate cell growth and apoptosis and a myriad of functions related to reproduction. There are two types of estrogen receptors, alpha and beta. Blood vessels and bone contain beta receptors, the liver has alpha receptors, and both alpha and beta receptors are found in the central nervous system. The interaction of these different receptor sites influences the biological effects of estrogen and selective estrogen receptor modulators (SERMs), such as raloxifene. The binding patterns dictate whether an estrogen or a SERM
acts as an estrogen agonist or an antagonist (Mendelsohn ME and Karas RH New Engl J Med 1999, 340(23):1801-181 l;Grese TA and Dodge JA Curr Pharm Design 1998, 4:71-92).
Tissue-specific relationships exist between SERMs and the receptor binding sites. Estrogens also increase high-density lipoprotein cholesterol levels, decrease low-density lipoprotein cholesterol, and decrease plasminogen-activating inhibitor levels (Meisler JG
Jour Women's Health 1999, 8(1):51-57). All estrogens require cellular receptors for their expression. In general, estrogen receptors are ligand-inducible transcription factors, which regulate the expression of target genes after hormone binding (Faustini-Fustini et al. Eur J Endocrin 1999, 140:111-129). Estrogen may also have important effects on the vascular wall.
Estradiol and progesterone receptors have been identified in arterial endothelial and smooth muscle cells (Campisi D et al. Int J Tiss React 1987, IX(5):393-398). Estrogens act on the wall of the artery to relax vascular smooth muscle and to decrease vascular resistance.
The mechanism appears to be through stimulation of endothelial-derived relaxing factors and an endogenous nitrate (Warren MP and Kulak J Clin Obs Gyn 1998 41 (4):976-987). The relaxation induced by 17B-estradiol may play an important role in the regulation of coronaxy tone, which reduces the risk of coronary disease in postmenopausal women. The production of nitric oxide is mediated by the estrogen receptor, because when the receptor is blocked by an antiestrogen agent, nitric oxide is suppressed.

Several studies have shown that estrogen therapy reduces the risk of heart disease by up to 50% (most recently reviewed by Mendelsohn ME and Karas RH New Engl J Med 1999, 340(23):1801-181 l;Rich-Edwards JW N Engl J Med, 1995, 332:1758-1765;
Gerhard M, Ganz P, Circulation, 1995, 92:5-8; Grodstein F, et al N Engl J Med 1997, 336:1769-75;
Chasen-Taber L and Stampfer MJ Ann of Int Med, 1998, 128:467-477; Warren MP
and I~ulak J Clin Obstet Gyn 1998, 41 (4):976-987). Loss of estrogen may be one of the most important factors in the development of cardiovascular disease in women.
While there is no direct evidence that estrogen prevents atherogenesis, considerable epidemiologic evidence exists that suggests that estrogens may have some benefit in reducing cardiovascular disease: (1) In all age groups, women have a lower incidence of cardiovascular disease than do men; (2) women who undergo a premature surgical menopause and do not take estrogens are twice as likely to have cardiovascular disease are age-matched premenopausal controls; (3) postmenopausal women who use estrogens have a significantly lower incidence of cardiovascular disease compared with those who do not; and (4) women with coronary artery disease detected by angiography have a higher survival rate if they are estrogen users.
In recent years, reports of favorable effects of estrogen therapy on cardiovascular morbidity and mortality have led to enthusiasm for widespread use of estrogens by postmenopausal women (Meinertz T Herz 1997, 22: 151-157). Guidelines for estrogen therapy issued by the American College of Physicians include the statement "Women who have coronary heart disease are likely to benefit from hormone therapy."
More than 30 prospective studies and 13 case controlled studies have examined the effect of estrogen replacement therapy on cardiovascular incidence or prevalence and all cause mortality (Stampfer MJ et al. New Engl J Med 1991, 325:756-62; Grady D
et al. Ann Intern Med 1992, 117:1016-37). The majority of these studies showed lower morbidity and mortality from coronary heart disease among users of postmenopausal estrogens than among non-users. Specifically, they have shown that coronary artery disease in estrogen takers is approximately 50% that in women who do not take estrogen. Overall, the bulk of the evidence strongly supports a protective effect of estrogens yielding a relative risk of 0.56 (95% confidence interval 0.50-0.61). However, a "healthy woman selection bias"
is present in these studies and potentially may confound these results (estrogen takers have better weight control, exercise more, and smoke less than women who are not prescribed estrogen).

Moreover, other biases such as estrogen takers tend to have higher education, higher income, etc., are confounding these epidemiologic studies (Abrams J Clin Cardiol 1998, 21:218-222).
Since the earlier observational trials were not randomized, it is believed by many that as much as 25% of this 50% reduction in risk is due to these various methodological biases (Barrett-Conner E and Grady D 1998, Ann Rev Public Health 19:55-72). Recently, 2 meta-analyses estimated the reduction in coronary heart disease associated with estrogen use to be in the range of 35 to 44 %, respectively (Grodstein F and Stampfer MJ Prog Cardiol Dis 1995, 38: 199-210; Barrett-Conner E and Grady D 1998, Annu Rev Public Health 19:55-72).
Recent studies are exploring the issue of opposed vs unopposed estrogen, because of a documented increased risk for uterine cancer in women with an intact uterus who are taking estrogen alone. The new lines of evidence are suggesting that women taking estrogen plus a progestin (usually a medroxyprogesterone acetate) do not receive an equivalent benefit from the cardioprotective effects compared to women taking estrogen alone (Hulley S
et al 1998 JAMA 280:605-613; Abrams J Clin Cardiol 1998, 21:218-222).
The loss of estrogen at menopause is associated with a 6% decline in HDL
cholesterol levels and a 5% rise in LDL cholesterol levels, which may explain the higher cardiovascular disease rate among postmenopausal women compared with premenopausal women. The lower incidence of cardiovascular disease among postmenopausal women who take estrogen may be explained in part by the resultant 15% to 19% decrease in LDL
cholesterol levels and the 16% to 18% increase in HDL cholesterol levels (JAMA 1995, 273:199-208).
The PEPI
(Postmenopausal Estrogen/Progestin Intervention, a randomized, double-blind placebo-controlled trial, showed that HDL cholesterol levels rose significantly more in women assigned to estrogen alone than in women assigned the combined estrogen (JAMA
1995, 273:199-208). Recent non-human primates studies substantiate these findings (Clarkson TB
Lab An Sci 1998, 48(6):569-72). Statistical modeling of the effect of estrogen on lipid profiles indicates that 25 - 50% of the apparent cardioprotection due to estrogen is mediated by favorable changes in HDL-cholesterol (Bush TL et al. 1987 Circulation 75:1102-9;
Gruchow HW et al. 1988 Am Heart J 115:954-63).
Estrogen replacement therapy is not without risk. For years, studies have shown a 3-4-fold increased risk of venous thromboembolism (VTE) in users of oral contraceptives compared to non-users (Weiss G Am J Obstet Gynecol 1999 180:5295-301). One study has shown that intrinsic coagulation factors play a significant role in oral contraceptive-associated VTE (Vandenbrouclce JP et al. Lancet 1994 344:1453-7; Rosing J et al. Br J

Haematol 1997, 97:233-238). The Factor V Leiden mutation increases risk of VTE
5-10 fold in non users, but 30-fold in third-generation oral contraceptive users.
Combined estrogens appear to induce resistance to the body's natural anticoagulation system (APC).
Heterozygotes for the Factor V Leiden mutation who take oral contraceptives develop APC
resistance as high as that seen in women who are homozygous.
Estrogens increase the risk of endometrial carcinoma approximately 6-fold, an effect that is eliminated, for the most part, by the addition of progestins (Baxrett-Conner E and Grady D 1998, Ann Rev Public Health 19:55-72). Controversy continues over whether estrogen replacement increases the risk of breast cancer, but some studies indicate risk is elevated by as much as 30%. (Greendale GA et al. Lancet 1999, 353:571-80).
A number of prospective randomized studies designed to definitely establish whether estrogen replacement therapy reduces the risk of cardiovascular disease in women and whether it increases the risk of breast cancer, are underway. One recently completed trial (HERS - Heart and Estrogen/progestin Replacement Study) compared continuous combined estrogen plus medroxyprogesterone acetate to placebo in 2700 women with pre-existing coronary disease (Hully S et al. 1998 JAMA 280(7):605-13). Compared to controls, the intervention group had significantly more heart disease events- in year one of the trial, but significantly fewer events in years 4 and 5 of the trial. Moreover, a significant increase in the rate of thromboembolic events occurred in the early years of the study in women taking hormones. Based on these results, hormone replacement therapy is not recommended for secondary prevention of heart disease.
Two other large, ongoing clinical trials on primary prevention of cardiovascular disease using estrogens are underway. The Women's Health Initiative, due to be completed in 2005 and a U.K. study called WIS-DOM, due to be completed in 2010, should shed new light on the protective effects of estrogen on cardiovascular disease (Meisler JG Jour Women's Health 1999, 8(1):51-5).
In summary, ongoing research suggests that estrogen replacement therapy, particularly involving recently formulated designer estrogens or SERMs, may have beneficial effects on the cardiovascular system as well as bone, without the untoward effects on breast and endometrial tissue. Caution still needs to be observed, nonetheless. Women who take estrogens are, on average, better educated, healthier, have higher incomes and have better access to health care. These differences rather than the estrogens may account for much of the lower risk of heart disease.

For postmenopausal women without frank disease, estrogen replacement therapy appears to have a beneficial effect when one considers the magnitude, consistency, and biological plausibility of the data. For women with pre-existing disease, questions remain as to the safety and efficacy of exogenous estrogens as protective agents against cardiovascular disease.
Estrogen and autoimmune diseases A. S~~stemic Lupus Erythematosus There is a widely held view that estrogens play a role in Systemic lupus erythematosus because:
1. Women of child bearing age are nine times more likely to develop systemic lupus erythematosus than men. Prior to pubescence the rate is three fold higher in females, while post menopausal women have an equal chance of developing SLE as aged matched males.
Many studies have been done that show that the reason for the differences in the sexes is probably estrogen related (Lahita R.G., 1986: Springer Seminars in Immunopathology 9, 305-314; Krammer, G.M. and Tsokos, G.C. , 1998 Clinical Immunology and Immunopathology 89: 192-195; Rider at al., 1998 Clinical Immunology and Immunopathology 89: 171-180).
Clues to the role of estrogens in SLE came from studies that concluded that oral contraceptives adversely affected the morbidity of this illness (Buton, J.P., 1996 Ann. Med.
Interne, 147:259-264; Julkunen, I99I: Scan. J. Rheumatol. 20:427=433).
2. Patients with Klinefelter syndrome (XXY), have been reported with SLE
(Stern et al., 1977: Arthritis and Rheumatism 20:18-22).
3. Patients with SLE have anti-estrogen antibodies (Feldman, 1987: Biochem.
Biophys. Acta, 145:1342-1348: Bucala et al., 1987: Clin. Exp. Immunol. 67:167-175) In the past, oral contraceptives have been shown to cause flare ups of SLE, their use was discouraged in women with SLE, while the current thinking is that the lower dose birth control pills are safe for SLE patients (Julkunen HA Scand JRheumatol 1991;20(6):427-33).
As well hormone replacement therapy is considered safe for SLE patients (Mok et al., Scand JRheurnatol 1998;27(5):342-6: Kreidstein et al., 1997, JRheunaatol 1997 Nov;24(11):2149-52) 4. The estrogen antagonist tamoxofm seems to improve the course of the disease (Sthoeger, 1997, Ahh N YAcad Sci 1997 Apr 5;815:367-8: Sthoeger, 1994, JRheummatol 1994 Dec;21(12):2231-8).
B. Estrogen, Rheumatoid Arthritis (RA) and osteoarthritis The literature surrounding the involvement of estrogens in Rheumatoid arthritis is less clear than with osteoarthritis. Epidemiological studies suggests that RA is influenced by female sex hormones, by one study states that the use of oral contraceptives may postpone the onset of RA, but that. estrogens alone no not alleviate the symptoms of RA
(Bijlsma Am J
Reprod Immu~ol 1992 Oct-Dec;28(3-4):231-4). Adjuvant oestrogen treatment does increase bone mineral density in postmenopausal women with RA, and may protect against osteophoresis which is often a complication of RA (van den Brink: Anh Rheum Dis 1993 Apr;52(4):302-5). While the study mentioned above indicated that estrogens did not alleviate RA symptoms, another study concluded that adjuvant estrogen therapy did not even improve the symptoms. One polymorphism has been reported in the estrogen receptor that seems to be associated with the age of onset of RA (Ushiyama Anh Rheum Dis 1999 Jan;58(1):7-10) Osteoarthritis on the other hand is less prevalent in postmenopausal women who take estrogen replacement therapy (ERT) (Felson Curs Opin Rheumatol 1998 May;lO(3):269-72) suggesting that ERT may be beneficial in preventing osteoarthritis.
C. Estrogen and Osteo op rosin Osteoporosis is a metabolic bone disorder that leads to bone fragility and subsequent risk of fracture. Treatment for postmenopausal women with osteoporesis includes hormone replacement, in particular estrogen. Estrogen has shown to reduce the incidence of bone loss and fractures (Weiss et al., NEngl JMed 1980 Nov 20;303(21):1195-8 :Paganini-Hill et al., Any Intern Med 1981 Ju1;95(1):28-31: Ettinger et al., Ann Inters Med 1985 Mar;102(3):319 24) Further, polymorphisms in the estrogen receptor have been associated with bone loss in both humans and mice.( Kobayashi JBohe Mirae~ Res 1996 Marl 1(3):306-11 Kurabayashi Am J Obstet Gynecol 1999 May;180(5):1115-20; Deng Hum Genet 1998 Nov;103(5):576-85 ) Estro .ens and Cognitive function Compared with men, women are at greater risk of developing Alzheimer's disease.
Several studies show that women who take estrogen after menopause have a lower incidence of Alzheimer's disease. Among women with Alzheimer's, those taking estrogen suffer less severe symptoms and slower mental deterioration. The duration of estrogen use also seems to be important in reducing risk. Women with a history of long-term use (more than 10 years) had the lowest risk. But even women who took estrogen for a short time also benefited.
Estrogen and breast cancer The major risk factors for the development of breast cancer axe sex, age, family history of breast cancer, age of menarche, age at first full-term pregnancy, and age of menopause. All of these factors, with the exception of family history, have been shown to be directly associated with lifetime exposure to estrogen, increased hormone exposure being associated with increased risk of developing breast cancer. The increased cancer risk is believed to be caused by an estrogen receptor-mediated proliferative response in cells of the mammary epithelium.
Tamoxifen, an estrogen receptor antagonist, has been shown to be an effective agent for both the prevention and treatment of breast cancer. Using immunohistochemical methods, it is possible to classify breast tumors as being estrogen receptor positive or negative, depending upon the amount of estrogen receptor protein expressed in the tissue. Estrogen receptor positive tumors are more likely to respond to treatment with tamoxifen than estrogen receptor negative tumors. Pre-menopausal women are more likely to develop estrogen receptor negative breast cancers than are post-menopausal women.
Mutations altering the structure and function of the estrogen receptor have been described in primary breast tumors or breast cancer cell lines. It is not clear however whether these changes are primary (and involved in the processes leading to carcinogenesis) or secondary (and a consequence of genetic instability in cancer tissues). In addition to these somatic mutations, some studies have pointed to a possible association between inherited DNA sequence changes and the development of breast cancer, but these studies are also controversial.
Further evidence for the role of estrogen receptors in breast cancer comes from the recent fording that the gene BRCAl, which when inherited in a mutant form predisposes to 3 0 the development of breast cancer, inhibits estrogen receptor signaling.

Estro~ens and endometrial cancer Carcinoma of the endometrium is the most common pelvic malignancy in women, however because in approximately 75% of cases it is confined to the body of the uterus at the time of diagnosis, it can usually be cured by hysterectomy. Unopposed exposure of endometrial cells to estrogens dramatically increases the chance of developing this form of uterine cancer and it is for this reason that hormone replacement therapy consisting solely of estrogen should not be given to women with intact uteri. Cyclical or continuous co-administration of progesterone serves to prevent excessive proliferation of endometrial cells, reducing the risk of endometrial cancer in post-menopausal women receiving estrogen as part of hormone replacement therapy regimens.
The majority of cases of endometrial cancers express estrogen receptor and, in general, estrogen responsive tumors have a favorable prognosis. Acquired (somatic) mutations have been described in up to 8.5% of cases, however the role of these mutations in the development and progression of endometrial cancer is uncertain at present.
Although it remains somewhat controversial, studies suggest that use of tamoxifen may increase the chance of developing endometrial cancer. This may be because, in addition to its role in estrogen receptor blockade, tamoxifen has partial receptor agonist activity and results in low-grade induction of estrogen responsive genes that induce endometrial proliferation.
Given the involvement of the estrogen receptor in mediating/modulating various disorders, it is critical to identify sequence polymorphisms in the estrogen receptor and to correlate these with disease states, therapeutic effectiveness and the like.
The present invention advances the art by providing a variety of previously unidentified polymorphisms in the ESR-alpha protein.
SNPs The genomes of all organisms undergo spontaneous mutation in the course of their continuing evolution, generating variant forms of progenitor sequences (Gusella, Ann. Rev.
Biochem. 55, 831-854 (1986)). The variant form may confer an evolutionary advantage or disadvantage relative to a progenitor form or may be neutral. In some instances, a variant form confers a lethal disadvantage and is not transmitted to subsequent generations of the organism. In other instances, a variant form confers an evolutionary advantage to the species and is eventually incorporated into the DNA of many or most members of the species and effectively becomes the progenitor form. Additionally, the effect of a variant form may be both beneficial and detrimental, depending on the circumstances. For example, a heterozygous sickle cell mutation confers resistance to malaria, but a homozygous sickle cell mutation is usually lethal. In many instances, both progenitor and variant forms) survive and co-exist in a species population. The coexistence of multiple forms of a sequence gives rise to polymorphisms, such as SNPs.
The reference allelic form is arbitrarily designated and may be, for example, the most abundant form in a population, 'or the first allelic form to be identified, and other allelic forms are designated as alternative, variant or polymorphic alleles. The allelic form occurring most frequently in a selected population is sometimes referred to as the "wild type" form.
Approximately 90% of all polymorphisms in the human genome are single nucleotide polymorphisms (SNPs). SNPs axe single base pair positions in DNA at which different alleles, or alternative nucleotides, exist in some population. The SNP
position, or SNP site, is usually preceded by and followed by highly conserved sequences of the allele (e.g., sequences that vary in less than 1/100 or 1/1000 members of the populations).
An individual may be homozygous or heterozygous for an allele at each SNP position. As defined by the present invention, the least frequent allele at a SNP position can have any frequency that is less than the frequency of the more frequent allele, including a frequency of less than 1 % in a population. A SNP can, in some instances, be referred to as a "cSNP" to denote that the nucleotide sequence containing the SNP is an amino acid coding sequence.
A SNP may arise due to a substitution of one nucleotide for another at the polymorphic site. Substitutions can be transitions or transversions. A
transition is the replacement of one purine nucleotide by another purine nucleotide, or one pyrimidine by another pyrimidine. A transversion is the replacement of a purine by a pyrimidine, or vice versa. A SNP may also be a single base insertion/deletion variant (referred to as "indels"). A
substitution that changes a codon coding for one amino acid to a codon coding for a different amino acid is referred to as a non-synonymous codon change, or missense mutation. A
synonymous codon change, or silent mutation, is one that does not result in a change of amino acid due to the degeneracy of the genetic code. A nonsense mutation is a type of non-synonymous codon change that results in the formation of a stop codon, thereby leading to 3 0 premature termination of a polypeptide chain and a defective protein.
SNPs, in principle, can be bi-, tri-, or tetra- allelic. However, tri- and tetra-allelic polymorphisms are extremely rare, almost to the point of non-existence (Brookes, Gene 234 (1999) 177-186). For this reason, SNPs are often referred to as "bi-allelic markers", or "di-allelic markers".
Causative SNPs are those SNPs that produce alterations in gene expression or in the expression or function of a gene product, and therefore are most predictive of a possible clinical phenotype. One such class includes SNPs falling within regions of genes encoding a polypeptide product, i.e. cSNPs. These SNPs may result in an alteration of the amino acid sequence of the polypeptide product (i.e., non-synonymous codon changes) and give rise to the expression of a defective or other variant protein. Furthermore, in the case of nonsense mutations, a SNP may lead to premature termination of a polypeptide product.
Such variant products can result in a pathological condition, e.g., genetic disease.
Examples of genes in which a polymorphism within a coding sequence gives rise to genetic disease include sickle cell anemia and cystic fibrosis. Causative SNPs do not necessarily have to occur in coding regions; causative SNPs can occur in any region that can ultimately affect the expression and/or activity of the protein encoded by the nucleic acid. Such gene areas include those involved in transcription, such as SNPs in promoter regions, in gene areas involved in transcript processing, such as SNPs at intron-exon boundaries that may cause defective splicing, or SNPs in mRNA processing signal sequences such as polyadenylation signal regions. For example, a SNP may inhibit splicing of an intron and result in mRNA containing a premature stop codon, leading to a defective protein. Consequently, SNPs in regulatory regions can have substantial phenotypic impact.
Some SNPs that are not causative SNPs nevertheless are in close association with, and therefore segregate with, a disease-causing sequence. In this situation, the presence of the SNP correlates with the presence of, or susceptibility to, the disease. These SNPs are invaluable for diagnostics and disease susceptibility screening.
Clinical trials have shown that patient response to treatment with pharmaceuticals is often heterogeneous. Thus there is a need for improved approaches to pharmaceutical agent design and therapy. SNPs can be used to help identify patients most suited to therapy with particular pharmaceutical agents (this is often termed "pharmacogenomics") Pharmacogenomics can also be used in pharmaceutical research to assist the drug selection process. (Linder et al. (1997), Clinical Chemistry, 43, 254; Marshall (1997), Nature Biotechnology, 15, 1249; International Patent Application WO 97/40462, Spectra Biomedical; and Schafer et al. (I998), Nature Biotechnology, 16, 3.).

Population Studies Population Genetics is the study of how Mendel's laws and other genetic principles apply to entire populations. Such a study is essential to a proper understanding of evolution because, fundamentally, evolution is the result of progressive change in the genetic composition of a population. Population genetics thus seeks to understand and to predict the effects of such genetic phenomena as segregation, recombination, and mutation;
at the same time, population genetics must take into account such ecological and evolutionary factors as population size, patterns of mating, geographic distribution of individuals, migration and natural selection.
Ideally, one would wish to know how to describe the types and frequencies of genes in a population, to explain how the population's genetic composition came to be the way it is, and to predict how the population would change as a result of natural selection or as a result of artificial selection.
In order to explain many of those issues it is important to understand the existing relation between loci denominated: Linkage.
Linkage is the coinheritance of two or more nonallelic genes because their loci are in close proximity on the same chromosome, such that after meiosis they remain associated more often than the 50% expected for unlinked genes. During meiosis, there is a physical crossing over, it is clear that during the production of germ cells there is a physical exchange of maternal and paternal genetic contributions between individual chromatids.
This exchange necessarily separates genes in chromosomal regions that were contiguous in each parent and, by mixing them with retained linear order, results in "recombinants". The process of forming recombinants through meiotic crossing-over is an essential feature in the reassortment of genetic traits and is central to understanding the transmission of genes.
Recombination generally occurs between large segments of DNA. This means that contiguous stretches of DNA and genes are likely to be moved together.
Conversely, regions of the DNA that are far apart on a given chromosome are likely to become separated during the process of crossing-over.
It is possible to use molecular maxkers to claxify the recombination events that take place during meiosis. Some markers as (CA)n repeats of different lengths are dispersed throughout human DNA and there is little selective pressure in their lengths axe used as position markers and regional identifying characters along chromosomes. Those markers can be used to distinguish paternally derived from maternally derived gene regions.
Other markers are Single Nucleotide Polymorphism (SNP), those are biallelic markers, also used to analyzed the transmission of those markers to offspring.
The pattern of a set of markers along a chromosome is referred to as a "Haplotype".
Therefore sets of alleles on the same small chromosomal segment tend to be transmitted as a block through a pedigree. By analyzing the haplotypes in a series of offspring of parents whose haplotypes are known, it is possible to establish which parental segment of which chromosome was transmitted to which child. When not broken up by recombinations, haplotypes can be treated for mapping purposes as alleles at a single highly polymorphic locus.
The existance of a preferential occurrence of a disease gene in association with specific alleles of linked markers is called "Linkage Disequilibrium"(LD).
This sort of disequilibrium generally implies that most of the disease chromosomes carry the same mutation and the markers being tested are quite close to the disease gene. Fox example, there is considerable linkage disequilibrium across the entire HLA locus. The A3 allele is in LD
with the B7 and B14 alleles, and as a result B7 and B14 are also highly associated with hemochromatosis. Thus, HLA typing alone can significantly alter the estimate of risk for hemochromatosis, even if other family members are not available for formal linkage analysis.
As a result, using a combination of several markers surrounding the presumptive location of the gene, a haplotype can be determined for affected and unaffected family members.
SNP-Based Association Analysis and Linkage Disequilibrium Mappin SNPs are useful in association studies for identifying particular SNPs, or other polymorphisms, associated with pathological conditions, such as breast cancer.
Association studies may be conducted within the general population and are not limited to studies performed on related individuals in affected families (linkage studies). An association study using SNPs involves determining the frequency of the SNP allele in many patients with the disorder of interest, such as breast cancer, as well as controls of similar age and race. The appropriate selection of patients and controls is critical to the success of SNP association studies. Therefore, a pool of individuals with well-characterized phenotypes is extremely desirable. For example, blood pressure and heart rate can be correlated with SNP patterns in hypertensive individuals in whom these physiological parameters are known in order to find associations between particular SNP genotypes and known phenotypes.
Significant associations between particular SNPs or SNP haplotypes and phenotypic characteristics can be determined by standard statistical methods. Association analysis can either be direct or LD
based. In direct association analysis, causative SNPs are tested that are candidates for the pathogenic sequence itself.
In LD based SNP association analysis, random SNPs are tested over a large genomic region, possibly the entire genome, in order to find a SNP in LD with the true pathogenic sequence or pathogenic SNP. For this approach, high density SNP maps are required in order for random SNPs to be located close enough to an unknown pathogenic locus to be in linkage disequilibrium with that locus in order to detect an association. SNPs tend to occur with great frequency and are spaced uniformly throughout the genome. The frequency and uniformity of SNPs means that there is a greater probability, compared with other types of polymorphisms such as tandem repeat polymorphisms, that a SNP will be found in close proximity to a genetic locus of interest. SNPs are also mutationally more stable than tandem repeat polymorphisms, such as VNTRs. LD-based association studies are capable of finding a disease susceptibility gene without any a priori assumptions about what or where the gene is.
Currently, however, it is not feasible to do SNP association studies over the entire human genome, therefore candidate genes associated with breast cancer are targeted for SNP
identification and association analysis. The candidate gene approach uses a priori knowledge of disease pathogenesis to identify genes that are hypothesized to directly influence development of the disease. The candidate gene approach may focus on a gene that is directly targeted by a drug used to treat the disorder. To discover SNPs associated with an increased susceptibility to breast cancer, candidate genes can be selected from systems physiologically implicated in the disease pathway. SNPs found in these genes are then tested for statistical association with disease in individuals who have the disease compared with appropriate controls. The candidate gene approach has the.advantages of drastically reducing the number of candidate SNPs, and the number of individuals, that need to be typed, compaxed with LD-based association studies of random SNPs over large areas of, or complete, genomes. Furthermore, in the candidate gene approach, no assumptions are made about the extent of LD over any particular area of the genome.
Combined with the use of a high density map of appropriately spaced, sufficiently informative SNP markers, association studies, including linkage disequilibrium-based genome wide association studies, will enable the identification of most genes involved in complex disorders, such as breast cancer. This will enhance the selection of candidate genes most likely to contain causative SNPs associated with a particular disease.
All of the SNPs disclosed by the present invention can be employed as part of genome-wide association studies or as part of candidate gene association studies.
The present invention advances the state of the art and provides commercially useful embodiments by providing previously unidentified SNPs in the estrogen receptor genes.
SUMMARY OF THE INVENTION
The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor alpha genes, novel polymorphisms in the estrogen receptor gene/protein and previously unknown haplotypes. Such polymorphisms/haplotypes can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorders, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences, sequence polymorphisms in the ESR-alpha gene, haplotypes of these polymorphisms, methods of detecting these sequences/polymorphisms in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.
DESCRIPTION OF THE FIGURES
Figure 1. Complete genomic sequence of the estrogen receptor alpha gene.
Figure 2. Sequence polymorphisms found in the ESR-alpha genomic DNA
(nucleotide position is based on the sequence provided in Figure 1.) (a) SNPs in Liverpool clinical tissue samples.
(b) SNPs in Coriell Diversity panels.
(c) SNPs in Liverpool Control Population (d) PCR primers.
(e) Sequencing primers.
Figure 3. Amino acid sequence of the estrogen receptor alpha protein.
Figure 4. Estrogen Receptor Haplotypes (See Haplotype Section).
(a) Liverpool samples from 48 patients, and each patient had a tumor and blood sample typed. Coriell samples were control.

(b) The non-singleton haplotype data fitted to a neighbor-joining tree (L is Liverpool sample).
Figure 5. The domain structure of the ESRl protein and the positions of the SNPs disclosed herein.
Figure 6. The distribution and frequency of many of the SNPs of the present invention.
Figure 7. A graphic representation of the human ESR1 locus.
(a) Complete structure of the human estrogen receptor alpha (ERcc). Exons are represented by filled boxes and introns by horizontal lines.
(b) Order and names of contigs used to complete the genomic sequence. GA
numbers represent Celera contig numbers. Research Genetics BAC clones are represented by standard plate and well numbering.
Figure 8. ESR-alpha SNPs Genotyping Results a) in Coriell Samples, b) in Liverpool Samples (T= tumor sample, B= blood sample, LC=Liverpool controls), c) in Liverpool Control sample Figure 9. ESR-alpha exons with SNPs. (see Figure 2 for "N", "C", "I", "A", "S"
representations). Underlined sequences indicate the primer sequences.
DETAILED DESCRIPTION OF THE INVENTION
General Description The present invention is based on sequencing genomic DNA from human chromosome 6 and cDNAs to define the genomic structure of estrogen receptor alpha genes and novel polymorphisms and haplotypes in the estrogen receptor gene/protein.
Such polymorphisms/haplotypes can lead to a variety of disorders that are mediated/modulated by a variant estrogen receptor, such as a susceptibility to cancer, osteoporosis, cardiovascular disorders, etc. Based on this sequencing approach, the present invention provides genomic nucleotide sequences, cDNA sequences, amino acid sequences and sequence polymorphisms/haplotypes in the ESR-alpha gene, methods of detecting these sequences/polymorphisms/haplotypes in a sample, methods of determining a risk of having or developing a disorder mediated by a variant estrogen receptor and methods of screening for compounds used to treat disorders mediated by a variant estrogen receptor.
Isolated SNP-Containing Nucleic Acid Molecules The present invention provides isolated nucleic acid molecules that contain one or more SNPs disclosed by the present invention. The present invention further provides isolated nucleic acid molecules that encode the variant protein. Such nucleic acid molecules will consist of, consist essentially of, or comprise one or more SNPs of the present invention.
The nucleic acid molecule can have additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences.
As used herein, an "isolated" SNP-containing nucleic acid molecule is one that contains a SNP of the present invention and is separated from other nucleic acid present in the natural source of the nucleic acid. Generally, the isolated SNP-containing nucleic acid, as used herein, will be comprised of one or more SNP positions disclosed by the present invention with flanking nucleotide sequence on either side of the SNP positions. Preferably the flanking sequence is up to about 300 bases, 100 bases, 50 bases, 30 bases, 15 bases, I O bases, or 4 bases on either side of a SNP position for detection reagents or as long as the entire protein encoding sequence if it is to be used to produce a protein containing the coding variants disclosed in Figures. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences and is of appropriate length such that it can be subjected to the specific manipulations or uses described herein such as recombinant expression, preparation of probes and primers for the SNP position, and other uses specific to the SNP-containing nucleic acid sequences.
Moreover, an "isolated" nucleic acid molecule, such as a cDNA molecule containing a SNP of the present invention, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated. For example, recombinant DNA
molecules contained in a vector are considered isolated. Further examples of isolated DNA
molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA
molecules include ih vivo or in vitro RNA transcripts of the isolated SNP-containing DNA
molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.
Isolated SNP-containing nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the non-coding strand (anti-sense strand).

The present invention further provides related nucleic acid molecules that hybridize under stringent conditions to the nucleic acid molecules disclosed herein. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 60-70% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 60%, at least about 70%, or at least about 80%, or at least about 90% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6X sodi .un chloride/sodium citrate (SSC) at about 45 °C, followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-65 °C. Examples of moderate to low stringency hybridization conditions are well known in the art.
~ecific Embodiments Peptide Molecules The present invention provides nucleic acid sequences that encode variants of the estrogen receptor. These variant molecule/sequences will be referred to herein as the estrogen receptor variants of the present invention, the estrogen receptor proteins of the present invention, or peptides/proteins of the present invention.
The present invention provides isolated estrogen receptor protein molecules that consist of, consist essentially of or are comprised of the amino acid sequences of the estrogen receptor variant proteins disclosed herein.
As used herein, a protein or peptide is said to be "isolated" or "purified"
when it is substantially free of cellular material or free of chemical precursors or other chemicals. The peptides of the present invention can be purified to homogeneity or other degrees of purity. The level of purification will be based on the intended use. The critical feature is that the preparation allows for the desired function of the peptide, even if in the presence of considerable amounts of other components.
In some uses, "substantially free of cellular material" includes preparations of the peptide having less than about 30% (by dry weight) other proteins (i.e., contaminating protein), less than about 20% other proteins, less than about 10% other proteins, or less than about 5% other proteins. When the peptide is recombinantly produced, it can also be substantially free of culture medium, i.e., culture medium represents less than about 20% of the volume of the protein preparation.
The language "substantially free of chemical precursors or other chemicals"
includes preparations of the peptide in which it is separated from chemical precursors or other chemicals that are involved in its synthesis. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of the estrogen receptor protein having less than about 30% (by dry weight) chemical precursors or other chemicals, less than about 20% chemical precursors or other chemicals, less than about 10% chemical precursors or other chemicals, or less than about 5% chemical precursors or other chemicals.
The isolated estrogen receptor proteins can be purified from cells that naturally express it, purified from cells that have been altered to express it (recombinant), or synthesized using known protein synthesis methods. For example, a nucleic acid molecule encoding the estrogen receptor protein is cloned into an expression vector, the expression vector introduced into a host cell and the protein expressed in the host cell. The protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Many of these techniques are described in detail below.
Accordingly, the present invention provides proteins that consist of the amino acid sequences summarized in Figure 1, including one or more of the sequence polymorphisms provided in Figure 2. A protein consists of an amino acid sequence when the amino acid sequence is the final amino acid sequence of the protein.
The present invention further provides proteins that consist essentially of the amino acid sequences summarized in Figure 1, including one or more of the sequence polymorphisms provided in Figure 2. A protein consists essentially of an amino acid sequence when such an amino acid sequence is present with only a few additional amino acid residues in the final protein.
The present invention fiufiher provides a protein that is comprised of the amino acid sequences summarized in Figure 1, including one or more of the sequence polymorphisms provided in Figure 2. A protein is comprised of an amino acid sequence when the amino acid sequence is at least part of the final amino acid sequence of the protein. In such a fashion, the protein can be only the peptide or have additional amino acid molecules, such as amino acid residues (contiguous encoded sequence) that are naturally associated with it or heterologous amino acid residues/peptide sequences. Such a protein can have a few additional amino acid residues or can comprise several hundred or more additional amino acids. A
brief description of how various types of these proteins can be made/isolated is provided below.
The estrogen receptor protein of the present invention can be attached to heterologous sequences to form chimeric or fusion proteins. Such chimeric and fusion proteins comprise a estrogen receptor protein operatively linked to a heterologous protein having an amino acid sequence not substantially homologous to the estrogen receptor protein.
"Operatively linked"
indicates that the estrogen receptor protein and the heterologous protein are fused in-frame. The heterologous protein can be fused to the N-terminus or C-terminus of the estrogen receptor protein.
In some uses, the fusion protein does not affect the activity of the estrogen receptor protein per se. For example, the fusion protein can include, but is not limited to, enzymatic fusion proteins, for example beta-galactosidase fusions, yeast two-hybrid GAL
fusions, poly-His fusions, MYC-tagged, HI-tagged and Ig fusions. Such fusion proteins, particularly poly-His fusions, can facilitate the purification of recombinant estrogen receptor protein. In certain host cells (e.g., mammalian host cells), expression andlor secretion of a protein can be increased by using a heterologous signal sequence.
A chimeric or fusion protein can be produced by standard recombinant DNA
techniques.
For example, DNA fragments coding for the different protein sequences are ligated together in-frame in accordance with conventional techniques. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA
synthesizers.
Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and re-amplified to generate a chimeric gene sequence (see Ausubel et al., Curre~rt Protocols ih Molecular Biology, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST protein). A
estrogen receptor protein-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linleed in-frame to the estrogen receptor protein.
Polypeptides often contain amino acids other than the 20 amino acids commonly referred to as the 20 naturally-occurring amino acids. Further, many amino acids, including the terminal amino acids, may be modified by natural processes, such as processing and other post-translational modifications, or by chemical modification techniques well known in the art. Common modifications that occur naturally in polypeptides are described in basic texts, detailed monographs, and the research literature, and they are well known to those of skill in the art. Accordingly, the polypeptides also encompass derivatives or analogs in which a substituted amino acid residue is not one encoded by the genetic code, in which a substituent group is included, in which the mature polypeptide is fused with another compound, such as a compound to increase the half life of the polypeptide (for example, polyethylene glycol), or in which the additional amino acids are fused to the mature polypeptide, such as a leader or secretory sequence or a sequence for purification of the mature polypeptide or a pro-protein sequence.
Known modifications include, but are not limited to, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent crosslinks, formation of cystine, formation of pyroglutamate, formylation, gamma carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic I 5 processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA
mediated addition of amino acids to proteins such as arginylation, and ubiquitination.
Such modifications are well-known to those of skill in the art and have been described in great detail in the scientific literature. Several particularly common modifications, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, for instance, are described in most basic texts, such as Pr~oteius - St~uctu~e ahd Molecular Properties, 2nd Ed., T.E. Creighton, W. H.
Freeman and Company, New York (1993). Many detailed reviews are available on this subject, such as by Wold, F., Postt~anslatiohal Covalent Modification of Proteins, B.C. Johnson, Ed., Academic Press, New Yorlc 1-12 (1983); Seifter et al. (Meth. Enzymol. 182: 626-646 (1990)) and Rattan et al. (Av~~c. N. Y. Acad. Sci. 663:48-62 (1992)).
The present invention further provides fragments of the estrogen receptor proteins of the present invention, in addition to proteins and peptides that comprise and consist of such fragments. The fragments to which the invention pertains, however, are not to be construed as encompassing fragments that may be disclosed publicly prior to the present invention.
As used herein, a fragment comprises at least 8 or more contiguous amino acid residues from a estrogen receptor protein. Such fragments can be chosen based on the ability to retain one or more of the biological activities of the estrogen receptor protein or could be chosen for the ability to perform a function, e.g. act as an immunogen. Particularly important fragments are biologically active fragments, peptides which are, for example, about 8 or more amino acids in length, that contain a variant amino acid residue (Figure 2). Such fragments will typically comprise a domain or motif of the estrogen receptor proteins of the present invention, e.g., active site, ligand binding domain or DNA binding domain. Further, possible fragments include, but are not limited to, domain or motif containing fragments, soluble peptide fragments, and fragments containing immunogenic structures. Predicted domains and functional sites are readily identifiable by computer programs well-known and readily available to those of skill in the art (e.g., PROSITE analysis).
Protein/Peptide Uses The proteins of the present invention can be used in assays to determine the biological activity of the protein, including in a panel of multiple proteins for high-throughput screening; to raise antibodies or to,elicit another immune response; as a reagent (including the labeled reagent) in assays designed to quantitatively determine levels of the protein (or its binding partner or receptor) in biological fluids; and as maxkers for tissues in which the corresponding protein is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in a disease state). Any or all of these research utilities are capable of being developed into reagent grade or kit format for commercialization as research products. Methods for performing the uses listed above are well known to those skilled in the art. References disclosing such methods include "Molecular Cloning: A
Laboratory Manual", 2d ed., Cold Spring Harbor Laboratory Press, Sambrook, J., E. F.
Fritsch and T. Maniatis eds., 1989, and "Methods in Enzymology: Guide to Molecular Cloning Techniques", Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.
The estrogen receptor proteins of the present invention are useful for biological assay.
Such assays involve any of the known estrogen receptor functions or activities or properties useful for the diagnosis and treatment of estrogen receptor-related conditions.
The estrogen receptor proteins of the present invention are also useful in drug screening assays, in cell-based or cell-free systems. Cell-based systems can be native, i.e., cells that normally express the receptor protein, as a biopsy or expanded in cell culture. In one embodiment, however, cell-based assays involve recombinant host cells expressing the receptor protein.
The estrogen receptor proteins of the present invention can be used to identify compounds that modulate receptor activity. Both the estrogen receptor protein of the present invention and appropriate fragments can be used in high-throughput screens to assay candidate compounds for the ability to bind and/or modulate the activity of the receptor. These compounds can be further screened against a functional receptor to determine the effect of the compound on the receptor activity. Further, these compounds can be tested in animal or invertebrate systems to determine activity/effectiveness. Compounds can be identified that activate (agonist) or inactivate (antagonist) the receptor to a desired degree. Such compounds can be selected for the ability to act on one or more of the variant estrogen receptor proteins of the present invention.
Further, the receptor polypeptides can be used to screen a compound for the ability to stimulate or inhibit interaction between the receptor protein and a target molecule that normally interacts with the receptor protein, e.g. estrogen. The target can be ligand or a binding partner that the receptor protein normally interacts (for example, an estrogen ligand or a DNA
sequence). Such assays typically include the steps of combining the receptor protein with a candidate compound under conditions that allow the receptor protein, or fragment, to interact with the target molecule, and to detect the formation of a complex between the protein and the target or to detect the biochemical consequence of the interaction with the receptor protein and the target, such as any of the associated effects of DNA binding or signal transduction.
Candidate compounds include, for example, 1 ) peptides such as soluble peptides, including Ig-tailed fusion peptides and members of random peptide libraries (see, e.g., Lam et al., Nature 354:82-84 (1991); Houghten et al., Nature 354:84-86 (1991)) and combinatorial chemistry-derived molecular libraries made of D- and/or L- configuration amino acids; 2) phosphopeptides (e.g., members of random and partially degenerate, directed phosphopeptide libraries, see, e.g., Songyang et al., Cell 72:767-778 (1993)); 3) antibodies (e.g., polyclonal, monoclonal, humanized, anti-idiotypic, chimeric, and single chain antibodies as well as Fab, F(ab')2, Fab expression library fragments, and epitope-binding fragments of antibodies); and 4) small organic and inorganic molecules (e.g., molecules obtained from combinatorial and natural product libraries). .
One candidate compound is a soluble fragment of the receptor that competes for ligand binding. Other candidate compounds include mutant receptors or appropriate fragments containing mutations that affect receptor function and thus compete for ligand. Accordingly, a fragment that competes for ligand, for example with a higher affinity, or a fragment that binds ligand but does not allow release, is encompassed by the invention.

The invention further includes other end point assays to identify compounds that modulate (stimulate or inhibit) receptor activity. The assays typically involve an assay of events in the signal transduction pathway that indicate receptor activity. Thus, the expression of genes that are up- or down-regulated in response to the receptor protein dependent signal cascade can be assayed. In one embodiment, the regulatory region of such genes can be operably linked to a marker that is easily detectable, such as luciferase. Alternatively, phosphorylation of the receptor protein, or a receptor protein target, could also be measured. Any of the biological or biochemical functions mediated by the receptor can be used as an endpoint assay. These include all of the biochemical or biochemical/biological events described herein, in the references cited herein, incorporated by reference for these endpoint assay targets, and other functions known to those of ordinary skill in the art.
The receptor polypeptides are also useful in competition binding assays in methods designed to discover compounds that interact with the receptor. Thus, a compound is exposed to a receptor polypeptide under conditions that allow the compound to bind or to otherwise interact with the polypeptide. Ligands to the receptor is also added to the mixture. If the test compound interacts with the receptor or ligand, it decreases the amount of complex formed or activity from the receptor target. This type of assay is particularly useful in cases in which compounds are sought that interact with specific regions of the receptor.
To perform cell free drug screening assays, it is sometimes desirable to immobilize either the receptor protein, or fragment, or its target molecule to facilitate separation of complexes from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay.
Techniques for immobilizing proteins on matrices can be used in the drug screening assays. In one embodiment, a fusion protein can be provided which adds a domain that allows the protein to be bound to a matrix. For example, glutathione-S-transferase/15625 fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St.
Louis, MO) or glutathione derivatized microtitre plates, which are then combined with the cell lysates (e.g., 35S-labeled) and the candidate compound, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH).
Following incubation, the beads are washed to remove any unbound label, and the matrix immobilized and radiolabel determined directly, or in the supernatant after the complexes are dissociated. Alternatively, the complexes can be dissociated from the matrix, separated by SDS-PAGE, and the level of receptor-binding protein found in the bead fraction quantitated from the gel using standard electrophoretic techniques. For example, either the polypeptide or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin using techniques well known in the art. Alternatively, antibodies reactive with the protein but which do not interfere with binding of the protein to its target molecule can be derivatized to the wells of the plate, and the protein trapped in the wells by antibody conjugation. Preparations of a receptor-binding protein and a candidate compound are incubated in the receptor protein-presenting wells and the amount of complex trapped in the well can be quantitated. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the receptor protein target molecule, or which are reactive with receptor protein and compete with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.
Agents that modulate the protein of the present invention can be identified using one or more of the above assays, alone or in combination. It is generally preferable to use a cell-based or cell free system first and then confirm activity in an animal or other model system. Such model systems are well known in the art and can readily be employed in this context.
Modulators of receptor protein activity identified according to these drug-screening assays can be used to treat a subject with a disorder mediated by the receptor pathway, by treating cells that express the estrogen receptor protein. These methods of treatment include the steps of administering the modulators of protein activity in a pharmaceutical composition as described herein, to a subject in need of such treatment.
This invention further pertains to novel agents identified by the above-described screening assays. Accordingly, it is within the scope of this invention to further use an agent identified as described herein in an appropriate animal model. For example, an agent identified as described herein (e.g., an estrogen receptor modulating agent, an antisense estrogen receptor nucleic acid molecule, an estrogen receptor-specific antibody, or an estrogen receptor-binding partner) can be used in an animal model to determine the efficacy, toxicity, or side effects of treatment with such an agent. Alternatively, an agent identified as described herein can be used in an anirrial model to determine the mechanism of action of such an agent. Furthermore, this invention pertains to uses of novel agents identified by the above-described screening assays for treatments as described herein.
The estrogen receptor proteins of the pr esent invention are also useful to provide a target for diagnosing a disease or predisposition to disease mediated by the estrogen receptor.

Accordingly, the invention provides methods for detecting the presence, or levels of, the estrogen receptor variants of the present invention (or encoding mRNA) in a cell, tissue, or organism. The method involves contacting a biological sample with a compound capable of interacting with the receptor protein (or gene or mRNA encoding the receptor) such that the interaction can be detected.
One agent for detecting a protein in a sample is an antibody capable of selectively binding to a variant form of the estrogen receptor protein. Such samples include tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject.
The estrogen receptor proteins of the present invention also provide targets for diagnosing active disease, or predisposition to disease, in a patient having a variant estrogen receptor, particularly a disease involving the estrogen pathway, such as bone growth, cell differentiation, etc. Thus, the receptor can be isolated from a biological sample and assayed for the presence of a genetic mutation that results in aberrant receptor activity.
This includes amino acid substitution, deletion, insertion, rearrangement, (as the result of aberrant splicing events), and inappropriate post-translational modification as provided in Figure 2.
Analytic methods include altered electrophoretic mobility, altered tryptic peptide digest, altered receptor activity in cell-based or cell-free assay, alteration in ligand or antibody-binding pattern, altered isoelectric point, direct amino acid sequencing, and any other of the known assay techniques useful for detecting mutations in a protein. Particularly useful are the variation provided in Figure 2.
In vitro techniques for detection of peptide include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence.
Alternatively, the peptide can be detected in vivo in a subject by introducing into the subject a labeled anti-peptide antibody. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. Particularly useful are methods that detect the specific allelic variants of the estrogen receptor disclosed herein that are expressed in a subject and methods that detect fragments of a peptide in a sample.
The peptides are also useful in pharmacogenomic analysis. Pharmacogenomics deal with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See, e.g., Eichelbaum, M.
(Clip. Exp.
Pharw2acol. Physiol. 23(I0-11) :983-985 (1996)), and Linder, M.W. (Clip.
Chena. 43(2):254-266 (1997)). The clinical outcomes of these variations result in severe toxicity of therapeutic drugs in certain individuals or therapeutic failure of drugs in certain individuals as a result of individual variation in metabolism. Thus, the genotype of the individual can determine the way a therapeutic compound acts on the body or the way the body metabolizes the compound.
Further, the activity of drug metabolizing enzymes effects both the intensity and duration of drug action. Thus, the pharmacogenomics of the individual permit the selection of effective S compounds and effective dosages of such compounds for prophylactic or therapeutic treatment based on the individual's genotype. The discovery of genetic polymorphisms in some drug metabolizing enzymes has explained why some patients do not obtain the expected drug effects, show an exaggerated drug effect, or experience serious toxicity from standard drug dosages.
Polymorphisms can be expressed in the phenotype ofthe extensive metabolizer and the phenotype of the poor metabolizer. Accordingly, genetic polymorphism may lead to allelic protein variants of the receptor protein in which one or more of the receptor functions in one population is different from those in another population. The peptides thus allow a target to ascertain a genetic predisposition that can affect treatment modality. Thus, in a ligand-based treatment, polymorphism may give rise to amino terminal extracellular domains and/or other I 5 Iigand-binding regions that are more or less active in ligand binding, and receptor activation.
Accordingly, ligand dosage would necessarily be modified to maximize the therapeutic effect within a given population containing a polymorphism/haplotype. As an alternative to genotyping, specific polymorphic peptides could be identified.
Antibodies The invention also provides antibodies that selectively bind to the estrogen receptor proteins of the present invention as well as fragments thereof. As used herein, an antibody selectively binds a target protein when it binds the target protein and does not significantly bind to unrelated proteins. An antibody is still considered to selectively bind a protein even if it also binds to other proteins that are not substantially homologous with the target protein so long as such proteins share homology with a fragment or domain of the protein target of the antibody.
In tlus case, it would be understood that antibody binding to the protein is still selective despite some degree of cross-reactivity.
As used herein, an antibody is defined in terms consistent with that recoguzed within 3 0 the art: they are multi-subunit proteins produced by a mammalian organism in response to an antigen challenge. The antibodies of the present invention include polyclonal antibodies and monoclonal antibodies, as well as fragments of such antibodies, including, but not limited to, Fab or F(ab')2, and Fv fragments.

Many methods are known for generating and/or identifying antibodies to a given target peptide. Several such methods are described by Harlow, Antibodies, Cold Spring Harbor Press, (1989). In general, to generate antibodies, an isolated peptide is used as an immunogen and is administered to a mammalian organism, such as a rat, rabbit or mouse. The full-length protein, an antigenic peptide fragment or a fusion protein can be used.
Antibodies are preferably prepared from regions or discrete fragments of the estrogen receptor protein. Antibodies can be prepared from any region of the peptide as described herein. However, preferred regions will include those involved in function/activity and/or receptor/binding partner interaction. An antigenic fragment will typically comprise at least 10 contiguous amino acid residues. The antigenic peptide can comprise, however, at least 12, 14, or more amino acid residues. Such fragments can be selected on a physical property, such as fragments correspond to regions that are located on the surface of the protein, e.g., hydrophilic regions or can be selected based on sequence uniqueness.
Detection on an antibody of the present invention can be facilitated by coupling (i.e., 15 physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, (3-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of 20 suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include lash i3ih ass or 3H.
Antibod, The antibodies can be used to isolate the estrogen receptor protein of the present invention by standard techniques, such as affinity chromatography or immunoprecipitation. The antibodies can facilitate the purification of the natural protein from cells and recombinantly 3 0 produced protein expressed in host cells. In addition, such antibodies are useful to detect the presence of the estrogen receptor protein of the present invention in cells or tissues to determine the pattern of expression of the protein among various tissues in an organism and over the course of normal development. Further, such antibodies can be used to detect protein in situ, in vitro, or in a cell lysate or supernatant in order to evaluate the abundance and pattern of expression.
Also, such antibodies can be used to assess abnormal tissue distribution or abnormal expression during development. Antibody detection of circulating fragments of the full length estrogen receptor protein can be used to identify turnover.
Further, the antibodies can be used to assess expression in disease states such as in active stages of the disease or in an individual with a predisposition toward disease related to the protein's function, particularly diseases involving bone growth/formation/degeneration. When a disorder is caused by an inappropriate tissue distribution, developmental expression, level of expression of the protein, or expressed/processed form, the antibody can be prepared against the normal protein. If a disorder is characterized by a specific mutation in the protein, antibodies specific for tlus mutant protein can be used to assay for the presence of the specific mutant protein.
The antibodies can also be used to assess normal and aberrant subcellular localization of cells in the various tissues in an organism. The diagnostic uses can be applied, not only in genetic testing, but also in monitoring a treatment modality. Accordingly, where treatment is ultimately aimed at correcting the expression level or the presence of aberrant sequence and aberrant tissue distribution or developmental expression, antibodies directed against the protein or relevant fragments can be used to monitor therapeutic efficacy.
Additionally, antibodies are useful in pharmacogenomic analysis. Thus, antibodies prepared against polymorphic proteins can be used to identify individuals that require modified treatment modalities. The antibodies are also useful as diagnostic tools as an immunological marker for aberrant estrogen receptor protein analyzed by electrophoretic mobility, isoelectric point, tryptic peptide digest, and other physical assays known to those in the art.
The antibodies are also useful for inhibiting protein function, for example, blocking the binding of the estrogen receptor protein to a binding partner such as a ligand. These uses can also be applied in a therapeutic context in which treatment involves inhibiting the protein's function. An antibody can be used, for example, to block binding, thus modulating (agonizing or antagonizing) the peptides activity. Antibodies can be prepared against specific fragments containing sites required for function or against intact protein that is associated with a cell or cell 3 0 membrane.
The invention also encompasses kits for using antibodies to detect the presence of a protein in a biological sample. The kit can comprise antibodies such as a labeled or labelable antibody and a compound or agent for detecting estrogen receptor protein in a biological sample;

means for determining the amount of protein in the sample; means for comparing the amount of estrogen receptor protein in the sample with a standard; and instructions for use.
Nucleic Acid Molecules The present invention fiu-ther provides isolated nucleic acid molecules that encode any of the estrogen receptor proteins of the present invention. Such nucleic acid molecules will consist of, consist essentially of, or comprise a nucleotide sequence that encodes one of the estrogen receptor proteins of the present invention.
As used herein, an "isolated" nucleic acid molecule is one that is separated from other nucleic acid present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. However, there can be some flanking nucleotide sequences, for example up to about SKB, 4KB, 3KB, 2I~B, or 1KB or less, particularly contiguous peptide encoding sequences and peptide encoding sequences within the same gene but separated by introns in the genomic sequence. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences such that it can be subjected to the specific manipulations described herein such as recombinant expression, preparation of probes and primers, and other uses specific to the nucleic acid sequences.
Moreover, an "isolated" nucleic acid molecule, such as a cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated.
For example, recombinant DNA molecules contained in a vector are considered isolated.
Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA
molecules in solution.
Isolated RNA molecules include in vivo or i~ vitro RNA transcripts of the isolated DNA
molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.
Accordingly, the present invention provides nucleic acid molecules that consist of the nucleotide sequences shown in Figure l, including one or more of the sequence polymorphisms provided in Figure 2. A nucleic acid molecule consists of a nucleotide sequence when the nucleotide sequence is the complete nucleotide sequence of the nucleic acid molecule.
The present invention fiufiher provides nucleic acid molecules that consist essentially of the nucleotide sequence shown in Figure 1, including one or more of the sequence polymorplusms provided in Figure 2. A nucleic acid molecule consists essentially of a nucleotide sequence when such a nucleotide sequence is present with only a few additional nucleic acid residues in the final nucleic acid molecule.
The present invention fuxther provides nucleic acid molecules that are comprised of the nucleotide sequences shown in Figure 1, including one or more of the sequence polymorphisms provided in Figure 2. A nucleic acid molecule is comprised of a nucleotide sequence when the nucleotide sequence is at least part of the final nucleotide sequence of the nucleic acid molecule.
In such a fashion, the nucleic acid molecule can be only the nucleotide sequence or have .
additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences. Such a nucleic acid molecule can have a few additional nucleotides or can comprise several hundred or more additional nucleotides. A
brief description of how various types of these nucleic acid molecules can be readily made/isolated is provided below.
The isolated nucleic acid molecules can encode the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature peptide (when the mature form has more than one peptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, facilitate protein trafficking, prolong or shorten protein half life or facilitate manipulation of a protein for assay or production, among other things. As generally is the case ih situ, the additional amino acids may be processed away from the mature protein by cellular enzymes.
As mentioned above, the isolated nucleic acid molecules include, but are not limited to, the sequence encoding the estrogen receptor protein alone, the sequence encoding the mature peptide and additional coding sequences, such as a leader or secretory sequence (e.g., a pre-pro or pro-protein sequence), the sequence encoding the mature peptide, with or without the additional coding sequences, plus additional non-coding sequences, for example introns and 3 0 non-coding 5' and 3' sequences such as transcribed but non-translated sequences that play a role in transcription, mRNA processing (including splicing and polyadenylation signals), ribosome binding and stability of mRNA, as well as genomic regulatory sequences such as promoters. In addition, the nucleic acid molecule may be fused to a marker sequence encoding, for example, a peptide that facilitates purification.
Isolated nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the non-coding strand (anti-sense strand).
The invention further provides nucleic acid molecules that encode fragments of the proteins of the present invention. A fragment comprises a contiguous nucleotide sequence greater than 12 or more nucleotides. Further, a fragment could at least 30, 40, 50, 100, 250 or 500 nucleotides. in length. The length of the fragment will be based on its intended use. For example, the fragment can encode epitope-bearing regions of the peptide, or can be useful as DNA probes and primers. Such fragments can be isolated using the known nucleotide sequence to synthesize an oligonucleotide probe. A labeled probe can then be used to screen 1 S a cDNA library, genomic DNA library, or mRNA to isolate nucleic acid corresponding to the coding region. Further, primers can be used in PCR reactions to clone specific regions of gene.
A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides.
As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 50-55% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 65%, at least about 70%, or at least about 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols i~ Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45C, followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-65C.

Nucleic Acid Molecule Uses The nucleic acid molecules of the present invention are useful for probes, primers, chemical intermediates, and in biological assays. The probe can correspond to any sequence along the entire length of the nucleic acid molecules provided in Figure l, including one or more of the sequence polymorphisms provided in Figure 2. Accordingly, it could be derived from 5' noncoding regions, the coding region, and 3' noncoding regions. However, as discussed, fragments are not to be construed as encompassing fragments disclosed prior to the present invention.
The nucleic acid molecules are also useful as primers for PCR to amplify any given region of a nucleic acid molecule and are useful to synthesize antisense molecules of desired length and sequence.
The nucleic acid molecules are also useful for constructing recombinant vectors. Such vectors include expression vectors that express a portion of, or all of, the peptide sequences.
Vectors also include insertion vectors, used to integrate into another nucleic acid molecule sequence, such as into the cellular genome, to alter in situ expression of a gene and/or gene product. For example, an endogenous coding sequence can be replaced via homologous recombination with all or part of the coding region containing one or more specifically introduced mutations.
The nucleic acid molecules are also useful for expressing antigenic portions of the proteins.
The nucleic acid molecules are also useful for designing ribozymes corresponding to all, or a part, of the mRNA produced from the nucleic acid molecules described herein.
The nucleic acid molecules are also useful for constructing host cells expressing a part, or all, of the nucleic acid molecules and peptides.
The nucleic acid molecules are also useful for constructing transgenic animals expressing all, or a part, of the nucleic acid molecules and peptides.
The nucleic acid molecules are also useful for making vectors that express part, or all, of the peptides.
The nucleic acid molecules are also useful as hybridization probes for determining the presence, level, form and distribution of nucleic acid expression.
Accordingly, the probes can be used to detect the presence of, or to determine levels of, a specific nucleic acid molecule in cells, tissues, and in organisms. The nucleic acid whose level is determined can be DNA or RNA.
Accordingly, probes corresponding to the peptides described herein can be used to assess expression and/or, gene copy number in a given cell, tissue, or organism.
These uses are relevant for diagnosis of disorders involving an increase or decrease in estrogen receptor protein expression relative to normal results.
1h vitro techniques for detection of mRNA include Northern hybridizations and iu situ hybridizations. In vitro techniques for detecting DNA include Southern hybridizations and in situ hybridization.
Probes can be used as a part of a diagnostic test kit for identifying cells or tissues that express a estrogen receptor proteins of the present invention, such as by measuring a level of a receptor-encoding nucleic acid in a sample of cells from a subject e.g., mRNA
or genomic DNA, or determining if a receptor gene has been mutated.
Nucleic acid expression assays are useful for drug screening to identify compounds that modulate estrogen receptor nucleic acid expression.
The invention thus provides a method for identifying a compound that can be used to treat a disorder associated with nucleic acid expression of the estrogen receptor gene. The method typically includes assaying the ability of the compound to modulate the expression of the estrogen receptor nucleic acid and thus identifying a compound that can be used to treat a disorder characterized by undesired estrogen receptor nucleic acid expression.
The assays can be performed in cell-based and cell-free systems. Cell-based assays include cells naturally expressing the estrogen receptor nucleic acid or recombinant cells genetically engineered to express specific nucleic acid sequences.
The assay for estrogen receptor nucleic acid expression can involve direct assay of nucleic acid levels, such as mRNA levels, or on collateral compounds involved in the signal pathway. Further, the expression of genes that are up- or down-regulated in response to the estrogen receptor protein signal pathway can also be assayed. In this embodiment the regulatory regions of these genes can be operably linked to a reporter gene such as luciferase.
Thus, modulators of estrogen receptor gene expression can be identified in a method wherein a cell is contacted with a candidate compound and the expression of mRNA
determined. The level of expression of estrogen receptor mRNA in the presence of the candidate compound is compared to the level of expression of estrogen receptor mRNA in the 3 0 absence of the candidate compound. The candidate compound can then be identified as a modulator of nucleic acid expression based on this comparison and be used, for example to treat a disorder characterized by aberrant nucleic acid expression. When expression of mRNA is statistically significantly greater in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of nucleic acid expression. When nucleic acid expression is statistically significantly less in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of nucleic acid expression.
The invention fw-ther provides methods of treatment, with the nucleic acid as a target, using a compound identified through drug screening as a gene modulator to modulate estrogen receptor nucleic acid expression. Modulation includes both up-regulation (i.e.
activation or agonization) or down-regulation (suppression or antagonization) or nucleic acid expression.
Alternatively, a modulator for estrogen receptor nucleic acid expression can be a small molecule or drug identified using the screening assays described herein as long as the drug or small molecule inhibits the estrogen receptor nucleic acid expression.
The nucleic acid molecules are also useful for monitoring the effectiveness of modulating compounds on the expression or activity of the estrogen receptor gene in clinical trials or in a treatment regimen. Thus, the gene expression pattern can serve as a barometer for the continuing effectiveness of treatment with the compound, particularly with compounds to which a patient can develop resistance. The gene expression pattern can also serve as a marker indicative of a physiological response of the affected cells to the compound.
Accordingly, such monitoring would allow either increased administration of the compound or the administration of alternative compounds to which the patient has not become resistant.
Similarly, if the level of nucleic acid expression falls below a desirable level, administration of the compound could be commensurately decreased.
The nucleic acid molecules are also useful in diagnostic assays for qualitative changes in estrogen receptor nucleic acid, and particularly in qualitative changes that lead to pathology.
The nucleic acid molecules can be used to detect mutations in estrogen receptor genes and gene expression products such as mRNA. The nucleic acid molecules can be used as hybridization probes to detect naturally-occurring genetic mutations in the estrogen receptor gene and thereby to determine whether a subject with the mutation is at risk for a disorder caused by the mutation.
Mutations include deletion, addition, or substitution of one or more nucleotides in the gene, chromosomal rearrangement, such as inversion or transposition, modification of genomic DNA, such as aberrant methylation patterns or changes in gene copy number, such as amplification.
3 0 Detection of a mutated form of the estrogen receptor gene associated with a dysfunction provides a diagnostic tool for an active disease or susceptibility to disease when the disease results from overexpression, underexpression, or altered expression of a estrogen receptor protein.

Individuals carrying mutations in the estrogen receptor gene can be detected at the nucleic acid level by a variety of techniques. Genomic DNA can be analyzed directly or can be amplified by using PCR prior to analysis. RNA or cDNA can be used in the same way. In some uses, detection of the mutation involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Patent Nos. 4,683,195 and 4,683,202), such as anchor PCR
or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran et al., Science 241:1077-1080 (1988); and Nakazawa et al., PNAS 91:360-364 (1994)), the latter of which can be particularly useful for detecting point mutations in the gene (see Abravaya et al., Nucleic Acids Res. 23:675-682 (1995)). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a gene under conditions such that hybridization and amplification of the gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. Deletions and insertions can be detected by a change in size of the amplified product compared to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to normal RNA or antisense DNA sequences.
Alternatively, mutations in a estrogen receptor gene can be directly identified, for example, by alterations in restriction enzyme digestion patterns determined by gel electrophoresis.
Further, sequence-specific ribozymes (U.S.Patent No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site.
Perfectly matched sequences can be distinguished from mismatched sequences by nuclease cleavage digestion assays or by differences in melting temperature.
Sequence changes at specific locations can also be assessed by nuclease protection assays such as RNase and S 1 protection or the chemical cleavage method.
Furthermore, sequence differences between a mutant estrogen receptor gene and a wild-type gene can be determined by direct DNA sequencing. A variety of automated sequencing procedures can be utilized when performing the diagnostic assays ((1995) Biotechniques 19:448), including sequencing by mass spectrometry (see, e.g., PGT International Publication No.
WO 94/16101;
Cohen et al., Adv. Chy~onaatogy~. 36:127-162 (1996); and Griffin et al., Appl.
Biochem.
Biotechhol. 38:147-159 (1993)).

Other methods for detecting mutations in the gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA
duplexes (Myers et al., Science 230:1242 (1985)); Cotton et al., PNAS 85:4397 (1988);
Saleeba et al., Meth. Evczymol. 217:286-295 (1992)), electrophoretic mobility of mutant and wild type nucleic acid is compared (Orita et al., PNAS 86:2766 (1989); Cotton et al., Mutat.
Res. 285:125-144 (1993); and Hayashi et al., Genet. Anal. Tech. Appl. 9:73-79 (1992)), and movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (Myers et al., Nature 313:495 (1985)). Examples of other techniques for detecting point mutations include, selective oligonucleotide hybridization, selective amplification, and selective primer extension.
The nucleic acid molecules are also useful for testing an individual for a genotype that while not necessarily causing diseases; nevertheless affects the treatment modality. Thus, the nucleic acid molecules can be used to study the relationship between an individual's genotype and the individual's response to a compound used for treatment (pharmacogenomic relationship).
Accordingly, the nucleic acid molecules described herein can be used to assess the mutation content of the estrogen receptor gene in an individual in order to select an appropriate compound or dosage regimen for treatment.
Thus nucleic acid molecules displaying genetic variations that affect treatment provide a diagnostic target that can be used to tailor treatment in an individual.
Accordingly, the production of recombinant cells and animals containing these polymorphism/haplotypes allow effective clinical design of treatment compounds and dosage regimens.
The nucleic acid molecules are thus useful as antisense constructs to control estrogen receptor gene expression in cells, tissues, and organisms. A DNA antisense nucleic acid molecule is designed to be complementary to a region of the gene involved in transcription, preventing transcription and hence production of estrogen receptor protein. An antisense RNA
or DNA nucleic acid molecule would hybridize to the mRNA and thus block translation of mRNA into estrogen receptor protein.
Alternatively, a class of antisense molecules can be used to inactivate mRNA
in order to decrease expression of estrogen receptor nucleic acid. Accordingly, these molecules can treat a disorder characterized by abnormal or undesired estrogen receptor nucleic acid expression. This technique involves cleavage by means of ribozymes containing nucleotide sequences complementary to one or more regions in the mRNA that attenuate the ability of the mRNA to be translated. Possible regions include coding regions and particularly coding regions corresponding to the catalytic and other functional activities of the estrogen receptor proteins of the present invention, such as ligand binding.
The nucleic acid molecules also provide vectors for gene therapy in patients containing cells that are aberrant in estrogen receptor gene expression. Thus, recombinant cells, which include the patient's cells that have been engineered ex vivo and returned to the patient, are introduced into an individual where the cells produce the desired estrogen receptor protein to treat the individual.
The invention also encompasses kits for~detecting the presence of a estrogen receptor nucleic acid in a biological sample. For example, the kit can comprise reagents such as a labeled or labelable nucleic acid or agent capable of detecting estrogen receptor nucleic acid in a biological sample; means for determining the amount of estrogen receptor nucleic acid in the sample; and means for comparing the amount of estrogen receptor nucleic acid in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can fzxrther comprise instructions for using the kit to detect estrogen receptor protein mRNA or I S DNA.
Design of SNP-Containing Nucleic Acids Detection Methods The SNP-containing nucleic acid molecules of the present invention are useful as probes, primers, chemical intermediates, and in biological assays for SNPs of the present invention. The probes/primers can correspond to one or more of the SNPs provided in Figure 2 or can correspond to a specific region 5' and/or 3' to a SNP position. However, as discussed above, fragments are not to be construed as encompassing fragments that are not associated with SNPs of the present invention or those known in the art for SNP detection. The SNP-containing nucleic acid molecules and information provided herein are also useful for designing primers for PCR to amplify any given SNP of the present invention and to design any formatted SNP
detection reagent/kits.
A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides. Depending on the particular application, the consecutive nucleotides can either include the target SNP position, or be a specific region in close enough proximity 5' and/or 3' to the SNP position to carry out the desired assay.
Preferred primer and probe sequences can readily be determined using the sequences provided in Figures l, 2, and 9. It will be apparent to one of skill in the art that such primers and probes are useful as diagnostic probes or amplification primers for genotyping SNPs of the present invention, and can be incorporated into a kit format.
For analyzing SNPs, it may be appropriate to use oligonucleotides specific to alternative SNP alleles (referred to as "allele-specific oligonucleotides", "allele-specific probes", or "allele-s specific primers"). The design and use of allele-specific probes for analyzing polymorphisms is described by e.g., Saiki et al., Nature 324, 163-166 (1986); Dattagupta, EP
235,726, Saiki, WO 89/11548.
In a hybridization-based assay, allele-specific probes can be designed that hybridize to a segment of target DNA from one individual but do not hybridize to the corresponding segment from another individual due to the presence of different polymorphic forms in the respective segments from the two individuals. Hybridization conditions should be sufficiently stringent that there is a significant difference in hybridization intensity between alleles, and preferably an essentially binary response, whereby a probe hybridizes to only one of the alleles. Some probes are designed to hybridize to a segment of target DNA such that the polymorphic site aligns with a central position (e.g., in a 15-mer at the 7 position; in a 16-mer, at either the 8 or 9 position) of the probe. This design of probe achieves good discrimination in hybridization between different allelic forms.
Allele-specific probes are often used in pairs, the "pairs" may be identical except for a one nucleotide mismatch that represents the allelic variants at the SNP
position. One member of a pair perfectly matches a reference form of a target sequence and the other member perfectly matches a variant form. In the case of an array, several pairs of probes can then be immobilized on the same support for simultaneous analysis of multiple polymorphisms within the same target sequence.
In one type of PCR-based assay, an allele-specific primer hybridizes to a site on target DNA overlapping the SNP position and only primes amplification of an allelic form to which the primer exhibits perfect complementarity. See Gibbs, Nucleic Acid Res. 17 (1989). This primer is used in conjunction with a second primer that hybridizes at a distal site. Amplification proceeds from the two-primers, resulting in a detectable product that indicates the particular allelic form is present. A control is usually performed with a second pair of primers, one of which shows a single base mismatch at the polymorphic site and the other of which exhibits perfect complementarity to a distal site. The single-base mismatch prevents amplification and no detectable product is formed. The method works best when the mismatch is included in the 3'-most position of the oligonucleotide aligned with the polymorphism because this position is most destabilizing to elongation from the primer (see, e.g., WO 93/22456). This PCR-based assay can be utilized as part of the TaqMan assay, described below.
SNP Detection Kits, Nucleic Acid Arrays, and Integrated Systems The present invention further provides SNP detection kits, such as arrays or microarrays of nucleic acid molecules, or probe/primer sets, that are based on the SNPs provided in Figures l, 2, 4, 8, 9 In one embodiment of the present invention, kits are provided which contain the necessary reagents to carry out one or more assays that detect one or more SNPs disclosed herein. The present invention also provides multicomponent integrated systems for analyzing the SNPs provided by the present invention.
SNP detection kits may contain one or more oligonucleotide probes, or pairs of probes, that hybridize at or near each SNP position. Multiple pairs of allele-specific oligonucleotides may be included in the kit to simultaneously assay large numbers of SNPs, at least one of which is one of the SNPs of the present invention. In some kits, such as arrays, the allele-specific oligonucleotides are provided immobilized to a substrate. For example, the same substrate can comprise allele-specific oligonucleotide probes for detecting at least l; 10; 100; 1000; 10,000; 100,000; 300,000 or substantially all of the polymorphisms shown in Figures l, 2, 4, 8 and 9.
Specifically, the invention provides a compartmentalized kit to receive, in close confinement, one or more containers which comprises: (a) a first container comprising one of the nucleic acid probes, for example an allele-specific oligonucleotide, that can bind to a fragment of the human genome containing a SNP disclosed herein; and (b) one or more other containers comprising one or more of the following: wash reagents or reagents capable of detecting the presence of a bound probe.
Tn detail, a compartmentalized kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers, strips of plastic, glass or paper, or arraying material such as silica. Such containers allow one to 3 0 efficiently transfer reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated, and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers may include a container which will accept the test sample, a container which contains the SNP probe, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, etc.), and containers which contain the reagents used to detect the bound probe. The kit can further comprise reagents for PCR or other enzymatic reactions, and instructions for using the kit. One skilled in the art will readily recognize that the previously unidentified SNPs of the present invention can be routinely identified using the sequence information disclosed herein and can be readily incorporated into one of the established kit formats which are well known in the art.
The present invention further provides arrays or microarrays of nucleic acid molecules that are based on the sequence information provided in Figures l, including one or more of the variations provided in Figure 2.
As used herein "Arrays" or "Microarrays" refers to an array of distinct polynucleotides or oligonucleotides synthesized on a substrate, such as paper, nylon or other type of membrane, filter, chip, glass slide, or any other suitable solid support. In one embodiment, the microarray is prepared and used according to the methods described in US
Patent 5,837,832, Chee et al., PCT application W095/11995 (Chee et al.), Lockhart, D. J. et al. (1996; Nat. Biotech. 14: 1675-1680) and Schena, M. et al. (1996; Proc.
Natl. Acad. Sci.
93: 10614-10619), all of which are incorporated herein in their entirety by reference. In other embodiments, such arrays axe produced by the methods described by Brown et al., US Patent No. 5,807,522. Arrays or microarrays are commonly referred to as "DNA chips".
Any number of oligonucleotide probes, such as allele-specific oligonucleotides, may be implemented in an array, wherein each probe or pair of probes corresponds to a different SNP
position. The oligonucleotides are synthesized at designated areas on a substrate using a light-directed chemical process. The substrate may be paper, nylon or other type of membrane, filter, chip, glass slide or any other suitable solid support.
Hybridization assays based on oligonucleotide arrays rely on the differences in hybridization stability of short oligonucleotides probes to perfectly matched and mismatched target sequence variants. Efficient access to polymorphism information is obtained through a basic structure comprising high-density arrays of oligonucleotide probes attached to a solid support (e.g., a chip) at selected positions. Each DNA chip can contain thousands to millions 3 0 of individual synthetic DNA probes arranged in a grid-like pattern and miniaturized to the size of a dime, each corresponding to a particular SNP position or allelic variant. Preferably, probes are attached to a solid support in an ordered, addressable array.

The array/chip technology has already been applied with success in numerous cases.
For example, the screening of mutations has been undertaken in the BRCA1 gene, in S.
cerevisiae mutant strains, and in the protease gene of HIV- I virus (Hacia et al., 1996;
Shoemaker et al., 1996 ; I~ozal et al., 1996). Chips of various formats for use in detecting SNPs can be produced on a customized basis.
An array-based tiling strategy useful for detecting SNPs is described in EP
785280.
Briefly, arrays may generally be "tiled" for a large number of specific polymorphisms.
"Tiling" refers to the synthesis of a defined set of oligonucleotide probes that are made up of a sequence complementary to the target sequence of interest, as well as preselected variations of that sequence, e.g., substitution of one or more given positions with one or more members of the basis set of monomers, i.e. nucleotides. Tiling strategies are further described in PCT
application No. WO 95/11995. In a particular aspect, arrays are tiled for a number of specific SNPs. In particular, the array is tiled to include a number of detection blocks, each detection block being specific for a specific SNP or a set of SNPs. For example, a detection block may be tiled to include a number of probes that span the sequence segment that includes a specific SNP. To ensure probes that are complementary to each allele, the probes are synthesized in pairs differing at the SNP position. In addition to the probes differing at the SNP position, monosubstituted probes are also generally tiled within the detection block.
Such methods can readily be applied to the SNP information disclosed herein.
These monosubstituted probes have bases at and up to a certain number of bases in either direction from the polymorphism, substituted with the remaining nucleotides (selected from A, T, G, C and U). Typically the probes in a tiled detection block will include substitutions of the sequence positions up to and including those that are 5 bases away from the SNP. The monosubstituted probes provide internal controls for the tiled array, to distinguish actual hybridization from artefactual cross-hybridization. Upon completion of hybridization with the target sequence and washing of the array, the array is scanned to determine the position on the array to which the target sequence hybridizes.
The hybridization data from the scanned array is then analyzed to identify which allele or alleles of the SNP are present in the sample. Hybridization and scanning may be carried out as described in PCT application No. WO 92/10092 and WO 95/11995 and US patent No.
5,424,186.
Thus, in some embodiments, the chips may comprise an array of nucleic acid sequences of fragments of about 15 nucleotides in length. In further embodiments, the chip may comprise an array including at least one of the sequences selected from the group consisting of those disclosed in the Figuresl, 2, 8, 9, and the sequences complementary thereto, or a fragment thereof, said fragment comprising at least about 8 consecutive nucleotides, preferably 10, 15, 20, more preferably 25, 30, 40, 47, or 50 consecutive nucleotides and containing a polymorphic base. In some embodiments the polymorphic base is within 5, 4, 3, 2, or 1 nucleotides from the center of the polynucleotide, more preferably at the center of said polynucleotide. In other embodiments, the chip may comprise an array containing any number of polynucleotides of the present invention.
An oligonucleotide may be synthesized on the surface of the substrate by using a chemical coupling procedure and an ink jet application apparatus, as described in PCT
application W095/251116 (Baldeschweiler et al.) which is incorporated herein in its entirety by reference. In another aspect, a "gridded" array analogous to a dot (or slot) blot may be used to arrange and link cDNA fragments or oligonucleotides to the surface of a substrate using a vacuum system, thermal, UV, mechanical or chemical bonding procedures. An array, such as those described above, may be produced by hand or by using available devices (slot blot or dot blot apparatus), materials (any suitable solid support), and machines (including robotic instruments), and may contain 8, 24, 96, 384, 1536, 6144 or more oligonucleotides, or any other number which lends itself to the efficient use of commercially available instrumentation.
Using such arrays, the present invention provides methods of identifying the SNPs of the present invention in a sample. Such methods comprise incubating a test sample with an array comprising one or more oligonucleotide probes corresponding to at least one SNP position of the present invention, and assaying for binding of a nucleic acid from the test sample with one or more of the oligonucleotide probes. Such assays will typically involve arrays comprising oligonucleotides probes corresponding to many SNP positions and/or allelic variants of those SNP positions, at least one of which is a SNP of the present invention.
Conditions for incubating a nucleic acid molecule with a test sample vary.
Incubation conditions depend on the format employed in the assay, the detection methods employed, and the type and nature of the nucleic acid molecule used in the assay. One skilled in the art will recognize that any one of the commonly available hybridization, amplification or array assay 3 0 formats can readily be adapted to employ the novel SNPs disclosed herein.
Examples of such assays can be found in Chard, T, An Introduction to Radioimmunoassay and Related Techniques, Elsevier Science Publishers, Amsterdam, The Netherlands (1986);
Bullock, G. R.
et al., Techniques in hnmunocytochemistry, Academic Press, Orlando, FL Vol. 1 (1 982), Vol. 2 (1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of Enzyme Immunoassays: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1985).
The test samples of the present invention include, but are not limited to, nucleic acid extracts, cells, and protein or membrane extracts from cells, which may be obtained from any bodily fluids (such as blood, urine, saliva, phlegm, gastric juices, etc.), cultured cells, biopsies, or other tissue preparations. The test sample used in the above-described methods will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods of preparing nucleic acid, protein, or cell extracts are well known in the art and can be readily be adapted in order to obtain a sample that is compatible with the system utilized.
Multicomponent integrated systems may also be used to analyze SNPs. Such systems miniaturize and compartmentalize processes such as PCR and capillary electrophoresis reactions in a single functional device. An example of such technique is disclosed in US
. patent 5,589,136, which describes the integration of PCR amplification and capillary electrophoresis in chips.
Integrated systems can be envisaged mainly when microfluidic systems are used These systems comprise a pattern of microchannels designed onto a glass, silicon, quartz, or plastic wafer included on a microchip. The movements of the samples are controlled by electric, electroosmotic or hydrostatic forces applied across different areas of the microchip to create functional microscopic valves and pumps with no moving parts.
Varying the voltage controls the liquid flow at intersections between the micro-machined channels and changes the liquid flow rate for pumping across different sections of the microchip.
For genotyping SNPs, the microfluidic system may integrate, for example, nucleic acid amplification, minisequencing primer extension, capillary electrophoresis, and a detection method such as laser induced fluorescence detection.
In a first step, the DNA samples are amplified, preferably by PCR. Then, the amplification products are subjected to automated minisequencing reactions using ddNTPs (specific fluorescence for each ddNTP) and the appropriate oligonucleotide minisequencing primers which hybridize just upstream of the targeted polymorphic base. Once the extension at the 3' end is completed, the primers are separated from the unincorporated fluorescent ddNTPs by capillary electrophoresis. The separation medium used in capillary electrophoresis can be, for example, polyacrylamide, polyethyleneglycol or dextran. The incorporated ddNTPs in the single nucleotide primer extension products are identified by laser-induced fluorescence detection. This microchip can be used to process at least 96 to 3~4 samples, or more, in parallel.
Vectors/host cells The invention also provides vectors containing the nucleic acid molecules described herein. The term "vector" refers to a vehicle, preferably a nucleic acid molecule, that can transport the nucleic acid molecules. When the vector is a nucleic acid molecule, the nucleic acid molecules are covalently linked to the vector nucleic acid. With this aspect of the invention, the vector includes a plasmid, single or double stranded phage, a single or double stranded RNA or DNA viral vector, or artificial chromosome, such as a BAC, PAC, YAC, OR
MAC.
A vector can be maintained in the host cell as an extrachromosomal element where it replicates and produces additional copies of the nucleic acid molecules.
Alternatively, the vector may integrate into the host cell genome and produce additional copies of the nucleic acid molecules when the host cell replicates.
The invention provides vectors for the maintenance (cloning vectors) or vectors for expression (expression vectors) of the nucleic acid molecules. The vectors can function in procaryotic or eukaryotic cells or in both (shuttle vectors).
Expression vectors contain cis-acting regulatory regions that are operably linked in the vector to the nucleic acid molecules such that transcription of the nucleic acid molecules is allowed in a host cell. The nucleic acid molecules can be introduced into the host cell with a separate nucleic acid molecule capable of affecting transcription. Thus, the second nucleic acid molecule may provide a trans-acting factor interacting with the cis-regulatory control region to allow transcription of the nucleic acid molecules from the vector.
Alternatively, a traps-acting factor may be supplied by the host cell. Finally, a traps-acting factor can be produced from the vector itself. It is understood, however, that in some embodiments, transcription and/or translation of the nucleic acid molecules can occur in a cell-free system.
The regulatory sequence to which the nucleic acid molecules described herein can be 3 0 operably linked include promoters for directing mRNA transcription. These include, but are not limited to, the left promoter from bacteriophage 7~, the lac, TRP, and TAC
promoters from E.
coli, the early and late promoters from SV40, the CMV immediate early promoter, the adenovirus early and late promoters, and retrovirus long-terminal repeats.

In addition to control regions that promote transcription, expression vectors may also include regions that modulate transcription, such as repressor binding sites and enhancers.
Examples include the SV40 enhancer, the cytomegalovirus immediate early enhancer, polyoma enhancer, adenovirus enhancers, and retrovirus LTR enhancers.
In addition to containing sites for transcription initiation and control, expression vectors can also contain sequences necessary for transcription termination and, in the transcribed region a ribosome binding site for translation. Other regulatory control elements for expression include initiation and termination codons as well as polyadenylation signals. The person of ordinary skill in the art would be aware of the numerous regulatory sequences that are useful in expression vectors. Such regulatory sequences are described, for example, in Sambrook et al., Molecular Cloning: A Labo~ato~y Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, (1989).
A variety of expression vectors can be used to express a nucleic acid molecule. Such vectors include chromosomal, episomal, and virus-derived vectors, for example vectors derived from bacterial plasmids, from bacteriophage, from yeast episomes, from yeast chromosomal elements, including yeast artificial chromosomes, from viruses such as baculoviruses, papovaviruses such as SV40, Vaccinia viruses, adenoviruses, poxviruses, pseudorabies viruses, and retroviruses. Vectors may also be derived from combinations of these sources such as those derived from plasmid and bacteriophage genetic elements, eg. cosmids and phagemids.
Appropriate cloning and expression vectors for prokaryotic and eukaryotic hosts are described in Sambrook et al., Moleculaf° Cloning: A Labo~ato~y Manual. 2nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, (1989).
The regulatory sequence may provide constitutive expression in one or more host cells (i.e. tissue specific) or may provide for inducible expression in one or more cell types such as by temperature, nutrient additive, or exogenous factor such as a hormone or other ligand. A variety of vectors providing for constitutive and inducible expression in prokaryotic and eulcaryotic hosts are well known to those of ordinary skill in the art.
The nucleic acid molecules can be inserted into the vector nucleic acid by well-known methodology. Generally, the DNA sequence that will ultimately be expressed is joined to an 3 0 expression vector by cleaving the DNA sequence and the expression vector with one or more restriction enzymes and then ligating the fragments together. Procedures for restriction enzyme digestion and ligation are well known to those of ordinary skill in the art.

The vector containing the appropriate nucleic acid molecule can be introduced into an appropriate host cell for propagation or expression using well-known techniques. Bacterial cells include, but are not limited to, E. colt, Streptomyces, and Salmorzella typhimur ium. Eukaryotic cells include, but are not limited to, yeast, insect cells such as Drosophila, animal cells such as COS and CHO cells, and plant cells.
As described herein, it may be desirable to express the peptide as a fusion protein.
Accordingly, the invention provides fusion vectors that allow for the production of the peptides.
Fusion vectors can increase the expression of a recombinant protein, increase the solubility of the recombinant protein, and aid in the purification of the protein by acting for example as a ligand for affinity purification. A proteolytic cleavage site may be introduced at the junction of the fusion moiety so that the desired peptide can ultimately be separated from the fusion moiety.
Proteolytic enzymes include, but are not limited to, factor Xa, thrombin, and enterokinase.
Typical fusion expression vectors include pGEX (Smith et al., Gehe 67:31-40 (1988)), pMAL
(New England Biolabs, Beverly, MA) and pRITS (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Examples of suitable inducible non-fusion E. colt expression vectors include pTrc (Amann et al., Gene 69:301-315 (1988)) and pET 1 1d (Studier et al., Gene Expression Technology: Methods iu E~zzymology 185:60-89 (1990)).
Recombinant protein expression can be maximized in a host bacteria by providing a genetic background wherein the host cell has an impaired capacity to proteolytically cleave the recombinant protein. (Gottesman, S.; Gene Expz°essio>z Techv~ology:
Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Alternatively, the sequence of the nucleic acid molecule of interest can be altered to provide preferential codon usage for a specific host cell, for example E. colt. (Wada et al., Nucleic Acids Res.
20:2111-2118 (1992)).
The nucleic acid molecules can also be expressed by expression vectors that are operative in yeast. Examples of vectors for expression in yeast e.g., S.
cerevisiae include pYepSecl (Baldari, et al., EMBO J. 6:229-234 (1987)), pMFa (T~urjan et al., Cell 30:933-943(1982)), pJRY88 (Schultz et al., Gevze 54:113-123 (1987)), and pYES2 (Invitrogen Corporation, San Diego, CA).
The nucleic acid molecules can also be expressed in insect cells using, for example, baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf 9 cells) include the pAc series (Smith et al., Mol. Cell Biol. 3:2156-2165 (1983)) and the pVL series (Lucklow et al., Virology 170:31-39 (1989)).

In certain embodiments of the invention, the nucleic acid molecules described herein are expressed in mammalian cells using mammalian expression vectors. Examples of mammalian expression vectors include pCDM8 (Seed, B. Nature 329:840(1987)) and pMT2PC
(Kaufman et al., EMBO J. 6:187-195 (1987)).
The expression vectors listed herein are provided by way of example only of the well-known vectors available to those of ordinary skill in the art that would be useful to express the nucleic acid molecules. The person of ordinary skill in the art would be aware of other vectors suitable for maintenance propagation or expression of the nucleic acid molecules described herein. These are found for example in Sambrook, J., Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
The invention also encompasses vectors in which the nucleic acid sequences described herein are cloned into the vector in reverse orientation, but operably linked to a regulatory sequence that permits transcription of antisense RNA. Thus, an antisense transcript can be produced to all, or to a portion, of the nucleic acid molecule sequences described herein, including both coding and non-coding regions. Expression of this antisense RNA
is subject to each of the parameters described above in relation to expression of the sense RNA (regulatory sequences, constitutive or inducible expression, tissue-specific expression).
The invention also relates to recombinant host cells containing the vectors described herein. Host cells therefore include prokaryotic cells, lower eukaryotic cells such as yeast, other eukaryotic cells such as insect cells, and higher eukaryotic cells such as mammalian cells.
The recombinant host cells are prepared by introducing the vector constructs described herein into the cells by techniques readily available to the person of ordinary skill in the art.
These include, but are not limited to, calcium phosphate transfection, DEAF-dextran-mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection, lipofection, and other techniques such as those found in Sambrook, et al.
(Molecular Cloning: A
Laboratory Manual. 2nd, ed.,' Cold Spring Harbor Labof°ato~y, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).
Host cells can contain more than one vector. Thus, different nucleotide sequences can be introduced on different vectors of the same cell. Similarly, the nucleic acid molecules can be introduced either alone or with other nucleic acid molecules that are not related to the nucleic acid molecules such as those providing traps-acting factors for expression vectors. When more than one vector is introduced into a cell, the vectors can be introduced independently, co-introduced or joined to the nucleic acid molecule vector.
In the case of bacteriophage and viral vectors, these can be introduced into cells as packaged or encapsulated virus by standard procedures for infection and transduction. Viral vectors can be replication-competent or replication-defective. In the case in which viral replication is defective, replication will occur in host cells providing functions that complement the defects.
Vectors generally include selectable markers that enable the selection of the subpopulation of cells that contain the recombinant vector constructs. The marker can be contained in the same vector that contains the nucleic acid molecules described herein or may be on a separate vector. Markers include tetracycline or ampicillin-resistance genes for prokaryotic host cells and dihydrofolate reductase or neomycin resistance for eukaryotic host cells.
However, any maxker that provides selection for a phenotypic trait will be effective.
While the mature proteins can be produced in bacteria, yeast, mammalian cells, and other cells under the control of the appropriate regulatory sequences, cell-free transcription and translation systems can also be used to produce these proteins using RNA
derived from the DNA constructs described herein.
Where secretion of the peptide is desired, which is difficult to achieve with multi-transmembrane domain containing proteins such as estrogen receptors, appropriate secretion signals are incorporated into the vector. The signal sequence can be endogenous to the peptides or heterologous to these peptides.
Where the peptide is not secreted into the medium, which is typically the case with estrogen receptors, the protein can be isolated from the host cell by standard disruption procedures, including freeze thaw, sonication, mechanical disruption, use of lysing agents and the like. The peptide can then be recovered and purified by well-known purification methods including ammonium sulfate precipitation, acid extraction, anion or cationic exchange chromatography, phosphocellulose chromatography, hydrophobic-interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography, or high performance liquid chromatography.
3 0 It is also understood that depending upon the host cell in recombinant production of the peptides described herein, the peptides can have various glycosylation patterns, depending upon the cell, or maybe non-glycosylated as when produced in bacteria. In addition, the peptides may include an initial modified methionine in some cases as a result of a host-mediated process.

Uses of vectors and host cells The recombinant host cells expressing the peptides described herein have a variety of uses. First, the cells are useful for producing a estrogen receptor protein or peptide that can be further purified to produce desired amounts of estrogen receptor protein or fragments. Thus, host cells containing expression vectors are useful for peptide production.
Host cells are also useful for conducting cell-based assays involving the estrogen receptor protein or estrogen receptor protein fragments, such as those described above as well as other formats known in the art. Thus, a recombinant host cell expressing a native estrogen receptor protein is useful for assaying compounds that stimulate or inhibit estrogen receptor protein function.
Host cells are also useful for identifying estrogen receptor protein mutants in which these functions are affected. If the mutants naturally occur and give rise to a pathology, host cells containing the mutations are useful to assay compounds that have a desired effect on the mutant estrogen receptor protein (for example, stimulating or inhibiting function) which may not be indicated by their effect on the native estrogen receptor protein.
Genetically engineered host cells can be further used to produce non-human transgenic animals. A transgenic animal is preferably a mammal, for example a rodent, such as a rat or mouse, in which one or more of the cells of the animal include a transgene. A
transgene is exogenous DNA which is integrated into the genome of a cell from which a transgenic animal develops and which remains in the genome of the mature animal in one or more cell types or tissues of the transgenic animal. These animals are useful for studying the function of a estrogen receptor protein and identifying and evaluating modulators of estrogen receptor protein activity.
Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, and amphibians.
A transgenic animal can be produced by introducing nucleic acid into the male pronuclei of a fertilized oocyte, e.g., by microinjection, retroviral infection, and allowing the oocyte to develop in a pseudopregnant female foster animal. Any of the estrogen receptor protein nucleotide sequences can be introduced as a transgene into the genome of a non-human animal, such as a mouse.
Any of the regulatory or other sequences useful in expression vectors can form part of the transgenic sequence. This includes intronic sequences and polyadenylation signals, if not already included. A tissue-specific regulatory sequences) can be operably linked to the transgene to direct expression of the estrogen receptor protein to particular cells.
Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and axe described, for S example, in U.S. Patent Nos. 4,736,866 and 4,870,009, both by Leder et al., U.S. Patent No.
4,873,191 by Wagner et al. and in Hogan, B., Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986). Similar methods are used for production of other transgenic animals. A transgenic founder animal can be identified based upon the presence of the transgene in its genome and/or expression of transgenic mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a transgene can further be bred to other transgenic animals carrying other transgenes. A transgenic animal also includes animals in which the entire animal or tissues in the animal have been produced using the homologously recombinant host cells described herein.
In another embodiment, transgenic non-human animals can be produced which contain selected systems that allow for regulated expression of the transgene. One example of such a system is the crelloxP recombinase system of bacteriophage P 1. For a description of the crelloxP recombinase system, see, e.g., Lakso et al. PNAS 89:6232-6236 (1992).
Another example of a recombinase system is the FLP recombinase system of S. cerevisiae (O'Gorman et al. Science 251:1351-1355 (1991). If a c~elloxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein is required. Such animals can be provided through the construction of "double" transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.
Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, I. et al. Nature 385:810-813 (1997) and PCT
International Publication Nos. WO 97/07668 and WO 97/07669. In brief, a cell, e.g., a somatic cell, from the transgenic animal can be isolated and induced to exit the growth cycle and enter Go phase. The quiescent cell can then be fused, e.g., through the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated.
The reconstructed oocyte is then cultured such that it develops to morula or blastocyst and then transferred to pseudopregnant female foster animal. The offspring born of this female foster animal will be a clone of the animal from which the cell, e.g., the somatic cell, is isolated.

Transgenic animals containing recombinant cells that express the peptides described herein are useful to conduct the assays described herein in an in vivo context. Accordingly, the various physiological factors that are present ih vivo and that could effect ligand binding, estrogen receptor protein activation, and signal transduction, may not be evident from ih vitwo cell-free or cell-based assays. Accordingly, it is useful to provide non-human transgenic animals to assay in vivo estrogen receptor protein function, including ligand interaction, the effect of specific mutant estrogen receptor protein on estrogen receptor protein function and ligand interaction, and the effect of chimeric estrogen receptor protein. It is also possible to assess the effect of null mutations, that is mutations that substantially or completely eliminate one or more estrogen receptor protein functions.
EXAMPLES:
1. SNP Identification and Characterization Individual exons of estrogen receptor alpha (ESRl) were PCR amplified using primers flanking each adjacent sequence of exon (exon/intron boundaries), and the sequence of amplified fragments was analyzed. As the PCR template, genomic DNA from Coriell Diversity Panels (10 individuals in each of 5 ethnic groups) (see Figure 2 (b)), and/or Liverpool clinical breast tumor and matching blood samples from 48 patients (from tissue obtained in Liverpool, England, see Figure 2 (a)) was used. PolyPhred version 2.0 (D. A.
Nickerson, S. Taylor, N. I~olker, Univ. of Washington, 1998) was run on the sequences (with default settings) to visualize potential heterozygotes. Tagged sites were examined for quality to verify polymorphisms.
36 SNPs with a frequency greater than 2 and a quality score greater than 20 were found with 13 being unique to the clinical samples. 15 of the SNPs showed at least one instance of a change in heterozygosity in the clinical samples, and 4 showed at least one instance of a loss in heterozygosity. For the analysis, PCR primers were used for SNP
identification and detection (see Figure 2 (e)) Additionally, the primer set in Figure 2(d) and M13 primers were used for overlapping PCR and clone sequencing.

Table 1. Summary of SNPs found in the clinical samples. All SNPs had a frequency greater than 2 and a quality score greater than 20 (see Figure 8).
Number of SNPs 39 Number in Liverpool 34 Number in Coriell 26 Number unique to Coriell 5 Number unique in Liverpool12 Table 2: Summary of changes in heterozygosity in clinical samples. SNPs had a frequency greater than two and a quality score greater than 20 (See Figure 8).
Number of Liverpool SNPs 15 With >1 Change in Heterozygosity Number of Liverpool SNPs 4 With >1 case of Loss of Heterozygosity Figure 2 (c) included analysis SNPs in Liverpool control population vs. blood or tumor populations. There were 23 sites that were typed in both cases and controls.
Figure 5 shows the domain structure of the ESR1 protein and the position of many of the SNPs disclosed herein. Figure 6 provides a graphical representation of the SNPs and frequency of occurance in the samples tested.
Figure 8 (a)(1) shows the SNPs and frequency of occurance in Coriell Samples wherein the samples are collected from Northern European, Chinese, Indo-Pakistani, Africa American and Southwestern Native American ethnic groups. In 3' flanking Exon 8, the positions of the SNPs are based on the sequence of AL078582 that is in Genbank. The result can be used for detection proposes among the specific ethnic groups. Figure 8 (a)(2) shows the genotyping of SNP on cite 167989 (intron 1D, #9) in the Coriell panel. This panel comprises Caucasians, wherein 51 are males and 49 are females. The result shows that the frequency of the minor allele is 9%.
Figure 8 (b)(1) shows the SNPs and frequence of occurance in Liverpool samples wherein the samples are selected from the groups of blood samples and breast cancer samples from Norther Europeans.

Figure 8 (b)(2) shows the Taq Man genotyping results for SNP cite 167, 989 (intron 1D, #9) in additional 60 Liverpool patients. The result shows that the frequency of the minor allele (G) was 14% in blood and 10% in tumors. This is similar to the SNP
genotyping in Coriell samples shown in Figure 8(b)(1), wherein the frequency at the minor allele was 16%
in blood and 17% in tumors. The Liverpool control population (95 cases) shows that the frequency at the minor allele was 10.5% (Figure 2(c)).
2. Haplotype Analysis The method developed for SNP discovery was designed to recover haplotype data.
SNPs could be associated into a specific haplotype. The sample cDNA was from a random population present in unknown proportions. SNPs coming from a specific clone were clustered and built into haplotyes.
Data consist of two sample types (Figure 4(a) and (b)). Liverpool samples axe from 48 patients, and each patient had a tumor and blood sample typed. Coriell samples were controls, but they were not matched controls. Rather they included a mix of Europeans, Chinese, Indo-Pakistani, and African Americans. 46 SNPs in ESR1 were scored in the Liverpool samples. The same 46 SNPs plus an additional 6 SNPs at 3' of the RSR1 gene were scored in the Coriell sample.
These data were subjected to an analysis to infer the most likely haplotype phase of the individuals. The results appear in Figure 4 (a) where each haplotype has a number and the number after the dash is a count (or frequency) of that haplotype.
Figure 4 (b) is the non-singleton haplotype data that were fitted to a neighbor joining tree. If a tree were cut at the arrow, the Glade including 3L, 10-4, ... 73L
would be partitioned from the rest of the tree, as "Glade X". The following table will illustrate a difference in the incidence of tumors in haplotypes on Glade X vs. the rest of the tree. The incidence of each haplotype was first counted by adding the numbers after the dashes, wherein L represented the tumorous Liverpool samples and the non-L represented Coriell controls.
Glade X Rest of tree 3 0 Tumor 49 64 Control 4 49 A Chi-square was calculated based on the 2 x 2 table as of 21.29, which, with one degree of freedom that has a probability less than 0.0001. Therefore, the Glade X of the ERl gene has a much greater chance of being associated with a tumor. This entire Glade is so rare elsewhere in the world. Even among Europeans, it was present only once out of haplotypes.
The sites that identified the Glade with a large frequency difference between Coriell controls, Liverpool controls, Liverpool tumor samples were:
5 ESRl-exon 1A 170487 ; 9 ESRl-intron 1D 167989 ;
11 ESRl-exon 1E 64331 ; 17 ESRl-exon Intrn 3 243187 ;
20 ESR1-exon 4 306382 ; 24 ESRl-exon Intrn 6 423220 ;
28 ESRl-exon Intrn 7 459832 ; 29 ESRl-intron 7 459913 ;
35 ESRl-exon 8 460929 ; 45 ESRl-exon 8 462949 3. Promoter region and SNP on Estrogen receptor 1 CAAT and TATA boxes are found in the first 200 b.p. of the sequence and a distance between them lead to believe that they might be functional as a basal promoter.
The distance between CAAT-TATA sites and actual identified start of transcription is about 300 b.p. (usually it is about 20-40 b.p.) and this region contains multiple TF sites such NFAT, NFKB, SPl-family, CEBP and EBOX, majority of them involved in a differential (tissue, cell type) regulation of expression.
Region with T (SNP) has interesting properties. Not only it has conservative (TC)6-repeat, but also (CA)6 on the 5-prime. A conservative motif for is CACAYTCTC
at the same region. There is no significant match from known TF sites to this region, and it is likely to be a novel TF site. Very close to T (SNP) is TF site called AHRARNT 02 for aryl hydrocaxbon/Arnt heterodimers. It is possible that CACAYTCTC site is either a binding point for the co-factor or help to properly position Arnt complex. It is also known that a single mutation in TF-binding site could decrease affinity of the protein binding several folds and as such may lead into a disease pathway.
In the present invention, the SNP occurs just l3bp upstream of exon 1 C in a short GA
repeat (GAGAGAGA). Among SNPs of interest in the Glades (Figure 4), three are silent mutations, one is in the 3' UTR, and the rest are in intron regions.
The important T to G SNP (#9) is in the site 167989 (Intron ID) of the promoter region (Figure 2 (a), (b), and (c)). Loss of a gene copy is associated with cancer risk. A
promoter mutation that decreases gene expression could cause a similar effect.

G- to T transversion is in an alternate promoter site. There is some effect on estrogen physiology, possible on overall levels of signaling at the nucleus. The predition would be that the "effect" of the relevant Glade (and cladistic haplytypes) (Figure 4) is to increase overall estrogen receptor sensitivity and responsiveness or possible to lead in the direction of S alternative growth processes that cycle in the direction of unregulated (non-physiologic) growth signals. Estrogen exposure plays a critical role in breast cancer causation in virtually every epidemiologic study.
In addition, the TF binding sites and SNPs were detected in the ESRl gene wherein 5 SNPs occured in transcription binding sites as shown below:
SNP position Gene structure Transcription factor 167989 intron 1D PAX- 3 64331 exon 1E CDP

423220 intron 6 MEF-2 423232 intron 6 MEF-2 423258 intron 6 SRF,AGL3 4. ESRl Genomic Sequencing:- The Complete Genomic Structure of Estrogen Receptor alpha Estrogen receptor (ER) is a member of the nuclear hormone receptor gene superfamily. This family of genes is characterized by a modular structure with three distinct domains: a variable (N)-terminal domain, a highly conserved DNA binding domain, and a conserved (C)-terminal domain (reviewed in 1, 2). Functionally, the (N)-terminus domain regulates transactivation, the DNA binding domain regulates dimerization and DNA binding, and the (C)-terminus domain regulates transactivation, dimerization, ligand binding, nuclear translocation, silencing, and Heat Shock Protein binding. It was shown that the functions of the individual domains of the nuclear hormone gene superfamily are independent of the receptor in which they are found, and that the domains retain their function even when placed into different heterologous proteins (3,4,5). The domain modularity in the nuclear hormone receptor gene superfamily exists because the major subfamilies of these genes evolved 3 0 through a simple gene duplication early in evolution (6). The nuclear hormone receptor gene family can be separated according to two different classification schemes, one based on hormone binding, the other based on dimerization and how the receptors bind to their respective DNA
response elements (for a review, see 2).

The cDNA for ERa was first cloned and sequenced from the MCF-7 breast cancer cell line and was found to have 27% identity and 41 % conservation to the v-erb-A gene (7).
ERa was mapped to chromosome 6q25. l using Fluorescence In Situ Hybridization (FISH) and chromosome banding (8). In 1996, a novel estrogen receptor (ER(3) was identified by degenerate PCR (9) and mapped to 14q22-24 by FISH (10). ERa and ER(3 were shown to have 96% sequence identity in the DNA binding domain, 58% identity in the ligand-binding domain, and low similarity in the 5' and 3' ends as well as in the hinge (domain D). A
variety of ERa and ER(3 variants have since been described, including single and multiple exon deletions, truncated transcripts, and transcripts containing insertions ( 11,12,13). These variants were isolated from a variety of sources, including normal tissues, tumor tissues and cell lines. The ER status of tumors in breast cancer patients has been used as an indicator of response to endocrine therapy (14,15), and many studies have examined the role of ER in breast cancer tumor progression, ER-negative status, and hormone antagonist resistance (for a complete review, see 16).
Because of the importance of the ER gene, we set about to clone it in its entirety and determine its complete structure. Initially, we used standard Bacterial Artificial Chromosome (BAC) sequencing to generate sequence information for the coding regions of the genes. As Celera's sequencing of the human genome progressed, the remaining regions of ER were filled in using Celera regional assemblies. A small region of less than 25 kb was filled in on ERa using a public BAC (A1353611.6, positions 1,497-25,941) Materials and Methods 1 ) BAC Screening Appropriate markers were designed for ERa and ER(3 exons and used to obtain commercially available BAC clones from Research Genetics (Huntsville, AL). A
number of positive BACs were selected and individual clones were re-screened for verification.
2) DNA Isolation and Library Preparation BAC DNA was isolated from verified clones using QIAGEN columns (QIAGEN, Inc., Valencia, CA) according to the manufacturer's specifications. Shotgun libraries were prepared following standard protocols (17). Briefly, isolated BAC DNA was sonicated, polished, and size fractionated. Size selected DNA fragments were then subcloned into pUCl9 using standard ligation techniques. Ligated DNA was transformed into Electrocompetent cells (Life Technologies, Rockville, MD) and grown overnight.
3) DNA Sequencing and Annotation Sequencing reactions were performed using Big Dye Terminator chemistry (Applied Biosystems, Foster City, CA) and run on an ABI PRISM 3700 DNA Analyzer (Applied Biosystems). Phred (18), Phrap and Consed (19) were used for base calling, assembly, and finishing, respectively. Exon locations were determined using Cross Match to compare the published gene sequences to the genomic contig.
Results 1. Estrogen Receptor a Alignment of the genomic sequence for ERa and published mRNA sequences for ERa show the gene consists of 14 exons and covers 446,296 by of genomic sequence (Figure 7, Table 3).
2. Estrogen Receptor (3 Alignment of the genomic sequence for ER(3 and published mRNA sequences for ER(3 show the gene consists of 17 exons and covers 253,748 by of genomic sequence (figure 2, table 1). By analysis with the Celera Genome Browser, we were able to identify a gene, human synaptic nuclei expressed gene 2 (syne-2, accession number NM 015180.1), that is completely contained within intron 9 of ER/3, on the opposite strand. Further analysis of the syne-2 gene showed it consists of 21 exons, and covers 51,471 by of genomic sequence.
Discussion Alignment of the complete ERa genomic sequence and various ER(3 transcripts shows that the gene covers 446,296 by of genomic sequence and consists of 14 exons. The alignment of the published sequence for exon 1E (AJ002561) (20) and the ER a genomic sequence revealed that exon 1 E actually consists of two separate exons. The newly delineated exon is referred to here as exon 1 G to conform to the naming convention previously established. Exon 1G is located approximately 45 kb upstream of exon 1E and conforms to the GT/AG splice site consensus sequence (Figure 1, table 1).
Alignment of the various ER(3 transcripts to the complete ER~3 genomic sequence reveals a more complex organization than was previously accepted (13). The 5' UTR of the ER(3cx variant (AB006589) actually consists of seven untranslated exons (referred to here as exons -1 through -7), all of which conform to the GT/AG splice site consensus sequence (figure 2, table 1). Sequence alignment ofER(3 variants AF061055 and AF061054 (12) showed that these transcripts both contain intron sequence and were probably partially mature transcripts. Both of these partially mature transcripts contain exon 7 and a portion of exon 9, but do not conform to the splice site consensus sequence at the sites where intron sequence is present.
By examining the ER genomic sequences using the Celera Genome Browser, we were able to identify a separate gene contained entirely within intron 9 of ER(3.
This gene was identified as human synaptic nuclei expressed gene 2 (syne-2) and was shown to cover over 50 Kb of genomic sequence and consist of 21 exons, all of which conform to the GT/AG
splice site consensus sequence (Table 4). The syne-2 gene is located on the antisense strand of ER(3.
Completion of the sequence and structures for ERa and ERj3 should contribute to further understanding and characterization of these important receptors.
Table 3: Exon-Intron Boundaries and Locations in the Human Estrogen Receptor:
Exon sequences are shown in upper case and intron sequences are shown in lower case. Splice sites are shown in bold.
Exon Contig Contig Exon Size """"
Gene no, Splice varient Start end 5' splice donor 3' splice acceptor b size p) (Kb) ER1 1G AJ0025611894119032- AcCAAAGAAGgtaagttttt91 33.79 1F AJ0025625281852940- TTCTCTTCAAgtaggtactc122 11.21 1E AJ0025616415064280aaaacaaaagGAAGAAGAAACATCACTGAGgtatgtgtga130 101.95 1D AJ002560166228166322- GAGAGAGCCAgtaagtcacg94 1.68 1C X62462 168002168120- ATCCAGCAGGgtaggcttgt118 1.55 1B AJ002559169674169825- GACAAGTAAAgtaaagttca151 0.04 1A X03635 169867170678- CATTCTACAGgtacccgcgc811 34.23 2 X03635 204912205102ttccccccagGCCAAATTCAAGTATTCAAGgtaatagtgt190 37.87 3 X03635 242970243086cttttaatagGACATAACGAATGAAAGGTGgtaggtacat116 63.08 4 X03635 306168306503gtgttttcagGGATACGAAAAGGGTGCCAGgtaagaatgc335 67.14 5 X03635 373640373778ttgttttcagGCTTTGTGGATCTTGGACAGgtaagtgacc138 49.19 6 X03635 422964423097gttttcatagGAACCAGGGACTTAATTCTGgtgagttgat133 33.26 7 X03635 456354456537gcgcattcagGAGTGTACACGGCACATGAGgtgaggcatc183 4.16 8 X03635 460701465237ccacctacagTAACAAAGGC- 4536 -ER2 -7 AB0065894955249750- GGTTCTGAAGgtgcgtggtt198 1.18 -6 AB0065895092851235tgcctcttagACATCCAAGTTGTTTGTAAGgtaataaaaa307 32.62 -5 AB0065898385884041tatccactagAGGGAGACATGAGAACACAGgtgaacttca183 1.90 -4 AB0065898594286154ctctccatagAAATCCTGGGATTAGCCCTGgtaaggagct212 2.88 -3 AB0065898903789130cattcaacagTATCTGGGCTGTGCAGGTAGgtaggtaaag93 0.67 -2 AB0065898980389988ccttttacagGGTTTTGTTTGTGTTGACAGgtaagatgag185 3.12 -1 AF0605559311193488- TATCTGCAAGgtaagcgccc377 10.96 1 AF060555104446104897ttctttacagCCATTATACTCTGTAAACAGgtaagtccag451 2.47 2 AF060555107368107540tgctccctagAGAGACACTGAGCATTCAAGgtacaagaga172 11.07 3 AF060555118610118726tctgctatagGACATAATGAGTGAAGTGTGgtgagtgctt116 8.05 4 AF060555126774127073tcctcttcagGCTCCCGGAGAAGATTCCCGgtagggcttt299 3.09 5 AF060555130158130296ctttccccagGCTTTGTGGATTCTGGACAGgtgagaaaaa138 7.56 6 AF060555137853137986acttttgtagGGATGAGGGGCTCAATTCCAgtaagtaatc133 14.39 7 AF060555152379152559ctttgtccagGTATGTACCCGGCATGCGAGgtacgcgccc180 1.65 8 X99101 154206154500gtccccatagTAACAAGGGC- 826 5.42 9 AB006589159915160827tctacttaagGGCAGAAAAG- 912 141.65 10 AF060555302474303300gtcttgacagCTCTCTCTCA- 826 -IS

Table 4: Exon-Intron Boundaries and Locations in the Human Synaptic Nuclei Expressed Gene 2. Exon sequences are shown in upper case and intron sequences are shown in lower case. Splice sites are shown in bold.
Exon Splice Contig Contig Exon "'n "
Gene no. varient start end 5~ splice donor 3' splice acceptor Size size (bP) ~Kw Syne-21 NM_015180212563212391- CACTGTAGAGgtaaactcac172 2.22 2 NM 210175210044tttcaaatagACCTGGGACCGCTGATTAAGgtattgaaat131 8.94 3 NM_015180201109200946ttaaatgcagGAACTAGAACCTGCTTAAGGgtaagtcagc163 1.97 4 NM_015180198981198819tcatttgcagGTGGCCATACGTTACAGAAGgtaagggagg162 1.36 NM 197462197290cctttgccagGACTGCATGGTCGGATCAAGgtaagaaatg172 12.56 6 NM_015180184732184564atatgtgtagGGTGAAGAAGTGAGCAGCAGgtgggacaat168 5.79 7 NM_015180178777178584gtaatcacagGATCTACAGCGGCGCATGAAgtaagaacta193 0.48 8 NM 178101177949ctcccatcagAATCGAGGAGGAGGTTTGAGgtaaacacct152 0.36 9 NM_015180177591177405tgtgatgcagGCCTTTCAGCGAGACTCAGGgtgagctcct186 1.84 NM_015180175570175429acttttgcagCATTTCACCACCAACTGAATgtgagggctg141 0.71 11 NM_015180174718174522ctctcaacagGGCTTCCAACCTGCACTCCGgtacgggcac196 1.19 12 NM_015180173337173051tgtggtttagGGCTTGGAAGGCACTGTCAGgtaacagctg286 1.76 13 NM_015180171289171140ttcgtttcagGTAAATCCATACCACCCTATgtaagtctta149 2.00 14 NM 169139169013ctcattctagGGAAAGCTACCAGCAGTCAGgtactgcctg126 0.69 NM 168327168117ttaattccagGTGCCTTCGAGAGACTGCAGgtgagttaga_ 1.02 16 NM 167096166890tctctggtagGAGATACTGAGCAGTGCCAGgtacgctgac206 0.93 17 NM_015180165957165825gttttttaagGACTTCCACCGGAACTAATGgtaagtttcc132 1.48 18 NM_015180164342164149ctgttttcagCAACTGGAAAGGGAACCCAGgtgagtctac193 1.08 19 NM 163074162982tgaatttcagAACCCAGCCTCCGAGCAAAGgtaagaagcc92 0.45 NM_015180162537162482ctttacccagCAGTTCAGAGCAGAGAGCAGgtaacggggc55 0.27 21 NM 162214161092ctgttggcagGGTCCCCGGC- 1122-5 References 1. Ribeiro RC, Kushner PJ, Baxter JD, Tenbaum S, Baniahmad A. The nuclear hormone receptor gene superfamily. Annu Rev Med 1995;46:443-53.
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15 S. Schwabe JW. Transcriptional control: how nuclear receptors get turned on. Curr Biol. 1996 Apr 1;6(4):372-4.
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7. Green S, Walter P, Kumar V, Krust A, Bornert JM, Argos P, Chambon P. Human 20 oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.
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8. Menasce LP, White GR, Harrison CJ, Boyle JM. Localization of the estrogen receptor locus (ESR) to chromosome 6q25.1 by FISH and a simple post-FISH
banding technique. Genomics 1993 Ju1;17(1):263-5.
9. Mosselman S, Polman J, Dijkema R. ER beta: identification and characterization of a novel human estrogen receptor. FEBS Lett. 1996 Aug 19;392(1):49-53.
10. Enmark E, Pelto-Huikko M, Grandien K, Lagercrantz S, Lagercrantz J, Fried G, Nordenskjold M, Gustafsson JA. Human estrogen receptor beta-gene structure, chromosomal localization, and expression pattern. J Clin Endocrinol Metab. 1997 Dec;82(12):4258-65.
11. Murphy LC, Dotzlaw H, Leygue E, Douglas D, Coutts A, Watson PH. Estrogen receptor variants and mutations. J Steroid Biochem Mol Biol. 1997 Aug;62(5-6):363-72.
12. Moore JT, McKee DD, Slentz-Kesler K, Moore LB, Jones SA, Horne EL, Su JL, Kliewer SA, Lehmann JM, Willson TM. Cloning and characterization of human estrogen receptor beta isoforms. Biochem Biophys Res Commun. 1998 Jun 9;247(1):75-8.
13. Ogawa S, moue S, Watanabe T, Orimo A, Hosoi T, Ouchi Y, Muramatsu M.
Molecular cloning and characterization of human estrogen receptor betacx: a potential inhibitor of estrogen action in human. Nucleic Acids Res. 1998 Aug 1;26(15):3505-12.
14. Osborne CK, Yochmowitz MG, Knight WA 3d, McGuire WL. The value of estrogen and progesterone receptors in the treatment of breast cancer. Cancer 1980 Dec 15;46(12 Suppl):2884-8.
15. DeSombre ER, Carbone PP, Jensen EV, McGuire WL, Wells SA Jr, Wittliff JL, Lipsett M Special report. Steriod receptors in breast cancer. N Engl J Med 1979 Nov 1;301(18):1011-2.

16. Parl, Fritz F., Estrogens, Estrogen receptor, and Breast Cancer. IOS
Press, Amsterdam, Netherlands, 2000.

17. Birren B, Green ED, Klapholz S, Myers, R, Riethman H, Roskams J, (eds.) (1997), Genome Analysis: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

18. Ewing B, Hillier L, Wendl MC, Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 1998 Mar;B(3):175-85.

19. Gordon D, Abajian C, Green P. Conned: a graphical tool for sequence finishing.
Genome Res 1998 Mar;B(3):195-202.

20. Flouriot G, Griffin C, Kenealy M, Sonntag-Buck V, Gannon F. Differentially expressed messenger RNA isoforms of the human estrogen receptor-alpha gene are generated by alternative splicing and promoter usage. Mol Endocrinol 1998 Dec;l2(12):1939-54.
All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described modes for carrying out the invention which are obvious to those skilled in the field of molecular biology or related fields are intended to be within the scope of the following claims.

SEQUENCE LISTTNG
<110> Kalush, Francis et a1.
<120> Estrogen receptor alpha variants and methods of detection thereof <130> CL000258PCT2 <150> 09/692414 <151> 2000-10-20 <150> 09/768184 <151> 2001-01-24 <150> 09/804076 <151> 2001-03-13 <150> 09/826314 <151> 2001-04-5 <160> 2 <170> FastSEQ for Windows Version 4.0 <210> 1 <211> 465237 <212> DNA
<213> human <400> 1 ctgttgcttc acctcagaat cttgggagaa gcaggctcaa caatgaggga atcatccatg 60 acacccactg tcccaatgac catctttgcc tccacctgtt tgtgcccttt cacaatcatc 120 aagatattgt gatcttgatt tcgattcttt tggatacata tcctgaagtg ggaaggctgg 180 gttacatggt aataagagat atcagcactt ccatgtttat agcagcacta ttcacagtgg 240 ccaagatatg aaatacggta tatgcataca atggagtatt atgcagccat gaaaaagaag 300 gatattctgc ttcttctatc atatggcatg acattgatgg acctttagga cattatccta 360 aatgaaataa gccggtcaaa gaaagacaaa tactgcatga ttccacttat ataagatatc 420 tgaaatagtc acattcatag aatcaaagag agaaatggtg gttttcaggg gctggaggca 480 aagggaaaca ggggagttac taagaaatgg gcataatgtt ccagttaaac aagttgaata 540 agctcagaga tttgatatac aacattctac ctagaggtga caatgtacgg tgcacttaaa 600 aatttgttaa gggggtagat ctcatgttaa atgttcttac cacattaaga gaaaacaaac 660 aaacaaaaga tactgtagtg ttggagaatt ctcacaatgg ctgaactggt ctccactgag 720 cctggtggca aaagcagacg ggggaggctc ctctctcaga tcagttttgt agagttgagg 780 gaggggcacc ttgaatgaac ataatacttg gagagagtgt tgcagttaga ggacagaaaa 840 caaaagcaca tcatcaagag tgcctcctgc tatgtttatg cctattttac tgtccttttc 900 aaagaaaagc tttaagttta attgatcaac aatttttgaa tttttctttt agttgtgttt 960 tcaaatttat tgatttctgc atttctattt atttgttcct ttgttatgct tttttggggg 1020 gggaggctta ttttgttgtt ctttaatttc ttgagttgaa agtttatccc atttattttt 1080 agtctttttg gtttttaatg aatatattta agcctctgaa ttttttctct gaatactgct 1140 ttagtggtat tgcactagtt tttttttaac cccttgttct tggtttatta acagcctttt 1200 cagcataatt caatgcttat aacactgtct acatattgtt tttaaaatgg ttttgaagat 1260 tagttttttt aaaactaatt tctttctttt atttatttat ttttgagaca gggtcttgct 1320 gcattgccta ggctggagtc cagtggcgtg atcatggctc actgcagctt tgactccctg 1380 gcctcaggtg attctcccat ctcagcctcc agagtagctg ggactacaga aatgtgccac 1440 tacacctggc taatttttat atttttgtat tttttttgtt gttgttgttg tagagatgag 1500 gtttcaccat gttgcccagg ctgatctcga actcctgtgt ttaagccatc cacatacctt 1560 ggcctcccaa agtgctggta ttacaggtgt cagctaccat acctggctaa aaactaattt 1620 aaaacaacgt cattatggaa atacaattta cactgcttac agctcactaa ttaaaagtgt 1680 gcattttcat gttcttaaaa gtatattcaa aaagtttgtg accatcaaca caatttcatt 1740 ttagaatgct tttgtctttt ctaaaagaaa cccatccttt tcctcccaca actttcccta 1800 cctttaagcc accagtaatc tactttttgt ctttatgtat ttgcctattc tagaaatttc 1860 atataaatgg aatcatataa tatgtcgcct tttgtgactg gctgctttca cttagcataa 1920 cttttttgaa gttcatccat attgtagtgc ttcagtcctt gttatggggg aataatattc 1980 cattgtatgg atatactaca tttaaaaaat ctatttgtta ttggtaaaca ttagagctgt 2040 ttctactttt tggttattat gagtaatgct gctaaaaaca ttgtatacaa gttcttgtgt 2100 gaatgtgtgc ttttatttgt cttgttgata tacctaggag tggaattgtt aggtcacata 2160 gtaatgctat gtttaccttt tgagacattg caaaactttt ttccaaagca gctgcaccat 2220 tataaattcc caccagcaat gcatgaggat tcaatttctc cacattgttg tcaacacttg 2280 ttactgtctg gagccagcct agtgggtgtg aagtggtatt tcattgtggt tctgatttgc 2340 atgtctctaa taactaataa tgttgagcgt attttcatgt gctcactggc catttgtaca 2400 tcttctttgg aaatatatct attcacccct tttgcccatt ttaaatgttg tattattttt 2460 ctttttatta ttgcattgta agagctcttt agatcttcta gatgcaaatc ttttatcaga 2520 tatatgattt gcaaatattt tctactgttc tgtgtgttgt ctttcatttt taaataatat 2580 tctgtgaagc caaaagtttt aattttgatg aagtctaaat tatcattatt ctttgttttg 2640 ttgtttgtac ttttggtgtc atagctaaga'aataattgcc taatccaatg tcatgaagat 2700 ttatgtcttt ctttcttttt ctttccttct ttctcttttt ctttctttct ctttctttct 2760 ttCtttCtCt ttCCCCttCC ttCCttCCtt CCttCCttCC ttCCttCCtt CCttCCttCC 2820 ttccttcctt cctatctcgc gctctctttc tttctctctc tgtctctctc ttttttttag 2880 gcaaggtctt attatgttgc ccaggtgggt cataaactcc tgggttcaaa agtgatactt 2940 tcatctcagc ctcctgagta gctgggacta caggcatatg acactgtgcc tggattaaga 3000 gttttataat tttagctttt gcccttaagt ctttgatctg ttttgagtta aaatttgtat 3060 agcgtgaggt aggtgtccaa cttctttctt ttgcatgtgg ctatctagtt gctccagcac 3120 catttgttga aaaggctctc tttctccctt gaattttctt gttgcccttg tcgatgtcag 3180 ttgactataa attcctgagt ttatttctga attctcaatt ccactccact gatctaagtt 3240 tattcttatt ctagtagtca ccattatttt gatcttcttt ctgtgtctca gattttttcc 3300 ccctgtagaa catctccagt aatggtttat ataagtttgt gattagtgat gttatagtgt 3360 gataatgtgt ttatttgctc ttacactttg gctgagctta gaaatatttg tattttgacc 3420 tttgatacgg tttggatatt tgtctcctcc aaatctcatg ttgaaatgta atcactaatg 3480 ttggaggttg ggcctaatgc aaggtatttg ggtcatgggg gtggatccct catggcttgg 3540 tgctgtcctt gggatagtga atgagttctc atgagatctg gttgtttaaa agtgagcggt 3600 acctccttcc tctctctctt gctcctgctc tgccaagtga tgtgcctgct tccgctttac 3660 cttccaccat gagtaaaagc ttcctgaggc ctcaccagaa gcttagcaga tgcaggtgcc 3720 atgattcctg tatagcctgc agaactgtaa gccaattaaa cctctcttct tgataaatta 3780 cccagcctca tatatgtttt atagcaatgg aagaatggcc gaatacaacc tttgagatgt 3840 ctgtttcact gtcatctttg cttcagagtt actgaagaaa agtctgatga tctcattctg 3900 ttttcttagt tgataattgg tttttatttt atctctttct ctgcaagact ttcagatttt 3960 ctctttatat ttagcgttct gaaattttta cagtactgtt ttgcgtgatt cattttttat 4020 tcatcttttg tcttttcaat ctgaggattt ttgtttgttc tggagtattt ctttattatt 4080 gctttgagta ctgcctttct tccattctat tctttccttc aggcactcat attttcaaat 4140 attagaattt ttggatctgg tctaaaaagg aaataaaaaa atctataatc agactagaat 4200 agaaggggta cccttgacag tccaagaaat aagagaaagc cctgacatgc caaacagatg 4260 ttacgagata aacttgtaag acagaaatac tgcagctggg gcgcagtggc tcatgcctgt 4320 aatcctagca ctttgggagg ccaaggtggg tggatcactt gaggtcaggt gttcaagacc 4380 agcctggcca acatgatgaa accctgtctc tactaaaaat acaaaaattt gcctggcctg 4440 gtggcgggcg cctgtaatcc cagctactcg ggaggctgag gcaggagaat tgcttgaacc 4500 tgggaggcag aggttgccat gagctgagat agtgccactg cactccagcc tgggtgacag 4560 tgagagactc catttcaaaa agataaaaaa tgacacaaga aagatgatag aaatgacagg 4620 atgtggagac acaactcaaa ttctaggttc cagatcttga catcttccca ctgtcttact 4680 gctctgcatc atgttagctt agaaagttac tcaacctctt tgatctttgg attcatgatc 4740 taaaaatgga gtgttggggg gcgtggttaa agtgataatt ttgaataagt cttcaaaccc 4800 aacatgtcat gagccttcct tgaattgcat cttcatttat agattaaacg ttttgataaa 4860 ttcactatgg gctcttaact gtattattaa aattgtggtg acaaccagct gttattttat 4920 ggtagaacat aagtatctct tttcattgcc attcttcact aaaaggatac tttgtccccc 4980 tttttctttt tccataggtt attggggtac aggtggtgtt tggttatatg agtaagttct 5040 ttagtggtga tttgtgagat tttggtccac ccatcacctg agcagtatac actgcaccct 5100 atttgcagta ttttattcct tgccccctcc aacccttccc tccaaatccc caaactcctt 5160 tgtatcattt tatgcctttg catcctcata gcttagctcc catataacag taggaacata 5220 tgatgtttgt ttttccattc ctgagttact tcacttagaa taatagtctc caatctcatc 5280 caggtcactg cgaatgccgt taattctctt ctttttatag ctgagtagta atccctagta 5340 tatatatact acagaatctt tatccactca ttgattgatg ggcatttggg ttggttccac 5400 gattttgcaa ttgtgaattg tgccactata aacatgcgtg tgcaagtatc ttttttgtat 5460 aatgacttat tttcctctga gtagataccc agtagtggga ttgctggatc aaatggtagt 5520 tctacttttg gttctttaag gaatctccac gctcttttcc atagcggttg tactagttta 5580 cattcccatc agcaatgtag aagtgttccc tgatcaccac atccacgtca acatctactg 5640 ttttttgatt ttttgattat ggccgttttt acaggagtaa ggtggtatgg cattgcggtt 5700 ttgatttgca ttgccctgat cattagtgat gttgagcatt tttttttgaa tgtttcttgg 5760 ccatttgtat atcttctttt gagaattgtc tattcatgtc cttagcccac tttctgaagg 5820 gattttttaa aattgctgat ttgtttgagt taattgtaga ttttggatat tagtcttttg 5880 tcagatgtat agattgtgag gattttctcc cattctgtgg gttgtctgtt tactctgctg 5940 actgttcttt ttgcggtgca aaagctcttt agtttaatta ggtcccggct atttatcttt 6000 gtttttattg aatttgcttt tgggttcttg gtcatgaaat ccttgcctaa gccaatgtct 6060 agaagggttt ttccagtgtt atcctctaga atttttagag tttcaggtct gaaagtcctt 6120 aatccatcta gagttgattt ttgtataagg tgagaaatga gaatccagtt tcattctcct 6180 acattcggct agccaattat cccagcacca tttgttcaaa agggtgttct ttccccactt 6240 tatgttttca tttgctttgt tgaagatcag ttgactgtaa gtatttgggt ttatttctgg 6300 gttatctatt ctgttccatt ggtctatgtg cctattttta taccagtacc atgctgtttt 6360 ggtgactgtg gccttataat acagtttgaa atctggtagt gtgatgcctc cagatttgtt 6420 ctttttgctt agtcttgctt tggctatgca ggcttttttt gggttccgta tgagttttag 6480 aattgtgtct tcaagttctg tgaagaatgg tggtatttta atggggattg cattgaattt 6540 gtagattgct tttggcagtc attttcacaa tattgaatct acccatgcat gagcatggga 6600 tgtgtttcca tttgtgtcgt ctatgatttc tttcagcagt gttttgtagt tttccttgta 6660 cagattcttt cacctccttg gttaggtata ttcctaagca ttttatttca tcttttgcag 6720 ctatcataaa agaggttgag ttcttgattt gattctcagc ttggtca,ctg ttggtgtata 6780 gaagagctac tgatttgtgt acattaatct tgtatccgaa actttgctga attcttttat 6840 cagttctagg agctttctgg aggagtcttt agggtttttg aggtaaacaa tcatattgtc 6900 agcaaacagc gacaatttca cttcctcttc gtcaatttgg atgcccttta tttctttctc 6960 ttgtctgttt gctctagcta ggatttccac tactatgttg aagaggagtg gtgagaatgg 7020 ccatccttgt cttgtttcag ttctcagagg gaatcctttc aacttttccc cattcagtat 7080 tatgttgcct gtgggtttgt catatatggc ttttattata ttgaggtatg tcccttgaat 7140 gatgattttg ctgagagttt ttatcataaa gggatactgg attttgtcaa atgctttttc 7200 tgcatctgtt gagatgatca tatgattttt gtttttaatt ctgtttatgt ggtgtatcac 7260 atttattgac tcgtgtatgt taaaccatcc ctgcatccct ggtatgaaac ccacttgatc 7320 atggtgaatt atctttttga tatgttgttg gattcagtta gctagtattt tgttaaggat 7380 tttagcatct atgttcgtca gggatattgg tctgtagttt tctggttatg tcctttcctg 7440 gttttggtat taaagtaatg ctgacttcat agaatgaatt agggatggct ctttctttct 7500 ctattttgtg gaatagtgtc aataggattg ctaccaattc ttctttgaat gtctggtaga 7560 attctgctgt gaatctgtct ggtcctggac ttgtttctgt tggtaatttt taaattacca 7620 tttcaatctc actgcttgtt attggtctgt tcggggtatc taattcttct tgatttaagc 7680 taggaggttt gtatctttcc aggaatttat ccatctcttc taagttttct actttatgtg 7740 cataaaggtg ttcatagtag gcttgaatga tcttttgtat ttctgcagtg tcagttgtaa 7800 tatctcccat ttcatttctt attgaggtta tttggatttt ttctcttctt ttgttggtta 7860 atcttgctaa tcgtctatca gctttattta tcttttcaag gagccagctt tttgtttcac 7920 ttatcttttg tatttttttg ttgttgtttc aatttcattt agttctgctc tgatcttggt 7980 tatttccttt cttctgctgg ggttgggttg ggtttgttct tgtttctcta gttccttgag 8040 atgtgacctt agaatgtcag tttgtgttct ttcagtcttt ttgatgtgga tgtttaggga 8100 tatgaacttt cctcttagca ccgcctttgc tgtatcccag aggttttgat aggttgtgtc 8160 actattgtcg ttccgttcaa ataatttttt gatttccatc cgatttcgtt tttgacccaa 8220 tgatcattca ggagtaggtt atttaatatc catgtctttg catggtttca aatgttcctt 8280 ttggagtcta tttccagttt tactccactc tggtctgaca gagtgcttga tataatttca 8340 gttttcttat atttatggag gcttgtttat gacatatcat atggtctatc ttggagaaag 8400 ttccatgcac tgttgaatag aatatgtatt ttgtggttgt tggatggaat gttctgtgta 8460 tatctgttaa gttcatttgt tccaaggtat agtttaaatc cattgcttct ttgttgactt 8520 tccatcttga tgatctgtct agtgctctca gtggagtatc gaagtccccc agtattattg 8580 tgttgctatc tatctcattt cttaggtcta ttagtagttg ttttataaat ttgggagctc 8640 cagtgttaga tgcatatata ttttcctgat ggacaaggtc ttttaccatt atatactatc 8700 cctctttgtc ttttttaact gctgttgctt taaagtttgt tttgtctgat ataaaaatag 8760 ctacccctac tcacttttgg tgtccatttg catgaaatgt ctttttccac cccttacttt 8820 aagtttatgt gagtccttat gtgttaggtg agtctcttga aggcaacaga tagttggttg 8880 gtgaattctt atccattctg cagttctgta tcttttaagt ggagcattga ggccatttac 8940 attcaatgtt agtattgaaa tgtgaggtac cattgcattc atcatgctct ttgttgactg 9000 tgtaccttgg ttttttgttt ttaaattgca tttttatttt ataggtcctg tgttatgctt 9060 taaagaggtt tctgttttga tgtgtttcca ggaattgttt caagatttag ggctcctttt 9120 agcagttctt gtagtggtgg cttggtagtg gcaaattctc tcatcatttg tttgtctgag 9180 aaagactgta tctttccttc atatgtgatg cttagtttca ctggatacaa aattcttggc 9240 tgataattgt tttgtttgag gaagctaaag atagggcccc aatcccttct agtttgtagg 9300 gtttctgctg agagatatat ctgctgttaa tctgataggt tttcctgtat agattacctg 9360 gtgcttttgt ctcacagctc.ttaagattat ttcctttgtc ttaactttag ataacctgat 9420 gacaatgtgc ctaggtgatg gtctttttgc aatgaatttc ccagttgttc tttgtgcttc 9480 ttgtatttgc atgtctaggt ctgtagcaag gctggggaag ttttcctcta ttattccccc 9540 aaatatgttt tccaaacttt tagaattctc ttcttcctca ggaacatcaa ttattcttag 9600 gtttggtcat tcaacataat ctcagacttc ttggaggctt tgttaatatt ttcttattct 9660 gtttcctttg tctttgttgg attgggttaa tttgaagacc ttgtctttga actctgaatt 9720 tctttcttct actgttcaat tctattgctg agactttcca gagcatttta catttctata 9780 agtgtgtcca gtgtttcctg cagttttgat tgttttttct ttatgctatc tatttccttg 9840 attatttctc ccttcgcttc ttctattgtt ttttggattt ccttgcactg gggtttggct 9900 ttctctggtg cctctgatta gctgaataac tagccttctg aactcttttt tcaggtaaat 9960 cagggatttc ttctcagttt agatccattg ctggtgagct agtatgattt ttttgggggt 10020 gttaaagagc cttgttttgt catattatca gatttggttt tctggttcct tctcatttgg 10080 gtagcctctg tcagagggaa gttctatggc tgaaggctgt tgttcgtatt cttttatccc 10140 acgaggtgtt cccttgatgt agtactctct cccttttcct atggatgtgg cttcttgaga 10200 gccgaactgt agtaatggtt atctctcttc caggtctagc cactcagcaa gtctaccagg 10260 ctgtgagctg gtattgcggg ttgttggcac tgagttctgt ggtgtgaact gtctgtgtgt 10320 ctctccgcca tggacacctg cacctgttat gggggaggtg gtagaggggt gaaacggatc 10380 cctggttctt agctttggtg gtttaatgct ctatttttgt gctggttggc cttctgccgg 10440 gaggtggcgc tttgcagaca gcatcagcta tggtagtgtg gagagggact ggcagtgggc 10500 ggggtcctag agctcccaag aatatatgcc ctttgtcttc agctactagg gagggtaggg 10560 aaggaccatc aggtgggggc agggctaggc ctgatctgag ctcagaccct ccttggatag 10620 gtcttgctgt ggctgctgtg aggggatggg agtgaggttc ccaggtcaat ggaattgtgt 10680 acctaggagg attacggctc cctctgcgga gtcatgcagg ttgtcaggga agtgggagaa 10740 agccaacagt caccagcctc acccagctcc tgcgcaatcc aaaggggtgg tctcacgccc 10800 actgtgtccc cgctaacaac tctgagtctg tttccaggca gggggtaagc agggctgaga 10860 acttgccgca gtctgcccac ctcccagtgg caaaagaaaa gggctttagt tcttccccag 10920 cctgtggagt ctgcacgcag gattcgcacc ctcccccgtg ttatggccag gaggcttctt 10980 gaccggttca aattgttaca aagttcagct ggagacttcc ttcttcctgt ggtgtttccc 11040 ccgtgcctct ggccaccctc ctgaaggatc cttgtgatgc caggcaggag tggcctgctt 11100 ggggacccag cgagctcccc aggcctttcc agctgcttcc tctatccctg tgttttgctt 11160 gtctatctaa attgactcag ctccagataa ggtcagaatc ttctcctgca aactagaact 11220 tcagtttccc cataggaggt gtgcgttcca gggcggagga tctccctttc ccacttccgc 11280 agttggggca ctcacataat ttggggtgtc tcctgggtcc agcaggagca gtccgcttcc 11340 ttcagagggt ctgtgggtcc tcctgggatt cctggtttat tcctgcagtt gttctggagc 11400 tgaaattcac aatgtgagcc tctgcacgct gttctgtgcg tccgagtcga agctgccatc 11460 tagttttgcc tcccatccgt ggtgatgatc tatgatctct acaaggatac atttgctaga 11520 aatatttgtt ctctctggcc gggcgcggtg gctcacgctt gtaatcccag cattttggga 11580 ggccgaggca ggtggatcac gaggtcagga gatcgagacc atcttggcta acacggtgaa 11640 accccgtctc tactaaaaaa tataaaaaat tagctgggcg tggtggcggg cgcctgtagt 11700 cccagctact caggaggctg aggcaggaga atggcgtgaa cccgggaggc ggagtttgca 11760 gtgagccgag atcgcgccac tgcactccag cctgcgcaac agagcgagac tctgtctcaa 11820 aaaaaaaaaa aaaaaaaaaa agaaatattt gttctcccta ccctaaaagg gatataattg 11880 aaatacaaaa aaacaatatg gagaggtaat ttctttcttt ctttttcttt ttcttttttt 11940 tttctttttg gagacagggt cttgttctgt tgcccaggct gaatgcagtg ggacaatcac 12000 agctcactgc agccttgacc tcctgggctc aaatgatcct cccaccttag cttcctgagt 12060 agctgggact acagatacgc accaccacac cagctaaggt ttaaaattgt ttttaatagt 12120 gagagaattt cacaattttg ccaggctggt ctcgaactcc tgggctcaag caatctgccc 12180 acctcggcct cccaaagtgc tgggattata ggcatgaacc actgcaccgg aaccttggag 12240 aggtaatttt taacaaggcc atcttttctc taacagagga gcactaagaa gtcagatttc 12300 atcctggcat gtgactgtca cacaagcaaa tgactgtgct gattagaaag cttgtgtagc 12360 tcagaaatat cacataatat gcagtctccg ttgagcaaca ttgcagctta tgttcattag 12420 gtaaaaccat agtaatctga aaatatttta ggtatatctg ccattatttt atgactaaac 12480 aaggagatga ttttagtatt gattactttc atgcacatat tttaaaaact cctgattttt 12540 tatgttggaa acattccagt tctttttttt tttttttttt tttttttgag accggagtct 12600 cgctctgtcg cccaggctgg agtgcagtgg cgggatctcg gctcactgca agctccgcct 12660 cccgggttca cgccattctc ctgcctcagc ctccccagtt cttttcttat ctagctattt 12720 tgaactatac atttgattgt attccaaaaa tacgtacaac tattatgtat caataaaatg 12780 tcctaacttt tttgcatagt gaaaggtttg ttcaggttat agtatgagtt gcagtcatcc 12840 tgtttttggc atgggaggga gatgtttcta aactagccag ttataggtgt cacctaccaa 12900 tgttctacaa aatgtggacc aggcagagag aattacagat gctgggtgga aactttggtt 12960 ctgggcctga ttctgcccct aacttggcgt gaagcctcag tttcttctta ctacagcagg 13020 aaaaataata cttgctttat ctgtttcatg tgtctgctat gatgataaca aatataatgt 13080 atatgagaca atttaaaaac ttaaagcatt ttaagttcct tggtctttat gggagaggga 13140 ggaccatcat ttctttctct tttttctttt tcttcttctc ctcccctttc ctcctctttt 13200 tttcattcct tctttttctc cttatctttc tcctcctccc tctcctcctt cttcttcctc 13260 CtCCaaCCtC C'tCCttCtCC tCgCttCttC CCttCtaCtt ttaCttCCtt CttttCtcct 13320 tCt CCtCCat tCtCtttCtC C'tCCt CCttC atCCaCtCCt tCttCttCtt ccttttgaga 13380 tggaatcttg ctctgtcacc caggctggag tgcagtggca tgatcttggc tcactgcagc 13440 ctccacctcc cagattcaag tgattctcgt gcctcagcct ccctagtagc tgggattaca 13500 ggtgtatgcc accacaccca gctaattttt tttttttttt tttttttttt gagacactat 13560 ttcactcttg tcacccagac tggagtgcag tggtgtgatc ttggctcact gcaaactcca 13620 cctcccagat tcaagcaatt cccctgcctc agcctcctga gtagctggga ttacaggtgt 13680 gcaccaccat gtctggctaa ttttgtattt ttagtagaga tggggtttcc ccatgttggc 13740 caggctggtc ttgaactcct gacctcaagt gatccacctg cctcggcctc ccaaagtact 13800 gggattacag gtgtgagtca ccgcacccgg tcctttcctt cttcttcctc ttcttcttct 13860 tcttccttct tttctctgtc tccttctcct ttttttcctc cttgtccttc ttctgtctga 13920 tgcatagtca agggtttgat aagtgtctgc tctgtgctga cagtttcagg agcagtacag 13980 aaactcctat tttgtactcc aaataatcca caagaattaa ttggatcacc tcgaaggagg 14040 aaagagaagc tcaaagagat caggtaagtt gcacaagctc tgaatcccag cacaccgtga 14100 aaacgggctt caaatctggt ctttctgatt ccaaagtcta tagtcttacc tgtgattcac 14160 tttcctgatt tgttaaaaaa aaaaaaaaaa aaaaaaggat gcttcatttg cttaagtaaa 14220 attccaggga attttcagca gaagttttaa atgttggtag agaattttct tgaggcaaaa 14280 ctgataatca taaagcttgt ctggctacca aaattgatct ggtagtcaaa acttcttggt 14340 aaagttatca gtatagagac ccaagagaaa caaataagaa gaatctgcta ctaaattttg 14400 gcggagttga gaaatttttt aatttgagct ttcactctaa gagataagag tctgtgacca 14460 attgtattgt gtgttttgca ttgctatgaa ggaatgcctg aggctgggta gtttataaag 14520 aaaagaggtg tatttggctc agagttctgc agcctataca agcagggcag cagcttctgc 14580 ttggcttctg gggaggcttc aggaagcttt tgctcatggc agaaggcaaa gagggagcag 14640 gcatgtcaca tgagagggag caagagtggg ggaggaggtg ccaggctcct ttaagcaacc 14700 agctcttgca tgaactaata gagtgagaac tgaatcatta ccatgaggag gacaccaact 14760 cattcatgag agatccgccc ccatgaccca aacacctccc accaggcccc acctccaaca 14820 ctggggatga catgtcaaca caagatttgg acgggacaaa tattccaact attcacccat 14880 gttccctaac ttgaagggag ttcacattag gacaagttca ctgaaatctt tgttttggaa 14940 tggggatgga taagacatca tttattaagt tatggttttt agtttctggc tttggctaac 15000 tcaaacaaaa agggaatatt tcagactggt gcttggtaaa tcatagaatg acaaacaatt 15060 ggaagaccag gttaaaatga acttggagga cattaagtga attaagccag gcacagaaag 15120 acaaatactg caaattctca cttatatgtg gaatctaaaa aaagttgagc ttatacaaac 15180 agagagtaga atggtggtca ccagaggctg gggaaggagg agttgaggag atatcggtca 15240 aagcatacaa aatttcagtt aggcaggagg aataaattca agagatctat tgtacaacat 15300 ggtgactgta gttaataaca ttgtgactgt attgaattct tgaaagttgc taagagagta 15360 gattttaagt gttctcactt aaaaaaataa gtatgtgaga taatgcgtat gttagttagc 15420 tctatttagc catttcacaa tgtgtatgta gtccaaaatc atgttgtaca tgattcataa 15480 atacattttt atttgtcaat taaaaatacc ctggaatttg ggcaacaaaa gctatctgcc 15540 caggtgggaa caaatcgcct ctgagcatct ttccttcatt gcatctctaa tcaagataga 15600 aagtccctgg agagagggtc tgattggaca agcttgagtc cgatccctat actttggcta 15660 tgagaggaat attcttgctg aagtcgtctg tgatagaccc tgtggatgca tttggcttgg 15720 aaatacgaaa gcgccttgat ttagaatccc ctcaagtctg tctgttcaca gtgggataat 15780 ttttattctc ttcctctcca gaaggaatca aggggtgtca taaaggaggt atgtctgcta 15840 agcagaacta cctcccctcc ccaaagaaag tctgttacca tgtatacttt taaacatttt 15900 attttaaaat aaaattagac tcacaagaag ttacaaaaac agtacagaat tgccatgtaa 15960 cttttaccca gctccccaca atgataccat cttatataac tctagtacat tattaaaata 16020 tggacattga caccggtaaa atactattaa cagaaccaca tactttattc tgcttttgct 16080 ttttatttta ttttattatt ttattatttt ttgagacaga atctcgctct gtcacccaag 16140 ctggagtgca gtggtgccat tgcaacctct acctcctggg ttcaagcaat tctcctatct 16200 cagcctccca tgtagctggg attataggtg cccaccacca tgcctggctg atttctgtat 16260 ttttagtaga gatggggtgt caccatgttg gccaggctgg tctcgaacgc ctgacctcaa 16320 gtgatctgcc cgcctctgcc tcccaaagtg ctaggattac aggtgtgaga caccacgccc 16380 agcctgtttg gcattttttt aatacgtgct ctataatatt gatttttgtg taaacatgtg 16440 tttgtatata tctataacga gaactcaagt catactgtaa tcctattttg taaactgact 16500 tttttccttt atcagtatat caagattatt ttcccacatc atttgacatt ttttctacag 16560 tgtaatttaa tggctacatt gttttctatc ctatgaatat atcaaaccta tttcttaaaa 16620 accctactca gggattttaa aaaataaaaa cgatgtttta atattataaa gattcagtga 16680 ggtatattct tatacgtaca catttctaag gtttgagttc ttacaagatg ctgaactagc 16740 taagactact ggttctcatc tgtcacatag ggaaaaatta tagaaggaaa acatcaagat 16800 ttggaaaaat ctgtgagaat tgttttgcat tagtgtgtag gtgtgtgtgt tggggtggtg 16860 gctgcagctt ggggcagagg cctcaggtgt ggctgtggag tgatcagata gagtttttgg 16920 agttcggctt ttgccccagg acacttggtg cctgccccca gagctgcagc ccagaaggcc 16980 gttctcagag gtgaagtcca ggcagtgagg agctgtctgc cagtaggcag ttgaagaaaa 17040 aaaatgagct agaggaaaaa aacaaaaaaa caaaatctcc ttctaatgct gccaggctgc 17100 cgggagctgg aaatgaagca ctgacaggag tgggtatttc atggtgaagg gaataatcaa 17160 ctggtttttt tggtacccaa gactttccac cttcacacac acacatgaga tgctttgaaa 17220 taaagatagt cacttgactt agtaaagttt gttgacataa aaatatgaga aataccaaag 17280 aatacaaaaa ggaaaacttc gttaatatta ttcagactta aaattccaga ttgtatcaac 17340 attaaggggg ttgatgaaaa catgggagaa agccaaggga cgtgagatcg ggctcaattc 17400 ttgacttgct gggggaaggt atcaacacag aacttttaag aattagaagg cattaaaaag 17460 aaatagaaat cctgaatcaa attgaaacag taaaataaaa tagtccaaag atgtgtaaat 17520 atatcactat cacaataact ataaataggt taaatttgcc agttgaaaga aaggaactat 17580 taaatgagat tttaaaaatt tggatatatg cttttcatgt gaacatacct aaagcatata 17640 cacaaaacca gaaaataaaa agatggaaat atccacagag tgacaaagga aagctggtgc 17700 atttgtatta gtatcagata aagctcacat ttagataaaa aacattatag taatagagaa 17760 agtcacaagg ttaaagtttc aattcaccaa gaaatatcca ttctaaacat gtatacatac 17820 ccaattaagc tgcctcaaaa tatatatgac aaaaattggg agaactataa agtcagatat 17880 aaggattgaa aagaaagaaa tgaaactaat tattttcaga tgatatacac gtttacatga 17940 aaaaactcca aagaatcttc aggcaaatta ttagaaattg taggagagct ccgcaagagg 18000 gctggatata aaattaatat acaaaagtca atgtcatttc actataccag aaaaaagaca 18060 ataacattag aaaataaatc taacataaga tgtagaaaaa cttcttagag gaaaaatttt 18120 cttgggaaat attaaagaag atctaaataa gtagaattac ataatattca tgcataagaa 18180 ggcaacattt tgaagacgtc ttgttataca taggcctagg aataatatag ttttaagcta 18240 tattattttg aactgtagat gaagaaaagc tctttttttc cttgagtatt ttaagatttt 18300 gaataaagtc atattgctct aggaatattt tatacttctg ccagcagtgt ttgagaatgc 18360 ctacttccta cattatcacc accataaatg tttaaaacct gatagcacac agtttaaaaa 18420 atttgtaatt ctccaattaa cagtgacctt taaaaattat gtttactgat ctctattatt 18480 ttgtcctttg taaatctttt attagttttt atgttcacat gttccgtttt aaaatagact 18540 tttaagagct ctttgtatta agaatattaa ggctttgtcc atataattag tatttttcag 18600 aatacccttt ctaggtacag tttttcagtt ccagtgcaca gaatggaaac ttgctacctg 18660 ct acaccaca ttttcactag tatgtgatgt attcatcaca aacaacagta ttggccaaca 18720 ttttccctca ctgtgaagtg cacatttgac atcctttaga aaaattactg acggttttga 18780 gacgattgtt ctgtgctttc tttcagtcag cataattttc ccgaaagcag agatgactct 18840 tccagacttg ctaccaatgc ttgaacaaac tgtgtaagct tagtccaaaa aaaatatttg 18900 attaatagat tttattttgg tagattctaa ggttccaagc agtcagagaa ataatcgcag 18960 agcctcaaat atctccaaaa tctgatacca atccttttga ttgtgaatta tattctgtag 19020 ctaccaaaga aggtaagttt ttattttttc tactctatta actttccctt ggacaactga 19080 atattaagat gactatgtaa ggaggttatc agaccaaggc ctcacacatc aggataaaag 19140 cacatgccat agaaagaaca tttgtgtctc aaaaggtgat accaagacaa ggctgtggga 19200 tatatatggg cacaatggtt gataccttca aagacttcat acatggtgtg gaggtttttg 19260 gagattttaa tttataatga caatctttcc agttaggaga atttttggac tgtaaagtta 19320 gccaaacaac ttttcaatga taataaatgt ctatatctta,cacaggggga atacagtttt 19380 tgtttgtttt agttttttct ctgtctattc atataggggc atcatatgtc tttatcatta 19440 aaaactgatt tagataggca gttgtagaga aatttaatgt gtgggaattt aaagttttaa 19500 aaagatgtag caaaatatag atacatttaa gacccactgc accaatgaaa aagggaaatt 19560 aattaaacta gacaactttt atgggtacta accattaaaa ttaaaatttt agtcattgta 19620 aattacatta cattataata gttttcaatc tattgttaag ttaaatattt tgggtaacaa 19680 ttgtatttca cagttgttca cactttgcat aattttctaa ggaaaatgag tcccttaggg 19740 agaaaattgt tcttaaattt tattgttgga taatgttgct gtactaacat ggtttactga 19800 agtggatcta ccatgggtgt ttaggttttt gttctttaca aaatgtattg ggtgaaattt 19860 tccatatcac tggtgaactt ttcatgctaa ttttccagtc ccttagttta aatattatgc 19920 attttttcct gcaaatctat cttgaatctg aaaaggcccc agaaggaatt agcgtgagtc 19980 cagagtagat ggactgcaaa tgttacacat atttcaatat gtatccagct ttcaaaaata 20040 gcttattgaa atcaaggttg aaccacatga aattttgggt ttttttggat gtaaaaaatg 20100 gttatatatt ggcattttca tatcatccaa tctaatatct tcctctagtg aggttctggc 20160 aaaaaaaaag tatgatgacc agagttgttc actcttttaa gctttatgtt tcaaaatatt 20220 tttaaaattt aaaaccatct aagtgcatcc caaaaaacat tggaaaattt tgtaggctag 20280 ttttgtttaa tgatttttct tctatagatt tatttttatg ctatgcctta ctctccaatt 20340 agattttagc catccaagaa aggtaccttg tacttctaag cgattctccc atcacctgga 20400 cgttaactgc agcccacact gcagtgtctt gactgcagta ggcactcagt aaatatttat 20460 tgatggtcct gattatggga tgatgaaata tgctaaaggt aacttaaact tttgtcaata 20520 aaaccgagtt tcaggttagt ttgacctttt ccgtatctct aaatattttc tttcatgtca 20580 tttttgtgaa atagctcacg aaagtactta ttgattctga aatccttttc ccctctggta 20640 ttcctgagct atcctcactc acacacagca caccagtgct attgtttgct gttttgttaa 20700 aatcattctc attaatattc ctcattcaat aaaaatagtt gattcccact tatgcaccaa 20760 actctattct aaagactgct gtttatttta tggagtcaat atcatcattt tgttttccat 20820 tccacataat agttggatgc caatatgaag tgtatattta taaataatat gtgctttttt 20880 tatttgttta tttttatttt actttaagtt ctgggattaa tatgtgcttt taacattatt 20940 gtttaaaaat aacaggagat atcatatctt aagcaccttc tatattctgg gcactgttct 21000 aaatgtatca aagttatcat ctcatttaat ccctccacac acctgaggga agagagtatt 21060 attccttatt tacagataag gaagctgagg cttggaagat tcatatatct aagatcacaa 21120 agctagtaaa gcagccaagt ttgagtctaa atccaggttt taaatatcat attgcatgtg 21180 ggcatagatg agcaagaaca ggttttcctg aagatggcca gtctaaggct tagaagaagg 22240 aagctcgggg agcctaaacc aagatatgct aaggcaagtt tttttgctcg tttgtttttg 21300 agatggagtt tcgatcttgt tgcccaggct ggagtgcagt agtgtgatct cggctcaccg 21360 caacctccgc ctcctgggtt caagtgattc tcctgcctca gcctcctgag tagctgggat 21420 tacagtcatg tgccaccatg cccagctaat tttgtatttt tagtagagat ggtgtttcac 21480 tatgttagCC aggCtggtgt CaaaCtCCtg aCCtCaggtg atCCgCCCr'3C CtCagCCtCC 21540 cagagtgctg ggattacagg cgtgagccac tgtgcccggc ctgctaaggc aagtattgac 21600 cattagagga gatgactagt cccagtggca ggcctttcct actgggagtc agccaagagc 21660 tCCCCdCtCt tCtggttCCa ttgttcctcc tgcaaagttc cattcataaa gtggtttccc 21720 atttgattgt ccaatgatgc aaatctccag tcctttttag cactgagttt ctcatagcct 21780 tttaccattg agtttctcat ggctaatgaa tgctaatgaa ttggcattcc gatttatcta 21840 taggattcca tgagtcagcc tcatgaaaga aggttccact ccctcactgt gggtaccgtc 21900 tgggaattag acccttaata ggagtaattc agcaggcaat gtcttctcta ataacactcc 21960 agtctcactg agagcttcta ttcaaaacaa caatctaaag gtctacagtg tgactttcaa 22020 ctaaactgaa aaaagaatag aaaatgaatg tatgtgatgt acacattcat tcaaatgttt 22080 atccctaaaa ttcatagcag attatgactg atcattaaag ccaaattaag ggggcaaaat 22140 gtataaaaac aaaaggaact cttaggcata atagtcttta aagtaaaaca ttactactga 22200 cagttgcttg tgtgaaagat atctttattt aatatggtaa aaagaacaca ggaattgcta 22260 gcacttaccg tatgtccatt ttatgtccac cttaaaatag atatgtctgt atagtcatca 22320 gattagcact ttagaattca ataatttcaa agaaatcaga atggacattc attacatgtg 22380 cttatctggc aggagaattg aacaaatcct ttctatagga aaatgtaaaa ttcctaataa 22440 ctccacagga ttgtaagact tgggctggta acttcagtga atacagcaga gtttgggcga 22500 tgggaaaagg tacagttgtg ttcctaaact cttaactttg tgaggcagtg actttatttc 22560 ttcttcatga ttttgcagta agttcagtac agtggttagt ttcagtatag ttcagtggct 22620 aagcttttga gagggacagt tctatgttct aactcaactt ctgccacttg ttagaaagtg 22680 actcttgtca aactattggc cctttcaaac ctcagtttct tcatctgcaa aactttattc 22740 atggggttgt tgtgagggtt aaatgaaaaa tatgtaaaac tcatcaaaat tttaageaca 22800 aaataccaag tctcagcaga acacatacag taggagtcca gccattaaaa tttgaaacag 22860 gcagaggcaa aaatattttg tttagaagta cttgcatttt ctagctcttt ggctgagtgg 22920 acaacatttt ggtgttcttt ttcttattgt tattttttaa agcatacgta tatgttatgt 22980 agactctttt gtatgtatat ttaactcaca ataaatattt ataaatagaa agaaagatat 23040 cacatatatg tgcacataca ctggtaagcc ccaatacact gcctcatttc taaatgactc 23100 tagcatttgt aggacagtgc ttttaaacct ttcgcactca cgagatactc tggggagctt 23160 taaaatatat gctgatgctt gggctccact tccacatatt ctgatttaat tgattagggg 23220 ttcggctcag gcatcagtat ttgtaaataa aagcttccca gctgattcta atgtgaagtt 23280 aaaattgacg gatactgata ccgataaaag agtgagtgca atagacacaa aaca.aaatag 23340 gagatgtgtc tcaaCaatgg taagattgtt ttaaaaatta tagtacaatt aaaaaaataa 23400 tgtaatgcaa ttatttagaa aattcttaca attttgtatt ttgaaagcac catgtggata 23460 tcatggggaa aaccagaact ttgttggatt tccttaacaa agattttgca gcttggtatc 23520 atttccattt tgtagtattt tgatatccag gagatgaaag tgggaagtgg acaattgcct 23580 ataaggccat gctttgcacc agggtgtcca gatgagaggc aagtggaggg taaagtttag 23640 cctgcattct gttccctaag gtacatctcc tggtgagggg tggcttctaa tcaggggaag 23700 aagtcctatg tcttgtttcc taatgcacgc acaggttcca caagccctca ctttgttctc 23760 aagagcatag catttatttt ggtgcttaag actgctgaat gtcacaaaat aatttttccc 23820 agcacaaaca aatattcaaa atagttatgt gcacagcctg tttgagcacc aataagtttt 23880 ggctctgtgg cctgtttggg cactgacatg gtttggctct gtgtccctac ccaaatctta 23940 tcttgaattg tgatccccac atgtccaggg agcgacctga tgggaagtga ttggatcatg 24000 ggtgcagttt ctcccatgct gttctcatga cagagagtaa gttctcagga gagctgatgg 24060 tttaaaagtc tttggcagtt tccctttctc tccctctc'tc tcctgctgct atgtaagacg 24120 tgccttgttt cccctttgcc ttccgccatg attgttttaa gtttcctgag gccttcccag 24180 ccatgtggaa atatgagtca attaaacctc ttttctttat aaattaccaa gtctcaggca 24240 cctaaaccaa ttagaaggtg tgccattggc acacagcagg gtcctggtgt ctcccccacc 24300 atccagccac cactctgcca ggtgtgaatg tattaactat tgctggagtg aggtgaaagg 24360 aggcagtcct gcagaagggc cactgccact gggatggcac aggagggcgc tcgagagact 24420 caccttagtg actctatcaa ctggtgaaaa tatttagata ttttcacaat caatatggcc 24480 ttgcttgtat atacagggtg attataaact ctcttgccac ttatcacaga agtcgaagtt 24540 tcatcatttc ttttcctgta tttggtttat gtgcaacaat ataggtttat tttccagttc 24600 tggaaaataa tcatggttaa tactgactga gaactgatca tttcctttcc tctttatacc 24660 aaccaatggg gcaggtacca ttattaaagg gggaggaaat agaggttaaa taacatgccc 24720 ccaatcagta gtggggaaat gaaagaatca ggattcaaag ccaggtttgt ctgggtcttg 24780 ggaccagctc agcgtctgtg ctgtactgcc cgggactcct gctgccaatt ctttctacct 24840 cattatccct caaccctgca ccagcaaatc cacagggcgt gagatgaaga gaagagagga 24900 ccacaggcga gaaagtggca gcagcaagag aagatgtacc atgaggaagt tctgggaggt 24960 tctgcccgtc tttctgagaa caggggagag acagagctga gttacaactg cactgcttct 25020 tagcttctta gtttctcttc agaattagtg gcaaagaaga gttttcgaag tctttagcat 25080 gtattaaacg aagaattttg tttataagaa aaaattgtgg atcgttccca tgtttgtctg 25140 gaaaactctt gttaaacttt tgtgcagcta gtaaattaag tcactgttta ttctgtgtag 25200 tggaaagcat gcttaaactc taactcagga gacctgattc caggaatagt taagcctgag 25260 ttcattataa accacttaag ttatctgtgt cattattcta gtctgttaaa tggagggctt 25320 gagctggttt cttgtaaggt gggatttggc taagtgctct tggatacagt tgctcacctc 25380 tttgagcctc agctttcttt ttggtgatta tttaatcgca tgtgataaca aatacagggt 25440 gttttgtgta atgcctggaa tggcactgag attgtctcca ctacagtaag gaggtggttt 25500 agaaattagc taatttatta attaatggcc tatagagctt catgtaggtt ggtacttcaa 25560 agtcccattc atatggatca tgttcatatt tatatattca tcacttttat ttcaaaatct 25620 ctttttctgc tgagatcaac tgagatttct agaaagcagt cctattgtca gcactttgcc 25680 cagtacattt agtaagaact tgactagcat tgactcaaac agttctctta gaagggctgg 25740 caccttttgt ttcaattttt ttaaacatta actgcccata ataattgaag catgaatgca 25800 ctttctttgg attatgaaac aaatcaatca cagacatggg aattaggtgt gttctcttgc 25860 tttgttcaat atcatgcatt ttttttgtct gagctttcac tgtgtcccca gaactgtgct 25920 tggtgctaac agtggcccca cagagtacgg ctttatattc aaggagatga caatcttgtt 25980 ggaggaaaaa ccttatacat agaacaatta aggaacaatg caagggaaac acacattgca 26040 agttttgatt gtacctattt tatggctatg gtataaaaca aagcacctgt tgttcctgag 26100 agtgaaggga taagacaata actacaaaaa ggtgaaatga gttaattcaa aagggaacat 26160 tgagcatgtc attttcctat tgaagtgcta ttatttggta tcggcttcct ttggtggtgt 26220 tggtttttga ttccctctgc cccggactct gttgtttaaa gatgttcttt ctatgaggga 26280 tcttggatgc attttagcag tgtttccact gccctttgtt ttatattctt tgatgccatc 26340 ccaggtacat cacattcttg ctagatgagt aaaggtaagc atacttagaa gtcaaaaaga 26400 aaaggctgaa gtggtgactt agacttgaga aattatagat tccatggaga aaactgtgtt 26460 taaatcacag gacaggctga aaactcacat taccacctga tggaagtgaa ctctactttt 26520 ataggttact aggagaaggt cagccttctg taaaagcaga taaacaagga taccattacc 26580 agagtttcaa gtaatttaaa ttaagaactg aacattgata ctgatatgat ttctagttta 26640 ttatctgtga cagaacccag tagcttattg aaaaactatc atggaagaaa taactaagaa 26700 ataaccattt aaaatatatc aggatataat gaggatgaag ctttagataa cacaaaaagg 26760 caatttctct ttaggctgaa aaataccaga cacagatata tcgacagagt gacctgccag 26820 aatccctact ttccagccca tctgataaag gtcaacgatt gaacacccaa acacgttgat 26880 acacacagca cctgcctgat gaaaaccgtt aacagtgttt ttctcagccc agagtgcact 26940 gttttcttaa agttttgaaa acagtaatct aaatttccct attccatgag taaagttcta 27000 cttttttctt tttcacacta aacagcattc gatgttttag ctgaaagtca attaagaaaa 27060 atgtattgtg tctgtgtttt tcttgctttc ttttaagcac ctctaaaaga acttgttctt 27120 cttcctgaat tgagaacgag gtaggaatga aagactgaaa cggtaactct catcataatt 27180 ttattattat gacaagttgg gtttatgaag tatttttcca attactgcct ccaattgtta 27240 aataggtaga taacatgcca gctttaactg ccaaaaactt gtagtcctta aaatattttt 27300 tgtagtatta ctattagtat catgaacaga aatagcagtg ataaaaatca aatcaagttt 27360 g tgtgaacacc aaaattccat ttgcctgtgt gtggagggtg gtttaggatt ggtagaatta 27420 atattctttg caaacggctg cacctagcat ggtcgccttg aagccctggg agttctctaa 27480 ttttcctggc tcgagagact tccattaaac aagaaaatga ggtgggaaag gtcagaggat 27540 tcacagggta acaggaactg gacctgaacc tgagcttcat gcagtccgca gattttttaa 27600 gtggtttgtt ttttagaagt gtttgtcacg acctctaaat cattgttaat ttcatcgtta 27660 aactggcctc agagagattt tgttttaaag agacaatgca gttagaggat cgtgatctct 27720 gaactcagaa gcccagaact ccaaagctgg aagccaccag atcctagcaa gtgaagctct 27780 gtcaatctgg agccgcagat tttgaccacg tgtgcacagc ctcccatctg ggcaagccct 27840 tttctctcca ccactccact ccacagcttc tgtttctttt cttttctttt tttttcgctg 27900 aattcatcat ccctcccttt cccatgccga gacttaaaag gtttcaaata cttcaaaaat 27960 caatgatttc taaatcacct cagatgactt acatacaatg ttttattaca ttttcccaga 28020 gcagaatggc atattaagaa atggttttat tttcaactat attttctctt aggaatatag 28080 agacattatc tcaaattatt acctcgaaga gacagagaca agaattttgc accattctag 28140 ttctcaaatc attttaggta ttttatttca ttttgcctaa atctcttaga cggttatttc 28200 ctcgtatcac ttaaaaatta cattttactt gggcagggcc taaagaatta cattgaaaaa 28260 ctaagaaatt attgggaaaa aattccatta gcacaactat catgatcaga agaaattggc 28320 taatttcagt gtcttttctt tgggctcttc atagaaaatt gttgaaatgt gtctttcata 28380 agtaactggc attctgaaat tgatttaaag ctgtagaggg gaagaaaagc tacattggaa 28440 aaattaaatc ttaggtttgg agatatttta aaaaactcaa caagttccat gcccagagga 28500 gtgcagacac tgaccctctt tttcagctat tagaggtgag gaaaaaaagt tgtgtggttg 28560 gtgtggagag agagagacac acgcacttcc tttagccatc atcctactaa caccttcaca 28620 gtacattttc ttgctaataa tcccaaccat ttctacaggg aaaagtgcat gttcctgttc 28680 tctggtagaa gggccaccca aggaattcag gttcctggtg agtaagaatg acagaagtac 28740 ccacagctcc tgggccattg gtctcctcta tccagcctcc actgaccttg ctgggtccac 28800 tgcagttttc ttcttgattt aactctatga gctctctgtt ccttcaaagt actggagctt 28860 ctggccttag cacatctgtt tctgcagcca gagcaccttc actgagaagc agacctctac 28920 ttggctgggt tcttactcac Cctattggtg cctcttcccc atccccctcc.tcctcctcct 28980 cctcctcctc ctcctcctcc tcctcctcct cctccaggga acatatagtt ggcagcaagg 29040 gttgcagaac gaatgaaggc atgcatgaat gcacatgttg gaggacgaag gttctgtttt 29100 tactctcagt gcaccaaaac ccagcagagt taaatagtgg aaggttctta agtgctcaag 29160 tgagtagatg gaggtcataa ggcaagcaga gtgggaaggg ctagaaggag aagaaaggag 29220 aaagggaagg caggagagga gagaggtggg gaaaatggaa gggtagttga atgtcatagc 29280 aaattagagt tagaagagga gaattagtag aaaacagagc tagagtcaaa aacagagtga 29340 gacagacaga aattcataga tttgctgtct ggcccagatc tcattggata actagagcct 29400 cccttcttcc tttgggttga atggtcttta ctcattcttt atgtagacca tcctttactt 29460 ccacatggag tttgtatgtt cccatagaca ccaatacttt acatatcaga acacttgtca 29520 ttttattaaa aattgttttt tattagtttc ttttcccact agacagtcaa tgtcatggat 29580 gcaggactat agggtcttat Ctttttcatc acagtagccc tcgaacctag cacagtgaaa 29640 gttgcccata atacattact aaatatttgt tgaaagccta aagcactggg caaatcattt 29700 tgactttcca gacttcagga tccccatttg tgagggagct ggtctatttg atacctaaga 29760 ctcccctcca gcacaggtct tctataattt tgtatctaca tggcttcctg cctgatgcca 29820 ggaacttaag atttaactca agaagacagg gatgttgctt gcaaagaccc tgaagatctt 29880 gtcatccaaa ctgtgactgc Ctggagactg tttatacagg atcttgaact gggctaggag 29940 agtgcaatgg atggtagtcc Cagtccttgt ttgcagggag aggaagacag cgacaaagca 30000 tttctatgga aagaaggagt tgctcataag tctactatgg acatcaaaat atgctgatga 30060 gccacagcaa gttcattaca aagggatttc agatagaagc ctcctccttc agagtgagga 30120 ggaagaatca tccaggaaag agagaaatta tgtgtatttc atatattgct gtatgtacat 30180 attaatcagc cagcaaatat taagtgcatt catgggcaaa atgccaaaga gatgaagaga 30240 aaaatcagac tccaaggatc atataatctg attgtggaag aaaggcatat tcatcatgaa 30300 aatgtagcaa agatgttaag tgagtgtctt agtctattct gtgctgctat aaaaaatacc 30360 tgagactggg taatttacaa aacaatttat ttctcgtggt tctggtgact aggaagtcca 30420 agatcaaggc accagcagat tggttgtcgg atgaaagttc agtttctgct tcatgcatgg 30480 tgcctccaga ggacaggaac actgtgtcct catatggcag aaggtagaag ggcaaaagag 30540 gaaaaaccct ttttttttct ttttttttga gatagagtct cgctctgtca cccaggctgg 30600 agtgcagtgg ctcgatcttg gctcactgca agctccgcct cctgggttca tgccattctc 30660 ctgcctcagc ctcctgagta gctgggacta caggtgcccg cccgccacca tgcctggcta 30720 attttttgta ttttttagta gagacggggt ttcaccgtgt tagccaggat ggtcttgatc 30780 tcctgacctc gtgatctgcc tgccttggcc tcctaaagtg ttgggattac aggcgtgagc 30840 cactgcaccc ggccagaaaa actctatcag ttccctttat aagggcacct aatttcattc 30900 atgagggaag ggccctcatg cctaatcacc tcttaaagcc ctgcctgtta atactatcgc 30960 actggcaact cctgagtttt ggagggtgca cattcaaacc atagaagtgg gataagccag 31020 acact.tgggt ttcctatggg tcctctccat ttggttttcc tgttaaagat ttaaaagagc 31080 agaaacacta ctttattaga gaaagggcat ttgaacttgg cctttgaaca tggcaggatt 31240 tggccactta gagattgggg gtgccattcc aggtagagag aagagcaagg agaatggaga 31200 gcacttggca tttatgagaa tagtgagggt tcatgtcagg ttacatgaag ctgactcatg 31260 acaaattaat ttggaaaggc aggttgtgac caggctaaag gcatctggac tttgctctag 31320 aggaaatgtg aaaccattgc agatttctga gcaagctgtt ggccagagcc gtgcttcggg 31380 gcgactcaca acagtggtag gaagctcttt gggagctggg agagagagag agagagagag 31440 agagagagag agagagagag atgggaatcc aggggagaac agaacaagtg agaaagcagc 31500 tgcattgcgg cccaggctgg ggttgctggt ttctggaaag ggggtagtgt tgggggagtg 31560 gggagagggg ttaaatgtga gcttctgagg aagtacagga ggggtgaatt ccaccatgga 31620 gaaagtattc aataacaagc ctacagagaa gttgaggaag gtggaggagt aaaggacaat 31680 gatgtcctcg agaaacctgc tcatgaaacc tgctcaagta aacctgagga ggcaaaagcc 31740 tgattctaaa gtgtgagaga gcaacagcac aacctgggga ggtggggtat caagtccgaa 31800 ccaagggtga agggcaggga ctcaggtgct ggagtgtttc aatgtggatc ttggggttgg 31860 cacttgcttg ctgagttacc ttgggcaagt tactatgtgg tcttagtttc tgtaaaatgg 31920 agatcatgat gtaaccactt ggtagggctg ttggaaggat ggaaggagtt cagaaacaga 31980 aagctcccag cacagaacct ggcccgtaat aaacagtcta tgtgtttgct atcacgaggg 32040 tacttttaaa gaaatttagt gcagatgagt ataaatgaga cgtggagtga gtagcagaat 32100 caacacaagg tttttcttat aatggatgag agattgtaaa acacatttgc attggggaga 32160 aggacacatg ggagagggaa gaaatggaag acacggaaga tttgttggag gcaaatttag 32220 atccacgaca aaagtacggg agtaagtgca tggggaaaac ttagtattgg agggaggaaa 32280 aatggcctca ctttgcactt cgaataacat ctactttctt tacctggtcc tccaaaggcc 32340 accagggcca gcctctggcc tgtttcttta gccgtaactc ctccattctc ccccgctgcc 32400 atttgcacat cttgcatttt ctcagagctg ccaagatggt cgtggccggg ccttcctcta 32460 gctagcctat cttcctgcag agcccttccc tcagcttctt acttgg.ctga atctgttgtc 32520 ttacccagct ctcactttaa attccgaagt ccttccctga ccgtcaggtc caaagtagcc 32580 attcaacatt ccttacatta cccgtttttc catctttaca tggcattgat cttcacttgg 32640 tgattccttg tttatttatt tattggtgta ctgcctgcct ctcttcacca acgtataagc 32700 ttcattacag cagagacctc ttgcctggtc atcacagtat ccttggctcc tggcacatgg 32760 taggagccca gttaaacgtt tgttgaataa ataaatgaat acatccttct gtaatatgtg 32820 agaaaagtgg gaggcaagtc taggtgaaga aggcacttaa aaaaaaattt ttttttgaga 32880 cagtcttgct ctgtcaccca gactggagtg cagtggcacg atcttggctc actgcagcct 32940 ccacctcccg gaatcaagtg attctcatgg ctcagtctcc tgagtaactg ggattacagg 33000 cttgcgccgt tacacccagc taatttttgt tttcgttttt tttttttttt ttttttttga 33060 gacggagttt cactcttgtc gcccaggctg gagtgcagtg gcgcaatctc ggctcactcc 33120 aatatccgcc tcccgggttc aagcaattct cctgcctcag cttcccgagt agctgggatt 33180 acaggcctgc aacaccatac ctggataatt ttgtattctt agtagagatg gggtttcacc 33240 gtgttgacca ggctggtctc gaactcctgg ccccaagtga tctgcctgcc tcggcctcca 33300 aaagtgctgt gattataggc atgagccact gcacctggcC ggaagacaga ttttgaagtg 33360 gagaggagtt agaggaaatt gagtcatact taattttatc tgaaaatcat gaagtaaaat 33420 aatacataga aagtgaaggg gtgaacttct gggtgtatat attcaatgga aataaaatca 33480 gtgtcaaagg tttctctgca cccccatgtt cactgtagca ttatgcacaa tagtcaagat 33540 atggaatcga cctgagtgtc catcaacaga tgaatggata aagcaaatgt ggtatgtata 33600 cactgtggaa tactattcag ccttaaacaa gaagaaaatt ctatcacttg tgacaacgta 33660 gatgaacctg gaggacatcg tgctaagtga aaggagccag gcacagaaag acaaattgtg 33720 catgatctca cttacatgtg gaatccaaaa aagttgaact cgtagacaca gagaggagaa 33780 tggtgggttg ctggggagga aatggaatgc ggcagaaagg ggagatattg gtcaaagggc 33840 acaaagtttt agttagatag gaggagtaag ttctggagac ctattgtgca gcatggtgac 33900 tgtagttaat aatgatgcac tgtataccta aaaattgctg agcacagtag aacttaaaag 33960 ttctttttaa aatttttatt ttaggccagg tgtggtggct tatgcctgta atcccagcac 34020 tttgggaggc cgagatgggc agatcacctg aggtcaggag ttcaagatca gcctggccaa 34080 catagtgaaa cctcgtctct actaaaaata caaaaattgg ccaggcatgg tggtgggtgc 34140 ctgtaatccc agctacatgg gaggctgaga caggggaatt gctcgaaccc aggagatgga 34200 ggttgcagtg agcctagatc atggcactgc actccagcct gggtgacaga gtgagactct 34260 caaaaaatat tattttaatt tttataaata aagacaggtt ctccctatgt tgcccaggct 34320 ggtctcagac tcctgggctc aagcggtcct cccacctcgg cctcccaaag cgctgggatt 34380 gcaggcgtga gccactgcac ctggacaact tcgaagttct taccacaaaa aaatgataag 34440 tatgtgaggt ggtagatatg ttaattagct tgatttaatc atttcacatt gtatgtgtgt 34500 atcaaaatgc cacattgtac ccaaaaatat atgcggtttt tatttgtcaa ttaaaaaaaa 34560 gagaggggac tataggcaca caccaccatg ccaggctaat ttttttgtat ttgtattttt 34620 ttttttttga gacggagtct tgctctgttg cccaggctgg agtgcagtgg caccatcttg 34680 gctcactgta gcctctgcct tccaggttcc agcgattctc ctgcctcagc ctcctaggta 34740 gctgggatta caggcacatg ccaccgtgcc cagctaattt ttgtattgtt agcagagtcg 34800 gggtttcgcc atgttggcca ggctggtctc aaacttctga cctcaggtga tccacctgcc 34860 tcggcctccc aaagtgctgg gataacaggc gtgagccact gcgccaggtc atttttttgt 34920 atttttaata gagacgaggt ttcaccatgt tggccatcct ggtcttgaac tcctgacctc 34980 aggtgatcca cccacctcgg cctcccagag tgctgggatt acaggcgtga gccaccatgc 35040 ccgccctaaa ccttttttaa aaatatgttt taacattctt tgtgctcaaa ttcatgatca 35100 attttttttt tttttttttt tgagatggag tctcactctg tcacccaggc tggagtccag 35160 tagcacgatc tcggctcact gcaagctctg cctcccgggt tcacgccatt ctcctgcctc 35220 agcctcccga gtagctggga ctacaggccc ccgccaccac gcccagctat ttttttttgt 35280 atttttagta cagagtttca ccgtattagc cagaatggtc tcaatcttct gacctcgtga 35340 tccgcccgcc tcagcctcca aaagtgctgg gattacaggc atgagccacg gcgcccggct 35400 tcatgatcaa attttgtaaa tggttgatgg atagatgaaa aaaattctct aattgaaaga 35460 cataaggatc actgtatgat cagcaaatgt agataaatga atataatatt gattcacttc 35520 taaaagaaat atattgatgt ctcccactat aattaaattt taatcaagtt cttggacttc 35580 taaatagttt ttgccttata tatttggctg ttttcacgtt tggacgcttc actttattaa 35640 ttattgtatc tcctttgtaa gagtgtacca ttcatattac ataatctctc tatcttgttt 35700 ggtcaggttt tccacaacta atcaaaatgc cttggtatta acgtgactct ttttcaaagt 35760 agcgtggaca attttgctgt catcatgtag tcctgggtct ttgggtttta tctttctaag 35820 actgtagatc tcaatcttga gtttatcaat ataatctata aaaaattcca aattaagcca 35880 ggcatggtgg ctcatgcctg taatcccagc actttgggag gccgaggtgg gagaatcact 35940 taaggccaga aatttgagat cagcttgggc aacatagtga gaccctatct caacaacaaa 36000 ttaaaaaact gctggatgtg gtagcgtgca aatgtattgc cagctactca ggaggatgag 36060 gtgggaggat cacttgagcc caggaaattg aggttgcagt gagccatgat tatggcattg 36120 tactccaggt tggagtacaa actttcagct tgtcactgaa aaaaaggaca ctccatatca 36180 ctttttgcca ttttgtgtgt gtttacacat ctatctatcc acattgtgct taatttggac 36240 tttacaacca agtgattttt ctccattata aaagaaattg catcattttc ttttttttaa 36300 aattttactt taagttctgg gatacatgtg gggaatgtgc aggtttgtta cataggtata 36360 catgtgccat ggttgtttgc cgcacccaac aacctgtcat ctaggtttta agccttgcat 36420 gcattaggta tttgtcctaa tgctctccct ccccttgtcc cccaccaccc aacaggccct 36480 ggtgtgggat gttcccctcc ctgtgtccat gtgttctcat tgttcaactc ccatttatga 36540 gtgagaacat gcagtggttg gttttctgtt cctgtgttag tttgctgaga atgacggttt 36600 ccagcttcat tcatgtccct gcaaaggaca ggaactcatt cttttttatg gttgcatagt 36660 attccatggt gtatatgtgc cacatttcct ttatccagtc tatcattgat gggcatttgg 36720 gttggttcca agtctttgct gttgtaaata gtgctgcaat aaacatgcgt gtgcatgtat 36780 ctttatagaa taatttataa tccttttagt atatacccag taatgggatt gctgggtcaa 36840 atggtgtttc tggttctaga tccttgagga attgccacag agtcttccac aatggttgaa 36900 ctaatttaca ctcccaccaa cagtataaaa gtgttcctat ttctccatat catctccagc 36960 atctgttgtt tcctgacttt ttaatgatcg ccattctaac tggcgtgaga cggtatttca 37020 ttgtggtttt gatttgcaag gaaattgcat cattttccac tgaacctaag ttgcagttcc 37080 attctgccta agaccaccaa actcacctgc cccactgtta taccttctgg atgtcagtga 37140 aagttcatgg acaagagaat atttgtaaaa ctgcattgga tttctgttga gaaatgctat 37200 gttttcaaaa ggtgaaaaaa tgtagacatg tattttcaga ccttttattt ttcaatcata 37260 gcagtggaat tccttgatgg tttatgaaaa taaatgtttt tctcactcct aggccattta 37320 aaaatatata atatggagtt gaagttatta atagaaataa ctaagggtga gagagaatgg 37380 cacttgtact attgagtaca atattagaag caagtgctgt tatattgcca gactatataa 37440 aataacaagt gtttctagtg cactgagtga atgtatggtt tagaaaacat tactaattac 37500 gtgcgtattc attgatttat tcactaagta ctcactgagg gtctactgtg tacctgagtc 37560 tagccagagg cttgtggtgg agagctgagc aggtcaggtc caaacttatg ttcttgctat 37620 ttctttgggt gtgggtggtt ggatggttag ctcagtggtc tcggatgctg gaatttgttc 37680 taataagttt ctgagctgct tcataaacca agagggttag gggaactata gctgagtcat 37740 ctccttttcc tcctcttcct ccttcttccc ctcctcccca ccgtctgtta ctactattat 37800 aggaaatttg aactggaatg aaaaaaaaat cagtgttaca aaaatggtca cattttggaa 37860 taactctaca cttacatagt gtgttctagt gattagaaac aaaaaagcca tcattttagg 37920 ctagattttt cagaatgatc acaatgctac cactgttttg cacttacctt aaaaaataaa 37980 gtgcttcccc taagtttgat catttgcttg ttagcataat ttggccatta aacattaagc 38040 attgtttgag acccgtaagt caagccaggc tatggtgaaa caaaattagc aaaattcgaa 38100 ttgatgccgg aaactggtga agccgccata gatttaagga ccaatttgcc aggttaccgt 38160 ctgcaggttc tgctatggac caggtggggg cagcagagag ctggctgtgg tctccagaag 38220 ggaaggccag cacctgcctg cagattccaa ggcacaggtg gaggtggtaa tgagttacga 38280 ggtagagtca aggtttggac tgcatctgag aagggaggaa agcaagagag ttagaaacac 38340 cataaagatg agactgcaga gcaagccaga atggaagtag gacaaagaac tggtgtaagt 38400 caaagagcaa aggtgcaggg gtcacaggga ggagaggttg ggtgggcaga caggaggtgc 38460 ccgaaggagg ccagcctgct tcactagtgc cccttccagt accttcccaa ctcggtgagc 38520 caggtccata cttggttctg tttggtcttc caagggctat gaagggacag ggatgtgaga 38580 gacaccagcc atctctctct cctgagctat taataatgaa agttcctgtt ttatgaacaa 38640 atactgaaat gaatggagaa cactcaacaa tttggtagca atcagggtgt ggtagtgaag 38700 cattgcctgg gcttttcctt ctggttcctg caaaggacta aggtcctcag cagcctctgt 38760 ccatcttttc ggagattttt cacaatcacc aaggaaatat agttcagtgc tcctgtttac 38820 ttaatggtcg gctacgcttg aaatatcatg ctctccctgt gtggtcttgt cataattatt 38880 ttcaaatggt gctaattagc agacccaaca catgggcagc cttccttccc cagacttggc 38940 accctcactc gctgcattta gcacccctcg tgggaaggca tcctggagat cccttatctg 39000 caccctgtct ccatctaccc cttcccactc gcggaagctg cctcaccaga gaaactgcta 39060 tctgataaag aacatcaaca agtcctagca gatggctgct tatctcaaga cacatatatt 39120 caccaaaatc ctagcagaag aaaaagctgt gtttggatgg tcc~ctgagag ctaactataa 39180 catgttcaaa ctgtgtaacg cagtttggag atggagacag agtatggaga aagaaagaca 39240 aagggagaat acccaaaaga ggttttggaa aagaagaaaa atttgttctt tatacatata 39300 tatccataaa gatgagggtc tcactctgtc atccaggctg atctcaaact cctgaactga 39360 agggatcctc ctgcctcagc ctctccagta gctgcaacta caggtaccaa gctgagcaag 39420 aagaaaaatt tgagttaggc tttagaacat caaactaaga ctctgatttt cttgagaaag 39480 tggatagaaa atttgtggtt agagatgaca ccagtaacga taggtttcta ctcttagcta 39540 tgcctggggc agcttgaccg ccactttgtt gatcacatta atttacctct tcctgccttt 39600 atttcccttt ctgtaaaatg ggaattaatg tgcttgatga caatatctcc aaatgatttt 39660 taaggcaaga agagagaatg ttactgacag gcccatggtt ctctgggtga gagggtcact 39720 gtgaatgaat gtacacacag agccttttat tagtgaggct tagggtgtcc ggtttttctt 39780 ggcggagtaa acatccaccc agtagtattc actcagtttg cattgttctg tcgcattgtt 39840 tgtaaatctt ttgagtgatt acgcagcatt tgttccctat tgttcataat actctttaaa 39900 aagctgacta taggactcca gtccccactg gtttgttctt tgccacttgg caaaggttga 39960 ttgattttat ttttcctttg gcctgtgctt cttagtggct tgaaatgaac acggcttttt 40020 ttcctgttta cccctttgcc cacattgtca ataatagagt cgccctggga agcagagttt 40080 gcaaagggag gagggtttgg tataaatttg gaagattgta ttgtttcaga tttattttct 40140 caaatgctga aactgcttct caggggttat acttctttac tcatgaaaac tcacacattc 40200 tcttaggtgg tactgttaaa catgttacat ctgtaaaaaa attaataaaa cgtaatgaaa 40260 gaagcaggaa gcaagtgtat acacatggtg gcctgagcaa ttggctgtca tggtttggtt 40320 tgattatagg tgtgtgaggc acatgtatgg tgttgcgtgg aacagatgga tagaagtggt 40380 aaaaaagatg aatccaacct agtgcttttc aattctcctt gcacatgaga atcacctgga 40440 gaattaaaaa aaatacattg atgccgactg ggcacagtgg ctcatgccta taatcccagc 40500 actttgggaa gctgaggcag gtggatcact tgaggtcagg agtacgagac cagccgggcc 40560 aacatggtga aaccctgtct ctactaaaaa tacaaaaatt agccaggtgt ggtggtacac 40620 gcctataatc ccagctactt gggaggctga ggcaggcaaa tctcttgaac ccgggaggca 40680 gaggttgcac tcaaccgaga ttgtgccact gcactccagc ctgggcaaca aagcgagact 40740 ccatattaaa aaaaaaaaaa aaatttgatg cctgagtctc tccccaaaaa tcggaacatc 40800 tgtgggtgtg ggttcggcat ggatatttgt aaagtctccg tagctgcaaa gccaagttgg 40860 gaaccactga taaccaacag aaaaatgctt ctggaagtgt ttccggggaa ggagtcctag 40920 cctaggatca gacacctgtc tgggctcaat acaaaccact ggtttttttt ttttttttct 40980 gagatggagt cttactccgt tgcctgggct ggagtacagt ggcgcaatct cagctcactg 41040 caacctccac ctcctgggtt caagcgattc ccctgcctca gcctccagag cagctgggac 41100 cacaggtgca tgccaccact cctggctaat ttttgcattt ttagtagaga cagggtttca 41160 cactgttggc caggatggtc ttgatctcct gaccttgtga tccacccgcc tcggcctccc 41220 aaagtgctgg aattacaggc atgagccacc acacccagcc accactgggt tttataaact 41280 ctcagtaggc ctcctcttct ggcctggtcc catgctatct gacccttctt gacattcaaa 41340 aaaagtattt ttctttagtt gaactccagg aacatgaagt ggcattaaac agaagtatga 41400 attgccttta ttaaaattgc agtggtcttt ttcttaccaa aggtcctgac ccagttgtgg 41460 aaaaattgtt tcttgaatgt gggtgacaca tctgtttcat atctccaagg caatgcctca 41520 gagccaactc ctcccccgtg tgactcaagg atgccacgtg tcccacatgt ggcctgtttt 41580 cttctcttaa catggcctaa aaggccctta ttgaactccc gttagcttta tttatttatt 41640 tttgagatga attcttgctc cgtcacccag gctggtgtgt ggtggcgcaa tctcggctca 41700 ccgcaacctc cgtctcccag gttcgagcaa atctcctgcc tcagcctccc aagtagctgg 41760 gactacaggc gccttccacc acacccagct aatttttgta tttttagtag agacagggtt 41820 tcaccatgtc ggccaggcca ggctactctc aaattcccga cctcaggcga tctgcctgcc 41880 tcagcctccc aagagctctc cttagctttg aaagtaaaag ccaacccctt ttggctggcc 41940 catgaggccc cacacacctt agcattcctg ttacctctac cacctcctct cctaactctg 42000 ccctttgctg gtgccaattt gactgcacta gtgtcctttt tgtgactgca gctgtgcctg 42060 ggacactccg gtctttgctc ttgctactgt tctgagctgt ttatgtccag aatgctcttc 42120 cagcagttag ctacttgttt cctctgagtc ttcaggtagc tgctcaatat cagcttctca 42180 gtcaccctgt ctgatcacct ggcttataag tccagtccct acccttgtac tccccatctc 42240 taatccctgc ttaatcatcg ccttagcact gtcaccatct gactttcttt ctatatatat 42300 tttatttatt ggttagctcg gctgcttcaa tagataccat aaactgggtg gcttaaaatc 42360 agacatttat ttcccacagt tctggagggt ggaaatctga gatcagggtg ccagcatggt 42420 ctggttctgg tgagggcctc ttccaggtgc agacagccac cttgcttttt ttcttttctc 42480 tttttttttt ttttttttga gttggagtct cgctctgtca cccaggctgg aatgcagtgg 42540 tgccatctcg actcattgca atctccacct tccaggttca agtgagtctc ctgcctcagc 42600 ctcccgagta gctgggacta caggtgcaca ccaccacgcc cagctaattt ttatattttt 42660 agtagagacg gggttttgcc atgttggcca ggctggtctt gaactcctga cctcaagtga 42720 tccacctacc tcgacctccc aaaatgctgg ggggtcgtga gccaccacgc ccggccagat 42780 ggccaccttc tccctgtgtc ctcatgtagc agaaagagat caaagagctc ttttgtttct 42840 tttatgagag cactaatccc atcacaaggc cctgacctca caacctaatc accttccaaa 42900 ggccccactt cctgtcgcca tcatattggg ggtttggatt tcagcatatg gattttgggg 42960 ggacacaaat ctttcagtcc gtaatagttg gcctcctccc ttgttccact agaatataag 43020 cttcaggagg tcagggatct ttgtctcttt tgtttacagc tatgttccta acacctacaa 43080 caatgcctgg cacatggtag gtgctcagga gataattatt ggatgaaaaa aaatgaaccg 43140 gtctcctcct taacccctga aatcctgttc gagtcgctgg gtttgccttg ctccactggg 43200 atgaagtcct ggcttggtgt ccttgtctcc caggggtaga cgccccgtct ctgggaggac 43260 ttgaggctct cctggcctgc tgcttggcct ccttacctgt tctgcccact cagagccctg 43320 ccagctgcct gggatggctg ccgggcacgt ccttgttcct gctcagtcaa ccagttgggg 43380 ccttggtttg gaccttcact tattcttctg tgaacctacc tctctggtcc ccagctcgtc 43440 ttctgaaatt ttgatgtgtt ggtcaagccc agtcttgttt gcacatccct gtgaatgatt 43500 cttaattata tttctggctg gctctgtgca gtagaccata gaaataacga ttgtttctgg 43560 ggttgcgctg gagtgtgaac aggtggggaa aatgcctcag gacgattgga ccgaggagac 43620 tctgagatga ctgatgaaag cgacagcaag agctttccac gctcctgatg gcaggagggg 43680 acggcggtgc tgaggggtgg gggaggctca gggggagctg ttgagggtcc cgtgctcgtc 43740 aattctctag cttctcaggc gccttgatct tgtcatttag aagcctctgg aagcacaggg 43800 actgtggaaa ctggaagctg ttcttggact atttcgcaac acttttctcc aggaaaaaga 43860 aaaaataaaa aaaagcctca ttctcttcgt ccccaatgtt acataatcga acatttcccg 43920 tcctggttga aatgaatatc ctctttgcct gaataaataa tgcacagatt cttcgggaca 43980 ggctgcttcc tccaggccgc cgcttccaag ggatccggtc gctttgtggg tttagtggtg 44040 gcccacagct ggacgcgggg tccagcttgg ggtgggggag gcggtgcttt ctgccaaaat 44100 gtcttttaaa cgtctcaaca agtagcaaac aaggccccac ctgcgacagt tgtagtgatt 44160 ccggaagacc tgctcaccac gaggtgctgg ggtcgcttgc agctggcagt ctccggtggc 44220 tgcagtttaa ataaatctcc taagtgcaga atcaccacac agggcgcgga gaaactgaaa 44280 agttaaaaca tctggagtta caaacactca caggcctatc agctgtctga gtccccgtag 44340 gaaaactgtt tatcggatgc tttagtattc aaccttttat ttcctagcac aggttttttg 44400 attttcacat ggagtgtttg attattttcc catgcccaag tcactgtttt catagcctgt 44460 tgtttaatct gtgtgcacct ttgttttcac tctatttcct ttgctttcct atatgagaaa 44520 aataaatggc accattgaat tgcacggtag aggctgatgc tattccaggt gctcaggttg 44580 gctttcccat ctttcgtttc ctcctgtagg aagttttttt tggtataaag ggaaggacag 44640 aattggatct ccctggaggg agtttcatgt gtttcttatt ctgagctgcc gggagctggt 44700 tcacagcaca acttgataat tgctgagtag acctaaagtt ttgaaaatca agatctcttt 44760 ggagttttac aagtaaaatt tcaaaaatga gaaatgacta tcccaattta ttttagaaga 44820 aaggtgcagc ttgtaaactt tcatgaattt ggtaaaaggg ggctaatttg aaaaggaaaa 44880 ctaataggca atatttacaa attgtgaaaa atatcagaat agtatacgtg gaagagttat 44940 tagacattcc atttgcagtg gtaaacccat tcctttgatt ctgaatcttg tttaggtttc 45000 cttaagaatt tgatgaagtt ttcagactct tccttcagaa aaatgcacat attcttatgt 45060 acctaaatgt ttggatatga tttcagggga ttccaaatcc cctggggacc ccccatgtgg 45120 atgtttgtga tgcagaatgg ggtaagaaaa ccaggagagc ctacaaaaat tttatgaaat 45180 agaaatatga acatacagta ctgagccaaa atttagttgt gaggaaaatg atgttaaatg 45240 cattcatttt ctaaatgatg acagaaaatt agactcatat tactatccgg gtgaagcata 45300 ttttgtactt atggtgacct agtgatccaa atgcctgctc tttggtctta tcctcttatc 45360 ttccttatgt caatactggg ctgcttaaat atgtactgtc tccgatgtca ttatctagtt 45420 ttgttctttg ttttctatgt tttatctttt acttcttgtt cctatttcaa atactttgac 45480 ggtattggga cttggggaca tgtcctttcc agtacagatt ttggtattgt agagggaggt 45540 ttacaagccc aggtgggtgt acttggggac tgaggctgct cagtagccct gtaaatggtc 45600 agagtctgct gtttctgttg cttggagagc aaggtgaatg caggtctctt tggatattgg 45660 ggatgataga gggatgtgga ttggagagga acaggacttc ctgccccctc aatttaaatg 45720 gagattcatt tgatcaaaaa aaaaaaaaaa agcacatata ttcaccaggc cctgtgtgaa 45780 ctaccacctt ggatgtaggt ggagtaagcc atattcccag gtagcctgca atcaactgca 45840 atccattggt ggtaggaggc atgggagata cagaagtaac tcaggactag attgaattgg 45900 ccacttatct aagagtgtta tcaagtgttc tgtaaatgtg tgaattctga ttagttatca 45960 ggttgtcata catttttttt tctttttttt tttgagatag agtctcactc tgtcgcccag 46020 gctagagtgc agaggtgcta tctcaggtca ctgaaacctc cacctcccag gttcaagcaa 46080 ttctctcacc tcagcctcct aagtagctgg gattacaagc atgtgccacc acgtctggct 46140 attttttttt tttttttttt tgtagtttta gtagagatgg gatttcacca tgtggccagg 46200 ctggtttcga actcctgagc tcaactcatc agcccacccc tgcctcaaaa agtgctggga 46260 ttacaggcgt gagccaccac acctggctac gtgtcataca attcatttgt ctcccttcct 46320 agacacaagc tcctcaggat catatgtgtt cttccttgct ggcacagagg agatgcttca 46380 gtagtgcttg ttgctagagg agaagggtac aagtggcatg tggagagatt cagagaaggg 46440 tagaata'gga tgatgttggg ccatcaggaa ggtctccatg gagaaggtgt gctttgagat 46500 gaacactgag gaagtggtgg gatcacacct gacctagata gagaaggggc tgaaatccaa 46560 gcataggatg gtgggagcag ggaaagagag gttgactgaa gttgaaggct ggagaggttg 46620 gctggagacg ggtgatcagg gatatggtaa gtggtttgtg cttaatttaa tcttgcagta 46680 acacggtgag gatgattaat cgggaacata atctggtgaa ggtatgttgg acggattcga 46740 aatggtagag gctgtcggca accagaccaa ttaggacacc attgcaaaag tccagctgag 46800 agctgaagag gatctgactt gtgacaggga gtaggacaaa caagggatgg aggctggagg 46860 tattttggag atacagcctg cagttcttat aatccaaact tccccagaat atctcagccc 46920 aggaataaaa ataggatgaa aacaacaata acacaataat attttatctc tgctttctat 46980 caactgtttg caacctgcaa ttctttctgg ttcttccttc ctgcggggcc aggtgaacag 47040 gcaggtgctg aatatcactg ctgggctcac agaagccccc agtgctgggg ggccaaggct 47100 gcggctgctg gtgccaatca aaggcaccca taggcaggac cctcttccac taggtttcat 47160 tgcaaacggg aagcctcaag gcaggcgctt tccactgcta atcggtacct ggtacaggaa 47220 ttaaggcttc actttgtttg ttctgagggg tttacagaga tttttcttct gtaaagccac 47280 tgctcctttc actaaaattc cggagtttgt gcaagctggg aagttaaact tagcaacaga 47340 gtctctgctg tatttaaata tcatttttgt ctgacactgg cctccttttt tttttttttt 47400 tttttttttt tttttttttt gagacggagt ctcgctctgt cgcccaggcc ggactgcgga 47460 ctgcagtggc gcaatctcgg ctcactgcaa gctccgcttc ccgggttcac gccattctcc 47520 tgcctcagcc tcccgagtag ctgggactac aggcgcccgc caccgcgccc ggctaatttt 47580 ttgtattttt agtagagacg gggtttcacc ttgttagcca ggatggtctc gatctcctga 47640 cctcatgatc cacccgcctc ggcctcccaa agtgctggga ttacaggcgt gagccaccgc 47700 gcccggccac tggcctcttg tccttagagg aaatatgtga atttctcctt tctgctcaga 47760 agtacacctt agtagttgac aaacgtaaac tacccgaagg gccagccttc tctggatcct 47820 acttgagacc ttgcttcctg ctgagaagtg tgtgcatttg tgtatctggt gatcgagatg 47880 tctggaagag tggggagaag actgaggggc agatggggag taagaagggt cagaaagcta 47940 ttttggttca gcagtatcaa taatgttttt gctttaaatc attgtcttct tcaatgttgc 48000 ttcctttgtc actcctgtaa atatgatagc ccatttattc ttctaataga aaatttttca 48060 taattatttc ctcacataag atgtttatgg ctttatatat atgtgtgtat ataaagagga 48120 ggcacataat ttatgaaagc agattttaaa agcctttcta cagatgaaat gtaaagttca 48180 attgcatttt ttcttttgga gatgggtctt actctgtcac tcaggcaaaa gtgcagcggc 48240 acgaacgtgg ctcactgcaa cctcaaacag ttctcccacc acagcctcca ggtagctggg 48300 accacagaca tatgccatca catccagcta attaaaacaa tttttttttt tttttttttt 48360 ttttatttag agacggggtc tttctacatt gctcaggcta gtcttgaact tctaggctca 48420 aatttcttga tttcttccaa aagtattttc tgcttgacat tgtcatttga agaaagatag 48480 ttttaaaaaa atttgaagaa agagactatt tttatcccta taagcttgaa ctagggcttt 48540 catactgagt aaaagacagt atacagtgtc attgaggatg ggtcatatta tggctccgtt 48600 gtcgaacact ctagatctgg acactgaaaa gtggactact gatggatttt atgcctctgc 48660 tataaaaacc agcacctggg gctggacgca gtggctcacg cctgtaatcc cagcattttg 48720 ggaggccgag gcggccggat cacgaggtca gcagatcgag accatcccgg ctaacatggt 48780 gaaaccccgt ctctactaaa aatacaaaaa gaaattagcc gggcgtggcg gcgggcacct 48840 gtagtcccag ctactcagaa ggctgaggca ggagaatggc gtgaacccga gaggcggagc 48900 ttgcagtgag ccgagatggc gccactgcac tccagcctgg gtgacagagc aagactccat 48960 ctcaaaacca accaaccaac caaccaacca accaaccaac caaccaacca gcacctaggc 49020 cctggatgcg tgtttaaggt agctaaacac tcttaggtta cactattctg atgggaaaga 49080 ccaccagagg tgcccttgga tacctggtaa taagggctat agaagaaatt atggaaattc 49140 tacaaccctg tcagtgacta tagccaacca tgcggctata gccagaccct gcaatgtgct 49200 catgggtcca gagcccccta tgtgttatct cagctcatgg gctttctctg ttcctgcata 49260 acttcacttg ctaggaggct ttccttactt tggcctccat gccaccctga gacatgccct 49320 tgtcctttgc ggctcatgac ctcagtgcag gtttccaaat tcataccctc agagcttgaa 49380 tgattggtcc agttcatctt tttcacattt ggtcatgtcc taggtcactg gccagtctat 49440 agtttggctg atctttggtt g,ggtgtctca ccatggtcca gttagctgtg atctgaagtg 49500 ggcgtagggt ggggaggttg tatgaacttg agttggttgt tcaagtaaca ggagctgaga 49560 gcatggcaat tttccttaga acgatagtga gtgagtggtc aggtactgat tgatatctcc 49620 aggtaagcag agtttcaatt ttggttgctc ttcacgagga aagccagaag cccagagctg 49680 ttttgctaga gtgatagaac tgagatagtg ttttgagcag tgcccagtgg gaacatcttt 49740 gctgagaaga acgaataaag aattttggca attaactatg taactgagtc ccagactctg 49800 ctatcatctg gggtggcaga taaaaacaca cagtccaaat aaaaaacctg attttttatt 49860 ttagcctgaa tcacccagat cacagcagga ttttgcttga acaacccaga cagattgcat 49920 tcattttctg ctttactgtg cttgtggaag ccaggctgtt agtggtttct tctggaatgg 49980 gagaatgtat cacaaaaagg ttggaacctg aaaaatttct agaatttcat tgttctgtgc 50040 ttcttaataa acaatgtgac actatcaagg aatgtgtgtt gaccctcttt ttttaaaaaa 50100 ataaagatgt agtggatagc acaattttga tgaaatgtca ggctttattt ttcatttgaa 50160 aaatgtgtgt gagggaatca ggtgtaatgg catgatacat ctctttaaat tatacttacg 50220 agattaaatt tgtcctgtgt ttcaattaca ctgtattata ctgcttcat't taaattgttg 50280 ttttcttctt ttttaattgt tcaacctaat tgtctaatag tgatgaatgc aatttaatta 50340 gacacttttc cttgaaggat atatatatat tttccctata ataaagcaca gaatagttta 50400 aaaaatgtat tggtccaagc acacattttt atttttagtg atttcattag aacaataaac 50460 ttcaaaatga ttcataacaa cctttctgga ttgatttgat ggaactgttt ttacccattc 50520 actgataaac ctgtcaacag tttcctctca tgacacatac aaagcacgtg tgttcccata 50580 gtgtgtcctt gtgtgtaatt cccttgtgct ggttcagatt ctgaaatgga gctttaaaag 50640 catggaggct tgagttggtc catgtgagtt tgaaccctgg ccgcagctgg tatgggtact 50700 taatctcagt atcccacggt ttcctcaact gcaaaatggg tttttagtag tacttacctc 50760 actgggttgc tacaaagatg cttaaactag tatctgacat ctagtacagc cagccctcca 50820 tatctgtggg ttccacatct gaggattcaa tcaacctctg gttggataaa aaatatctag 50880 gaaaatagct gcatactagg ggaaaaaaaa aagaaaaaaa tatccaggaa aaaaattgtg 50940 ccagtactga acatgacttt gctcttttca ttctctaaac aatatagtat aacacatatt 51000 tacttcacat ttacattgtt ttaggtatta taagtaatct atggattatt taaagtatac 51060 aggaggatgt gcataggata tatgtaaata ctatgtgatt ttatatcagg gacttgtcct 51120 ggaaccaata tcccaaccct aatactaagg gatgactata tatgctcaga agacatttgt 51180 tgtcattttt attctcttgc actcaagaaa gcacatattg gaatgcaaac gaatatcaat 51240 aatagttttt taaaggataa ttaagttaat ttataattta catacaatat acattgtaca 51300 tttatatttt ctaatgaaaa ggaaataatc tataaccttc agttcagaat tcattagctg 51360 tcctattttg agaactgttg ctctatgtaa ttttcgtctc tgtattgatg tgtaaaactg 51420 gttttaacag gtagctaaat gctgtttgaa aagcatactt cacccattct ggcaactaaa 51480 ataattgaaa gatttaatta atttagaaaa aaatttcgct ttcaaaacac ttgaactgtt 51540 cagagcatgt tattcaataa tctacctttc accagaaatc ataataaaca caataaaaat 51600 gcttacagaa cccccacaat gcgttagtat acatgattat tccttgtgta agtgaatctt 51660 gttgagattt tatgctcagt Caattgcaat taaatacttt atatattaac taaaatgtgt 51720 cctgctctat gtattctgga gaagttactc tcagacaaat ttacatattt aaattatttt 51780 atgggctaac tgataagtat agagaagact gaagacagtt aagatcattg atttcaaaat 51840 gttttaaagt gttgattatt tattttggtt ttctttcttt cccatgccag ttttgagatg 51900 cacctcttac ttggcaggtg ttcctctgta ctgggtactg ggacagagag aaatacagta 51960 gcaagaggga gatcctgcct ccgccccatt ctaccattct cactgccaga aagccagcct 52020 tttttcaagg cttgtagaga agaaaaagag aaaatatttt taaaaattca tttaaagtac 52080 ctactgcata aaccacacca gacatgatga gatattaaaa tgtcaatatt attaaaaatt 52140 tttaatatga ttttacagcc cctttgctac tttaaaatgt ttatcttagt gttaaacaaa 52200 caatcaataa cctcataaac ttaaaaattg ctgcaggaaa taccggacag tttatggaag 52260 gatcatatga cagaaggaag ggctgaagag tgtgagaagc tagacctctg caggttaccg 52320 aagtcaagaa cctcattaat cggtaacaag aagtgcagag cgggcttttg agtccatgcc 52380 tgagtaagaa agtcccaaaa aacactcaca gaagatattt ccttgcccct gcttttagtt 52440 tagctgtagc taactttgga ttaacaaaat ttatgtgctg aatgatgttt tatttttttt 52500 tccaactcca catgcctgtc tagacttcaa gctttattac gaataaagag aaaatcggct 52560 ggatggcata aaaaatattt caggcagatt aacacatgat ttacctcttc ttgaacatcc 52620 atcttaatgg aagtgctaag aaagttagat tcgggcctgg cttggcaaaa gcaaggccac 52680 cccctcctct attttttcaa tgagattttc caatcctagt caaatggtgg tgctagttct 52740 ttatttttga gttactgcat ttcctaattt catggtcata acagcctcct gtctaccgac 52800 tcagaacgga ttttaccaaa actgaaaatg caggctccat gctcagaagc tctttaacag 52860 gctcgaaagg tccatgctcc tttctcctgc ccattctata gcataagaag acagtctctg 52920 agtgataatc ttctcttcaa gtaggtactc ctatttcttc tcaatttatt ttttcctttt 52980 tgatataatg tgctactgtt tacaagcata ttgtaacttc agagcttacc tctcatcttt 53040 aaaaaatgtt catttttttg tctttctgct ccaaggatat tttgcaaagt tactggcaag 53100 tattcctggg atgataaaat gtgaaatcta aacttggtac agtggaaatt cacttctaga 53160 ataatattta gctgaggcag agggcaatcc gactaccctt ttcttagtac agcacacaca 53220 ggctgcctgt ctgttccaga taacataaat gtattggatc tagcactagc taggagacac 53280 tgtattgttg aactgtgtta gaattttata agctcttaat tggacaaatc tcagagtagc 53340 atgaacacac tacctgtttt ctgaatcttt ggagccataa cttacgtgag tttgaactaa 53400 gcgatgtgaa taagccatta tttgtttcct aaaggcagtc aagttttctg aaaagctaca 53460 catttagcag caaaagaacg agcccctctg tcttgaaatg ggcctctgat tttaagcaag 53520 ctcttttgag tcctggtgtc ccattttctc agttcctttt tgcctcacaa tggcacatac 53580 ataatgactc caccacatat agcagtgggc tactcgggta atgatgtggc agtcacaaga 53640 cagggcagaa tactttcatt ttggttagag gaatgccaca tgtcttagga aatgctcgct 53700 gaactgtaag tttcatactt tgtcaagaag ataaccagta ttctctcaaa caagtccgta 53760 ggagccaaca tgattaaaag attttaaagc aatttactcg atagaagggt tgggcttaat 53820 caggacttgt gatcatggca atggttcctg cttaaaggtg cccagatttt taatgccttt 53880 ttgtgccaga tctaataggc ttacagagca actccatgta tatgaggttg ctgggaaact 53940 catctggttt tgaatgtggt atatacatat tttaatattg agagtaaact taggaagacc 54000 aggtagaagt atttgaactg aattctgaaa cgtacagaga acttaaattg gatgagaatg 54060 tttgagaacc atggatggtt cagggtccat ttgtataccg taaaatccct tcctatttct 54120 caaaagcaaa tgttttcttt gattttgtta atatcctgtg atgactgccc actaggcctt 54180 ggaatgcatg cagataatgc tgtagcagtt ggataaaata ctctagaatg tcagattttc 54240 aaggaaatta caaataaaaa caatgtaggt aaaagatacg acatgtagga aaaaagcaaa 54300 attttcctta agaatacaag cgtcgtattt acgagtagaa gatttttctt ggacatttgg 54360 agatacttgt ttcattttat tgcttgtgac agtcaaggaa aggaataaag ctcttgtgtc 54420 ttaaacttgc agcaagaaca aagtccaact atctttcgtt tctgataact ctgtgtcttt 54480 ttgttcaata ttgggtttag agctagctac catctacctt cccttcaaac actcactcta 54540 aacctccaca cagtgtaagt atttacatca ggcatctgct aaacattttc acatggttat 54600 ctgcaggtct tctctgagtc tgtctgcatt catttatcct agttgctcag aacaatcgct 54660 tgggctgacg gcctggtggt tgagtttact ttgctttgaa tagggaacag ctgtgctcca 54720 ggactgcaga ttggctattc ggaaaaaagg gcaaggaaga gagtgaccca gctctgggta 54780 gaccatccac actgtaaaaa aaaaagtcat agcatagtct tcatctatgg cagtggagat 54840 atggtcctgt ccatccacct ctatcaggag agggtctcca ttgttaatct ctgagaaaga 54900 ggctccatct gtgaaacctc ctgcatattg tctaatgaag tgctattttg aaaggcgtgc 54960 actgtacagg taagagtgat gatggtgtgc ctgccttctg ggcaacatct cagtagactt 55020 cctctgaatc attcccactg gacccaacag caaaagcgta cacatcattg agttttatcg 55080 ttctatctaa ggcgtataac atttttctct ctgaaagtct gtgattcttt cggagaatga 55140 aggggcttac cctatccatc tgcccctaaa aatattcgct ggaaagctga tacctctgct 55200 caggttttcc acagcagatc actcttcagt aagaattctg atattaagta gtttaattca 55260 gatccatgga aaatgactga gtaccagcca tagctcccac cactctctag ctgtgcaatt 55320 tagaggcaga ttatttaacc tcatgccttg aatttctcat ctgcaaagtg ggagtactaa 55380 cagtagctac cagatatatt atatggaata aatgacatta agccactcat taaactcctt 55440 gcagtttcca agcattagaa actcagtatg aggaagttat ttctattcta agcctagcac 55500 agtgcctggt actcaataca tgttggttga atcagtgaaa taattcaagg tcagcaccaa 55560 agctgctagg aatttatgaa catctgatca taactggaaa cttgatctaa aaagcaaagg 55620 gcagtcaatc caatcaactg agcgtaccat ctgttgaaat gctgctgctt ctgtaatgag 55680 tataaagtgt tggaagagaa agtccaggaa cctccatcat tcttccttcc atcattcctc 55740 tcctttgtga ctatctttgt gatgagaagg gtaacaaaaa aatcttgctg agatgagcgt 55800 gtcaaaaacg tgttacaaat gcttcacatt ccctttcaca tcaacagaag catgttgctt 55860 cattgttggg caatgtctta gtccatacac acatagagct ctatgctgat ttttttttga 55920 gatggagtct cgctctttca ccgaggctgg actgcagtgg tgctttctca gctcactgca 55980 acctccgcct cccaggttca agcaattctc ctgcctcagc ctccagagta gctgggatta 56040 caggcatgtg ccaccatggc cagctaattt tcatattttt agtagagacg gggttttacc 56100 atgttggcca ggctggtctc aaactcctga ccttaagtga tccactcgcc ttggcctccc 56160 aaactgctgg gattacagac gtcagccagc atacccggtt gtatgctgat gttctaattc 56220 aatgtgatac caaagacctg agatagtctc tcccactctg gccccataac atatgtccac 56280 gaggtggtaa taataacaac tatagtagca cctaaggttg gggcagctct tactttgtgc 56340 gatgcttttt atagtgttat tacgtgtgat tctcacagca accccaggtg gtgaacaacg 56400 ttatgattcc tgttgtacaa atgaggaaac taaggctttg caaagctagg taacatgccc 56460 aatattacac agcttcaaaa gtgacagccc taggacttga agataaactc atctaattcc 56520 aaagctcatg cttttagcca ttacttgaga cagtattaac ttttaaagtt tgtaatcaat 56580 atgaatttgg ccttgggaaa gcaggttaag catctggggt tgatgggaga taacattaca 56640 ccctctctta gcctcagcaa cttcatctgt aaaatgggaa taattacatc cgagtcacag 56700 aagttttgtg gctcttcatg aggattaaat aaagtaatgc atgtaaaaga gttttgtaca 56760 aagttcactt ttataaaatg caagttgtgg ccgggattgg tggttcacgc ctataatccc 56820 agcactttgg ggggctgagg tgggtggatc acctgaggtc aagagttcgt gaccagcctg 56880 gccaacacgg tgaaacccgt ctccactaaa aatacaaaaa ttagccagga gtggtggcat 56940 gggcctgtaa tctcagctac ttgggaggct gagactggag aatcgcttga acccgcgaag 57000 tggaggttgc agtgagccga gattgtgcca ttgaactccc acctgggcaa cagagtgaaa 57060 ctgcatctaa aaaaagacaa aaaaaaaaaa aagtaagttg ttatatgcaa tgcataaatt 57120 attacttagt tccatgtaaa atcttcccac taagtgaatg agggttcacc tggctgtagt 57180 gctatgagat agatatgagg ggcagattgg tttatgcttc tcaaaaacag aaagtgtgcc 57240 agggtggaag tgtgggtggg gagtctccgc ctcccagcca tgtggcaaag ctggaatgtg 57300 agtacagcag cagtatggat gcggttttga ggggatggtg gtaatcctct tctggcggca 57360 cccctccagt attgtgggat gctctctgat ttcttttgag aagacaagta gctaggagct 57420 tccctagcct ttctgttgta aaaaccatca agatccctgt tgaatgcata cctggagctt 57480 ggtttcccta agcacagact ttaataactt catttggttt tagtctccta tttaaagctg 57540 ccacccactc tcaatttttt gggttttcct actaagaatg gatataacat gggcagtctt 57600 ccagttctcc tttcttgctg ccttgaagac aacacacagg ccaatcacaa ggaggcagag 57660 acaggcccca acaagttgac aatcctagag agcttagtgt gagtagactt gctgaggttc 57720 ctgacttttg ctggaatagg agagtgccac tggctttttg acatttcttt ttccaactgt 57780 ttccttgtca aaatgaccag cagctcagct cccctaaaca tacctcctcc cctagattgg 57840 ttcagaggaa gccatcaagg tccttttgca aacggatgat ctgcattttt gagatccttc 57900 tttcctctgc tgttcataga aatggtctca ttggaataat tccttttgga atgttactaa 57960 ggacaccaag aaatcaacaa gaaaatttga gtgtatctga cagaagaaat ttggcttttg 58020 tactctaata attatttatt agaagcaata actggtcaga atttatttgc ttaaaaccat 58080 gtaaagaaag gtgcttaata aagataattg catcacatat agtaatccgt tttagtatct 58140 ttcaccttaa aactatgtga caaataaaga cacaaattgc tctttctttc taataagcaa 58200 ttttggaaat tccttattgg gaaattccaa attttacaaa atttacaaaa tttaaaattt 58260 tggaaatcgt acctgcatca agttttctga aagaatattt aaggattaag gttacttaga 58320 atccaatata tgcatagttt aatttaactc atattgttaa attctttttc taattttatt 58380 taagaaatga gtaatattga caagggcctt gctgtggttt attatggctt gtcctagagt 58440 ctctattccc agctagatta aaagtgcctc agggctgggc atggtggctc atgtctgtaa 58500 tcccaatatt ttgggaggct gaggccagtg aatcagttga ggccaggagt ttgagaccag 58560 cctggccaac atagtgaaat gctatctcta ctaaaaatac aaaaaattag ccggacatgg 58620 tggtgcatgc ctgtaatacc agctacttgg gaggctgagg caggagattc gcttgaacct 58680 gggaggcgga ggttgcagtg agctgagata acaccactgc actccagcct gggagacata 58740 gagagactcc atctcaaata aataaataaa taaataaata aataaataaa taaataaata 58800 aataagtagt gcctcgggtg catagatggg gttgttcagg tttctaccct gtgccttggg 58860 aagtgcctgg tacacactgg tgtttggcac ataattgtag agtgagattg agcctggcat 58920 gttccttgat ccctagacaa tctctatcag gtgtgatcag taaggcacat aatatgatat 58980 gtgactatta cagagtagca cactacaata tgtgcctaaa gccataacat caactaatac 59040 aattcagcag agtagaatgt gaagagtgct tgcagagtgc tgagcaccac ggtgaggaag 59100 tgtaaagaga agaaggtatt tctgggttgg tgcaggatgg ttagggatag ctttgggtag 59160 gaagcgactc ttgaccatga ccttaaagga caggtaatgt ttacatggga aaatacaagg 59220 aaaaggaggt gcataatgaa aggcatgggg gaggtggttt tgggaaacca tcagtctgac 59280 agaaacatag ttaagaaaag tgcaaggtgg tggtaggagg taatttagaa gtggagagtc 59340 agatgtccta ttaaggagct tgaactttat cttgtaggca ttaaagatgt tggtgtagga 59400 ttggaatgaa ataaagactt aaccttagaa aaatactgtg tggattgaag ttttcagatg 59460 aagagtgggt acatcaattc ggaggtgagg gaaatagttc aagggaggtg tcacaccatt 59520 aaaaaaaatc ttttaggact tgttttttgc aatcatgatt gtttatggtt gtaaagattt 59580 tgtttcagaa atgattggtg gagaaagaag tgtattgaga atcaaaagat gggactgtgc 59640 cactgatgtg cgcagtgaga agctgcaagc ttggtgcccc atcttcctaa ctgtaaaatg 59700 ggcataacaa ctaccagaca gccgtcaaaa cattatacta gtgatacaat gaagactaag 59760 tgatataatt ttgaaaggca gtatgcctta tatgtgtaaa atgctgttta cttggaatat 59820 ttttatagta aaattatttt ctttggacaa gatatagatg cagaagaaaa tgatcatatt 59880 tctgtgagat taggattcag accatttgaa ttttttatat ttattaaata ggtactttat 59940 gttgggtact gaataagtct tttattttta acacctcatt tccctcacaa aatggatggc 60000 atctttggac taggggaaat gtgtggtccc tgcagctgta gtattttatg attctatagc 60060 ttttgccttt agcaaacttc tcttagaata gtgatttaag ggctgggtgt ggtggcttac 60120 acctgtaatc ccagcacttt gggaggctga ggcaggcaga tcacctgagg tcaggcattc 60180 gagaccagcc tggccaacat ggtgaaaccc cttctttact aaaaataaaa aattagcccc 60240 gtgtggcagc atgcgcctgt agtcccagct actagggagg ctgaggcaag agaatcgctt 60300 gaaccgggag gtggaggttg cagtgagcca atattgtacc actgcactcc agcctgggcg 60360 acagaacatt taatgctggt ggctcacacc tgtaatccta gcactttggg aggccgaggt 60420 gggtgaattg cttttgctca ggagtttgag agcagtctgg gcaacatggc gaaaccccat 60480 ctctataaaa aatacaaaaa ttagccaggt gtggtggtgc acgcctgtag tcccagctac 60540 ttgagaggct gaggtgagag gatgacttaa gcccaggaga tccaggctgc agtgagctga 60600 catagcgcca ctgcacactg cattccagcc tgggcaacag agcaagatct tgtcttaaaa 60660 aaaaaaaaaa gtgacttaag atactgacag tataaacaca accaaaccca tgggcatctg 60720 tgctgggaat aaaagacaga catgttttca ttgcactgat ttaggattct gcttggaaag 60780 gaaaactgtg atctctcctg cagttgccta aaatgcttta cagttatggg aatggaaaag 60840 ctaaattctg tgatttgtgg gtagagggaa ggaggacgag tgtcctttct gatttcctct 60900 ttttccttct cttccaaggg gaaataaaag gctagagcaa caatttaaaa agaaaaggca 60960 aggagctact gggggtagag tcgggaggga aaaaggccaa acccactaaa taatttcact 61020 tcagttacag taaatctcaa atgatgatgc tacactcctg agaatgttcc atggtggaaa 61080 tgcctgggct ttgaattttg agcagcgaag taccaggaga gggtgacatg ataacattca 61140 acaggaagaa cactgctctt gtccgtttga cggtcctatt cctccggatc actcaatctt 61200 cacaaatcaa gcatatagct ccttcacatg tcatttgtga agggaaagac tcctaggaaa 61260 atgttttcta ggaaagggaa aaaagcagaa agcaaaggtc tcctgcttac tacagcattc 61320 gtgttgcata agcagtatta atgtagtgga gtattaatca cttagaacag ttcacgtatt 61380 attcctatct gggctctgca gggtcctttc ttcacttctt tctcgacatt tggattctgt 61440 ccattttact cctccctctg cacttggaat agaagtgaaa tttgttgtgc caattctctg 61500 cttgttagaa gccatggtaa tgtttagtag gggaaaagct ttgtactgct acatagaagg 61560 aggttttggg attatttaag actttacttt gttgatggga ttcctagaat ctactattac 61620 tgggtatact agacaagttt gtagatttac aaaagtgtgg ataacatggg ttgcacttga 61680 tttctttatc cagctctttg gttctaaatt tattgccaat tttatttatc aaaactattc 61740 tcagcggagt agtattttct gtggacacaa ggaaacatct gtgagcttaa accttagagg 61800 cagatagcca gttagaacat ttggcatatg aagcttagat agcagaagag aaaaattaac 61860 caaggcaacc ctcaaaaata ttgagaaggc aactaagaaa aatttcttat cactgacaga 61920 ctgcagtatt gagttcgtgt taatgaagaa atgtcagaat acataatgaa tcaataagga 61980 gtgtcatatt atggcaagtt tcaatgttgg tgtcaatctc gactgccagg atttatggta 62040 gattatataa tggaatcctg agttctgaga gatcaaagga aaatgctggc tctttcttca 62100 tccattctct taggtcaagc ctatgcagca agaagccaag ttgagaattg agcaattatc 62160 tgaagtgggc agtggagacg gccaaaccag ataatatata accagaaagt caccgtggag 62220 ggaaaatgtg gactgaaaat aggataaggg gtggaggtag aatgtcaagc catttggtca 62280 gtgtccacag tccaaattta ctgaataaca gccaattcct tacttcatgt ggttatttag 62340 agcgagacta gaggacaagt gaaaaaaaaa aaaaagagtt cattcctaag gttgttgcat 62400 gtttgtgcaa aatttcttca aatctctgtt attatctgtt acatgtctaa ttctgagtac 62460 ttccagagat tgcatttcat tactttagat tagtgggttg aagtggtgga ggaagcccat 62520 ataatctgac ttacatggag aaaacaagtc tttttttata gtttagttga gtgttatatt 62580 taattagcct ttttttccct ccataatgca tgcttatgaa ttttccactt aaaattctga 62640 ggctctgacc tttatcattt cctcatggag caatttctgt acctccttaa ctttagtaga 62700 acatagcaat gaaatataca agtcccctag aatagaattt gtgtgaattt ggttttctaa 62760 aaacacaact accaaagtct gaggaccagt gtggtatttt tttcttctat ttgatatgga 62820 aaccataaac tacttacttt gagcagatgt ttcccagtcc taaaatacag ctatcagtcc 62880 actggcccct ataaatctgt tggagtgcac ctgctaattc tgaagcctcc ctaatacaat 62940 gttaaacttt cctttttttt tttttttttt tgagaaaaaa attgtaaatt aggttcactg 63000 ttgcttggaa ttaggaaaaa ataaaaacac aggcatacta atatttcaga tggaaaaaca 63060 aagttaagaa ttcaacaagt ttttttcccc cactctatca tgtcttgcac atttaaagct 63120 gttatacaac attttaaaaa caaaccaaaa aaacccaggg cctttttcca acagcttagg 63180 ctgatgaccc acagtaaaaa agtgccccat gggatattct taaagcaccc caagggagtc 63240 tattgaaata acttaaagta aaaattcaga agaattttac atgtaattaa aaattaagaa 63300 cgttgtttta taagcaatag ggaaaagctg tattaagttg cattgctcaa tttctcactt 63360 gctctgtgca gtgactgctt tttcaaccat gtaacaacgt ctgaatcttc gtggtaaaat 63420 catacctatc acagccacag caggttttgt tctgctgctg cacatgtgat ttgagatact 63480 gtgggctggg agtttttttt ttttttaatt tctaatggca aaatggatct atagaaatgg 63540 aagtcatctg taatctcagc actttgagag gctgaggtgg gtggatcacc ggaggtcagg 63600 aattggagac cagcccagcc aacatggtga aacccatctc tactaaaaag ataaaaaatt 63660 agctggcggg cgtggtggtg tgcgcctgta gtcccagcta cttgggaggc tgaggcagaa 63720 gaaatgcttg aacccaggag gtggaggttg cagtgagccg agaccgcacc gttgcactcc 63780 aatctgggtg gcaagagcga cactacatct caaaaaaaaa aaaaaaagaa gaagaagaaa 63840 tggaagtcat cccgtgggcc tgagttggtg tagtggatta tggcctgtgc gtgaatgaag 63900 aaatacttgc caatggcatc agtggtaact agcttaagcc ctactcagct ttgtaaaata 63960 Ig atgtaatcaa ggaatttgat ctgaacaggt aagccaaaca ttgattcttc agtgcctatt 64020 gataagtgag actacttttc tttttaacag ccttatttca cttaagtggg gagtcaaact 64080 agctttaatt aaggaaatct gtagaaatca cccacatctc cctttccttc tctgttaaaa 64140 aaacaaaagg aagaagaaaa ctaggaagga gtaagcacaa agatctcttc acattctccg 64200 ggactgcggt accaaatatc agcacagcac ttcttgaaaa aggatgtaga ttttaatctg 64260 aactttgaac catcactgag gtatgtgtga acatactagt ttcctctttc tctctcctga 64320 ctttgtccgt aaattgataa gatctaattt ggtcatcagt ttggagaacg atttttcatt 64380 taatttcttt cattatcaag tgtgtattgt caggggctta gcagtacacc tactatctga 64440 tgggcactct acatgcgttg cttaggttga aattaaggat acaatctgtg cactaccacc 64500 atttaaaaaa atccccaagt ctactgtgtg ggtgaggttt ctttgcatag tatcagagag 64560 gttaaccaat ttatttacat actcaatagt ccactctaag tagggaaaat ccaagctttt 64620 tttttcttaa gaaagagctt ttcattcttt tttccctcag agctttacca tagtttccag 64680 caagtgaaaa catttcttga tttgaatttc acaacctcct tctcctcttg ccagttgtat 64740 ggtgctaata ggaagcaaaa tgtgagtgtg cttttaattt aaccctaaaa taggggaata 64800 tcttcagaat ttagacttta catattcagt gacctaccta aaaatagaga catagttcta 64860 accttctcaa tcattaagaa acatttctat tgaatttaat tattcaaaac aacaggtagc 64920 agtttcacaa gggaaaagac aagccatgcc acttgtattt gtggtcacca agtaattaca 64980 ttatttttta tttaaaatta agataaaata acttactgac tgtcaagtcc atttctcatt 65040 tcctccatag aacattttaa aactagttca caaacctctt gaaaagtcat gcctctagaa 65100 agtgcaaatt gtcaccattg cctagaagcc aacttgagag gctgtgtatc tatgcagcat 65160 tttgcaaatc cagtagcagg catgtgatat aaaagattaa atgcacccct gatttgtgaa 65220 tactatccta gccttcaaaa tgttcaaaag cacaggaagt ctgcactttt aaaaatataa 65280 cctgcccaaa ttgcaaggga ggggttgggt gtaggaccta ggtctgtctg ccaatcagct 65340 tgagatgcca gtggacgaaa cctgacttca ctaaaaacca gactggcaaa tattactcag 65400 cgtggtaatt tcctaattgt agaactggcc tgtttggtga agtatttcag gaaaattttt 65460 agctggacct tttctaccct actgcataag aaatgaaggt ttctaggcaa agactttact 65520 tagtgactaa ccaaaaatgg taaataaagt caccgcttcc aggcttagct actgtcctca 65580 aaacagctgc aggactggtc aggccagtct tctgaggctg gaggtgcatg tggaaccccc 65640 agagccttgt ctgcaagggc atggctgact gcaggctgtt tagaagcacc ccgccccgtg 65700 aaactcctct ggcttaggaa ttttaaacag ttctagttct aatctctctg ctcagagtct 65760 gagcagctgt gaatgagctc tcctgtgaat cacagaaatg ttaatgggtg agctgctcta 65820 gcttcttact tccaagtgga aggcgcctgt agctctttgc cagcttgatg cagtgagttt 65880 gattcctctt ttgggcagca gggagagata atgaaggagg gggaaaaaag attttaatat 65940 agaaacgagt cttccatctg gctgaggctg aacaaagtag aagaaccagt gcaagtcact 66000 gggggtatgg atctcatacc attttcagca tatacctccc tttctctctt tttgattcta 66060 cacatgtcag tccctgagta gtcttgcatt gctttcccca ctttgaagtc ttagatccaa 66120 aactctgatt cCagaaaggt ctaaggtgct gttgctgaga ttttgaaaga gacctgagac 66180 aaactgttgg gattctagac gctattgcgc atctgcctgg gtaactgtgt cacttaggtt 66240 gggtcaccag aataattctc ctccattttt gctgcctttc caatttataa acaacacaga 66300 acatcagtgg ttctcccata atcaaatata tttggctcta ttaatattaa aatttgatct 66360 tgagtagaaa gattctaagg gctaaaaaat aaaagatgta aaatgtatgt tgataggtta 66420 gtgtagaagc cacccatggg gttgccccca ctcccactct ttcatatttt ttttaagaaa 66480 caacactcaa gttacaaagt cgattctttt tttgttttgt tttatttgag actgagtctc 66540 actctgtcac cacccaggct ggagtgcagt ggtgcgatct tggctcactg caacctctgc 66600 ctccccggtt caagcgattt ttcctgcctc agcctcccaa gtagctggga ttacaggtgc 66660 ccgccaccac acccagctaa tttttgtatt tttagtagag atagagacag ggtttcacta 66720 tgtttcacca tgttgaccag gctggtcttg aactcctgac ctcaggtgat ccacccgcct 66780 cagcctccca aagtgttggg attacaggcg tgagccaacg cccctggcca caaaagtcaa 66840 ttcttaactt aaaacataaa agttccattt cttttatggt ctacttagtc atgtctggag 66900 tcagtgagct cagtcaagta gtttgcagaa tgacagttgt gatacctatt catttaccca 66960 ccagattatt ttctgctctc ccatggcact ttatagaaaa cagtttattc ggtcattaca 67020 acagctctag gagggggttt cattaaaccc actttctagg aaagcgaact aaggcttaga 67080 aaaatcaagt aatttgctaa agactaccca gctggcaatg atatgacagg acatgaactt 67140 aggacaataa ctcaaggtct tggtccctgt aaatatgggt ttccaggtta tgtatgcagg 67200 cagagggctt agatcctgaa gtttccttca gaaacttcag gatctaagcc ctctatatag 67260 gagcagtata aaaaggaagg atctcttccc atagggagct tgtcatccat tgaatgagga 67320 actcagactt ccactgtgaa atgatatttg tatccccatt gtgtgcgaga tatggggact 67380 ctgatcatca taagagcaca tttttcctgc ctataaatga tacttaatag agctgacaga 67440 ttatatatat ctggaacact tttacctagc agaaagacaa caggctagga ttagctgact 67500 aataatttta tttatcaagc tcaattccca tgctgacttc tcttcctcta gtgaaaatgt 67560 gtcatcagat ttggtcccaa actggagagg tgaaaggatc ctttgaccag tataatagcc 67620 atgccatcag ttttgctttc ctaattaagt tttatatggg ttaatgatct atatcattat 67680 tttgggggtt tctaaacaca tatgaactaa agatggaaaa gatgatggaa aagatggaaa 67740 gaagatgact cagatgtaca aaggcttgca gagagaagga taagccaaaa aattggtaaa 67800 tcacacatga ataatcaaga actgactaca atataataat atgtgtccat ttccactaat 67860 gaataagctg ccattatgtc tcttcattct ctatttagga caaattactt ctctgctgta 67920 tttccattct tacaggtacc tatctgctgt gatttttccc agttttgact ttatcttgtt 67980 gctttaatct tgtctctaag taagctgcaa cccagtaaat atattccagt attgtttatt 68040 gtaattcctg cctttatgtc aggtggcaag aggaagggac tggttttatt aatttatcag 68100 tctgggtgtg tgctaagagg atccttagta catgctttga ttatggtaga ttgagtctaa 68160 aagattcagc tggtttattc catttatttt ggtctgtcta cctaacaagg tcacacagtg 68220 gattatacta gttttctatg tgtcatattg ttggcctgac acataatagc aaataagagg 68280 taccagaatt ttctgtacag tctgaagctg tgtgtgtaca acaaccagtg aaaaatctta 68340 gttatttgga cttttgggtt ccatgatgcc agagaaagcc agacaccagc agctggaatc 68400 agctaagacc taagaccctg catgcacata tagatgttta cattttcttc ctattgcgac 68460 attcagccat tgctttggcg cctatttcaa taatatattg ctgctcatca aagtggaaat 68520 aatgttttgt tactagctga gtattgaaaa gctcttcata gttttgtgat tttgactcag 68580 ctccaacatg caggacccat ttcttcaact gactttggca acaggagaga ttgactagtg 68640 ggcttgaaag tgatccactg ctgtttctgt ttaactttct tcaatcctac tgggtcttgg 68700 gaagaagagg tagcgggcat ccttgtttgc ctcaaccagg aagcacaagg ccatcccagg 68760 gcggggcagg agagaggtgg gagggaagaa gcaggctcca cagggccatt gtttaccttg 68820 ttggcgggtc aggtcttctc agggtagggt tcctgggata agagcaaagc ttattggttt 68880 tccttgctgt gccacgagag cacaccagat cacccctgca gccttgcttg ccttcccaca 68940 gcctgccctt gcctagggtt tcagctgata tcctttctac tcaggaggag aaaccacaga 69000 acacatggag gaagtgtttc cagtgttaag actttagacc aaactataga aatctgttct 69060 acctggaaac ctgaagaaat aaatcatgac tgctactcag tagaagtaaa ataaaaaaac 69120 agctttactg gtttggaatc ataggaaggc tttctgtata gcctctctga gagctgccta 69180 ctggaaggat ttgtcctcaa aacgtgagga tttgtggtgt tccagggttt atatgacact 69240 ggcaggatag tttgctagag ggctgtgctt cgacctttat ccaagggatg taagccgtgt 69300 gtttaggtta gtgagcgtct agcacaaagc tctaatgttg aaggagcatt ggaggcaggc 69360 tgtgcctctg aacatgtaga attgactaga aatgccaatg ttcatttcaa aatgaaatca 69420 ttatctttct tatgcttcca tttaaaaatg attattacat ttttgcatat ggttgaatgc 69480 atattaattt tagcaagttt agaaaagaga taagcataaa gacatacata aaaatcactt 69540 attctcatca gctatagata atcactaaca atattttagg acattttctt ttcatctttt 69600 tcttagcaat atgtatgtgt gtgggtatac atatttaagt gtgtatatat gtaaattaaa 69660 aaacataaaa tattcccaca atataattaa taaaaatcta agatacaaca caatttccgt 69720 ttgtaatatg tagtgtatat atgtatatat acacattata tgtatatata aaatacatgc 69780 ataaaataga tacacaggta tatattttgc aaattgaaat gatgttgtat cttgtagatt 69840 ttatttaaca ttagaattta tttacactac ttaggtgttt taacaaggat tgtggtccac 69900 catttatttc cttaagagta attccaaaaa gtggaaatag aatgtcaagg ggcaaagttc 69960 tttataaagt ccttaaggag tattgccaga tatatttttt tttacttcct tttaaaacat 70020 gattgtattg tagaagttct ctttaaaaat tccaagcaaa aatttttcaa aatcccatct 70080 agaaatcaaa tcatgatttt atttggctat gcctcttgta gtccttgtct atgaacttat 70140 tctattatta aatataatag gtaatttcca ctaatttgac agatagaatt ttatttttta 70200 cattttctct tgttacagtg ccatgcatat ttctattatg ttacagagat cttgggggtc 70260 ttttttcctc cctttttcct ttctcttttc ttgttctgta ccccttcaca gaaaccaaca 70320 aatacaggtt gatgtgtatc ctctactctt ttctccatgc tcacacaacc caatatttac 70380 acatgcactg atttttcagg gcaatggtcc ttctttatca taaattgtgg gataaggtta 70440 tatacataat atatgtgtgt gtgtattact ctgaatcttg gttttgtcac tttgcaatat 70500 gtcttaaaga ttctgataaa tcttatagat ctatctcatt cttttcagta gttgcatgca 70560 gtataaattt actataactt attcaagtaa tctgctttca gctgatactc tgttgtgaac 70620 agttttcaac atataaaaaa gtgctgtcat tacaaatatt cttagatata tattcttagt 70680 aattgggggt tttattttta tggtgtaagt cccaggagta gaattgctgg tttaaaaggt 70740 atgtgtattt ttacatttct attggttttt tcatattatt ttctaaaaat attatagcaa 70800 atcacagtcc cactagtgat gttggagagt atcattttcc tcactagtac tcctatttcc 70860 ctgctttcta atctttcctg atctaatgag taagcattgt taattttctt tteattttct 70920 tgacttctaa tgagcttaag catcttttca cattattgtt gttcctttgg attttcttat 70980 ctgtcaatta ccaatggata tccttgacct atttctccct tatatggttg cttttctctt 71040 attaaaaatt tgtaaaagtt ctttgactat tacagatttt aaaaactggc agataaagtt 71100 gtatgattta ttgtgtataa tatgatattt tgagatatac atatatcttg atatatcaat 71160 atcgagatat atatatcaca cgttgtggaa tggctaaata tagctaatta acatgtgcgt 71220 tacctcatga agttatcatt tttgtggtga gaacacttaa aacctctcag catttttcaa 71280 2~

gaatataata tattgttatt aactatagtc accatgttgt acagtggatc tcttgcactt 71340 acttttccga tttaactgaa gttttgcatc ctttgaccaa tgtctttcca accctcacct 71400 cccacccctc tgcccgacac tgccccagcc ccggtaacca ccattctact ctctatgtct 71460 atgagatcaa cctttttaga ctctacatat aagtgagatc ttttggtatt tgtctttctg 71520 tgcctggctt atttcactta atataatgtg ctccaggttc atccgtgttg ttgcaaatga 71580 caatattttt ctcttttttt aaggctgaat ggtattccac tgtgaacata taccatattt 71640 tctttatcca ttcatctgtt gatggatact tagattgatt ctacatcttg gctattatga 71700 ataatgctgt aataaacatg ggagtgcaga tatggctttg acatactgaa tacattcgct 71760 ttgggcatat acccagtagt gggactgctg agtcattcgg taattgtatg attactgttt 71820 tccatagtgg ctgtactaac ataccttccc actaacagtg tgcaagggtt ctctttactc 71880 cgcatccttg ccaacacttt taatcttttg tccttttcat aatacccatt ctaacaggtg 71940 tgagatgata tctctttgtg gttttaattt ccattttctg atgattatag tgatgctgag 72000 cattttttct atagattttt tttttttggt cagtggattg ctaaatctta ccccgtcaat 72060 aatttctcct ttgattttac ttttataatt ttctgctaca aaattaaaaa ataattgtat 72120 gaaataaaat ttttcttcct atacgtattc tgaatttcct atcttacttc aaagagtctt 72180 cccacctcct attttctaaa attttcttct aaaaatttct atgttttatt ttattatttt 72240 taatatttag tattttttac atctggaatt tttttatata aaagtggaag gctcaactgc 72300 atctcttttt atataaagaa cgaattgttt aaacatcaca tgttaaataa tcttttctcc 72360 aataaattaa attatattag catcaattta aagatttttt ttctctaaac cactaatcta 72420 tttatttgtt gccaggcctg agacgtattg ctttgattat aatagctgta tagtctcttt 72480 taacatttgg caagtttgat gctccctact cattaatatt atttataatg ttagcctggt 72540 tttccttttt ttttttcagt ccaaatagga tttagtcaga agaaagatac gtggattaca 72600 tttttaaaat actgatcaaa atgaagatgc tccaaccgta taaatggcag atgaaataga 7260 ctttaaagta aaaaatattt atcacacaat atatcagaaa aatataacaa acccgaacca 72720 acaaacatca gcaacgtagc tccaaaatat tagcttgaaa catgaaattg ccaatagttg 72780 accacttttt gacctacaaa agcaacaatt tatataaaga aaaggtcaat aaaattatgg 72840 taaattgaat ttttttttat tattacactt taagttctgt gatacatgtg cagaacgtgc 72900 aggtttgtta cacaggtata cacatgccat ggtggtttgc tgcacccatc aacccgtcat 72960 ctacattaag tatttctcct attgctatcc ctcccctagc cccccaccct ctgacaggcc 73020 ccagtgtgtg atgttcccct ccctgtgtcc ttaggttccc acttatgagt gtggcgtttg 73080 gtttgatgtt cctgtgttag tttgctgaga atgatgattg ccagcttcat ccatgtccct 73140 acaaaggaca tgaactcatc ctttttaatg gctgcatagt attccatggt gtatatgtgc 73200 cacattttct taatccagtc tatcattgat gggcatttgg gttggttcca agtctttgct 73260 attgtgaata atgctgcaat aaacatacat gtgcatgtgt ctttatagta gaatgactta 73320 taatcctttg ggtatatacc cagtaatggg attgctgggt caaacggtat ttctagttct 73380 agatccttga gaaattgcca cactgtcttc cacaatggtt gaactaattt acactcccac 73440 caacagtgta aaagcattct tatttctcca catcctcccc agcatctgtt gtttcctgac 73500 tttttttttt tttttgagat ggagtctcac tctgttgccc aggctggagt gcagtggtgc 73560 aatCttggCt cactgcaagc tCCdCCtCCC gggttcatgc cattctcctg cttcagcctc 73620 ccaagtagct gggactacag gcgcccgcca tcatgcccag ctaatttttt gtatttttag 73680 tagagacggg gtttcactgt gttagccagg atggtctcga tcttctgacc tcgtgatcca 73740 cctgccttgg cctcccaaag tgctaggatt acaggcgtga gccaccgcac ctggcctgtt 73800 tccagacttt ttaatgatca ccattctaac tggtgtgaga tggtatctca ttgtggtttt 73860 gatttgcatt tctctaatga ccagtgatga tgagcttttt ttcatatgtt tgttggccgc 73920 ataaatgact tcctttgaga agtgtctgtt catatccttc acccactttt tgatggggtt 73980 gtttgttttc ttgtatattt gtttaagttc ttgtagatat tagccctttg tcagatggag 74040 agattacgaa atttttcccc cattctgtag gttgcctgtt catgctgatg atagtttctt 74100 ttgctatgca gaagctgttt agtttaatta gatcccattc gtcaattttg ccttttgttg 74160 ccattgcttt tggtgtttta gtcatgaagt ctttgtccat gcctgtgtcc taaatggtat 74220 tgcgttggtt ttcttctagg gtttttatgg tttcgggtct tacatttaag tttttaatct 74280 tgagttaatt tttgtataag gtgtaaggaa gggatccagt ttcagttttc tgcatatggc 74340 tcgcCagttt tcccatcacc atttattaaa tagggaatcc tttccccatt gcttgttttt 74400 gtcaggtttg ccaaagatca gatggttgta gatgtgtggc gttatttctg aggcctctgt 74460 tctgttccac tggtttatat atctgttttg gtaccagtac catgctgttt tggttaccgt 74520 agccttgtag tatagtttga agttaggtag catgatccct ccagctttct tttagcatag 74580 gattttcttg gctatacggg cttttttttg gttccatatg aaatttaaag tagttttttc 74640 taattctatg aagaaagtca atggtagcct gatggggata gcattgaatc tattaattac 74700 ttttggcagt gtggccattt tcatgatatt gattcttcct atccaagagc atcgaatgtt 74760 tttccatttc tttgtgtcct ctcttttttc cttgagcagt ggtttgtagt tctccttgaa 74820 gaggtccttc acttcccttg taagttgtct tcctacatgt tttattctct ttgtagcaat 74880 tgtgaatgga agtgcactca tgatttggct ctctgtttgt tattactgta taggaattct 74940 tgtgattttt gcacattgat tttgtatcct gagactttgc tgaagttgct tttcagatta 75000 aggagatttt gggttgagat gatggggttt tctaaatata caatcatgtc atctgcaaac 75060 agagacaatt taacttcctc tcttcctatt tgaataccct caatttcttt cttttgcctg 75120 attgccctgg ccagaacttc caatactatg ttgaatagga gtggtgaaag aggatatcct 75180 tgtctggtgc tggttttcaa agagaattct tccagctttt gcccattcag tatagtatta 75240 gctgtgggct tgtcatgaat atctcttatt attttgaggt atgttccatc aatgcctact 75300 ttgttgagag tttttagcac gaaggggtgt tgaattttat tgaaggcctt tttttcatct 75360 attgagataa tcatgtggtt tttgtcattg gttctgttta tgtgatggat tacatttact 75420 gatttgtgta tgttgaacca gccttgcatc ccagggatga agctgacttg attgtggtgg 75480 acaagctttt tgatgtgctg ctgggttcag tttgccagta ttttatcgag gatttttgca 75540 tcaatgttca tcagagatat tggcctgaaa ttttcttttt ttgttgtgtc tctgccaagt 75600 tttggtatca ggatgatgct ggcctcataa aatgagttag ggaggagtcc ctctttttct 75660 attgtttgga ataatttcag aaggaatggt accagctcct ctttgtacct ctggtagaat 75720 tcggatgtga atccatcttg tcctgggctt tttttggttg gtaggctatt aattcctgcc 75780 tcaatttcag aacttgttat tggtctgttc agggatttga cttcttcctg gtttagtctt 75840 gggagggcgt atgtgtccag gaatttatcc atttcttctg gattttctag tttatttgcg 75900 cagaggtgtt tatagtattc tctgatggta gtttgtattt ctgtgggatt gctggtgata 75960 ttccctttat catattttag tgtgtctatt tgatttttct cttttcttct ttattagtct 76020 ggctagcagt ctatctattt tgttaatctt ttcaaaaaac cagctcctgt gttcattgat 76080 ttttttttga agtttatttt gtgtctctgt ctccttcagt tctgctctga tcttagttat 76140 ttcttgtctt ctgctagctt ttgaatgtgt ttgctcttgc ttctctagtt cttttaattg 76200 tgatgttagg gtgtcagttt gagatctttc ctgctttctc ttgtgggcat ttagtgctat 76260 aaatttccct ctaaacactg ctttagctgt gtcccagaga ttctggtatg ttctgtcttt 76320 gttctcattg gtttcaaaga acttatttat ttctgcctta attctgtcat ttacacagta 76380 gtcattcagg agcaggttat tcactttcca tgtagttgtg cagttttgag tgagtttctt 76440 aatcctgagt tctaatttga ttgcagtgtg gtctgagaga ctgttaggat ttccattctt 76500 ttgcatttgc tgaggagtgt tttacttcta attatgtggt caattttaga ataagtgtga 76560 tgtggtgcta afiaagaatgt atgttctctt ggtttggggt ggagacttct atagatgtct 76620 attaggtctg cttggtccag aggtgagttc aagtcctgaa tatccttgtt aattttctgt 76680 ctcattgatc tgtttaatat tgacagtggg gtgttaaagt ctcccgctat tattgtgtga 76740 gaatctaagt ctctttgtag gtctctaaga acttgcttta tgaatctggg tgctgctgta 76800 ttgggtgcat atatatttag gatagttagc tcttctcgtt gcattgatac ctttaccatt 76860 atgtaatgcc cttctttgtc ttttttgatc tttgttggtt taaagtctgt tttatcagag 76920 actaggactg caacccctgc tttttttttg ctctccattt gcttggtaaa tcttccgcca 76980 ttcctttatt ttgagcctat gtgtatcttc tgcatgtgat atgggtctcc tgaatacagc 77040 acaccaatgg gtcttgactc tttatccagt ttgccagtct gtgtctttta actggggcat 77100 ttaacctgtt tacatttaag attaatattt ttatgtgtga atttgatcct gtcattatga 77160 tgctagctgg ttattttgcc cattagttga tgcagtttct cgtagtgttg atggtcttta 77220 caatttggta tgtttttgca gtggttggta ccagttttac ctttccatat ttagtgtttc 77280 ttttaggagc tcttgtaagg caggcctggt ggtgagaaaa tctctcagca tttgcttgtc 77340 tgtaaaggat cttatttctc tttcacttat gaagcttagt ttggctggat atgaaattct 77400 aggttaaaaa ttcttttctt taagaatgtt gaatattggc ccccactctc ttctggcttg 77460 caaggtttct gcagagtgat ccactgttag tctgatgggc ttccctttgt gggtaacacg 77520 acctttctct ctggctgcac ttaacatttt ttccttcatt tcaaccttgg tgaatctgac 77580 aattatgtgt cttggggttg ctcttctcga gagtatcttt gtgatgttct ctgtatttcc 77640 tgaatttgaa tgttggcctg tcttgctagg ttggggaagt tctcctggat aatatcctga 77700 aaagtgtttt ccaacttggt tccattcttt ctgtcacttt caggtacatc atcaaatata 77760 ggtttggtct tttcacatag tcccatattt cttggaggct ttgttcattc cttttcactc 77820 tttcttctct aatcttgtct tcatgcttta tttcaagttg atcttcaatc tctgatatcc 77880 tttcttttgc ttgattgatt cagctattga tacttgtgta tgcttcataa agttcttgtg 77940 ctgtgtttct cagctccatc aggtcattta tgttctgctc taaactggtt attgtagtta 78000 gcaattcctc tatccttttt tcaaggttct tagcttcctt gccttgggtt agaacatgct 78060 tcttcagctc agaggagttt gttattactc accttctgaa gccggcttct gtcaattcat 78120 caaactcatt ctccatccaa ttttgttccc ttgctggcaa ggagttgtga tcctttggag 78180 gagaagaggt gttttggttt ttggaattgt cagccttttt gtactggttt attctcatct 78240 tcatggattt atctaccttt ggtctttgat gttggtgacc tttggatggg gtttctgtgt 78300 ggatgtcctt tttgttgata ttaatgctat tcctttctgt ttgttagttt tccttctaac 78360 agtcaggccc ctctgctgca ggtctgctgg agtttgctgg aggtctactc caaaccctgt 78420 ttgtctgggt atggaggctg caaaacagca aagattgctg cctgttcctt cctctggaag 78480 ctttgtccca gaggggcacc caccagatgc cagccagagc tctcctgtat gaggtgtctg 78540 tcaacccctg ctgggaggtg tctcccagtc aggaggcaca ggagtctggg acccacttga 78600 gcaggtagtc tgtcccttag cagagctcaa atattgtgct gggggatccg tttctctctt 78660 cagagccagc aggcaggaat gtttaaatct gctgaagctg tgctcacagc cgccgcttcc 78720 cccaggtgct ctgtcccagg gagatgggag ttttatctat aagcccctga ctgggactgc 78780 tgcctttctt~tcagtgatgc ccttcccaga gagcaggaat ctagagagac agtctggcta 78840 cagcagcttt gctgagctgc gatgtgcttc acccagtttg aacttcctgg tggctttgtt 78900 tacactgtga caggaaaact gcctactcaa gcctcagtaa tggcggatgc ccctcccccc 78960 accaagcttg agcatcccag gtcaagttct gactgctgtg ctggcagcga gaatttcaag 79020 ctcatggatc ttagcttgct gggctctgtg ggggtgggat ccactgagct agaacattgg 79080 ctccctggct tcagccccct tttctgggga gtgaatggtt ctgtctcgct ggcattccag 79140 gagccactag ggtatgaaaa acaaaaactc ctgtagctag ttcggtgtct gaccaaatgg 79200 ctgcccagtt tttgcttgaa acccagggcc ctggaggcat aggcacccaa gggagtctcc 79260 tggtctgcac ccaagggaat ctcctggtct gtgtgttgca aagaccatgg gaaaagtgta 79320 gtatctgggc aggagtgcac cgttccttag ggcacagtcc ctcagggctt cccttggcta 79380 ggggagggag ttccctgacc ccttgggatt cccaggtgag gcgatgcccc accctgcttt 79440 ggctcaccct ctgtgggctg cacccactgt ctaaccagtc ccaatgagat gagctgggta 79500 cctcagttgg aaatgcagaa atcacctgtc ttctgcgtta atctcactgg gagctgcaga 79560 ctggagctgt tcctatttgg ccatcttgcc ccttggtctg gttttcaacc ttcagtcttc 79620 catatttatt ttgaatgaat tttctgcagc tttattaaaa aagaatagaa acaagtaaga 79680 gcaaagcata gttggtttat ggttgtaata aacttatatg tatgtcctaa tgatttgaca 79740 gctttgtgat agtatcttgt aatccaagta catgatatat ttctacatta tttagatctt 79800 gtgttaggtt tttccataag attaattttc ttcatattgg ctttcttgct atgtttcttt 79860 ttagcataaa gctaatccag gaaaaggaat aaaactggaa ggaaaaaatg tcttaaacca 79920 ttaatattgg ctgaccccgg tggtagggtt atgaatgaaa catttttgct tcttccactt 79980 ttttatattt tccaaattgt ccatattaat catgtatgat ttttataata ataaattaat 80040 gaaatttaag aattagataa ttgattgaat tggataattg aattaataaa atttaagaat 80100 gagaatagga ataattgtgc tttgaaaagt catatacaca agagttttag atggaattca 80160 atactaaatt tatatgctat actaaatcag taattctcaa ggagcaaagg tgctggggag 80220 tgtggaggag ctctttgttg gtgttgcaca tacaatttaa gacaacattt tccttattcc 80280 tcaccatttt aaccatatca cctaaccagc ctctggtagc actcaataga catctgatga 80340 atgaatgaat aagtgaatga aaacattgtg acaaaatggt ataacatttt gtatttgaaa 80400 aatatatgaa aatctattct tttcaaatat aaaatgggaa aataaaattc aataaaaata 80460 tcttggttgg tgagaataca caaaagatat accttcttgt ctatgaatta gtaataagaa 80520 attgtcttga ggaagtcaac tacatctgga aggttctctc tggacaagga gcataagtga 80580 aaaacagtgc actaaattat aaccaagagt tgcaacttac cattttaatg cttcagcaca 80640 ggcacagaga ctaacattta ctaagcaaac aagaagtctt ggcaagttaa ttaaataatg 80700 aattattttt ggtgaccaag gagttgggct tctcatttta aaaccatgca tgagattttt 80760 ccctttctac ccattactaa aatatcgtat tagtgtgaaa aattatacca ggactgggag 80820 aaaaagaaat cacatctgtc cttgacaatg ggctgaatga agaggtgaag gagtggtttt 80880 actatctaag tgactgaaaa ataggttatg gtgccccagc aaatccttgt tgtttgctga 80940 caattagtga tgtctgttta aatcatgcag ttttattagg ccttaaaaat atcttttagt 81000 tagtttcaat ttcatcaagg gaaaaggaga gcagagtaga gtggaactct gctcagagtc 81060 cggttggaag ccttcaactt tgttctccct gttttcagat gagtgtctat ttgtggctct 81120 accttttctc tttcttctct taccatgaca ctcccctctt ctctcttatc ttagctgttc 81180 ttttcttctt ccgcatatta ggcagtggga gaaacaccct aaatcagttg gaggtgagga 81240 aagaagaaaa gcacccctat cttggtagtt cctcattctt cccttcgtca cgtccatgtc 81300 atcaggctct gccttcatct gcgatctgga tacaatccaa tttatagcat ccattcaacc 81360 aaaaaatatt gagctcttac tatgtcaaag tagtgttctg gatgctcggc tccatcagta 81420 aacaacctag actaacatct tgccgttgtt tacatctcgt ggtgtagagt ttacaccttg 81480 tggtctagaa tttacatctt gtggtggagg caggcagatg attaacaata aacttaagta 81540 ggtctagatg tcaaaaggtg gtaaatgcta caggaaagag caaaggtcga gccaggtaag 81600 gcagattggc tgtgtgggtg tggaggaagg gtgcaagagt actgtcaaga taagccttcc 81660 tgagaacatc tgaaccagga catgaaggac atgaggaagt gagacagtcg ctatccaggg 81720 aaggaatttc caggcagaag aaagagccag tgttacacct taaggtggga gcatttctgc 81780 gagactggag aatcactgga gtccattgta cttggagccc agcaagggag aaaagattgg 81840 aggactcagg aggtgctggg gcaggtgaac tgctttttga ttggggagtt cagagggtaa 81900 tgctttggat aaatgtaact gaaaactttc tcagaagtgt tttcatacta tctctacaat 81960 tcattttcat gtgaaaactt aattggcgag cagattaata tggtgatctt cccttagatc 82020 actaggaaaa tcttgtttta tgaatttttc ttcccttttc attcactagc agagaaaaga 82080 gttgtaaagg agccgcagaa aattatagta ggtttctccc tactactgag ctactgagac 82140 tggaagatgc tcacgttagc aactgagtat atttatgtat ttccctttaa tgtttgaagg 82200 gtcaggaata tttgacttag gatactagtc tgtttagaaa catgattcaa ctagctacat 82260 gactaaacta gcagctgtca gagagaatgc aaggtcaaaa cttagtagtt gataataata 82320 aagaaagggc agagtgttat gaaatgttat gatctgggta atccattggc tcactttttt 82380 ttgtcagcct aaggttacaa gatgataaat taagttgcta atattctata tctaccattt 82440 acttttcctt tatcatgtgt ctggcaatat gctgagtgtt ctacctctag cattttattc 82500 aaactgtgca atctgcctgc tgaaacaagt atcattaact tcatttccta aggtgagaaa 82560 attgaggccc agagaggtta aataagttta ggatcatatg catagctggt aagtactata 82620 ctttaatatc cctttgcctt catgctttgc tctcactcca tgtttaaatg agcagagaga 82680 cttatcttta aacagatata tagcaagtag tattttccaa atgaattcca caagatgctc 82740 cattgcaaaa gttcaaataa gtttagaaaa atgctgcaaa ctttgtgctt cttaagagat 82800 ttacagtgca gattagcaca ttaaagcctt tgggaaattt gtattagaaa aacctttatt 82860 aacttattta ccccaatttt tccaagcttt ttgatcacga aactctttaa aaaacaacaa 82920 gtataattgc ttttatagta ttctttggga tacacttgga gaagtgttac atgaaaagat 82980 atgattggat aaatttaaat ataggaagaa ttatttaatc attttttgga ataaaacacc 83040 aattttaggt tgtctaacat acatttgagc caagacatga aggacatgag gacataattt 83100 acttttgtcc ttgtaaggac ataatgcact tttagtggtt tgcattgttt attttctttt 83160 gaatttcatg ttactttata aaggtaatta tttaatcaaa ccatttgtta agtgtcagac 83220 actatggtag gtgctggtaa tataaagaga atatgtaaac atcaacacaa catatctcct 83280 agtatgggct tacagtacag tgagagacac aaacaaataa tttgtaggat tgaaataagt 83340 cttacatttg ccacatcaat aaagcactat ggaaacaaag gcacaagaac ttaactttgt 83400 ccaatgaagt ctttcaggag gaggtgatat tggagctgaa ttttaaagga ttcatagatg 83460 tttatctgaa ggactaatgc aacatgttgg aggtgagtct tcaggaaaga ggcaacaaga 83520 accatttggg taacaagata ggacttggca agttagggga gtcgtaagtg gttcattacg 83580 gctagaatcc agggctctct ggagagattg ggagtacaca gatcactcaa gggtttggta 83640 tgaactgtca ggctactaag acagaaataa caaaaatcag atgtgggttt agaaagagca 83700 ttttggctgt aatacagaac atgatggata attgatggag cgagattcct ggggagatag 83760 aagaggatag ggccatgtaa gtacatctgc tggtatttta aaattagaaa aagaaatttt 83820 tggtagcaaa atcagaacag acccaccaac tctttgcaaa tcatgggact agaatgtttg 83880 gaaaggaaga tgctaacgat tttcattcat ttgtttatca tctatgttga gtgtcttctt 83940 gggcctaggc agcatgccca aagctgggag aataagatac tgtatgagat gctagaaaga 84000 tccccacctc tcctttccag aaattcagta tatcagacta attggtttac agttgtgacc 84060 atgggctttt ggcatagctg ttcaacaaga tttcattctt tttcaaaagc aattcattca 84120 tccaccaaat atttgttaaa caaatgtacg ttgcattgta gtgggtcctc agggagctgg 84180 atgtacatgc caaatatgtt tcattttatt tgaagcaagt ccacagacaa gtaaactagt 84240 caagtgatac caggcaaagt gatttgcttt ccaacagatg gtgacagatt aatttcctaa 84300 gagactgatc tgccccagag gcactgggaa agtcttgtta gggaggtaga ttatagtcag 84360 gaccttggaa aattgttggg aataattgag gtcaggaagg gtatacttag gtggatgggg 84420 acagcccctg agcgagggat cagtcctttt ggagtgagca cggaatattc aggtgcctgg 84480 gaggagtgaa ggcttgtgct tcagcactga ttgtgtgcac cgatggtgga acatacctgc 84540 ctgctcagaa gctttggctt ctctctgtag gtgagagaca gccattagaa gtccttgaac 84600 agggaagaaa cacggtgaaa gcaaggattt cattgagaag tccctcatgg aagaagaaga 84660 ttacattgga aagagcctgg cttagggagg gagcagtgac aatcagagaa aaaggcagat 84720 aggggcaaag atactggaag aagaggaaaa actgtccaaa gctttgtcct tgtgtgggaa 84780 agctggtgct gttggcgtga aagaggaagc aagaacatgt gcactgacac ggctgagagt 84840 ctccaaaacc attgtctttc cttaaaacag taatgatcac aagcagctta caactttgca 84900 tatagccact ggggcaaaat cctattaaaa gctaattcat tatctgagcc agtgcatgat 84960 aatgcgatgt aaaggcgtgt tgggaggcag atgatcagaa cttttaagca aactaaattt 85020 cataacattt attagaagcc tgtctctatt tccagaaaac gcctataaat gtggctgtaa 85080 tgtaggctac aggtcatcac actgaagtag aagcaatgta caatacctga aaggttagag 85140 aagttcaggg tctgggctta taaaaaactt cattattcat ttgaaagata ttgttttgta 85200 tcccccaatt gtggttttca atgtatataa aagcaggata tcatactttt tgatctatgg 85260 atttggaagg ggataatggt gtggtgggca ctgagcatat ggaattattt aattagagag 85320 atgcccttta gattactata aaatataacc agaatctcct gtttagactt ctaaaatcta 85380 cttctttgaa aacactacta aaatgaccac aaagacctgt cctcacaagt gggagctatt 85440 aatcaatagt gtgtacacat ggacatagag agcagaataa taaacactgg agactctgag 85500 gggtgggagg ggaagaggga ggtgagggat gagaaattac ttgatgggta caatctacac 85560 tattctggtg attgatttct ctctgtttcc tcttcagaaa ctgagaggaa atttagtttc 85620 ctcagtcacc actcctccaa actcctgcgt gggtgataca agacttgctg tcatcttgag 85680 gcagaagggg ttaggggatt tccccattga acctgatagc cttcatttca tttctgtctt 85740 ctcagggaaa catttacccc tctttactaa atggaagaag gtttgtaaac taggagggta 85800 tctatggatc tgattgctcc ttcaccactc tgctatatat ccatgtaaca aaacagcgct 85860 tgtggccgga tacagtggct cacacctgta atcccagcac tttgggaggc cgaggtggtc 85920 ggatcacctg aggtcaggag ttcgagacca gtctggccaa tacggtgaaa ccctgtctct 85980 actaaaaata caaaaattag ctgggcgtgg tggagcattc ctgtaatcct tagctactcg 86040 ggaggctgag gcaggagaat cgcttgaacc cgggaggccg aggttgcagt gagccgagat 86100 catgccactg cactccagcc tgggtgacag agcaagactc catcgtggga aaacaaacaa 86160 acaaaagaac agtgctggta ccccctaaat ctataaaatt ttctaaaaaa taaaatgaca 86220 ataaaggaat gaaaaaggca taggtacaag cagatgggat aggacactaa agcaagctgg 86280 agtaatactg gaattctaga aggtagaagg cttagcagag cagttcaggt ggaacccacg 86340 cctgtggggg caggggcatg agcagtgagc ccatctaact acgggacccc agataggctt 86400 gggatctaga agcgacagat gcctctaaag gctgggggta ggctgggact gaaagcagaa 86460 ttggtgtaaa gtctttaaaa ggagcaatta gatccacaaa taccttccta gtttctaatt 86520 attctaccgc ttaggagaga ggggtaaatg actccctgaa aacagagaaa tgaagtgaag 86580 gctatcatgt taaatgggga caacacctag cccctccttc ctcaagactg acaggaaagc 86640 tcctacaacc cacgaaggag tttggagggg tgttgtctga ggaaactaat aagtccaaga 86700 gaaagaacct tcagaaacgg acatttaaag gattaggtcc ccacccgatt accctagaga 86760 tacacataca gaaatgtgat tggacagtca aggatcaccg tatgatgctt ctaacctgaa 86820 caatagacac ctagtcacac acccaaacaa tcacacacac acatacacac acacacacac 86880 ttacttcact tctgctggag gaatttacaa gatgaagaac atcttgtatc tcttttgggc 86940 tactgtttgt gaaaggaatg gttgcatgtg ctcttggcta agccaactcc atcctgaaga 87000 cagatttttg tgaggtgggg gaaattcgcc cagcgttaat agtgttagtg gttgtcctgg 87060 gtccatcctg gtctgtattc ctgctttttc attttcagat ttaaatccag agttattaca 87120 gatggtaaca tctgatgcca atttatggat ctttttcata attcacctgc taggaatctc 87180 aggtaaccta taaaatatgc ttttatttgg tcattttaag agttacgttt ctgaatttca 87240 ggtattttat cctgtattgt cagcacatta aattagggaa cacattcatt gacattctat 87300 gtattttcct ttgtatttca aagtcagagt caagtattta aaaagataag atctttcatt 87360 ttgtgtgtga tcccacggaa tactttctac tgtagattta ttaaaattct cccacctctg 87420 gcctctgatt aaggaattgc agagtattct ctatgacaac tcattaaacc tattattccc 87480 cccagggcgg tttagtatat cactaaatat acttttagtg atatttgttg attggagcat 87540 agcttttgtg tttgccaaac atcattgatg tgttttgagg tcatgtggtt gtggggggca 87600 gggggtggtt gtaaatgtaa tagtgtccct ctagtattgt aatacttcaa ggacatagaa 87660 tttatttttt aaagtcttaa atctttttgt ttgtttttct aggaaatctt gagtctatgt 87720 tctttgattt gagactatag agtttcttgg taaagtctcc aattattggt tttctgagta 87780 aattcttgag aattggtgag gctcccagga ttattgtttg tcatcctgtt ctaattaata 87840 gagaaaaagc atataaatct tagatgtctt agatctggga ctattgcatc tacttggctt 87900 gagccaaagt gtcatctaat ttttatccag taattgtggc aacatattgc ttccaaaaca 87960 aatcacattt gtaaatgcat attgagcatt ttctacatga agggcattac cagggagtga 88020 aagaaatata aggtctgaat tttgtcctct gggagagaac attctagtgg gagagatgag 88080 ttgtgaaaag ttaaataaca aatatttata gatggtcaat ataggaactt gcaaatatta 88140 ttaagattaa ttacatgtga aaaattgtta ttgaattcaa ggcaaaagaa gtgaacttaa 88200 actgagttgc acaggaagaa ctcataaaaa ggtgagcttg tgttggatga gtggatttgg 88260 gcagataaag aaagacagga gcaaaaataa agaggtggaa aaccacaggg gtgtttgatg 88320 tatgcagggc acggaaatca gttgtatggt ggggggtacc ttcaacttaa aacctctgta 88380 ccagttaggg tgcttgagtt gtaagaagcc gaaaccaatt ctggctcatt taggcggaga 88440 aagaatttac aatccatgac taggatatat ccctccatct atttaatccc tgtttaactt 88500 ttctttattt tttatagttt gtatagtttt ctatgcagaa gacttgcaca tctttcgtta 88560 catttgttca taagtatttg acacttttat gttattatta aagattttct tttttttttt 88620 ttgagacgga gtctcactct gtcgcccagg ctggagtgca gtggcgcaat ctcggctcac 88680 tgcaagctcc gcctcccggg ttcacaccat tctcctgcct cagcctcccg agtagctggg 88740 actacaggcg cctgccacca cttccggcta atttttgtgt gtgtgttttt agtagagacg 88800 gggtttcacc gtattagcca ggatggtctt gatctcctga tttcgtgatc caaccgcctc 88860 ggcctcccaa agtgctggga ttacaggcgc gagccacagc gcccggtcaa gagttttaaa 88920 aaaatttcag tttctaactg ttgacactac atagaaatac aatagatttt tgcatattgt 88980 ccatgtatct gccaaacttg ctaatttaac ttattaatta taataatttt atctatggat 89040 tcttttggat tttccaaata tacaacatgc catatatgag tagtgacatt tttatttctt 89100 cttcctagct ccgtaacttt attctatttt cttgacctac tgcattgact aggatccttt 89160 actacaatgg gaagaaaaag tgatggtggg cattcttttc tcactcctga tcgcagggca 89220 gcatttaact tttcaccatt atgaatgatg tttgctctac agatttctgt agaaccattt 89280 atcagattca ggtagttaat cctaacttgg ctaacagcaa ttaaaaaaat gaagtaatat 89340 aaaaattaag aaattaatat gctgctaatg atatgttgtt gagtaacata aatataccaa 89400 tttttctata tctactgaga tgatcatatg aatattgttc tttatttact atggtgaatt 89460 gcattcatta attttctttt cttttttttt ttgagacaga gtcttgctct gtcacccagg 89520 ctggagtgca gtggtgcagt ctgagttcac tgtaaccttc acctcctggg tccaagtgat 89580 tctcctgcct cagtctcccg agtagctggg attataggtg tgcaccatca cgcccggcta 89640 atttttatat ttttagtaga gatggggttt ccccatgttg gccaggctga tcttgaactc 89700 ctgacgtcaa gtgatctgcc tgtctcggcc tcccaaagtg ctgggattac aggtgtgagc 89760 cactatgcct ggctggattc attaattttc aaatgcattt taaaatttct gaactaaatc 89820 caacttgatt ataatatact atcttttttt aagggaaaag tagatgaatc agactcagca 89880 aaaataggag ccagaatagc aactgatacg gtttgtctat attcccaccc aaatctcatc 89940 ttgaattgta atctccataa cccccacttg tctagggaga gatctggtgg gaggtgatgg 90000 gatcatggtg gcagtttccc tcttgctgtt ctcgtgatag tgaattctca tgagatctga 90060 tagttttata agggcctctt cccccttcgc tactcactct gtctctcacc tgccaccatg 90120 taagacgtgc cttggctact cctttgccat ctgccataat tatctgagat ctccccagcc 90180 atttggaact gtgagtcagt taaacctctt ttctttataa attacccagt ctcaggcagt 90240 tcttcatagc agcgtgaaaa ttaactaata cagcaaccat ggcaggggaa ggccaagcac 90300 cttgactgag tatatccaat gggagaggag atttttttct cacagccaaa tcaggacact 90360 cacagctttg tgtttaaaag cttgggctct ggactcaggc tgcctggttt gaagtgccag 90420 tcctccctct tatgtggcag gggaccttag ctctgagtat ccttctctat aaaggaggat 90480 gatgttctat ttacccaata agattctaag gattaaatga tacattaaag tgcacaggtc 90540 agatcactct cagcccatag aagtaattat aattccagct attattattg ttatttgtga 90600 atatgagaaa gaagaaagtt cactctgaca tcctgtaaat ggaaaattca actgaacatt 90660 gaaaaatgtt taaatctgtc tatcagaaag tttttcatca aaagaaaaca ctgagtagtc 90720 tagtgttgta gcttgatcca aaaccttgaa taattttcaa actcatttat gatatctgaa 90780 atattcaatg acttatacct ttgaattttt tggcagcttt gtggaggtat agctgacata 90840 taagaaactg cagatattca aaatgtacag ttttataagt tttgatgtat gtatacaacc 90900 attaaaccac taccacaata acataaggaa catacctgcc ccccatccac acatttgatg 90960 cgcctccttt taaaattctc tttcctaccc tccacccctc actgtttacc aaaaagaatg 91020 tctgatggtg ccctgggcag tcccactctg cctagaaaac accatagaaa aagacagcaa 91080 aagtgctttc tcactctgga gttctacttg attttaatgg acatagatta gtgtgaacca 91140 cataaaccag tactaaaata caggatttga tctagaaaag gtgatgctaa aaatgcatgt 91200 agataaaacc taattttttt cagacaccaa aatgaaaaat tattagtatg ccatgacaca 91260 tgaacacttt tatattccac aaccactgtg ctctcctttt ggtgttcatt tgcaatggat 91320 ggaatgttta tgtcccttcc aaatttgtat gttgaaatgc taactctcaa gatgatggta 91380 ttaggaggag aggctgttga gaggtgatta ggccatgagg gtggaaccct caagaaaagg 91440 attagtgccc tcagcaaaga ggcctaggag agctagcttg ctcttcccag catttaggga 91500 cacagcaaga gggtgccgtc tgtgaactag aaagcaggcc ctcaccagat accaaatttg 91560 ccaatgcctt ggccttggac ttcccagcct ccagaaccat gagaagtaaa tttctgttgt 91620 ttataagcca cctagtctat agtgtgtttt gttttagcag gctgaatgga ctaaggcatt 91680 atttattgtg agttctattc accatggagc atatgtggcc tatgtgtccc tggcactgag 91740 gcaggaactg agcagagtac gcaggggaat taataatgct tggacctatc tttaaaacac 91800 ctagagttta gtaggaatca actggaagta gtagggtaaa ggaaagtagg tgacttttgt 91860 tgagccctgc catgtgctag agactgtgct aaagtgattt acccacatta tcttatttga 91920 acctcatggc aagcctgtgt ggtaggtcct attcctctat ttacagatgg ggaaactgaa 91980 gctcagagac attaagtaat ttgcccaagg ttatagaatt atcagcaaca aagctgaaag 92040 tggcaaaggg ctctttctag tcaggaggaa ttaagagagc ttgggtgcag tggctcacat 92100 ctgtaatcct agcattttgg gaggccaagg tgggaggatc agttgagccc aggagtttga 92160 gatcagcctg ggcaacatag tgagacttcg tctgtacaga aaaaaagaaa aaagaaaaaa 92220 agagcttggg atcgtgcctc ctgtgactca gcatcacctt agtcttggtc tgcctccatt 92280 ctgaattgtg ccctaaagct agtcattgct tccttcttgg tttctgctac aagtttctgc 92340 ctctcccact ggcccatttc cggggttcct gttcctgtta tgcacatcaa tccctagatt 92400 tctgaggtcc cagagggtgg gcttctgctt agtccctgtt tgtcttccca gggctaggca 92460 tcctggtcct tagccctggc actggggctt ctctccctgc tgccttcgac cccccggggc 92520 tgaccatctg ctgctctatc cctgcatcct gccagctttc acatcccctg tctcgagctc 92580 tgcttgccct ttactcttga ggggaactcc ctgcctccac ctgttggact ggtctggtcc 92640 cttcactggt cctgcccttt ctgaccatct tctactctgg tctggcctcc cattttccag 92700 agctcatctg tctccatgtg tgtcctgctt ctcagagggt cttgagttcc agcttttcca 92760 ggtctggctg gtgccatggc caaaacctgt cccacctctc ccattcgagt tcctcactag 92820 ctcctttttg acagttcttt cttttttggg gtctagcttt ggccttgctg catgaatcat 92880 taacacataa atatgtgtct tcacaattaa ttgtttccat ttggtgtttg ctctagtcaa 92940 ggaggatgga gagggagcaa catcttgtac tgaatgtctt gacatcacta gaaactagac 93000 tgcttgtgct tcccgggcat gctgaggtct caagaataaa gagtatccct gaagacattc 93060 ttatcagtct tcccctggtg aaacattcat cctaattttt cct tttagct ttgagaccac 93120 ttttgcatga tttttaatat gtcattaaat taaatagata taattttctc tgtctagcca 93180 agctgctgtt agaagaatct ttgcatacaa ctaatcataa aaaagacatt cttcttgcct 93240 ctttggaaag gagcctgtgt cacctaagag tgaggagtac actcattctt ttgtgccttg 93300 ttcatctgct caggggatta ttgattagca gatagaatgt gggtgcaggc tgggcgcagt 93360 ggccatgtct gtaatccagc actttgggag gccgagatgg gtggattact tgaggtcagg 93420 agtttgagac cagcttgtcc aacatatagc gaaacccccg tgtctactaa aaatataaaa 93480 ttagctgggc gtggtggtgc atgcctgtag tcccagctac ttgggaagct gaggcaggag 93540 aatcgcttga actccggagg tggaggttgc agtgagctga gatcgcgcca ctgtacacca 93600 gcctaggtga cacagtgaga ctccatctct caagaaaaaa aaaaaagaaa tgtgggggca 93660 gataagtttt ttggggggtt tgcttttaat tctttggaag aactgggctt ggctggctgg 93720 gcacctagag caatgtggat atgggcagct gcttgtccca tggatgccag ggacagagtc 93780 acaagagaga tggttagaaa tggtgccagg ttctcgctgc ctcgtgccag gtcagctccc 93840 aacaaagcct ttgtacaaga cacatataaa ccctcagaga gtttatgcta acccagtgtc 93900 ttggccatgg cttactcatg atggaaaact gatcatattt tattctggcc acatggaata 93960 atagacatgt aatcactatg agatttgagt tgcagggtct ttggtttcta ataaccttta 94020 taattttctc cctcatcagg gagacagaat aaggtgttta cattactact ttcattttgc 94080 aagaataaga tcgaggttaa atctacagtt atgtcttgat agactgcagc agatttctgc 94140 atcagtagaa agtgctgttt tcccactaat cagaagaaga aagtgctagc cataatgata 94200 tgggtccact gtgaagtaga agacaagaaa cagagcagga cgtggaattg ggagcaatga 94260 gaaaaggtgc ctagagttgc tcttggcacc ctgcacctta gatcaccctt attaactgcc 94320 tgctgtttac tctgaaaatc tttctgtcat ttttgagaga taaaatctga aaatgcagtt 94380 tcttcaagtt agaaatgata atttttataa tagagtttaa gaaagccatg cttcttttct 94440 cttctatgag aacattttta tttcaattat aaaattgcca ctataaatag ccagtcaagt 94500 tcatagggca tgagtctcat caggcggata atttacatta tataaaaaaa gtatgagtta 94560 tttaactaaa ctaaaaaagc aaccagtatc ttgtggtcac atactgtatg tgatcccttt 94620 tacttgcaag gtaaaaagtg gtttcataac cttgccaata taattccaaa gaactaagga 94680 cttagtgatc gttgctaatt gaagttattc tgtttatgag caagcaacac tatttctttg 94740 atgaaaacca gaaaggattt acgtgtcggt aaatagggtt cctttatgtt tgtgtatgtg 94800 tgtgtgtttg tgtgtgtgtg tgtgtgtgtg tgattttatg aggtttgtct cattcttcta 94860 tgctagtgag tgttgattag ttggattgcc ttttcagttg acctattgct ttttaagagc 94920 atttttggat atagtccctt aacccagaat taacttctat gataaaaacg.ctttttgggt 94980 tgtcagagag gctaataggg gatatatggg cagggagttt tttaccagcc gtcagatctc 95040 atcttgcaga ctgtaactac tgtaaccatg ccccatgaag ggtagtacaa atcatgctat 95100 tgaaattgag agagatggct gccttttgga caggaagaga actagaagtt tttgactttg 95160 tgaatctaac ctctgcagct gcatccccca gggctgctgt ccacataccc tgtgctccac 95220 tccctctcat gatttctctc accaccgtca ttctttgttt ctcttgttcc tttatcctga 95280 agctctcaac ctatgaacct gtgaaaagcc cagataacag taacagatta catttattta 95340 tggaacattt attatagatg gtgtcatttt ctaagtactt taaccatatt gattccttta 95400 atctttgcaa tcatgcagtg aaacaggtga ttctcatact cccattttac agttgaggaa 95460 actgaggcac tgaggggtga aatgacttgt ctgggaccac atagtacgta gtcgagctag 95520 tatttggacc cagactgtct aactcaagag cccaagttat tggccaccat actctatagc 95580 acctctaacc cagatcaaat atacacagca ccatcacata gtgaaattaa tcagtgtcat 95640 gtccattccc tctgcttaat tgtatgtttc ttgtgatgag gaacataagc catctccaat 95700 gcctggggcc acacat'agca gatgccctat aactatcatc attgtcatcg tcattcttat 95760 cttcctcttc ctcctcatca taatctacta catataaggc aaatttgtgg gtcaggcaca 95820 cttcacataa attagattta attctcacaa caatcttacg aaatttgtgt tgtccccagt 95880 ttatagttga aaagaccttg catttaggaa gtggtaaaat gggaatttgc gaccctgcca 95940 tttggttcca gaatccttcc tcctaaccac tctataccat atgcttaatt aagaaaaaaa 96000 gaaacaaata ggtgagaaaa aacaatgatg tgaagaattt tgctgccccc agtattagga 96060 ttttttttct tcttcttgct agactctaga gggcccagat cctgtggagc tgtctctctg 96120 aagggaaggg tcaggctgtt caccctgatt ttcctggagt cacagataaa cttctgccct 96180 ccaccggatg cctctatctt acacaattat tttcctttac aaatttggtt gatgacatat 96240 tcacttggga taattgggtt gtccctccta ctttgaaact gtgagagagt tcactcaatt 96300 tccaactgcg tatgaagctc ttgagtcaga tttttttgcc tgtaacacct cagctcacac 96360 cagtgcagaa atggaagttt taattggctg taggattgaa gggttggttt ggtgcatggt 96420 ggtattttaa gaaaggcaaa gttttattta aatgaggaat attctccctg ggttttttga 96480 gttagctact gggccatgca acaggcctgg ctgcttgtga gaaaatgaga agggtgatca 96540 agtacaagaa gatgcagcca aagaagtgga cacaatccac gtcaagttcc atcttctttg 96600 tggcagccat gattctgaag gtcactgaag ggtatttttc cctgagctgt ttttggagcc 96660 tgtgccactg gcttcaaatg ctgggtcttg gccacctcag ttgctataaa atatggttat 96720 gagtgcttcc gCttCCCagC atCttgCtga CaaagCCCCa CttgtgCCtt gCtCagCCCC 96780 acttcattgc tcctgccctc tctggggaga gggactcacc acctgggtga caaggttagg 96840 gcttcttgct gcactttttg gcatcttgct gctccctgta taagggcaaa gctaaccttt 96900 tttttttctt ttcattgaga cagtctcgct ctgtcaccca ggctggagtg cagtggtgcg 96960 atctaggctc actgcagcct ctgcctcctg ggttcaagtg attctcctgc ctcagcctcc 97020 cgagtagctg ggattacagg cttaaaccac catgccaggc taatttttgt attttagtag 97080 agatggggtt tcatcatgtt ggccaggcta gtctggaact cctgacctca aggaatctgc 97140 ctgcctcggc cttccaaagt gctggcatta ctggcatgaa acaccaccac aactccgacc 97200 ttcaaaagat ttaaaaaaac gtgaaaactg aaatgaagac tagatactaa tgaaacattg 97260 gactcattat gactttgtta agtcgttttg attttattaa aatcaaacat taataaagca 97320 gactgtgaat aaataaatta tattctttgg ccatttcttc attagacaag gatgctatct 97380 ttcatggctg tgttattgtg gggatggctg atagctcata gaaccaatct aacatccaca 97440 gatttttttc atagttctgg actaggtttc ttagcataat gcgttgttgt ggtcctcatg 97500 gtctgtgatt ctctcttgcc aaccttgttt catcagtccc ctgttgtcct gcagattttt 97560 gaagcattgg aatcctagca acagatttct catttaagta ggatggtcct gatcactagt 97620 ctcccacaga gagttggtaa aaagttttgt tcttcttatc acccacagaa cttccctgaa 97680 acattttttg ttgtgcttgc tcagaaacca taagcttagt agaaaaatgc accttgcttg 97740 tgaatacaca atcaggtgtc caaatgagat ggtcccctcc tagaagataa tatttcggaa 97800 gtcttgcctc agtgggaggt acttggttgt ttgtttttgt tttttttttc atttcaatag 97860 ctttaggggt acaagtggct tttggttaca tgcatgaatt gtatagtgcc gaagtctggg 97920 attttagttt acccatcact cgagtagtgt actctgtacc cagggtcctt ggattttatg 97980 agcttgtttt tgtttgcttt tatgttaaga tatgaataat atgatatgct ttggctctgt 98040 gtccccactc aagtcttatc tcgaattgta atccccataa tctccacacg tcaagggagg 98100 gacctggtgg gaggtgattg gatcatgggg ggcaattccc ccatgctgtt gtcataatag 98160 tgagtgagtt atcatgacgt ctgatgtttt tataagtgtt tgaccattcc accttcacac 98220 gctgtcactc tcacctgccg ccatgtaaga catgcctctt ccccttctgc cataattgta 98280 agtttcctga ggccacccca gccatgtgga actgttgagt caattaaacc tctttccttt 98340 ataaattacc cagtctcggg tatttcttta tagcagtgtg aaaacagact agcatataat 98400 agaaggctct ttaccttttt aaacttggaa ggccaaatta atactgtcct ttccattata 98460 agaatcttca gattaaatat tttctcattc attgtccctc agatcatata catttgcttt 98520 ttaactctaa gttacatgta ataaactaaa caatgaaatt gtattgtgga tcataaactt 98580 gagttttaaa aagatttttc tcctaatttt aaaagtaata aaaacaataa atacttactg 98640 tattccaggt agtgttcctt tttttttttt tttttttttt tgagatggag tttcactctg 98700 tagcccaagc tgtagtgtgg tggcgtgatc ttggctcact gcaacctcca cttcccggcc 98760 tcaagcaatt ctcctgcctc agcctccttg gtagctggga ctacaggcac acgccaccaa 98820 gcctggctaa ttgttttgta ctttagtctc tactaaacag ggtttcccca tgttgcccag 98880 ggtggtctcg aactcctgag ctcaggtgat ccacccacct tggcctccta aagtgctggt 98940 attacaggcg tgagccaccg cgcctggccc aggtagtgtt ctaaggggca tgcttccatt 99000 aattcatgga attcacaata accctttgtt gcagtaacta ttattatccc cactttacag 99060 ataggaaaga ggtacagtga ctggggcaga gagaggctct gtgactttgt gcaagcagca 99120 atgtaggcat ttgaatttcg gcacgctggc tcctgagtcc attctcctaa aaattgtgct 99180 gtatttaatg cagagaattt caaaaacatg agataaaaaa taagagaaga atagcatcaa 99240 ttttcccatc attcaaaggt atctgttaaa aggaaacatt taaaacttag ctatatatac 99300 tttctgaatt tgtatgtaca tataaactta taaaaaatga aaatgctaat tgttttcaca 99360 cgtactgttt ggtagcctcc ctttggtatg ttcccagacc aaaaattatt attcttcccc 99420 atgatttttt tcagctacat aatgttctat tttatggaag tacatcattt ccataattga 99480 atttcttatt aagacatatt atttgcaaat ctgcaacaaa tgtgcatgtt atttttagac 99540 gtatgtttgg ttatttcttt acgatagagt cctagaatca ccggctgagg caagggagat 99600 gcacatttga agcctcttta ttcatcttgc caattgtcct caagaaacat tgtttcaagt 99660 cccattaatg caagcaatga acaaaagttt ttatttttct ggatactgga tgcctctggc 99720 ttatttttct ttctattctg tttctttaat agacaaaaca aaagaaaaag tttctcatat 99780 caatgtgcat ttttttgcga tttgcaaaaa gttcaaaaat taacaaaatt tatctattcc 99840 ttgagtaaat gtatgtggtt caagatgtca aagaaacaaa gtattgtgca gtggaaaatg 99900 agtgcacctt cagcacctgc ctcccagaca tccatcagta accctctcag gaggcgacca 99960 ctgttaccat ttccttgact agccttccta gaataattaa gcatatacaa gcaaagaagg 100020 gtataagggt acacacacaa ataagcatac tacatatatt gtttttgccc ttgtgttttt 100080 cacctaacaa tatattttgg aaattattcc atattaataa tcaaataatg atattattat 100140 ttttgatgcc tacctactat tgcattataa gggaattata agggaagggt tgcaaatatt 100200 aacatctgtt aattagcttt ggatatttct tcttttgcaa atacttgcca gtgttctttg 100260 agcag~tctta gaattgtggt tttaatgttt tgttattgat gatgatttgt aagtctttgc 100320 tgtgaaataa gatgaccgac cattggtatt aaatgatgta aattttattt cacatttcat 100380 tgttgtcttt tcaattttgt tttctggtgg taggcttatt ttgttgttgt ttgttttgtt 100440 ttggtgtggc tatggaagtc tttttgggca atcaaatcta tcaatcattt tgatttgatt 100500 tgtgaatgat tttcctggat tttcctggat gatcagaggc tagtgttgtc tctttgttta 100560 cttagattct tttgacaagg gctttggcta ttgtgaatag tgctagtatg aacatggatg 100620 tgcaaatatc tcttcgagac cctgctttca attattttgg gtatatgtcc agaagtggaa 100680 ttgttggatc atatggtagt tctattttta aatttctcag aaactgccat actctttctc 100740 ataacagctg caccatttca caatcccacc aacagtgcac aaggattcca atttctccac 100800 accctcacca atgctcgcta ttttttgggt gttatttttg acagtagccc atcctaatag 100860 gcatgaggat caattgttct gaacataaca tttctgtcag ggtttttaaa aagtttccaa 100920 aatgacatcc ctccagctcc acatattcta taagagcaat atcataaatt agaagctgtc 100980 tatgaaaact tgtagttgga agatgtttgt tttctgtggg ttagttacat ttcacatatg 101040 cctcatttgt gaaataattt ctaatgtgca tgagctggaa ttcaatgcct gtagtcaaag 101100 taggcttaat tttgaattgg agttgattaa aatgaaatga ctaattagct ttatattgac 101160 ttgggagttg tcccctaagg aaggcattgg gacaatgttg atactctgat tacctctatt 101220 cctacctctg ttagcttaaa atattagtac tgcattcagc acccagcaag aatttctagt 101280 atctgaaggc agcaggatac ttgcagccat tttcaaacca tggaatatgg atgattggta 101340 gagacttctc tatcttgagt cccttctaat atttcatgct ttatcttatt cataagaagg 101400 ggcttcacta agtaggactg tcttgtcttg accactaggg gatcaaggtt aagtttctta 101460 gtcttggtaa ttgtcagatt tctagtcttt aaaataggga taatgtcatg gagtacctat 101520 gtcagagttg caaggaccaa acgagctaag gcatgaaaag catttagcaa ggtccctggc 101580 aagtaagggc taaataaaca tggtgtttct tgatgttatg tcttgtaacc attgtaataa 101640 tctcagtcaa tcagatttat atcagggtac aggttctttt ctggggaatt tttctagcac 101700 caggtatttt ctgtttatct tctatgtgga tggcttttcc ttatttcttc tcattgacac 101760 caagggattc caagtcccat tctgattgtg tattgtcaaa gtgaaaaaca gctgtggcca 101820 gcagagccat gactataacc attttcaaag tcctgtgtgt aacaaacctc aaggattgat 101880 ttttcactaa gcctttctcc ttgactgtca aatgacaagc aagggtatgc agatgataac 101940 cccgaacccc tttgatccca tttccaaaat atggccatgc catagatgaa ggcaaattgt 102000 tttatttcat cttgttggcc ccattgtgta tgctctaagt tcattttgga cttctgtctt 102060 tactgatagc aatttctatt tctttcagac agaagcccct tctttcatac cttttagttg 102120 catttcttag cattctggcc gcagtcttct actacatggt ccttatttgt catctcttcc 102180 ctttcttcct ctctccatcc cttttttcca ttattcatcc agcaatattt attgagaacc 102240 aaccatgtga ggggcagcac actggacatt gtcttggctt cctctgtcac tgcagattta 102300 atggatcact ttagacttat gcctgtatta ttgccagggt tcccctatgt atattgaaac 102360 aaatattctc tggggatctt tccattttct gccactgatt ttttctttct tttctttttt 102420 tttgagatag agtcttacta gctctgtcac ccagcctgga gtgcagtggt gcgacctctg 102480 ctcactgcaa cctatgcttc ctgggttcaa gtgattcttt tgcctcagcc tcctgagtag 102540 ctgggattac aggagcgcac caccatgcct ggctaatttt tttttttttt tttttttttt 102600 gtatttttag tatatacagg tttttctcat gttggccagg ctggtctcaa actcctgacc 102660 tcaagtgatc tacctgcctc ggcctcccaa agtgctggga ttacaggcgt gagccaccac 102720 acctggccat tttctgcctc ttctgattgg gctgtgtatc tgagctgtgt cctgggccac 102780 tgtgccatcc cacagctgac aaaatggctc tcacctccag gaaactctta cctcagcatt 102840 ttttcctctg tcttgagctt ttcttttgtt ctctgggaat agtcctctta ctttcttgga 102900 gcttctttag ttattttttg atcaattttt tttacacagt tttttgtaac ggagcaaaat 102960 attttcagaa cttgtttctg gcatgtgtgt gtctgtctca tctatctgcc tgtgtctatg 103020 ttaaccgttc tttgaatctg atgcccaata ttctatttga ttctgttgat tctgaaaatt 103080 tctttttagt cattggttta gcatttctac ctcttctcct taaaccctag gattttcttt 103140 aaaaagtgtt gggactgatg ttctgttctt cagattggcg aacatggctc aaagaccact 103200 agtgtttaag cagcagcagc ttttctatat tctgcttgct gtgggcactt gtgatgtccc 103260 actgtggctg ccatcagcag agatgtggat cctggtggtg gctgtgtctg cctcctggcc 103320 agccagcttc ctttataggg aagaaatgct tccgcttctt actggggtgt gagaggcggg 103380 cccgtcagtc actgctgctc cctagcactc atagcaatgt ccattttcta tcctttgtgc 103440 cctgatgata atctttctgg tatcataact gtcttctcct ccccttccct gtctcctctt 103500 cctgcttttt ttcttcttcc tccttctcct cctcactcca gtatcaaggc ttagcttaaa 103560 tgtgttgcac tataaaggcc tcatatttta aaaatataat tcattcagtc ctcctctatg 103620 cctaagctta tttctttgtt atggcacttt tcatttcata gtatagctct ctaccgagga 103680 gcgcatacgg gataagggca gagtatgagt attttttgag cccaaaaaat aatagtcatt 103740 tttattggta tagcttcaag catagttcat ttacatgatt tatgttttag gaaatttcaa 103800 gtgcagaaaa attgcccagc agttagagat gattgggtta tcaatatgct ccctgttttt 103860 tggtgtattt tttccagctt tattgaggta taacttacaa ataacattgt atatgtttaa 103920 gatgtacaac ttgatttttt caaaattaat tatttttttg aagtaaattt tattgtgtat 103980 acttaaggtg tacaacatga cgtgatgaga tatatataga taataaaatg attactacag 104040 tgaggcaaac taacgtatcc atcatctcac gttgttatcc aacactaaaa tctacgcatg 104100 gagcaaaaat gcggaataca gtacaatatg attaactata gttctcatgt tgtagattaa 104160 atctctagac ttgttcatcc tatatctgct actttatagc ctttgatcta catcttc.tca 104220 ttttcttccc ttcccccaac cctccccata accactgtgt tatgttctat ttccgtatat 104280 ttgacttaat aaaagattcc atatataaat gagatgatgc aatttttttc tgagtctttg 104340 tttatttcac ttagcacaat aacctccacc aggctcatcc atgttgtggc aaatggcaag 104400 atctcattcg tttttaaggc tgaataatat tccatttgtc catttgtcca tgaattgtca 104460 cttaggttgt ttccatatct tggctgctgt gaatagtgct gcaatgaaca cgggggtgca 104520 gatatcttta cgaggtggtg atttcattcc tttggatata tattcagaag agggttttct 104580 ggatcacaga gtaattctat tcttaatttt ttgagaaaat ttcatactgt tttccgtaat 104640 ggctgcagca atctacactg ccaccaacag ggtacagggt tcccttttct ccacacgctc 104700 accaacactt gctatttctt gtctttttgg taatagaagg gcagggcatc attaatattg 104760 tttcccttac atgtgctgaa caaacacagc agagcagtta ccagcgctaa ctctggaact 104820 ggcttgctca tgctcatctc ttgtccctgc cagttcctaa ctgcggaaac ttgggcaagt 104880 tgcttaacct ctctgtgcct tcctttgtta cttggagctg ataaaaataa tgcttagaac 104940 agttactgcc atataaaaag tgctattaag tgtttgctat tatgtgttga attcagctta 105000 atagaatcat catcaggatc accagcaatt tattgagtga cagtgtgtgt tttattcatt 105060 gatcgtcatt acacttagaa tagggtcggg gatatagcag gcattcaatc aatatttttg 105120 agggaatgaa ttaatgaatg ggctagatgt tatatgtaca aagaaatatg tctaatttct 105180 ttccttcagg tctcacagat ggttgagaat acaagatgga gataagacga aaattgaatg 105240 gtgcttaata tgaagccctt caaggctgcc tctttcagaa tcatttagga ggcttattaa 105300 aattgcagat ttgctagcca aactctcctc gatctcagtt ccatagatct ggacgtgggt 105360 ccaaaactct acagtgccaa gaaagctccc caggggcttc cggtgaattc ccaagaacag 105420 ccaggactgg gatcgcaggc caggcagtaa atgatacagg agatgagagg agggagagct 105480 ctgggggctg acactgttgg gaaggcctgg tgagagggga ggtacctgca agggcatgta 105540 gggcttgggc atggggtgga cttgatcgtg ggggcttaga ctgcagtaga gacattctgg 105600 tcacatttgc ccgcccatga gttccttccg tctcctgatg tgccccagca atttcatgtg 105660 caaagtacac aagagccatt tgttggaaag tacctaaatc actctattta ctgagtgctt 105720 tacttgcact tataaatcta aaacaaattt tcaagcaaat aaacaagtgt gtggatatgg 105780 agcagaaata ccactcatgg catcctgaac ttggccctct gactcagtaa ttagtatcct 105840 aaaactgagt tcaagcatca cattttattt tcaggtttta catacttcat agaacttggt 105900 ctctgatgtt acagagatca cagtaacagt ttaccttatc tccagaattt ctaacttctc 105960 aatggtggca tctctcatta gaacaggtag tcaccaaata gttccacaca aagactctcc 106020 caatgtgtca tttattaaaa caaacaaaca gatttgatat acaattaata taccacaaaa 106080 tcacccatct aaaatgtaca gtctggtagt ttttaacata ttcagaattc tgcagctttc 106140 accacgaatt aatcgtagag tattttcatc accccagagc cacccctccc tacctttagt 106200 cacttcccat ttccccggcc cctgataatc actcatctgc tttctgtctc tgtggagtac 106260 tattctggaa atttcatata aatggaatga tacaacatgt ggccttttgt gactgacttc 106320 ttttacttag catactgttt tcgaggttca tctatattgc agcatgtgtc agcacctcat 106380 ttctttttgt gctgaacact attctactat ataaatatac ttcattttct ttatccactc 106440 attagcttgt acacaactgg atttccactt tttggctatg tgcaaagttt ctgtatgcat 106500 gcatgttctc atttctcttg ggtacccact taggactgga attcctggga catgtgctaa 106560 ctgtatttat ctaacttttt gagaaatctt caaaatggtt cccaaagcag ctgcactatt 106620 ttatatgcct accagcaatg catgagggtt caatttctcc acatcctcac cgacatttgt 106680 tattgtcttt gattatagcc atcccagtgt gtgtgaagta gtatcttgtt atagttttga 106740 tttgcatttc cctaatgata aatgacataa agcatctttt caggcatatt ggccatttgc 106800 atatcttcct tgagaaaatg tatgttcaat ttttttccat ttaaaaattt gatgatttta 106860 aattttattg ttgagttgta aggattcgtt atatattctg gatagtagac ccttatcaaa 106920 tatatgattt gtaaatattt tctcccattt tgtgaatctt ttcactttct tgatagtgtc 106980 cttggatgca caaaagtttt tagttgtgtt gaaaaacagt ttgtatatct tttcctttgg 107040 ttatttgtgc tttatgtgca tatctaagaa actgtttcta atcctgggtc aggagactta 107100 caactctgtt ttcctctaag agttttatag tttcagctct tatatttagg tctgattcat 107160 ttagaattaa tgacttgtat atggtgtgag gtagaggttc aaatctattc ttctgcatgt 107220 ggctatccag ttgtccgagc actaaatgtt tagtccattt tcatccttcc attttgtctt 107280 gtccaggctg ttgaagtagc ctcctcacag ctctccctgc tcttagctct tctccaccag 107340 agtccatctc ccaccatact accagttatt catcttatac acagatatga ttatgtcctt 107400 tccttaataa aaaaacttta ttctttcctt agtttctata aaatagttca aatattcaac 107460 ataaaattcc aggtcctatc tagcatgacc tcaacccacc tcctaatttt actgtgttcc 107520 ttctccccac ttgctacccc atcccagcca tccttgactt cttagctggt ctctttctgg 107580 cctgagtgag acatttggcc tggatatata tgcatggtat ggctttggtt gcttactgtg 107640 gcattggtgc ttatgggata gccattgact ttaattaatg gggtgttatc agcacattta 107700 tcctattgaa atgactagag gaacccataa gaaaccacat tttcaggact agtaatctat 107760 tttagcatca tttggaagac aacaaacttt cattttatgg gtagactaac attcacaaat 107820 tatgacatat atcatataat cataatcatt aagttatatt ttaaataatc aaccatccac 107880 catcccaaac tctaagactt tgttggtaag ttttgtatgt cacaggttca tatttttcac 107940 attctgacac ctacattcaa ggcctcaaac tttaccagag actttttatc attgaggtaa 108000 aactagaagt cattaattta aaaaattata ttatttttaa ttatggtaaa aacataaaat 108060 atacatttta ccatcttgat tttttaaagc agtttactag tgttaagtat attcaacaga 108120 tctccagagc ttttcatctt gtaaaactga aactgtaacc catttaccaa caactccttc 108180 cccatttcct tctcctccca aggcctggca accaccatta ttctttctgt ttctgtgaat 108240 tttactactt tagatacctc atataaatgg agtcatatgg tatttagcac aaggtcctca 108300 aggttcatcc atgttgcagc acatgacagg gttttcttcc ttttcagggc tgaataatac 108360 tccattttgt ctctatatca catttttttg tttatccatt gctctgttgg tagacatttg 108420 tgttgcttcc acttcttggc tgttgtaaat aatgctgctg tgaacacagg tgtgcaaatc 108480 tctttgagga ctctgctttc agttctttgg gatatataca cagaagtgta tataggatta 108540 ggattgcagg atcagatgat aattattgtt ttaatttttc aacttatctt ctcgaatgag 108600 aacactaaat taaatttgtg gggtttgtgt cattttagac atagctcacg aagaagatgg 108660 taactttaaa ttgtccctgc aacaatgatg atgggcttca gtgattgtct taaatgagtc 108720 atcattattt ttgtgtttta taaccaaccc tatgcatctg aacacaaaag tcaaaccttt 108780 ttaatacctc aggtgtattt tacaccaaaa tacagggaaa aggcatcaat caaagctgct 108840 taacagctga tatgatagtg attacatgtg atatggtagt tgagactgaa atgctatttg 108900 taatacaaag attatcttaa ctgagtctga tttgaggtga aaaaaggtac taattagggt 108960 gacaatatga atttgattta actttaaagt attatgagag aaaacatact atgtcacaac 109020 tcttttgtat aacgaactgg gatttaggta tggagtgggt agaatagtgg tcaagagcag 109080 ggattctgga gtcaagtgtt tggcaccaat cctggctctg gccattgcca gctgcatgag 109140 tgccactggg caagcttctc acagcaccag atttttcatc tgtaaaatac ggttaataat 109200 ggattgtggt ggcattacat gaattaatat acgcaacatg ccggaatgac atctggcagg 109260 gaggaaaagc catataagat tttgtaattg attttctagc acaagctatc-cattcttagg 109320 ttgttgtatc ttccttgtgt catcccacat atcaatattg tcatctgcaa aaaatataca 109380 cgtatgtctc ttttaactaa tgattttaaa atgtggacta atcaatgatc taaaatcccc 109440 cttcctttta gtcaattagt agttcataaa ttgaagatgt gatggataga acatttcgta 109500 cccagaaagc tcttagattg taaatgctga gggaacactg ggagattcag tgagtgcagc 109560 acaaatacaa tctaggtgac tggaatagtt tctaatgaat gaatcactga ataagcagat 109620 gggttgtgga aggcaaacca tgagtaaatt ttttctttta ctacagaaat atttctgaaa 109680 ggtaaatacc caggtttatg atgatagacc ttctttaaac tcatgctatt tttctttctg 109740 ggttttgttg tcaacacaat ctcatctgct ctttgcaacc attacctctc agaatgggta 109800 tgaagatcag gtgcaaccat gtatgtgata atgcttctaa aagctatttt ttttccagtt 109860 ttattgaaat atattttgca taaaaatata aaatccaccc atttaaagta caaagctcag 109920 tggttttcag tacattcaca gagttgtgcg atcattgcca caattaattc ctttctgtct 109980 ccatggaatc taattttgaa cattttatat aaatagaaca atacaatatg cagtcttttg 110040 tgtctggctt atttcattta gtatagtgtt ttcaagggtc atttatattg cagcatgtat 110100 cagtacttca ttctttttat tgccaaataa gacttcattg tatggatatg tactacattt 110160 tattttctca ttcatgagtt gatgaacatt tgggtttcca cttttggcta ttacaaataa 110220 aattgctatg aacgtttgtg tgtgagtttt tgtgtggaca cacatcttta attctcttga 110280 gtatatgcct aatagagaaa tttctgagtc atatggtaac cctatattta gcacttccag 110340 gaactgccaa atggctgtac gattttacat tccaaccagc aatgtgtgag ggctccaatt 110400 tccctacact gtcatgtaca cttgctatta tctgttttgt cataatgggt atgaagtggt 110460 aaaactactg aaaaaagtga catattcatg gagtaaaagt aaaattgaaa catgcagaaa 110520 gtattcttca ggcccagaca gccccccaga attacagttt tttttctaga gtccttgaaa 110580 gttgtctatg cacatatgac gtatccctag atatgtattt gcaagaacac atgtaggagt 110640 atactatttt gtatcattct ttatcactta ataatatacc taagagcaat tgctatagaa 110700 caggttgacc tgcctcattc tttttactct tagcagagta ttttttgtga cgatgtacct 110760 tcgttcgttt taacagcttt tctaatgaag ggcattttgg ttggttttgc tcttataagt 110820 aatgccatgc gcatccacaa gacatacaat tgttcttgag tacatttgtg aagatattca 110880 caggaaaaaa caagttatac tgctatatac caccatcaac aggggatgag agtgcctatt 110940 agcccacagt ctttccagct taacactgtg tgttatcagc ctttcccatc ttggccaatg 111000 tggtaggtaa aaaatagttc agtaaaagtt gatttgacct ataaggggca aatcaagttt 111060 atgttataaa gcttgaagtt caacattgaa gttgaactcc agataatatg gttaaagctt 111120 tcaaatttct gagaaatatg caggcataga gattgtgtct aaatttgcct taatacatag 111180 tatatccatt tgctaattga agaaggtgat tggttcattt atttattcaa ctggcattta 111240 ttcagcattt atcgtatgct aggtgaactt taagtgctag agagaaggat gaataagaaa 111300 cacacattgc ttacaagtaa tatatagtct aataaggggg acatatatat ataaatcaat 111360 aagcataatg gacatgaaat agaggcaagt attatacaat aagctgtgta tattaggcca 111420 taggctaagc tactgtgaaa aaaaaggacc ctcaaatact tgggaaattg cttttgggaa 111480 aagacaaaaa aaaaaaaaag cttgtggaaa agggtgaaaa gtggacatga gagtaaggag 111540 agtaagccat ttttaaaata aaattttatt ttaacttcga ttgtcatatg gtaagtgtac 111600 atattcatgg gataaaaatg aaaggttttg acacatgtgt gcattgtgta atgatgaaat 111660 cagagtaatt agcaaatcca tcatctcaaa catttatcat ttctttgtgg taaagcattc 111720 aaaatcctct cttctagtta gtttgaaata tacgatgcat tattgttagc cacagtcacc 111780 ctgctgtgca atggaacacc agaactgatt cctctcacct aactgcaact ttgtaccagt 111840 tgatcaacct ctccccatag ccatttcctc ttaagcaaga aacatggaaa ttgtacacat 111900 tacttctgaa aatacactgg caagccactt ctagttgcag ggtagacttg gaaaatgaag 111960 tctaactggg tgaccctgtg cctccacatt attctggagg aaggggatca cggagctagg 112020 gtccccagtc tgggaggctg gtagagaggc agctcgctgt gctactctga tatttttagc 112080 tccgtggggg aaaaagaact gctagacaga gctctgtcaa cacagatctg acaatctcat 112140 tcagaaacaa gttacagaga cctcaagtga ttaataaaca agattattct atagaacatc 112200 ttatggtgac ataatcaata tgatttcaac actcagctaa taaatgctat gaaatacaat 112260 ttatgtaagg tgttaattga catgtcaggt tttaaataca aaaggaatga gggagggaga 112320 catgaatata gatgggtgtt atcagagaat gacacttaga tacatttcat attgtgtcat 112380 gtaaactaat acctactttt tgggatattt cttcatggtt tagtttttaa tgtagattca 112440 agagttttat atattcaaac tcttcatgaa aaaaattcag ctctggttat gtacatccac 112500 atttacagga attgaatttt tctttttgta cacttgtgag tatatttttt cctatggcaa 112560 acgttttcca gaaagcaaaa gttatttgtg ttactgtccc tgataatcga tgctgtgtaa 112620 taaatcgttc tcagcaatgg cagtttggac actgatggac agttggtgac tggtaatttg 112680 tacagtatgt ttagtgggac ggcgggcaag ggggaaaagt ggcatttggt tcctcagtcc 112740 cagatttggg tcaatgttct cataatactg gtgagcaagg agcctaattc tttaggaatt 112800 ttaaaaggtt tcttttagtg aagaacattt cccaggtgtc taatgaaaaa ttataaatga 112860 ccaggtataa ttttgccaag ctttccaaca tagttttcct ttagggtgag aaacaatcct 112920 ttgattttct tgaagtagaa gtctcttctc gaatgctaac agtaaaacaa gaccttttca 112980 gacagatttt gtattttgcc atcaaatgtg caatttttga agatacggga cttgagaatc 113040 aggtaaactt cgttctgaaa accaggctta attttatgac tgtgattaga aagttgaaaa 113100 aaaaatcacg ttctcacaaa tgaaaacaaa agtcagagtt caaactgttt atcctgtttc 113160 tgtaagcttt tgtttcagaa ggccaaggat tatttgagga gaacctggaa aattaactca 113220 aatggaggaa aaaaatggcg tttatgcaaa atgcagcaaa agcagacagc cttggagaac 113280 tgaagcttaa atagcgtaag agtctgagta ctagtcttca gatacttaaa acagcatttg 113340 atagacaagt tagatattta aggaaacatt taatcagctt ggcatcagca ttagtggctt 113400 caatttttgg ctgagcctga ctttgccata tgatcctaga caaatttgcc taaaatgggt 113460 gatattaact tctttaggga aaggctctgt actgggattt ttttgaggcc tatgatttat 113520 ggggtcaagc tataaaacag gaggaaacac atgctcattt gtataatttt tacatatatt 113580 tggtccataa ttgtacagct aggcaattca tttattatct gtttgagagt aaaagtttga 113640 ttattttaat gttactactt ctggtttttt ctcttctcac atgttcccag tgactccttt 113700 ctgataaccc aacttatcat catagaacgc atgtaatgct gagaataaga cacagggtct 113760 tggaaaatga atgacagcaa tggtgctccg atggcagttt tgtcagactt tgaatggttt 113820 atgaaatcac tgttgataaa agtgaacacc ttctactgaa gggagaaatt taggggggaa 113880 aaatcccaaa tagaaggagt taatatccaa acctggagac ttacctggta aggttcactt 113940 aactggtaaa atgtgatcca atttaaacaa agtattttta gttttctcag aacaaacatc 114000 ctacataaac acaaaaaatg atatgagaca tagatataac ttggttcaca atattttcca 114060 aaactataat gtaccagcca gttggtacag cacaccagga gagaagatca ttattaatgt 114120 gctaatagca gcattttatt ttgaaaccca ctctgcatgg ttacagggct caaaacaaca 114180 tattctaaca ggaagataca ttaccgaaat attttaatga gaatatttaa tatgcattga 114240 gaggtccgca ttttcttgca gagaccttgt aggtagctct ttgagatttc tgtctctatg 114300 catttaagtg aaggagttgg ttgggtattt tagttggcaa attttgcaga catgtagctt 114360 tggtagtgga gaggtaatag taccatgccc tgcgtgctgg cgaggaagcc ccacagcaac 114420 agtggctttt agcagctacc agatttgcta aaagcagcca tgtccaatta gcagtaagtg 114480 ccatgcacct gcagttacta ggaattgaac ctcttttgag gctgaatctt aatgtagcct 114540 tttaaaaaaa tagcaaaaat cttactcata ctctgagata ataaagaaaa attagcaatg 114600 gcaaaatgga cgcactctga aatgtattct taataatgat ttagaatatg gggtaaatgt 114660 agaggcaatg acacatttaa actgcattat ttttaaatac tgttttatca gtttacttcc 114720 taattttgaa ttcaattttc atatctatac tgcaactgct tttttttttt ttttttagaa 114780 atctataata ttgctgcagg ctccctatgt atgtttccat aattttctgc aaagtgtttt 114840 caccagaata aaaaaaaatt acagttcaaa attgcaaaac tgtagaaaaa atatgctctt 114900 tgacttcttt tctatgtgtc aaattcacca caatggaaag gactacacta tagaattaaa 114960 acgttatttt caacagatag tacttatttt aacatgtgtt cagcatttaa aaatatttaa 115020 tattcttttc taaaaatgct tacattcaga aactatttat gagggttctg agcagtatga 115080 tgtttctttc tctgatttac tgctttttct cttttagagg atattgtagg agaaaaacat 115140 ttgttaagca atttccagaa ctactaggtt ctaataagac caataggcta ataattcatt 115200 tcattgcaac tggagtgtgt acttttcctt tattctcagt cataattttt taaaagagcc 115260 aagaacacca aatcatcaaa tctaatgttg aacatgtagt aacttttatt tgctgatcat 115320 actctgagat tcaccataac gaataaatca taagtattaa aatttcagct ttttaacata 115380 cccactacac tgctagcctg gtaacacagt caccaactac acagcttctt caactgttga 115440 cgtgctttaa agctaggatt atggtatcct gccaaaaaga tcctacaaac tgtcactatt 115500 ttggtttgtc ctgccagcca ttgagaagta gacaatttct atatacacaa accagtttgg 115560 aaaatttatg caaataggaa gttattctca aaatgggtta tttgattgca tttttttttt 115620 tttttttgag acaaggtctg actctcgccc aggctggagt gcagtggcgt gatcttggct 115680 cactgcaacc tccacctccg tggttcaagc gattctcctg cctcaatctc ctgagtagct 115740 gggaatacag gtgcatgcca ccactcctgg ctaatttttg tatttttagt agaggcaagg 115800 tttcaccgtg ttgactaggg tggtctcaaa ctcctggcct caagtgatcc acccacgttg 115860 gcctcccaaa gtgctgggat tacaggcata agccaccgtg cccagcctga ttgtgtttta 115920 atgtattggc tcccaaccag tagcaacagt ttgggtgcac agatattgcc gtagtgtttc 115980 tattcaatgt gtcaaattat ttaggtaaaa gtttggctta actttgttga catggaattt 116040 ctatatcctc cagtttctgg tttaactggt ttatgtggaa atgaatggaa acacatggtt 116100 aagttcccca gcctccagct tctctcttcc tcttacctat atttgtactt gcccactttt 116160 cctcttgtgg gcctgttcct agaatccatt atgttctgtg ggtgacatca gcctgctact 116220 gcgggaagaa atagaatttt atggtgccca agataaaatc gactatgatg atgcagaatg 116280 aagacctgga gaggagtcag agattgcagg ggataaaaag agagaaatca gagatgctcc 116340 cacaaacaga gaaaaccttc agaagcagca ggaaggacag agtaaatgga gaacaaaaac 116400 tcaagtcaaa atagtcaatg gaaataaaca gttttgaatt ccagttataa tttggaaaag 116460 aagcaaacgt ttattttaaa ttaagaaaaa agtcgcttat aatttcctga agtttagaaa 116520 agtgaaaaat agttaaacct cctgttgtta gctaagaagt gtctattatt ataagtttgt 116580 cgctggagag aacataaaaa tctctatcta ttgctatttt tttttttttt tgcataaggg 116640 atagataaaa gagtgtttat tttatttagc ctttggcctg taaagtaaga tttatgaaac 116700 aaaggacaat cggaaatgat aagaattttt tcctaatttc taattattta gctaaacctt 116760 gattgatttc tctcaggcta gtgcccatat tacagcagca gtcagccttc atttatctta 116820 cctgggacca ctgctaaatc cgatgtcact tttcagtttt tatattatgt gaccatttag 116880 ccacatttca cctacttgat cattccctcc atcttgagac ctccctcgac ccggcttcca 116940 acatactttg ccctcctggt ttttctttct ctgttggctc actttctcag tctcctctgc 117000 tggttgctcc atttttccac aatctctcaa aactggtgct cctcagatta agtccttgga 117060 cttctctcct tagattgaca tctggagtca tggcttcaaa caccacctat cactcccttt 117120 ctccttgaac tctagacgtg tccatccaac tgctacgtga catctccact gggatttcta 117180 agaactttgc caaacttaac atgtctaaaa ccaaattctg ggctgggggt ggtggctcac 117240 gcctgtaatc ccagcacttt aggaggctga ggtgggcaga ctacttgagc ccaggagttc 117300 gagaccagtc tggccaatag agaaaagctg tctctacaaa aaatacaaca cttagctgga 117360 cgtgatggtg catgcctgta gtcccagtta ctggggaggc tgaggcggga ggattgcttg 117420 agcctgagaa gttgattctg cagtaagcta tgattacacc actacattcc agcctgggtg 117480 acagagtgag accctgcctc aaaaaaaaaa aacaaaaacc aaaaccaaaa ccaaaaaaaa 117540 aaagcaaaga gcaaaacaca aaaaacatat tccaaatctc ccctttaaac ctcctcttct 117600 tacaatgagt cctatcttga tttatggcag atccttcctt ccagttctca agccaaacac 117660 catggggtca ttcttgactc ctctcttcca tgggctcatc taaacatcag agtatcctgt 117720 tggctatctg gaatttaacc acttcctatc actgccattg ctacccacct gctcttagtt 117780 atcaccagag ttccctggag tgttggagcc gccgcagagc tggtttccct gctcctgcct 117840 ttcctgccca tgctctgtcc tgccagtcag ctctgtcctt taaaaatgga aatcaggtta 117900 cctcatgact cttttctttc tttttctttt cttttttttt ttacagaatc ttgctctgtc 117960 atccagcctg gagagcagtg gcatgatctc tactcactgc aacttccgcc tcccagattc 118020 aagtgattct cctgtctcag cctcttaagt agctgggatt ataggtgcca gccaccatgc 118080 ccagctactt tttgtatttt tagtagcgaa ggggtttcgc catgttggcc aggctggtct 118140 tgaactcctg acctcaggtg atctgcccgc ttcagcctcc cgaagtgctg ggattacagg 118200 tgtgagccac cgcgcccagc catgactctt ttcaaaaact ccccatcttc tccacttcca 118260 ttcagtaaca gtcagatctg catggccccc tcctgacctt ctgatgtcat gttctttgct 118320 accccttgag tgcattgaac atgcctctga cccagaactt ttctgtttgc tatttcctca 118380 agtgcatatc ttgttccttc attttcttca ggtgattatt aaaatttcac ttccatgagt 118440 ccttccctgg cccccatatt taacactgca tcttcctcca tcttctttcc ctcctcccca 118500 tgtacatttt actcccttac taaatttttt ttcctatctc actttctttt ctttttgaga 118560 cagagtatta ctctgtcact taggctggag cgcagtggcg caatcttggc tcactgcaac 118620 ctccacctcc tgggttcaag caattctcct gcctcggcct cccgagtagc tgggattaca 118680 ggcgcgtgcc accatgcctg gctaattttt gtatttttag tagagacagg gtttcatcac 118740 gttggtcggg ctggtctcca actcctgacc ttggcttccc aaagtgctgg tattactttc 118800 taacctcctg tgtatgctac ctatttattt gtctgtctct ctacactaga atataagctg 118860 ctatgagggt agacttcttt gttttgtcca atgctctatt tccaatattt ataaccgtac 118920 tggccggtag gtactgtcaa ttatagtttt tgaataaatt gagagtaaca aattctaact 118980 ggtgaaaata aattaaatgg gccgggcgtg atggctcacg cctgtaatcc cagcactttg 119040 ggaggccgag gcgggcagat cacttgaggt caggagttca agaccagcct gggcaacatg 119100 gtgaaacccc gtctctatta aaaatacaaa aattagctgg gtgtggtggt gcatgcctgt 119160 aatcccagct actcgggagg ctgaggcaca agaatcgctt gaaccctgga ggcgaaggtt 119220 gcagtgagcc ggcattgagc cactgcactc cagcctgggc gacagagcga gactctgtct 119280 caaaataaat aaataaaata aaaattaaaa agaaaataaa tgatctcaca ggatatcatt 119340 tcttgattgg aggaggagag aaaaatgtgt atgtgtgagg gtacagagag agttcctttg 119400 cattagtgag ggttcacatc agaactgtct aacctcccta ctcagtgggt cctgttacct 119460 ctctcctgaa tccaataata ctctggtgca caccttttac tggtactgag gctattttaa 119520 cgttccatta aaaaaaaaag aaaacattta aaatacaaaa acaaggctgt actactttaa 119580 ctctgacctt agaagactct cagttttata actgtttaga gaaaatgtgg aaatgtttgc 119640 tggccccaat ctttttatta ctcatctcta taaagaggct tttaaggaca tattaaaaag 119700 tttecatctc tcttgtctct tgcctttctt ctcatcttcc tttcttgtac cccaaatggc 119760 tgaaagcaag accctgagag tctggaagaa atatattctg gtcagagcag actgagctgg 119820 agctggccta gaaccttgga tcctgacctc agaattgctg ctcaaattac tgctctctga 119880 aagagtgagc tcatgcatgt ttattattac tgtgaacaaa tttagctgtt gtttcaccca 119940 aacattgaag ttctcgcttg gacccctttg cttgtttgtg cttttgcaaa tgtgttttct 120000 catgtgctac tagaaccaag atgaaagctt cagttctcag aacaggtgcc acgaccgagg 120060 agacaaaaca agagctatag aggggtgtgg tgtgtgaaac tgagaaagca ggtagttttt 120120 cagaaaaact gagagccagg aatgacttca tccatttcct gtaataaggc aatggtctga 120180 ccttgaggaa cctattagag tgtgcttcta aacagaggcc atttcgaggc tgaatgtacg 120240 ggagtattta cctttgactg ttttctgaag caaggtattt tataaactgt aaagtggctt 120300 atacaatttt aggaatggaa agttttgcaa agtctccaaa atccctggga gttttgaatg 120360 tgcaggccat atgaattctt tgtagtttca tggtcttgct gttcagcatt ctttagagtc 120420 acctgcatgg tagaagttta gtaggaattt gggtagttat gggctggttt ctttgttttc 120480 ccaaatagag gtagcaaaat ggatgctaag aagcaaatta cttttactat tttagatgcc 120540 tctggggaca tcctgtcata atgcaactta aaatatactc gtctcctcag ttccttttta 120600 tgtatttatt taatagtctt caaccaattt gttcttcatt agctgttcta caatacttca 120660 agagaagtca tgataatgat gcatttagga tcagaaaaat atagcatgtg tgagttcctg 120720 gggactttag agaatatcta gcccagtctt tcattttata gaagaggaag gccagggatg 120780 gtggctcatg cctgtaatcc cagcactttg ggaggccaaa gtgggcggat cacctgaggt 120840 caggagttcg agaccagcct ggccaacatg gtgaaaccgc atctctacta aaactacaaa 120900 aattaggcag gcctggtggt gggtgcctgt aatcccagct actcaggagg ctgaggcagg 120960 agaattgctt gaaccaggca gaggttgcag tgagccaata tcatgccact gcactccagc 121020 ctgggcaaca gagtgagact ctgtctcaaa ttaaaaaaaa aaaaaaaaga agaaggagag 121080 gaagctgggg gtgaggacag gcagtgactc acccagggtc tcacactgat ttgctggcag 121140 agttaagcat gaaaaggtgc ctctcctagt ctctttccag tgttcttgtc atcacaatga 121200 gttaaaataa aaattactag caacttggga gtaataacaa taaacttctc aaaatctact 121260 acatggtcta gaaaaccaaa cataaggccg gacgcagtgg ctcacgcctg taatcccagc 121320 actttgggag gccaaggcag gcggatcagg aggtcaagag atcaagacaa tcctggccaa 121380 catggtgaca ctctgtctct actaaaacca cagaaattag ctgggtgtgg tggtgcgtgc 121440 ctgtagtccc agctactcaa gaggctgagg caggagaatt gcttgaacac gggaggcaga 121500 ggttgcagtg agccgagatc atgccactgc actccagcat ggcgactgga ctctgtctcc 121560 aaaaaaaaaa aaaaaagaaa gacacccaaa cataaaacca gaatattcct gtgagttcca 121620 ttacatagga caatatggtc ctgctgatct attaaaatta tgtttataaa ttagaggctc 121680 ttcattggga gatatactta atgctagatg acgagttagt gggtgcagcg caccagcatg 121740 gcacatgtat acatatgtaa ctaacctgca cattgtgcac atgtacccta aaacttaaag 121800 tataataata ataataataa aaaaagaata acaggaaaaa taattagagg ctctttactg 121860 tatttaaggt gttcaaataa ttgaagctag gttagtatct ccttagcctt tccccagcat 121920 gccctgcagc tttgacgctg cagaacacca gttggtggct gatatttaga tgatggaaat 121980 acttggacac attttgcatt tttgataaaa tgacatggca ctgatggtta cattttacaa 122040 atatttttgc ggatgtatat acaggtggca tacccaagaa ggaacagacc ttcctcaggg 122100 gtccttctta ggaaagtaca gctttgcttc tctgatcatc agaattggat ccttcaggta 122160 ttaggttggt gcaaaagtaa ctgcggtttt tgccattcaa agtaatggca cctgaaaggg 122220 ttggtaaaat tcagaatatt ataaaatcag taaaagctta atcaaatttg cctaaatcta 122280 tcctacagat tagtctatgc atggtttatt aaacaaccta actgaaacac acatgcatat 122340 gtgtgagtaa gctgacatca gggatcacct tatcatggaa aatctaggga aggttggctc 122400 aatcttcaat ttgtgaagac caattcccct accacgtcca tatatacata ttcccaggag 122460 acttgtttct tcagggctct ctttggttct cagctcttcc tttctgaatt agtgggtcac 122520 tttaacctcc cttgggtcac agtcgtgttc cacactgggt gggtccagag gtagaactca 122580 ttgcctttct ttgaatccca cacaagacca cccaacaagg cattgtagtt ggtggaaaac 122640 tccattgaag aaagggtcta tattgcaaca aacaagcatc tagaaataga atattaaata 122700 tccctcctct taaaaatcca ttgtccaatt cagacttcag cataatgtaa tcttctgtct 122760 tcttttctct gtgtttccat cttgagagcc atcctattat ctgtgtcctt tctttgttgt 122820 cttaggaaga atattttggc ttatcctttg ttttgttctc tccttcccgg acctattatg 122880 gtaaatgtgg aatttgcaga taggcttatt tgcgctcccc agcctgctcc cttaatgctc 122940 tctggtaaga cacttctttg agtcctccga ggcgagttgc tgccccccag cacctgctct 123000 tccccatcgc aacccatgac agtaaacgca ctgcacatac ttatcccaca gactgctgtg 123060 agaactagat ggaggctatg tgtgaagctg caagctcttg gtaaagtgct acagaaattg 123120 gtagacactg ttactatgat aagatgttca gaaaataatg gatcgattaa agtttcactt 123180 atctattaaa agataaaaaa ctctatgatt gagacctttt tggaatattg gaaaatattt 123240 ttgtgtgctt attagcatac tgatatgttt cagtacacat ttcatggatt tatggctaag 123300 gatgttactt tcaagaaggt aactatacat cgctctctac tagcatgctg ttctgttcct 123360 gagaaatagt ggctgggcga agggtatgca aacatttctt aatatctcat ccaataccat 123420 tttcccacta ctaattattc aacacatgga aatgtaggtc aaagcattaa aaaaaaaaac 123480 tagaacagca ataccttggt ggttggcagg tgtttattta aaatatttac gttttagcct 123540 tattttttaa tacttctttt tcatatatat tagatattta actaaagaaa ataataatca 123600 ttctcatgga atactttaaa atagtagaaa caatcatgaa tagaaatgaa gatttaggcc 123660 tatactaccc taggctctgt cattcttatt cctaaccatt tgcataggaa atttagattc 123720 acccacttct ctttcttaga ataagattca caggtttttg ggcatattcc cattgcatgc 123780 ctgactgggg aggtgttcaa atccctgctt ttctactcct cagccctgtg accctgagca 123840 atttctgtgg cttggtttcc tcatctgtaa aataggatat taatagaaca tatcccttta 123900 acgttgcagt gatcacatta attagttgat gcaaaacagt ccatctagaa cagtaagccc 123960 tcaataaatg ctagctatca ttatcatttc atagccagcc ccaccatgga tgggtcatgt 124020 gacctgaagc aaatttaaat tctctggatt tcagttatct ctcattgaag gaattaggct 124080 ccatcagtgg ttctcaaact caagtgagta tcagaatcac agggagggtg tgttatgaca 124140 ctgaaggccg ggccccaccc tcagaatttc ttattcagta gatccaggag ggagtgcaat 124200 aatttgtatt tctaaaaagc tcccaagtga agttgatggc tgatctggag acacaccttg 124260 aaattcttct gcttactcta aacaaaacgt gcttttttat cttgagtgca catcagaacc 124320 ctctggggag gttaaaagtc ccagtgaaat cacaacctct gcagatggta accagatctt 124380 agtggctttt aaagttctcc aggtgcttcc attgagcagc taaggttgac aacacttcta 124440 acaccgcttt caggtgaaaa agaactatca ttctaactta aatgaaggag aaaatgaatt 124500 catcattctg acaccgttat ctattctttc atgcatgggc ttatcctatg agaaataaac 124560 atttactaag gtgtatgcat gtttttagaa catccattgg gaagcatgtt gtacagcttg 124620 tagtaaaaca gcatggtgca tgctgaaact gttataggct ttgcaagtta ttttttataa 124680 attagttttg ctggtgtgct gtttgattat tggagagtgg tgtgaacatg tttctatgca 124740 tcccaggggg ttgctgtcac ctttaagggg cctctttaaa tcccagctct ttcatttaca 124800 aattctatga ccttggatga gtttcttcac ttttctgtgc cacaagttct cagtaaaatg 124860 gagataatta tagaacttac ttcatagagc tttgagaatc atacaggtaa agttatttta 124920 cgtaattagc actcaaaagt gaccattaat aatctaacgt tttaaaatat attaataggc 124980 cagatgcagt ggctcacacc tgtaatttca gcactttggg aggctgaagt gggtggatca 125040 cttgaggtca ggggttcaaa gccagcctgg ccaacatggc gaaactccgt ccctactaaa 125100 actgtaaaaa ttagctgggc atagtggcgt gtgtctgtag tcccagctat tcaggaggct 125160 gaggcaggaa aatcgcagtg agccgagatc acgccactgc actctagcct gggtgacaga 125220 gtgggactcc atctcaaaaa caaacaaaca aacaaaaatt aatagtttaa atatttttaa 125280 tatatttcaa tatataaaga tgtaattttt attttaaata tattaaaata gataagttta 125340 aaatacataa agtaaatata taaaattaat atgttaaaat gtttactgaa agtttacatt 125400 tttctctgta ttgctaagag caatttattg ccttatatgt tgtgtaatat ctagaaaaca 125460 cacctatgta atttccaaat tataaccacc ttggctcctg gacaagtagg actgtgtctg 125520 attttttttt ttgcatcctt gtcactacct gccactctga gatcacttgt tgtctttcca 125580 tatataatca cctctaggta cacttctttc attatttcta cgcatcctct ccactttgct 125640 gcattcttgt gtgcattctt ttctcccacg ttggaaagaa atatgcatat tgagcgattc 125700 caacccccaa aggcaaaaat tactagccag tgttgaaaat gaaagcaagg aattttttgg 125760 ttattgttgc tgtaactttt tgatgtgcgt acatatgtgc gtcttttttt tccccctagg 125820 ggtggaatgg ggtggtgtta gtcagggatt actgacagga cggtgttgcc tactgtgttg 125880 gagagactaa gactggggaa agattctcca aggctgggaa atgcagacgt ccagtcttgg 125940 aaaacagttt gtcgttggtc gttatgtcag aagaggagtg tattcctgga agaaggccca 126000 caaaatatac ccagagccca ggtgcctttc taagacgaat caacattcac tcacaagttc 126060 agctaatata tgacagaaat acaagaatga acaagactcc tgacttaagc ttacagctga 126120 tgggaaactt gggaactgga cagagaatta aaatacaacg tggttaggta caacttacct 126180 gcgccagagg agtgacggaa ggccttggac ggggagtgat gcctggagga tgacagggcg 126240 acaggtaagt tagggggctg ggaaggggaa gggcggccct gtgggtaaag ggaaggaggt 126300 taaagtcagc agggaaacag gcagttccct gttgctggag caaagcgcgg gggtgtggct 126360 ctcctgtctc tggctttgct gtcaacaggc gagattggca agtagggcga ccaaccggcc 126420 tggtttgctc gggaccgggg tttcctggaa ggtgggactt tcaatgctaa aactgggaca 126480 gtcctgggca aacaaatcag tacggttggc caccctatct ttgtgtgggc atctgctaac 126540 tgagtcatgg gcctgtctgg tttatttatt tatttttttt ctgcctgagt aacagaagaa 126600 tgtgttaaac atatggagta tggaagaaga aggagagggg agataggatt tttatgttat 126660 agattggaag aagacatcct tgatgaagga gatgttttta ctttggagaa ggactgcctc 126720 agccgcgaaa gaccaagggg tggcaccaca gggagaagtc tgcactgcgc ttgtcaggac 126780 ggccagtggg gggctgctga gcgcagggag tcagtcctcg caatgaagat atagtctcgg 126840 ggagataatg ctggaaatcg atggaagaag tggaacagga cacaaattta gtggaatttc 126900 tttacaatgt gtgcttcttg tcaccagttc gttaaccatg ataaaaacgg ttatgttgtt 126960 acattcatca agatccaaat tttctaccat tttaagatgt ctatcagtca caatgatgat 127020 cactgaagac cgaacgagat catttcctct aaaaattatg ttacatttat ccataatggc 127080 atcagtgtct gttacacgtt tgctttaatt ttgaggtcaa atacgacatt tgtaaatcta 127140 gcacttcatg tgattctgta agcactaaac agctaaacat atttacttct tttttttgaa 127200 tcaattagaa cagtgctgta caatagaaaa atagaacagt gctgtacaat ggaaacagtg 127260 ctgtacaaca gaaatctgtc agccacacgt gtcgtttaaa cttttctagt aatcacatta 127320 aaattgtaaa aagaagccaa taaaagtaat tttaataata tattttgttt gacataatat 127380 agctaaatat caattttgac atgttctaaa tataacaatt attaatgaaa aatattacat 127440 tcttttggct ttgtactaaa catttgaaat gtggtgtacg tttttcactt atagcacacg 127500 caattcagat gctacatttt tattaggaat atttaatctg tagatagata tcgtaaaatt 127560 tacatttgaa aaaaatagat tcagacacct aagttgttac aagcatactt aaaagttttc 127620 aatgactgaa ttgagtatca gttttaaaat ttaaatcaat gaaattaatt aaaatgaaat 127680 gaaaatttag aaactattcc tcagtcacgc tggctacatt tcaagtgctt catagccaca 127740 tgaaagctgt attggacagc aaagaagtag aatatatttg gaataaaaat tttaaagtgg 127800 acacattgtg ttactctcgt tagccatgct attgctattt tttttcctat agctaattaa 127860 aaccttaaga tccagtaggt tctccacctt tttttaaagc attagttcca tgtcgaccct 127920 gtagatggca gcactttctt cctaaaacta catggaggag ttgcctgggc ttgtcactca 127980 gattctggca cttttcatag aaagagtctg aattatctgg aaaattcttt ggtaacatag 128040 gtcagaatct tttcagctct attgttattc tgcacagatg gctgttgctt atgaaaacaa 128100 tctctcagcc tctagtccag gatattactc attcctcagt tcaagaaact ctagggtgag 128160 aggagaaagg ggttcaatta cagaactgtt atcaaaatgc gttggtttat gcacatccgt 128220 gttttggaca tggtgattcc aagagactct taataaaact tttcaaagta gatgagagac 128280 agtttttccc tcacatgctg tggcaatatt aatctatgtt ttcatgttcc actggacttt 128340 gtaattgaat tttaaggaat gcatacaggg cttcatattt atatataaaa tatccatatc 128400 cagtgttgaa agaaattaac aataaaatat gtacctgtat aaaatttgtg atttttgaag 128460 cacccctctc ttcctcttcg cattgcattt gtggtaggag aacatgagac aaggaacgag 128520 gtactaagga caaagaagga gcgattcaga ggtggatttc ctgaagacca acaacatttt 128580 tgcatagctc gtgaggactc tctgatacta aactccaata aatgatggtt tggtgttttg 128640 attgcttttg attgacattt aaaaattaca ggagactgag ataggaggat tgcttgagcc 128700 tgggaagcgg aggttgcagt gagccaagat tgcaccactg cactccaacc tgggtgatcg 128760 agtgagaccc catttcaaaa caaacaaaaa aataaaaaat aaaaaaaaac aaataaaaat 128820 cacacgcctc tttttttcac tcaatctgtt ttccaaataa aatatcaaga ttctatttga 128880 attttaataa tgattttgct gagttttata gcttgaatac tatggcaagt ataatgtcta 128940 aaatgctgtg attttgactt aagaaaaaat ttacagtttt cttaatatac tctttagttc 129000 ttttaaactc taatacatga aatggattgc taatgagggt aggaaggggg aaagactggg 129060 gagaaaaata gctaactttt ctagcgatag atacatgtca gaagctgtag taggttcttt 129120 tgcatgtgtt tacttacatt accttctgta atctgcattg caattttttc atagacaagg 129180 aaacctagac ttagagaagt acaatacctg tcatctcgta aatgacagat atagttgagc 129240 aaatctactg aaatggttta gcttcttatc tttccagaca acagataagg acttattcta 129300 tatgaagaca tctgagaaat gaaaatatgg tttcaggttc atagaatctt ttacaccacg 129360 tatttctggt tctctttctt ctattcagat cattctttca tctgtttctt ttgtcttctt 129420 ttccaaaatg tagcaattgc cccaaattca gtacttgtct ctcttcttaa caatcacatc 129480 caatcccatg gcctcaacca tcttcccttg catataattt ccacatttac aactttattc 129540 tcttgattac tcaggataag ataattgctg aacaaataac agcatatatc tcagcttaag 129600 agagtaaaag tttatttttc tctcatgcaa attctattgc agatattccc cacctccttc 129660 cctaggttgt cctcaaaatg cagtgactca gggaactcct ctttctgtct taacctattt 129720 taggtcttag acccctttgg cagtctggtg aagccaataa atctcctctc aggacagttt 129780 ctaaatgtga taaaagaaaa aaaagatata gtgacagatc taataattaa aattcaaagt 129840 taatttcaaa ttagtgatag gtaaatatat ttcaagatgt ctgcactgaa tacagcgtga 129900 taggtaatat atatgattta tcttggtgac aaagacattg gtattgctaa cactattata 129960 gctgccatct gtattcacaa agggagatgt caaattccag ttaaaagaaa gattttctta 130020 acccaagttc ataggacttc ctgaatggta cagatgctaa ggtctagtgg ctccacaact 130080 accaaagcct tcttagtgtt ctctgctgga tcttatgcat ccaattgact gacaagcaaa 130140 gaacctgcag aataatagga gacatttgtt gggtggggag agcaaaccta gaaggggcat 130200 atacccctta caattatagc tctatgcgct agaattagtt atatcattcc tcctagatat 130260 gaggggacta ggcactgtac ttcagctgtg tgtccaggaa aaaggtaagg tatggggaat 130320 gtggagcaat aattatttga agcaagaatc accagtggat gctaaaattc gtgagggaaa 130380 acaggaccag aaacaggata tttttaaagt ctccaagtac ctccacatag gatacttatt 130440 aattgcaaag ggaaaatagt aactttacag agaagaagaa atctggcaga cactaccata 130500 tccaagtgat caaaggtgac atcagtagtg ggacacatta acatcatgtg cttctgatat 130560 gatacacaaa ggagaaacaa cctcattctt gtgatgttcc tgccaaaaat ccataacctg 130620 aaagaagcat cacaaaatcc ccaattgagg ggcattccac aaaatagctg gctagaactc 130680 ttcaaaattg tcaaggtcat gaacagttcc agaccaaagg aggctaaaag agacaggatg 130740 cctcaatgca agtataatcc tgaattgggt cttgggtcag aaaatgggca ttagtaagac 130800 aattggcaaa atttgaataa ggtcaataga tttgaaaata atactgtacc tatgtgaatt 130860 tcctgacttt gatcaaagca ctggggttaa gttaaaacat aagatgttgt ttttggaaat 130920 acatgctgct atatgcaact tactctcaaa catctcagaa aaaaaaatag ataagttcat 130980 agatagatag atgacaggat aataaagcaa gtgtgataaa atgttagcat ttggggaatg 131040 taggtaaaag tatatgggaa tattttgtac cattttctca acctttcctt taactcgtaa 131100 attacttaaa agaaaagcaa taaccctggc tcttataaaa ctaaggaaaa tggtccatcc 131160 tttcctgtgc cttgtgtttt cctgctggtc aaagaacctt tggtaatgac aatctgagtt 131220 ggtcactgat attctagtaa attcagaata taacttcctg ggctatgtag aaaaatatag 131280 ctcttcatat tttaattcat tttcctgaca gcacgtttct gtgcttttat gtatggatac 131340 tgcctagtaa tgtgcacacc agtcctgcag atcctggaac attttattat cttgatattg 131400 acttacgtat ttaggtagat ctgaatagca gaattgtcag tcgtttcacc aagacattat 131460 cgggaaatct ggacttggaa gtctacttct gccaggcatc tctccacgct ggcttctggg 131520 ccacagaaga aaagatctaa tcaaactcat ttattaacaa aggcttataa tattgaatag 131580 cagcttccag gaatctaaga aaatgttcac ttttatttct gactaaggag gaactattat 131640 agtcttttta taccagttag aagtgatgac ttggtattaa acttattcta attctcaata 131700 gccctgatct cgactctgac cacatgcgcg cacaccctcc cccaaccctg taagtgtgat 131760 tatggcaagg ggaaagttaa agtggagtga atggtgagtc gtggaatgac agagagcatt 131820 tagcatttgt aattgacaga gttggtgatt gactgaatgg acactgaata tctaggctaa 131880 aagtaattga atgtggatgg tgtagtgaga tggagaacac tagcaggaag atagactggg 131940 ctacgcaggg gctagatcat gtgttcagtc tgggatatac taagtttgag gtttctgtaa 132000 aatgtccatg tgaagatgtc caattgggag ttggatattt atttatgttt ccattcccat 132060 caatcgataa gcttcagagg aaataagagt gtaagatttt tttgaacaac aggaaaggaa 132120 gattgtagta agatatgatg gcataatggt atatggaaga gttttaaata aaagttagcc 132180 aggcatggtg gcacgcgtct gcagtcccgg ctactcggga aactgaggca ggagaatcgc 132240 ttgaatccgg gaggtgaagg ttgcagtgag ccaagatcgc accactgcat tccggcctgg 132300 gcgacagagt gagactccat ctcaaaaaaa aaaaaaaagg gatgaagagg gatgaggaga 132360 actcaagtta tattagcctg aatggaatag atgagtggag agatgggcat gataggtgag 132420 ttaagaagga aattctttgc ctcagcaacc aactcagatg ctttgtgaat ctaaattatg 132480 tgttgacatt ctagtagacc tagacctggg ataattcact tcagcattat gtaatcttaa 132540 tttttcattt ataaataaga aaatattatg caatacttgc catagttttc taaaagctga 132600 actcagaatg ttaatgaaat taaaatggga aagctatttt gcttagaaga tattttgtta 132660 taaacatttc ttaaatgaag aggctctaga aatatttatt ttatagaatt taaaatatag 132720 ccatcactaa taaaggcagg cataattcca acgatttcat aacacatcag ttattaaatc 132780 taattaatat ataaatgtaa ctaatatgta atttaatata accctaattc aatgtttaac 132840 caactgaagc tggttaaact atgaagaaga atcttcaaat tgaccatttc tttccaagcg 132900 gtaaaggtga tacgggattt tgtctataca tatagagcaa aggagaatta agccaagatt 132960 tagaatttta ggcaaaaagt gtttatagta tcattaagtg gttacatatg taattttact 133020 gttataaata tgactgagtc aatttttttt tcaaagattt tgtcacaatg ggaggcacaa 133080 ggagtttaga cagatgaaga ctctttctag aaatctacct aatctatcac attagacctg 133140 gagataattg gtttgacaat ttcagttgtt tctcagcaaa aaaaataaga aactaaaagt 133200 gttcagatgg cagagaaaaa agtttgaaga ggagagaaag aagaacaaaa agaaaaagga 133260 aacagtagac atataaagaa agcacctaaa ccagtgagat gccccagcta tgcaccagca 133320 gcatggtgga ttacagtaga gtttttctac agaactattg taaatcactg gaaaatagtg 133380 tccaacttat ttgcttaaaa ttttcagtta atgtgctcca gcagcattct gtagacaacg 133440 actctcactc tccccttatt atggaggcag agctgtttct ctttgtcccg ttctctagcc 133500 tgtgatatac tttttattgc tcaagagcta tagaattgta tccatgccta ttgttgtcct 133560 cctgatggga attcatgtaa ttgaaagcgt ttatcctctc ttagtagaaa aattgggcac 133620 ttggaggcaa tgaaaatccc ccacctttgt ccacggcact ggaaatactc ttcagtctgc 133680 tttgttctaa agtttctact tttcccagta taacctgttg aaaatttagt ccttcatgag 133740 aaatacggct ggaatttctt gtggcaggct ggccgccggt gttcatgtag ttacaccttt 133800 tgttgctatt tcaccactga tcaaaaaata aaattgtatt ttttcaaaag aaacatggaa 133860 tgacatgaag aaagtcctta atatatcatg gagtgaaaaa cagcaagttg ccatgcaaat 133920 gtcaaataga tcctatattt ttaaataagc aactgcatat gtatcattta aatatatatg 133980 tttagatatt tgctaaatta tgaaccgcag ttacccctgg ggagtgaggt taggaaaagt 134040 aaagtgagat tttcactttt tacattatac tttttacttt acccacttct gtattgtttg 134100 aatctttaaa atgaacaagt gttattttgt aattaaaaaa aacaacaact gacaaataac 134160 tatggggagt aagaaattac ttggaatgga atatgtttaa ataattaatt tgttgataca 134220 ctgcttgact attattttct tttgctaaat caaaactccc ctgcttttct caggaggttg 134280 ttttgaaaag aagggtaaat gtttctaatg aaatgaacag ctcaaccttc ccacctcctg 134340 cctaaatgcc tcattttggt tttgttacta acaaatgcaa cacgcaaggc tttatggtaa 134400 agcaagcaat gtgggtttct aacagggcag tacacacccg tagttgctgg gaaaccactt 134460 tagtaatggt ggatttattg aattggaaca ggaatggaga tttggaacaa caggaagtga 134520 ggtacctgta ggggtctcag ttgtcctggg cactgtgaca tggcacccaa atgtcatgca 134580 agagtggccc ttccaacata tgcaccaatc tgaatctctc caaagtttct tgattacacc 134640 ataaaacaat gaagagaaca gcattgccaa taacttcagt aataactttt gcatcatatt 134700 acaaagggtg gtgggtggca ctttgtgtgg tcatgggtat gtccaaaaga agggagtggt 134760 ggctagactc agttctcctt tagattttaa ggcaaaattt agcaaactct atggcagatt 134820 cttcaactat ttgtcagaca gacattgtct acttttctgg ggatttatga ttgaggcccc 134880 ttttgagggg agctttgagt gcagtgaggc cagatagaag ggccaagaag gatgggagca 134940 gctgcatgct agtgagcaat tcaaagttgg attgtgctga ctggaaaatc ccggttagca 135000 aatcaactat cacagacttc atattctttt cagatgtgca gcctttataa catatttgta 135060 aatgctaaag gactgctcca cagggaggtt acaaagagaa aagctctggg tggttgtaaa 135120 atggacaaat tgtaaagcct tactagtagt cttaccattc ctctcagctc ataaaaaagt 135180 cctcctcttc atttgactat gcctcaaatc tactgcttct tggattatta aacactcttt 135240 tcctccagga tcttaaaatc tctctgttat aagttgaatt gtgtccccct caaaaatgat 135300 atgttgaggt cttaaacccc agtatcctag aatgtggcct tatttggagg tggggtcttt 135360 acagggttaa tggaattaaa acaaggccac tagggttggc cagtcctttt tataaggaat 135420 ccagtatgac tggtgtcctt attaaaaggg aaaatttgg.a cagagacaca cacataggga 135480 gaaagacgtg atgatgaagg cagagtgggg ggtgatgctt ctataagcca acgaacccca 135540 aagattgcca gcaaaccccc agaagctaca gagaagcatg caacagattt tctatcacag 135600 ccctcaaaca gaaccaaccc tgccaatgct ttgattttgg acttttagcc cctggaactg 135660 tgagacaata catttttgat gtttaagcca cccactttgc ggtgctttgt tccagcaacc 135720 ttgggaaatg attacacttt ctcatctgtg atcacagtga ggccttgtca caatcttcac 135780 tcattccttc tatagaaagc tgaaagccta tggacaagga taattgcctt ggctgacgca 135840 tatgtttgaa ggcagctgct ggcaggcaac cggagacttt accctggtcc tagtggagag 135900 gaagtctata ggaggaggca gggagccttc ctggctggca acccagtgac tcaggttgtt 135960 ttcttactcc aaggatctct gtgcaagtag gaatcctgac atcttctctt tttcacttgt 136020 ttatgagacc ctgcttgttg tagagtaaag agcagaactt aagaatggaa tctaatcacc 136080 ttgaaagaat aatctctcat ctcctctggt ggcaaaggat tgggatgtgg aggttatagt 136140 tggaaaattg attcctaggt caatgtctgt gggtgaattt ctctttgaat tgttatcagc 136200 aatgttatgg tgttcggtgg ctttcaacga aataaacaca cctgacatcc ttagtaaaga 136260 gaaagctttc tgaaataaga gtgagggaca gactgggaca tgatgctttg catgaaatag 136320 gtgttcagta agtatttgta gagttggaag aagttagatt aaacagggct attgtatggc 136380 acattgtcat tagggagtta gagagggctt ctggaaaggt ttggtcagtt ttgacttacc 136440 ttgcaataaa tttggactat aataaccatt tcactagttt gtaaaaggta ctggcaacca 136500 aagatgggga atgccactca atctcacttg ttctactttc aaaagcttgc tttggtcaca 136560 ttgaaaggtt ccatttggtg aagtatagca tatcacagaa aacaatggag tttgggagtt 136620 cgggaacaaa tggttgtttt gtgataatga ataactttga catttcgttt gaagttaagt 136680 tatgttgttt gaacttttct gtccctgaac aaggcatcgt atgccaagaa caggtaacag 136740 tttctggatc actggggact cattagtcat ggatggttag tggtactaca ttgtggtctt 136800 ccttcaatat tgaaagctgt aagaagcttg tgacaaattc ttaccttggc cctcacacag 136860 tactggtagg gctgggatgt tattgctttt gtccaccaac ttccaactgt agaagtctag 136920 gatgatcaca gcagttagag atgatccatt tagatagtct atattaaggg aaagtatcct 136980 tcatccatgg tgttgaacat tcgtaggatt aaaatgaaag aagccaaaac ctttctagat 137040 ctttattggt atatcaacag aattagctga ttaggtatgc agaaaaaact tgggacttga 137100 agacaccatt aaataaataa ctgtacataa ccatacatta aataactgta acaccattat 137160 ataaataact aactataaat aaagagctct acattcaaac tgcttttcac tgcatttgtc 137220 agatttcact tttaatataa ttttagttga cttatccctc atcttacctt aagaagataa 137280 tgagttgagg tggatcttga gccagtgttt agtccaatat agccctttga cttggggaaa 137340 gatcaaactt cgaaatttat atgcatgact cctctctggg caaggactga agtggcagga 137400 gaggtgaaag aaggaatcag gacaaaaagt agatgctaaa aggaaaacag tctgtcccgt 137460 ggagaggaag tacccaaaga aacagaagaa ctccatgatg gagagtaata caagttgaat 137520 atcccttatc caaaatgctt gcgaccagat tgttttggct ttgggattat ttcagatttt 137580 ggaatatttt catacccgct cagtatccct aatctgaaaa tccaaaatct gaaatgcttc 137640 agtgagcatt tcctttcagt gtcatcttgg ccctcaaaaa gttttggatt ttggagcatt 137700 ttgaattttg gatttttgga ttagggatac tcaacctgta ctagcgtgtt aaaacagtcc 137760 tggccaggcg cggtggctga cacctgtaat cccagcactt tgggaagccg aagcgggcgg 137820 atcacaagat cagaagatcg agaccatcct ggctaacacg gtgaaaccct gtctctacta 137880 aaaatacaaa aaaattagct gtgccgtggc gggcgcctgt agtcccagct actcgggagg 137940 ctgaggcagg agaatggcgt gaacccggga ggcggagctt gcagtgagcc gagatcgcgc 138000 cactgcactc cagcctgggt gacaaatcga gactccgtct caaaaaaaaa aaaaaaaatc 138060 caaaaaaccc aaaacagtcc tagaggctac agcctagagc agtgggcaaa gagaagcaga 138120 atctacagaa gatattgttc aaggcctgct atgctaggtc tcagatagca ccagaactgg 138180 gcagggcaag aaacgtcagg cccaggggtc tggtttaacc agaagaggtg ctgaatttta 138240 ggatctgaat agcaagggtt gtggtcagga agtcagtttc atgtaagaat gaaggtgagc 138300 ggatcttaaa tccctgaaag gaggcagaga gaagaatata ggaaatgggt ggataatttt 138360 tgttgcgagt aaaaatgttc aggactttgg aggcaggaag agcaggtaaa gcaagttcga 138420 ggaaagaggc tgggtcagag gggatctgaa agcaggagtc agaaacatcc attggaggcc 138480 tggagaacat aaatgggagg tgaatagact ggggacatag tgcctgggtg gaaagaagct 138540 ggctcgagtc cgaaaccaca ggctgaaata ttaggaaaaa gagacccagg aattcagttc 138600 agaataggag ggatttgtgg ggcaagacca gtggttcttc cagacatttt atcagagata 138660 acaaagcaga ggctgtctgt tctctgccct tggtgtgaga agaaaaggac tgacaggagt 138720 gcttggcagc tcaagccagt tatggagctg tgggtgctag tggctattaa ctaccaggga 138780 attagctctg ctgaatgagt cctcacgcag aggcagggat agaacgttgc attcagaaga 138840 ctggccacgg aggccggctg gggtggctca cgcctgtaat cccagcactt tgggagacca 138900 aggcaggagg atcatctgag gtcaggagtt cgagatcagc ctggtcaaca tggtgaaacc 138960 atgcctctac taaaaataca aaaattagcc aggcttggtg gcgggtgcct gtaatcccaa 139020 ctactttaga ggatgagaga ggagaatcgc ttgaacccgg gaggcagagg ttgcagagag 139080 ccgagatcac gccactgcac tccagcctgg gcaacaaaga gtgaaactcc gtcttaaaaa 139140 aaaaaaaaag gaaaagaaaa gaaaagaaga gtggccacag attggcttgg ctggagaagg 139200 cattttttta tagggagata tacgtgagtt tgacgggatg ggctagcagg cggacattca 139260 ggttttatgg gataggcaaa aaggtacaaa tggtatttgg ggggatgtga atggcatctg 139320 agaaaggttg atccaggctt gtgtggaagc tggctctgag cataaaatgc cagagaaagg 139380 cttgtacaac tctgaggtct tttcttttct tttcttttct tttttttttt gagacagagt 139440 cttgctcttg tcgcccagcc tagagtgtag tagtgtgatc tcggctcact gcaacctctg 139500 cctccctggt tcaagcgatt ctcctgcctc accctcctga ttacctggga ttacaggcac 139560 ctgccaccaa gcctggctaa ttttttgtat ttttagtaga gacacggttt taccatgttg 139620 cccagggtgg tcttgaattc ctgacctcaa gtgatctgcc cacctcagcc tcccaaagtg 139680 ctgagattac aggtgtgagc caccgcacct ggcctatttt ctatttttta accagtccca 139740 agtaagggta ttggttaggg ttgaggcaat ggaaatctga aagaaagggt gagttaggta 139800 tttcactgga cacatgtgga gaatgttggt gatttaatgc agagggaatt gtggttaaaa 139860 aggttaatgt aagaagggtc atgtggggac tggaaatcac aatagagaaa cagaggccag 139920 aatgctgaac tcataggaag tgggtgacca tgggtgatta aaatgtacaa tagggctggg 139980 tatagtggct catgcctgta atcctagcac tttgggggac tgaggcagtt ggatcacctg 140040 aggtcaggag ttcgagacca gcgtggccaa catggggaaa tcccaaccct actaaaaata 140100 caaaaattag ccgggcacgg tggtgcatgc ctgtaacccc agctacgtgg gaggctgagg 140160 caggagaatc gcttgaaccc aggaggcaga gggtgcagtg agccgagact gcgccactgc 140220 acttcagcct ggggaacaag agcgaaactc tgtctcaaaa ataaataaat aaataaataa 140280 aaataaaaaa ttaaaatgta caatagagtg tgtaaaacca ggaaatctac atcttgacac 140340 ttaactaccc aagacgcaat tggttcctcg tctgattttt aaaagaagtt tccactttct 140400 tcggaaaatc acgacagtcc ttgttctcaa tttctttgtt tttaagaaga gaaatgttgt 140460 atgatttcac ttatataaac tacctagaat aggcaaattc acagggatag aaaggtggaa 140520 tggaggtgac caaaggtgga aaggaaggag gattgttcaa tgggtacaac atttctgttt 140580 gggatgatga agaatttctg gagatggaca atggtgatgg ctgcacaaca ctgggaatgt 140640 gcttaatgcc attacttaaa aatggctaaa aatcatgcat tttatgttat gtatatttac 140700 aacttttttt tttttttttg agacagtgtc ttgctctgtc atccaggctg gatggcggtg 140760 gcatgatctc ggctcattgc aacctccacc tcccaggttc tagcaatgct cctgcctcag 140820 cctccccagt agctgggatt acaggtgtgt gccaccatgc ctggctaatt tttgtatttt 140880 gagtagagat ggggttttgc catgttggcc aggctggtct cgaactccta acctcaagtg 140940 atctgcccac ctcagcctcc caaagtgctg ggattacagg tgtgagccac catacctgcc 141000 atatttacaa cttttaaatc ataggaaaga aaccactaat gctttgacaa ctgtgagatt 141060 ttgaatctca actgtgagat tatggtttgt ggaaatttct ttatgtgatt aaaaaagata 141120 ctttttttgc caaaaaattt gttttcgctg tcatgcaagt tctcctgggt aagcaccttt 141180 ggtcttgttt tgtgcatagc atacagagca gcaattgtat attgctaaag aaagcctgtt 141240 ttttttttct tgttaattta tttaagttcc ttgtagattc tggatattac cctttgtcag 141300 atggatggat tacaaaattt ttctcccatt ctgtaggttg cctgttcact ctgatgatag 141360 tttcttttgc tgtgcagaag ctctttagtt taattagatc ccatttgtca attttggctt 141420 ttgttgccat tgcttttgtg ttttagtcat gaagtctttg cctatgctta tgtcctgaat 141480 ggtattgcct aggttttctt ctagggtttt tatggtttta ggtcttatgt ttaaatcttt 141540 aatctgtctt gagttaattt ttgtataagg tataaggaag gggtccagtt tcagttttct 141600 gcatatggct agccagtttt cccaatacca tttattaaac ggggaatcct ttccccataa 141660 ggatatgaac agacactttt caaaagaaga catttatgca gccaacaaac atatgaaaaa 141720 aacctcatta tcactggtca ttagagaaat gaaaatcaaa accacaatga gatactatct 141780 cacactagtc agaatggttg ttattaaaaa gtaaaaatat aacaggtact ggtgaagttg 141840 cagagaaata ggaacacata tactgttggt gggagtgtaa attatttcaa ccattgtgga 141900 agacagtgtg gcgattcctc aaggatctag aactagaaat gctatttgac ccagcaatcc 141960 cattactggg tatataccca aaggattata aatcattcta ctataaagac acatgcacac 142020 gtatgtttat tgcagcacta ttcacaatag caaagacttg gaaccaaccc aaatgtccat 142080 caatgtttga ccgaataaag aaaatgtggc gcgtgaaccc gggaggcgga gcttgcagtg 142140 agccgagatc ccgccactgc actccaccct gggcgacaga gcgagactcc gtctcaaaaa 142200 aaaaaaaaaa aaaaaaggaa atgtggcaca tatacaccat ggaatactat gcagccataa 142260 aaaagaatga gtttatgtcc tttgcaggga catggatgaa gctggaaacc atcattctca 142320 gcaaactaac acagaaacag aaaaccaaac actgcatgtt ctcactcaca agtgggtgag 142380 ttccataatg agtgggtgag ttccacaagt tccacaagtt tcacaagtgg gtgagttcca 142440 caagttctat aatgagaaca tatttgcaca gggaggggaa catcacacac cagggcctgt 142500 cggggggttg ggggtcaagg ggaagaatag cattaggaga aatatctaat gtagatgacg 142560 agttgatggg tgcagcaaac caccatgaca catgtatacc tatgtaacga acctgcacat 142620 tctgcacatg tatcccagaa cttaaggtat aataaaaaag aaaaagaaaa agaaaaaaaa 142680 ccctgccttt tgctttggca gtcatcattc cttccatctt ctcatgtttt cttatttaac 142740 tcagtagttc tcaattgaca gcacaaaaga attactcagg agagttcaaa aaacacctat 142800 ggctgactcc cccaagattc tgatttcctt ggtgtggggt agcctgggca tgaatattat 142860 ttaaaaactc ctctcatgat tctaaggtgg tagccaggat tgaaaaatgc tggactttca 142920 agtttttgtt ttctttcttt tcttccaaaa aggtgaagcc ctgacttggg aagaattcaa 142980 atgcgagtta ggcttatgtt tgatgtcact gattcctaaa gatcagtcca ttgattctcc 143040 tcattctcag gagagcattt ggtgtcaaaa tcacagccca aaactcttac cctagttcag 143100 catctaact,c tcttcagtcc tgaaactgtt tttgtcactc tgtccatata atcacagtgt 143160 tgtagaaatt tccagctaaa cattgtctca aactttttgt acctagtttt aaatcaaaat 143220 gtagtacaaa attattaaat acaacgggct ttaaaatatt gagattttgg tgatcttttt 143280 tgtagaatgc aaatactaat gaaactaatt ttttcttttg ctttcaaact atacatttct 143340 tctaaaactt ctttcaaagt agaatttcat aagtggaaga acccttgaag attattttaa 143400 tagaattcct taattttaga gatgaggaaa ctgagactca aatatattaa ttaatttatc 143460 taagtgtctt agtccatttt gtgctgctat aagagagcat acttgagact gggtaattta 143520 tattgaacag aaatttattg gctcacgatt ctggaggttg gaaagtctga tatcaaggtg 143580 ctggcatctg gcaagggcct tctggatgtg tctacatggt ggaaggcgga agggccaaga 143640 ggtaaaatgt ggctgaattt gtgcttctat tatggcatga atcccatcca tgaaggtgga 143700 ggcctcatgg cctaatcatt tctcacaggc accacctttt aatactgtta caatggcaat 143760 tacatttcaa catgagcttt ggaggagaca aaaattcaaa ccatagcact gaagttatac 143820 aactagttaa gaagagagaa cctgagactg aaaccctgat gttttgactc ccaatccaga 143880 atgtttgatc ccctacatcc caccgcttcg ctgtttccat tcctttctcc tgttttcatc 143940 tattttcact aagagggcaa gatatttgct aactgctaca cacccaaatt gtataccagg 144000 caggtcagaa tgactctaac attttattta atgaggcaga ataaaatatc ttcctactaa 144060 acagtgacaa gtgtttgttt taaaaaaaag tattgtgcac aaaaaaaaaa actactttca 144120 gcataggcta tattttacaa aataaattta cagtgggaac cacagagcta tttagagtgt 144180 ctggtataaa tgattttatt gttactctgc ttttttgaaa aagaagtgtg tgaagcagag 144240 gtaagtagta ctgaggaaat aataactaaa atactaagga cgagatgcac atgggaaaag 144300 ttcttcatta actgtaacgt ggtggagaaa tgtatgttct agacaggtca atcatggaat 144360 tagaaaaagt tgggatcttg gattgttgag ttcaaatcct tagttttaaa actgaagact 144420 tcaaattttg attgactaca gatcaatcaa caacctgttg gagttggaaa cttgaatctg 144480 attctccatc cttgtttggg atatgaaaaa aaggaagaca tttaagagta tttttttatt 144540 tatgaaacta atgtatcatc atgtaaaaat atgaaataga aagtgttaaa aattataaag 144600 aaacaagaat gtgtttttat aatctcattt tcttacttta cgtaagatgg taagtctatt 144660 ttggaattaa ttgtttttaa gaatagtatt ataattcaat gttagataaa gatttattta 144720 accctttccc ctttaacaag tgtttgggtt ttttaagttt gcttccaagt tttcggtaaa 144780 tgatgcttca atggatatct ctgtttgtta tcttagtatg tatcttagat taattcctca 144840 agataaatat ttaaaggtat ggtatatgta atattcttga tacatacttc catatttccc 144900 tccagaaatg tgtgcaccta tttaaatgtg tgtggaggtg cacattttaa cattcctcat 144960 tattctggct gccttctata aatcaagcta ccctggtatt atggctctcc tccagaaatc 145020 taatcattta aacacatgaa gtgaatgtgt gaatggacaa ttaagtgctg gccaaatgtg 145080 gaaaaatgtt aagtgtaaat caaaggcaac tgaaacgtta aatacacctt ccattcctcc 145140 gccacatcca tgctctttcc catactccac atctgctcac taaaattgga aataaactgt 145200 aatgaaccag aaatgtctga cttactggtg tgtttaggtg agttttattg aaacactgac 145260 tgaccccacc actctaatac catcacctta atgtttgtat cttagattaa ttcctcaaga 145320 taaatattta aacgtatggt atatgtaata ttattgatac atacttccat agttccagca 145380 accacttgtc atcctgtgtc ccccaaggac actgcaatga ctgtcacagt gacttgtgct 145440 cggttgagga gcaagtggtg gctgaaatcc agccccagtc agctccccaa accaaaccac 145500 ttttacacag tgtctatgtg taaagttggt ttgctttggg gagattgatc cccataaggg 145560 atcagcacca gccctgtgtc tatctcttct tctcctgggg agatgttggc tattttgcta 145620 gctggagaag ggcacgcaat ttgttgtcag gagacaggtt cttaagttcc attggtacat 145680 gcagggcaag acaggcagag ggaatctgtc actgctacag gcacttcata agcccaggta 145740 tagacgataa ttgaaatgga ttgatttcat agtagtctaa tacagagtgg tgctacaatc 145800 tctgtatgtg cctatttaga aaataacttc atatttaatt cttcacttta cataaaagat 145860 atgtgtatta aatacacctt ttattttaga acagttttag attttagatt taaattggga 145920 agacagtaga gatttcctat ctaacccaca cccagctccc gtattagtaa ctaacatctt 145980 gcattagtat ggtatattac aattaatgaa ccaatactgt tattttatta ttaacttaat 146040 tcatgcttta ttccagttcc cttagtttct acccaatgtc cgttttctat tccagtatcc 146100 catccagaat accacattac attctgttgc catgagtttc tcagattttc catgtttttg 146160 atgaccttga cagttttgag gaatactggt caagcgtttt gtagaatgta cctcaactga 146220 gatttgtctc atgtttttct catgattaac ttggtattac aggtgttttg agaggaagac 146280 cacagaaatg aaatgccatt cttattacat catatcaagc atatattcta acaatatgat 146340 ttatgacaac taatgttgac cttggccaac tggctggggt agtatttgtc tagtttctcc 146400 tctgtaaagt ccttccttac cccctttcca cactgtactc tttgaaggaa gtcactctgc 146460 acagctcaca atgaaggagt ggggagctat gcttcccctc tttgagagtt gtgtatgtac 146520 acaaatt'att tggaattctt ctgcatggga gatttgtcta ttctccccta tttatttatt 146580 tattgaatta tttatatcag tatagcttca tggatattta ttttagactt tgggctaaaa 146640 aacaatacta ctttatttta ttgttcaaat tgttccagct tctagaagtt atatttttat 146700 agagaagata tgtttttaaa tataaaaaca ctttaaaaaa atgaagtcaa cagaataaaa 146760 tatttttaaa ttttccttaa tgggtgttct ttgtaccatg tttccatcaa ataacattct 146820 gattgaaatc taaaaattag tgtttgtatt ttaagtgcta agatcagaga aaaagtcaca 146880 agtttcccag actgagttag gcaacatgtg actcagagtt ataaggaata cctttcccat 146940 tcctgcattg caaccagtgt tacagttaca gaagtgacta gtgtaacttc ccctcctggg 147000 ttccagctgc caggacttac tttagttctt ttaacatatc cttcccacca aagataagtt 147060 ttttgaacag tttccccatg agtgctcccc ccaccccaac ttgtgccttt tattcatgtc 147120 tttataatat ttattggttg gctggtgcaa ttgcagaggt tggcaagtct gaaatttgta 147180 gagcaggcca gcaggctgga aactcaggca ggagttaatg ctacattcta gggacatttt 147240 tttcttctct ggaaacttca gtttttgctg acaagctttt caactgattg ggcaaggccc 147300 acccatgtta tgagggggaa ctgtacaatc agttgtcatt taagccaatc agtcaactga 147360 tggttggtgt caaccatatc ttcagaatac cttcactaca acatctagac tcatgtttga 147420 ttaaataact gggtgccata accttggcaa gttgacacct gaaattggcc atcacagtga 147480 ccaagacaga aagtgtaatg agcaggaaag caccccagtg acaggacagt gaaagatgag 147540 cctggagacg gggagtggga ggagtgcttt gacagaatgg tcaagagaga tggcatctgg 147600 gtggagatat gaaaagatgt aaaagaaggg aaggaaccag ttctgccaag aatttatact 147660 tctacaattt cccagatgat attgatgctg ttgccctgga gcccatactt tgagaaccac 147720 agagtaaggg tatacctctg ttattatcca atgaatcctg agaaagccca ttaatatctc 147780 agtgtgacca ttttttctca aaattcctac ctcacatgct ctgaggggct ggcagctttt 147840 ctgggaaggt aaaatctcaa gagggaggct ttgaatgatt ccaggacaaa gtgaagataa 147900 catactaccg caggtaaagt ctaggtttac ttggaggtgg ctcaaaacac aaatcctgtc 147960 aattttaatg acacatgagg ccatgactct gggtcgtcac tctgggcaag ccattgaacc 148020 cattagaacc tcagttttcc catctgtaca aaaagaggtg ataatgggta agcaatcctg 148080 gactgcattg tggtagtgcc atcaagcaat gagatttgtc aggaaggaag tgtgattcaa 148140 gtacaggcac tttgttatct gatgcctgtg tgttgagtgt gaaggagggg ttaattattc 148200 ctctattcat gtcctttcta ttctcaaaat ttcttatgtg ggaggaaaat cttggtgcgg 148260 taaagatcac agccaccctg gggtcagaca gaggactgtg tcaagcttgg taagcgtcac 148320 cattcaggtg agtgggaaag agactgaacc ccattaaacc agatgacctc caaggccctg 148380 cccgccccaa catctgaggg ctgcctggct tgtctgcaca gggtgctcac agtactcctg 148440 actttgacat aattttaaca ttaaagtcac aaggatgccc tggatcacaa ctgctggaga 148500 agagatggta gtgggatttg tttcccagga gactttctga ttctagtggg gccaggacca 148560 acctccaaaa taagaggtct ttgcaactgc tgcgagcctc ctggcacctc taccctctag 148620 cgagaggctg cctctcctgc cccaccccgg tttcaagcac gctgcagggc aggaactcac 148680 tgtgctggca aaggtgagct ggagacctgg cacggcataa agctttttta actgaccctt 148740 tttaatttca tcttccctgg acttaatcta gagagtcatt gatgaaacaa acatgccatt 148800 ttctcccgat ttcacgcttt taaatgtcaa caacaaacaa acgtttatat acacaaatgt 148860 tgctgaagga gacttttggc tttagacaag ggtaaaaact gaacctctta gtgtgacttt 148920 ggttgatttt ttaaaaaatc tgtaatttga catataaaga attattaaga cttttttttt 148980 tttttctggt aggcattgaa tgtgtccttg aaaagaaaaa aaatatgctc agtttcaatc 149040 ttctgtctat gggtagctag ttatcccagc cttagttatt gaataaggag tcttttcacc 149100 attgcttatt ttattttact ttttgagatg gggtcttgct ctgtcaccca ggctggagtg 149160 cagtggcacg attttggctc actgcaacct ccgcctccca ggttcaagcg attcccctgc 149220 ctcagtctcc caagtagttt ggattacaca tgtgcaccac cacacctggc taatttttgt 149280 attttgatag agacagggtt tcaccaagtt ggccaggctg gtcgtgaact cctgacctca 149340 gttgatccac cccacttggc ctcccaaagt actgggatta caggcatgag ccaccgtgcc 149400 tggctgcatt gcttattttt gtcagctttg tcaaagatca gatagttgta ggtgtgtggt 149460 cttatttctg ggctctctat tctgttccat cagtctatgt gtctgtttgt gtatcagtgc 149520 catgttgttt tggttattgt agccctgtag tatggtttga agttggataa catgatgctt 149580 ccagctttgt tctttttgct tagattgtct tggctatttg ggatcttttt ttggttccat 149640 atgaatttta aaatagtttt tttctagttc tgtgaagtat gtcactggta gtttgatagg 149700 aatagcattg aatctataag ttgctttgag cagtatggcc cttttattga tattgattct 149760 tcctattcat gagcatggaa tgttttttcc atttgtttgt gtcatctctg atttatttga 149820 gcagggtttt gtagttcttc ttgtagagaa tttcacctgc cgggttagct gtattcctag 149880 gtgttttatt tcttttgtgg caattgtgaa tgggattgtg ttcctgattt ggctctttgc 149940 ttgactattt ttggtgtata ggaaggctaa gtgatttctg tatgttgatt ttgtgtcctg 150000 agagtttgct gaagttgttt atcagctgaa ggagcttttg ggccaacact atggggtttt 150060 ctaaatatag ggacatgtca tctgcaaata gggatagttt gactacctct ctttctattt 150120 ggatgtgcta tatttctttc tcttgcctga ttgctctggc caggacttct aatactatgt 150180 tgaataggag tggtgagaga cggcatcatt ttcttgtaac tggacccctt ccttacacca 150240 tatataaaaa ctaactcaag atggattaaa gacttaaatg aaaagcccaa aactctaaga 150300 accctggaag actactgagg caataccatc ttagacatag gaatgggcaa aaatttcatg 150360 atgaagatga caaaagcaat tgcaacaaaa gcagaaattg agaaatgaga tctaattata 150420 ctaaagagct tctatataac aaataaaact atcaacagag taaacagaca acctacagaa 150480 tgggagaaaa tttttgcaaa ctatgcgtct aaccaaggtc tactatccag catctataag 150540 gaacttaaat ttacaagaaa aaaacaacct cattaaaaag caggcaaagg gcatgaacag 150600 acacttttca acagaagaca tacatgtggc caacaagcat atgaaaaaag ctcagcatca 150660 ctgatcatta gagaaatgca aatcaaaacc acaatgagat accatctcac accaatcaga 150720 gtggttgtta ttaaaaagtc aaaatataat agatgctggt gaagttgcag agaaatggga 150780 acacttatat aatgttggtg ggagtgtaaa ttagttcaat cattgtgaaa aacagtatga 150840 tgattcctca aagacctaaa aatacaacta ccattcaacc tagcaattcc atgactgggt 150900 atatacccaa atggatataa attgttctat cataacgaca catacatgca tatgttcatt 150960 gcagcactat tcacaatagc aaagacatgg aatcaatcta aatgcccatt gatggtagac 151020 tggataaaga aaatgtggta catatacatg atagaatact atgcagccac caaaaagaat 151080 gagatcatgt ccttttcagg aacatggaag gagctggagg ccattatcct tagcaaacta 151140 atgcaggaac agaaaaccta atactgcatg ttctcacttg taagtgggag ctacatgatt 151200 agaattcatg gacacataga gaggaataac agacactggg gcctatcaga gggtggaggg 151260 tggaaggagg gagaggatca ggaaaaataa ctaatgggta ctaggcttaa tacctgggtg 151320 atgaaataat ctgtacaaca aacccccatg acacaagttt acctatgaaa caaacctgca 151380 cgtgtacccc tgaacttaaa ataaaagtta aaaaaaaacc ccaaaaggtg aagtgttggt 151440 gaggatgtgg agaaaaggga acccttatta cacaattagt gggaatgtaa attagtgcag 151500 ccattttgga aaatagccaa ggaaaatttt tttattgagt cagatgatgt taccttacag 151560 caatagcttc caaaggggat gtcctgaata gtagaacttc tctttacatt ttcaaacctc 151620 tttttacttt ctgattttga gtttcataat gaacatgtat caatacaata gcacactatg 151680 tattttataa atgaacaaat atgggaaaaa taagatatgt tcactttcct ggctgaccct 151740 agttagtcta aactatcagt tataggatcc atttgttcta aatattgaag gcattgttgg 151800 gggcagtggg gagggtgaat gatagacgca aattccttga aggaatagaa atgtgactac 151860 tatgctgaaa agcaggaccc atggagtata aatagagtat tcactgtgct tttacatttg 151920 tttccctgga ggaaacgtaa aacaaatctc aagactttct ggtcatttct aagtcataaa 151980 cggccactag tcactaattt atataaccag cccaatgtaa atatcaaacg tgtgagtttc 152040 atttcatctc acaactcctc ccctttccca gttctggccc catacaagcc cgctgcctgc 152100 agtgggatgg aggaagatgg tttctttttc cttccctccc atttatttgt ctttgtgcat 152160 cttaaagcca gcctgaattt ctactcccta caaggttgaa gtgggtgggt caggaggtgg 152220 ggagagagct aaggaacgtt ggcgtgagtt gttgttgtgt ttgagtctgg aaggtgttgg 152280 gagctgcccc ccctcactca gggcctcatt tatgggctct tcggataacc cccatgctct 152340 agcactggga tcttgccctg tggatccctc catgcaggtg gcaccacacc ctctggctgg 152400 ttgtgatggg ccttctcagc tttgagaatt agcaacacac ttcctattga agtcatctgt 152460 ccctgtgggt agcccacttg ggctggatgc aaactgaacc tgcactgatt ctctcttgca 152520 tgtggcccac atgagctcca ttttcctccg caaactttgg gtgcacagcc ccacgacgac 152580 acagacagct tttcttgcgt gccaccaaag tggtgggcca gagttctctt atccttaaga 152640 ttttcaggtg tcaccacgtc caccaattct tggcagacat gaatcaatac ttccaagggt 152700 tttggtgaag cccttttccc tgggctgcag atgagggcag ccatgtcctg tctcttccac 152760 ctgttgtgga aggggttcat ggcaaacatc ttcgaacatc ctctaatttc ccaattcttg 152820 atcctttcgt atgcttgatg tgtgagagag aagagagaag agagaaacgc ttcttgctgc 152880 tttttttttt tttaatccca catggtgacc ttagacttta ctttggaatg tgccatttaa 152940 tatctgggga cctcagccca acacagggac taaatagctc tgttttaaca ttttgtttat 153000 atacacacat tcatttgacc aaatgaacat gttccagctt ttttaaccct tgcccagcaa 153060 atcacttaat tcagttaata aaaagactaa aataatgagg ccaaccttat tggccacgaa 153120 catctgttgg gtattcctaa attgatccct gagttaatct ccacctttaa ggagtttata 153180 attttatttg gcaaattgat gttataatga aattcctggg catgaaaaaa acctaactat 153240 gtaatgttgg atagtgtgag ttttcccaaa agtaccagta taagcaataa atgctataac 153300 tgatcatgaa ttgtttacag tgattgagta gaatcattgc~atctggtcat gaatagaaag 153360 agcacctccc agacggctgg tctgctgcca ggtgtgcagt tttaggggtc tccacactca 153420 ttctattcac atcttttgac tttatcagct gtgtggtgga tctgtggtta caggctgatg 153480 tggtttggct gtgacctcgc tcaaatctca tcttgaattg tagctcccat aattcccacg 153540 tgtcatggga gggatccagt cggaggtaat tgaatcacgg gggcgggtct ctcccgtgct 153600 gttctcgtgg taatgaataa gtctcacgag atctgatggt tttataaatg ggagttctct 153660 ttcacgagct ctcttgcctg ccgccatgta agaagcgcct ttgctcttcc tttgtcttct 153720 gccatgattg tgaggccttc ccagccatgt ggaactgtga gtccattaaa cctctttcct 153780 ttataaatta cccagtcttg gatatgtctt tattagcagt gtgaaaatgg actaatacat 153840 gagccacatt gttacagagt ttctgaaggt cattaagaga agtccatgct gtgggctgag 153900 ctgggactca agaatctcaa aggagagcca gtgcaatcaa cacgaagggc ccatcttgaa 153960 ctcctaaggc agggcagagc tgggtcttat ggagacatgt ggctttcagg taatccagtg 154020 agcacctgtg ttttcctcct ataattcttg aggaatggat att.gttctat atttcagata 154080 gtataataat aaatacctgg aaaatactgt atgaccccaa acaacaggta aaatgtcaaa 154140 aaaaaccttt tatctacaca aagttagaca acaaattcaa ataatcttat ccgtttattc 154200 attctcttcc aatacaatca tgtatcattt aatgatgggg atgagtttgg aggaaggtgt 154260 tgttaggaga ttttgtcatg gtgcaaacat catcaagtat acttatgcca,acctcagtgg 154320 tatagcctac tacacatcta ggcttggata gtacagcctg ttgctcctag gctataaacc 154380 tgtacagcat gttactgtcc tgaatactgc ggcaactata aaataatggt aagtatttgt 154440 atatctaaac acacttcaac atagaaaagg cacagtgaaa atatggtata agagataaaa 154500 aatggtccac ctgtacaagg cacttgccag aatggagctt gcaggactgg aagttgctct 154560 gggagagtca gcgaatggct gatgagtgaa tgtgaaggcc tagggcatta ttgtatgcta 154620 ctgtaaactt tataaacact gtgcacttag gatacactaa atttattaaa aatttttctt 154680 tcttcaatca ataataagtg aacattagct tactgtattt ttttttttac tttataaact 154740 ttaatttttt tttagagata gggtcttgct ctgttggcca ggctggagtg cagtggcaca 154800 atcatagttc actacaacat tgaactccta ggcttaagca atcctcacac ctcagcctcc 154860 tgggtagctg gtactgcaga catacactac tgcacccagc taatttttaa attttttgta 154920 gcgactgggt tctgctatgt tgaccaggct ggtcttgaac tcctgactca agcaatcctc 154980 ctgcctcagc ctcccagcat gctgggatta taggtgtgag ctactgcact tggcctaaac 155040 tctaattttt aaaatcttct tgactgtttt ataataacac ttagtttgaa acacaaacac 155100 attgtacagc tgtacaaaaa tgttttcttt ctttacatcc ttattctata agcttttttc 155160 tgtttaagtt tttaaaaatg ttttagtctg agtgcggggg ctcacgcctg taatcctagc 155220 actttgggag gccgaggcgg gcagatcatg aggtcaggag atcgagacca tcctggctaa 155280 cacggtgaaa ccctgtctct actaaaaata caaaaaaatt agccgggcat ggtggtgtgc 155340 acctgcagtc ccagctactc gggcggctga ggcaggagaa tggcatgaac ccaggaggcg 155400 gagcttgcag tgagctgagt ttgcaccact gcactccagc ctgggcgaca gaacgagact 155460 ctatctcaaa aaaaaaaaaa aaagttttaa acctttttaa aaaatcaaag tcacagacac 155520 atgcattagc ctaggcctac ccagggtcag gatcatcaat gtcactgtct tccccttcca 155580 catcttgtcc cactggaaag tcctcaggga cagtaacacc cctggagctg tcatctcctc 155640 tgataataat atgggcttct ggaagacctc cagaaggacc tgcctcctgc ctaatgctgt 155700 tttacaggta atattttttt ctagtagaag gagtacacta aaataatgat aaaaactgta 155760 gtaaagtaaa tatataaatt agtcatatag tcatttatta tcataatcat tatgtactgt 155820 acataattgt attgccagac ttttatacaa ctggtggcac aataggtttg cttacaccag 155880 catcaccaca cacgtgagta atgtgttgtg ctgtgacatt aggacagcta caatgtcagg 155940 aggcaatagg aatttttcag ctccattata accttacaag accactgtca tatctgcagt 156000 acaaaacatc attatgtggt gcatgactat atttactgag caccaaatat gtgcccaggc 156060 agtgtgctag gggctggaag tgacctcgaa gtctagtaaa aaaaccacaa cagtaaaaca 156120 atatgtgtaa ttcaaagtga catatcctgt aatagctggt tgcctacctg ctccccagag 156180 agaaattaaa cctgcttgct taacttatta attttaaatt catgtacttg caatataaaa 156240 acatatttta aaacatatta ggagaatagg atgtcttctg tagtgatagg ctatttaatg 156300 ttctcttcac tgtgttctaa aaaaatcgtg atcgagtgta acaaaatggg gaagggaggg 156360 ttagcataaa ctctcctttg atgtctatgc tgtggatcag aaaactcatg aatatgttaa 156420 caactggatt ttctcctaat atgaaaacaa ctctttggct tcttttgcaa atgtgaaaag 156480 ttaaccataa atataagacc ttttctttcc actaaaacta gggcacaatc attgttttcc 156540 tcttattgga gtccaaatgg gtttcca'taa tcttcagatc tagtagttgt atttatcgat 156600 gacataaatg gaaagactaa aagataaagt taagaaaaat tcagtggttt atggcttcaa 156660 ctctactgat aattttgtat ataaagggat cctaaattag actgtacaca catttgccat 156720 ttgagttcca agcattttag caatagatca aaagatctta atgcctgata gttgaaagat 156780 tacttccaaa ttttttttat ttttgtgaaa gccaaagtcc ttttctcaac atgacagtca 156840 caattttgtc aacaatctac ccgttattta caaaggttta aaatctgata atagatattt 156900 acttggacta tacaatgtct cagtgggagc aatagatggt ttacaatggg tctgaaaatg 156960 ttgcaaatag tcaaaactgg cattgcaaca attcatttcc aaaaacacac tagactcgtg 157020 agtttttggt ttttaagttt atatagcttg cttctggtgt taacgttctc cacacctaag 157080 gtgccaacat gcatggctac tctttgtccc cagagaaaca ttgctaattt gtaccaaatt 157140 tcaatgatgt gtcaagcaaa ctgtcatttt ggaatgatag taagcacgcc ttcaacaaca 157200 gctctttatt tcgtccaaaa cctttgggcc acacaattct gacctttatc acactctcag 157260 tacctttcat tcattattag cttgtgcttc tggggccccg ggaattgtca actatggtca 157320 aaataatgga gcaaaatagc aagaagaact tgcgtttcct atatcattgc atttaatctt 157380 tgcaacaact ctttgaggtg gaaattgccc caattttata gattcagctt aaagaccttg 157440 atttgtccaa~gatcacacaa ccactaggag gtataatgtt caaataaaaa tgtctgaaaa 157500 gagctgggtg tggtgggtca tgcctgtaat cccagcacgt tgggaggctg aggtgggggg 157560 atcacctgag gtcaggagtt tgaaaccagc ctgggcaaca tggcgaaacc ccgcctctac 157620 taaaaataca aaaattagct gggtgtggtg gtgcatacat gtaatcccag ctacttggga 157680 gactgaggca cgagaattga atgaaccctg tgaggcagag gttgcagtga gctgaaatcg 157740 caccactgca ctccagccca ggcggcagag tgagactcca tctcaaaaaa aaaaaaaaaa 157800 aaaaatctgg aaagatagat tacttggcct caatcatagt aaaaaagtgt cagttaaaac 157860 tgcatggaga gatactattt tgaaaagcat caaaaagttc gaagatccat catatttgta 157920 aggctatggg agtttaaatt gtacaggcgt tatgaaggca tttggttaca tctaccaaaa 157980 ttaacaccgc acatatttac tcatgtgcaa attgacttat gtacaaattt actaattgta 158040 gcattgttga taattgtaaa atattaggaa cacacctaat tgccattaat ggggaactgg 158100 taaaataaac tagaattcct ctgtgcatgg aatactatgc agtcatcaaa aatgacgaag 158160 atgtggccgg gtgcggtggc tcacgcctgt aatccagcac tttgggaggc cgaggtgggc 158220 ggatcatgag gtcaggagat caaaatcatc ctggctaaca cagtgaaacc ccatctcttc 158280 taaaaatacc aaaaaaaaaa accaaaacac aattagccgg gcatggtggc gggtgcctgt 158340 agtcccagct actcgggagg ctgaggcagg agaatggcgt gaacccagga ggcggagctt 158400 gcagtgagcc gagattgggc cactgcacgc cagcctgggt gacagagtga gactccgtct 158460 caaaaaaaaa aaaaagaaaa cagaacaaaa caaaaaatga ggaagatgtt tatttagtga 158520 aatagaaaga tatccaagat atgttgctaa taaaaacaaa aaaaaacaca tggcacaaat 158580 aaggtgcatg ttactatctg ggtcttttaa aaaggggtgt ggaagaatgt gtgtgtgtgt 158640 tttcttcaat atgtgtagaa tatctctgga aggaaattta aaaactggcg acctcagctg 158700 tctccagaga gggaaaattg gtgggttgca ggcagaggtg aaagattttc ccactatgac 158760 tttgaaaata tcttttcaag tttaaagcac caactgcatt atatatcaag agtaaataaa 158820 atacaatttt aaaatgaaat ggcatttgta aaacacttga tagatgttca gttaacagcc 158880 atcaggattt attagtaatt gtggtttttg ccattaaaag taattttaaa agtaatggtt 158940 ttgccattag ttttaatgac aaaaaccaca attagttttg caccaaccaa ataccttacg 159000 cttctggtga gggctcagaa gagagatcat aagtaaagaa cactagaaag gatgtttaat 159060 attatttccc tttttaaaac tggtggttgt caaagcagtc tgaaggaaaa gtctccagaa 159120 ctggtgatca tgttcacata gcagtagttt ggagtcaaca gaaatcatct tcaccatcaa 159180 cctagctccg aaagaggccg agccaatcta gttcccttgg ccaaccacct tcctcccaac 159240 ctatacgttg.gggtgatcca ctacaaagct ctcaagagta tacacaatgc tactacagtc 159300 attgtgagtg agatctgaag ggagcatagg ctaggactca tgagattata gttcagagtt 159360 tctactgtaa ttcctttcag caaagtgaag agcatgtcac tggggttaca agtgttactg 159420 gctacttagc tagtgctaga ttacccaaaa aagtaaccgg gtctcatggg cagaggtggg 159480 aaagtctgtt aagcacatag tgaaggatga atctgggctg attggaaaac atttgtttga 159540 atgaatgtag catttatttc caagaataag catgtccaga ctgattgagc aagagttata 159600 tttagttcag tcgttctgat gtccattttg agaaagctaa accagcatgc cagaaggggt 159660 caaagatcac attttgcatg aaagaaaagc caatggaaat gggactgtta gacttaaact 159720 tttgggtatt ttacactagg acttagaaag gactttattc ctttttatat tttctgtctc 159780 tagcattgca agtagcaaca gagtgaattt ttggtatttg gaatactctc tcctcatttt 159840 tcctttggga aaatcattag cctatttgat ttgactgctg ttgaatatgt tccatgacat 159900 ccataagtct accttcaggc attgcttgtg cttgtttatt acatatgatt aattgcttac 159960 ataagaacaa tgatgatccc tgcaggggga ggagggatgt gaagaacaac ttacttaggc 160020 attccagggg ctaacaaggg atggtttgat acagctcatt cttgttttga atcttcatgc 160080 ctcttgagaa agaaaggagc agaatatgct ttgcatggcg ctatgtcctc ccaggtccta 160140 tgttttcttt tttgtatttt atcagttctc tacgtaagga ttttctcagt ttaatataat 160200 ttccattttt aaaattcaaa tacctacata tgaggtggga aaggaaatta atcagatgca 160260 ttcctataaa ctgcagactt aaattaagac cttgagcaaa ctggcatttt gatgacttga 160320 ggattcagtg ggtgggacag ttgtacatct tgaactctgg gtacactgcc gaaagcgagg 160380 cctgagaagg ctgtgttgag gaaggagtgt cagtgactga gcctgttgga aagggaggct 160440 gagagagatg aggacacata cgtaagggag aacacattgg ctggccaggt gttttttttt 160500 ctgctgtggg ttttgattag aattctgggt ctcattaagt gacccatgtt gaatagagat 160560 tggatagggc tggcttggaa agccctcagt gaagagatga actcgacagc atgataagga 160620 agtagatgca aattcaggac aggaaatgga gaagggcagg gtgagaaaag ggaacaaatt 160680 tacaatttgt gccattagcc atttattaga atttggtata agaatttgtg gaattattgt 160740 ttccttctgg atttatggta caaaaaccta ctgacaagtg tcactaaaag tgatgtcttt 160800 attcaattat caaccaaaag ttttacacta aattcttttg agtctttgaa tgtttatgtt 160860 atttccaata atctttccag ttcttttaaa aggctgattt ttaggaaggc tagcactttt 160920 tgatcacaga atagtttccc caatgagtat gatagcttca atatctttca aattagattc 160980 ttttggaaat tgaactgtgt caaatcaaat aaaacaagta gggttttttg ctgttattgt 161040 tgaaggtatt atatttgcaa gtttgtacaa tttagcagat ataggaaagg tctaattttt 161100 ttctttagct agaatgttca gttcaaatta tacagatata agcatgagac ctaaatgaac 161160 tctcttctga tgagtaatga atggaaggta gattactcat ttcttcatgt tcaaaaaaca 161220 aattatgtct ctgaacacag ttgtttcaat gtttttaact tatgttctca agctcttccc 161280 atttctttat cagtgatttg ggaatgtgaa ggcagtcttg ttgtagtttc caaagattta 161340 ggcatttttg agactctaag aatagttcat taagccacca attagtccct taattttatt 161400 taagctgagc aagcaaggcc cacactaaaa gtcagaagaa tgcggcatct acccagagag 161460 gctgagggtg ggatagaaag aaggaggcac tccaagtttg gagacacaga ttctattcca 161520 gttcctcaag ctgcatgacc ttcagcaagt ctcctgtgtt gttcaggacc ccagtttctt 161580 tataagtgca atgaagagtt tggactcaac aacttataaa gaaacattca ttctaattgt 161640 ctattgtgtg aaaagaatgt gagttgttct agaaaatatg aatctgctcc ccaattgtcc 161700 tccaagctct ggttctaaat ttcagttatt atagttactt ctttcgaggt aaatcattga 161760 ggaagtcagt taatgcagag atgatgtctg gaaggagttt acaagttcat tgttcaaata 161820 gtatttgttg ggggccaact acaactacac atcagggatt gtgctcacat tggactagtg 161880 tctttggaaa acagagtcct tgtgtgccct ttttgaattt acaggccaac ttggggaagc 161940 agagagacaa ataggaaatc caacagcatg ataagtagta taatgagaat gtttagggag 162000 atacgagcat ttataggagt agcactcaat aggaaatcaa ggaaggcttc ctggtggcta 162060 tgatgtctca agggagactt gaagcatgag taggagtatg tcagatgaaa agggatggga 162120 ggaagatttc aggtagggta atttgcatac gtgaatattt agacccagag aaagtccaga 162180 gggactgagg aacttaaaag aaatccagta tgggaggagc atagtacctg ttggggaggg 162240 tggggactct tgataaatga ggatagtaat tcaagttctt tggctcttga gttacttttg 162300 gacaagttga gcttcaagta gctagtacat aggaggagct taataaatat ttattgaatg 162360 aatgaacatg taagtaaaaa tgtctaatag gtacctggca aagtggactt cctagtcagg 162420 aaagagaggt tggttggaga tttctatttt gaaattttca atgtatatat gaaagtaaaa 162480 acacaaggag tggttgtgat catccaggag aaggtatgca gaggagggaa gagggtctag 162540 gggctgaatc ctggggtacc cacacctgag aggagggaga ggaagagggg ctctcagaag 162600 agcctgagca ggaagggcca ttgtaatagg actaaacact aagcccctga agtcagctct 162660 ctgggggcag aggtttttgt tctgtttatt ttgggatgcc tgctgttgac cagattatgc 162720 tcccctaccc acccaaattc acatattgaa gccctaacac tcaatgtgac tgtatctgga 162780 gatagggtct ttgggaggta attatggtta aatgatgttt.taaggctggg accctactct 162840 gataggactg tggccttata aaaagagctc tctctttttc tgtcctcctc cttctacctc 162900 ctccaggacc caccccaacc ccacactgag aaaataaatc gctgttgttt aaatcaccca 162960 gtctatggta ttttgttaca gtagcctgag ctgaccaaaa cagttctccg cacttagaaa 163020 tggtcctggt aaatacagct aatcaatatt tttttgagtg aattgaatga atgagtcatt 163080 cactggcatg cttataaatg cattactccc tccacagtgt gattgtcatt gagcaggttg 163140 ggagaaggga ggagctctcg caaacatgga agtcctgggt gcttagggag aatgggaagg 163200 tgccgtggtc acaaaatcgg aagaaagagc tgaaaggctg aaaggctgaa aatgctaaac 163260 tgctcaaatc tcctctgtct ttttttaaaa aaaatcattt ggctaggctg gctctgaaat 163320 tggggatggt tcttggttga ttatgtggtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 163380 taaaggccct tcttaacatt agataggact atctgaatct aagtgaattt aggcacaatc 163440 tagatattgc tcttcataga gaaataatac tctaagctgg caatgaaatt cctcaagtaa 163500 acaacagata aagggaagga gagggacaca gtgtaacaat aattggaata attggaattc 163560 ttgtatcact catggcactt acagtgaaag tactttctac attgtcttct attacatgca 163620 acataggtac attatataaa aagatcctaa aatattgtta aaagaaattt tcccagaaaa 163680 tgtaagataa aagttactaa attctattaa gcttgcaagt taaatccaaa tcattcattg 163740 actcataatc aagaacagtc atgctgcctg acaaccaaaa aattcaaact gtcttcatca 163800 agtcctgggt gggaagccag gaaatgggat ctgagatcac aggctttgtg agagtcaggg 163860 tctttggcgt gattcttggt cacaaatcat gcattttttt ccttggagag atagtgggaa 163920 agaaaaggct gtttaaaaac atgttaaaga atactgattc catttgactt cttgttaatg 163980 tgtacccttg cagacttggg taaaacttga tcatgttaaa agccattgta cttaaagatt 164040 catgactaga gtgttaggac aattctgtgg ccttgggggt cccattgcag caagaagagt 164100 Ctgactgaga agaggatagc tccgtccttt gatgcctagt aacctgcatg cccagcactt 164160 tgctggctgg gccattggta ggaaatgcgg tacatgcact tcaatctgta agagatcatg 164220 gtccatagag atcatagatt tattttgcca cctggcccag atggtgatag aagtcaggac 164280 aatggttgcc tagaggggtt gggagtttat tagaatggga cacaaaggga aatttctggg 164340 gtgatggaaa tactctatac tttcattggg atgattgtta catggacata tccatctgtc 164400 aaattcatca aatggtatgc tttagataca tgtatttcat tgtatataaa ttttgcctca 164460 agaaacaaaa actcaaggtc aggtgaaaaa agtccaggag ctaatcaaaa acccttaaaa 164520 taagtgacca aactatttgt gaaaatgagc agaaacttaa agggaaattc tttgttattt 164580 ttccacatta gaggaaaatt gatggtatca gataaatgct caaaaataag cttccaaaca 164640 gagcatttat aatcagtaca ataataagtt tgaacacaga caaaccttca tgtctcagag 164700 tgaaggaacc atttctagta gtataagcta ttacggagat agttccacat ttattttctt 164760 tcatgtttct tttggaggaa taatgttttt gtaaatgtaa aatgctttta atggagctag 164820 agaagaagct atgtttggtc atagtattat ctgcttgcga actaaaaatt cagagctctc 164880 ttgtgaatca tgctgccaag aattgcagac ttaagcctca agattgtacg aatttaaatt 164940 ttgactttat ggcctcagtt ggcatgaaga tacaaagata atcttctagc aaaaacagtt 165000 ttgaaagacc gggcgtgatg gctcatgcct ataatcccag cactttggga ggttgaggca 165060 ggtggatcac ctgtggtcag gagttcagga ccagcctggc caacatggtg aaatcctgtc 165120 tcaactaaac atacaaaaaa attagctggg tgtggtggcg gacacctgta atcccagcta 165180 cttagggagg ctgaggcagg agaatcgctt gaacccagga ggcagaggtt gcagtgagcc 165240 gagatcacac cagtgtactc cagcctgggt gacaaaagtg aaactctgtc tcaaaaaaaa 165300 aaaaaaatgg tagaatgtca catggaaagt taacttttag agatagtaat ccagacacct 165360 ataatattac attgtccttt taagtatcta tatcaataaa ttaaccaaca tttgttgact 165420 taacatggcc agggacagtg ctgggttctc ataattccca agtaattcta gccagacctt 165480 gtcctcaagg agcttatgag cagcatgagg agataaaaaa cagataaata actctaattc 165540 aggaagaatt tgataaatgc atgataacta ctactagaat tcagaggaga ggaaattttc 165600 tttgaataga gactaaagga aggaataatg aatgtggtag catttgggct ggacctatta 165660 caagaggcat gctttcccct gacaagccaa gaaagtgggg atttaaggta gaagaaaaga 165720 aaagccttga gttcttggag tattaaaatt ttgcttggct gaagcatagg ttacagtcgg~165780 aggagatgga ctaggaagga tcgttatggg cagagaggaa gccctgaact catgggggaa 165840 gctatgattt gggttaaaga cagagctggg tcatgtcaag atggatcctg aagcagtaaa 165900 aaaaaatcca agaaagtaaa caggttgcag ggtttaggat gaagtccggg aggaaagggc 165960 agagtggtcc ttaggaggcc gttaggacag gtaaggtaat gggtctcaaa gggagtggcc 166020 gaaatgcaat ggaaaaagag agattgtaaa gctagaaggc ttaggaattg cctcttgatt 166080 aggtgtggaa ggcaagggaa aatcagccct cgaagaagac agtgagattt taatctgggt 166140 ggctggagag acagtgatgc tggcacagac acgggaagtt gagaggaaca ccatgtttga 166200 gaatggtgac tcatatttga acaagcctgc aatgcccagc agaccgctgg aaaagtgggg 166260 ctggagacac attcaacgga ggagccagat caatctttac ccttcttcac ctgagagagc 166320 cagtaagtca cggctggaac gtgtgtgtcc agcaggagag ggtagggagg gaagccaaga 166380 gagctgggag cccaagagtg aagtttttgc caaaggcaga agaggaaagt cggcgtagca 166440 cagtatactt tcccacccat gctcaccaag cccagggaca aggctcacca agatgagttt 166500 ggaagagaat gctggagaga aagtggttaa gaaaactgcc tttactgaac ttcttgggct 166560 aactttgatt gtaagtctct gaacaatcaa agcctgtgag gagacagcca accttcttat 166620 tcttcctatg tcaatagtga acaattgcag atcccctttc ctttccttct cctttcccct 166680 gttcctctct cctccctccc tgaatactct tgcttttttc tgggactggt ctagagcatg 166740 ggtggccatt gttgacctac aggaggcacc actgtcacca acaaagggta acagtctttc 166800 ttttcaatat ttatttatat ccagtattta ttttcaatac tgactatgga gagagctctc 166860 ctgtgctcaa acactgcaat actgggggtc tttcaaagca caaaaacata tatttgcatg 166920 atggcatcat taacattttt atggctttct atttcttttt tgtactggtc tcaagagcca 166980 ctcataaatc tctcagtaac tgcatagtgt cccagggcca gagaccggcc actcctggca 167040 ttgtgattag agtcatttaa tatccaaggt ggtgactaat gtctggcaac aaagcctcca.167100 ttgggtgtca tgtgtcctgg gaccctgagc gtgggcactc taggagcacc tcagtattgc 167160 gtgttagtac tatggccgag agaatagttg agaaagtggt caagaggtgg atccatgtga 167220 acgccactgg gaaatgagag acctcgttcc caatcacggt cagtgcaact cgaaagccta 167280 aaatcagttt aaaacaaagg tatctacctt tatcttatgt tcatatccta ggcttttaat 167340 aatacgtatt tttcacatgt ttacagaaag cagtcaactg agctattcat ggaaaggttt 167400 gtgggtttgg ttaacgaagt ggaggagtat tacatttcag ctggaaacac atccctagaa 167460 tgccaaaaca tttattccaa agtctggttt cctggtgcaa tcggaggcat ggcaatgcct 167520 ctgttcagag actgggggct agggccagta aggcatttga tccacatgta tcccagaagg 167580 cttttattgt taaattatat tctttcggaa aaaccaccca tgtcctattt tgtaaacttg 167640 atatccatac acttttgact ggcattctat tttagccgta agactatgat tcacagcaag 167700 cctgtttttc ctcttgcttg gggtggcagc agaaagcata gggtactttc cagcctccaa 167760 gggtaggggc aaaggggctg gggtttctcc tccccagtac agctttctct ggctgtgcca 167820 cactgctccc tgtgagcaga cagcaagtct cccctcactc cccactgcca ttcatccagc 167880 gctgtgcagt agcccagctg cgtgtctgcc gggaggggct gccaagtgcc ctgcctactg 167940 gctgcttccc gaatccctgc cattccacgc acaaacacat ccacacactc tctctgccta 168000 gttcacacac tgagccactc gcacatgcga gcacattcct tccttccttc tcactctctc 168060 ggcccttgac ttctacaagc ccatggaaca tttctggaaa gacgttcttg atccagcagg 168120 gtaggcttgt tttgatttct ctctctgtag ctttagcatt ttgagaaagc aacttacctt 168180 tctggctagt gtctgtatcc tagcagggag atgaggattg ctgttctcca tgggggtatg 168240 tgtgtgtctc ctttttcttt caggacttgt aggattcttt gtgccatttg catataattt 168300 ggcaggttca cattttttaa gagccctatg aagtgctttt tgcatgtgtt ttaaaaaggc 168360 atttgaaaat tgaaagtgtg atttatggaa attaaatcat ctgtaaaaaa ttgctttgga 168420 aagtaatgat tgctggccat aaagggaaat atctgcgatg cacctaatgt gtttttaacc 168480 ctttatttgc tgacaatcta tagtcattaa tgctaaactc gattttggct tcagctacat 168540 ttgcatattg tccaacaatg gtctattttt gtaagaatta gataaaatgt atacttgata 168600 taaaatagtc aaaaatgtaa ctcttagtaa cagtaagctt ggcatttaga tagaccatga 168660 acacttcgtc agatactctg ttgggtgttt gggatagcaa ttaaaacaaa gtattgatag 168720 ttgtatcaga gtctattagg ctgcagcaaa ggaagtttat tcaaaagtat aaactatcca 168780 agattataga cgcatgatat acttcaccta ttttttgtct ccttaatatg tatatatata 168840 tatatatata tatatatata tatatatata tatacacata tatgtgtgtg tgtatgtgcg 168900 tgtgcatgtt taacttttaa ttcagttaaa aacttttttc tatttgtttt .tcatctggat 168960 atttgattct gcatatccta gcccaagtga accgagaaga tcgagttgta ggactaaagg 169020 atagacatgc agaaatgcat tttaaaaatc tgttagctgg accagaccga caatgtaaca 169080 taattgccaa agctttggtt cgtgacctga ggttatgttt ggtatgaaaa ggtcacattt 169140 tatattcagt tttctgaagt tttggttgca taaccaacct gtggaaggca tgaacaccca 169200 tgtgcgccct aaccaaaggt ttttctgaat catccttcac atgagaattc ctaatgggac 169260 caagtacagt actgtggtcc aacataaaca cacaagtcag gctgagagaa tctcagaagg 169320 ttgtggaagg gtctatctac tttgggagca ttttgcagag gaagaaactg aggtcctggc 169380 aggttgcatt ctcctgatgg caaaatgcag ctcttcctat atgtataccc tgaatctccg 169440 cccccttccc ctcagatgcc ccctgtcagt tcccccagct gctaaatata gctgtctgtg 169500 gctggctgcg tatgcaaccg cacaccccat tctatctgcc ctatctcggt tacagtgtag 169560 tcctccccag ggtcatccta tgtacacact acgtatttct agccaacgag gagggggaat 169620 caaacagaaa gagagacaaa cagagatata tcggagtctg gcacggggca cataaggcag 169680 cacattagag aaagccggcc cctggatccg tctttcgcgt ttattttaag cccagtcttc 169740 cctgggccac ctttagcaga tcctcgtgcg cccccgcccc ctggccgtga aactcagcct 169800 ctatccagca gcgacgacaa gtaaagtaaa gttcagggaa gctgctcttt gggatcgctc 169860 caaatcgagt tgtgcctgga gtgatgttta agccaatgtc agggcaaggc aacagtccct 169920 ggccgtcctc cagcaccttt gtaatgcata tgagctcggg agaccagtac ttaaagttgg 169980 aggcccggga gcccaggagc tggcggaggg cgttcgtcct gggagctgca cttgctccgt 170040 cgggtcgccg gcttcaccgg accgcaggct cccggggcag ggccggggcc agagctcgcg 170100 tgtcggcggg acatgcgctg cgtcgcctct aacctcgggc tgtgctcttt ttccaggtgg 170160 cccgccggtt tctgagcctt ctgccctgcg gggacacggt ctgcaccctg cccgcggcca 170220 cggaccatga ccatgaccct ccacaccaaa gcatccggga tggccctact gcatcagatc 170280 caagggaacg agctggagcc cctgaaccgt ccgcagctca agatccccct ggagcggccc 170340 ctgggcgagg tgtacctgga cagcagcaag cccgccgtgt acaactaccc cgagggcgcc 170400 gcctacgagt tcaacgccgc ggccgccgcc aacgcgcagg tctacggtca gaccggcctc 170460 ccctacggcc ccgggtctga ggctgcggcg ttcggctcca acggcctggg gggtttcccc 170520 ccactcaaca gcgtgtctcc gagcccgctg atgctactgc acccgccgcc gcagctgtcg 170580 cctttcctgc agccccacgg ccagcaggtg ccctactacc tggagaacga gcccagcggc 170640 tacacggtgc gcgaggccgg cccgccggca ttctacaggt acccgcgccc gcgccgcccg 170700 tcggggtggc cgccgcgccc ggcaggaggg agggagggag ggagggagaa gggagagcct 170760 agggagctgc gggagccgcg ggacgcgcga cccgagggtg cgcgcaggga gcccggggcg 170820 cgcggcccag cccgggggtt ctgcgtgcag cccgcgctgc gttcagagtc aagttctctc 170880 gccgggcagc tgaaaaaaac gtactctcca cccacttacc gtccgtgcga gaggcagacc 170940 cgaaagcccg ggcttcctaa caaaacacac gttggaaaac cagacaaagc 'agcagttatt 171000 tgtgggggaa aacacctcca ggcaaataaa cacggggcgc tttgagtcac ttgggaaggt 171060 ctcgctcttg gcatttaaag ttgggggtgt ttggagttag cagagctcag cagagtttta 171120 tttatccttt taatgttttt gtttaatgtg ctccccaaat ttcctttcat ctagactatt 171180 tgattggaaa tatgtcagct atgatgatga ctttctggga agcgattcct gtcacccgct 171240 ttcccctcct ccccacccca cgtcctgggg ctttagagag cgattgggag ttgaatgggt 171300 ctgatttcgg agttagctgg ctgagtccgc gctggagcgg attgctggca tgtgacttct 171360 gacagccgga aatttgtagg tgtcccgcga gtttaaaaca agccatatgg aagcacaagt 171420 gcttaaaaat aatctcctgc cagcccagtg acaagcctgt cccacccggg gagaatgccc 171480 cggagtggcg tgcgggtcag ccagggtctg cgcctcgcag ccactgtgga aggagcgcgg 171540 ccggtccagg acacaggaga ccactttgtg acttcaatgg cgaaggttgt gtgtcctcat 171600 tttaattttt ttccctacaa gaattgttct ttctccctct cctctccctc ccattttctc 171660 ttgcccagtt~tctccttttg ttttttgttt tttgttttcc tgatgggcct gcagagggat 171720 taggtgggcg cttctggtga acaccttcct aggtggccac aggacaggtg taccccggac 171780 tgggtttgga agcttcaggg cgccacatgg ctgggtcctg aattaggcat ttcccaactg 171840 tacactggta tccggactgg tgtccctata tctttctgcc ttgtaagccg tggaccagtt 171900 tttgttcagt attct,gtttc cagggatatt tatagcagaa ggaaggggac taaagtgcag 171960 tttggcccca gaggatactg aagggcagat tctgggggta ttcagtgtgc atcttcagcc 172020 gccttggaga aatttagagc atcccacagc cacgcagatc caagctgtct ttactcaaaa 172080 gacaaacaat gaacaagact tttaaaggtt ggcatatttc aaattaattt tacttgtttt 172140 aatttagggt taaaacagag aaaaaggatt tcttctgccc accttttttt ttttaaatgg 172200 aagaacaaag tacagcgatt aagtctaatt ccacacaaca tttaaaactg cttgatgtga 172260 aggaaggcac tggtatgatg tgaattccat aaccttatga tggactccag aaaccatttt 172320 cttccctatt taattttcag ttcttttatt gcaaattaat gctgctgaat ttcaatgggc 172380 actaatgaga ctgctccttg gtagattatt tactgccttg ctaataatta caaagtgaac 172440 ctggtcaaat acagagggga tcgcatctta ttcaaaattg ttcatcatcc cagtgataag 172500 tggtatcagt gtaatatgcc ctatcttaca ctttctgcat tacatgatat tcaaacactc 172560 ttagaataat aaaaaaagag acaaggaact taaaaattaa aaaaaaaact tgcacaaatg 172620 ggactctgtg tggaaattca gttttagaat gatttttcct gtgttttatt tcccggatta 172680 tctttcctct tttgttagaa ttctgcctgt tattatccag caaggaaaag aagcatctat 172740 gcaagttctt catatggaca gatattattt agtatttttc ccctctcagt ttttctgctt 172800 aaatgactct gggtataaag gaaaggattg attgggctct tttaggaaac tttaagtttc 172860 ttaagtagtt ctcaaaagtt ttggggctga aagcagtgtt ttcaaactgc ttgtcatgac 172920 ccagagggtc atgaactcag tttagtgagt ctagaatatt ttttaaaagg actaaaatgg 172980 aaaggaatat aatagaaaat atcagagtgc atggtatttc gtaaggataa gttttgtttc 173040 ctgaaaatct gttttaatta tatgtgcttc tgtgtgctga ttgtgatgta aaatgtattt 173100 cttactgtgg attgaattca aagaaaaaat tagaaagcta atggcctaaa atattatatg 173160 ttcagtagaa aacaaaaaat tcaggcaagt ggctggttgt ttttacctat acaaatcaaa 173220 aggctatttt gattgtcttc attttcccct tataaattag gttggtgtct ttagtcattt 173280 aggctaagtt ttactatctg attcttaact tttctattgt agaatggtgc tgtcatgtgg 173340 actgtcctcc cgaatgtccc actggatgtt cagagaattt atgtgaaggt cacgtcattt 173400 agcattgaga tgctgtggtt accttcttcc atttcttcca taatatgcag ccacatctat 173460 gtgtgaagaa atgtaataga taaaatttct ctggacgcat aataatgtga gaaagattgt 173520 cacatgtccc agcaaattgt tattaatata aatttgttac ttggcaagct gagattttgc 173580 aagatgttac tcaaaatttc acaatgaagg aaacagggag tcatcttatc ctgggttcct 173640 tttttagatt tcaaacaact taggaacttt gaataaaact aaagatgaag cttaactata 173700 tcaactatcc ttttaaaagt tctaattagg aatttaatgc tgcatgctta tttcagtttt 173760 attactcagt attcttaaaa gttagacgtc tctcacttct ccaaaaaact tggcaaatgt 173820 ataaatcttt tgcatcaaaa tcaatgccct gctaatttgt atcctggcca tctgcatatt 173880 ttggacaact aatttttcca ctggtgatca tttgaaactc tttctcaact ttgaatagag 173940 actgatttcc aaagtgagat ttaagtgact aagtttcaag tttccgatac atttttcctt 174000 ttacttagat aacatttcag cccccttcct ttctgatctt acttttttat taatttaaat 174060 tgttactgat tacgtgacac tttgtgctgg tctaagaata gtccagagtc acatattccc 174120 tggtgaatga gcatattttc ggatgaaaac ggaatcacat cttcaatccc catttcattt 174180 tcacctcctc catgtggctt gtacctgttt ggaagaaagc tcctgaagga taattgccac 174240 ttattctaat ctttctcaca ctcatttaat ttggatccct ggctaaagtt gttatttact 174300 tttgtgatta tacttagtct atgacattca taatttggga aaattctcag gtttgagaat 174360 tttggcggct tgggatttct tttagtttct tatagtttta aggatatgta agacaggtgt 174420 aagaaactgc caaggggagg aaccatagat atcaggaaaa actagaaaag atgccagact 174480 taccattaat gaatgatgag acaatagtaa ctttgttaag tgagattgta tatgtgaaag 174540 tggtatagaa actaaacaaa cattaggtgt ttttattatt ttactcacat gttaatattt 174600 gttttggtgc tttcataggc taaaaagctg ggaaataaca gatttaagtg gtcaggaatt 174660 ttgttataaa tatagaatga tgattatatg aaatcttttc ctgtgaaagt caaatttaag 174720 taaaatcttt atcaccatct gcaacatttg tctgcagcct ggcttaccag gttatcataa 174780 agaacattta ttttacagat acattaaaga aagtcaaaac cctgattatg tgtaaacaat 174840 tttacataag gaaatatatg aattttaatt atatttttct aaaatccgta ctcagcatga 174900 aattaataca tcttaacccc tccctgtgac ttcattatta tttttaatgt aactttagaa 174960 gaacccagta gagagagcag cgtgctaagt gtgtttcttt cttttccaga caactttgaa 175020 tggagaggag caaattagtc ttttggttta attctgtctc agtttgctta tctaaagaaa 175080 ggaaaacaga gtggctacac ttgtttagaa ccatatgcat actccagaga aagatgctct 175140 attaatccaa aaaatacagc cacttgaaac cagccaaagc gaaagtgtaa gggacttcat 175200 ggaaaggagg cagttcacca aagttattga ggggttttat attttaaact ccgccagtga 175260 attgacgtgt aatgtcactt acaaaaaaaa aaaaaagtat gtctgagctg ttcgctactt 175320 cgtctctaaa atatactcat actgatctct gaaatcccag aatttaagtg ggctggaggt 175380 tacgggaagc acctttataa tatccttaat ctcatgaggg aagaaaccat aattgctgaa 175440 ttctctgcct tggataatat caggagggac tctgaagaaa gttttgcagt aatcaacaat 175500 gttttaaatt atgtgtatat ttttagatca cctcaaaaaa tataggaagc acagaatgac 175560 aactattctg gtctcaactg acacaatttt atgtagttta ataaagtaat aatttcaaga 175620 aacgtgggca aataagaaag agtatgactt tcttacaacc cgcttgtaag tgatgtggtg 175680 gtggtaatga tccatgattt tgatgatgac gatgatgatg aaaatgaagt ttttgtctca 175740 gtttgggtag gtggtatttc tggatgcctc ctatggaccc tggagatgtt catcctatac 175800 agaaatccaa tcctttaaat ctacttggct cattgtttta gaattctaat tccatagtct 175860 gaaaatttta ataatgatat taccaataat attagaaact tattaagtac ctataattgc 175920 tatacaaaaa atttaaaaga acccaaaatt ccaagcaaga ctgaaaattt tttgtctctc 175980 ctctgaacta tttagaggga caaattagtt tgttcttata atatctactt taaataaatg 176040 tgccatcttt aataagatag tagacttctt tgtttggtaa tgttctattt tttggagatc 176100 ctatgagtta cacttgggaa aattataaaa gttcacttaa agttaataaa atccattaag 176160 taatgttcag aactagacat ttccaaatga gcccttgaaa agctcaggtg ggttcttttt 176220 gagagttccc caaatgttgt caaccccagg aggaatggaa gacctctgca gttttgttat 176280 tcagattctc atctccttct cagaagccgt agaactggcc gggccctaag gtccacgctc 176340 cttggttcca gttCtgtCtt CCatCCttCg gtcccgggct Cattctgcct gttcctaaac 176400 ggtggcaagt taggggcccc agcagccaac ttgtgcttac ctggcactac ttcctgggca 176460 gttttcttgg ctccttgact tgttgggcgg cttgggattt cttttatggc cctgaaagca 176520 aaagacaatg ttctctttta gtttcctgca attaaatgat gttagaaata gtcatcttca 176580 cattggcgta cttcctcttt cttctgtagg tcttttagaa ttttgagtcc attctcatat 176640 tttcttgttt catttgcttt attttctaat acatagaagt ttaaactccc tttaaagagt 176700 ttttggcctc ttttacccta ttaagctttc ttttttcttt tctgttttag ttgttccatc 176760 tgtgtattct cagatatttt tctttcacct tttctggttt atttctttat tgacctgtct 176820 catctgttat tttaatgaaa tttggaacag ggctaaacag agttcctacc tcagccagta 176880 taagaatata ccgtaataac tcagagtggt attaactaga ttaaaagttt caaaaagtga 176940 tgtttttctt gtctctgagg atagaaactt caacaaaata aagaagaaat tttcaattag 177000 tagaatttct ttgaaagttt gttcattcat tcatttggct accttattcc aaattgagtc 177060 attcattgag ggcttagact atataaagtg tggttttgtt ttcccagcag ttcatgcaac 177120 agcattgcac ctagcagctg ggaagtctta tagcatgaat aggtgagatt ctaataccag 177180 aatctcctgc atgtgtaaac taacagtgta gtcttgactg ttgtctccca gtaaacttgg 177240 tttcaggagt tttagatcca tgtgaacgtg tacaaggcat ttttgctaac tgtaacttcc 177300 cacttaatca acaaaaacaa aaacactcat ttctgaacat tcagtgcatt catgattaat 177360 cttaattaca ccacaaaggt atttttcaat ggtgattttg cgggagtggg gtaacagttt 177420 cgaaagcaac attgtcagaa acatagttga ttttaaaggt tctttctggt gactttgact 177480 tctgcttttt tagaagacct tacacagagt tgtatttatt tctcctggaa tatttcaagc 177540 aattcagagt gaaagggtat acattccaat ttgcgtatga gataaaattt agttacattg 177600 agaagctatt ttctttagtt acagggaaaa aattgtaggg cttttggaag cctctttgat 177660 ttctaatagg aggaatccct gagcactggt ccaaacagaa atcatctctt cttcattgct 177720 gtatttccct caagctctta gcaaagtgca tggcacgtga aagcccggag aagctgttgg 177780 ttgaaagaat ggatggtggt gggcaggaag catcagggac atggtttgct tcagtctatt 177840 ggctgggaga aaggccattt aggaagggat ccttagatgc cactggaaga atgtgggaag 177900 tttgtgaatc tctctttctc aggaacaaaa gtagaaaaag gactccacac agcattccaa 177960 gtacagtcgg ccctcattat tcatggattc tgtatttgca aattcgctga cttactgacg 178020 tttatttgta accttcgagt caacactcac ggtgctttct cagtcctttg cagacgtgtg 178080 gaatggcaaa aaaatttgag ttatatgacg tatatgttcc cagctgaggc tgagcaaggc 178140 tcacttctcc ttgcagccct cagactataa acaagtgtcc ctcttgctat ctacttcgtg 178200 ttatgatttt tgcattttca taatccctgt tgatgatttt gctgtttaaa atggccccta 178260 agcatggtcc tgaagtactg tctagggatt ctaagacaag gctctgacgt gtcttaagag 178320 aaaatacgtg tttgataagc tttattcagg catgagttac aatgctgttg gccatgagtt 178380 caatgatggt gaatcaacag gatatattaa atacagtgtt tttgaacaga aaaacatata 178440 aaacaaggtt atgtattaat gagttggcaa aaatgctgtg accaaaggct cccaggaacc 178500 taccctattt tcccctcaat gcaatggttc agtatttgct aattcagtgt ttgaggtgac 178560 tttatagaac atgagtacca tgaataatga gaatcgattc tgtataatag agtgatgaaa 178620 gcacaggtct gggagccagc agctatattt ctattctggc gtgactcctg tgtagttgtc 178680 atcactggca aattgcttaa ctgtgtgcct cagtttccta atctgtaaaa gctacatcgt 178740 ttggatgatg tgaggattaa acaaattcat agatgtctag ggcttataac attcctggca 178800 cataacaagt cattattttt tattactact tcggaaggga attgagtact ataccctgaa 178860 gaaggtgagt atgggaattc tctacgggtc tggaatgtcc ctatatttgt ttattttgcc 178920 ttcaagtgac taactttaat accctattgt gattagaagt taaacttctg caaccaaaag 178980 gaagcaggaa gctagtattt cttgaagtgc ttattacatg ccaggtactg tgctacaaaa 179040 acaaaacaaa acaactgtaa aaaaaacttc aaatttggct gcgtgcagct gctcatgcct 179100 gtcatcccag cactttgagg aactgaaggg aggattgctt gagtccagga gttccagacc 179160 agcctgggca acacagtgag accctgtctc tacaaaaaaa caaaaacaaa aacaaaggca 179220 ctccaaatca gtaaaaatta atcaatcaat aaaaagagtg aggggcatta agtattgtgg 179280 actgaagcaa tcccagagag ggaattaatt gaagctgagg taagcagctt atggagaagc 179340 tatgatgtac agagggcaag gaaggaattt ttctgtaatt tggaaaaatg ggaactgtga 179400 gaaagaagga gttggaagct catacttagg gagcatctac aaggacgtct ttttcacgtt 179460 ggttggaata tccaaatcaa ggattatttc agaatcaccc agatgattaa aaaactactg 179520 agatccaggt tgtatttcag cagttctgac aattgctctg ggtcgaagct tgaatcagta 179580 gttaagaaaa acaacaaaca aaacaaattt tggggctttt ctcactgatt tacagttaaa 179640 gctcatttac tcttcctatg actttagatg gaggatattt ccaagtcttc aggatggaga 179700 catggaggga agtgagacta gtgatgtgcc tcaaggtttt gctgttgttc taaccatgag 179760 gagcactatt caaacccagg tctgctagat ttccaagtct tcatttcctt gggcctcttg 179820 gatttcagaa gcagagggta aaaggagtgc tggggagaaa gatcacagta gctttcaatt 179880 ctactcctca gctttccaaa ataagtttca agactggccg ttgcatttga tatggaataa 179940 atacaaagaa ggtagattga agggtatgaa gatgcagatt tttgatacca gatatgaaga 180000 taacattagg aagcaatcta aaacatggac acaaacacac acctgtgcca gttagcctgt 180060 ataattcgat ttttgttaag tgtttagata actgaaggta atttaagccc tcatatcttc 180120 ccttcatagg gttctttttc cctctggttc atcagagagt tgccaccaat tcaggctgtt 180180 agtggtacac ataacctcta gcattgttga tacagctata aaatcccaaa tatcagtaca 180240 attgttgatt gcataaaatt tccagttgca tggttggaaa gtcctgtaag tttgaatcct 180300 taaaccagtc ttaaatgtgg aggaggactc aattaaagct ctcctcgtgt cctccctctg 180360 acgtatttgc aaaatccttt ccacaaatag aatactgttt ttaatgcttc cccagtccaa 180420 ttttgcgttg tagaagacga atttatggat gagggaagtg gcattcaggc actccagctt 180480 ggtatagaag cccatggtgt ctggtcctca gtcctcaagc ccgctcattt cctcatgtga 180540 actcagaata agcagctgaa agcaagtctt caaaatctca gagatatgta taaatgcaag 180600 tgtttgggtg agaagtgaac atgggtctcc tctagtgccc acactacttg actaacaggt 180660 tttgggctcc acacaatgag ggattatcaa cccctgtccc agggctctct gggtcttggt 180720 tctttgtttt tgatgctcag caattgtgat cagtgaaacc aatgttgctt ttctatcaag 180780 agtccaaccc ttttctaaga agggttgtgt ttgatattag ggaatagcta gcaaagttat 180840 caagtaactt gtagaaacat tcttttgcaa gagttcttat actgaatgac tgtagttgac 180900 agcagtgcag tactggtcat tttctaggac atcttaaaaa cactgatgag aagtttcctc 180960 tcagatgtct gtcatgtcat tcttgccttt ctctacacag ggtcagtttt ctcttattgc 181020 tgttaggagt tcctcattgg tttttcagct tttgggcttt caaactctaa ttaatcataa 181080 SO

gctactagag tgtactacct aaagtgtgta tatacacata tatacacaca cacacacaca 181140 tatatatacc tggtatacat atatatatat aatatacata tatcatatat actccccaac 181200 ctgatctggt tcttcctctg cataaaagac ctcaggccag tcagagaaaa catgtatgtt 181260 ccatggtgtg gcaatcaagc ccttgtattt ggttccaatc agtctcctaa ctattactcc 181320 aagaagctct tgttgaaaga gccatgttta aatggcatgt tcctactttc ttcttcatag 181380 tgatcttcat ctgtaccatg tacccttctt tcttcttgtt ccatctctgt taggctgatt 181440 ctacccagaa gtcaaggttc agctcaaatg ctatccctat caggtgaatt ttccacctgg 181500 catttgttcg gtgtgcattg tgtgcataca gcaccttttc ccggtacctt tactgtaatc 181560 accagataat tcctttcatt ttagttgtaa atagagttgt cttccccctc tatggaatag 181620 attttattaa tgtatagagc agcagtcccc agcctctgga ccatggactc gtactggttt 181680 ggggcctgtt aggaactggg ccgcacagca ggaggtgagc agtgggcatg caagtgatgc 181740 ttcatctgta tttacagctg ctccccatcg cttgcattat gcctgagctc cgcctcctgt 181800 cagatcagcg gtagcattag attttcatag gaatgcaaac cctactgtga actgtgtatg 181860 tgagggatct gggttcttct tatgagaatc taattcctga tgatctgtca ttgtgtccca 181920 tcacccccag atgggactgt ctagttgcag gaaaacaagt tcagggctct cactgaatct 181980 acattatggt gagttgcata attatttcat tatatgttac agtataatac taatagaaat 182040 aaagtgcgca ataaatgtga tgcactggaa tcatcccaaa accatcccca gttccatctg 182100 tggaaaaatt gtcttccatg aaaccggtca tggaactggt gccaaaaatg ttggggacca 182160 ctcttataag gcatattaga gtaatttcat agatttccta attcatttat catattcatt 182220 cactcagcaa gcattactgg atgttgatca tgtactggct ttggtggtag gtgcagagat 182280 tgggaacatt gtcatcaagg agtttatggt tgagtgaggg agatgacaag tggatagaca 182340 atgaaaaaac agtagaataa gaactgtgat'agaaaagaga cagccaggag cattgaggag 182400 aggcacttaa ccagatggag gatcttggtc cattgatatg gaggtcaaaa tggtttaata 182460'-gagcaagtga ccctttcaac tgaatttttt aagaatgagg atttagccag acaaagaagg 182520 gcaggtgagg ttgtgaagag gaactgagtg gtactcttca gagctccagc ccagttcctt 182580 ggacagaata aatgcttact aacttataga gctgaatatt gaattaataa aataagggta 182640 aactgttaag aatcagagaa ataacttaaa gaacactgat agctagtgtt ttttgaacac 182700 catgtaccca ggtgccttgc cgaaaacctt aatgatcatc ttgtttaaac cttacatttc 182760 tcataagagg ctggtactat tgttattctc attttatggg acgtagaaac taagacttgg 182820 agaggggaag tgacttgccc aaggtcatac aaccagtact ggagaattag ggattctaga 182880 tctagaattt ggactctgga gcttaaggtt ttaacccacg acattatgca gagaaattga 182940 caggattttt ctgttgctga tcaatttact tggcagttag tttgttactt ccttgtcttt 183000 attttagttg tgacaatgct ttcatcttag actgtgtccc gaggctgctg cttttatttt 183060 tatgggaaat ggctattttt atgatccttg ctaaaagcat gtttaaacaa ttttccatta 183120 agtaggggga tgtttttcct tctaatatca gaagccaata aatgaaattc tacaaagact 183180 tgctggtagc aaccttagga atttctttgc atgtgaaacc catctgagaa cttaaaatct 183240 gggtaaaatt gtagtgtaat ttggtgcaat cgtctctttg cacaaataac atcataaaat 183300 catagtattg tcatctagga ggggccttag acatgatgga atcctacctt ttatattttc 183360 caggtgaaga aatca~agtc tagaaaggtg aaggaacttc ccccaaagtt tcccagctgg 183420 tagagacaga accagggcta ggtcctctat tctgactcct gaccactacc tcacacctaa 183480 tagatggagg catgcccagt tcctgttcac cgagggcatc agaccatgcc atactcattg 183540 ctactgttcc agcatttata gtagaagctc aagcaagcag gatgacagaa tacctaattc 183600 tggtcactac aacattataa tgatggctaa agtgaatgcc ccagccatgc ttgtctagac 183660 aggccatctg tttaattggt atatggttca cgtgagaatt tttaacctct gtttgtcgag 183720 tcggtgttag ttctctagtg atgaattatt tcctatactt ccatttagat tatttactct 183780 taatttaata accatacatt gtttactttg gtattgaaga ttcccttgtt tttcttcttt 183840 ttttctgttt ccagggctta aaggttagga gtgaccttgc cagacttccc tggagactta 183900 cactgtctcc tttcagattt ctgaagcagt tgggtgctat ttttagtcca ctatcaccaa 183960 tgtgaaaatg gaacttgcat tatttcatta tagatatttc actttagtat tgacagaatt 184020 aaaaaaataa tttgatctgt gcttgatcta gcagccaggt tacaatagac atttttagtt 184080 acctggtcca catgttgaaa aacatgtgtc ttctctgaga ctaatgacta agcccgatgt 184140 tggttatata ctgtttacta ttaaattttc cccttgtagt ttaatattgt tccaggaaat 184200 gaaatgaaag tttaataaga atggcaattg atggacccat atgtcggaag tataactaat 184260 gtccccgtta catgtgttaa agaaaggcat ggctggtggg ttgtaactgt actacaccaa 184320 gatgatttga cacaacttat tctacagaga tatatattta tcaggataga atttataact 184380 aaacaaaact atagcatttt ttcactttga ttttttttaa atgagtcaaa gaactgctag 184440 aattgtcagt taaaaaattt taaaaggaga tatgaaaaaa tcttacaatt cacaatgctg 184500 taaagagata atgtagggat taatatgttc ttgatatcaa tattttatga cttttataca 184560 tgtagaagca aaacaatttg aggtaggtga agttagtatg gacttcttga gattgtcctt 184620 cacatttctt ttcctttcgg tgaaaaattg aaggccaaaa tgtattttct tctggttttg 184680 aaaatactgt caagatcctt gcaacaaaat gagttcctct aaggagctga aaacaaagct 184740 cactcccctc gtgatactct gagaggcttt gctcagcatc ctgcattctg gtgattcctt 184800 ggagacagat gatgctaaac acaggaagat taggtcaatg gtaacttttt ctaagtcaat 184860 atttcttctc cttgggagat gatcatttta aatcttcccg aagtccaggc taaacctttc 184920 taattgaatc tccatgaagg agagctccag caggtggaga ggaagtgaga aagagaaatg 184980 aaagctgcac gcctcatgac gctgtgccag ggagttctta aaggtgaggg agtttctttt 185040 tggtaaccta agctatgtga atcagaaggt tcattagctt gtttcttttt cttttttgta 185100 aactcctaca taattttagt aaacaggaac agtaacctaa tgtgatatcc cactggccca 185160 agacttagtg catcttcaaa gttgcttaat tatgtccgaa acagactttt gtctcttgat 185220 gagaaaagca tggttaaacg tgtgatgatt tcctattgtc ctgagctcag atctgtaatt 185280 gtggccagat tcatgcatct ctgctgcctt ctcttagaag aatcatatgt aggcttgtca 185340 gataaaacag gatgcccagg taaactggaa tttcagttaa ataacaaata acattttagc 185400 atgtcccatg caatattata ctaaaatatt atttgttgtt tatctgaaat tcaaatttaa 185460 ttgaatgtcc tgtatttttg ttggttacat ctggcagccc tagccatgct gcctttctgc 185520 ttaatgggct taattttttg aaggctggag gttttctgtt atggtgcccg tttccacctg 185580 cttttctacc aggaaaggag gcatgctgat gtagaatttg catccttatt tttgtcatta 185640 ttattgatta taacagatga cataggttta gattaaacct acaatgacat tgctgtcatt 185700 cagataattg taattattgc taattgtaaa gaaggataat tttttttgaa atgactatta 185760 tttgtttttt gtttttgttt ttgtttttct ttttttctaa ttatacttta aattctaggg 185820 tacatgtgca caatgtgcag gtttgttaca tatgtataca tgtgccatgt tggtgtgctg 185880 cacctattaa ctcatccttt acattaggta tatctcctaa tgctatccct cccccctacc 185940 cccaccccac gacaggtccc ggagtgtgat gttccccacc ctgtgtccaa ctgttctcat 186000 tgttcaattc ccacctatga gtgagaacat gcggtgtttg gttttttgtc cttgggatag 186060 tttgctgaga atgatggttt ccagcttcat ccatgtccct acaaagaaca tgaactcatc 186120 cttttttatg gctgcatagt attccatggt gtatatgtgc cacattttct taatccagtc 186180 tatcattgat ggatgtttgg gttggttcca agtctttgtt attgtgtata gtgccacaat 186240 aaacatacat gtgcatgtgt ctttatagca gcatgattta taatcctttg ggtatatacc 186300 cagtaatggg atggctgggt caaatggtat ttctagttct agatccctga ggaatcgcca 186360 cactgacttc cacaatggtt gaactagttt acagtcccac caacagtgta aaagtgttcc 186420 tgtttctcca catcctctcc agcacctgtt gtttcctgac tttttaatga ttgccattct 186480 aactggtgtg agatgatatc tcattgtggt tttgatttgc atttctctga tggccagtga 186540 tgatgagcat tttttcatgt gtctgttggc tgcataaatg tcttcttttc agaagtgtct 186600 gttcatatcc ttcgcccact tgttgatggg gttgttgttt tttttcttgt aaatttgttt 186660 gagttctttg tagattctgg atattagccc tttatcagat gagtagattg caaaaatttt 186720 ctcccatttt gtaggttgcc tgttcactct gacggtagtt tcttttgctg tgcagaagct 186780 ctttcgttta attagatccc atttgtcaat tttggctttt gttgccattg cttttggtgc 186840 tttggacatg aagtccttgc ccatacctat gtcctgaatg gtattgcctg ggttttcttc 186900 tagggttttt atggttttag gtctaacatt taagaagaag gatacttaaa gtataaggga 186960 aaatgttaca atgtatgaag ggaacatgaa gaaatagaat ctggtaaaaa agagttcttg 187020 cttttgggag gccaaggcct cctggctaac atgatgaaac ctcatctcta ctaaaaatac 187080 aaaaaattag ccgggcgtgg tggcacacgc ctgcagtccc agctgcttgg gaggctgagg 187140 caggagaacc acttgaaccc aggaggtgta ggttgcagtg agccaagctt gcaccactgc 187200 actccaggct gggcaacaga gcgagactcc atctcaaaaa aaaaaagaaa aaaaagagtt 187260 cttgctttca aaactatgga ttaggtaact tttgtgaatg agtaagatca tgagtattat 187320 aaaaatagca cctttctttt ttgtcttggg gaaattatct tattttttaa ttggatttca 187380 gaaaagagta tttcagagaa ataaatctct gaaatgcttt ttgaagtgtg aaagatttag 187440 aagacaaaag caaacctcct gtctagataa acattaaaga gatctgccct cccctcctct 187500 acctattcag gttgcaacac tttgggggtg gctgccttgg tagagcttga tcgtgactct 187560 ggtggcttgg gagatggcat gctgcacaag ggattcatgg ttacagcggg cttgtgggac 187620 tggggctctc caatacgtgg ttgggtttgt aaagaaatca gagctatggt gtgaacaaaa 187680 ggatatgcat gggagacagt gagacaagga aatgctccag aaattattgg aatataggtc 187740 agataactaa ctgtacttgt gccattttct gggggaaaat tctctgaagg ctttttggga 187800 aaagaatgga agtgagaatt ctcaggtcct caaaatattt ccttttactc agtcctaacc 187860 tgaggccgtt aaagaattcc cagagtcacg atggaaggca tgtttgggag taagagccag 187920 agtgagggtt agaaatgtgt tgttggccag gtatggtgga tcatgcctgt aatcccagca 187980 ctttgggagg ccaaggcagg tggaccacct gaggtcagga gtttgagacc agcctggcca 188040 aaatggagaa acctcgtctc taccaaaaat acaaaaatta gccaagtgtg gtgacacgtg 188100 cctgtaatcg agctcttcgg gaggctgaga caggagaatc acttggaccc aggaggtgga 188160 ggttgcagtg agccaagatc atgccactgc actccagcct gggtggcaga gcaagactcc 188220 atctcaaaaa aaaaaaaaaa aaaaaagaaa gaaatgtgtt ttccagggtt ctgggtactt 188280 aggaatttgg ttgcttttgc aggtggaagt ggaggtgact aggtaacagc tgagtgattt 188340 tgccccagtt ggacatgagc caggttgagc agaaagccct gggatgcggg gaggggggtg 188400 gcggggaagg aattgaaagt tggttgtgtg gtttggcttt ggcttcatgg catgctcaca 188460 ccttgcttcg catagcatgc ttagactaca gcaggagcat caggaagtgg atttctgagc 188520 tcaatacaaa aagttataaa taccacctat aagggcaata aagatatata gttgattttc 188580 ttctttgcaa ggccaaatct tataggaaca taagagcgaa tgagttacag cctgggaatt 188640 tgagccttat attcagagat tttaggttgc ttctgattcc gctgtctaga caaaaccatg 188700 agaggatagt gtctagaaat gagaggaagc tcttccaatg cagaggctag aatgtgtcag 188760 cctgtgctgc gaggcctggg atagatgttt ctgaaaagta aaagggcagc tttcctactg 188820 gatacttgat cctcaggctc tagaaaactc tgctttatta actttgttga cttcctaggc 188880 accacatggg atccttgttc ttcctccttg taagcagtaa ttgaaatcag tttggcagcc 188940 tggtttacag tgaccatggt ggcttgtctc ccgtgctctt acctcactct gttgatgttg 189000 taaaacctcc agctaacttc atggggtggc tgacccacgt tgctcattta ttcattcaac 189060 acatattcat tgaccatcta ctctatgcca ggtattgtta tcagcactgg gaatagatca 189120 gtgaactatt gatctatttg tctaatggga caaattgaca aattgggaaa gattccatta 189180 cacaggtgac atttaagcaa agtcttgaat aagggaggga atagtaccat gagatatcct 189240 ggtgaaaagc aatttaggct gagggcacag cagggaagag gccctgatgt gggaacatcc 189300 ctggtgtctt gaggtacaga ggccagcatg gctggcacgg agtaagaagt tggaggtgcc 189360 gggcatggtg actcacacct gtaatcccag cactttgggt ggctgaggca gatgggtcac 189420 ctgagcccag gagcttgaga ccagcctggg caacatggtg agaccccatc tctacaaaaa 189480 aatacaaaga aaattagcca gatgtggtag catgcatctg tagtcccaat tgcttgggag 189540 gctgagatgg gaggatcaaa ttacttggga ggctgagatg ggaggatcac ttgagtccag 189600 gaggtggagg ttgcagtgag ctgagatcat gtcagggtga cagagcgaga ccctgtctca 189660 aaaaaaaaaa aaaagaaaaa gaaaaaagaa aaaaaaagaa gttggaggtg agtaaggaga 189720 ggaacgtggg ggacagagtc ctcaggactc tggcttttac tctgagtgag tcgaaaatcc 189780 aattaaaggt ttgaaagaga ggaatgacct gatctgacat tttattgtga acgttttcaa 189840 atctttacag aagtggaaga gcataacgat ccttcatgta cacatcgccc agcttcaact 189900 atgatgtttc atttgtaaat atttccgtct acacttccaa aggatgatga ctatttttaa 189960 aagtccaact ataataccat tatattttaa aagttaaaac actatgtctt taaatatcaa 190020 gagtttgtat tgattcgcac tttgaaggtc gagctgatga aatttcctga ggggttggat 190080 gtgacatgag agaggagtca agtattgcat ggtaattaaa aacctttgca gcatagtcca 190140 tttaccgaaa gactatatgt atgcacttca aagcaggttt taaagattaa catcaagcat 190200 ctggcttcat gagttttaac ttcttttcat aaatgttata caatgtcatc atctctccag 190260 ctagagaaaa tgctattatt cttattttca aatgaggaaa atgacgcaga attatttaca 190320 tattatgtaa cttggtccca agtcccttag atactggttt agaaaatcct agtaaactgg 190380 aagtgactta tccaaaatta aaatttattt tgctctattg tcttttgttg cctatgggaa 190440 ctttgtgcag gtaactaggc acatgtcagg actgatttac tgacctctca aggtatcttt 190500 aattattttg ggggatatca cggaatgagt tctacacaat tcatttgaat cgaattgaac 190560 ttaagaaaat tcaaatgatg cattggctgc ctcctattta ttacatgctg ctcataggca 190620 taacagcata gtctaacaag tataaaacct gtgtaactgt agctttcagt gcagtgtgat 190680 gagggctgag aagatagtgg tacaaagaag agaggtagca gagtgaagct gagtcaatat 190740 gatgaagatt tctctagact tgaaagggct agaaaaggtt attcttggca ggaaaaaaac 190800 atgagccaag gcataaggat aagcacaggc atggcagatt tgggaatgtc atgtaatttg 190860 ttgctgggct gcaaagtaca tggaagggga gtgaaggaac agaaggagat gaatctggag 190920 ggagaggtta aagtgttcca gagagcaata tgtaggtgtt actctaagtc aaagaggtcg 190980 taatagcatg tccagactcc aaaactctaa acaagtcata gaattgctgc cttggtaggg 191040 catatcacac acatcaaccc aatcctctgt caccatgaca tccatataac tgcaactcta 191100 tacatttccc agcctatgtt cccagagtct ccagatgaca ttgtctgcaa actgcactgc 191160 agaaggctct gctatgtctt cttaaaagta agcaagactg ttttcctttg ttacatgagc 191220 agcaaaagga tagggtgctc tttgacctca cttactgtag ggtggatagg aaagtcaagg 191280 aagagtaacc cagaagattt agttttaact ttcgcatcaa agaggtccct tagcatctgc 191340 tcagagatgt cacaatttct ggtgtgtgat tatgtttaag aattcggcct tgccactgtt 191400 gaagttgttc tgtggaaaaa gaacctctct taattttaca tgatgcccaa cttctctttt 191460 attccagaat cactcatatg ctgttggact ctttccagcc atgtgtgcta acctaggcaa 191520 tgtcataata gatgaattat gtttactttg tctttgatat ctcagctctt ttatcttcta 191580 ttcaagttcc cacctccatc attactgata gtgttcgttg aacaaagaat atgtcagata 191640 tacagaagtg tttctcccct tttctctgtc tctcttttcc ttcctttcct ttagtttcct 191700 ttctctgtct gttttctgat gcctcatttt agaaaagtga ttttttttgt gggaaaatca 191760 ttttagcatt agaaacgcaa tggctatcac tgacagcttc ctctgatgaa acggccattt 191820 gtcatcatta cacggtcatg ggagtgctaa gaagacttaa atgcagggct accacccctt 191880 cccaattcat cttttatcca ttttatttct ctaaggaaag ggtttgaaaa atgggctttg 191940 ccctcttgga tgcagtgaag aaattctagc tggctacaga tgttattgtt ggtcggaggc 192000 aagggataaa atcatggtca caccattgta gcgccagatg gggaatgtag caaacatagt 192060 tgtaatttct cattttacag atgaagaaac tgaggtgcag aggggttcgg tgacttgttt 192120 aaggcatgta attgttattg gcagctttct gttcagaact taagagtatg agtcagtcta 192180 gatcttttca tcacaatact ctgctcctct tactttttcc tgaaatttgt cacattgaca 192240 gcaatgtgat ccctaatgac acacagattc ccaaataatt tttgtagtaa aaatttccat 192300 ttgcaattct ggacatgtgt gtgtgtggaa ttttatgtga tgacatattg gtcctatctt 192360 ttgaatagga tcataaatga aatgacttat ggatcacatt caaaagcagg ccaggggcca 192420 atgtgtaagc a,ggtgggttt tcatatttgg agttctgtac ttttgtgtta gtcagtgggt 192480 ctaggactct tgtagtgtat ttcccaaggg ccaaagtctt ctgccttgag gtgtcagctt 192540 tccaaggcag aggctggatg ctttctcttc cttctgggct cctttctctt aggcttcccc 192600 cttctcttct cctccatttg tatctgtcct ttttctcggt actttccctg gctggtctca 192660 gctagatgct cactcaatgc tgttgaataa atgaatgaat ttcgtagtaa ttctgcaggt 192720 aaatcaagtt attgtctccc aatacggtgc tatgctttct gaggaaatta gactggaagt 192780 caggcttttt aaaaaagaag atgtggtgtc aaattgcagc tctctctctc tctcgactta 192840 ccttttttct atcatccata tgccttcttt cttgtatctt tgggttccca gacctcacca 192900 ttcattagca cttggaatgg attggtaaga ataaagaaag gagaggtggt gaaactcagc 192960 ttgggtcatc tggttacaca ttagtaactg acaaaagata aaaagataca gactaaatgg 193020 gtttttaggg aactttttcc agtctattct tgtttcccat tagtgtgaaa aatcaacact 193080 tgcttgtatt ttggggtgaa gacatttttc ttaagtgagt gggaaagcct cttgacattt 193140 taccgagagc cttaaatttt gaatggtgaa tgctaatgtt ctttgtgcat aaagaatttc 193200 agaacttgta tatatgagca ttaatgatgc atcattttct atttgtgagt taaactaggt 193260 attatctgta atcatatttt taggaaacat tcaaactttc atcaagtcat tctcttatat 193320 gactctcagc tccattaact ctgttttcat ggaactcaac agagttctta acgtttgcat 193380 tataaattaa attagcattt cccctcaaag aagtattgct gtccttacaa taaataattg 193440 tagacaattt cttttctttt cttttttttt tttttgagac aggttctctc tctgtcaccc 193500 atgctggagt gcagtggcac agtcacagct cactgcagcc ttgacctcct gggctcaagc 193560 aatcttccca cctcaacctc ctgagtagct agaattatag gtgcacacca gacctggcta 193620 atgtttaaat tttttgtaga gttggggtct tgctatgttg cccaggctgg tctctaactc 193680 ttgggctgaa gcattcctcc caccgcagcc ttccagagca gtgagattac aggtgtgagc 193740 taccatgccc agctaattgc aggtgatttc taatgggatt tagtatttct gggtttaagg 193800 atgagatctg aggtaatgac tttgtttcca gatgtgaaat aatttgctct tgggttgtga 193860 gccctttggg tgggctccca aggatcctgc tctcttccag gagcccaggc tctggggtca 193920 gactgcctgg gtccttgact ccctgttttc tgattgtaca actttggtga gtggcctaat 193980 tcctctgtgc cttggctacc ttggttacta tttctaaaac aactggtgtt gtagtagtac 194040 tgcttagagt actttcaagg gttaaatgaa ttaatccatg taaaacgctt aaaatagtgc 194100 ctgccacaac catcaattta gtgtgaaaat ctgctcacct gcttggccag cccctttcac 194160 tttattaaac caagggtcgt gctgggtttt ccagaagtct aagttgcggt ctaatctttg 194220 tgcagaagct gaaatagcag ccataacgtt ctccctagat gatttcgtgg agcttctttg 194280 aactgtatct atctccagtc atttttgtgg aagaaatttt cttctgtact ttttagggat 194340 gagaattacc tgccttggtt tattaactaa aagacaccat gattacaaat aaaattaaat 194400 aaatattgta tcactaaata gataatatga gatagatgta ttaagttttc agataaacag 194460 tataaaagag ctagagtaat ttgtaaaaag ttgggaggac ctattttgtc atgcaggaaa 194520 caatttttaa cttgcctacc ccagaacata gctaccacat ggttagggtt tgcccaaacc 194580 tggcccagga gtcatttacc ttgagctttc ctaaaaagga ggatcaggat tttcctctcc 194640 agactctatc attttaggta gagtccttct tgtcaattct ttttaagaac atacatttac 194700 ttttgtggaa aataaataga tacaaaataa atacatacaa aattgcatag caattagaaa 194760 tacccaggag gtatgttatg gtcacagaca caaactgcct ccaacttctg tccatccata 194820 gtgatattta aagcagagag aggtacacag gtaaccacat ttagatggac tgggatgttg 194880 ccacacatac aagcattgat aactggcttc tcattacctg aatacattct tctgtcagag 194940 caacagactc agctatgctt ctggcaaaat tgttcttaat tctctattga ttaatttatt 195000 cggtaagtat ttattgggta ttttctgtct gaaaagtgcg attccaggtg ctttatgtgt 195060 ctctgtgtgt gggtgttata taaatactta taatactgta tccatactct tgaaaagctt 195120 agttgggaag gcaaggcatg caataaggaa cacagaattt tagtcattcc acaaccatct 195180 gttgaatggc tgctattgtt agtatcgtgg tggaaactga gaagcaaaga tgactataat 195240 aggatctctt ttctggagat gcacagtgga cacgtagtta tatgatgatg ataaggactc 195300 cagaatagtt ctatacatga tgctctgggg ccacatgcag attctgatga gaaacaatta 195360 actctttttg gctgctacct gagaaggggt aattgtcact caggaggttt ttgccttttg 195420 accaacatag aaaggagtgt gagtgaaggc tagaggtgta ctaacttggt cagggcaggg 195480 tgacacataa aattaaccat cacagggaag ggtagggctg gagaggcaga ctgtggccag 195540 gttacaatgc gctgaggcta aggagactgt gtttatcctg taggccagtg ggtcttactc 195600 tgaagtcttt tgggtgggac attcatggac ttcaagagac ctgtgaatgc cctaagatta 195660 taagtaaaat ctgtgagtct gtaactaaag ctaaagctat ttttctgggg cccaccatct 195720 aaagaagatt ctgaagcctt agggtagccg tggaggagac atgaaggtcc attttgcatg 195780 gtagaaccct gcctggctct tgctgcagtg tgggaggaca ggtttgcaat gtggaggtgt 195840 ggcaggcatg gatttgggag gattggcaga ggactcacca tgtccataca ctcactgaga 195900 tggcaaatat ttattaatca tccaactgtg tatcagacac taagaataag ctgggaggcc 195960 atggcaagtg aggtcaccac agtccctgcc acagtggagg ttatggtata caggtaaggc 196020 agggaagagc actgcaaagg gtttgcccat tgcatcagtc atttatttat gcacatgttg 196080 attcaacaat tatttctatg ccaagctgtc ttcaaggtgc tggaggaaat gaagcgtaca 196140 tttcactggg gaagacagac aataagtaaa cacattaaaa tctggcttgg cttgatgttg 196200 gggaggggtg agtgccatag agaaaacaaa ccatttatgc agccaacaaa catatgaaaa 196260 aaatctcatc atcactggcc attagagaaa tgcaaatcaa aaccacaatg atataccatc 196320 tcacgccagt tagaatggtg atcattaaaa agtcaggaaa caacagatgc tggagaggat 196380 gtggagaaat aggaacactt ttacactgtt ggtgggagtg taaattagtt cagccattgt 196440 ggaagacagt gtgatgatcc ctcaaggatc tagaaccaga aataccattt ggcccagcaa 196500 tcccattact ggctatatac ctaaaggatt ataaatcatt ctactagaaa gacacatgca 196560 cacgtatgtt tattgcagca ttgttcacaa tagcaaagac ttggaaccaa cccaaatgcc 196620 catcaatgat agactggata aagaaaatgt ggcacatata caccatggaa tactatgcag 196680 acataaaaaa ,ggatgaagta atgtcctttg cagggacatg ggtgaagctg gaaaccatca 196740 ttctcagcaa actaacacag gaacagaaaa ccacacactg catgttctca ctggtaagtg 196800 gaaattgaac aatgagaaca catggacaca gggacgggaa cattacacac ctggggtcta 196860 tcagggggtt gggggctaag ggagtgatag cattaggaga aataccaaat gtagatgacg 196920 ggctgatggg tgcagcaaac caccatggca cgtgtatacc tatgtaacaa acttgcacat 196980 tctgcacatg tatcccagaa cttaaagtat aattaaaaaa aaaagaaaag aaaacaaacc 197040 agtgtaagag gatggaaagt aataggctcg tttagaatgg tgtgagaaag ccaggcaggg 197100 agaaggcgct gagacaggga ggtcctggat gtgtttgtgg aagagctgtg gcagcacctg 197160 gaacttgggg agcaagggaa ggagtgtggg caggcaaggg tgagggtgca gggggtcatg 197220 ctgggccttc caggtcacgg aaggacttga gctttactct tgttgtggtg agaagctgct 197280 gagggcttgg agttagggga gtgaaaagat ctctactata atagggagag ttcgggatct 197340 gtaacttaac cccaggagcc agcaaagctc cctggaggaa atgcagttta agctgagaat 197400 gggaggataa acaggtgttt ttcagagaag aggaagggtg ctctaggcac agagaacaac 197460 atgctggaat gcttctacta gatcataggg gcaaaatggg agtgcaggag taggagaggg 197520 ctttctggga aagatactta ttttaatttt gcatgcattg agtttttgag gtttctttgg 197580 tttgttcatg tggaggtgca gagtgggtat ttagcacata ggtctgaagt ccaggggagg 197640 ggtgtgggac agcagttgga tgtggcagag attccacaaa gagcaaatat catctgagaa 197700 tggcagaggg ctgagggcag agccctgagg aacactggtg tttaggagcc tgctggagaa 197760 agaaaatact gcaaagggaa cggaagtgga gtggttgcca gacatagaag ctagtgtcta 197820 actagatgtc atgagatgtg gggaaggtgt tacgtatcta agaatgcaaa gttgaacccc 197880 tgtgaactgt aatacttaag ataagtcgta taaattgtct ggaactagag cttgattttc 197940 caggagagat gaaatgtgtg taggtgacag gaaacaatga atatgtgggc gagtgtagtg 198000 tgagcaattt ctcagaggtg aatttgacag cattttgctt aggaagctac aaagagacca 198060 atgctagttg gtgcaaggaa ttcaagaatt tggacttaag tctatataat gatgattttt 198120 tttttttaac ttgagtttcc cggtttatca ctcccagaat ataggcagaa gtttgagatt 198180 t'ttatgtgta ttttctggaa aagatagttt cagtgttttt tacattctca aacaggttta 198240 tgatccaaag aaaaggcagt ggtcacagat acatgaaacg acaaggtatt caaaggagaa 198300 cgttgtactt tatgacagtt ctttgggcag tggcttgcag gatgagtttg aggaatgatt 198360 ggaggcagga gagtaattct agtaattcaa atgtggagta ttgttgatct ctcagacaca 198420 aatggaaaaa caaggaattc aaagaaagat aggcagagtg ttttgaagaa ataattgatg 198480 aaatttggta atgagttaga tgtaggagat atatttagca aatatttatt aaggactgta 198540 ttaatctgtt atcatgctgc taataaagac ataccaagac tgggtaaatt ataaagaaaa 198600 agagatttaa tggactcaca gtgccacgtg gttggggagg cctcacaatc atggcataaa 198660 gcaaaggagg aacaaagtca cgtcttacat ggcaatagag tgtgtgcaag ggaactgcca 198720 tttataaaac catcagattt catgagaaat attcactatc atgagaacag cacagacaaa 198780 agcctgccac catgatttaa ttacctccca ctgagttccc ccaggacaca tggaattatg 198840 gaagctacaa ttcaagataa gatttaggtg gggatacagc caaaccatat caaggaccta 198900 ctgtatatgg ttaaaattgg gagcaaatga gacatgattc ttgccttctt ggagtttact 198960 gtttactagg ggaacataca cttgtcaata atcacccaaa tataggattg gaaattgtgg 199020 taagtgccat gaaaaacaag tatagggaat tttgagtgta catagctttg gggacttgat 199080 ttgatgaggg agccttatga agttattgca ctagaactga attaaaccac atttctagga 199140 agtggacatc tatttgttgg ttctttaaat ttagctttac agaaatattt cctttaaaaa 199200 ccaaggcttc ttaaattttt aaaactgctt ggctaatcag gggaataatg cttttggata 199260 gctggtatcg ttatttatgg ttggaaaaac aacagtattt gattacattg agctttaaac 199320 ttttcctttg attaatgaaa attttattgg cccatagttt ttattatgct ctgtttttac 199380 ttggtccaag agattctatt ctctggaccc aatatgaata ccttcagaca tccctctttt 199440 tttttttttt tttcacccag gctggagtgc actggcacga tctaggctca ctgcaacctc 199500 tgcctcctgt gttcaagcaa ttctctgcct cagcctcccg agtagctggg attacaggca 199560 cctgccacca cacctggctt atttttgtat ttttaccaga gatggggttt caccatctcg 199620 gccaggctgg tcttgaactc ctgacctcat gattcaccca ccttggtctc ctaaagtgct 199680 gggattacag gcatgagcca ccacacccag cccagacatc cctcttaatt atgttgaata 199740 tgtaatatcg gtgatttcat ttgaaaatat ttagtagtcg aactagatca aggcagttaa 199800 gcttcctatt tccatagatg cagtggtatt gtgtcttttt tatatgatct ctcatgcttc 199860 tggacatcct tttttctgct attcttcatt ccttagctac acttggtgct tcgtggttgt 199920 aatgcattgt catagatgcg ttcatttctc attcgatctt cagctctatt tctttccaga 199980 gaatctctac aggcatctgt taggttgaag gacatctaat gtcttaatgt gtagcttggt 200040 aaaccagtca actttctatc tgagtcttaa gagaaagtgt ccaagatgag aaacggtaca 200100 ggtttggtga caactcagtg agaaaaagaa gaattttaca aggaaggagg tatcttagta 200160 attttgctaa agaagtaggt aaaccttcac ttataataaa gggatagggc tcggttaggg 200220 tttgtgaagt ctccccttag gaaagcaaac cctgaaatat tttgaatctt ttaaagaagg 200280 aaaataagag tcttttaaat aaatttttaa aatttatttt atatattttt tatagacagg 200340 ctctcactct gtctcccagg ctggaatgca gtggtgcaat catagctcac tgcagccttg 200400 aatgcctggg ctcaagcggt ccttctgtcc cagcctcctg agtagctggg actgcaggca 200460 tgagccaatg tgcccagcaa gagacattca ttttggtact gtgatggtac agaaaaacaa 200520 agggcctttg aggccgaagg agcagaagaa ggatggactt agacatggta taggcacttt 200580 ctactaaaga gctgtgaagc taaaaatgcc aggtctatga caggtgcagt gggccaaggc 200640 caggtagaga gcagcaggaa gagaggaggt ggggacctgt acctaggccc atctgctggg 200700 actgatctag ccataggtac tcagagaagc ccagattggt gcctgaccca cccttatggc 200760 ccagacatgg acacctccca gtctgttcct tcctgctgcc catggatggg ctgtgttagt 200820 ctgtattctg aggacacagc tctctgtcta gaggaagtta tgttatcttg atctgatgga 200880 tactcaacgt gaacattatt tcaacgtgcc acagggtctt ggagcccaga ggaagaccgc 200940 tcttgccttt tagtttatat tctttgtttt tttttaaata acattttgac agtctttatg 201000 gagtaagtct gggccaaaat gataattgac aatgttattt acatggattt ctaagttggc 201060 taaaaaagtt cctttatggt tagtgaatat agcccatgta gtttccccgt cttctttaga 201120 tgccttctat ttctatgccc aaagtctgca gttgattttc agtaagctgg gggtcatctt 201180 agagataaaa tgtagatgaa tggcattttg ctgacagcat acatctttgc tatttctgag 201240 gaaaatgggc tctcgctatt aaatcttttg tcaatattta taaaaatagt atttacatat 201300 tctatctata ttgtggaaac tatacattta ttgattcagt catttgatat caatgttgtt 201360 gagtccctat tccaagtgag gcactatgct ctaagcacat ggcattttaa agatgaataa 201420 gacaccaaga actttgcaga tagtaatgga aatgagaatt aatcaattga agattaatat 201480 agtaagtagc agaagagaaa taaaaaaatc ttctagagag ttcagaacag ggatgttgat 201540 tcaagtttat ggggattagg agtggctggt aagggaggca ttcaggcaaa agacataaaa 201600 atgcagtatt cccctcgcac tcattaggat ggctactata ttagaaaaag aagagagtaa 201660 gtgttggaga ggatatagag caaatagaaa ccttgtgcct tgttcatgag aatgtaaaat 201720 ggtgcagcca ctgtggaaaa cactggtgat tcctcaaaaa atcaaaatag aattatcata 201780 tgatccagta attctacttc tgggtatata tctaaaagaa ttaaaaatct gggtcttgaa 201840 gaaatatttg tatactcata gttatagcaa cattattcat aatagccaaa aagtagaagc 201900 aatccagatg tctatagatg gatgaatggg taaacaaagt ctgtgtagta tatacagaca~201960 atggcatatt agtcacatca tggaccttca ggacattatc ctaagtgaaa tatgctagac 202020 acaaaaagca aaagtagggt ttcacttaat gaggtatcta gaattgccac attcacagag 202080 aacaaaagta gattggtggc tgctagggga taggggaagg agaaaatggg gaattattgt 202140 tgaatgggta tggagtttca gttttgtgaa atgaaaatgt tctgaagact ggttgcacga 202200 tgatgtgagt atatctaaca tgattgaatt gatgaacact taagcgtggt tacgatggta 202260 aattttgtgt tatatatatc ttaccacaat ttaaaaaata tagcatttta ttatgtaggc 202320 gtgggtggga agatacttga cacattggaa cttctggcca tgcgtatact gttcactcac 202380 ttattccttc attcattcaa caaacatgta ttgaatgctt gctatgtgct gggcactgag 202440 ctagatataa caattaataa ggcttataag acattgaatc tatcaatttc atgcttgcta 202500 aatatctact cccacctcca aaggcactaa gcttctacag ttagatattc atagctgctt 202560 cctactgact tgaatcatgc ataggatatt agtaaacaag caataaaaag atttgaggtt 202620 gatgggggtg ggttcaacag catggtggtg aaatggaaag agatgggtaa cagaatatga 202680 actagaattg aaaactgtga gccagtgctc tctaatgaac attaaaaaat aaagaattcc 202740 tatttgaggc tgccaacctc agaactaagt tatttagaat ggacgaaatt ggcaaagtca 202800 gacgtactca acccaaggag ccaatatttt gtgaatatta tggcaaatgt agtttgagaa 202860 ccactaccac aaaattgtga accataataa tgactgagaa ggcagggaga ggttatacaa 202920 tttgggctaa aaggaaagac agggcttgtg aaggggagcg ccagtgaaag tcagtgtggt 202980 tcgggtattt gggtggggac tggaagcagg aagcttgagc ttcctttgcc aagagaccct 203040 gctggaaggg ctatcatcaa ttgactttag ctcatcttag gattttcatt ttttaaaaaa 203100 tgttcacagg aaccttcact ccatctatac tttcaatgtc tgcctacctt tctttcttat 203160 acaactttga acactctctc cattcattta aatatattat ggagtgccaa ctacatgcca 203220 ggtactgtgc tgggctctta ttccaccttt atttgattgc acatgcctgc caagtcctgg 203280 gccaatataa catctactcc tatgtctggt ctggcgagag atgcaaactc atcttcctct 203340 actttcctta cctccttcct tccagtcttc ttcaagttgt cttcattgag gcaatttctt 203400 ttacctgtgt ttttaatccc aactcctcta gtttccttct tggctttatt cttttatctt 203460 cctctttgtg ctttcaaaca ttccctttct cctggcccat gcccttcagt ctacacgagg 203520 ccttctcaag tctcttcatt ctaaaaaatt cattttcttg ggtcttatat tcttcagctg 203580 ccaccctatc tgtatctttt cctattctcc tccaagttct caaaggaatg ccttccctca 203640 ttttcatctc cttacattcc atctgctgaa ttttggcttg tgcctgtacc tgtctaagga 203700 aactccttgc taagagtctg ctttgtcagg tctgaattca cttaaccagt ctttgctttg,203760 ttggacttct ctgccccatt tgccattctt gatcatcctc tccataaacc tttctactta 203820 aagcatttta cttccttatt ttcttggttt tcctagaatc tccttactgt tcattttcag 203880 cttcctttct gtgttcctct tctcttccta catttttttt tagctttcta ctttcttaaa 203940 gcattttact tccttatttt cttggttttc ctagaatttt cttactgttc attttcagtt 204000 tcctttctgt gttcctctga ttgtctctct ttctacattt tttttttctg tgttcctctg 204060 attttcacgc agtctggagt tgtcatgatc aatcatagcc tactgcagcc tcgacatcct 204120 aggctcaagt gattctccca cctcagcctt acaagtagct aggactacag tcacacatca 204180 ccattctcag ctaatttttt taagaagcat ttttatagag atggagtctt gctatattgt 204240 gcaggctggg ctcaaactac agggcttaaa caattctcct gctttggcct cccaaagtgc 204300 tgggattcca ggcatgaacc accatgctca gtctctacat gttcctaaag aggagttttg 204360 aatattgaag aacagtattt tcaaattaca ttattcaagt tataaaaact gatatccagg 204420 gttatgtggc aatgacgtaa aaatttgaat tgttattttt ttgacacatg ttctgtgttg 204480 tccatcagtt catctgagtt ccaaatgtcc cagctgtttt atgctttgtc tctgtttccc 204540 agagaccctg agtgtggtct agagttggga tgagcattgg tctctaatgg ttctgaaata 204600 attgtatatt cctgcaaaaa cattaagtct attagaaacc agctaatttc attttgtcat 204660 ttttataggt aacatattct ggtgcaggta gtatgttttt aaaacaagtt tgcaataaac 204720 aatttcccct caaggttaat ataataggca acaccttttg ctgcaacaga cggcaagagg 204780 taatgaaaga ttagcttaca ttatgattca ttatttcaaa atgtcaggat aaagtggatc 204840 tgctgcatct cccagagagt gcatgttttg cttttctaat gttaatggat ttactgtttt 204900 tttcccccca ggccaaattc agataatcga cgccagggtg gcagagaaag attggccagt 204960 accaatgaca agggaagtat ggctatggaa tctgccaagg agactcgcta ctgtgcagtg 205020 tgcaatgact atgcttcagg ctaccattat ggagtctggt cctgtgaggg ctgcaaggcc 205080 ttcttcaaga gaagtattca aggtaatagt gtgttgaaaa cgacttctat ttttgatcct 205140 atgagcagat cctaagagcc aaagcgactg aggaaggaag acatagaatc agccatttgt 205200 acaaaacatg aatccctagt aggtccacta gtatctttgg tagaaacatg gagaagagac 205260 aggatctcag gagaaggagt tgacacatgg cagggcagct gaggctgagt aattccgctt 205320 ccttcctttg gcaagactca atcagtcttg agcaactcta cagaagaatt ccactagctg 205380 gatctctgag gaaaaaagaa atgttgtctg tgccctgact ggggaatgcc agatggacat 205440 tcatgtttgg taggcaactt tgcctatatg atctggtata tgctgttaat tgtccatgca 205500 taattatctc tctactcagg ccttgtccag gcaaatattc tgttttgttc tagtttagct 205560 tgttctcccc tttctctctt ccatctcttt cttgtctcaa tggatgacag gatattttgc 205620 tatgagctga ctcagtggtt ggtgtcttgt aatggggaga tatcatcttt atcaaacagt 205680 tattaagtat ctacctgtag catttcattt tcccgcctgc ctccattgtt ttcttgtcta 205740 tagtttgcca attatagcta atatacggag agctatactt tatttctact ccagaaatgt 205800 ctctattatt gcattataat aggataccct ggggaaacac taatcatttt tactacctaa 205860 aatacctatg ctgaatatcc tttatctgat aggaacagag atctgacagc agcttaggct 205920 aaccaaattc attttttatc ttaagtgtgg ggcatttttc tctcttctta ttctttacct 205980 tttcagctta agtgaaggtt agtataaaca ctaagaatat ttctgatgga gttttcatgt 206040 gattccttct acaaaaaccc agatttaagt aacttgttga aaaccagagt ccgctaagtt 206100 aataaacact gattgaagaa gtgattctca tggactttct gtgatagctc tttcctgccc 206160 tgatatgaga tgaaagctgg gggatggtat atagtattta tttttccttc cgttgccagt 206220 gggacttttt tttttttttt aaaagctgtt catatcttaa tcgagtagca tgtgaggtca 206280 acatggtcta ttttaaaagc attttcttcg acacattgct tttaacatct tttagaactc 206340 tgctgtgaga cacatggact tttttgttgg tatttttata caattaatga tattctcaat 206400 agtaatcttt gtgtgtgtat atatatagaa ataaattcta aatgtaagtt aatatattta 206460 ttatttttct aaacatatat aaatatatat atgcacacag gctatttaat tttattagat 206520 gatgctattt taattcagaa aaaaatgaca tttatatttt gatttaggtt agtataagcc 206580 cttagaggtg ttttgacaac tctcttaatt tgtggtttta ctgtttattt gattttatat 206640 aatctaaaat accattgttt ttaccaagca tttaatttgg cagtgaaaga gcgtctgaca 206700 gaggtatggt tagtagatag gtctaactgc acaactggat ggattgagct gagactgttt 206760 cctcatcagt aaaaatgatt tgaagcagtg gttggcaaag tttttctgta aagggccaga 206820 taataatatt ttaggcttta caagggccat gcagtctctg ttgcagctac cgaactggat 206880 tatagcctgt aaggtgacct gtaaacacat ggaagtgatt atgtgctaat aaaactttat 206940 ttatcagaat aggtaacaga tcagccctgg cccgtggccg atccctgatt taatgtttat 207000 ttatctgatc taaatacctt tatttatgga agggaatagg ggatttttaa atctaaagtt 207060 ttgattattc acattttact gagaacttac tctatacctg attagatgtt ccgagagaaa 207120 taaaaaaaaa gtgtaagaca taatccataa taccacaaaa tttaaaatgt atttaggaaa 207180 tttatttgag gaagtaaatg tacttgttct catgatacaa tcagaaagta agtcagtatt 207240 gataaagtgt tacctgtatg agaaagataa ggaaaacaat agagagatgt aagaaatgaa 207300 aataccagtt ataaattaaa attattaaga ttgaaagtgg aaatgatctt cctccgagaa 207360 acaatggcaa tattctcaca aattttttac atcatttttg ttcagcattt aagataaaat 207420 tatataaatt cccataacat ttagtattgt ctctaagcat taagaacaga aaaaacagaa 207480 ggaaaatata tttctaaaaa tcaacgaata cagtgtgaga tgtttcattg gtatggcatt 207540 atctcaagtt caaacatttt gaaaaatgtc tgcttactct ttgatagtta aaaacaagta 207600 tctcagctgg cgtggtggct caggcctgta accccagcag tttgggaggc tgaggcgagt 207660 ggatcacaag gtcaggagat cgagaccatc ctggccaaca tggtgaaacc ccatctctac 207720 taaaaatatg aa~attagct gagcgtggtg gtgcacacct gtagtcccag ctacttggga 207780 ggctgaggca ggataattgc ttgaacctgg gaggcagagg ttgcagtgag ctgagatcat 207840 gccactgccg tccagcctgg tgacagagtg agactccatc tcaaaaaaca aacaaaacaa 207900 caccaccacc actaacaaaa acctcttatc gccgtcttgt atacgcagac cagctagtag 207960 aattttactg aaacagtagc ctataaaaat gcaattccac ttggtttcag aaacttcttg 208020 tgtatcatag tgtgaagtca cttatcttag gcttttaaaa tgggataaat attgagtcca 208080 aagttctgga agaagcctag aaagaaggca gagttattaa cttttagata tagggaggaa 208140 ccttaaaatt attcagttct tcattcattc acttattcat tgactagctt tactaacaaa 208200 gccctatgca agaccctgga aatgcaatga tagaaaaacc tggtccctac cctcacagaa 208260 cttgtgaggt aaagggggat acagactgat aaaccagcaa ttagatgatg gtgtcaagat 208320 agaggtgaag gcagtgtctt ataggatcca aactccactc agtcctggtg gtggttgagt 208380 ctggctatca gaggtttcct gattaaatct ggagggtgag tcaagggagc atggtgaaga 208440 aggagggaat gcatgtttag ccatgtgaat gagtccatga gtgaagacca ggaggaaagg 208500 cagagcgcgg ggaattctat gcgtaatatt taacaaaatt aatgtactgt taaacaaaga 208560 catttctggg ccatggattt aatcctagac tgtgtaaaaa ccaaptaatt gatttccttt 208620 atactttaaa agcatttcca tgtatttgat ttgtttgtgt gtataaaagg gaaataccac 208680 aacaagttta agggtttcta gttctgcttt ctcatcatag tcttgataac ttggaactaa 208740 aaagtttttg ctgaaattgt ctgtgactct ttataaatca cactgcccct caaacacatt 208800 taaggatggt gaagggtctg acacgtaggt gggaagttct gaagatgccg cagctctccc 208860 tgttttcctt gttacttaag aagagagcta gaaatgagtg tacatcagat tattctcatg 208920 ttctaagtgt tttggttgaa gaggtaaagt gtttggcttg aaagcataca aattttcatc 208980 cactacttag tgtacaaact tgattactta agagattgag taatggcctc cagtgaaacg 209040 cattcttttt aaaaagcaaa gtgaaggatg ctatttaagt cagaaggggc aaaattggat 209100 attttatgag tttattaatc attgcaggca tagaagtagt gttccttaag atgtgtttta 209160 gacagagtcc ctgggatgag ttatataagc agatctggtt gtagcttcag cagccagata 209220 ctacctttga gtattacttc aaggaaaaag gactccactg agctcactgc ttctctttca 209280 ttattatttc agaaggttgt gtggcgtaga gggggctcag gcctacctat acaccactag 209340 ctatgttgcc attttatatt atttctataa ggtgccaaca gaagctgctc atcagatcag 209400 acagacatag cccaggcaag tattgattta cagatgatct ttggccagga agacatggta 209460 tcagggtaga gtctggttat gggtcaatgc agtggggacc ttaggtccta caggtataac 209520 tgagagcctg atccaccagg ccttagaaag cttcagggtg agacagtcca gcaccctgga 209580 tagctccttt aacagctgtg gccggtaagc aggcacttat ttgctaaaga actcaagccc 209640 atttagctgg cttcatctgc tttgtagagc tctgttaaaa agagttccta tttctccaca 209700 tcctctccag cacctgttgt ttcctgactt tttaatgatt gccattctaa ctggtgtgag 209760 atgatatctc atagtggttt tgatttgcat ttctctgatg gccagtgatg atgagcattt 209820 cttcatgtgt tttttggctg cataaatgtc ttcttttgag aagtgtctgt tcatgtcctt 209880 cgcccacttt ttgatggggt tgtttgtttt tttcttgtaa atttgtttga gttcattgta 209940 gattctggat attagccctt tgtcagatga gtaggttgcg aaaattttct cccatgttgt 210000 aggttgcctg ttcactctga tggtagtttc ttttgctgtg cagaagctct ttagtttaat 210060 tagatcccat ttgtcaattt tggcttttgt tgccattgct tttggtgttt tggacatgaa 210120 gtccttgccc acgcctatgt cctgaatggt aattcctagg ttttcttcta gggtttttat 210180 ggttttagtt ggtgggactg taaactagtt caaccattgt ggaagtcagt gtggcgattc 210240 ctcagggatc tagaactaga aataccattt gacccagcta tcccattact gggtatatac 210300 ccaaaggact ataaatcatg ctgctataaa gacacatgca catgtatgtt tattgcggca 210360 5~

ctattcacaa tagcaaagac ttggaaccaa cccaaatgtc caacaatgat agactggatt 210420 aagaaaatgt ggcacatata caccatggaa tactatgcag ccataaaaaa tgatgagttc 210480 atgtcctttg tagggacatg gatgaaattg gaaaccatca ttctcagtaa actatcgcaa 210540 gaacaaaaaa ccaaacaccg catattctca ctcataggtg ggaattgaac aatgagatca 210600 cttggacaca ggaaggggaa tatcacactc tggggactgt ggtggggtcg ggggaggggg 210660 gagggatagc attgggagat atacctaatg ctagatgacg agttagtggg tgcagcgcac 210720 cagcatggca catgtataca tatgtaacta acctgcacaa tgtgcacatg tacactaaaa 210780 cttagagtat aataaaaaaa aaaaatttaa aaaaaaaaag agatcttagt tcttttgggc 210840 ttggggactc actcttgttc acttaaagtg gattggttct tacatttatt ttcatagttg 210900 tgtctggtca ccttctggct tgatgtatgc ccctatgtct ggaaaaggtt agagaatggg 210960 gtagaagtgg aggctgcccg cccatcatgt aactgtttta tcttttcaga gatacaattg 211020 ggatcctaac tctttggtct ccgtatttcc acacttgctc ttatacgatt cagtttccat 211080 gcaacagcag gagagatatt ttagatatat taatcaggtc ctatggtctt gagtttacag 211140 ctctcagtgt aaatcttact ctcaagagaa aaattttcct tctgcagctg aaaacattcg 211200 acatattctc tctcctattc cttctccccc attcactgta ctccaggcac accagctttt 211260 atttttattt attttttgct attcccccca acacgccaac atgttccttc ctcaggatct 211320 cagcacatgt ggttcccttt ttccctttgc ttgaaatacc cagatcttta cttggctggt 211380 ttcttgtcat tcagattctg cccaaagtca ctttctcaga agtagtgtta cccttccacg 211440 agtaatacta ctcttacttc tctttacagc tctcaaattc ttatgaagtt ttcctattta 211500 ttcatttgtt tgattattgt ctgtctgcat caggtaccgt gcaaacttgg atgtaaactc 211560 agtgatagct gatttctgtt cagcattgtt tgttgctata gctccagtgc ttttagtcat 211620 gttgggcacg tccctactaa atagtagaat atggcgaagg gtcagagtca tgatagatag 211680 ccggatgtgg tggctcatgc ctataatccc agcactttgg taggctgaga tgggcagatc 211740 acgaggtcag gagttcaaga ccagcctgac caacatggtg aaaccctgtc tctactaaaa 211800 atacaaaaat tagccagttg tgatggcatg tgcctgtaat cccagctact caggagactg 211860 aggcaggata attgcttgaa ctcgggaggt ggaggttgca gtgagctgag atcgcgacac 211920 tgcactccag cctgggcgac agagtgagac ttcatctcaa aaaaaaaaaa ataataatga 211980 tagataaaaa ctgctagagg ctctctgaga aggaagaaat gtcactgggt taggttggta 212040 agagaagtgt taatgcaagt ggtacatttg atataagcat gcaaataaat ggtagtttgg 212100 aggaaaattc cagaaatgtg aaacagtatt gagaaattga gtaagccagc tttgagagaa 212160 cacacggctt attgtcctct ccttctgttt tgctcctcag ctctctaaag tgggcattct 212220 ctagggtcct gtcttcaatc tcagcttctt tttcttcttc atccttctct ttccacctag 212280 tttcctaggg aattatatat atatatatat atatatatat aattttgtat atatatacaa 212340 aattatatat atatataatt ttatatatat ataattttat atatatataa aaaattatat 212400 atatataatt ttatatatat atacaaaatt atatatatat gtctggtaca ggctgaattg 212460 tgtctcccag aaagatatgt tgaagtccta atgctcagtg cctcagaatg tgaccttatt 212520 tggaaataat gtcattccag gtgtaattag ttaaggtgaa gttatactgt ggtgtaaccc 212580 acagtatact tccagtatat acttcagtat atgggccctt aatctaatgt gactgatatc 212640 tttagaataa gaagaaaatt tggacacaaa cacagagaag agaatgtcat atgataacag 212700 agagagagag agagaagaga gagagggagg agagagaggg aggagagaga gggaggagag 212760 agagagagag aaggagaaag aaagagagga gacagaagag aaacatctac aaagcaagga 212820 acaccaaaga ttgctggtgc ctaggaaact aatataataa cataatattt ggggcctaaa 212880 gtgttacagc tgcaccaata tctcccaaat cttgctccta gctcctgccc cagcctgatt 212940 ttctcagtga ctcccctgtg tttccctctg ccaccagccc ctccttctgc ctgctgtgtg 213000 tgacagttcc cttgccactc cctccggcat ccgtacttca aactggggag ttagttattt 213060 ccattgtttc ttattgcagc ctctggctct gacgtttcca gagctgttgc atagctctat 213120 tgagtctatc tccttaaatg cattttcatc cacttaccat tgctgagtta gctgcattac 213180 ctttcaccag gatgcttgca ataatttatt gtttccattt gccttctcct tcatctaagc 213240 cgtttgctta tgtctttttt cttgttgcga gacattctgc acattgccac tctattagtg 213300 gtcataaagc aaatcactga tcatgtcaat acgcagtaca agatccttta atgacctcca 213360 tagcccatgg aatggtcctt aaacaagagt tcaaggactg ccacaatctt gttctagcct 213420 atctttctag tcattttaaa gtcaccattt ttacttgtga aataggcact ccagacacag 213480 tgaattcctt gttcttctac aaatatgatg attccatttc tttgctggga atttctttcc 213540 agatttacct gtaaaaattc ttcgaaccct aatcaaaagt gactgttgtt aagcccctga 213600 agataatgca gaaattctct ctctcctggg acctcgatat tagtttattc cattgtattg 213660 catatatttg attgcccatg ttctgtccca tactgactgt aaagtcctta aaggtgaggg 213720 cccaatattc tcagagtcac tcaataaata aataaaagaa taaatggaaa ttaggatcag 213780 tttgtgggct ttagcaacac aaaaacatta tactttttca acatgggaga ggtatgatga 213840 aggagttttt tttttttttt gagacagagt ctcactctgt cactgaggct gcagtgcagt 213900 ggcattgtgt cagctcacaa caacctccgt ctcctgggtt caagcaattc tcctgcctca 213960 gcctcccgag tagctaggat tacaggcgtc caccaccatg cctggctagt ttttatattt 214020 ttagtagaga cggggtttca ccatgttggc caggctggtc ttgaactcct gacctcaggt 214080 gatctacccg ccttggcctc ccaaagtgct gggattacag gcgtgagccg ccgcacccgg 214140 ctgatgaaag agtctttaatJ gcagattaat ctggcagagg tatataggag ggaccagaga 214200 agggaaagaa tcaaagcgtg aagaccaatt tggctgatat tcaactagct tagatgtact 214260 aaaaatctgt actttttggt atttgtgaaa tggaaagaag gggaatagaa taaaggatat 214320 tataatgaaa ggatatacat tgcttgaagg taattaaata tgggttatcc aggagataaa 214380 agagttaaag aggttcagac atagactgaa tgaactgaga aatgaatgac ttggtcacca 214440 agaggaaggc cagtcatcag ggggtaggat aagttcaatt ctagacatgc tgcatttgag 214500 atgatagctg gatgtccaga tggaattatc cagcagccac agaaacagaa tcagctctct 214560 gcggatattc caggggtggg gatttgaatt aatttcctca attaatttta aagaaacttg 214620 atgaaaagaa tggtctgaat acttcttgaa ggttgcacat tattaataat ggagaaataa 214680 ctctaaaacc ttcctcttga ttttcataat aatataagca ttccctgaat cttaccaaac 214740 cttgtaagaa acactcttat tataaaaagt gtatgtgcaa agcccttcta aacaggaaaa 214800 tgataaatta gtcctacagg gccaaatgca gctctctggg agcttacaat tcagaaagaa 214860 catcctgcta ccagcacatt aagctgtaca aatagtaaac tgcagaaaca aatataagca 214920 tttttatgat gtccaaacaa gaaccaagca ggtgtttttt tttttttttt tgcagattat 214980 ttatactgtg gcagttcata gcctcctttt cggacccaga gcttgcataa tccttccctt 215040 atttctactt acgtgtttta ctctccatca tgtgttaaca tacatactgt gcaacagaaa 215100 tgactatgga ggctgagggc agcagagttt tagtgtgtac acatatgagc tgtcatgtaa 215160 ttttcaagtg aaagcctttg caatgaaact ttttaaaaga aagtcatggc cgggtgcggt 215220 ggctcatgcc tataatccag cactttggga ggctgaggca ggcagatcat gaggtcaaga 215280 gattgagacc atcctggcca acatgatgaa accccgtctc tactaaaaat acaaaaatta 215340 gctaggcatg gtggtgtgca cctgtagtcc cagctactca ggaggtggag gcaggagaat 215400 ggcttgaact cgagaggtgg aggttgtagt gagccgagat tgcaccactg cactccagcc 215460 tggcgacaga gtgagactcg tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aaagagaaag 215520 aaagaaagaa ggaaaaagaa agaaagagaa agaaaggcgg gcattaactt caggtattgg 215580 taaatttgct aggtgtttgg ctactgtttc tcatcagaga aatagaaaga cacaccatga 215640 aagtcaaggc ctgaaaacct cattccatgt aagaatgaca tccccagtgt taagtgcttg 215700 ttagtggtta atgcgatcct gtgaaactta gatgtgtttg tgcacacatg cacgcatata 215760 tttgaagaac tcagaagagt taaatcacag cctttcaacc tgtgaaatga cagtagttct 215820 tcttttttcc tctccctttg gctaagtcat ctttatcttg gagataatta agacaaaaat 215880 gcctctgaca aataaaatca gtatagaacc cctatttctt ggcagctttt gtggacacag 215940 ctgaagcttt cagaggtctt gaaaaaccat ggcaacaaat gcctttgaag ggtaagcaaa 216000 ggttccaaat gtttttaatc gctggtgttt tttctgctac cacttcaagc atttttcttc 216060 attttttgtt catctgatca aaattaaatt tccaatttcc ctactaagtg gttctgtcct 216120 ggtcatgcca ttgactattt ccattaaagt agtagagttt gtgcccacat atggttgtta 216180 agctcatcaa caattccatt agaaagcttt gtttatcagt ggcaataatt tcccataaaa 216240 attatagata ggttttaatg ggcacatttt caaaaggcat caactcgtcc tcaaaattat 216300 gtgctgacac tgttcttaca accatggttc gtggcctaat tccaccaaat ttctctcttt 216360 ttcatagaga actgttgtca gtagctttat atccttttaa agagaatggt ctgtatctct 216420 gatactcatt cagagaaatg agtattttag acgtaggttg ctaattttaa gctatatact 216480 acactatgtc agcactatat aggttatctt gtaacctgct tgcctggcta tttggctgaa 216540 aaataacaac atgtaaggaa aatctatttc ataagtctaa catttacttt gtaaaacttg 216600 ttctggctac tctgttaatt ttccacttac gtgtgggtta gagagcagat ttgatttttt 216660 tttaagcgaa agatatggct tacctgagaa aagaacatag tggggaaagc actcctatta 216720 ttttcctcat atttccattt tcctttagcg gaaataaaaa gacatttcag tttttcagtt 216780 gctaagaaat gaaggaacca aagacaaaac aacttaatta ttaaattaca atttatttct 216840 gtaataagca ctcgttctct ctgttttcct ggggaaagag tatgtggact ttcaatttta 216900 tccaaataag catcatcttt ctctgattag tgtggcagtt tcaaaatcat gtattaggaa 216960 gtacagagtg aatgagtaga gaatttctaa attagcaccc aaggttgggt ggctagatta 217020 tgtttataaa tatgaacttt tgtattaagt gcaatgattt aaaagaatgc ctgcatcact 217080 ttagggcatt tcattaagtg ctgtgcacaa tatttttcct tatacatcat aaaagataaa 217140 ttatagttca taaaatagta taaattccta attattttgt gcttttgaca cctcagagtt 217200 actaataagg gatttcgttt taaaatgata tttatttatt tatttagaga cgcagtcttt 217260 ctctgttgcc caggttggag tgcagtggtg cgatcttggc tcattgcaac ctctgcctcc 217320 caggttcaag cgattctcct gcctcagcct ccagagtagc tgggaccaca ggcatgggcc 217380 accacaccca actaattttt gtatttttgg tggagacggg gtttcactgt gttgggcaca 217440 ctagtctcta actcctgacc tcaagtggtc ctcctgcctt ggcctcccaa agtgctggga 217500 ttacaggcgt gagccactgt gcctggctcg ttttaaaata atttaaaagt atattttgcc 217560 accactatta accagttaag ccatgatggt atattataat caccatggag atggtttttc 217620 tctttatttt attttgtttg ttttctgatt gctagcatgc tgattactct tcctattcta 217680 caagtgcctg CaggCCagCC CCatttttgC tCtCttC3Ct tCatttttCa tttCtCCCtg 217740 tttcactctt catagcacat ttgtactctg tccacaccta gagacctccc tgttgaagtt 217800 cttcacattc tcttccctag agagggttaa cttgttgagc tcagagactt aaacttaaaa 217860 taaaatgtaa cagatatgta ttgaatgact attttatttt agctctagga gaaatgcaaa 217920 gatatatcat ccatagaccc tgaaatccag ctaagggttc tgcttataca caaggtgaaa 217980 ggttgtgata atgcacatta aacaatagaa gagttaaatt cagcactata agtgatgcct 218040 caaagcagca taatgggaaa aaggggtatt tcagaaaaaa atggtatgag ttcagaggca 218100 agagaaaaca gaatgctcta gattaaactc aaagacttta tggaggaggt ggtgtttcaa 218160 tagatacctt tccacagaac catgaagaga gttctactta attgtaagtg gcctgtgatt 218220 tgtcgttagt cattgcatgc tcaattctgc atgtgtgata actgtgtatt tttatggcaa 218280 aatcgcaatt acttttgcac caacctaata gaatagtgtg ggtttaaaaa aactatttgt 218340 aatatattcg aacatatttc ttatatatat gtttcaccat cattagcagt aaatctattt 218400 tgcatccaga gattgaataa tattttgttt ctaggatgtg agtgatgtac tattttaact 218460' tacttttgaa gtttgacatg ggagtgatat taataaaatc taaagatgct cctaaagagg 218520 agcaatagat aatgtaggta tagataatgt agctattatg gaggtaagtg tatttgatac 218580 tttttattat gatatgaagg gaaacaacta gtcccaagct acaatttatt aaggtaggct 218640 tagcttatac gccataattt ttatgtttgc aactttcttg attttcttcc tctggcttta 218700 cttctatggg atatttatga aaggatgttt ttaaaaatgg ctagagactt ttgcttctaa 218760 gagttgacat aaacttttcc acaacatgaa caattagtag atgatacact tagatacact 218820 cttgaatttt taaaattgtg gcagtaaaac tgatctcaga gaaggaagaa aatgctttgg 218880 ccagctttta tcaattatat acaacgtgtt tggaagattc agttcctctc gccttctttc 218940 tccctatata atactttttc ttaaggttgg tactattgat gcacttggtg taaacacatg 219000 agataatagc aaaatatgaa tctaggtccc agaaatgggc ttctttcact ctgccacctg 219060 ttctgactgc atttctcctg actttctgta aatcccgggg aggagctgga agagcaaggt 219120 gtgtgctagc ataactttgg ggatgaagta cccttcttt-t tttctgattt tgttcccaca 219180 tctccttact ggaccaagta agggcatcag tgtcaaactt cctgattgaa gatacatttt 219240 tgccctacca tgaagggctc tgggtaacat ttattgctta taaaatgctt ttttggtggc 219300 tactttgttt ctgaatataa ggcaagataa aaagtttgat tcaagagttt cttgtaaaca 219360 ctttgtgtgt gcgtgtgtat tcatatatgg ctgcacacgt acataagtcc atacataagt 219420 acagtccacc ctccatgttt gtgggttcaa catccatgga ttcaaccgac tgcaaatcaa 219480 aactatttgg aaaaaaatgg atggtagtgt ctgtgctgaa cacatacaga gattttcctt 219540 gtcattattc cctaagcaat ataacaactg tttacagaga atgtacattg tattaggtat 219600 tataaataat ctagagatgt tttaagctat atgggatgat gtgcgtaggt tatatgcaaa 219660 tattatgtca ttttatataa gggacttaaa catctataga ttttggtgtc tgaggagtct 219720 tcgaacaaat cccccacaga tcctgagggg ccactgtata tatatatctt cataaacaca 219780 cccacacaca caaacacacc cacccaccca cacacacaca cacaccaatg tgtatatact 219840 tttttttctt ttttttaatt cgagaccaag tctcgctctg tcgcccaagc tggagtgcag 219900 tggcgcaaac ttgcctcatt gcaacctctg cctcccgggt tcaagtgatt ctcctgcttc 219960 agcctcccta gtagctggga ttacaggcgc ctgccaccat gcctggctaa tttttgtagt 220020 tttagtagag gcagggtttc accatgttgg ccatgctagt ctcgaactcc tgacctcaag 220080 tgatttgcct gcctcggtct cccaaagtgc tgggattaca gatgtgagct accacaccca 220140 gttgcaatgg gtatagactt ttgaaatgtg tactacaaaa tattaacagt gactatctct 220200 gaatgatagg attatggata aattttgttc tttttgcata tctgtatttt tatttttcca 220260 cagtaaacat cttttacttc tgtaataaaa acttcattaa aaaatccatg ctgcaattga 220320 ttaattatat actaactttt ttttactctt ccctcagtac agaaagatta ttctgtattt 220380 accaaatctt tacactttat ttgcaaatca ttttgactgt ctgtttgcaa gaggtcaaat 220440 ttgcactcaa gttcagcttt cgttcattat ccatgagccc aagagaaatt gtgtcaggtg 220500 gacatcaggt agctgaggcc aatacaatat tattcctttg ggttgttgag aaatcatagc 220560 agaggaaagc ctgtgttgat aatagagtac caccaggaat ctaggtcttt acagctgctg 220620 gcctgaccag caagggtaaa atatttcagt cacgcagcca gtgattatta accctagctc 220680 tatattccag agaggaagag ctagcctttt tgggttgtga tctgtcactg attgcagttg 220740 cacatatact agccatgagg tctacacaca tgtgaagagc atgctacaca catagactag 220800 atacactgac tcatcagcct ggaaacagtg cctgaccctc actttgattg tttgaatgtg 220860 tcctgaccca ttaccctgcc tttggatccg ttcctgtttt tttttttttt tttaaagcat 220920 tttatctgtg aattaaaata gtactttcca ttgcttcatt tttttttccc tgaggagttt 220980 agctttgttt aattagaaac aagacaacaa tcattactgg tctggtgcaa tcttgtttag 221040 gactcctctt ttgcttagaa ctatacctgg attagggatc actgtgattt agagtgtgca 221100 gtatgaggtt atttaaatac ttaatagtat atttgaaatg aaaagagtag aaattaaaga 221160 ctgaatcatt taccaagaaa taacagagga gaccaattta cttctgtaag atttagtaga 221220 ggctgttccc ttctttataa ttggctttgt ttcagttacc agttaagtgg ggtgaactgt 221280 tattaaaagg ctatgggtaa ggttacagta gcattcttct aagaaaaact acaggagtag 221340 tcactgtgca gctatctgct ttgtacctgc caatatggca gtagatatgg aagggtcaga 221400 aaaacaggac ttcatgttca ataacttttc ctttttcctt tctttccctt atttttttca 221460 tgtcttgtat gcaagatagc atgcctattc tttaccttca atgtattttt gaatttttaa 221520 tccagatcat attttatcac aatttgtaac atgtagctaa taaaaatggt tattacatga 221580 gaacttgctt tacaaattat agatcaaatt tttatacaaa ttattatgtc accatagaaa 221640 aatagttacg atttatctgt ttagtcttag aaaaattatt tcacatatta gttaaggctt 221700 ttagttttga gtgagcaaaa tccaattcaa actagctttg ggtagaggtg ggggtaaaag 221760 agaggaatgg atttcaatgg aaggtttact tgcgtgtctt ggctaactgc aaacatggca 221820 gggtgcaacc agaccttagt gatcacagac ccagggactc agaccctgtg aggaccttag 221880 ccttaccgcc actcttctcc atgt.gtcagc ttcgcgttct ctgtctgcaa gtatgcattt 221940 tccctaaggt gggaagcaga gttggcaaca gctgccaggg ttgacttcct gtggcattct 222000 tgcagttagc aaatattaga gaaggactct gcttggccca gctttgggac acacctctgg 222060 accaactagc tgttctcagg aggcagtaac atgtacagag gtgatccctg gggctcgccc 222120 tgtgaaatca ttgtgagcca atcactacct ccctccctga tgctgatgtc tactatgttt 222180 gatgtctctg aacttccatt tcctcaattg tgaatggcac cctggttcct accttgctgg 222240 atagatgtag tgattagagt tcatgcttaa cctacaggtg ctctgtaaag aatcattggc 222300 atcattacct cttgttggct ctatcccatt ccctatagat atctttctgc tctgagacta 222360 agcagagaaa ataatttaac atttaattat tgtgcttcta aatccacttt tacctcttct 222420 ttctttactt ttctctttat tatttttaat tatttcaaaa ataaaattgt atatatttaa 222480 ggtgtacaag atgttttgat atacatatac at aatgaaat gattactgca gtcaagctaa 222540 ttaacatcaa tctcctcacc tagttatcat ttttgggggg catggagtga gagcacctga 222600 aatctctctt agcaaatttc cagtgtataa tacagtattg ttaaacatag tcatcatgtt 222660 gtacattaga tttctagact tgttcatgct acataactgc aactttgtac cctttgacca 222720 atacctctct attttctcca ctattccatg cctgaaattg tgggatatag gtatatagtg 222780 tgtgtgatat atatagaggg agtgatatat gtatatataa ttgtgtgata tatatgtgtg 222840 tatatacaaa cacacagaca tacacacatg tacatatgta tatacatgca cacatacaca 222900 atgggatatt attcagcttt caaaaaggac atcctgccat ttgcaacaac atggatgaac 222960 ctggatgaca ttatgttaag tgaaacaagc tggacacaaa aagaaacatg caccttactt 223020 cttagacaca aatttctttc tcatagccag cacctgaaaa tagggccaag tattttgcat 223080 gatttacaat tgactgtata aatgtagcat agccagttat atgatgtgtt accatttctg 223140 ctataaaatc aaaagaagag atcagaacat ttaaaaactt ccctgtggat ttctacctct 223200 tacctaagct ttgttcttgt ttttttaaac ctcatgaaca cgaagcttta gatagcggag 223260 gtcatgtatc cttgggctca aatatacagt ttcttagcaa tctttgattg ctacattgcc 223320 cttttttgtg tttttaactg gccttccctg acttaaaata tactttaaaa aataacaaag 223380 gtggtattta gtttgcacag tatggaatta aaggtaaaca actttaaatt aaatttggtt 223440 ggatgtataa attattaaaa tatcctttct ctcatggctg ggtaagtctg cattttccat 223500 tcagcctgct atgtctcttt ccatgagaac actcccgaag aatggggccc tgcttccaaa 223560 caagccaagc attttgtagt aaattttaaa aagctaaatt tcataataac ctttataatt 223620 gctacctgaa aatggagttt ggaatggcac caaattatta atcagtagaa ggagaataga 223680 ttgcaaaaag gtaattatat caaattgaat gcttcttgaa atatattctg ttaaataaaa 223740 tacagttcct cttccctgtt ctcatttgta agcaatgagg acaattcagg tagcatgaat 223800 caggaatcag agtacttttc tgttccgcag attggcttaa tcattttggg ctcacttcaa 223860 actgtagatg agcattgtcc tttaacctgc cgggcagcag aggtgaccac gttcactgca 223920 aagcccagaa gtgagttgtt catctgggtt agggtctcag gaagaggcat gttatgactt 223980 ccccacattc ctggacagtt tagactgtaa ttcttggttg tttaaaaagg tgtttgaatg 224040 tattttgttc agaggcttga gtaaatggct cttattctta cacttttttt tttgcctgct 224100 ttttccagca ttagaatagt ttcagccctg acaattagcc ttatgctgac tggagtggat 224160 tttttgcgta ggaccaagtg ttttgcatga tttacaatgg actgcataaa tgtagcctaa 224220 caagttatat gatgtgaaga aaattactta agggtaaaaa tggattgttt actgagaaaa 224280 tgatgttatt ataaactggt ttgagggtat tgtggtttag atgctgtcct tacaaatttg 224340 agctctgttg gtttggagat ttaatatcta ataataatag ttaatgtgta atgaatactt 224400 gccctgctcc aggctctatg ttacgtgtcc tgaatgcatc atctcattta atcctccata 224460 aaatttgaag cagtcagcat tgtaattaat ctcacttttc agatgaggaa accaaggtat 224520 gtaagaaaca aaaccagtgt tgaatctgac tagtacgact gcaaagcctg actcttaacc 224580 attgtgttct gttgccttct ccaagggaaa ggagtgcttc cagaaaggcc attggaagtg 224640 aggagtgtca cctgataata gctggctcct catcccacta ggttaacagg caacagatgg 224700 gggccacaga gttggctgga gttagtcctg gttgccaagg gtaaatgggg ctgttaccat 224760 gcaatggata gaaactagga tgctcctgtg tgtctcctta tatcctacca ggtgtagcat 224820 gaaggttaag gacaattccg tcctcttatt tggagaggag accaaggccc tgtaggacta 224880 aaggtttggg tgagctgaaa gccaagggca attggcccag cttggggagg agagttacaa 224940 atacatttgt gactatcaga ccagaaacag actgcggtgt tcacctgcgg tttgggtacc 225000 tgctgcttgt tctttgctgg ggtatctgaa gactgaaaac acagctcaca cttagccttt 225060 ttcctattct gtgtgaaaga actgtagtag ttttctcttg cttaacaagg ttctagtggt 225120 gaagtttatt gcttagtctg tgagttgtag ggaaataacc acaggatggg atggtagcag 225180 aacgagaaaa gaaaagaaga ttgtagatgc caggctggtg tcggcttgaa atttttaaag 225240 tatacagttc ttctttatgg attattaaaa aaaaacagca aatgaataca caagattaaa 225300 tgttaagcag tacaacattg tacatgatga aaggttaaag tctttctctg gtctcattca 225360 aaaatctaca gtctcttgta gtatagtttg aagtcaggta gcgtggtgcc tccagctttg 225420 ttcttttggc ttaggattga cttggcaatg cgggctcttt tttggttcca tatgaacttt 225480 aaaattctgt gatgaaagtc attggtagct tgatggggat ggcattgaat ctataaatta 225540 ccttgggcag tatggccatt ttcacgatat tgattcttcc tatccatgag cgtggaatgt 225600 tcttccattt gtctgtgtcc tcttttattt cattgagcag tggttttgtg gttctccttg 225660 aagaggtcct tcacatccct tgtaaggtgg attcctaggt attttattct cattgaagca 225720 attgtgaatg ggacttcact catgatttgg ctctctgttt gtctgttatt ggtgtataga 225780 atgcttatga tttttgcaca ttgattttgt atcctgagac tttgctgaag ttgcttatca 225840 gcttaaggag attttgggct gagacgatgg agttttctag atatacaatc atgtcatctg 225900 caaacaggga caatttgact tcctcttttg ctaattgaat actctttatt tctttctcct 225960 gcctaattgc cctgtccaga acttccaaca ctatgttgaa taggatggta ctggtaccaa 226020 aacagagata tagaccaatg gaacagaaca gagccctcag aaataatacc acacatctac 226080 aaccatctga tctttgacaa acgtgacaaa aacaagaaat ggggaaagga ttccctattt 226140 aataaatagt gctgggaaaa ctggctagcc atatgtagaa agctgaaact ggatcacttc 226200 cttacacctt atacaaaaat taattcaaga tggattaaag acttaaatgt tagaactaaa 226260 accataaaaa ccctagaaga aaacctaggc aataccattc aggacatagg catgggcaag 226320 gacttcatgt ctaaaacacc aaaagcaatg gcaacaaaag ccaaaattga caaatgggat 226380 ctaattaaac taaagagctt ctgttctttg ctggggtatc tgaagactga aaacacagca 226440 aaagaaacta ccatcagact gaacaggcaa cctacagaat gggagaaaat ttttgcaatc 226500 tactcatctg acaaagggct aatatccaga atctacaaag aactcaaaca aatttacaag 226560 aaaaaaggaa ccccatcaac aagtgggtga aggatatgaa cagacacttc tcaaaagaag 226620 acatttatgc agccaacaga cacatgaaaa aatgctcatc atcattggcc atcagagaaa 226680 tgcaaatcaa aaccacaatg agataccatc tcacaccagt tagaatggtg atcattagaa 226740 agtcaggaaa cgacaggtgc tggagaggat gtggagaaat aggaacactt ttacactgtt 226800 ggtgggactg taaactggtt caaccattgt ggaagacagt gtggcgattc ctcagggatc 226860 tacaactaga aataccattt gacccagcca tcccattact gggtatatac ccaaaggatt 226920 ataaatcatg ctgctataaa gacacatgca cacgtatgtt tattgcggca ctattcacaa 226980 tagcaaagac ttggaaccaa cctaaatatc caacaacaat aggctagatt aagaaaatgt 227040 ggcacatata caccatggaa tactatgcag ccataaaaaa ggatgagttc atatactttg 227100 tagggacatg gatgaagctg gaaaccatca ttctcagcaa actattgcaa ggacaaaaaa 227160 ccaaacactg catgttctca ctcataggtg ggaattgaac aataagaaca cttggacaca 227220 gggtggggaa cattacacac tggggcctgt tgtggggtgg ggggaggggg gagggatagc 227280 attaggagat ataactaatg taaatgatga gttaatgggt gcagcacacc aacatggcac 227340 atgtatacat atgtaacaaa cctgcacatt gtgcacatgt accctagaac ttaaagtata 227400 ataaaaaata tttaaaaaat ctacagtccc tttctcaata ggtaaacact attaacaatt 227460 tcttctgaaa attacatatg tacacacaca cacaccccac acatagatat tttgttaaca 227520 tacattacct tatgctctac acattattct gtgcctagct tttctcatcg aataatgtgt 227580 attggagatc tttccatata agtacatatt ggtctcactc attcattttt aatggctgta 227640 tattagtcca tagtatggag taataatacc ttttattggc acaaagtttt gcagttgaca 227700 aagggtttgc atatattgtt tcattgggtt gtatagcagt catcaacgga catatcaaga 227760 atcccagaag ggtaacctgg gcttcaccca ggatcgcatg gagcttggga ctcaaacagc 227820 taacattccc taaacaggcc catattcctg caatttagtg cagctgctac aacatttagg 227880 taaactcttt gaaagcagta aaaagatcac caatctggga catcaagaag ccagacgaca 227940 aggagacata gacctcaggt gagtgagaaa agagaataat tgaagtttag aactgaatgg 228000 gcactaacga acaacagata catctctttc ctttcaccga tgaggacctg ggtgtggcct 228060 gtggatgtta agtagcatgt ttaggccaca cagctggatt gttttctttc ctgaggctag 228120 ctctggagag gggaggaaac ggagatgcta gatttttccg ggctctcttt tctcatcagc 228180 tagaatggga tgtggatgga atgcagcttg agaagccaaa cacccttgag aaatgagaac 228240 tctgctttct gatgtggggc gcatcctatc tgagataact ctcctgccag cgcagtatga 228300 ccattgcgac ttgagctgtg tgggtgccca ggtggtgtag accttaaaac cactcctgct 228360 tttgtttact gaatgtctaa tattcacaca gttctttgtc tgagtgcagg gccaaagcag 228420 caaataacga agaagctgtg cccaaccata agaactatga aatgctccag atcatctacg 228480 ctagtttgag ttaaatttac catgcaaata aatatagaac atttcttctt atgcatagat 228540 tgtatttgtt ctattaaaca accatcctgt aaaggtttta ttcattcatt tatttttatt 228600 tgttaggaga aattgttatt taagctaaga aagtagacta gtagttattt cttttttttt 228660 gcttattata ctttacgttt ggggatacat gtgcaggtta gttacgtagg tatacacgtg 228720 ccatggtggt ttgctgcacc catcaacctg tcatctaaat taggtatttc ttctaatgct 228780 atCCCttCCC tagCCCCCCa CCCCCtgaCa ggccccagtg tgtgatgttc ccctccctgt 228840 gtccatgtat tctcattgtt cagctcccac ttatgagcga gaacatgcag tgtttggttt 228900 tctgttcctg tgttagtttg ctgagaatga tggtttccag cttcatccat gtccctataa 228960 aggacatgat tgcatccttt tttatggctg catagtattc catggtgtat atgtgccaca 229020 ttttctttat ccagtctatc attgatgggc atttgggttg gtttcaagtc tttgctattg 229080 tgaatagtgc tgcagtaaac atacgtgtgc gtgtgttttt agaatagaat gatttataat 229140 cctttgggta tatacccagt aatgggatgg ctgggtcaaa tactagaagg ctacagtaac 229200 caaaacagca tggtactggt accaaaacag atatctagac cactggaaca gatcagaggc 229260 ctcagaaata atgctacaca tgtacaaccc tctgatcttt gataaacctg acaaaacaag 229320 caatggggaa aggattccct atttaataaa tggtgttggg aaaactgact agccatatgc 229380 agaaaactga aactggaccc cttccttaca ccttataaga aaattaactc aagatggatt 229440 aaagacttaa atgtaagacc taaaaccata aaaaccctag aagaaaacct aggcaataca 229500 attcaggaca taggcatggg caaagagttc atgactaaaa taccaaaagc aatggcaaca 229560 aaagctataa ttgacaaatg ggatctaatt aaactaaaga acaactgcac agcaaaagaa 229620 actatcatca gagtgaacag gcaacctaca gaatgggaga aaatttttgc aatctatcca 229680 tctaacaaag ggctaatatc cagaatgtag aaggaacttc aacaaattta cacacacaca 229740 cacacacaca cacacacaaa caaccccatc aaaaagtggg tgaaggatat gaacagacag 229800 ttctcaaaag aagacattta cactgccaac aacataaata tgctgccaac aagcatatga 229860 aaaaaagctc atcatcactg gtcaagagaa atgcaaatca aaaccacaat gacatactat 229920 ctcacaccag ttagaatggc aatcattaag aagtcaggaa acaacagatg ctagagagga 229980 tgtggggaaa taggaacgtt tttacactgt tggtgggagt gtaaattagt tcaaccattg 230040 tggaagacag tgtggcgatt cctcaaggac ctacaattag tagttatttc taggtctgac 230100 gggctgtctt ttagtttgtt ttcttattct gaggtggact taaaccaatt ttaaaaacag 230160 ggatgactga attcgtgtgt taatcttgta ctacaggaac cctttgacat tgattcagaa 230220 gcacccaagt gtttgttcat taatcctttt ttatttttgc atattattta tttttcaact 230280 tttattttaa aaatcagggg tacatatgca ggtttgttac aaaggtatat tgagtgatgc 230340 tgaggtctgg catatggatg aatctgtcac tcaggtaatg agcatagtac ccaatgatag 230400 ttttttgtac ccaatgatag 'ttttttgttt tctttgtttt tttttgtttg tttgtttttt 230460 gagatggagt ctcgctctgt cacccaggct agagtgcaat ggcttgatct cggctcaatg 230520 caacctccgc ctcccagatt ccagcaattc tcctgcctca gcctcccaag tagctgggac 230580 tacaggcgta caccaccatg cccggctaat ttttgtattt ttagtagaga tggggtttta 230640 ccatattggc caggctggtt ttgaactcct gacctcaggt gatctgccca cctcggcctc 230700 ccaaagtggt gggattacag gcgtcagcca ctgtgcctgg ccccaataga tagtttttca 230760 gtccttgccc ctgttcctcc ttcccacttc tagttatcac cagtgtctat tgtttctgtc 230820 tttatgttca tgtgtaccaa atatttagct cccacttata agtgagaaca catggtattt 230880 ggttttctgt ttctatgtta gtttgcttag atagacctcc agctgcatcc acgttgctgc 230940 aaatgacacg atttcattct tttttatggc tgtgtagtat tccgtggtgt agatatacca 231000 tatattcttt atctaggata actgatgggc aattgggttg attccatgtc tttgctattt 231060 tgagtagtgt tctgatgacc atatgggtgc atgtgtcttt tcaatagaac aatttatttt 231120 cattactctt atcaggatac ctgggaggaa cttctcctac gaagttaatt tggggggact 231180 cctgaagatg agtgaaaccc cttattaagc acttagaggg tcgaaagtgt acaagggaac 231240 gttcaggtgt gacagcttga gagacatgca tattccaccc ccacaccaga gcttagtgaa 231300 ccgtcttttc tattttctcc caaagcacca aaatggccca gaaattggaa gatggaaaat 231360 ggacttattt atgagtctgg gaaggcaggg cagtaagcac agtcctgttc agggttctga 231420 gttttaccct cttgctctga ttgcgagatc attttcctct tccttgcttc ttcagcttag 231480 gacaagaaca gtttggaaat tgtttctact actttggaca ccatagttta gatttcaaat 231540 gagtacaagt gggaggaaag cttggataat tctctggaaa taatcgaaca gagtgaagga 231600 gaggggtcta caggtgagct gaatgctgca tggcattgaa gtaatcacac cagtcgtcac 231660 aattctctcc tctttgatgg ttatctgttg tcccagaagg aacaatttca gaaagtcctt 231720 ttctggactg tagaatagca cttgcttatt tgatgagccc tgagaagcat tactgaaagc 231780 ggttcattgt ccctgaggta ttacaatgag atggtggtca ctgatttcat tatgttttcc 231840 tttattgcag ctgttggttt gatcctttgc caggtgctta aaacaattgt ggttttgcag 231900 atggtaagtt agaggttgga caaaaaaagg gatcatgtca ctgccctggc caaaatttca 231960 acagactggg gtctagtgag ggcaaataga tagaggcttt. cctcttcact ttgtgttatt 232020 tagaaaaaga aactttccag gacaaatttc tttcctagaa ttcctttttt aaaaattttt 232080 tttctttgaa aatttactta gatgcaaata atatattttt cttcctttta aataataaaa 232140 gtaagatgtc tcttggaggt ggtggttgtc actgacaaga ttaactagaa ctgactagct 232200 gtaaaaatat aatttgggat gcattattaa ggcatgccat ttttatttgc atgccattgt 232260 gtacagatgt ggttgtgaaa tagttcaaat catggcacat tgaatgtcct cactggattt 232320 ttaggaatgt gttcactgag acagccaaat cctatttcat tttctttggc tcattgcatt 232380 ggctgtaaat tggagatatt cactttaata tgtgagtcaa aatttatttc caaacataat 232440 actgcagttg ttctgtcaca gaatataaat ttcttattta tttccttaat acgttgcttt 232500 ctactttttc tttttttctt tattttattc tggagtatgt ggaaaggttt tccagaaaga 232560 tttgcatatg ccataatcta ctgatgaata ctttttttgg gttactcttt catattttgg 232620 gagatataac tatggaagtg ttaggaatca tgggttctgg aaatagtttt attactgctt 232680 ctgaaatgcc ctcccaatga taccatatag taattccatc agggaataat atttttatta 232740 tagtttaaaa tataacttaa tatttaggtg ctcttgttca gtcatcgtca agttcttttt 232800 atttcaccaa ccctaccatg gcactcctga aagacttgtg aatgcgacag acctggattt 232860 aatcatggct ttgccatctg ctagccaaga gaacttgaac aagtgagtca acttcttgga 232920 gtctcatttt ctgcttctgt aacatgggaa ctagggtaat ctaactcatt gcttgtgatg 232980 attagatgag gcaaaatgct gagttcacct agcccagcac ctggtccatg ggaagcatgt 233040 gggttctgct gctacccagt ccttggccca gtgcatggtg cacagaaggg aatctgaaca 233100 ggccaacttt attcctattc ttgacccacc ccatgtagat gcttcctaca tcttcagctt 233160 cttcttcttc ttcttt.tttt ttttaaggca gggtctcact ctgtccccca ggctgaagtg 233220 cagtggcaca accacagctc agggcaacct cgacctccta ggatcaagtg atcctcccac 233280 ctcagcctcc tgagtaactg ggatgacagg accacactac cacacttggc taatttaaaa 233340 acttttgtag agctggggtc ttgctatgtt gcccaggctg gtctcaaact cctggattca 233400 agtgatgccc tcacctcagc ctcccaaagt gcctggaaaa caggcctcca cacccagcct 233460 tcaacttcat tttaaaaaat tgtggt,aaac tatacaattc atccatgaaa gcagaaacca 233520 cttgtacccc aaaagctatt gaaatttaaa aatatatata ttaaaaataa aaataaaatt 233580 gtgataagat atacataata tgaaatttac tactttaatc atttttaagt gtacggttca 233640 gtggcattga gtacattcac atttttgtgc aaccggaact tttcatcctc ccaaagtgaa 233700 gctctgtact tattttttat tttattttat tttttgagat ggagtttcac tctagtcgcc 233760 caggctggag tgcagtggtg caatctcggc tcactgcaac ctctgcttcc tgggttcaag 233820 agattctcct gcctcagcct cccaagtagc tgggattaca ggtgcccacc accacaccca 233880 gctaagtttt tgtattttta gtagagacgg ggttttgcta tgttgggcag gctggtctct 233940 aactcctgat ctcaggtgat ctgcccgcct cagcctccca aaatgctggg attacaggca 234000 tgagccactg tgcccggcca gctctgtgct cattaaacaa tgactccaag ttccccttcc 234060 ccacatctcc tgctgacctc tcttctactt tctgtctctg tgagtttaac tattctaggt 234120 acgtcatgta agtgcatcta tgtgatattt gtccttttgt gtctggctta tttcacttag 234180 cataatgtct tcatgattca ttcatgttgt agcatgtgtc agagtttcct tcctttttaa 234240 ggctgaataa tactccactg tatggataga ccacacttta tttatccatt tgtctgttga 234300 tggacatttg gatgatttcc atcttgtggg tatactgagt aattttgcta tgaacatggg 234360 tataaaaata tctatttgag tttctgcttt caattatttt gggtctacac ctcaaagcgg 234420 aattgttgga agtggaattg ctttccactt gttggaagtg gaacatggtc tcccacttca 234480 ttttgacaca acttccaagc ttcagaactg tatttacaac agcgtgctgg gaggtcgctt 234540 tgagtttatg acggaaatct catatcaaca ggttttaaat tgttccatac gacttcttgc 234600 cacccctgtc ccaattcaga tctttttttt gattaatggt atgaacaatt ttcaaaatat 234660 cctggcacaa tctattagaa tatttgactt tctcctcctc ctccctttag ccctaactat 234720 ttggcaaagc tgttgaaaac ttcttcacat cttctctctg cctcatctac tttgctactc 234780 aaagatg.att agtagatgtt ttggtatgat tttttttcct tggcataact gtgaatactt 234840 ttaatccttc atgttttatg acaatcatac attcccactt ttgtgtaaaa acattttgaa 234900 ttcttctttc tataacaaat tctttgccag tcttcctttt ctttttgctt ttagtgagtt 234960 ccaatgatat cattgtttga acctgttttc taattctatg ctgctattcc catactccct 235020 ctttgctttg gttacagtag ataatgtggg tggtcctacc aagacaaagc atcactcagg 235080 agtcaagggc tggggtagat acagacacta ggtactgcag aaagtcaata tctttctcag 235140 agtgtaaggc aggacaagac ttctgttccc ttgtaccgtt gtggctgggg agttggactg 235200 tgcatttcat ccttgtcata taagtcaaag tattgcccaa ataacttaat tttggtgtgg 235260 tcttagtaag tatttgtctc gtagatatgt taaatagaag acagtaacat tgggttggct 235320 gtgttaattc ctcacttttt ctttcctata catgagcttc ctaagaagcg gaacacttgg 235380 tggtcagaca gttgcagaga ttccttgcat gattgcaatc tggaaagtag ataccattct 235440 tgaatgaaga gcttgccctt tggagaagct gggctttcca tatatggagg ttgttagaat 235500 gcataagagt tcagcttctg gaatgaaagg agaccttggt tcaaaaccca tctgccactt 235560 gcttgctgtg tggcttaaat caggctttta aacttcaatt ataattttgc atctgtataa 235620 taggattaat ataattcctt cctcataaag ttcctagggg gttaaatgaa acaatcaatg 235680 tataacacac ttcctggcat gtgatgttca gtacctagaa gactcggttt ccttggtaga 235740 gagaatattt ggctagacaa gcttatggaa ttacccccag ataggaagtg agcacaagtg 235800 tgaaatgaac aagccagagc aggaacggct cttggaagcg tctactcagg cctgggcagt 235860 tgctggttta tataacagtg ctttgaaaat tcacgtgcag attcttacta ttttcccaaa 235920 tgttacagct caaaactatg ttgtctgtac cttaacacct aaaggataat atagtctttc 235980 actgataaaa ctaaaatgtc ataggttttc ctttggccaa atatgtatag aaacttgtga 236040 tttcacatca gatttaaagc tgtatttaac actctatgaa aacatactga tgcttagaag 236100 tagaaaggaa gtcagatttt gacatcttac ttgtcaactt aaattattta tagttcctgg 236160 atgcttcaaa atgtgataaa ccatagttaa ttttatgtaa atattcgatg agtgccttta 236220 ataaggagac tgtaaaggta gccaagcttt atatatgtta gctacattta tgggtcaatc 236280 gggtataaaa aataggactt cgaaaataaa atattatttt gtcggactcc tccaatgagg 236340 ctttttcgca ggattagcta aaattggctc ttatttgatg tgtgagtgct taaacattgg 236400 agaattcatt tttcttttag aattcatttt tatttctgag ccttaaaata tgaacagtta 236460 gctaaatgtt tgtatatgtt gataaggaat gctaagtgtt tattccttaa tgggacgaca 236520 ccttttcccg gtttacataa cttgcctttt aatctaacct tatgaaagtc tccttgactt 236580 taattttttt tcagagtact gtatatctct ttagggaatg catttattta aaaaattata 236640 aagcaagaat agatgtgata tattttgaag ttttctagtc acaaattaaa tccctagatg 236700 tgttgtagtt tgtggagcac tttgaaatgt gccaattcaa gatggaaata gcaggaaaga 236760 accattcaag tacgatttct gactccataa agttaggaag ttatgataaa ggaaaaataa 236820 ctacaccaca tacttatggc acagaattgc attattggga caaattgatc ttcaaatttg 236880 taggctatga tggaagcaaa tatttgtagt atcttaatat tcaactgtta agccaggaga 236940 cgagtactct gaacttcagc ttctcataaa atcagtcagt tacatgacag tttatgtagt 237000 ttatatgtaa gaaacccttt gatcaagata tgccttttct tcagccttgt taatacttca 237060 tttataagga tttttatttc taggaaaata ataccataga cctattttat ttaaagctaa 237120 agtgtttcct ggtgatggtg gtgaatgggg agatgattca aggaaactgc taatcttgta 237180 gagtttagta aaatcttgga atagaaattt taaaaagtta aaacacacta tgaaaacaaa 237240 tcattattag taaaatgaac catattaaaa tgtctccata accaacgtat tatagcaggg 237300 gaaaatggca ttttaattca gaaaaacatt tctatataaa acaagctttg gaataatttg 237360 aatatgttga tttttctttg gggcaatcat gaaatacagt catattagga aagaggcaag 237420 gcctcaaaac ggaaagagta gtgaggataa ttcatgagca atgcgtggct tcatggatcc 237480 cttcctggcc ctcttcatct atgaacatct gctttatgtt catgtctggc ctcacccagt 237540 gctgaagagc agactgcccc tgcttagaac caagccttgt tcttgtggat ttgagttttg 237600 gggtcctgag gtagaatggc catcatattg ttcagggtcc tcaccttccc actcattact 237660 tcctttatag aaccccagtc atccccctca ggaggcctgc gctccacaaa tgaagttggg 237720 ggtgagggga gctttagcat ctcagtattg ttcagatatt ggatacttgt tgagctccac 237780 tatgtgtgtg agaatgcgct gggctcagga tcaatagcca taaatgagac agatatggcc 237840 catcccttgc catgcctaaa ctgatctggg cattgagaca agcagttaaa acccaacata 237900 gtaagtccta tgatgagaca caaactaagc atggctggaa gggcagataa gatttcacag 237960 aggaactgac atcttgactg gcacacttag aagatgagta ggagtcagtt gggccaagag 238020 gagaaaaagt gtgtttccat gaagaagaag agcatgtgcc aagtcctggg ggtgagaagc 238080 atggcatgtg tcaacagaaa gacatgatca gagcttggat gggagttaga gcggggagag 238140 aaggcaagga aaaacataag agctttgcaa actgtgaagg cattgcattt agatgtcatt 238200 tttagagctt ggaggagcct caggacattg aggcaggata gtggcctgat ggcgtatgat 238260 tttaggaaaa atcactgtgg ccaccctgtg gggaaggatt ggcgggggaa ggctggaggc 238320 aaagggtcct gcggtgggct gtggtaatga tctgggctgg agaaggtatg gtggtgccca 238380 gcgcaaaatt ggtttcgctt tgaaaaataa ttagaaaata tttgttttga tctgtgtgcc 238440 tgtggtggga ggtggggagg gagggtcaag tgaggaagga gattgaatct agaataatgc 238500 ccatgtttca ggcttcacgt gcagacattt cttggttcag ctgggaagtg aataggaaca 238560 gactggcgag gaaagaagac agtcgtcagt tctgtctggg gcacatccat cttgacgagt 238620 gtgagagagt caagtcttga tgga.cagggg caggtggaca ttcaggaaac tcaggaaaga 238680 gatttgatct gaagtgacca ccagaaaata ctgacggaaa agaggtgaca aaaggagaga 238740 cctgcaatat tatattggct ttctctcagg caactgcttg gcctgtcttt atattccttt 238800 tgaatctctg catatgtacg ggccattatt tattttcaca actaacaaat gcagactttc 238860 tgtgtaatga caacaaagcc aaaccagctg ctaccaaagg agggaaaatc agaagagaag 238920 gaaaaagaac aagggaagct tggggaaaag ctgaatgtgg gtccttctgt tgctgcaggg 238980 gctggggtgg gcccggtgat tcctactgag aggcgttttc tctccccgct tcctgtcttt 239040 ctggttccat ctcattcacc tcctgtcccc tccacttcct gccagtcaaa ccttagattc 239100 ctccagaggc tttttatttt tatcttttga tgggcaagaa aatagtgcgg attatttttc 239160 caaaccttca cctgaacatc acatcgtggc tttggcccta tgggcttggt tcatccgggc 239220 ctgcacagaa ggacttttcg ggccagtctg gtcacataca tcgagtcctg tcttttcagt 239280 taaaaaaaaa cacacacaca cacactgcta tgtttcacta agacaactgg tgtgagttgt 239340 tttttagaaa atcaactcta cttcagtaag attttctcaa gcattatctt gagaagacca 239400 gataataaaa tttaaaaaga atttcttctt ttttttttat tatactttaa gttttagggt 239460 acatgtgcac attgtgcagg ttagttacat atgtatacat gtgccatgct ggtgagctgc 239520 acccactaac tcgtcatcta gcattaggta tatctcccga tgctatctta atgcaactta 239580 aatcaattgc tttaataaca catattgacc aagttacact cattaaggaa aaaaaactac 239640 tttgttgttt ttcttcttct gacgtgagct gaagacttag aaatagttgt taatagtgtt 239700 tggttaataa gaatttgttt taattgcata tatcaaatat gtatttatta aacttttctt 239760 tttgtcagtt tatcaattct agctttgtca caaaaggttt gccgtatgaa catgattctg 239820 ttgtacatct atttccattt ttgttaagag acgaattcaa ttgtaaaaat ctagtacctt 239880 ttattcatta aacatgttag ttcaggaatt tcacttggtt ctacaaagat acatatctac 239940 agtggatggc cagtgcaaac atgagactca gccaactggt ctctgaccca attcagttct 240000 cctgtcttct tctggcttac aaagtaactg gctctgggga gaaagtgagt caaagtaata 240060 tttggtttga atggttattg actattttct tctgaaactt aatgtatact aattaatttt 240120 ttattttatt cttttttttt tagagatagg gtctcgctct gttgtccatg ctggagtgca 240180 gtggtgctat cacagctcac tgcagccttg acctcctggg ctcaagcgat tctcctgtct 240240 cagcctcccg agtagctagg attacaggca tgtgccatca tgccaggcta atatttaatt 240300 tttttttttt gtagagacaa gatattgtta tgttgcccag gctggtctaa aactcctggt 240360 ctcaaactat cctcccacct cagcttcctg aagtactgga attataggca tgagctgcca 240420 cacctggcca tatactcatt ttttgttaaa agctgaaata tatcagcata tactgcataa 240480 ataccacagg agactaaaca ctgaaagttt ctttagggta tcagaagaat acactttttg 240540 cttgcagtta gcatctgcac agataagttt tgtttctggt tctattactt cttcagtttg 240600 accctattaa taaggacaat tctaaaaata ataactgtgt ctggatatct gaatccgtgt 240660 gtggtctttt actgaagtta caggtttata actctgctga ctagtttgct ggtttctgtt 240720 atgcaataga agagtgcaaa tgttaatttg atctgaagcc cgtgtataca gtgactttta 240780 taatgtatat ttaaagatgg aaagccaagt tttatgaggc cagcatttct tgtcaagtcc 240840 tcactccacc ctcttctaat tgggcctgac ccttaagttg aataaagaac aaagagctct 240900 gtagttaaaa catttcactg catgttgcat cttgccctta gtaaatggaa aaaaatagag 240960 acttaagcag agaatctgaa ctagggtgtg aaatatatat tcagttttgg ggtgggagaa 241020 tgagaaccat gttttacaat agtatacata acttccttag tctaaattca ggacatttcc 241080 ccaagatatg taagaattta gacttatgca gacagacttt ataaaaacat gccaatattt 241140 attagtttgt gaattttaat attctgctcc ctataaacca gatttatttt gagggataaa 241200 gggatggagg tgacttctaa atcttagagc agaaacttcc tgtgggcagc tggacatatg 241260 taccaggagc tcagagaaga gggttgtgtt gggagcgtac attctgagtg atctgcattt 241320 ggtgatgatg gaagccatgg acatgcacta gattgtcttg tgggagaata tggagtagta 241380 agagaagaag aagacctagg attgagccct gagcacctct ggcttaatgt tggatggagg 241440 aaattgaatc tgtaaacaat accgggaggc tgcagcctga gaggcagaag gaactggggt 241500 gtttgggatt atggaagcca agggaaaaag cctgtctcac agcgggaagg gaggtatcaa 241560 cattgtaagc tgcttcagat aggttatgta ggatgtggac tgaaaaatac ctgtaaaatt 241620 tggcaacgcg attcattggt aatcctaggg aagttgcttt tttggggtaa ctaaggtgga 241680 aaacagattg gtgtgggttg agcaatgcaa gagaggtgaa gaaaaggaga tttcatgtgc 241740 agacgtttct gagttcagct gggaacaagg gataagatag aagatggaag ttagagagca 241800 tgtggggcaa gggagactct tttatgggac agtctcgcat gtgatcaaag ccaatgagta 241860 aggagcattt gagggagaga gagtcaatca acaagagaga aaagaaagag ggttcatcat 241920 aaaataacgg taacaacaac gaacatcttt tgagtgtttt ctatatccgg ggaactatgg 241980 taaactccta acctgcattc tcacattcaa ttcttagaat cgttggatgt ggtgggttcc 242040 atgatttcct ctagattagc gaggaggaaa gagagatcta gaaatgtcag gtagcttgct 242100 cagagttctc caggtagtca gtcatggact aatttgtgaa ctgaaggact gaacttcgtc 242160 accacccagc ccaccaagcc ggcttgactt taggtattct gtgctgcatg tgagtaccga 242220 cttaaattat attttaagaa gggctacttt gaaactctct ctctgaaaac tctatttcta 242280 aaagctctac cctcacaaca attttggcaa gcagtcttgg taaaaccaaa ccaaaccaaa 242340 ccaaaaccaa aaaaccttat ctgctgagaa aatataacca cataaaatat ggtgctacaa 242400 aatatagact gtgtgaactg aaggtgactt gcccaaagga ctcctgaagc aattggctgc 242460 tgtagaaatt aagtccacgg gaggtttttt gttctgtttt tttttttttt tttttgagat 242520 ggattctcac tctgttgcct aggctggagt gcagtggcgc aatctcggct cactgcaacc 242580 tccgcctccc gggttcaagc gattctcctg cctcagcttc ctgagtagct gggattacag 242640 gtgtacacca ccatacccag gttttttttg tatttttagt agagacgggg tttcaccatg 242700 ttggtcaggc tggtcttgaa ctcctgacgt cgtgatccac ctgcctcggc ctcccaaagt 242760 tctgggatta caggcatgag ccaccgtgcc cggcccatga gaggttttgt ttgcacttca 242820 agaaggacag aaaaaggcag gcaggctggg gagcaacata gtaaggctga ggaagtgata 242880 ggaaaacagc ctccaaaagg tttccctgta gattctgact ggctaagttt cctgaaataa 242940 tattaattct gtcctcttgc ttttaatagg acataacgac tatatgtgtc cagccaccaa 243000 ccagtgcacc attgataaaa acaggaggaa gagctgccag gcctgccggc tccgtaaatg 243060 ctacgaagtg ggaatgatga aaggtggtag gtacatctct cccaggggcc cttggggatg 243120 gccctggcca ccgcccagtg ctggctctac ccattggaat aacaccatgg gaattttgtg 243180 tttttttctt ttaattgttt tttttctatt cttatttttc tttgcaacaa aagtattttc 243240 ataatccatt ttattttaaa aaggtggaag tgtctggaac tggaaattct aacatggcat 243300 tttgtgtttt ggattttcaa tgtaaataat tatattttaa atcaaaggtg tgtgggaggc 243360 ggtgatggaa ggaaacgaag agtgcttagt aaattattct agaaatattt ttcagttact 243420 gtttatgttg caaatgctag aaaa.tgatat ctgaggataa actttcccta aattgagact 243480 tgtaaatgtg aaagctgagt agctaattta tagccttcca gtctgttatc atcccttaag 243540 gaatgtgaat ttcaatcaaa aggcagtttt ctcctttaga acctgagtga accagccact 243600 ttcttaacct cagtgtctag catggtgctg gcatagttga ttactgagca ctacactaac 243660 aagtgtcaga gcatgcatgg tttgtgactg tgggtttgtg tttttgtggt ctttgtggct 243720 gtgtgtgtgt gtggttttct gtcttctcat gtatccgatc ttccagtttt gtcatacagg 243780 aatctggaaa ctgaatccca gttctgggaa tattaggagc cccataaatg tggttgcctg 243840 attgacctca ttgtattctt gggagtctca tcttgaggaa cattgcttct agtcttggat 243900 agccttcagt gtagtggaag agaacaagta gaaaatgtgt aattaaaata aagtcatgag 243960 gctgaggtgg gcagatcatc tgaggtcagg agttcgagac cagcttggcc aacaaggtga 244020 aaccctgtct ctattgaaaa tacaaaaatt agctgggtat ggtggcgagt gccagtaggc 244080 ccagctactt gggaggtgga ggcaggagaa tcacttgaac ctggcaggtg gagggggcag 244140 tgaactgaga tggcgccact gcactccagc ctgggtgata gagtgagact ctgtctcaat 244200 aaataaataa ataaataaat aaataaataa ataaataaat aaataaagta acggattcat 244260 ttagtgtttc agaaggatac tgaagggagg gaagggctga tgatggtgct acctgctgat 244320 tgtaataggg aaaagcccgt ttcttttctg aaggaggtga agcttggggt gatttaagga 244380 gaacaaaatt tcaatgaaaa ggaatagtga, ttttgcaaat agtgggcagc taacctttaa 244440 atcattctta ctgggacgca ggaggaagga gaggaaagac caagaatgga gattatgatg 244500 aagacaagct tgattgttaa caagcttaca gtgacaggtt tcatagtccc ggggtcataa 244560 atacgccaga caggttgagg tttggaatgc agcatttgga ataaattctc ctggtgaaga 244620 gatgcaatgt tgaatttacg cattctgtga gctgtgacta tgactataca tcttgaacat 244680 ctgaaaaagc agctttaatt caaagggtaa ttaattccaa gaatttaaca gctacattca 244740 gaaaatgaca cttgagtcat atggattaaa tatttaagga attcatcttt ctttgtactg 244800 ttggagaatt agtcaggctt aattaaactt acaaaatgcg tcttaaggta acttggtaag 244860 tgacaggaat ataacagctg cataagaaaa gctttatttg aaacagtgga gtcgaggttt 244920 aatattcttc tttggcgatt atatgtaggt taagcacagg ctcttccata cattctcctt 244980 gaaagctgaa ataatcaagt catgaatatg tccaaaacaa tttttaaaat gtgaggggta 245040 cccatgcatt gaccccattt cataaaaccg attctgtttt tttttttgta attaatatcc 245100 agatacggtc tggctgcatg aatataaatt acgcattctc atttttaatc taacaaaaat 245160 tcatatatgc aaagacataa taaaagtctc cccactgttt tctcctagga atctagataa 245220 tttgactgta cacaacagac tgtggatggc tccatacaca cttgcacatg tatattgatg 245280 actgtaaaat atatatctgt attagttttc tagggctgcc ataatgaaat accacaggct 245340 gggtggctta aacaacggaa attaattttc tcccaattct ggaagctgga catgcataat 245400 caaagtgctg gaatatttgg tttctagtga gccctccctt tttttttttt gagagatgga 245460 gtcttgctct gttggccagg atggagtgca gtggcactat ctcagctcac tgaaaccttc 245520 acctcccagg ttcaggcgat tctcctgctt caacttccca agtagctggg actacaggtg 245580 tgtgccacca tacccagcta atttttgtgt tttttttttt tgtagagacg gagtttcact 245640 ctatgttggc caggctggtc ttgaactctt gacctcaggt gatccgccca cctcggcttc 245700 ctaaagtgct gggattatag gcgtgagcca ccatgcccgg cctgtgaggc ctctcttcta 245760 ggcttgtggc tagccgtctt ctgcctgtgt cctcacatgg cctttcctct gcgtccgagc 245820 actcttggta tctctttctc ttctcacaag ccctgttgga ttaggagtcc acccttatga 245880 cctcatttaa ccttagttac cgccaaaaag gccctatttc caaatatagt cataccaggg 245940 gctagggctt caacatacaa gttttgtggg gacacagttc ataacaggct gccctcccaa 246000 aataccatag actgggtggc ttaagtaacg gaagtcaatt gcctcatagc tctggaggct 246060 gaagtctgag actaaggtgt tggcaggttt gatttttccc gaggcctctc tccttggctt 246120 gaagatggct gccttctctc tgtgtcccca catggccttt tctcggggca tccacagctt 246180 cttcttcttc ttacgaagac accagtcata tcggattagg gccccacaca caggacctca 246240 ttgaatctta atcacctcac ttaaggtaca atttccaaat acagtcacat tctgacjgccc 246300 agggggtaag atttcaacat atgagtttta agggtacaca attcagtatg tggcaatatc 246360 ccataggggt tactcagtga ttgttcagta tttctttaca tgtaaggaaa acatgtatct 246420 ttctggcagt ttttatatgt acataagttt taaatgaagc tttaaattca taaaattata 246480 gcccgtgaga acatcttatt ttaatgatat gtataaagta cttgccataa atcaatataa 246540 tttattgtat tttctacaac atggaatacc acacatggcc acaggatacc ctctataaca 246600 actccttgaa aaatcccatt gaataaagcc ttaatttaca actggaagta caatgataac 246660 taactataaa atgtgcagtc acatggcaaa ggccagctgt gactgaataa atctttacaa 246720 catgttttca accattaggg ttcacaacaa tccactttct taaccaggct ggttaccaac 246780 taggatattg aaactctacc tatgaaataa aaagtttagt ttcagttaaa agagaaccac 246840 agacctactc cctattttca tagcaaggct gaaaaaattc actgaagatc tattgtaaag 246900 atccaaaaga gtgcatgatt tgtctccatg aactaacaat tttaatctgt tataatactt 246960 actgcctctg aaaatcccag catcattatt agatttggtt ctgaatcatg gtagttctat 247020 aagatgcagg atttgaattg cactttcggg aatgtgcttt tctatcctag tacatgtgct 247080 ggtttctctg cattgctttt tcctcttcat ttgtctcctt cctcttgaac tttaggttgt 247140 agattacatg tctcttcttc aggaaggcct acacagttac ctgacagttc ctgaggctcg 247200 aagttcaaaa tcaaggtgct ggaaaattca tgttctagct ttaatggcaa acagctcttc 247260 tcttagctct tatcaccatt attattagtc attcaatgtc tgtctttaca tatggtccat 247320 aaactccatg atggcgcggg ggtggggggt ctctgtgtct.tgtttaccga tcttgcatga 247380 aacaagtcac aaggtaggtg ttcaagaagt atttttttgc actaattaat gaattgattc 247440 aattcattta aatagttatt tgtgtagggc ttcttatatg caaacccttg ggaataaagc 247500 aaatatgaat aagattgtag tcccttccct caatgtcctc acagtataaa gagaacattt 247560 gcatgggaag cggcaaataa aaatctcaac ggcaagtgga gactgggttg caagatggct 247620 caaatattga atgatcagaa tgggagtcta gagctttaaa caaccactcc caggtttagt 247680 ccaactcaga gtattactca gacacctgac atccaagaca tatattatta ctcaagaatg 247740 tggccttttt gctgagtttg gtagaaagag gaactcttcc aaagataaat gtgttcaaat 247800 gctggctcct cctcaaactt gctgaacaac cttgttaaag tcacttttat gagcttcact 247860 aatttcaaat atgaaatgga gataaataaa ttttattgtc caagcacggt ggcttacgcc 247920 tgtaatccca gcactttggg aggctgaggt gggcggatca cctgaggtca ggagttcaag 247980 accagcctgg ccaacaaggt gaaacctcgt ctctacaaaa ttagccaggt gtgatggcag 248040 aagcctgtaa tcccagctac tcaggaggag gaggcaggag aatcacttga acccagtagg 248100 cacaggttgc agtgagccga gattgcacca ctgcattcca gcctgggcaa cagagtgaga 248160 ctctgtctca aaaataaata tataaataaa caaataaata aattttactt aatgagtttg 248220 tcatgaagat gacaaatcgt aacatgggaa aatataaatc taaagtgcca cacaagtgtt 248280 agctattaca tatctttttt gtcttaaaaa taattactat cttagttaaa aatttcatca 248340 tatacgagca ctgctgggtt ttttttttca gctgaaagaa agagaatggc aatttcatca 248400 cttctacaag atataaattt ctaatttctt tgtggagaat aaaaacttgg cttaaacacc 248460 taagcttttt tttcttttct gatctcatat gctgaatata gctgaatgac aagtgagatg 248520 tgttatttat cattttatga tgccagagat ttacacgaat ttgtgaaagg ttttttctta 248580 ccatagattc agaggcgtgt ttgaggcaag gttttcattg tgcagtgagg aaattggcac 248640 atagaaatca attgatgtgc aggactcttg gactgactat tctggttatg tttccttata 248700 acacattaag aagaaatttg agataaagta gtaattgtca actagtcttt atttatttta 248760 ataacaaatg ttgcagtata atttttagaa atattccaaa ataacaaata aatccaaaaa 248820 ttaacgggat aattttaaag tgagaaaata acatactact aatatagaca gggaaatata 248880 catgtgtcca gaatgattca gaagcccaat gaaacactgg aaaataaaat attttcctgt 248940 tgcttgggtt taatatttat cactatgagt tatcgtaatt tgctttattg atggataaac 249000 tcccaacctg gggactgcga ttggaatcct ctttaatcct acattggcta tcctttatca 249060 aaagtaaaca accggctggc catggtggct cacacctgta atcccagcat tttgggaggc 249120 cgaggcgggt ggatcacctg agatcaggag tttgagacca gcctgaccaa catggtataa 249180 cctcgtctct actaaggata caaaaattag ctgggtgtag ttgtgcccac ctgtaatccc 249240 agctatttgg gagcctgagg caggagaatt gcttgaactc aggagacgga ggttgcagta 249300 ggccaaaatc acaccactgc actccagcct gggtgacgga atgagactcc atctcaaaaa 249360 aaaaaaaaaa agtatacaac caattattaa tgttcatgat ggtagaatca aattaataaa 249420 ttaaaaatgt agatgtgaat tacttttttt ggattgccga ataattgtgg aagatttgta 249480 gctgcatgtg aatccaagtt tcctcaaaag gtaggttggg gtgctcctat ttaggattga 249540 atttaagaaa aatgttaaag aaaagctagc tgctctttac caaagagaga ctggttgttt 249600 taagcagtaa tctaatctat atatttatga tattgaaact attattttat taattcatat 249660 aatagatatt tattaaaaac tccccatatt ataatttttt atttgcaaac aaatatactg 249720 aatcaagtaa gtgaatcaca tatggacctt aaagactaat gaatccaatg tttgacagta 249780 ttttatatca atcaagctct ggtttccaca agcagaagca gatcgtcatc tatgaaacag 249840 ataaaagttg atttggcata ccctctcttt attcattatg atgttcatga gaaaccttct 249900 tagaacccta gggctccaag gaacacagtt tgcaagccac tgatctaatc taactccttc 249960 atcctctagg tatgaaaatc aaggccctcc attacctatt agggatgaag gcaggtcagc 250020 ctctgtgact gtcagttcag ctggaggaag catgagtgta ggcagcacaa caatccctat 250080 aaggaccatg ttcttaagct ggtttttttt ttcctagtag tttttacagt ttcaggtcta 250140 acatttaagt ctttaatcca ggttgagttg atttttgtat acggcatgag ataagtgtcc 250200 aatttcattg ttcggtgtgt agatatccag tttctctaga aacatttatc aaagaaactg 250260 tcctttctcc attgtgtgct tttagcagct ttgtcaaaaa ttaattggct ctaaatgaat 250320 gaatttattt ctgggttctc tattctgttc cattggtcaa tacatgctgt tttaattact 250380 atagctttat agtatatttt gagtcaggta gtgttatgcc tctagctttg ttcttctagc 250440 tccagattgt tttggctatt cagggttttt ttgtggttcc atacaaattt ttggattgtt 250500 tttccacttt tgtaaaaaat gtcattgcaa ttttgatagg cattgcattg aatctgtaga 250560 ttgatttgtg tcatatggat atttttaaca atattaattc ttccagtcta tgtatggtat 250620 atctttccat ttatttgtgt cttcttcagt ttcttttatt gatgtctata gcttttagca 250680 tacaattctt tcacttcctt ggttaaattt attcccaaat attttgtttt atttttatag 250740 ctattataaa tgaaatttct tcttgatttc tttttttgga tagtttgctg ttagtgtata 250800 gaaacactac tgatttctgt atgttgattt tctcttctga aactttactg aatttgttta 250860 ttagttctaa tagttttttg tgtggagtct atacttacat gatctatgtg taagatcatg 250920 tcatcagcaa acacgggcaa tttaacttct tctttttcaa tttgcatgcc tttatttttt 250980 ttcttgcata attgctctgg caggaactct cagtactatg ttgaatagaa gtggtgagag 251040 tgggcatcct tgtcttgtta ttgatcttag gggaagactt ttcaaatttt caccattgag 251100 tatgatgtta gctgtaggct tgtcatatat ggcctttatt gtgttgaggt acactctttc 251160 tatatttaat ttgttgagag tttttgatat gaaaggattt tgaattttgt caaatgcatt 251220 ttttcctcta ttgagatgat tgtatggttt ttgtccttca ttctgttaat gtgtgtacca 251280 tagttataga tttgcatatg ttgaaccatc gttgcatccc tgggcaaatc ccactcgatc 251340 atggtgaatg attctttttc atgtcatgac aatcaatttt gctagtattt tgttgtggat 251400 ttttgcatct atattcatca gggatattgg catgacattt aattttcttg taataccctt 251460 gtctggcttt agtatcacag taatgctggc cttgtaaaat gagtttggaa gtattccctc 251520 ctcttcaatt tttgggaaga gtttgaggat tggtattagt tctttaaata tttggtagag 251580 ttcagcaata aagcccatta ggtcctgggc ttttcttcca tagaggactt tttattgctg 251640 attcagtctt ctttctagct attggtctgg tcagactttc tatttcttca tgattcagac 251700 ttgctaggtt tatgtgtcta agactttatc catttattcc aagttatcca agttgttggt 251760 gtgtaattca tcatagtact cttataattt tttgcatttc tgtgctatca gttgtaatgt 251820 ctcctctttt atttctgatt cagtttattt gtgtcttctc actttttttc ttagtctagt 251880 taaaggttaa tcaattttgt ttatcttttt aaaaacaaat tcgtagtttc actgatcttt 251940 tgttttgtgt ttttagtctc aattttattt atttttcctc cgatctttat tattttcttc 252000 cttctactaa cttggggctt agtttatttt tatttttcta gttatttgtg gtataacatt 252060 agattgtttg agatcttctt ctttgatgaa gacatttatt gctataaact tccctcttga 252120 aaatactctt gctgcatccc acaaattttg gtatgttgtg tgtccatttt catttgtttc 252180 aagatatttt tcaacttttc tttttatttc ttctttgacc cactggttgg ttctgagtat 252240 gttgttttat tttcaagtat ttgtgaattt tccaaaatta ttcttgttga tttatagttt 252300 catattattt atggttggaa aaaatacttg atatggtgtc aatgttctta aatttattaa 252360 gacttatttt gtgtcctaac atatgaacta tactgttttt tttctttttt tttttttgag 252420 acagagtctc actgtgttgc ccaggctgga gtgcactggc tcaatcttgg ttgactgcaa 252480 cctccgcctc ctgggttcaa gctattctca tacctcagcc tcccgagcag ctgggaatac 252540 aggagcacac cgccatgcct ggctaacttt ttgtattttt tagtagagat ggggtttcac 252600 catttgggtc aggctgctct caaactcctg acttcaaatg atctgcccac cttagcctcc 252660 caaagtgctg ggattacagg agtgagccac cgtgccaggc catgatctat actgttttat 252720 ctaacattca gtggataatg ttccatgtgt attt.gagaag aatgtgtgtt ctgttgctgt 252780 tggatgaaat attctgtatg tatctgttaa gtccatctgt gctaaagtat agtttaagtt 252840 tgaactttca ttattgattt tctgtccgaa taatctgtcc attgctaaaa gtggggtact 252900 gagttcccca atattattgt attacagtct atcttccctt tcatatcaat taatatttgt 252960 ttcattcatt taggtactcc aatgttgggt gcatatgtat gttcacctgt tatatcctct 253020 taatgaattg accctcttgt cattatttaa tggccttctt tgtcttattt tatagtttgt 253080 gacttaaagc ctattttatt tgatataaat atagctgtct ctgctttctt ttggcttcta 253140 ttttcataga atgccttttt tggtcccttc gccttcattc tctgtgtttt cttaaccatg 253200 cattgaatct cttctagaca tcatataaca gggtcttgtt tttttaaatc catttactca 253260 ctgtatctct ttcttcctat cttgctgtct tcctttgtga ttaggcgatt ttctatagtg 253320 gtatcctttg aattcttact ttttgccttt tgtatatttg ctacaggatt ttgctttgtg 253380 attaccataa ggcttacata aaagatctta tagttatacc aggctatgtt caactgttta 253440 taacataact ttgattaatt ttttaatact caatttattt gattgcttat ttagagataa 253500 ttttgattcc tatcagaaga aaactgaggt atctttatgt accttttcag gaatgaccac 253560 gtctcacctt accttgaatg ttacaagaaa tgctggcagt gactcaaggc caggcaaaaa 253620 tttcagaaag ggtttatctt gtttgtacaa ttttcttttg caaaaataaa tgattcttga 253680 tatgatgtaa ggactttttc cgtagataga acctataaaa tgcagaactt tggggcagta 253740 attatatgaa tgaattgcta ctttaaaatt ctgtaagtcc ccaggaattt ccatatgctt 253800 ttttttgttt gtttcagtgg ttgtgatcag gctaatgggt agtttttaga gctgaaaaga 253860 acttaaagat catctagtgc acctttttgt ttttcagttg gattgttctt .gtccaaatca 253920 ctaagctaag ttaactgctc agacaggaaa ccaagtctcc tgacccatgg tttaatgctc 253980 ctttcattgt tatgcgtctg cctggctgac ctgttagtaa cataaagtgt catgagagat 254040 agacatatgc atgagtaaga tattaatatc ccataaacca aaactgtgac ttaggaaatg 254100 ttacggtggg cacaggatat tactcctctt caacaaaaac atcaatcata tgttggctta 254160 gttgcacagc agaagtacca aaataaattt atatagacgg tgtcacagat actttaaaaa 254220 gacctactta agatttaatt acctaatatt atttaatatt taatataatt taaaaggggc 254280 atttttatct ttcagccaag gttttaattg tttttagata attctatttt tgaaagtctg 254340 attttgtttt aaaatgccca ttctggtata taaaaacatc attttgtcaa ataggagtcc 254400 cagctaaggg ccaagcgtgc aattaaactt gactctttac ctactaccta ggaaccttgg 254460 gcagatcaca tactttttct tcactcagtt tttacttctg aaaaatgcca gctgaagtat 254520 gtttcaaatc ttaaatttta tgttttatga tctaagttca gattaacaat ggaatggtta 254580 gaatccaaag ctaaaggtag tatatacaag tgtatattgg gggttgggag caactgccgc 254640 agctatcata tttacatttc aagctcaatt tagaacaact gcgggcaact gtaaaaaccg 254700 gtaggaatgg gcagagttat tgtcttcact agtcattgtg gaaagtgaat ctcttctgaa 254760 tggagagtca caaaaatgag tttgatgctt tcatgatagt gaaatgagaa gatggccata 254820 tattgctata aatcaccagc aacataaaag aaaaaattca aaaatcaagt ttgttgaggc 254880 aggactctga aaagataact aagctttttt tttttttttt ttctgttaga gatgtaataa 254940 tcatgactaa gactggaatc ccaagagctg gtgagacttt ctagtttctg ccttgtggaa 255000 agagaaagga agaggccaaa aaagataatg aattttggaa agttcctgat taaatatcgc 255060 ataggctagt ctggttatgt ctgcttgaaa acttatccag taggtataaa ccaacacact 255120 tgaggccagt ctttttggaa attacagaaa tatatgtcag ccttaagaga catcctatga 255180 tgaagagcag agggagttgt ttataaagaa atgttctcat tggctgggca tgatggctca 255240 cgcctgtaat cccagcactt tgggaggccg agctgggtgg atcacttgag atcagaagtt 255300 ccagagcagc ctggctaaca tggtgaaacc ccgtctctac taaaaatata aaaaaattag 255360 ccgggcatgg tggcgggcgc ctgtagtcgc agctactcgg gatactgagg caggagaatg 255420 gcgtgaacct gggaggcgga gcttgcagtg agccaagatc gtgccactgc actccagact 255480 gggcgacaga gcaagactct gtctcaaaaa aaaattagcc aggcatggcg gggagcgcct 255540 gtaatcccag ctactcagga ggctgaggca gggaaattgc ttgaacccag gaggtggagg 255600 ttacagtgag ttaagatggc accattgcac tccagcctgg gtaacagagc tagactccat 255660 ctcaaaaaaa gaaaagaaat gttctggtca tgcctggaga taatacatgg tttagtttat 255720 gcttgattca gcaaataaaa attggtgagg aacatacaga attatattta ggtgattata 255780 ttttttatat tttatatata aaatttatat tttttatatt ttaggtgatt atatttttaa 255840 acgcttgctt tacaagacta atcattatta cttttttaag ttttgtgata tattttattt 255900 aactaatata ccccaaatag tatttcaata cagaatcaac atttaaaaat tattgataca 255960 ttatgtatgt ttttttcctt cactaagact tccaaatcct ccaagtgttt cacacttaca 256020 tcttattttg gaccagccag attttagtgc tcatttgacc atgtgatctg tgcttaagga 256080 ggctgctgga gctgaggctg ggctgcagta ccaacataga agtctttatc tgtcaaacca 256140 ggagtttgag atccctgagg gatatgggaa ccataaagga tcttaagcag agaagtgact 256200 ttcttaattt tgaacctatc ctggtgttct catagattag aggagagaga tttaggagtg 256260 agggatcaag caagaaggtg gctccacatt gttttccata gtagttgtac tagtttacat 256320 tcccaccagc agtatagaaa tgttcgcttt tcactacatc cacaccaacg tctattattt 256380 tttgattttt tgaaaatggc cattcttgtg ggagtaaggt ggtattgcat tgtggttttg 256440 agttgcattt ccctgatctt tggtgatgtt gagtattttt tcatatgttt gttggccact 256500 ttgtatcttc ttttgagaac tgtctattca tgtccttagc ccactttttg atgggattgt 256560 ttgttttttt tcttgctaat ttgtttgagt tccttgtaga ttctggatct taatcctttg 256620 ttagatgtat agattatgtc tccttctctg tgggttgttt gtttactctg ctgctgctcc 256680 ttttgtgcaa aagctcttta gtttaactaa gtcccactta tttatctttg tttttgttgc 256740 atttgctttt gggtttttgg tcataaaatc cttgcctaag cccatgtcta gaagggtttt 256800 tctgacgtta acttctagaa ttttatggtt ccaggtctta gatttaagtc cttgatccat 256860 cttgagttga tttttgtgta aagtgagaga tgaagatcca gttgcattct cctacatgtg 25920 gtttggcaat tatcccagta ccatttgttg aatagggtgt cctttcccca ctttatgttt 256980 ttgttggctt tggcaaagag cagttggctg taagtatttg ggtttatttc tcggttctct 257040 actctgttcc tttggtctac atgcctattt ttataccagt accatgctgt ttcggtgact 257100 atggccttat agtatagttt gaaatcaggt aatgtgatgc ctccagattt gttctttttg 257160 cttagtctta ctttggctat gtgggctctt ttttggttcc atattaattt taggattttt 257220 tttctagttc tgtgaagaat gatggtggta ttttgatggg aattgcattg aagttgtaga 257280 ttgcttttgg cagtatgatc attttcacaa tattgattct actcatccat gagcatgaga 257340 tgtgtttcca tttgtttgtg tcatctatga tttatttctt tgagaggtgt tttgtagttt 257400 tctttgtaga ggtccttcac cttcttggtt agtttttgtt ttttttggtt tttttttttt 257460 tttgcaacta ttgtgaaaag gagtgagttc ttgatttgat tctcaccttg gttgctgctg 257520 ttggtgtata gcagagctac tgatttgtgt acattaattt tgtatcctga aactctgctg 257580 aattcattaa tcagttctag gagctttttg ggggagtctt tagggttttc tagttataaa 257640 atcatatcat cagcaaacag cgacagtttg aattcctctt tactaatttg gatgtccttt 257700 atttctttct cttgtctgct ccggctagga cttctggtac tgtgttgaat agtgagagtg 257760 ggcatctttg ttttgttcca gttctcagag gaaatgcttt caacttttcc ccattcagta 257820 ttatgttggc tgtgggttta tcatagatgc cttttattac agtgaggtac cttgtatacc 257880 gattttgctg aaggttttaa tcataaaggg atgctggatt ttgtcaaatg ctttttctgt 257940 atctattgcg attattgcga tggtcatgcg atttttgttt ttaattttgt tcaggtggtg 258000 tgtcacattt attgacttgc atatgttaaa ccatccctgc atccctggta tgaaacccac 258060 ttgatcattg tggattatct ttttgatatg ctgtttgatt tggttagcta gtatttcgtt 258120 aaggatattt ttttgagatg gagttttgct cttgttgccc aggctggagt gtagtggcac 258180 tgcaacttcc actttctggt ttccagcgat tctcctgcct cagctccctg agtcgctagg 258240 attacaggca cctgccacca cacccagcta atttttgtat ttttagtaga gatagggttt 258300 cgctatgttg gccaggcggg tcttgaactc ctgacctcgt gatccacctg ccttggcctc 258360 tcaaaggact ctcaaagtgt tgggattaca ggcgtgagcc acagcgcccg gccagatttt 258420 tgcatctatg ttcattagga atattggcct gtaggtttct ttttttgttt tgttctttct 258480 tggttttggt acaagggtga tactggcttc atcgaatgat ttagggagga ttccctcttt 258540 cttcatcttg tggaatagtg tcaataggat tggtaccaat tcttctttga gtgtctggta 258600 gaattcagct gtgaattcat ctggtcctgg actttttgtg ttgttggtaa tttttaaatt 258660 accatttcaa tcttgctgct tgttattggc ctgttcagga tttctaattc ttcctgactt 258720 aagctaggag gtttgtatct ttccaggaat ttacctatct cttctaggtt ttctagttta 258780 tgtgtgtaaa ggtgttcata gtagccttga atgatctttt gtatttctgt ggggttggtt 258840 gtaatatctc ctgtttgttt ctaattgagc ttatttggac cttctctctt cttttcttgg 258900 ttaatcttgc taatggtcta tcaatttcat ttatcttttc aaataaccag cttctttttg 258960 tttcatttat cttttgtatt tttttttcaa tttcctttag ctctgctctg atcttggtat 259020 tttctttctt ctgctggctt tgggtttcgt ttgtttttat ttctgtagtt ccttgatttg 259080 tgaccttaga ttgtctattt gtgctctttc agagtttttg agtaggcatt taaggctgtg 259140 aactttcctc ttcgcattgc ttttgctgta tcccagaggt ttggatggtt gtgtcactat 259200 tattgttcaa ttcgaagaat tttaaaattt tcatcttgat ttcattgttg acccaatgat 259260 cattcaggag caggttattt cacttccatg tatttgcatg ttttggaagg ttccttttgg 259320 agtcgatttc cagttttatt ccactgtgga ctgagagagt agttgacata gttttaattt 259380 tcttagtttt tttgacactt gtttgtggca tatcatatgg tctatcctag agaaagttgc 259440 atacgctgat gaatagaatg tatattctgt ggttgttggg tagaatgtta tgtaaagatc 259500 tgttaagtcc atttgttcca gggtacaatt taaatccatt gtttctttgt tgactttctg 259560 tcttgatgac ctgtctagta ttgtcagtgg agtattgaag tcccccacta ttattgtatt 259620 gctgtctgtc tcattactta ggtgtagtag taattgttct atacatttgg gagttccagt 259680 gttaggtgca tatatattta ggattatggt attttccttt tggacaaggc cttttatcat 259740 tatataatgc tcttctttgt cttttttaac tgctgttgtt ttaaagtttg ttttgtctga 259800 tataagaata gctactccta cttgcttttg gtgtccattt gcatggaatg tctttttcca 259860 cacccttacc ttaagtttat gtgagtcctt atgtgttagg tgagtttctt gaaggcagca 259920 gatacttggt tggtgattgc ttatccattc tgcaattctg tatcttttaa gtggagcatt 259980 taggctattt aaattcaatg ttagtattga gatgtgaggt actattctgt tcatcatgcc 260040 atttgttgcc tgtatacctt ggattttttt tagtagtatt tttgttttat acctccaatg 260100 agatttacac attaaggagg ttctgttttg atgtgtttcc agcatttgtt tcaagatttg 260160 gagctccttt tagcagttcg tgtagtcatg tagtgctggc ttggtagtgg cgaattctct 260220 tagcgttgtt tttatctgaa aaagactgta tctgtccttc atttaagaag cttagttttg 260280 ctggatacaa aattcttggc tgctaattgt tttctttaaa gaagctgaag atagggcccc 260340 aatcccttct agtttatagg gtttctgctg agaaatctgt taacttgatg ggttttcctt 260400 tataggttac ctggtgcttt tgcctcacag ctcttaagat tatttttctt atcttaactt 260460 tagataacct tatgacaatg tgcctaggca atgatctttt tgtgatgaat ttttcaggtg 260520 ttatttgagc ttcttgtatt tggatgtcta ggtctctaat aaggctaagg aagttttcct 260580 caattatccc cccagatatg ttttccaaac ttttagattt ctcttctttc tcaggaacac 260640 caattattct taggtttggt tgtttaattc tgtcccaaac ttcttggagg ttttgttcat 260700 tttttttttt ttttcttttc tttttttttt ttaattgatc attcttgggt gtttctcgca 260760 gagggggatt tggcaggatc acaggacaat agtggaggga aggtcagcag ataaacaagt 260820 gcacaaaggt ctctggtttt cctaggcaga ggaccctgcg gccttctgca gtttttgtgt 260880 ccctgggtac ttgagattag ggagtggtga tgactcttaa cgagcatgct gccttcaagc 260940 atctgtttaa caaagcacat cttgcaccgc ccttaatcca ttcaaccctg agtggataca 261000 ccacatgttt cagagagcac agggttgggg gtaaggtcac agatcaacag gatcccaagg 261060 cagaagaatt tttcttagta cagaacaaaa tgaaaagtct cccacgtcta cctctttcta 261120 cacagacacg gcaaccatcc gatttctcaa tcttttcccc acctttcccc cctttctatt 261180 ccacaaaact gccattgtca tcatggcccg ttctcaatga gctgttgggc acacctccca 261240 gacggggtgg tggccgggca gagcggctcc tcacttccca gtaggggcgg ccgggcagag 261300 gcgcccctca cctcccggac ggggcggctg gccaggcggg gggctgaacc cccacctccc 261360 tcccggacgg ggcggctggc cgggcagagg ggctcctcac ttcccagtag gggcggctgg 261420 gcagaggcgc ccctcacctc ccggactggg cagctggcca ggcggggggc tgaccccccc 261480 acctccctcc cggacggggc ggctggccgg gcggggggct gaccccccca cctccctccc 261540 ggacggggcg gctggccggg cggggagctg acccccccac ctccctcccg gacggggtgg 261600 ctgccgggcg gagacgctcc tcacttccca gacggggtgg ctgccgggct gaggggctcc 261660 tcacttctca gacagggcgg ttgccaggca gagggtctcc tcacttctca gacggggcgg 261720 cccggcagag acgctcctca catcccagac ggggcggcag ggcagaggcg ctccccacat 261780 ctcagacgat gggcggcagg geagagacgc tcctcacttc ctagatggga tggcggccgg 261840 gaagaggcgc tcctcacttc ctagatggga tggcggctgg gcagagacac tcctcacttt 261900 ccagactggg cagccaggca gaggggctcc tcacatccca gacgatggcg gccaggcaga 261960 gacgctcctc acttcccaga cggggtggcc ccgggcagag gctgcaatct cggcactttg 262020 ggaggccaag gcaggctgct gggaggtgga ggttgtagcg agctgagatc acgccactgc 262080 actccagcct gggcaccatt gagcactgag tgcggttttg ttcatttttt aaattctttt 262140 ttctttgtct ttgttggatt gagttaattt gaaaaccttg tctttgagct ctgaagttct 262200 ttcttatgct tgttttattc tattgctgag actttcaaga acattttgca tttctctaag 26226D
tgtgtccttc atttcctgaa gttgtgattg ttttttattt atactaacta tttcactgaa 262320 gatttctccc ctcatttctt gtatcatttt tttgacttcc ttaaattgga cttcaccttt 262380 ctctggtgcc tccttaatta gcttaacaat cgaccttctg aattcttttt caggtgactc 262440 agggatttct tcttggcttg gatccattgc tggtgagcta gtgtgatttt ttgaggggta 262500 ttaaagaacc ttgttttgtc gtattactgg gattgttttt ctggttcctt ctcatttggt 262560 aggctatgtc tgagggaagt actagggctc aaggctgctg ttcagattct tttgtcccac 262620 aggttgtttt cttgatgtag tactctcccc cttttcttag agatgtggct tcctgggaac 262680 cgacctgtag tgactgttat ttctcttctg gatctagcca ttcagcaggg ctaccaggct 262740 ccaggctagt actgggggct gtctgctcag agtcctgtgc tatgggctgt tttcaggtct 262800 cacagcgttg gattccagca cctgctctga tagaggtggc aggggagtga aatggactct 262860 gcaggggtcc ttagcttttg ttgtttaatg cactattttt gtgctggttg gcctcctgcc 262920 aggaggtggc actttcaaga cagcgtcagc tgtggtagta tagggaggat caggcagtgg 262980 ccagggcctt agaactccca agagtatatg acctttgcct tcagctacca ggatggatag 263040 ggaatgacca tcaggtgggg caaggctagg actgtctgag ctcagactct cctcgggtga 263100 gtcttgctga ggctgtgctg tggggcaggg gggtgaggtt cccaggtcaa tggagttatg 263160 ttcccagagg attatggctg cctctgctgc gtcatgcagg ctgtcaggaa tgtgggggaa 263220 agccggcagt tacaggcctc acccagctcc cttgcaaccc ccaaaaccgg tctcactcct 263280 gtgccctacc aacagcatca agtttgtttg caggcagcgg atgagcaagg ctaagaactt 263340 gccgcaggct accagcctcc cagcaaagaa tgtaagtagg gctttcatgc ctcccttcct 263400 gttgaatctg tacaccaaat tcactccctc ccccaagttc tggccaggtg acttcatgtt 263460 tggttggaat tgttacaaag ttcagctgga ggtttccttc tcgctgtggt cttttcccag 263520 ttcctctggc caccctcccc aaggacccct gtgagacaag gtagaaatgg cttattaggg 263580 gacccagaga gcccacaggg cttttcccct tgctttctct accctttatt tcactcagct 263640 gtctaattaa atcagctcca ggtaagttca tatccttctc ccatgatctg gagctgcaga 263700 ttccccagtg agggtgtgtg ttcgggggtg ggctatcccc ctttcctact ttcacagctt 263760 gggcactcac agtatttggg gtgtctcctg ggtcctgtag gagcaaactg cttccttcac 263820 agggtctgtg gattctcttg gctttcctgg tatattcctg tagtagttct ggagcagaag 263880 tttatggtgt gagtttccac acactgctct gttcatccaa gtgagagctg caatctagtc 263940 CtgCCtCCtt tCCdCCattt tttggCCttg acgtattaca tttattttat gtttgaagtt 264000 ttggcagttt tattttgaaa attttacata acaaatgcca agccaaatcc aaacttcttg 264060 gcctaaatca tttacagata acctttactg agcatttact acatgaaggt gttgttgaca 264120 caggggattt aagatgtgat cactgaccac aacatccagg caacctaaaa gtgaaaagag 264180 gtcattcaca aaaaatagga ccaaacttcc tccattcctc aagggaccta ggactggagt 264240 tggcctaaaa attcctgctg tatcacctca cccaccagtt ttgatcttgc tctcagggat 264300 caaggaagct caaggtttct ggcccttttg gctcatgttc ttggaggtat attctattgt 264360 caagttaaag tcgtacccat cctagatctg tttctctctc tttgtggttc cagtctcata 264420 gaaaacataa ctatgtgaaa acaccaacta ccccataaag ctattctgag actcaaagac 264480 atgaactcta ttttgataat cacaaattga tgtatagatg tcagatatca ttcatgtatt 264540 atgtagcatt gtggtttaag tcttagctgt tggaatcaga ctacttgcat tcaaatttta 264600 gctctgttga taggtgtgta accttgaaca agatacgtaa cctcttcaga cctttgtctc 264660 tttgtttgtc aaataacgac aataataata ctaacttagt aagtatgtat aaggaggcat 264720 gtaaagcttc tattgtattt ggcttatggt gagcccttca tataaattgg ttacaatggg 264780 taatgaaaag tacgttcaat ttttcccaca aggatttaat ctaatctcta aataaaatgc 264840 cagttttagt atactgtcat cattataagg aaactataat tcttaagtaa ttaattactt 264900 tggttccctt tggataatta gtgatacata aaatgcagta ccttgccctg aggaacctac 264960 aagttagcct ccctctaatc ctggcattca aagtcctcca ccttccgaat tacacctgtt 265020 ttttcaggca ttgtctccca ctttcctagc aagcctttgg ctccaggaaa ctgttctgtt 265080 cattgacgac agaatgtatt ttgtcccgta tgcctccctt caatctgaag ttcattttta 265140 gtaatttttt ttattagtag tgactttccc catttactaa tgcctttctc tttagctcat 265200 ttagaattcc ctggaagtca tcatgttttc cttctgaatt gctatcatat tctgtctctg 265260 tgtctaatca gaaagttagg tgagctgctg tctcagctcc tgtgttaatc caggtactga 265320 gctctttgta ccctgccagc ttgagggcaa cttggatgcg ctggtgtgta gtgatttgca 265380 actgtgagag agccgtttcc atcaacaagt gacagctttt ggttctaaac actgcttgac 265440 ctcattctgg tttgaacata tactttggcc tctgccctct acctcacctc cagttctgat 265500 cttgggcatg ccacagaacc ccatctacac taggctttca ttttggactt ctaagtcctt 265560 tacttgacca cttattttct ttaattgcta gacttaatca tccatgcata tgacagccac 265620 tcagctctga ccccaatact tcattgaggc tcatgccagt ctgacctctc acagggaaga 265680 gcacccctgc cggtgagccc ctggcccttg gattatgctg tcccaccagt gcccagctgg 265740 ctgcttggca ctgcatgtga tgtatctttg tttattgatc gcttaattga atgggatata 265800 ggattatatt ccatgttcca gatcaagttg agccaagctg acaacttagt attaaaaaaa 265860 tatatttatc tcctatctgt ccaattaaaa ttttaatttc tagaggtaga gacagtcaca 265920 ttgctattta attcctagca cagcagtatt tttgtcaata tttgttatta gtggtgagtt 265980 taaattataa aggaaaaaag agaagtggtg atttatgtca gcgcttggct aaactattcc 266040 cttgatataa gtctattatt caggtcacat aagcatgact gaagtaatat cacaaccata 266100 atatcataat tatctctctt gctgtctctg tcactttctc acttttgttt gtgcatagtg 266160 catagacctg tagaaaattt attagtaatc tgaagcataa aaccaaatat tacaactatc 266220 ataacaaata aataaaactg tgcactcctt tagggagtag taaagccatt tctaaaatta 266280 agtcaaagta agattccctt attattgaga tattttagtg gtatacacta tcataagtaa 266340 gatgaataat tttgttagca gctgacactg ctatatagat atttatcttg ggaagttctc 266400 agtgtaacat ctttactgtg tttttctctt tagattatac ttttatgttt attattttgt 266460 ttcgtggggg attcaaaaat atgcatttta tgccatactt ggggattccc tataaattat 266520 tagttgtaat atgacagttc catcatgaat tttccaggta ttcttttatg caagcagata 266580 atagctcatt ttggttttta acacattcat gcatattctt tctctctcaa taaatatggt 266640 tttaatttat taaatgaaag atttaaaaat gtgctaagca ttttaataat aatcgatttt 266700 ggattaactt gttatgttta ctctagggct gtagttcacc atttattcag ttaagtcctt 266760 ccccaaattc aatattgaac atgtggaatt gattcgtttc acgtggatgt atcatttact 266820 cccaagatgt tggtttttgg catttagtac tggtaatggg ccaggaaagt gctcatctat 266880 atttgttgtt attcactgat tgcatcttgc ccttgcaccc actgagacga tgggaaagta 266940 gcaacaatac taggtgattt ctttgattta aacccaatta aaagaattag agagttgtct 267000 gatacaaggc caaagaacta agaacagaaa caaaagcaaa acaacaaaca gcagcacaaa 267060 ctccagtgag ataaattttt aaaacattgg gaatatttaa aaaataaaaa cactccaatg 267120 aaccacccag gttttattaa agagtagaga actcaaacag cagaaggcag agctggtgga 267180 ggaaactcag caactgctca gaaatgaaac aacctaggaa ggaggtgcga gtgacctgaa 267240 actcctttta aaaacagaaa ggacaaaaag aggtatgggc tgacaaaagg aaagtggtag 267300 attactgatg tattgcatta tgcttagaat gtctcagaat gcgagaggca gtaaacacac 267360 cagaagagga tacaatatcc ggaccatgtg caactgcaaa taaatgtttg ggttaaaact 267420 tggttgattc taaattacat gaagagctga tgataattga ggcagaactg atagacctaa 267480 caatagaaaa aaatcgattt aaattcagag aagaggtcat ttaagaaagt ataatgaagc 267540 cacttaataa aaggaaatat tcctctaact aaagtttgag atttagggca taatcctcaa 267600 agacaggcta atataatcta ttttttgatt aaaaaaagga acttttggtt taaagtaaga 267660 tgttcagctg ctcttagagt ttatttctcc atatttgggc atataagaat ttaaggaaaa 267720 atatagaaat ataagaaaga tttcatgaga atcacaagca tagtttatag gcaagatagc 267780 cttttctgtt tgaaagcgaa aaatactatt tcaacatgta aaaacctaag tcagttttca 267840 ctggcatgtc ccacaatcat gcctgaaata atggttgaag acagatgtga gacatttcaa 267900 gggcaataga ttaatacatg aaacccactt atgcctagcg ttccattatt ggaacgctaa 267960 gcatgtggga gttatttata tcctattgct caaggtcatc tccaaggtct gagttttcac 268020 tcatgcaaaa attcaaaaaa ttgcaacctc ggcgtaaatg ggttaacaaa aagttaatgc 268080 tggacagtaa aataaactac taaattagac acaccatatt ttttaaatta taagagatta 268140 agaactatga gatatttaaa aagccaccca cagaagtagt aggacaggta gagaaggata 268200 aattctaaca atcaactgta tctccacccc accttgtaaa tgacaaatta gtttacttag 268260 tcaattcagg aaattaaacg tatacatttt gtgttaaaac agaagacttt ttttaaaaaa 268320 agtgtgttag ttaaggttaa ttttgaccta gttaagaaaa agcagtttag agtcttgaaa 268380 tggaaatgag aaatacataa ttacccttaa aaataagttg atagtgaatt ttatctagga 268440 ttcttaagac atttttaata tttaatgaaa atgaactaca caattattaa aaaatagttg 268500 cctgggttta gaaaaatgat cccttaataa gacatataca acagcctgaa tttttatttc 268560 caaatactat agcaacaaaa taccggcaag aaaaatgcac agaaacattg gatagtctct 268620 tcttactaat cttttacaga tatttatata ttctgaattc taacttttaa tttattttat 268680 gtattataaa tgtcatctag tatgcagctt gactttgctt tatggtatca tctgtttcac 268740 agaaaatttt aagttgtgga atttatcgta ttttttcttt atagtttgtg ctttaagacc 268800 tgtctaataa atctttcctt ccctggacgc tataaagtta tttgtctata ttctgttaaa 268860 aggaataaaa ctttgctttt tttcacattt tgttacttat ttcacctgaa agagaatttt 268920 gtttattatg tgaggtagga atataactta atttttttcc attgaataac caaattgtcc 268980 cagagcaatt aagcaataca ttctttccct gttgatctgt gaagctacct ccattaggca 269040 tgaagttgtc ctgttgaaga gaatctgatt ctgagctctc ttcactattt ctttatttgt 269100 ctatgtctac actaaaagca atttaatttc tgtgtcttta taattacttt gactttataa 269160 tccttgaaag ggactttgtt cttcttcaaa attgttttgg ctatttatgg ctacttttgc 269220 tttcaaatga gtttttaaat caaactttaa tgagattgca ttaaacttat aaattaatct 269280 gaggaggatt gacatcttca tgatatttag tcttcctacc cattaacatt atataactct 269340 ccatttattt taggtctttt tgaatgactt ctaataaagt ttttcaattt tctccaaaat 269400 atcttacata ttaaaattta ctcatagctg tctcatctta ctgatatttt aaagacatat 269460 ctttttaaaa attatattat taaattgttt caggtatgtc tttttttttg aagttttaca 269520 aatttgggat tatactgcct cttcattcaa cattttccca tgctgttggg gatccttcaa 269580 gtcaaaatta ttagaggagc tgcattaata ttccagcatg gacatactat aatttattta 269640 attatttccc gaattgtatg tttttgtttc taacttcaca gttttcaaca tgactgggaa 269700 cagcttttct tgctaaatcc ttgcacgcca cagtaattat ctcactgggc caaaagatag 269760 gtactttttc atgactttcg ccatatattg tcaaaatgga accaatggtg atttgttcag 269820 aaatcatggt acatcatata aaaagattac tctgcaagca tcacaaactg tgatatatag 269880 aaatgtgttt attgacatga aatagatcat gaagaaagtg attacaaatg gtatttaagc 269940 attttaagtt atataggctt atactgagca ataacaaaga gggtgaataa atgaatgaat 270000 atgtgattct ggaagggtat gtaacaaaag gttaacaatg gttagccctg ggtaagggga 270060 ttatgtatga cttttatttc cttctttttt tcttcctttc cccttatgtt tttaacaatg 270120 tacatgaatc gtttatgaat aaaataaaaa actttgatat tcttatatag gtttagtagg 270180 ataacatttc tccttcattt ctttcatttt tagaaatctt tcttcacgga aattcttttt 270240 tttaaatgtt tatttctgtc tttgaggagt gagattgact ggctatcttt taatacctgt 270300 gtgtattatc attattgtat cttgctgata aatttcacaa cagttaatta ttttattccc 270360 catttcatac ttagctctat tttaggcaag ttcacaaata tcattgaatg agttcattca 270420 atgtcatcat gatgatattg aaatgtcaat agcagatgac ttgtttctgg ggtgttaata 270480 tgcctgttgg ttacatttgt gttttttggt gatggaaggc agccactgag gcaaacagca 2'70540 agaatacagc taagcctcta actgcatact cttctcactc gttgcttaaa actgaagcgt 270600 ggataattgg tgtgcattcc ctaaaaagtt atgtcatgat gcagtagaga aataagttaa 270660 ctttcctatc cctgtatcaa agtttctttt gaaggtaact gagacagcgc caaataagga 270720 gcagagtttc tgtgaatcat gaaatctttc taaacaattg agaaaaaaaa aactgttctg 270780 aaacaaaaac ttgtgcccta tccttttatt tgaatgtgtt ttcttaaggc cacaactggc 270840 agagatttga tgattttatg tttaaagcaa tttttttttt ttttcatagc aaggagtccc 270900 actgccatca ggttttgttt ttggagagat tctgtaactg acatagggta acttactctg 270960 atggcttgct ccacattcac tacaagtaca tactgttctg aaaaatctca aagttgaaat 271020 atttttattt gggcaacttc tgcaatcaag gtaattcact ctatctttgg agaataaaat 271080 ggaaaagggc aacccaatct ctgtgacctt aaacttccac tgaaacttag agacggttag 271140 cttagctggt gagagcgtgc tgctaataac accaaggtcg tggtttctat cccattacag 271200 ggcagtcaag ctcaagggaa aaaccctgtt ctttggcaca gcttccatgg cctgctagcc 271260 gtttacagcg aggcaaaaga gtagagatgt tttagtgaac taggccatga cttggggagt 271320 ctccaccttg aatctggttg tgggaccaag gaagagaatc tgctgaggct gggtagagag 271380 actctagagc accttaggcc acaggacagc agaggatgag aataacagaa ctggcttcag 271440 aaagtaggtg tcagtccaag gcactgtatt caatacatgt agcaaagaat gagcttctga 271500 caccaggaaa gtcttcagat ggcagtgact gcataaaccg gtgtcaggat gtctgactat 271560 aaaggatggg acaccttcca tcaggtgaga gctctggagg tggagtctgt tcctggaaga 271620 agcatgggta aatgtcatca aaggttccct cagccaccac tgggttcagg gctgaggtgc 271680 acctaggaag gcttgggcag aagcatgacc cctggaactt ggccaactga gccatcagag 271740 agtggtgcag gtgaggagtg gtagatgtgt agtgaaccac tcagctgtct gcccaggcct 271800 tgggtgtgga agtagagtct tctgtgtctt tgggcagact gaattataag cttcaccaga 271860 tcgccaagct aggaatgttg acatacctgg cttggggtca gggagggact gaactgtagg 271920 tggggggccc caacaaatct aatcataaga atctatgagc gtggagacag aaggaccaaa 271980 gaagggatgc tgtaaccaat tatcattatt gaaaaactag gcagagatta cattcaattg 272040 gcagtaccac cctttttatc tctgacatag ttccagaagg atatactatg tatctagtgt 272100 gacactgtct gatagaattg tctgaaatga tggaagtgtt ctgtggctac ccattttgat 272160 agccactagc cacaggtggc tattgagcac ttgatatgtt gttggtgtga ctgaggaact 272220 aaatttttaa ttcaatttaa attaaattta atttaaattt aagtagcaac atgtggctgg 272280 tggttactgg attgaaccga acagatctag atactagagt aagtggtagc agcaaatcct 272340 atattggaac aatgtaaatt aacaattata aatatatgag caggacaaag cccaaatggg 272400 cctgtactct tagttttgct atatattcaa ccataactag cactcatttt ttaaacttat 272460 aaaatgcaaa ctcatttggt tgtgatgatg aatgctcagg taattgttga gtttcaacac 272520 agtgacagcc aggaactcat tttggagact tggattgtgc cacagtttgt ccctctataa 272580 aatgatgatg aaaattttat cagtatctgt ctcaagctgt agttaatggg ccaatttcta 272640 aatattcctg tcaaattgta tagccattgt tttcaaagta gttttgaaag taggccattg 272700 gtaaagcaat ctattaaagt gcaggaagaa agtaatagaa tctcttactt tttaaagtct 272760 cattatgaaa attatatttc agtttgtatg ccttcgatgt acataatata ttaatacaca 272820 aatatattaa tgcagcggtc cccaaacttt ttctcaccag ggactggttt cctggacgac 272880 agtatttcca tggattgcag cggggatggt ttcaggatga aactgaccca cctcagatca 272940 tcaggcatta ttaggttctc ataaggagca cacagccttg agcaggtgca gttcacatat 273000 agggtttgcg cacctatgag aatctaatgc tgccgctgat ctgacaggaa atggggctca 273060 ggcagtaatg ctcgctcact gctcacctcc tgctgtgtgg cccagttcct aacaggccat 273120 gaactggtac cagtctgtgg cctgggggct ggggagtcct gcattaatgt aattaaatat 273180 gtacatttct tgagagtgtt tgttcaaata ttttttccta atagagacgt ataacttaga 273240 aagtatggaa ccctctggtc agtagcatat tttatcaaaa aggctatccg tcgtgctaac 273300 caatgtgatt aaatgttcat tttagtctca gatattttga taacataata atagggaagt 273360 tatggttatt gcatggcttt tctttactgt attttcaata actatattaa tgggttcagt 273420 tgttactagt tttataattc tgttttattc tcaatgagtc ttcctgtttc tccccacgtc 273480 tttcctatgg ttcatttccc tgcatcttta cagctccctg gaacattctc tcaaatcaat 273540 cagaggcttc aaaactgggc agtctcattt tgtaacctgc tctgactcgc ctctgggagg 273600 cctccctccc cactcacagc tgccttcctg catgatgtaa aatgaataca gtgccagaaa 273660 gactgatcct tggaacctct tccttactta gtttctttat agttctgatt ttagaaatga 273720 ccattttact tccagtgccc ttccccacac cagttagctg agactctatg caggaagttc 273780 ccagtgatca gaactgttcc catagctccc aggtgattct gacatgcacc cggcattgag 273840 caccatggac atggctgctg tttgttatcc tcgagctctg caaagcagtg tcggatattg 273900 cttgattgtt gcacattaat ctactgcacg gtccggtagg tagccactag ccacatgtgg 273960 ccatttagat gtaaatgaat ttaaattaaa tagtattata agttcaacac ctcagttgca 274020 ctctctgtat tcaagtgctc agtagctaca agtggttggt ggctaccatt ttgggcagtg 274080 ctactataca acatttctat caccacagag agtgctgatg aataatagta ctaacaactg 274140 gaaacattga aatggagagg tgaaggagag aaggcagaat ataagagaaa cttcaaagca 274200 ttcagcgtgt ctgataggcg ctgtatctta cattatgttt ggagcatagt ttttgaattt~274260 cttaattttc aaattcggtt gaaacagatt ttggtcaatt gaagtgaagg actatgcctg 274320 ctttaatttg cgtagcttac ctttgtttca cgtgtctccc ttattacagt gtttgtagca 274380 gcatctaact caatccctaa cttctttttg gtgcagggac catttaaaaa caatttgatc 274440 atagaggtta tctcctttgc aatccatatt gttttatgga acaggtgaac agctggtgac 274500 aatagcctca aggtttctaa tcccaccaaa ggaaaatatg gtgtcagtaa ttaccttgta 274560 ctatgatatt tgtgaaatcc aagagagggc tagatctcag gctctaataa tacatccgta 274620 taaacatctc agtagggcac cacggaagag tcttaggata tcaggaacaa taagagcggg 274680 agaatatcga ccttaatatt ctttacactt attaaaaagt gaaaagacag ccataaagca 274740 gaatgagttc ctgtcctttg cagggacatg gatgaagcca gaagccatca ttctcagcaa 274800 actaacacag gaacagaaaa ccaaacacct catgttcgca ctcataagtg ggagttgaac 274860 aatgagaaca catggacaca gggagtggag catcacacaa cggggcctgt cgggagttgg 274920 gggactaggg gagggagagc attaggacaa atacctaatg catgtggggc ttaaaaccta 274980 gatgacgggt tgataggtgt agcaaaccac catggcacat gtatacctat gtaacaaacc 275040 tgcacgttct gcacatatat cccagaacta aaagtaaaat aaatgaaaaa gaaataaaaa 275100 ataataaaat aaaataaaat aaatgaaaag tagccatttt accagttgag tatttactga 275160 cttttggtga aaatcacttt ctaagattac tagaaacctt atgaccctcg gcctctactc 275220 ccaccacgtg cggattttca cctccaccta ctttgtcacg tcacatgccc ttctgctctt 275280 taggacactt gtattgggtt cctcccccac acgtaaacag aacacacagg tctgtgaatg 275340 gacctcatta tgctcatttg tctttgccaa gaactattga aaatgatcac aaatgactat 275400 gtaattataa ttaagatttt gtacatgcat acactttatt tttaacaagt ttgtcctgtt 275460 aaatgtcacc ccattttgaa tagtaacatt cactggtgca aggaagaatt attaaaatga 275520 caatttaact gatatccttg acaggtaagc cagttattgt atttcataat tgtgattatt 275580 acaataccta tgaagattaa ttaggacaat ttattctata ggacttggtt atcaaaatga 275640 tcttttttcc cattttagtc catacatctt cagttttaat ttccttcttc caaagaactc 275700 tgagtcttgc ccagtgtgat acttcagatt ttgtaaaaat ataatgaaga agaataacat 275760 gtatcagatc ttttttttaa tgtactgggc taaaaattat aagcaccatc aaattactag 275820 aaaattcttt taacactctg cttcttagtt aaaaaatgat tcctttttgt gataatctta 275880 taattttcaa agtatcattt tagaaatttt aaaaaattat tcttgttttc attcagtcca 275940 tttcatcaga acttagattt tttcttccct ctacagtatg tggtcttgat gtgctttctc 276000 cttgcaaaat aaaatcttta gtttcaggat ctctgtgttc tattccttca atttaaaatc 276060 ataggaaaaa taactatcat ttccttatgt ctgtctccaa ttcccccatc attttctgtg 276120 agccaatttt cttctttttc ttctgtctac tgtaaggaac tttttactct gctctattct 276180 ctgcaattca gggctcacaa tttctttgac ttctcagatt atcctttgta cttgtgtcta 276240 ctttttcatt aaaaaaaaaa aggcttcggt cttattttga ctttatcatc tttatattgc 276300 catatttaaa acccatctgc ttattgattg aaccaaactt ttcatcttac ttcatcatac 276360 tgaatgtata acttctgaat gacttaaaaa tcatcacata atgtgagaga attttctatc 276420 atattcaggt gactgcattg taagcaatga agttgaagaa aaatagctta taatggatat 276480 aataccaaga aataataaga atgagaatct gcgtatatca tatactgggt taacaagctc 276540 acaaggcaaa tcaccatttt attagatatt taacaaatca tactagtact ggaggaagta 276600 tagcagaaaa aaggagtatg gacattggga ttagctgcct tgctaatctt ttgtcagtcc 276660 ctagtgacat gcttttgggt tgagtcattg ttacctccct gagctgtttg tttataaaat 276720 gggggtaaat atctcagatg tttcagtgtt gtgacatata aagcgtcctc catagtacat 276780 aataccgtaa gcactccata aggtaattct tgtcctttta aaaagtatac acttttgagt 276840 tcaggacacg tacactgtaa cctagttgta accattttaa taaaagaagg ttttgatcat 276900 ttttgataaa tgtactacat gaatagttaa cttgcctttc acttcctata ccaacggaaa 276960 ttttggtaac aaatttaaag aaagcattag gttgaatatc ccatctgatt aaatgtcaac 277020 taaatattgt ttctggcttt taaaatattt ctaattcagt tataaaacag gtctattact 27.7080 caggaggctg aggcaggaaa attgcttgaa ccagggagtc ggaggttgca gtgagccgag 277140 atcatgccat tgcactccag cctggcgaca gagtgagatt ccgtctcaaa aaaaaaaaag 277200 gtgaaatgaa aaacaaaaaa gaggggtgaa atttctctgc attctccctt tctgctgttg 277260 ggaaagccct ttcattacca agaatgcatg gaattctgct tattaatttc tccatctatg 277320 tgtctctgtg aaaaatacta attatttaca attttgtaag ctatgaataa ggtctatgtt 2773'80 ttctctgaaa ttatagcaac aggagcattt cccccttatt tgggccgcaa tcttttcatt 277440 ctgattctta ccataacttc tgacttctga ctttatcatg tcttctgatt tctactaaac 277500 atgaacagaa tgaatgactt ctagaaatgg atacaggtgt aatacatata aggccaggga 277560 accacttaat tatctttaca aagtactagg acttcagaaa aatcaaatga tgaaatggag 277620 ctagctttag tatgttctac ttatcattgg actattaatc atgtctgtct gatgagttac 277680 ctaggacttc aactgttttt atagatttaa agaaaatatt ataaatgtat cttacctgga 277740 tttaagactg gacagctgat tttggatttt tttctgctac cctgttgttg atatcactga 277800 catcagcctt tgcattttga ctggaggctc ctttctctct ttctctctct ctctctctct 277860 ctctatagga aaccagtagt tatgtcaggt tgctaagtat tttcccactg aaggacaaat 277920 attctggagc agtatatatt tagcatttga catttgataa acaagcttgt ttgagatatt 277980 ttcaatacaa tttcagcttt ggcaattgta agcaaaggat ccagttggta attggtgaaa 278040 tgaacagatg tggacacgct gcctgcatcg agatgcatta atttttctga aaagtggata 278100 agatcttgtg agaatgaata atggaattca aatcagatgg ggtgagatat ttgatattca 278160 ggaacaaatc acagtataat agatctgcta ctttgtttgg ttttaatggg gagttgctaa 278220 tgctgaaata tagtcagaga aaaggaacgt ggaatttcta tagaggagag cctggctctg 278280 gggtaatact actgtccttg gtggaatgtt gtcttttctg aagacattgg ttcaattcaa 278340 tacatgaaac attcatcgat tattatttag aaaagctaat ctactgacaa gacacagagt 278400 taagtagtga aaatacagag acctagacac agttcttatt aagaaatttt ttaatttagg 278460 tggcatattt cagagagaaa ttctctgctt ggggcaatag gtacctcctt tggaataagc 278520 atggtgagaa agaattgctt ctggcagatt agtgagaaga gtgagatatt ttctttcgat 278580 gacctctcta ttaaaatttg agtggtaaaa ctttgtgatg attcaggctc ttcataattc 278640 ttaattttat catcctctaa taccaggact tgcccagatc aatattttag gaaacagtca 278700 tgcttgctaa acccagggat ttctattaac cactagataa gacataagtt tgtcatgcaa 278760 caagtattta ttgagtccat aatttgccca gcactgggct ttggtactct gggccttgat 278820 actctaggtt gtgtcagaga tgtctaatat atttaaggtg acagagttca caaatgagac 278880 tttatgaaaa tgaatatccc tttttaagtc tcgagaaata tgatgtgcac ccgcttgagg 278940 ctttattaaa tctccagcga gcatgaactt gtttgtgacc caattgtaga attgttgtat 279000 gatgactatc ccactggcag gcacttcttg tccaagagaa tgtaattgga tgttggtgac 279060 tcaagcaatc ctgggaagac tcctccatga ccaaattaaa gacaacaggg gcttggtttg 279120 tactcagctc tacaccaatc agcatgagac aaagaagaga tccatggcaa agtggggaga 279180 ctactgttta ttttacaagc tagagaagga aaactgcagt tcctgagttg caaaactgct 279240 aagaaatggg agaacacaga actttacagg ctgaaccttg gtgtcttagt tattcctctt 279300 ggctacagaa tgccaaccag taggagattc atcactgaga tattacaggg aaatgaagcc 279360 aggcaagaaa aatatgtatt ccctttcttc tcagcccata aattttgtta ttaataaatc 279420 agactcttat agaacagctt gccacggctg tctctagaaa tgtttttatt agcagaattt 279480 ttattaaaat aaaatacaca gtagttttaa gagtgaacat tatagtttta ggtcataagg 279540 ggtgtcatgg agaacacatg tggatgctac ttgccagtta cttgatctag gccttgactc 279600 ttggttttct gttgctcgga taagttagca tgatttgaca tgcagttaaa ggtgtggtaa 279660 cctgtgattg atttcccatt cttgatgctc cctgcctgga cttctcggaa aatttcaaaa 279720 caattcattc ttctatgagg gctgcctcct tggttgcttc ggctaaacag tgttgagcaa 279780 caagttggga aaggaacgtt gcagtaactt ttaaaaatta agttagaaac aaatgtcatc 279840 aaagtaaatg ataatttgcc aatatgattg agcaaaaaga tgaagatgca cgctccctac 279900 gtatttgctt tgcagaagaa ttaatttgaa aaataataac atattttaaa atgaattgta 279960 aatataaaca catgctaatt gcagagggga atccctgtga ttattcagca gtttgtgttt 280020 gtcaagtgct tttcagtcag tccattccag ctcagcaggg cagaggcttg ggctgttgta 280080 aacttgtggg cagataccca gtgatggggc agacatgagc aggtagggct gacagcatgt 280140 gaattgagtt ttcttcagtc atgctgttgc agctgctccc ttccctcatt gctgagtttg 280200 ccacagcagg taggaaccta actctggagc ctgggatgaa ggagaaccca cattgggctt 280260 gaggaacaag atctgccacc ctggtaggcc ctggttaaat ctcatgcaag tgtagcaatg 280320 agagagggta tgattgagtt ctaatttagg aggaaagggg ataatgtgcc ctttgcaccc 280380 ccaggagaaa tcattgctca tctgtgccta agtagactta tcaagggcag ttggttcaca 280440 atggtgtatc accccaaagg actatagtgt tatgagaact tgccagtgta tttgaatttg 280500 ggtgctggca gatgacatca tgaggtatta tggttaccca taaatatgct agtttattga 280560 gaaggtggta gacatgctag tggatgagag ggaaggacag aagcagttag taaacagcat 280620 ctgcaacaat tcagttaact ggtggttgtc acagtagcat ggtggaaaag ttggcaatta 280680 ataacttcta agaaaactga actaatgaac caatcctgcg tgtgctatgt gtataacctc 280740 cttctcacta ttaacagatt tgttccaaac ttatataaga caatgaaaat aaagcttggc 280800 aatataggga agggatggag ggataaagct gtaaatcacg tcacaggcaa attaagatat 280860 accactggat caagggattt aatgcagaaa gactgatcct aacttatttc ttttatttag 280920 caataagatt tgttacttac attgattatt taaaatgagt tgcattatta gaaaggacta 280980 ttttgaagac aactataata aaatgtcagt aactgatagt agccaagata ttttaaatat 281040 atcaaagttg tgtcattaat attaatgtgt cccttaatat gaagtcctgc ccaggcttat 281100 ttatgtattc aacagacaca tacctgttga agtgtaacag atattctgga cacaagcaat 281160 ggtaaacaag acagatgcag tccctgcttt catgcaattt gcaattgaat ggtctttgac 281220 attttattgt gattgtttta gttatttaat tggagaagtt tttaatttaa atttgtgtta 281280 tattcagtgt taaggaacaa aaatgtaatg tgcatttctg agactcagta acacttctgg 281340 tttttccttt ttcatttaaa gaaaaattta gtgcccaaga taagctagaa tttttggaat 281400 caagtaattg atgacctggg agccaatttt attacaatag tgtttttagt ggtcttagaa 281460 cttttcagag gtggtagctc tgaaaataac actgtaataa attcacacat acatctatca 281520 tccaataaat gttaattgag gcccactaca gcattgtgtt agattctgag gttacaaatt 281580 gttgacccca tgtcgaacat gttgacccca tcaatgtaat cagtttgaca ttacacagtc 281640 atttaaacat taaagtgtca gcggatattt tagttgtaat tttgataagt gctctgaagg 281700 agaaaacagt gggtgttgtg aaaagcagta tttggttgat tgattattat agaggatctg 281760 agaaaactta cttgaggaag gaacatttgg ctgaactcaa agagatgagt aggagttaag 281820 taagcaagga agaaaagaag acacatgaag gaggaagaat gttctagaaa cacacacaca 281880 caggtatatg tatatgtaca tgtatatgta tatgcatatg tcttccttag aaacatataa 281940 acagctgcaa catgattgaa cttatttacc caattaccaa agcttatttt gcaccatgaa 282000 ggtggagata aaggcttttt aagcagcaag gatagaatct ttgtagtttt tatttatagg 282060 ctggttcttc tgacaacttt aatttttcat ctttaccaac ttcatggtct tcagtacata 282120 caatgcaatc attattataa aattatattt tgactcaaac tctaaggtag gatgattcag 282180 ctgtgcgcca tcaacatagc agcatgaatg gtagagacta gtcattccaa acagtgaagg 282240 ggcaacgtaa aactaatttt aatattatat gaaagtactt cttgcccttg actgcttttt 282300 tttttttttg aagaaagcaa actttaaaaa tttattttag atttacagaa ttattgcaag 282360 gatagtaaga gagttctcat atatgcctca cccagtttcc tctattatcg acatcttaca 282420 ttatatggta catctatcat aactaatgaa ccaatattgt ttcattatta gtaactaaat 282480 ctatacttta ttcagatttt ctaagttttc ctctaatgtt ctttttctgt cccaggaccc 282540 catcaggata tggtatgtat agttgtcatg tcttcccagg ctcgccatgg ttgtgacagt 282600 ttctgagact ttcattgttt ttgatacccc aggtagttta ggcattttgt agaatgcctc 282660 ccagtctgaa tttgtctgat gttttcctca tggtttgact ggctttaatg tgttttgggg 282720 aggaagacca cagaggttaa gtgtgattgt catcacatcg tatcaagggt acatgccatc 282780 aatatgactt atcactgttg atattaacct tgatcatctg gcttgagata gtatttgtca 282840 ggtttctgta ttatacagtt actcttctcc ctgtccatac agtacttttt ggaagaagcc 282900 attttgtgca gctcattttt tttttaattt taattttaag ttctggggta catgtgcagc 282960 atgtgcatgt ttgttacata gttaaacgtg tgccatggtg gtttgctgca cccgtcagct 283020 catcacctag gcattaaagc caacatgcat tagttgtttt tcctaatgct ctccctcccc 283080 caaccccaat ctgacaggtc ccagtgtgtg ttgttcccct ccctgtgtcc atgtgttctc 283140 attgttcagc tcccacttct aagtgagaac atgtggtgtt tgggtctctg ttcctgcatt 283200 agtttgctga ggataatggc ttccagctcc atccatgtct ctgcaaagga tttgatatcc 283260 ttccttttta tggctgcata gtattccatg gggtttatgt accacatttt ctttatccag 283320 tctatcactg ataggcattt ggtttgattc catgccttta ctattgtgaa tagtgctgca 283380 gtgaacatat gcatacatgt atctttgtaa tagagtgatt tatatttcac tgggtatata 283440 cccagtaatg ggattgccag gttgaatggt atttctagtt ctagatcttt gaggaattgc 283500 tacaccatct tcctcaatgg ttgaactaat taatttacat tccgaccaac agtgtaaaag 283560 tgttcctatt tctttgcaac ctcgccagca tctgttgttt cttggctttt taatgatcac 283620 cattctgact ggtgtgagat ggtatctcat tgtggttttg atttgcattt ctctaatgat 283680 cagtgatgtt g,agctttttt catatgttct ttggcctcat gaatgtcttc ttttgaaaag 283740 tgtctgttca tgtcctcttc caacttttta atagggttgt ttgtcttttc ttgcaaattt 283800 gtctaagttc cttgtagatt ctggatatta aacctttgtc agatgtatag attgcaaaaa 283860 atttccccca gtctgtaggt tgcctgtttt ctctgatgat tgtttctttt gctgtacaga 283920 agatctttag tttaattaga tcccatttgt caattttggc ttttttttgc aattgctttt 283980 ggcagttttg tcatgaaatc tttgcctgtg cctatatatt tattgcatag attttcttct 284040 aggggacttc aaactatgct acaaaacttc aataaccaaa acagcatggt actggtacaa 284100 atacagacac atagaccaat ggaacagaat agagaactca gaaataagac cacacatcta 284160 caacgatttg accttcgaga aacatgacca aaacaagcaa tggggaaagg attgcctatt 284220 taataaatgg tgctgggaga actggctagc cacatacaga aaattgaaac tggacccctt 284280 ccttacacct tatacaaaaa ttaattcaag atggattaaa gacttaagtg gagctcatat 284340 ttaaggagtg agaagatatg ctctacctct ttaagggtgg agtagctcta taaattattt 284400 ggaagtgtct attctcctcc attaatttat ttagtcaaca attagtatca gccatctaga 284460 acccatgaat atttatgctt tgggtacagt ccaatactat tttattttgt agctcatctt 284520 gttccagctt tggccatttg gagatttttc agttggctcc tgtatctctt tggcttctta 284580 catatcattg tagggttttt taaaagcctt ttcttacttt ctgtcactac aagatagttc 284640 agacttatct tctgtatttt ttgccccagt tctatgatca gccacttctc caaggagcaa 284700 taatttcctt caatgaaaac caagatatgg gctgttggtg tacttgttgt tattgtgtgt 284760 tgttacttct agatcctcta agctgatagt gcaaagagat atatgtgtgt gtaccaacct 284820 atatatctac acacatataa aaatatttct atttgtaacc atctgtatct atcttaggct 284880 aaacctgagt acctactgat gtctccaatt ctaacctgca acagcatgga acattctagc 284940 cttctcctct tacttatctg tcacttccta taccaatagt gagaaacctg gctcctacca 285000 tctgctattt atttacttaa ttatttaatt ccactatact tctatggaag tttcagaatt 285060 gttaatctgt actcatgtac gaaacaactt tatcaactag agtatagtgt ttatatacag 285120 ttcctttgcc tttattctaa cagattccac ttactcattt tccgagtcac ttaggttagc 285180 gccttatttt cctaagtcca ttagtgagtt tgcttcatgt atttgtcata catttaaatt 285240 cttttgtaat attgtgcatt ccatcccagt ttcccctgac atcctaaatt aactttttaa 285300 gtttggatac attgtggtct attctttgtt ctgtaaagct ttatggattt tgacaagtat 285360 ttaatgtatt gtatcaccat tatagtaata tagttcctat ggaatagaat agtttctatc 285420 gctgtagcat agaatagttt ctctactcta taaaatatcc tgtgtttctc taattcaacc 285480 cctccttccc accttgaact cctgacaacc cctggtctgt ttaatatctt tcttttgtct 285540 cttctagaat atcatataat tgaaatcata caatatgtag ctttttcaga ctggctactt 285600 tcacttagca atattcatgt aagtttcatc tatatatttt catggtctga tagctcattt 285660 ctttttaatc actgaataat acttttattt atccactcac tggtgaagaa tctcttgatt 285720 gcttctaaat tcatggcaat tatgaatgaa actgctctaa acatttttgt gcaggttttt 285780 gtgtgcatgt gtattttcaa attagttggg taaatatcta ggaattcaat'ttctgcatca 285840 ttgtggtaat aatgtgttta gcttcataag aaattgccaa cctatcttcc aaaatagctg 285900 taccattttg cattcccacc agcaatgaat gagagttctt gatgctcgac atccttgtca 285960 gcatttgatt tttgtcagtg ttttggattt taactattgt aatagatgtg tagtagtgtt 286020 tgattgtttt aatttgcaat ttctttttct tttttgagat ggagtctcgc tctgtcgccc 286080 aggctggagt gcagtggcac gatcttcgct cattgcaacc tctgcctcct gggttcaagc 286140 aattctctgc ctcaagctcc caagtacctg ggattacagg cgcctgccac catacccggc 286200 taattgttgt atttttagta gagatggggt ttcaccatat tggccaggct ggtcttgaac 286260 tcctgacctt gtgatccacc cgccctggcc tcccaaagtg ctgggattac agacataagc 286320 cacggcgtcc ggcctgcaat ttcttaatga caaaaaatat tgaggatatt ttcacatact 286380 ttttttgcca actgtatttt ttttaattaa tttttatttt tattttattt tgtaactttt 286440 attttagatt tggggtacat atgtacattt gttaatacag gcaaatttgt gtcacagggg 286500 tttggtgtac agatcatctc gtcacccagg tactaagcat agttcttgat agttcttttt 286560 cctgatcctc tccccactcc cactctgttc cctcacgtag gccccagtgt ctcttgttcc 286620 cctctttatg cccattggtt ctcattattt atctctcact taaaagtgag aacatgcagt 286680 atttggtttt ccactcctgc attagtttgc taaggataat gtcctccagc tccatccttg 286740 ttcctgtaca ggacatgctc tcgtgttttt tttctttctt tttattttaa tggctgaata 286800 gtattccacg gtgtctatgt actacattgt ttttttttaa accctgcata ccattgatgg 286860 gcatttaggt tgattccatg tttttgctat tgtgaatggt gttgcaatga acctacatgt 286920 gcatgtgtct ttatggtaga acaatttata ttccactggg catataccca ggaatgggat 286980 tgctgggttg aatggtaatt ctccttttag gtctttgagg gatttccaca ctgctttcca 287040 caatgggtga actaatttac actcccacca gcagtgtata agtcttccct tttctccata 287100 acctccccag catctggttt tttttgtttg tttgtttgtt tttttagtat ttaataatag 287160 ccattctgac tggtgtgaga tgatatctca tcatggcttt aatttacatt tctctaatga 287220 ttagtgatat gtagcatttt ttcatttgtt gccaactgta tgtatttttc aatgaggtgt 287280 acatcagatc ttttgcccat tttaaaagtg gggttttggg ctgggcgcag tggctcacgc 287340 ctgtaatccc agcactttgg gaagctgagg caggcagatc acctgaggtc aggagttcga 287400 gaccattctg gccaacatgg tgaaaccctg tctctactaa aaatacaaaa attagtcgga 287460 catggtgtcg ggcacttgta atcccagcta cttgggaggc tgaggcagga gaatcactgg 287520 aacccaggag gtggaggttg cagtcagctg agactgaacc attgcactcc agtctgggca 287580 acaagaatga aactccatct caaaatacat acatacatac atacgtacat acataaaatt 287640 gggtttttgt tttctttttg ttgagtttga ggagtttttt tgtatatttt gattacaagt 287700 cttttatcag ccatgtgttt cacaaataat ttctcccagt ttgtggctta tcttttcact 287760 ctcttaattg tttttttcaa agtagaaatt taaattttaa tgaagcccaa tttattaatt 287820 tttttcttcc atattgtgct attggtgttg tatataaaaa cttactacca aattcaatat 287880 catatagaat tttttctgtt ttcttcaaga agtagtttta taattttgca ttatatgttt 287940 agatcaatga ttcaccttaa gttttgttta aggtgtaagg tttgtgtata agtttttctt 288000 tttccacatc aatgtccagt tgcttcagca acattttctt tttatatata tcttaagggt 288060 aatcagcgca atattttctg aaaagatgac ctttttctca tttaattggc tcttctttgt 288120 caaagatcag ttgaccttat ttgtgtggat ctatttctgg acttcttact ctgtttcact 288180 agtccatctg tttatacttt aaccagtacc atactgcctt tattactgta gcctttatgg 288240 taagtcttga aatgaaatag tgcaagtgct ccaccttctt cagaattttg ttcttcttca 288300 gtattaaagg ctattctagg tcttttgccc ttctttaaac atgttggaat caattgtcaa 288360 tatctacaaa atagattatt gggatttgga tttagattac tctgaatctg ttaattaagt 288420 tgggaagaat tgacatttta tcaatattga ataatatgaa catgtaataa tattgaactt 288480 tcaatctcta ttatctcttc atcattttaa gatcttcttt catttttcat tgttttatag 288540 atttttacat ataggccttg tacatacttt gttaatttat atcttagtat tgaatgtact 288600 attataaatg gtattttcta aactttgaat tcctgttatt catgatgata tgtaggaaag 288660 aaattgactt ttgtatattg acattagatc ctttaaccgt ggcatcatta cttattagtt 288720 ccaggggaga ttttgttgtt gttgttgatt cattggaatt ttctgcatag ataatcatgc 288780 catctgtgaa taaagatgtt ttatttcttc cttcccaatc tatatatctt ttatttcttt 288840 ttttgcctta ttgcacttgc tggtatttct agcataatgt ataataggag gaatgagata 288900 agatatctta gaattatcct catcttcagg ggaaagtggt tagttttttg tcattaagaa 288960 taatgttagc tattgttttt ttaaatttcc tatatgaaat tgaggaaatt tctgtctatt 289020 ctgaatttgc tgagttttta atcataaata gctgttgaat tttgtcaaat agttttcctg 289080 tgtcaattaa tatgatcata tgacttttcc cgttttcact gttaatgtgg cagattatat 289140 tgatttattt tcaaatgttg aatttgccat cagacatgga ataaatccca tttgttcatg 289200 atgtataatt tattttatgc atcgtttgtt ctgtcttgct aacattttgt tgagattttg 289260 tgccagtgct caggagagat attggtctct agttttactt tcttataata tctttatctg 289320 atttgggtat taggataatt ctagactcag aatgagttag gatgtgtttt ctctgcctgt 289380 ttactaacac agattgtaga gaattggcac aatttctttc ttgaagattt gttagaagta 289440 atcttgccac cacctgagcc agatgatttc tttagaaggt aattagttat tgaatcaata 289500 tatttaatat atatagagat atttaggcta tttatttctc catgtgtgag ttttggtagt 289560 ttgtgtattt caaggaattg gtccatttca tccaaattat caaattcgtg agcatagagt 289620 tgttcataat attcctttat tatcctttta atctccaaga gaccagtcgt ggtgacttct 289680 ctttcattta tgatattggt aatttatgtt ttctgtctct cttttttttt tgccagagtc 289740 taactctgcc acccaggctg gagtgcaatg gtgtgatctc tgctcactgc aacctctgcc 289800 tcttgggttc aagtgattct catgtgtcag cctcccgagt agctggtatt acaggcatgc 289860 tccaatacac ttggctaatt tttttttttg tatttttagt agagatgaag ttttaccatg 289920 ctggccaggt tggtcttgaa ctcctggcct caagtgctct gcctgcctcg gcctcccaaa 289980 gtgctaggat tacaggcgtg agccaccgtg cccggcttct ttttcttaat tagcctgaat 290040 agaagtttat caattttatt gctcttttaa aataaccagt ttttgtttca ctgagtttct 290100 ttatcatgtt tctgttttca attttattgg catctgctct aatttcagat gctccttgac 290160 ttatgatggg gttgtgtccc agtacatcca ctgtaatttg aaaatatcat aagtcttttg 290220 acttatgtaa tgcatctaac ctaccaaaca ttatcgctta gcctaacctc ccttaaatgt 290280 gctcagaaca catacattag cctacagttg agcaaaatga tctggcaaca aaacacacta 290340 tagagtattg atggtttacc ccgatgatca catagctgac tgagagctgc ggcttgctgc 290400 tgctgcccag cattaagtga gagtattgtt ccatatattg ctagcacaga agatctaaat 290460 tgaaaattca aaatacagtt tctactgaat gcatgcatat tacttttgca ccattgtgaa 290520 gtcaaaaaaa ataataaatc aaaccatctt aagttgggaa ctgtctatat tattctttct 290580 cttctgcttg ctttaagctt atctagtttt tttcttctct agtttcctta ggtggtggct 290640 taggttggtt attgatatta tatatttttc ttatctaata tatttactta atgctatgaa 290700 tttttctcta agcactgctt ttcctgcatc ccacaaattt tgatgttcta tttttatctt 290760 catttagctc aaaatagttt accatttatt ttgaggcttt ttctttgact catacgttct 290820 ttaaaagtgt gttgttcaat ctctaaatat ttcgatattt tccagctatc tttctgttga 290880 tttctaattt atttccaatt tggtgtgaga gcctactttg tacactttct gttcttttaa 290940 atttgttaag ggtgttttgt gacccagaat gtggtctatc ttggtgcgta ttccatcaga 291000 acttgagaag aatgtgtatt aagttgtggt ttgatggagt attctataaa tattaattag 291060 accatgttga ttcatcatac cgtttaggtc aactatatct gtatta~attt tctgcctgct 291120 tgcactagca attactgaca gcggaatggt gaggtttcta agtataataa tggtttgggc 291180 ttgtctattt ccccttttag tttcaatcat tttgcttcat gtgttttgat tcacttttgt 291240 taggtataca catatataca catatttatg attgttgtat catcttgaac aactgacccc 291300 tttatcatca tctttatcct tggtactttt ccttctttgg tagtctgctt tgcatgaaat 291360 taatatagcc actccagctt tatttttgtg aatgttagca tggtgtatct ttctccattc 291420 ctttactttt aacatatcag agttattaca tttaaagtgg gcattattac taggataaat 291480 accaaataaa gtattttgca tgctgggctt aaaacctaga tgacaggtta ataggtgcag 291540 caaaccacca tggcacatgt atacttatgg aacaaaactg cacattctgc acatgtatcc 291600 cagaatttaa agtaaaataa aaaataaata aataaaaatt aaaaagtaaa aataaattag 291660 ctgggtgtgg aggcagccag ctactcaggg ggctgaggca ggagaatcaa aataaataaa 291720 tcaataagtg gggctttttg taga.caacat atagtttggt ctttcttaaa attcaatctg 291780 acaatttcca tcttttaact ggtatattta aacaatttat atttaaagca agtgttgata 291840 tatttgaata aaaataaacc tgtgtttatt aactatttca tatttgttgc caaacgcttc 291900 tttttggtct ttcgtagttt tatttgagca atgtatgtca taccatttta tcttttctct 291960 tagagtatta actatacttc tttttttttt ttttgagaca gagttttcct cttgttgccc 292020 aggcgggagt gcaatggtgc gatcttggct taccgcaacc tctgcctcct gggttcaagc 292080 gattctcctg cctcagtctc ccgaatagct ggaattacag gcatgtgcca ccatgcccgg 292140 ctaattttgt atttttagta gagacggagt ttctccatgt tggtcaggct ggggtcttaa 292200 actcctgacc tcaggtgatc caccggcctc tgcctcccaa agtgctgggt ttacaggagt 292260 gagccaccgc acccggctta actatacttc tttaaagaat ttttgtagtg gtgccgctaa 292320 agttcacagt atacattttt aagtattcta aatacacctt caaataacac tattcctttt 292380 tacatgaaat atagggatat tataacatag tattctcaat tcctccttac tgtcccttgt 292440 gacatagctg tcatttattt catttcactt acctatatac tataatcacc tatacattgc 292500 tgctattatg attttaaaca ggcagttatt gtttacgtca attaagaatt tagaaagaat 292560 ttagaatcct gtgtaaaata aatttccttt tattttacct taattcatgc attctctgat 292620 tatcttccat gctttatgta gatccaagtt tctgacttat atcaccttcc tcttgcttga 292680 agaacatctt ttaacatatt ctgcagggca agtcagctgg tgatgaattc tctgaatttt 292740 tgtctgattt ttttttaata tttccttcac ttttgaagga taatttccct gcatctagaa 292800 ttctaaattg gtcacttttt tcaacatttt atatatttta cttcactttc tttattaatg 292860 tacggtttct gaagagaaat ctgctgtatt tcatcctgtt ctctatggtt aggtgcttcc 292920 ctgccctggc tctggctttt tcaagatttt ctccctgtct ttggtttttc tacagtttga 292980 atacaatatg cctaggtgtt gtttgttttg tttttttgta aggagtggca tgtatttatc 293040 ttcttgatac tccctgagct tcctggatct gtggtttggt gtctgtcatt aattttgaaa 293100 agttctcagc cattactacc tcaaatattt cttcttcgcc tttctttttt tttctttctg 293160 gtattccaat tatgcatatg cttgttatac cttttgcttt ttcattcttt tgattcttta 293220 catttcagtt ggggacgttt ctgttgactt atctttcagc tcactgatta tttccttggc 293280 cgtgttgaat caattgatta gtcctcaaag acatttttca tttctgttac accattttta 293340 catttctagc atttgctttt gattctttct taaaatttct atctttctgc ttatattacc 293400 catcttttat tgtatgttgt cttctttttc cattagagcc ctcaaactct ttttttttat 293460 tatactttaa gttttagggt acatgtgcac attgtgcagg ttagttacat atgtatacat 293520 gtgccatgct tgtgcgctgc acccactaac tcgtcatcta gcattaggta tatctcccaa 293580 tgCtatCCCt CCCCCCtCCC CCCdCCCCdC aaCaCtCCCC agagtgtgat attCCCCttc 293640 ctgtgtccat gtgatctcat tgttcagttc ccacctatga gtgagaatat gcggtgtttg 293700 gttttttgtt cttgcgatag tttactgaga atgatgattt ccaatttcat ccatgtccct 293760 acaaaggaca tgaactcatc attttttatg gctgcatagt attccgtggt gtatatgtgc 293820 cacattttct taatccagtc tatcattgtt ggacatttgg gttggttcca agtctttgct 293880 attgtgaata atgccgcaat aaacatacgt gtgcatgtgt ctttatagca gcatgattta 293940 tagtcatttg ggcatatacc cagtaatggg atagctgggt caaatggtat ttctagttct 294000 agatccctga ggaatcgcca cactgacttc cacaatggtt gaactagttt acagtcccac 294060 caacagtgta aaagtcttcc tatttctcca catcctctcc agcacctgtt gtttcctgac 294120 tttttaatga ttgccattct aactggtgtg agatggtatc tcattgtggt tttgatttgc 294180 atttctctga tggctagtga tgatgagcat tttttcatgt gtctgttggc tgcataaatg 294240 tcttcttttg agaagtgtct gttcatgtcc tttgcccact ttttgatggg gttgtttgtt 294300 tttttcttgt aaatttgttt gagttcattg tagattctgg atattagccc tttgtcagat 294360 gagtaggttg cgaaaatttt ctcccatttt gtaggttgcc tgttcactct gatggtagtt 294420 tcttttgctg tgcagaagct ctttagttta attagatccc atttgtcaat tttggctttt 294480 gttgccattg cttttggtgt tttggacatg aagtccttgc ccatgcctat gtcctgaatg 294540 8~

gtaatgccta ggttttcttc tagggttttt atggttttag gtctaacgtt taaatcttta 294600 atccatcttg aattgatttt tgtataaggt gtaaggaagg gatccagttt cagctttcta 294660 catatggcta gccagttttc ccagcaccat ttattaaata gggaatcctt tccctgttgc 294720 ttgtttttct caggtttgtc aaagatcaga tagttgtagg tatgcggcgt tatttctgag 294780 ggctctgttc tgttccattg atctatatct ctgttttggt accagtacca tgctgttttg 294840 gttactgtag ccttgtagta tagtttgaag tcaggtagtg tgatgcctcc agctttgttc 294900 ttttggctta ggattgactt ggcgatgtgg gctctttttt ggttccatat gaactttaaa 294960 gtagtttttt ccaattctgt gaagaaagat attggtagct tgatggggat ggcattgaat 295020 ctgtaaatta ccttgggcag tatggccatt ttcacgatat tgattcttcc tacccatgag 295080 catggaatgt tcttccgttt gtttgtatcc tcttttattt cattgagcag tggtttgtag 295140 ttctccttga agaggtcctt cacatccctt gtaagttgta ttcctaggta ttttattctc 295200 tttgaagcaa ttgtgaatgg gagttcactc atgatttggc tctctgtttg tctgttgttg 295260 gtgtataaga atgcttgtga tttttgtaca ttgattttgt atcctgagac tttgctgaag 295320 ttgcttatca gctgaaggag attttgggct gagacaatgg ggttttctag atatacaatc 295380 atgtcgtctg caaacaggga caatttgact tcctct~tttc ctaattgaat accctttatt 295440 tccttctcct gcctaattgc cctggccaga acttccaaca ctatgttgaa taggagcggt 295500 gagagagggc atccctgtct tgtgccagtt ttcaaaggga atgcttccag tttttgccca 295560 ttcagtatga tattggctgt gggtttgtca tagatagctc ttattatttt gacatacgtc 295620 ccatcaatac ctaatttatt gggagttttt agcatgaaga gttgttgaat tttgtcaaag 295680 gctttttctg catctattga gataatcatg tggtttttgt ctttgtagag ccctcaaatt 295740 cctaatcaca attgtttatc tctttcctga cattccacat ccaatccatc agcatgtctt 295800 attggcttta ctttcaaaat aaattaaact cagccacttc tcagcatttt taaaatcacc 295860 ctaatacaaa ccccctgcta cctcatcaac tgcaatggct tcctaactta ttttgtaact 295920 tttgatcttt gagttcttcc aagagccaag agttcttcca aagctataaa ccctatcatt 295980 ggcactcctc tgctctatgg aaagcagtgg cttctcatct ctttcagagt ataatgcaaa 296040 gctctcacct tagctggcat ggccctgtgg gattggcctc ccttgtctta ctttttcctt 296100 gttcaggctg ctgtgacctc tctggtcttt ggctcttact agaaaccttt gaacccgttt 296160 ccttcccagt gtcagtattt gtgtgttgtt cgttcaccct tctcacttca ttctggtttc 296220 tacagcacag agaagtagta gtccctgatc taaacaccct ctccacctgc tttccacttt 296280 gttttttccc ctagcactta ccattatcag atatcatata tttatttgtt tattgtctaa 296340 cttcctcaca aaaatatgac gttgtgagga tagggatttg gctttttgcc ccagagcagt 296400 gcctgactct caataacttt gttgtattaa gtgaatgaat aaaataaaat taaaattgat 296460 tcaaagtgta tgagcatgtg tacattttac ataagtgata catgacattc ttccattcct 296520 tgggggctct tttaaccatt ctcaaccagt tgtagtacct ttaaaaaatc atgatgaaga 296580 tgattttgag agctaatttt ggtaggagac agcagtttta gcctgtccgc tccatgtgca 296640 gaataatagc ctattttatt acatctgata ttcaagcact agaatctatc aataggtaaa 296700 taatttccaa aaataaaagc atcagtggga aaacagggaa attattttta aaaaagaatt 296760 tctagaccag gatgtgtgca atttgtaatc tctaataaga aacgttatag ctgataattc 296820 cagcaattac agaatcatga tcatatccat aatggatcag tagaggctct ggcttatata 296880 atagcttgcc ctctcaggat tctagagccc catatgtaaa cacagaacac atttatattg 296940 attgacagtg catgtaggtt tctacagatt gtgccagtca gtgttttcta aggcagttat 297000 gccatggtaa ttaaaattat gggctctgaa atcagcctgc ctgggttcaa accccagctt 297060 catgtgtcag cttccttggc tgtaataagg aatactaata gcacctgcct tgtggctttg 297120 aaaattacat gatgtaactt tgtcaagagc ttagatgagc acttgggata tggcgagtgc 297180 tcaataaatg ttagtttacc atcatcatca tcattattat tattactgat tgcaagcaag 297240 acaaactggt tctcacttca gcagaaaaag aaattactga agtaattttg gttagatcac 297300 agatttaacg tgaaggatga ataaccagac ttggaaaaca ggtggaaacc aagaggcagt 297360 cagcatggca tagcagccag cacttcacca gtgtggtgcc ttgggggcag cctggcagga 297420 gccactgcct tcactcctgg actgcagatc taccacagga cagcagactg aattgtccta 297480 tgtccagact tcacagtcac agggagcagg ctgcatgcag gtggggccca ggtcacctac 297540 cttcacccta gagtctggag ccacagaaaa cagtaattgt ccttgtagct tttgtgatgg 297600 aaagcaagtc ctgacaccca ccaactcaca tactagggaa ttcaccaaat gtaggacggc 297660 agctaagatg ctgggaaacc aagaattaac aaatgagtat tacaccaatt agttaattca 297720 ttacagattt caatattttc caaaaaacat cctacaaaga acagctcact ttaatatact 297780 ccaacaaatg atgaaatctc tccttggtca tcagcttttc taaatttctg taactttagg 297840 tgattctgaa tttcttagtg attctgaact tctagaagat tctgaaacag aacagtctta 297900 ctttgggatg tattcaaatc ctagagatca gtcacatcaa tctgtgccct ttttcattat 297960 gtaacatgaa acaggaggac ctttccaact tgccttggga acctgctcct acaacagggt 298020 atattatcac atttaataaa gaaagagtaa tatttgcatt gtgacccttc atctgaggtc 298080 atcgggactg tgctgtcctc ccaagctcca ctgtaaaggg atagacaaca accttgtctc 298140 ccagtcattg taatctgtat cagagagcat cattagatga atgaagatgg aaatgttctc 298200 acggtcagca ggtggggaag gcaagacttg ctgattggaa gcaatgaacc atagttcatt 298260 ctaaggcttg gggagagcaa ctttgaggaa atgaggcccc taaacttgcc atgtaggaga 298320 agttgggggg tggtaaattg ggcacccttt atctactaaa cgtaatcttt acttccccac 298380 cctctaattt ttctcagttg ggcttgaaat tgtttttgct tgttacactt tatgggaaaa 298440 gaaagggaaa tcctaataag gtccatgcca gcaaaactct agaagaagaa actaagaatt 298500 tcaaattgac agtttgttaa tgagaaaaga caaggttaaa ggattttgtt aaacagatga 298560 ccaaaataat tattggaaac ttctgcttct ggccaaggtg gaataataag ggctacattt 298620 accttcccac cagaaaatat aataacaaac aaaaactgga caaaacactg gacatgacac 298680 tgttgtcttt aaaccttgga catcaatcag tgcaggatgt tattttgtaa gaaagggaaa 298740 aaaattgggg tgagccttct gattgtttcc agactggaga gaagtttcag gccacagcaa 298800 gtggggagga acctaggtgt aagtatatgt ataaggtagc acatcatagt ggccaaatga 298860 atggcacagg aaagaaaaac tacaagaaca gaggaggtag ggccacaaaa ctagagtggg 298920 gtccaagatt cactatggat aaaatggggt gttaatttgg ttttcaaggc caatacaaga 298980 ggaggcatag aaaaagaaca aggtggtgtt gggaagtagt ataaaaaggg gcacaggctt 299040 atccttgttt tataaaaaac aaaggttttt ggaagggatt tgtagagata agtttgaaaa 299100 gtagtttgag actatgtgtg gaaattcatg aatcttagag tttgatttta ttcaggagat 299160 cagtagcttt taaaatgtaa aataaaacca gtaaactcta tctaaaataa attcttcccc 299220 taatcccaat ataaaaaata catataaaag cgtagctgct ctagacaaaa tggcgggtat 299280 gagatgacca gaaacctcaa cagcactacc tctggtagcc ccatagaact gccaagagtt 299340 ttcagagtat agttaattcc tgctgacggc taaggcagtc attgagtgat tggaaagaag 299400 acggaagata gacaagatca gttaattggg ttggagggaa gactgaagat aggcaaggtg 299460 acaaattagg aactgttgca atgatctagg caagaaggaa cctatgaact aggagggagg 299520 cttaaagaca gcaaggaggg aactataggc agggacatgt gaggaaatgc ttagtagtta 299580 aggtgagttt gtaggtatga aaggagagaa ggaaagaaca ttaagtagca ttaagtgaac 299640 cagataattt atagctcctt gacctgaaac atttagaggc tgtgataaca ctaataataa 299700 taatctataa ttacataaat tacgtagtgt aatgatatat catgatataa tcatcttacc 299760 aatgctttac agtcaatgac attgtggtac agagactttg gaggcagata gacctcgatt 299820 gtaacctctg tgctgcccgt ggtatgacct tgagcatggt agttaatctg aacttctttc 299880 ttttctgtta aattggggac aatgacagta cttaacctta tagtacttga agggagagtg 299940 ttagaattga tactggggag tctctccaca gaaagttgta ttagttattg tctttatttc 300000 attcttaatg aattagcact cccgttttct ttaacatgtt gaatttaaaa cctcttagtg 300060 tatttctttg tcttgccttt taatacccca tgaattattg agaaacaaaa gaaagtgatt 300120 atgcaaatgt ttgaaatatc taataataca tgaacgtagt catgggaaac tggagaaata 300180 actttactga tattatacta gttttttttc tggaagcata gcatattaag aaaactcatt 300240 tcaatgaaag aaaatttaaa aattagagtg cattagaagc ataatccaat gaattctatt 300300 cctaatgaat actcaggcag tatgttaact ttttctgaga tacaatagcc aagccaaaga 300360 atttaaagaa tgaaaaaaac agatgattaa acaaactgtg aagtaattag aggtagtctt 300.420 tgaaaatgcc tcattaggca tttgactcat taggacagat ccttttattt tagggccagg 300480 acataattat ttaagcagtt gatgtgtctt tagctccttt caccttgcca caagttgtcg 300540 cctgtactgc ctttccacct agcttccaag tccaggccga ctttgaagag attccttagg 300600 gCtCdCCtCC CCtggagagt gccctctgta CCatCt CtCt CCCtttCCtC CtCattCCta 300660 ttgcctgagt ttattgtttt aataaattcc cataagttaa cacctcctag ggtggattag 300720 aatcattcaa atccacattg attgttacta agtagaaatt ttatactgag cctttctaaa 300780 tccttacaac aacgtgacga ggatattata gttgctttaa cccaaggaga tgaaattcaa 300840 actggtggca ttgcacactt acagtgggct tgcaggatga aagagagctt ggtatctcga 300900 tcccttatac aaaacaggtg ggtcttgtta gaaaaattct tcaataattg ttaaggttaa 300960 aattttgaaa agtggttcaa agaaatttgc tttgatgcaa atattgtcta gtcagttaca 301020 taacttgagc tataatgaca gtgtacacta gctatccagg gcacgacatc tctattgtgc 301080 tgttgaatat actgctcaac cattctggaa aatggcatgt tcatgtatga atgacaatgt 301140 atcttgtgtg tatggcattc caagccaaca tgttggtccc tgcaagtaaa cttcttgact 301200 gtcaagaggc tgcctgttta atatttgcat gatctaaact aattgtttcc tttgtttttt 301260 tcctctgctt tatgagctgg tagtttgacc ttttggcttt tcccctgaga gttaacaaaa 301320 gttagacagt tggggggtga acttaagtaa aatccatatc actctatgtt gctgctctac 301380 taacaattca agaaaatgtc ttggtagaaa gcaaggaatg agttttaaat tttctctttg 301440 aatttcaatt atccatgcca ctcctgtggt ccaccattct atataattaa gaataagctg 301500 tatgttccat agtgacacag gctgttattt ctgatttgca gttttcactt aacctagggt 301560 aaaattgaaa tagaagtcgc atcctttttt ttttaccttc ttccactcaa ttctagttag 301620 atatttttga acttctataa aatgtctatg caatattatc accaataatg tgcaaaatta 301680 ctactcccat gtagaagagg atccattctt cattgaacca tacccttggg atcctatcac 301740 atgcagttgg ccatataata ttttatatta ttgttttttt gttttgtttg ttaagtttgt 301800 acctacataa tggattaaat tcaaaatctc tgtttaactg aaacaaaatc tgtttaactg 301860 ~3 aagcaaaaaa acaaaatctc tgttttttgt ttgtacaaaa caaaaaacaa taatattttt 301920 gttttgtttg tatacttcat agggactttg gaatttggaa aatatcgttc atttagggta 301980 tatatattag tccattttca tactgctata aagaaatact tgagactggg taatttataa 302040 agaaaaatag gcttaatgaa ctcacagttc cacatggctg gggaggcctc acagtcatgg 302100 cagaaggtga aggggaagca aaggcatgtc ttatatggca gcaggcaaga gagtatatgc 302160 aagggaactg ccctgtatga aaccatcaga tctcatgaga cttatttact atcatgagaa 302220 cagcacagga aaaacctgcc cccatgattc agttacctcc cactgagtcc ctcccacatg 302280 tggggattat gggaactaca attcaagatg agatttggat ggggacacag ccaaaccata 302340 tcagtacatt aaagaaaata tttgttagcg ctaagtgaat ctactttcac ctacttgatt 302400 atttgatgat tttataaata gtcatctctt tcctcatctt tccagtcgta ttttgttgtt 302460 ccaaagccaa aacctgtgtg tcttgtattc aacaacctga atcatacagt tgtgagacgt 302520 aaatgggtgt ggtggttcca cttacttctt tacatagtac tacaactaag ccatctaagt 302580 agtctgctcc taaattcctc caagaaggga aatatcatga tttgatttaa atgtgacatc 302640 ctttcttaga gaagttttct ttgtctcctt cttccccagt cacttcctcc catccactgt 302700 gctttcggca gttcattcgt tcattaattc attcaacgaa tattgaataa cttttgatca 302760 atgtcttcct ccctccaagc tgaaaacttg atggagggta acatgatctg tctttgttta 302820 ccactgcatc ttccgtgaat atacattgta agtattgaac ggatttttac taaatgaaca 302880 aatactttga taaaatattt ttgattactg ccaatatcag tttctgaatt gattctaaaa 302940 ttcttctgct ggaaagtaga ttagcgttag tatgactgct gaagcctttt aaagtgggtg 303000 gtaatactac gtttcgtttt gtttcattaa cctaagtgct gttctttgga atctttttaa 303060 agtaagataa attacattca tgaaagaagc atattatttt taaagtactt tattttggaa 303120 aggtaaaatg cttgtgtagt tataatttgg ttactcttga tttcacctta ggaaaaacaa 303180 tatcaccttc taaccatttc ttttttagtc aaatctcttg cttctatttc tctctgtaga 303240 tccgctatta aagactgtaa tcactgctgc atctttcctg taaggcttga tcgcattgtt 303300 aatttctttc taaacttgta gagtaggtgt ataaatcgta tttgggtaat acactgacta 303360 atactgaaga accaggcatt ttcttacccc agtcctcaca cgaagtaggg aaatacaagt 303420 cagaactta,t cttctcaaaa ctctgacctc agaatttcct gagaaatctg aacctaaaaa 303480 gattctttgc ttttgacatt ttttctctgg tgtccaccgc aaggctcttg ctcttacatt 303540 tttttttttt ttctaatgtt tcaaatagaa gatggtagag tcatatagta caagctcagc 303600 atcggagggg ctactggagg tcaactagtg caaatgcttt ctgaataatg gaatcctttg 303660 ggaatacccc cttgaaagtc ttcatctagc ctcttcttgg aaaccctcag tgatacccct 303720 catcgtctcc tatgatggca gcttctatct ttgtcgtcag ctctgactat tacaaactgc 303780 ttccttacat tgagctgtaa ctaactaaaa agtttccaca ccctgcttct attttagcct 303840 ttgggctcat aaagaacaag ttgaacccgt cttatgcaga acaacccatc tacgggagat 303900 gctcaggttt cctccatgtt ttcctttctc taggctaacc cttcgttgtt cctcacttac 303960 tcactatata acctgatttc atgtctcctt acccttcttg ttactctaac ttgagtaggc 304020 tagtttcagg atccagcaat aagtgtgata catcaggtat gctctgatga gttgagagta 304080 gagtggacat agcatctccc ttaattcaga tattgtgatc taattggcaa atctggcaat 304140 aaaagttgaa atgcctgatc caggacaatg gctggtcagg tgccattgtt ctcatctttt 304200 acttttaggt gtccctcaat ttgttaagtt agtacctacg taatgtcctg aaacttgtta 304260 agtttgtacc tacataatgg attaaattca aaatctctgt ttaactgaaa acaaaagcaa 304320 acttcttttc agagccagaa tctggaatca tacgtaacag agaatgatat tgtacaagtt 304380 gcttcatctc tttaagtaac ggtttctcaa acttacaaga ttattgtgag aactaaatta 304440 gttctaaagt gcttccatgt aaagtgtatg taaatgctta aaatatatag taagtgctga 304500 atgcatatta gaaataataa taatctttat tataattttt actatttcat gagaagtact 304560 tattttaata ctgagcaaat aggaagagtt catggcttct ctatgttttt gaagtgtcat 304620 ttaatatatt atatatataa aacatatata tatttcagtc acacatttgt ccaaatacct 304680 tgacaaatta aaacaaaata agacaaaatt ctcacgctag ttatttgtta taaagtaata 304740 gaacaagtga tatgttataa agagcattta tttctcatgt ctttgatatt aaaaatagtt 304800 gtattaactt tttatcaaaa cgattgcttc cttcatataa atctaagaat tatgctgtct 304860 gataaatatt ggagagatta acttctttga aaatatagaa gctttttgtc tttttaaaat 304920 agttgttttt ttgcagatgt taatacattt cagcagtaca gtatggcctt tttcaggtta 304980 aggtgctgag cccaaacctc aaagaatcac tgcaaaaaga ttggatcccc cctcttcacc 305040 ccatttcgta atttagttag tgagaaccac aactggctaa acctttgtgg ggggccgggc 305100 actgtggctc atgcctataa tcccagcact ttgggaggcc gaggcaggca gatcacaagg 305160 tcaggaaatc gagaccatcc tggctaacac ggcgaaaccc cgtctctact aaaaatacaa 305220 aaaattagcc gggcatggtg gcaggtgcct gtagtcccag ctactcagga ggctgaggca 305280 ggagaatggc gtgaacctgg gaggcggggc ttgcagtggg ccgagatccc gccactgcac 305340 tccagcctgg gtgacacagc gagactccat cttaaaaaaa aaacaaacaa aaacaaaaac 305400 aaaaaaaaaa ccacctttgg ggggaaatta tcaaataaaa caaactcttt ttagaatttt 305460 acaactttta tgttaggaaa aaacaaatac atttgtgaaa agcttaaaat ccagtaaatg 305520 ~4 acttgaggga cttggggcaa tcctagggtg atgaggagca ggttagtaac agtgaaggac 305580 ttagcacccc agggggccag aggctgtaat ataccttatg agcaagtcat-tcttatttag 305640 tcttgcccat taagaagtct acttggacta aatgctttta aaaatgccct tttaatttac 305700 tattaaaaga atattcctag cagaagtagt cttggatgct aaattctatt ttaagaataa 305760 ctaaaattag aattctgttc tttttataac acctgttaca cacacacccc tacctagtgt 305820 gtcggaatca gtttgtatgg gctcaccaaa gcctactgtt caattttcag gagttttgta 305880 agccatttga tgtcagacaa gtggcctgaa gtttgttatg gtggtggtat ttacaccatg 305940 aaaattggca tgttatggtg gtagtattta caccatgaaa actgctacaa atagaaatct 306000 ttttcttctt ctcttggaga gccacttgtt gaacacttac cagctcacct gtgcttgaaa 306060 gtatttcttc aaataaaatg aaagctggtt agctttgaaa attttttgta taaaagttta 306120 cacgggaaaa aaataaacta attttttttt tccacctgtg ttttcaggga tacgaaaaga 306180 ccgaagagga gggagaatgt tgaaacacaa gcgccagaga gatgatgggg agggcagggg 306240 tgaagtgggg tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat 306300 caaacgctct aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc 306360 cttgttggat gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag 306420 tgaagcttcg atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat 306480 caactgggcg aagagggtgc.caggtaagaa tgcgaagcgc agcttttaag agtcaatagc 306540 ttttcaagaa cttgttgtga tgtcatggga gaaatagtgg gggaaaaaga agcaataaca 30600 tgttatgtaa ttggtttcaa ggttacagga gatgtgttca ttttcagtat caatacactg 306660 taattttcca ggagattagg aaataatatt tttaaatcag aatctagaag actgaaattc 306720 ttaaattgac ataatttatt tttaacccat ctcatttacc aaaaagattt agggtggaca 306780 ctacatggta aaactattta atagtgtatg ttcacagtag cagaaacttt taacactaaa 306840 tgaactacaa aagtttgtaa tattaatgac ctttgttgaa aacatctcaa ttattaatca 306900 aacgatttta tcttaaaaag atttttaaga ttcggtgtgg tggctcgtgc ctgtaatcct 306960 agcacttttt ggggctgagg tgggaggatt gcttgagccc aggagcttga ggccatccgg 307020 ggcaacgtgg cgaaaccctg tctctacaac aaatttttaa aaattagctg gatgcagtgg 307080 cacacacctg tggtcccagt tatgggggag gccgaggtga gaggatggct tgagtccagg 307140 aggtcaaagc tacagtgaac catgtttgtg tggagtgcca ctgcactcca gcccaggtga 307200 cagagcaaga ccgtgtcata aaaaataaac cacacacaaa aagagaaaga tctttatgga 307260 ttaaaaagat aataataagt gtatttactg aatgccaatt atttatccaa cctggtgtat 307320 gcttagtatt ttaggagaaa gagaaaggca atggaaaaat aaattaaggt atcatccctg 307380 aaagaaactt ttagaaagac acagtggctg aagtgatacc ttgttccttc agttgattct 307440 ctcagaactg gtgttctggt aaaattggac tgttactcct gttattcagg gagaagaact 307500 caagtttgta tggcaacaag actagaaaat gactttctcc ctgccccagt gtattcgttc 307560 aggagctaat gtagataaac cgaggcaaga agaagctaaa ttttttttct gggcttatag 307620 gttaaatgag tgatagattt agttggaggt tttctcattt ggtttctttt aatagatgaa 307680 attaattgtt tcctatgaag catgaaatgt tttatatgaa actaaaaaaa tgtggagttt 307740 gtacttgcat ttcaagggtc actgctctgt tataggccaa gtgaacttat gtctggcctt 307800 agagaatctt acatgtattt gcatctatca gtatataaac atgtgggccg tagaataagg 307860 agccagcagt accagaaccc agccttgtta gaggccacca ttttggtggt tgagtggtta 307920 ttagtttaca tggaagcatg gagaataata ggcaaatgta ggttttcagt gtcagtcgaa 307980 ctggcaaaca aaaatttctg gtcatcttca gaatgaaaag ttttcttgag tacctacata 308040 ttttagcatt ttcatatgaa gcagatacat tataagttaa ttgttggaat ctaattgtaa 308100 tatggctgta agttttttct ttatatttgt tattgccttg ttcttatatt atagggaaag 308160 agaaacaaac aaacaagcaa agaaactaat ggtcatatat ttgagagcca actcttggtt 308220 gctgttcagt tttattttcc tgtaaacata tattttccct tatgaaatct tgggaatatt 308280 agctctggag cactgctgaa ctcaagtaca gacattttca tgtgtatcag tagattccca 308340 tcatgacatt tttataataa tgttgaagag cattttaaac aactggaatt aagctcaaat 308400 acattaccag tggttgaaga attcacatca ataatcttct gaatttagga ataaaatgga 308460 gaagtcaagg aaagccaaat attatacaca ggctagcaat agttaaaata caattattaa 308520 agccagagct agacaaaatt atggcaatga gatgtgtaac aaaaccactc tgtaacttca 308580 tcatgttcgt ttaaaagacc tgaatgattc caaaatcctt agaccaataa actatgtctt 308640 cttacttgat aattaaaaaa aagaattata aaggcaaaaa tgaaattata tgtatgtgtg 308700 tttgtgtgtg ttccatggga atacagctgt tgtaaatcaa aggcctactt gcctgaccaa 308760 gcagaataaa aatagtcatt gattttaaag agactaaaag tgagggaaga aaaaagtctt 308820 ctgcaaaagc tctacagatg gttgtcaaac ttttcagaaa aatagacaaa gcaacatttt 308880 gaaaaggatt tatatctttt atatattcaa ataccatctg ttatttaaaa aacatagccc 308940 acactgaata tctgatatag acaatataac gtttatgaga taatagtttg agaatgacaa 309000 taataatagt aagttttaaa agaggaaata agccgggcac agtggctcat gcctgtaatc 309060 ccagcacttt gggaggccga ggcgggtgga tcacctgagg tcaggagttc aagaccagcc 309120 tggccaacat gacaaaaccc tgtctctaca aaacagtaca aaaattagcc aggcttggtg 309180 gcacacatcc atagtcccag gtacttggga tgttgaggtg ggagaattgc ttgaacctgg 309240 gaggcagaga ctgcagtgag ccgagacccc acaactgcac tccggcctgg gcaacaatga 309300 gactctgtct ctaaataaat aaataaagaa ggaaatagta aacgtcatta gtaagaagaa 309360 tagcaaaatt tcagttctag aaatacccag aattagctat atgatacaaa aaccctcatg 309420 aaacatttgg ataatcctca gaatacctat caatataaca aaaactatga attgacttca 309480 ttttgaatga tgaattttta acaaaaatag tcaatacttt gggcttagta gctctagaag 309540 tgcttctttc ttctttcttt taattaacag aatattgtca gaattttcaa atgttgatga 309600 ttataagttg aattttcctt ttcattaacc cctggggctt attttttgac ttgatatgtt 309660 tctctactca gaattaagat gtgaagagct ttgggataga atgcatcata caagtgtgag 309720 agtcaagttc caatccataa agtacttctg gtagacctgg ataaagatga ccttaagaaa 309780 tgattttttt tctctttgca gtttaaaaac aacagtagca acaagaatga taaaacctat 309840 gaagccagct ctgacaaaag atgttttttt ataacacata ctatgtatct actttttgat 309900 atttatctat cgagaagact tttctcctta ttgtttgctc tgaaatttgt ttatatttaa 309960 taggaatttt atagccttat ccctgggtag aaattcagtt tttttaaaag tagataatta 310020 aatcttaatt tactattatt ttacacatgg tgaaactagt caataaatgt aatatcattg 310080 agctgaaggt taaaaaggaa aaaatatgac tgcaagagtg gttgtatttt tgatttcagc 310140 atcacactca attgcatcat tgagtggttc attcttagtt tcagtgttaa tgaaatacat 310200 ctgagttttt ttccccagaa gcttgaattg tgtcaccaaa gtgtcatttt gatttattga 310260 aagttaagcc ctttccaaaa ttcaccataa ttttacatgt ctcgaaagca attttatact 310320 tcaagtctgt gctatagttc tatatatttt atgaagattt ggatagatat ctagccctga 310380 gttttttatt gcctgttaaa tacttataac ccaaagtgta gcagcctcat gaactgctgc 310440 tgggcacaga tctgggagag tgcactcaga gtgttggccc aggaagagga aaagaggaaa 310500 aggggcagtt cagtggcagc ttggcttcct gtgaaacatg tcaccttaac cacttcctca 310560 ttcttttagt ggcaaactag acgaccttct ccttcccctt tcctgccgag tccccctcta 310620 atcaacactc tagaaggccc ctcttcctcc tcagtcctga ctccctgtca tttacttcag 310680 gttgcctcct tgctcagttt tggccccagg ggtaggcaat ctcctctttc tggacatgtc 310740 tcccaccagc cccaagttca gatttcttgg aagtttctgt gttgtctcga ggatgcatgg 310800 ttgtgtgagt tattccaggg ctcaaggcct cttctgtgtc tgtattcccc aggaatccag 310860 gaattcactt ctctgcctct catcctcatc ctcatcatta aaatgagcag atttgagctc 310920 aggaagtgct tgaataaatg aataaatgaa caaatgaaag gtgatttttg aaagtcttaa 310980 ttttaagaag tctctgagcc ctgttacagc tcaattttcc cataaactag aactgctctc 311040 taaggctgtg acatttctcc tttttcctct cctcaaaatc tacctgctgt tctgatatct 311100 ccctgacagc caccatagtg agattctatt tccatttcca atctctcttt tgtaagggac 311160 agagaaacag ctagagaaat atggagccat gcgctctgag gactttagca ggcttcaact 311220 ctatctgcaa gtgagtttca cttagcgaat gaaattggaa acttaaagtg ggtagggatg 311280 agggttcccg gagaaggtgt aatacctcag tctgggaatt gggagcatct acaaggaaca 311340 cactcaattc tgggaggttc ctgtagattt cagagattta gcagggcttc ccagcactct 311400 gcttcccaac ctgtcattgg accagtaaac cctgctctca aactgttgtg gtgtccagca 311460 tgctttggca aggtaatgaa agataacatg acatggacat atgggtgaca tcctggagag 311520 atgacagaag tctctgttta aggacaagat ttgccattta gattttgcac ccactgtata 311580 ataagagcct taggattggg ctggaatcgc cctagcaggc atgatggcag cccctctgga 311640 ctggcaatat gcagcttttt tgcaagtgtt ccatgtccaa gtcaccccac ccagctttct 311700 actgctcccg tggaagctct ggtgaaacgc aaggaaagca gctgctgttg actggatttt 311760 tctttcacat gaaactttga agcctcatag atgcttattg gcctggatgc tattaaacct 311820 ttaaaaatcc ctttctcatc ttgagagtat ttgagaaaca tgtctggggc attttgccca 311880 ccctcctcca ggttctgtgt cagtgagtga tatggtttgc ctgtgtcccc acccaaattt 311940 catcttgaat tcccatctgt tgtaggagag acccatggca ggtagttgaa tcatgggagc 312000 aggtctttcc catgttcctg tgatagtgag taagtctcaa gagatctgat ggtgttaaaa 312060 aggggagttt ttctgcacaa gctcttcttc tcttgtctgc caccatgtga gatgtgcctt 312120 ccaccttctg ccatgattgt gaggcctccc cagccacaat ggaactgtaa gtccattaag 312180 cctctttctt ttgtaaattg cctactctgg gatgtgtctt tatgagcagt gtgaaaacag 312240 actaatacag tgagtctggg tcagtgtgtg tttatgttga acgtagtgga cttgtggtgt 312300 ccccagggca ccctgtgggg aatgttggcc tgtggctttg ctacttccag ggggatttgg 312360 catggagaat gtgtgtttta agtaatagat agattatgat tgaagtgtgt tatgggctga 312420 gttgtctctc ctcaaaaaga catgttaaag tcctaacccc cagtatctca gaatctgacc 312480 ttctttggaa atagtgcctt tatataggta atcaacttca agtgaagtca ttagcacaag 312540 ccctaatcca ataaggactg gcattctaat gaaaggggaa atttagacat agaaacagac 312600 atgcacagaa gaagatgata tgaagagaca caggaagagg acggtcatgt gactggagtg 312660 ctgggtctgc aagctgagaa atgccaaggt ttactggcca acatcggaaa ctagaagggg 312720 caagagtgga ctctccccct cagagagagt atggccttga tttcagactt ctagcctcca 312780 gaactgtgag gcgtacattt ctgttgtttt tgaagccacc tagtttttga tactttgtat 312840 gggagcccta ggaattaata caaagtgtat taaaaataga attttcttct attgtatttt 312900 tgaggcaaaa gaaataagga gctattgatt taaggaggaa ggtttggctc atctagtata 312960 gacttggcta atcatttact cttgtatatt tctcttctgc ctgcccaagt agttcactga 313020 acaggcttag aagttgattt ataattgtaa gagtttacac atcagaagtt aatttataat 313080 tgttaacagt ttcatgcatc tacctcataa ataccttgta aaagatttta cccttcagaa 313140 taatgttttc ctctggtact ttagtagttt atcctttttt tgcatctttg atgcatttga 313200 attcatcctg gtatgctgtg tgagctgggg ctccaatgtc gttttcctac agatgactcc 313260 acagttgttt cggtttcatt cgaacagcct cctacagaag tatttttcag acttctttta 313320 tacttgactt ccaagtttac tactggttaa gagcttttaa agtaggggga aagatatgac 313380 ccattggcct actcgtttga ttattttttc caattctgaa atcaaaaatt aaaaaattta 313440 ttcaaagtta tagtttcact taatttatat taactgcata gatagtgtga ttcatctgtc 313500 ctgcttggac atatttagta acttttaatt aatcttagag ataaaagata gaaatctatg 313560 agacttgaga cattcaaata agaccagtac tgataagagg taatcaggtg aagtgccatt 313620 tggttgtaat aggacatcaa aattgttccc caagagtgaa ggatgttcca ttctcacttt 313680 tcccatctgt cctcccaaat tctttcactc ttcctgatgc tttgaaccaa cttgaaaagt 313740 tagtccactc agaacctcag ctgttaaact tttagcctct gcaaacaaac tagccgtttt 313800 cctctttgtc cctttaccat caagctcagt tgcttctcct actcctctcc cttctgccag 313860 gggctccacc cttgttcctc attcaacagt ggaatcatct gtgtgcctgt ctcctcttta 313920 ctgtgtacca ttgggtcctc ttcttcacca tctctaaatt tatgcttctt ccctgttcca 313980 ctgtcaccag cttggtctac atcctacttt gatcatttgg atttaggtcc caccctaatc 314040 gagtatgact tcatcttaac ttgattgcat ctgcaaagac ctgatttcca aatacgacca 314100 caagtaaagg ttcaggtgga catgaatatt aggagagaca gcacttcaat taacagtttg 314160 gcattaggac tttattatct agcttagtaa ttgttttaac agataaataa tagtatcccc 314220 taaagtttaa aatgcagaac cagattatgc ctcagggtgc ctaacctgag aggaagagtc 314280 tttcttcaac agactctggg aaagaccttt gtaaaaaggc tggtgaccat tctatttgat 314340 ttctactttc caatattaac tgcttaagtc agacaatctc cttggcccac gctttcccct 314400 gccattctcc ccttcacata atgtcttcat tttatttttc ccttgtttta ttccctgaac 314460 tcccttctct ctattcagat tccctgccca ttttttctcc taatgagacg cctgacttcc 314520 tacctccttt.ttgaaacttc aaactgctta gcaaatacaa ttttgaattg agtttgctgt 314580 cacagaacaa aaactagtcc ttggtccaga gtatcttgca ggactgaatc aactgtggtc 314640 taagacacaa taataactct taattatttt cttgtttcct tcatggtata tttgttgctg 314700 gatgatgagt ttatattatg gttgaatttc ctttttgaaa tgaaaagatg aatagtcttt 314760 attggctggg tatctatttt tatccagaag actttgcaaa tgagaaatat ttctacaatc 314820 tgagtttaat ttgcttaagc aatgaacttg ttctttaaaa gtcgtatatg tgcaaaataa 314880 atttaaatta aataaatcat attttaatgt agtcagggta gctttcattt aaataaaacc 314940 tatgacaaat atccacgtga aggtatcact cctttaacaa aacaagtgtt ctcctttttt 315000 attgaaatac tagttttcac aaattgagat tccttaatgt gatgaccgct tggactgact~ 315060 atagtatagc tgcgttctta gaatggccct gaaaccacaa ggcctctcca agatgcatgt 315120 ggctgacttt ctgaggtgac tgcattgaat ctttctggct tagcttattc tgcatccaga 315180 tccctggaag ctatttatcc ccaaacagtg gtatatttag acccattgtg tgctaacctc 315240 tctgtgccct aagtaccaac atagccaagc caaagttcga actttgctgc cttacaatgg 315300 aggttacatc gtagtgatga aagaggttaa ttttctttag gagttgaaga ccagagaact 315360 gcaaaaaggg gagaaaatca gtgtggcctc taatgagagc taagtgagtg aggcaataga 315420 aaacagtgag gaccggccag tgcggtggct catgcctgta atcccaatac tttggaaggc 315480 tgaggtgggc ggatcacgag gtcaagagat caagatcatc ctggccaaca tggtgaaacc 315540 ctgtctctac taaaaataca aaaattagct gggtgtggtg gtgggtacct gtagtcccag 315600 ctactcggga ggctgagcgg ggagaatcgc ttgaacccgg gaggcagagg ttgcagtgag 315660 tcgagattgc gccactgcac tccagccgga gtgagactcc gtctaaaaaa aaaaaagaat 315720 acagtgagga ccttggaggg aatcttacag tggagcagaa ttaaaagaga agaattcacc 315780 tatttcctat ataactccct ctgtaaccga taactttcta gagaaagctc actgaatata 315840 tttgaaattt gtttcatacc acttttatgt tattgccttc tttcacagga accagagagc 315900 tctaacctgt agatttgctt tgttgtcttt actttagact acagcctaaa ctgactgttg 315960 atttttggca caattattac atctctcctt tcaccccgcc tctagccctt tcttaattac 316020 ccccattagg catttttctt gctagcgtga actaaatccc ctgtctcaag cacgcgcctg 316080 tacattgaaa tagaatgtta attcattgac gaaattaact gttcttgatt gggaacccct 316140 agttccaggg aatcttaagg tttcaattct gttgccttgg ttactaaact gttccatgca 316200 gtttgctcaa ctttgaatgg atacttctct taggaacatt ctcccttgta agtaaattgc 316260 ttgacgtgtt aaaatctaag ttctgtttta caattcttga gacaaattca ccatgattta 316320 gttttaaaaa caagtggcct tgtcctttta gtctaggatt tatcacttag ctgtatctag 316380 caaagcccct cacacaatag taggaaaaag cagaagtgtg attgctaaat gaaggggaaa 316440 tgtaataaaa tgatggaagt gccctttttt agggggaatt aaagtttctc tttgtagtgt 316500 tacatgtatt cattatgtga ctttcataaa tacaaacccc aataaaacgg tgggcagttc 316560 cgtctctgga gatgttaagt gtctcttcat agaataacat ggcacatcat aacatttgat 316620 tccttttacc tggtcaagat gtttgcttat ggcatttgaa atgcatattt tcaaactagg 316680 tagatgaatg gtttaaatat aaaaattcat tcaaggccct gagctataga agaatatctt 316740 ccaaaacatg cattcaagtt ctatctgtca gtgtttttct ttttgcatca ataaggcagc 316800 aatggaatac aatcagattt attttttctt tattgctgtc tacaattcag agatataacc 316860 tgaaaaatat gttgtctctt cccaagtaga tcttgtaatt ctctgaactg tgatccagac 316920 caatgccgcc tttacattgg gtctgtggaa gtgggagggt ccactgaacc tggttatgag 316980 aggtttcaaa ttgaaaagat ttacaagtat gtaaataaaa tcaaatagtc aattagtatc 317040 taacatatac atgtcaaaac acatagcccc aatctcataa aatcaaagag atctacctaa 317100 atagatttgt ttgcctaaca aatagcaaga ccactttgac agaatcttta gcatgacagt 317160 gattgtcaaa atgggtaaat ggttattatt gcccagttaa gtaatttctg atttgctttg 317220 ccttgattac tcttaataaa ctaaacttaa cataccatag aagcaatatt ttttaaaaag 317280 agggtatatt ctagagctag atagggtgtt gtgcagcaat gtgaatgtac ttactaccat 317340 taaactgcat acataaaaag ggttgaaatg gtaaatttta tgttatatac attttaccaa 317400 aataaagaaa agttataata ctttccgtag catatataaa aaagaaacag acatttcata 317460 gcaaaaatag gataaaaatg atattaaatt cataggggat ttctcttatc taaaacttta 317520 gatttgattc ttagataaga atataaacac attgcaagaa gataaatcaa taagccaatg 317580 aagtaaggtg gaataaataa atagaaatag gtgatacttg cggctctgac agcatctgat 317640 aataatagta caaacagttt catttcattt aacagttatt tgatgaactc ctgtgtgcta 317700 ggaactgtta tttaaaaaaa gtaactttta ttttaggttc aggggtacat gtgcaagttt 317760 gttatatagg taaactcatg actcggggct tggtacacag attattttgt cacccaggta 317820 ctaagcatag tacccaacag tatttttttt tccgaacctc tcctcctctc accctccctc 317880 acgtaggccc agtgtctgtt gctcccctct ttttgtccat gtgttctcat tatttagctc 317940 ctacttataa gtgagaatat gcattatcag gttttctgct tctgtgtcag tttgctaagg 318000 ataatggtct ccaattccat ccatgttcct gaaaaggaca tgatctcttt ctttgttttt 318060 atggctgcag tgtattccat ggtgtatatg taccacattt tcttcatcca gtctaccatt 318120 ggtggacatt taggttgatt ccatgtcttt actattgtga atagtgctgt aatgaacata 318180 cgcgttcatg tgtctttatg gtagaacgat atataatcct ttgagtatat acccagtagt 318240 gggattcctt ggttgaatgg tagttctgtt tttagttctt ttagttctgt gtgtgaaaac 318300 acaccagaga acaaaattga ccagatctct gccttcacag aacgttcatt ctactgtacc 318360 agttatttca tgaggaatat gaatactgtt attactctct gcccatttca cacatgaagg 318420 °agcagaaatg gagcagagtt tactaattta tctgttgcag atagtagcta attgtgtgcc aggtgctgtt ccaagcattt tacccctgtt aactcagctg agtgctttta ttttccccat 318540 tgtatgccca acttatggat gacagcactg aggtagagaa aggttaataa catagaatag 318600 tttagtatta gggaaacaat ttgaattcta ggacactttt actgaaactt agcataggct 318660 aaagaagtga tcgtgcatgt ttaaagagtt gtatcatttt atcattattt gactttcttt 318720 tccaaaaaaa acaacccttc tcctttctct ctcttccatg tgacaaatac atgtctgtat 318780 acatacatat atatataaat ataaatatat atataaatat atataaacat aaatatatat 318840 atataaatat atatatatat aatttttttt tttgagatgg agtctcactc tgttgcccag 318900 gctggagtgc agtggcatga tctcggctca ctgtaagctc cgcctcctgg gttcatgcca 318960 ttcttctgcc tcagcctccc gagtagctgg gactacaggt gcccgccacc acgcctggct 319020 aatttttttg tattttttag tagagatggg ctttcactgt gttagccagg atggtctcga 319080 tctcctgacc tcgtgatccg cccacctcgg cctcccaaag tgctgggatt acaggtgtga 319140 accaccacgt gcgacccaca tgcctgtata ttacagccgt tgaaggacac aacttttgcc 319200 attgggcgac ccagtcccct agtaagtgtt caaaaatatt cgctattatc attgttggtg 319260 atttcgagga tttggatata attaatttga gcctaatttt ttaagactat tacataacta 319320 tacaaaaaaa tcatgagaga gcctggaatt ttgcaaaact aggatagaaa ccagtttcta 319380 aaaagcattt taaacattca gcttgtgtat ttcttttcac ttgtattttg tgatctgcct 319440 ccatgctttc atgcctttct cttcaaacaa tcttattacc actaaccaaa aacaattaaa 319500 atttgattgt tttgttttgt ttttacatgt tgttacagtt aagaaaaaaa aattctaggt 319560 attgacccct gccagttttc ggagttaata ttaactagtg atttggggct acatttaagt 319620 gctttaagct atcctacaag cttaaagtag tatacatgca aatacttaaa tgaattaggt 319680 acaagaatac agaggtaaga aatagaatct attgtagaat aaatcctatt tcaaatgtga 319740 ctgttttagg atttgaatca accacatatt acagaaaacc ccaagataaa gccttggcat 319800 aaccaagaca aagtttattt ttttctctca cataaaatta gtccagactt attttaccag 319860 tggtatggca actccacagt caaagggacc caagctcatc tgaccttcga ttcccttatc 319920 ctgggacagg cttacattct caaggtcgac tcctggccta atggggctac tggagcttca 319980 gccatcacac tctgtgcttc ctgttatatc tactggtcag aacttcagat acatctgtct 320040 aaaaggaacg ctgaaaatac aatctttaag ctgtgcaatt gtacctggaa taaatgagtt 320100 cttttaataa aaagagttga atggatattt ggtgggcaaa tagcagtcca tgctacaata 320160 $g ataaacttca caattttaga attagatatc ttgtaatgaa tattctgacg tggtgtcaac 320220 ttataccata gatagattat aactttgaaa aggaattaga acacaataat aataaattca 320280 cactaaggct cttttgggga aaaaaaatct ctaccattta ttgagtgttt attatcttca 320340 caacatccct atgagttagg gactattttt gtggacattt tacagataaa gaaacagagg 320400 cattgagatg ataactagct tgactaagga gtggcggagc tctgggcagt gtggttcctg 320460 tgtgcccata aggccattgc acaatgctgc ttcatcatgt aattcagaaa gtattccagg 320520 atgtggcagt tggtcatcat gaatgttcat tgttatcttg tggctttgtg aaaaattctg 320580 gtcattcaaa cgatttgact gttgagattc tgtgcacatg agattgtact gtgtacatgt 320640 ttctaaaaat gtatgttata gatcaaaatg cacacactca tttacctcta agaacagttc 320700 ttatattgaa gggaaattat ctgtacatga gagaacaacg tggttttgag tacaagtggt 320760 gaacagacat tgaatagttg ttttcagaaa gcatcactcc cctgtacctc agaacccagc 320820 ctcagtgctg gttgcgagcc aacagctttg atgtcctgaa gtttttgcat cacttctctt 320880 ttgcccctct ttgggattca aagatgataa gtttgaagtg gagttctacc gcttccattg 320940 gaagagaaaa agtttccgtg tgtgtgtgtg tgtgtgtgcg tgtgtgtgtt tacggagagg 321000 agttctatgc atctgcagag ggtgctgcca tcaacaagga gaacagagtg ggatgaaggg 321060 tttgggaagc caggatgggc atctctgacg agctccagaa ctctccacct aagtgtgcaa 321120 gtgtaaacac~tccctgatgt gtgaactggc catctcaaga ctaagtattt agcaggaaat 321180 gccccctatt caacccttgc cttctcaggt gtagaggttg ggttgtctct tccatctttg 321240 tttgaattat gtcattaatc aaccttagtg aaagatcact tagtcatttg tgacagcata 321300 agttcttaat tgttaggaat cactggtgcg gcacacatct ttttctatcc atgaacaacg 321360 tcaaatgctt attttctcat gagcttttat tttttccttt taaaaaaagt ttcttaggat 321420 aaacacagcc tttttccttg tctctccctt gccctctcat ctttttctca atctttatat 321480 tcctatatgt cactgaagag tccccgtgcc aacgctgtgc agtgggaggc tcctacctcc 321540 accagctttt gaggaggttg tagtcctgca accttagagg ttccacagcc aagctggggg 321600 tctttctgga gcatgggtgg tgaatctgag atctatgcac ccaggaagcc tgacacatta 321660 ttgtggtgtc tcaattcttt tttttttaat tagaaaaatt gtatcaaatt gcattgggtg 321720 agagcaaaaa taaactgaag ttggttgagc tttggaagac tacaagccac tgtaatattt 321780 aagatttctt gacctccaga actaacattt gtcctgtcag agaaaataat tactcctgtt 321840 gagaatacat gcattaaagt aagatgttca ctactctata tgatcaccaa acattaaata 321900 atatgtttta cacatgtatg tactatgtgt tcaagtgttt ataaatccat ggagccaata 321960 gaatgtaagt tctatgaggg cagaaatttt aatccatttt gttcctagaa cagttcctga 322020 cacatactgg tgttaggggt aggtcttcag attttatgga tcaaatggaa tctccacact 322080 taaaaaaact tagagaaaaa ctgccttaat gtgcccatag tctggttgga aatggcagct 322140 ccaggttcat tgattgcttt gttcattcgg cacttttcac tggacatgct atgagcccag 322200 cagtgttctg ggtttttggg atacaccaag ccctgcctcc ccggtgctta acattctagt 322260 gggggagaca gaaagaaagc aaacatggtg gacaacttaa ttatctattt ttgaaagttg 322320 acaggaactg tggacaaaag aaaacaagag ctggagacag cagtgccagg gcagggtgag 322380 ggtcaagtta gagtaagcct cattgagaga tcttttgagc aacacctgaa gggggaggtg 322440 agaaagttag ccatgtggag ggaacagcat tccaggcaag tggcccacca agtgcagagt 322500 cctgacagca agagcagttc tggaatattc tagaaacagg aagaagacca atgtgactag 322560 agcagagtga ggcaggagtg agagaaaatg tgatcaggaa agtaaggtcc tgtggccact 322620 aagattttgg cttttattct gaaggaaatg aggactcatt gcaggacttt gagagcatga 322680 tctgacttgt cacaagtgtt ctctttgtct actgggttga gaagacatcc aaagggggcc 322740 aaagattgag gcagggagag cagctaggac aggctctagc aatctaggtg ataaatgagg 322800 accgatggtg gctctgacaa gggtggtaat ggagacatgg tgaggagtga ccacatttca 322860 aatatatctt gaggtagagc tgacaggatt ttcccgatgg gctgggcgta gtatgtgaga 322920 gaaagaattc agttatggat gattattttg tcctgaacaa tgataaaagt tgagtcaaca 322980 actgatatgg ggaagtctac aggtggaaca agtttttgag gaggaaatca gttcagtgtt 323040 ggctatgttg aatttgagat gtttttagac catctgagtg gagaaatctg gagtttagga 323100 gagaggctgg acttgatatg atgttatggg tcctcagcat agagatgatg aatgggatta 323160 gcaaagtagt gagcgtaaaa aggaaaggga aggagaccaa agattgagcc ttgggacagt 323220 cagaaagaag agagggaacc cgcaaaggag attgaaatgg aatccatctc actgtggcat 323280 cgtgaaagac aaatgaagat ggtataagat gaaaaagtga tcagctgtat caaatgctgc 323340 tattgggtca agtagggtga gaattgataa ttttccatct tgtcaagagc agccatgata 323400 gagggagggg gtggagatat acttggaatg agttcatgaa aaaatgggag ggaagggatt 323460 agaagcagca agtataagca gctctttcaa aggagggaga tggggaatgg ctactgggat 323520 gccatcacat agataggaag gtggaaccct gtgcactgga ggagggagtg tcctcacaga 323580 ggaggacagg caggaaggta gagtgggctg ctttgggcac atgtggttct gtggatgttc 323640 tcttttgaca gcttcagttc ttcagggaat tgggagcaag ttcatcagct gagagtgaac 323700 atggggcaga cagtgtgaga ggtcaaagcg gcaagagtag ttgtgccaca gtcttttagg 323760 agagagggaa agtaaatgga atggggaagg aaagtaaata gcatggctgc tgagctgcgt 323820 tacaagccca cccggtgttg ggttgtgagg tgtagttgtg ggctcttctt cagtcggatt 323880 gtcagcatgg gcagctggca gagagttgaa tctgacaggg cagcagttct ggaaaatgag 323940 tatgatgagt caagaaaggg accaggaagt ggaaactgtg tgtgaggcag tgatgctgat 324000 gaatgactgt ggaaaacaat ggaggtgagg agggagtgga tgttgagtgg ctaggggaca 324060 gtgaaaaggg agtaggatcc actagcttgc tgcttgtctc ttataattag gatcaagagc 324120 ttctgccatc ttctaggcat tctgctgctt caagggagtc tctgagattg ggttctgcat 324180 ggatgtattg aaacatgcac ctaggtgtgt ttggagtcct gccagagacc tgctttctct 324240 ttattcttat cctgaggccc tttaggaggc tcagatggaa taatttttat ggtttcttaa 324300 aacccaagtg ggttacctaa gccatcctag ttctgccccc tgttccctga gaggaccacg 324360 ggagcaccct gaaatgggtt ttccactctg ccatccccca cttgcctgct tgggtgttga 324420 cctcagccat ctacacatat cccggatgta gtgttgggcc cctggttgat gtcattcctt 324480 ttcaaatggg atcttttact acagcaatcc agcaacaaac taaatagcga tagagttttc 324540 tttcccttac aaaccacaac cacaaccaag ggaaaaacca aaccaaacca accaaacaaa 324600 caaaacaaaa gtatgtggct tcttaccaca gccccctatc tcgcccaagc cttgtggctc 324660 tttgatctct ggtgttctga aattgcgtaa tcatgtgctt gctctttttc attcattgtg 324720 ctgggcacac acttaatgga ccctttcaag ttggttttct tttatggggt aagggtaggc 324780 aggactattt tttaagattt cttccacttt gtcttccaat tgttctaatg agttttttaa 324840 aattgtgcct gtcctgtttt taaattccaa gagatattct ttgtcctcta aatgtttctt 324900 ataaatatat ataaaatgaa taatataagc atatagtttt gcatagattt gagttcgctg 324960 ttacagtgtc cttttatttc tctgaggata ttcattatag gttttttaaa aaacactttt 325020 tctggtcccc aaattatctc ttctctctag acttcctcca ttcatgcctt ccttcccccc 325080 tccctccctt cctttgctct tgctcttgat tttggaggct tgtcataaat atttgaccat 325140 ccattcattt tttaaccata acgaactaaa aatgttaatt ggagccctgt gtgttccttg 325200 agcagaatgt ttggattaat gagcttccgt gcaatgtttt ctctggaatg gattaatttc 325260 tccagatcta taatttttca atctctttcc agggggtaaa agcctgattg tctgtttcat 325320 gaaagtaaaa atgtgggtaa gagtgatccc agcattcaga atgcagactt agtagctgct 325380 tgttttcagt cctgggactt cacccaccct caccacagtt ggtatcccaa agtgtgaaac 325440 tccttagatt atctttctca agaaaatgaa actcagatat tcgtgtcctt tggccatttt 325500 tgattagctt atttgttttc ttccttttga attgtttgaa ttccttatgt attttgaata 325560 ttaacccctt attagattta tggtttgcaa atattttccc ccactctgtg ggttatcttt 325620 tcactttgtc aattgtttac tttgctgtgc ggaaagcttt ttagtttcat gctatcaaac 325680 taattttgct tttgttgcct gtgcattcaa ggttgtatcc aaagaactca ttgcccagat 325740 caatgtcatg gagcatttct cctatgtttt cttctgatat ttatatagtt tcagtgcttt 325800 tctttaaatt ttaatccatt ttgaatggat ttttaatagg gcataagtgt cacttccatt 325860 tttttatatg tggatatcca gttttcccaa caccatttat taaagaggct gtccgttccc 325920 tattgtgtat tcttggcact tttgttgtaa ataagatgat cttacttgtg tgggtttatt 325980 tctgggcctt ctaatctgtt tcattaattc atgtgtctgt ttttatgcca gtatcacact 326040 gctttgatta caatagcttt ataatatatc ttgaaatcag agagtttgat gcctctagct 326100 ttgttctttt tgctcaagat tgttttggtt aattggggtc ttttgtggtt ccatacagat 326160 ttaaagatta ttttttctat ttctgtgaaa aatggcattg gaaatttgat aggaagtgca 326220 ttgaatctgc agatcacttt ggatagcata gatttttttt aacaataata atttttccac 326280 tccatgaaga ggtatacttt tttcatttat ttttgttttc ttctatttct tttatctgta 326340 ttctgtagtt tttagtattc aggtgtttca cctccttggt tgaatttacc ccaagtattt 326400 tgctgttgtt gctattgtaa atggaattgt tttcttaatt tcctctttgg ataattcttt 326460 attattatat agaaaagcta cagatttttg catgtttatt ttgtatgctt caactttact 326520 gcatttgttt atcagttttt aacagttttt tgataaaagt tttggtgttt tctgtatata 326580 tgatgatgtc atcagcaaac agagacaatt tcatttccct attttccttc tttttttttt 326640 ttgagatgga gtctcactct gtcacccagg ttggagtgca gtggtgcgat ctccgctcac 326700 tgcaagctct gcctcctggg ttcacaccat tctcctacct cagcctcccg tgtagctggg 326760 actacaggtg cccgccacca cgcccagcta atttttttgt attttta~ta cagacggaat 326820 ttcactgtgt tagccaggat ggtctcgatc tcctgacctc gtgatccacc cgcctcagcc 326880 ttccaaagtg ctgggattac aggcatgagc caccgcgccc ggcccctatt ttcttaatca 326940 cgcgatatat acaggcttgt tgaaaaacat gaaaatggta aagaagttta tgtattaaga 327000 ggcaaaagct atgatgttca actcctctaa aaccacttct tagtaggtga acattgttaa 327060 acagttttgt gaatctgctt ccagattttt tctaggcatg ttcatacaca tatacacaca 327120 taatttattt ttacccagtt gcgatcatgt atcatctgat ccaaaactcg ctatttttca 327180 gttaatatgc agtgactgtc tttccaaacc atagtatata tatatatctc taagaatttt 327240 tcaaatctgc tatagttttt aaacatattt attgttacat acatatgtgt ataagttact 327300 tatttgctta tttctttgaa tatctttgtc tagctttgga atcacataaa taatgagtac 327360 aaatggtaaa caaatttcat gtggaaggat atatagaaaa agtacaagtc tctcttctca 327420 tccactctat ctcattacct aaaaggttat ccttagagct ttatataata cacccctgaa 327480 gtttagatca ttttattgcc tcccaattgt tgagagtaag gaatttagta catatacctt 327540 tcctttcact ttcctaccct ctacctactt aatgagttcg attatagttt ttcagttcac 327600 ccactgttaa ataatatacc tatatttata atatacctat ataatatacc tataataaac 327660 ctatattttt gacttatcaa ctttggacat atctattggt gacttgtgat gagagatgaa 327720 ataatttgta catttaccct tcttttccat tcttttcccc tacccattct cccctttttg 327780 gctaaatgat tgcttttagg ttgataaggt ttataatagc tactgttctg tggttaataa 327840 aatttgtttc atattttatg tgaaatttga ttatacaact agcattcaaa acattattat 327900 gtaaatatta gttactgtaa tcacacagtg acgctcaagg aggtatttac taagcataca 327960 gatcattcct tctggatcca actcagcagt cctttgctgt gccttacctc atctttcgtg 328020 gattcctttt cttttgattg caatatatcc ttgagtaatt tttcagaatc gatatgtact 328080 gtctgaactc tcttataccc aaaaaatttg ctttcacatt tgctaatagt ttgacagagt 328140 ataaaattct gctttcaaat tctttcccct tcatggcttt gaagatactg ccccactata 328200 tattctaaaa tccactgctg ctaatgagaa attctggtgt cagtctgact cttatttctt 328260 tcatatcact tggcattttc ttccgagaaa atattactta tcttcagaat gatgaacctt 328320 ctctatcatg tctctaggtg ttagggcttc tttcatttaa tcctgtaggt cctcaatagg 328380 ctttttaatt aaagtctcat tttattcggg ctctgtgaaa ttttttgtta tgttttggct 328440 tctttttctc ttttgttgtc tctcttttct ccttgaatgt ctttctgata gatatagcat 328500 tcccattatc tatcatgtct ttcagattat cctacatttt tctctttgtt ttctttttct 328560 accatgttta gaattctctt acacgtaaga catcacttac tagatcttca gtcatatcct 328620 ttttattatt cagtctgtgc atttttaatt ttcaattttg gcaaccacat ttttaatttc 328680 taagaatcta ttttgctaat agtacttttg tcctgaaact ttttatatcc tgcaatattt 328740 agaaagagca cacccttatc cacagctgat taggagtaca ctctgaatgc agggcacgat 328800 gggcgaacat tttgttactg gcctcctcaa gcctcaatgc ccagaagtta ttgcctccat 328860 cagaaaccct tcttttgttc tatctctcac ttccaaatga agttccagct taatttttgc 328920 tgtcactgta gtgggacaag caaatcccta tttcacgtga tcattgctca aacctctatt 328980 gacaatcctg ctttgcattc ccttatttag agtgggatgg aaaataaagg ctatgaaggg 329040 ggaagaatct gatcccacca ttctcctttt tcattgcagt tttctcctgt tttatggatg 329100 caataagctg ttgcaatctc tcaggatgga aaagaacatt ttaaaactac tctactgcca 329160 gaatgatctc ttttttttaa ttatactgta agttctggga tacatgtgca gaacatgcag 329220 gtttgttaca taggtatata cgtgccatgg tggtttgctg cacccaccaa cccatcattt 329280 acattaggta tttctcctaa atgctatcct tcgcctagcc cctcacccct tgacaggtcc 329340 cagtgtgtga tgtttccctc cctgtgtcca tgtgttctca ttattcaact cccccttatg 329400 agtgagaaca tgtggtgttt ggttttctct tcctgagtta gtttgctgag aatgatggtt 329460 tccaacttca tccatgtccc tgcaaaggac atgagctcat ccttttttat agctgcatag 329520 tattccattg tgtatatgtg ccacattttc tttatccagt ctgtcattga tgggcatttg 329580 ggttggttcc aagtctttgc tattgtgaat agtgccacaa taaacatacg tgttcatgtg 329640 tctttatagt agaatgattt ataatccttt gggtatatac ccagtaatgg gattgctggg 329700 tcaaatggta tttctggttc tagatccttg aagaatcacc acgctgtctt ccacaatggt 329760 tgactaattt acactcccac caacagtgta aaggcattcc tatttctcca catcctctcc 329820 agcatctgtt gtttcctgac tttttaatga ttgccattct aactggcata agatggtatg 329880 tcgttctggt tttgatttgc atttctctaa tgatcaggga tgatcagctt tttttcatat 329940 gtttgttggc ctcacaaatg tcttcttttg agaagtgtct gttcatatcc ttcgcccact 330000 ttttgatggg gttgtttttt tttcttgtaa atttgtttaa gtaccaaaaa cagatacata 330060 gactgatgga acagaacaga ggcctcagaa ataacaccac acacctacaa ccatctgatc 330120 tttgacaaac cggacaaaaa caagaaatgg ggaaaggatt ccctatttaa taaatggtgt 330180 tgggaaaact gactagccat atgcagaaaa ctgaaactgg accccttcct tacaccttac 330240 acaaaaatta actcaagatg gattaaagac ttaaatgtaa gacctaaaac cataaaaacc 330300 ctagaagaaa acctaggcag tacaattcag gacacaggca tgggcaaata cttcatgact 330360 aaaataccaa aaagcaatgg caacaaaagt caaaattgac aaatgggatc taattaaact 330420 aaagagcttt tgcacagcaa aagaaaccat catcagggtg aacaggcaac ctacagaatg 330480 ggagaaaatt tttgcaatct atccgtctga caaatggcta atatccagaa tgatctctat 330540 tttatttttt atgacatatt aatcatgttt gttctcttgt gtcctctctt tctttctctc 330600 gattttccag tggtccacgg tggtctattt gtagggttca tatttacaaa tgggagtcaa 330660 ggtgactcca ctacagttat gtgaaaaagt ttccctgcca tgcctctccc tacactgggc 330720 gagctgatct ggttccgggt cagtggatgg agcatttcct ctcgcaggca caccttcagg 330780 ctggtgggag caacttgggc catggactgg aaccatgtca atggagaaga cttcctctgt 330840 gccaggtcct ggtcagagct gccgtcttga tttatttgta tccctctttc cgtctgtggt 330900 aaagatctgg aggttcttaa gctctctctg gcctcaaaac ccacagcagg aaatttcctc 330960 actcagatca cccacatttt tagcaagcag agcttcagtt ttgagtggag ggcaggagta 331020 ttgggcagta gggaaggaca cagttctcta cgccgttctg aatgcagtgc attaataaac 331080 tctcttcaca gttgctccag ggtgctttgc attggtgaac ttctggcttc caggaagaac 331140 ctggttacct ctgatgtgtt gggttgttgt tccatctact ctaaattctg tgccaatcca 331200 tcccatctgt ttcctaccat tggtttattt ttaaaaaata ttttatattt atgtagattt 331260 atgaacttga ttattatacc atcattagag tttgggtcca gagtcaaact tctataaacc 331320 tgtaatcaaa aattggcctt tattttcatg tagaaggaag tagcagacca aaatggagtt 331380 ctatattcca tttttctaaa tgagccattg agatgtagag caagcaagtg cccttttgag 331440 ggaatgtgtg tgtgtgtgtg cacacatgca agggcatttt cccatgtctc ccatccttta 331500 attattccag tagacagtgg gccagcagca ggtggtggca taacttctcc atttgcagtg 331560 ctgtggctgt ggtgggatct gaacaggagg caaccttggc aacagtagga atagaatgtg 331620 gagtcagcag gaagcgcttt tccctcccac ttacctgtct tcttgtagca gacagcagtg 331680 cgggcagctt cggctgactt gcttagcctg tttccattgt gagcataaac accttagacg 331740 ttttttcatt tcaggacact ggagcttgat gaaattaaac tacactcatt gtctcaggca 331800 aatctcctaa tcctattctg tgcttttgtt gttgttgttg ttgttgttag tggtgtgttt 331860 ctctctccac ttcgctttcc ttttaaaaca caacaaattt ttttttttaa ttttaaatga 331920 acttattcat tgcctcccta tgtgtcccct cctttctagt gatattgccg ctgttcattc 331980 ccagctgttc ttgattcttg tacaagactc tttgctgggc ttctgccttc tagacgcttc 332040 accttcccgt tatttcttcc ctctgccccc ggaaaggccc ttaccatggc tctgatgatg 332100 atgacagctg acccttttcc aaggtggaat ccacgtttga ttctctgatc ttgtactggc 332160 ttagcacagt gctcggcatt cagtaaataa ggttaaggaa ggccagtgat atacagtagt 332220 agaaaacagc agttaatgaa ggcttgcttt ctgtcacagg ttcctctcct ccaggactgc 332280 agcatctctg tctttctgtc gtagcataca agatagcatc tctgtctttc tattgcaact 332340 tccaggtgag aagccctccc acaagcatat acaaatttta tggaaacaga caaaaatttg 332400 tagggaagca aataccttca aggttgagtc tttctggtct tctagaaggt aagcttggtg 332460 atggggcagt cacagcccta gtgcttatgg aacttggccc tttggggaca gatctcagtt 332520 aactaaagaa atgctatggc acagagtacc ttgaatctcc tctaatttat gctgtacagg 332580 gaattattca cactttattg ttaataacac attacatggg ggaacaccag acagctgacc 332640 aggactcctg tgcttgaaag cttctgctct tcagcagggc agccagcctc ttctcggttt 332700 agcacagagc ttttattctc ctcgctctct ctaaactcct gttcaagaca gtttccaatt 332760 ctgttgaatt cactcattta cctcatctgt gcctttcccc acctcattct ctccccttca 332820 gagtgcccat ttctgtgttt caaatcccac aaggccaagc ttagatgcta ttatgtctat 332880 gacattctcc acctctatcc caataaaaac ctttgatttc atcttgctgt ttgtatcttt 332940 actctggtat atttcaccca taccagagtt gtttgtggac atgcctgatt tccttcaggc 333000 tgtaggcata tttgattcat ttttgtactc catgagtgcc taacatagca tcttatacac 333060 catagataat taatagatgc ctcttggctg catttgtatt aaatttttac catgtacttg 333120 tcctagctag cagagacttg gggggaaata atgatgactg atttttactt atttatttat 333180 tttttattcc cataggtttt tgaggaacag gtggtgtttg gttacatgaa taagttcttt 333240 agtggtgatt tctgagattt tggtgcatcc atcacacgag cggtgtacac tgtatctagt 333300 ttgtaatttt tttatccctc actcctttcc ctccctttct cccgagtccc caaagtccat 333360 tgtatcattc ttatgccttt acatacccat agcttagctc ccacttatga gtgagaatat 333420 atgatgtatg gtttttcatt cccaagttac ttcactttga atagtggtct ccaattccat 333480 ccagaaccct caaaaccatg caaaaccatg gaaacaaaac agcctgctcc tgaatgatca 333540 ttaagtcaac aatgaaatca agagagaaat ttaaaaattc tttgagctga atgataatag 333600 cgatacaact taccaaaacc tctgc~gatac agcaaaagca gtgtcaagag gaaagttcat 333660 agcattaaat acctacatca aaaagtctga aagagcacaa atagacaatc taaggtcaca 333720 cctcagagaa ctagggaaac aagaacaaat caaacccaaa cccagcagaa ggaaagaaat 333780 aacgaagatc agagcagaac taaatgaaat tgaaacaaac aacaacaata aaaaagataa 333840 atgaaacaaa aggctgtttt tttgaaaaga taaataaaat ggatataaca ctagtgagat 333900 taaccaggaa gagagaggat ccaaataagc tcaattagaa acgaaacaga agctattata 333960 atcaatacca cagaaataca aaagatatta gaagctacta tgaacatctt tacacacata 334020 aactagaaaa cttaaaggag atggataaat tcctggaaat atacaaccct cctaggttaa 334080 gccagaaaga attagaaact ctaaacagac caatagcaag cagcgagatt gaaatgataa 334140 taaaaaaaat tgccaacaaa aaaagtccag gaccacacag attcacagct aaattgtatc 334200 agacattcaa agaaaaattg ataccaatcc tactgaaact attccacaag acagagaaag 334260 agggaatcct ccctaaatca ttttatgaag ccagtatcac cctaatacca aaaccaggaa 334320 aggacataac aaaaaagaaa actacagccc aatatccctg ataaatatag atgcaaaaat 334380 ccttaacaaa atactagcta agtgaatcca acagcatatc aaaaagataa tcaactatga 334440 tcaggtgggt ttcataccag ggatgcaggg atggtttaac attcacaagt caataaatgt 334500 gatacaccac atgaatagaa ttaaaaacaa aaatcacatg agcatctcaa tagatgcaga 334560 aaaagcattt gacaaaacct agcatccaat tatgacaatt tttaaatatg gggaatggtc 334620 ttcatggaaa agtagatgtt aatggcacct gtattagggt tctctagagg gacagaacta 334680 ataggataga tgtatatata aaggggagtt tattaagaag tattgactca cacgatcaca 334740 aggttgggtt ccacaatagg ccgtctgcaa gctgaagagc aaggaagcca gtccaagtcc 334800 caaaacctca aaagcaggga agccaaaagt gcagccttca gtctgtggtt gaaggtataa 334860 gagtcccaaa gctgaagaac ttcacgtcag aaattcgagg gcaggaagca tccagcatgg 334920 gagaaagatg taggccagaa gactaaacca gtctagtctt tccatgttct tctgcctgct 334980 tttattctgg ccatgctggc agctgattag atggtgccca cccagattga gggcgggtct 335040 gcctttccca gctcactgac tcaaatgtta atctcctttg gcaacatcct cacagataca 335100 cccaggagca atactttgca tccttcaatc caatcaagtt gacattacat attaatcatc 335160 acagcaacca aaagattatc tcatttaatc cttacaatag ctctgtgtag tgggtatata 335220 ttttcctttt gctgaagagg aagtaggctt agaggggttg agtaatttgc ccagaatacc 335280 taggtagtag aagatagtag agccattata ttctgtctgc attcaaaaga gtgtgctgct 335340 ttatttgttc ctgaattgca cacaagctaa agaatacaac tggatgttca gtctactcct 335400 attttgataa cttggctaac tttactgcag agtctgcaga ggtgaccaat tttactctgg 335460 gagataccag ggaatccgcc atgtaattcc tcaggctgag tgttttggaa acacaaagct 335520 gtcactgcta ttaagtgatg ttttttcctg agagtctact atgctcccag taccagcctc 335580 cgcaggccct ctgtctaccc cttttctgtc ctgatgtgtt ctagcaggca ctgagaaagt 335640 cattgtagaa gttaccatga ctttccaaat gcttccagaa gcagaacaca atttcacagg 335700 agggccaata agtatgaatg ccaccttgat agaaagtgaa gttttgcgtc ctgttgaaca 335760 ctcagtaaat gcttcctgaa tagatgcagg attggcaaaa catagctccc cagctttcat 335820 aattcagact cagaggccct tcgtgctcct gttattcttg ttacttcatg tcaggtacct 335880 aaaagggttg tttttcagtt ttcgtcattg atttaaagca gaggttagta aactttttct 335940 gtaaaggcca ggtagttagt attttagcct ttgtgagtat ttggtctcac aaaagcagcc 336000 atagatgata tgtaaatgaa tgtgcatggc tgtgatctga taaaacttta tttaaaaatg 336060 aggggtcatc tgggctgtgg tttttcaacc cctgatttaa aacaaaaaca aaatgtgcta 336120 tgcctgttag atgctactac atttgattct gtgttttcca tatttttcta aaatgtactg 336180 aacaggaaat gaacaaactg atgacacaat gatctaattt aatcattgag ataagagtgg 336240 cgccaactct tattgagtat ttgttatgtg tcaggcactt ggctaagcac tttacatgtt 336300 ttctatgatt tataaagcaa atattgttat tatcttcatt tttttgctga gggaaggaat 336360 aaagtcacag ccagcttaca gaggctgccg gaggtcacac atcaccagta cactgaaaca 336420 cgtggtctga ttacaggatc catgatctcc tgtacagatc atagtgatat tgacctgaga 336480 ggaaagagcc taggacttac aatcagaaat ctgggttcac atttcaactc cgttccatgc 336540 caaattacat taaaactggg cctcaccttt cccacctgtg aaagaggaat aatactagga 336600 cttgccctcc caaccttaga gattgctgtg aggataaaag cctatatgaa tgtgctttat 336660 aaactataaa caccatacaa atgtttgtta taagtctgcc atttgaatac atcttagtct 336720 ttttttccta tgtgaaataa aacattttaa gacaagcaat gaaattcttg aaactataac 336780 aggtttttaa gggtgttgat ccccaaagcc gtcttcacta ttttgctccc tctctgactc 336840 gctctgtctc tgcctagcag cgctagtctt ttctacgtgt ttgatctgtt gttcctgtgt 336900 ttaggcccat ccctactaca cagcggaagt cagctatttt ttctctttcc tttgctctgc 336960 tgagcaagat taaactcttt tcacctggac ttttcctaga ttactcaggc tgggtggaaa 337020 tggggtagct gtgaggctgg gatgttactg tacaattcaa atttatgatc tgaacttaac 337080 tagatcttgc tgaagattga cctggctagc acagttttga ggcacctatc tttcagtccc 337140 ttctgtcctc ttggcctggc cactggccac atgttagtag cttacttcta aggatggaat 337200 gccatgcatc tcctacaagg ttgtctctat gcttcacaca aagtaaaaga atggaggcaa 337260 ctttgaatgc tgctcatttt cactcaggca atccctttac aatattgtgc ttagtaaact 337320 ccccccactc tttttttccc ttagcttgtt ttttttttct aataaaagtc tatatcaatt 337380 tgatgtatta gttagggttc tctggagaaa cagaaccagt aggatcaatt gattggtcaa 337440 tcaatcaatc aagacaaaaa gagattcatc tggaaggagt tgattcatgc cattgcggtc 337500 attggctagt ctgaaatctg taaggcaggc tggctgactg gaagctcagg cgggatttct 337560 ctgctgcagt ctcaaggcag aattccgtcc tcttggggaa acctcagtct ttgctcttaa 337620 agctttcaat tgattggatg aggcctacac acattatgga gaataatctg ttttacttaa 337680 agtctactga ttgtaaacat taattatatt taaaaagtac tttcacagca acatttagac 337740 tagtgtttga ccaacaactg ggcaccacgg tcaagccaag ttggcatata gaattagcca 337800 gcaaacttgg tattcacaat ctgtcattat agtcttgtct ataaacctaa ggtttagaga 337860 aacgctggtc attctgaaca attccctgtg aatgcagaga tcaaggagcc tccccttaac 337920 tgggtaggca gtgtgaaata cctgcagtgt gcttgacctt actgttcacc cactggcagc 337980 tgttcctaca gcttggcttc agatatataa tattacattg gctaacttca aagagcattg 338040 ttttccaagg atagagatgg tgatggtgta tattactttt accagcccca gacctgtaag 338100 aggttctcat ctcttttgtt,taagattttg ttgttttttt ttaatgctca ataaaagaag 338160 ctttattctt tcattaaaaa acaaatctca gaagaacagg gaagaagagg gtagcctatg 338220 aaagtgtgat tgttatttga taccatcgtg acttagcttg ttttcaccta agactctgag 338280 attctattct gtttatgtga gatttgggct gagttcaact actatccata ttaactgaat 338340 cataaaacaa ttaatccatc atctggttac attttggtgg ggagggagca tttaacacaa 338400 cactaagcca catatttcac tatttttttt ttttgcagca aattgactta actgcttgct 338460 ttcacaccat gcctataaaa cctttttggt tttagaggtt ctatgtgtct ggaaagtaca 338520 gtgaagtatc tcatcagaat agtattaata tttcttatga catttcatat gtcctgatat 338580 gggttaatgg agaaatatca taagagtagt aattctcttg ctgttattat ttgtcctatt 338640 tagacaaaca gaacatagaa ttaataagaa ttcaaaaact ttaatcactg atagagatta 338700 aatgatgttt atattagtca tcatcaccta atatcttaaa tatttaacct tttatgcagt 338760 gtttgcctct ctacttgggt atgcccttaa cagtaaaaag tgttgataat gctctccctt 338820 ttggcactca cagaattgct atacatatat atgtataaaa tatatgtaaa tataaaaata 338880 catagttaaa aaaataaatc taccactaaa tatagtaagg gagttttaaa ctgaggtttt 338940 tgaagtcttt aagaatttat ttagagactt cttttgttgg aagtatggtg gctagatgcc 339000 atgaaaaacc cactgaaaac aagtgtaagt gctaggtaat atgtt.gaaac atatcttttg 339060 aaatgtatta ctgagctggc aagaaagtat gccattgtag agagtgaaaa taagtgaagg 339120 cagaatcctg ggaggtaagc cagcctgaag atattgatga cactggatgg ccttaagttt 339180 ccattttgac tggcatgtaa tccagccaaa gattcccaca gaaatccgag gttccaaatg 339240 gtaaatccgt ggtgacattg gggtgaccga gaaatgaact ctgtgtagaa aggaatggta 339300 acaacacttg tctcgtacaa ctttggctct gggtagggga aaggaaaaca gcttcctaga 339360 gaaatgttaa ccacaagcta gccctcatga gagatttcag ctaggatgga tgctatctgt 339420 gtagtgcaaa aaaaaaaaaa aaaaaatcca tagccagatt ttgtgcttta atgtggacct 339480 aggtcaatag tgactgcaaa gccagaagca gtggctcacc cctgtaatcc cagcactttg 339540 ggaggccgaa ggaggtggat cgcttgaggt caggagttcg agaccagcct ggccaacatg 339600 gtgaaaccct atatctacta aaaatacaaa aatgagccta gagtggtggt gggtgcctgt 339660 attcccagct acttgggagg ctgaggcaca agaattgctt gaacccagga ggcggaggtt 339720 gcagtgagcc aaaatcgcac cactgcattc tagcctgggc aacagagtga gacaccatct 339780 caaaaataat aataataaaa aaaagtgact gcaggggact gggaagagga aacacaaatc 339840 tttactgggg aatggaattt caagtgtatg caccaaccct tagaaatcac aaaacatctg 339900 acccttctga aaataaccac catgagtgag acagcaggaa aaaaaaaaag aaaaaaaaaa 339960 aaaaacaagg aaagagaggg acaatagaat cagactcata cctacaaaac ttaaaaatta 340020 aataaaatga atcagactca gaggcttcag agtttgattt aaatacatta tacaaaagtt 340080 ctgtgtgaaa tatgtttaag atgtagaagg gtgcttgaaa tgcacaaagt caacagctga 340140 aaaattgacc aagcagatat gaaaataaaa tccaatagag ggcatctgga aataaaaaag 340200 tataataatt atagtgaaaa aagttattag atgggttaat tagtagaatg gatatagcag 340260 aagaaagatt tagtcaactg aaaaatatat ccaaagaata ggtccagaat taagcttagg 340320 gagataaaga gatggtcaat acgaacatta ggagacatgg agaacagtgt acgaaggtgt 340380 aataaattct aactggagtt aaagaggaga tagtaaagag gaagaaaaaa cacaaaactc 340440 aaaaaatgta gtggctgaga atcttccaga agtattgaaa gacaccaatc cacagattca 340500 gaaacctaca atttgcaaga aaaagtaaaa agaatttcac accaagatcc atcttcatgg 340560 actggcagag cataagagat gaagagctta tcttgaaaat gcagccagag agaaaaggtc 340620 tatcgcagtt aaaggaagag gcaagcagat gactgagttc tcaatgccaa ctgaaaatga 340680 gaagccagtg gcacaccttc aatatgttgg gagaatataa ttttcaactt aaaatagtgt 340740 acattgtaaa attacatttc aaaaaaagag ggtagtataa tgacattatc aaataaaaac 340800 cattccttat aacaaatatc actaaagaaa ctgttggagg atgtagttta ggtttctaga 340860 aaggatgtct gatatgcaag aaagaatggt aagtcaaata ttgagaaata tgtgaggaaa 340920 tagaaacaag aataactaat gaatcaatga tactgtctaa tttgaaggtt aaatgattag 340980 gtagaactaa aatatgaaac aataatagta tgtaagtcgg gaggaggagg atgaagcttg 341040 aagtgctcta tgcatcttgc atctctggtg tctttcttgt gtccaaactt tttcttcttt 341100 caaggacacc agttagacta gattagggtc cacctaatga cctcatttta acttatttgc 341160 ctctttaaag gttctatctc caaatacggt cccattttga ggcactaggg gttagggttt 341220 caacgtataa attttggggg gacacattcc agccgatagc aattataaat acaactttta 341280 tagtaaaagc ccatcttcta gaacaggtag agaacaaggt tacttatttt ttttaagaac 341340 aaaattggtt taaattatcc taaaatgatt ttaggagaaa atggacatta aatatacaac 341400 atttcctcct ttattatttt attgatgact tgatatgttt tttaaaactc agtaaaataa 341460 atgattagtc tcataatcat aagagagtta tgcccttatt ttaatgccat aaaactgtga 341520 cttctgaaga aatacaatca tataaaatca tattttaatt tacttttgag aatgtacagt 341580 aactcaggaa ggcagagtgt ggaacctgct ttaatgaata gataagaatg actagcaatt 341640 aaaaattaat tatctataat tcttctgaag ttcttgaaaa taatctcttc taacctgggt 341700 aaatattcct ccttttcaga aatgtttgca atgttgcttt tttttaaatt aatctctttt 341760 tcttagataa tggaacataa atcttaggca catttgcttt gtaaatacca aattgcacac 341820 agattaaaat ttaatgagct tttactgctg taatctcaac aagcagagtc tttgctgttg 341880 gtttttcaat aagaaatact ttattttgtt atacagtggt cattaagaac cttaaagata 341940 atcaggacag attttccaaa ttgcataatg ctattgccta gctatttgca tgtatttttt 342000 ttaaatgaca agacatttcg ttaaatattt acttgcctga cgttgaaaga tttagacaaa 342060 caatgtactt ctagttgtca ctttttcaaa aagattctta gggaaaagtt agccgttttg 342120 ttggttggta agtgatgggt tttcttggaa cgtgttgctt gctaatttta ccagagaaga 342180 aaataaattc aaatggtaag ctacattcca atttgtattc ttcttctaaa gtcctccctg 342240 tttgcattct cggattttga cactcctaca caccagttaa agcacttgtt agagtgtcca 342300 aaaaattgtt gcaattaatt tcccagtctc ttctctcttt gatgtgtcac tataatgctt 342360 tgagtaattt tcagtgtctg gcatctgttc aaggacaatt tctggatttt aggaggggga 342420 gaagcaataa aggaaacaac atatgttttt tcctaaatag gaggcatttt ttgtttgttt 342480 gtttgaacaa aaatcgaaaa ctttcttttc tatataggtg acaaaaagat acacgactca 342540 ttggttgtta actaccattg cattcttttc tctttggagt agcccttaag gaccagatta 342600 gaccagccat ctgcatcatg tagcatgtct gggttacctt tcccaaaaga agttcagata 342660 gatgaacttt gcaaaccaga gccatctgaa gtttgttttt tctttttgag acagagtctc 342720 attctgttgc ccaggctgga gtgctgtggt gcaatctcag ctcaatgcaa cctccgcctc 342780 ccgggttcaa gcgattctcc tgcctcaacc tcccaagcag ctgggactac aggcacgtgc 342840 caccacgtcc agctaatttt tgtattttta gtagagacag ggtttcagca tattggccag 342900 gctggtctca aactcctgac cttgtggtcc acccgttttg gcctctcaaa gtgctgggat 342960 tataggcata agccaccacg cccagtccat ctgaagtttt aaaatggctg caggtatctt 343020 agtaggagac taattttatt ttccaatgat tagaaaaaaa tgaccagacc aagaactcta 343080 gggttatgtt tcatctcata tcctgtgcag gatgttctga gaagtttcta tttgtatctg 343140 tggagtggtt ctagtcttgt tcatcttgca ttttaaggtt tggaggtatt ttatcttgcc 343200 ttgcttcttt accaccttgc tttaatttcc ccctatattg ccatttctaa agtaatgact 343260 cattttcaag ggtgggtctg aaaggaaaga aataataaaa ccagagatct ggagcagttt 343320 ctcgttcatg gtcttattca ctttttaata agagcatcta gaacagtgag tggtgcccca 343380 gcagatatct ggaaattttt gtggaattga attgaatttg ttcttagaat aaatgacaat 343440 aaagaaaata gtattatgga aactttcgac ccaagggaaa tatcaaggct aaagttgcta 343500 tattcttttt cttctgatgg atgctgagtt tttctcctta aaaaacaaat ccacatttaa 343560 acataaggca ttgtttgatg gcctatcaca ttcagtagaa tggatttgag aaatacgtgt 343620 aggatgccta ttaaaaactc tgttttgaaa ttaaacctaa gactataaga ttgtctgttt 343680 aaaaaaatta ttgctgctgt aatttgagca aatctgaact atattgaact ttagaaattc 343740 aaatgtatgc aatcttgtaa tataacttac tccatcggta gacatagttc tttgtcccac 343800 ctggcttcca gcagtttgtc tgtctgtcat taggctaaac tcttcatgtt cctaaacata 343860 ttttcctcaa aaggggacat atttcttggg tcagatgtaa acatcaagct aaggagttct 343920 gctgtgcgtc tagacaaata aggttcattt tcatcctcta catagtgtta aacttgaatt 343980 taagatcaca cagattcctt tacacgttac caatgtaatg atatagacaa tctcacttta 344040 ttcattcact ctgtttaatt cattcaataa aaacgtatac attggctgcc tactgtgtgc 344100 cagttggtga agatataaag atgattaagg caagggtctg tgctgacatg cctaatgggc 344160 aggctgacac acctaaacgc cagggaaaaa gagggatgag acctgggcct ctgaagccct 344220 ggccacctgg ctcgaagctc actttaatgt gcttctgggt gtattccatg actttttaaa 344280 ggcgcttatg tttcctttct ggtaaagcat gtttaacatg caaaatgctt atttcattga 344340 gtagctttta aaaagtgttc atagaatgtc tagaacagtg cttgtctaat agaagtataa 344400 ttcaggtcac gtaggtaata ttaaattttc tagtagccac attaaaaagt aaaaagcaac 344460 aggcaaagtt aatatttttt tttttcctga gatggagtct tgctttgtca cccaggctgg 344520 aatgcagtgg tgtgatctcg gctcactgca agctccgcct cccgggttca agccagtctc 344580 ctgcctcagc ctcctgagta gctgggacta taggcgcccg ccaccacacc tggctaattt 344640 tttgtatttt tagtagagac ggggtttcac cgtgttatcc aggatggtcg ttaattttaa 344700 taatatattc attaatttta ataatatatt catttagtcc tatataaaaa aattagcatt 344760 tcaacatgta atcaatagaa tatattatta gtcaactatt ttgcattctt tgttttgtac 344820 catctttgaa tttcaatttg tattttatac ttacagtgca tctcaatttt aatgctaaat 344880 gtcatcggag atattcgatc tctgtttagc tttaatatat ttttgggttg aaaaagtaaa 344940 ttcacataac caagttcttc caaatatact tgaaagtgtt tctgataact gagttatcaa 345000 cttaaaaatg taagttaatg acaataaatg aaagaaaaat tctgttcctt agctgcactg 345060 accacatttt agtgttcaat agtcgcaggt ggccagtggc tatcatattg gatagtacag 345120 ctttagaatg acagttcagt gcaacaagtg ctataataga ctcatgaact ggaagctccg 345180 ggaagttaag aatgggatca atgaagtggg tcttgaaaaa ccaataaaaa ttcaccacgt 345240 agagaggagg agggcgacta ctccaattca atcttttgaa agcatgtgaa ggagcaacag 345300 tagacttctt taattattta atagtaattt attgaacatg aattatggga ggtgcatggt 345360 gtgggtgctg gtgagacata gttcctgagc tcaagtagct tggtgtctaa atattcatgg 345420 gcccattttt cagaaaggat ggatatgtgt gtgatcctgg gtgtgccaaa tgctgtggct 345480 tcctgaagct tagatttcca gcttgtcacc ttcaaggtta ccttgtgaat aggacttttt 345540 tgagctgtaa gtaaatttac tttgcctatt tatttccaat ggaaaaaaag cttttttaaa 345600 aaataaatct catcttattg cctatgattg gccaagacaa catggcccat acagaaggtt 345660 tttgatggct tctgaggtct gttatcattt gcttattggc atttcagctg tcaaccaggg 345720 ttctgtcaaa ttccattcct gcctttagct cttttacttg aatacctggg gcaatggcag 345780 aagtggttgc tatttgtcac ctttccagga tgttagtcgt gtccttgaga caaatagaaa 345840 atttaaagtc agatgacttg attcctctgc cagttaagac ccttagagag tcctcagcat 345900 gttgcttatt tttaaatttc aagtccctgg taaggatcaa gtaaactccc caatttgcag 345960 atttctatcc agttgactat ggattttgcc tgttgctttg tttccaccaa ctctccctga 346020 agatgaggcg cacagacaga caactcacag gcaagaacag cctggtccat cttgaaagat 346080 tctcaagact attctccaca agataattgt ctacttttaa aaatattcag taaagggaat 346140 ttttgctgtt atccttggtt gtgtttttag tatctcatca tccttctagt tgtaggaggc 346200 ttttcctaac atctaaccca tatgtctgtt gtctcatcag gtgtttctat taaggctact 346260 tccccatcaa tcttaatttt ttttttaatc ttctgagatg tataggttaa gttgaaatca 346320 gagactttca taaaatggta agatggccat ttaaatgcaa ctatgaggaa ataatgtaag 346380 caggattcca ttggagaacc aaacactagc aacaactaac ttggtaatca atgttgggac 346440 ttgaagttag gctaaaatca atagtaatgg cactcgtatg tacaaatgca gaacttttta 346500 cacacaatga tttttccctc tgttattata cactagctgt gtctgagtag acagtccagc 346560 tccactacct gcagtccacc ctgggctgtg taatctgagc actgatgggc tgttatttgt 346620 acgtattact tctatgacac tgttttttca tctgtgttag aaatgtcttt ctttcttgaa 346680 taagctgctt caatttctta tagacagatt ctgcttttat gacccttgtt tctacaaagt 346740 aatctctact catgctgaaa tctcagacaa ttttaacaaa tatttagagt acttaatttt 346800 tctttgaatt ctaaatatta tccttcttta gttcaccata gttggatatt ttgagataac 346860 tgtggaggaa acagcactga tcctggggcc atgaaacctg atttcaagat ctagtccctt 346920 ctttaatttt gcacaactaa tttaatcaca ttcatcctta atttcatcat cattaaaata 346980 gcagagaata attcccgttt catggagttg atatgcaatg aaatagcata tcaatgtatg 347040 tatgaatgtc cccccacata gagtactaca caaatgaaac gtgtcactca gcataacgtt 347100 atgtgctcct tcctgcaact ggattgcact ccagtgggat ttcatgctgg tgagatggct 347160 gtgctgctgg acttcatggg acaccaatct ttgaaaataa gcctgatctg gctgggcgtg 347220 gtggcttatg cctgtaatcc cagcactgtg ggaggctgag acgggcggat cacctgaagt 347280 caggagtttg agaccagcct ggccaacatg gtgaaatcct atctctacta aaaatacaaa 347340 aaaattagct gggcatggtg gcatgtgcct gtaatcccag ctacttggga ggctgaggca 347400 ggagaatcac ttgaacccag gagatgaaga ctgtagtgag ccgagattgc gccactgcac 347460 tctagctggg gcaacagagt gagactctgt ctcaaaaaaa aaaaaaaaaa aaatagcctc 347520 taacaaaaaa aaaaaagaaa aagaaaataa acctgatcta tagatgaggc cagtggatct 347580 tgtgaagagt tgaaaggtca ggtatcagtc ttataccatg tcggatgggg caatcgtcat 347640 aactttttaa aaaattattt atttattttg agacagtgtc tcacgctgtc ccccaggctg 347700 gagtgcagtg gcatgttttc ggctcactgc aacctccgcc tcctgcgttc aagcgattct 347760 catgcctcgg cctccctagt aactggatta caggtgtgtg ccaccatccc tggctaattt 347820 tttgtatttt tagtatagac agggtttcac catgttggcc caggctggtc tcgaactcct 347880 gacctcaggt gatccacctg cctcagcctc ccaaagtgct gggattacag gtgtgagcca 347940 ccgtgcctgc cccaattgtt acagcttttt aagtggatta tatgcctgct tcaggcatta 348000 gacataataa gttaatctgt tgataagttt cttcatttct taattttcag ttgtgttttg 348060 atgtgtgtgg tctgggtgag cggctcagtg ctgctgctgt ggagaaggca tgatatggaa 348120 gggatgtgac tgtccttctt catcttcata acggggccag gactggggtg aggtgtgtga 348180 gatgaatgaa gcactggttt tgatacaaaa tttaagaaaa atcaaattca agaggtaata 348240 ttttaatacg atatttgtaa aaaatcaaaa ttagtgcaaa aatttgtgat gagcaaaatg 348300 ttagataaag gcattgttgc ttttcttttt ctgtgtattg taacacacca tcacttagtg 348360 gagaaacagc tctcactttc ctcctcccca gatacaacca tttatgttga attaaacttc 348420 tagttctcct atacctgtga gcatccccca ggccccttgg tatggagaaa gcagtttatt 348480 tgtcttagta agattataaa agtggcaata aaatgtgaat gtaatgaaca aaatgcacat 348540 tttgtggacc aactcgttgt tttagatcta tttttgaaaa ctcattaggt atgtgcatga 348600 ggcaaaataa ttcaagtgca gttggatatt aaatgaaaaa gtgtcaggtc cccttttctg 348660 ccatattctc ttctgtgtct tcattagtgg aaaccatttt gaccagttct tataattcta 348720 atgatttgct gatggctcta attcttggtt tatcaacttc aagcagaatg tcttgacatc 348780 ttggtatgaa agttaaagaa tatgaatcat ttgttctctc ccttctcctt tcctctttct 348840 ctgcctctca gtttttgttg tatgctcacg gagtatgtgt gtgagcatgt gtgtgtgtgt 348900 gtgtgcacgc gagcacgtgt gtgtgtgtgc gcgcccatgg gtccattttt gcatggcagg 348960 ctttgtttta gaaagattgg ttacaacagc tgccccacca atctcctcta cagggaagga 349020 ttcctactct gagaccttgg tcttttccag gcagatcact tacatttcct cacagattat 349080 ttctcatcct gcagcctggg tccaaatggg ctttgctgtg aacagtgact ctcaggcttc 349140 tggtctctgc tgtgcttgag tgtcgttgag atgctccatc tcctcctact gcacccaccc 349200 agcaatcatc tctctttcag gaattcacca aagtcttttt taaaattatt cttttgtttt 349260 gagacagagt cttgctctgt tgcccaggct ggagtacagt ggcgcaatct ccgctcgctg 349320 caacctctgc ctcctgggtt caagtgattc tcctgcctca gcctcctgac tggcagggac 349380 tacaggcatg tgtcaccaca cccagctaat ttttgtattt ttagtagaga tggtgtttca 349440 ccatattggc caggctggtc tcaaactcct gacctcaggt gatctgccgg ggtccatttt 349500 acgtggcaga ctttatttta gaaagatagg ttacgacagt tgctccaccc atcccccctg 349560 cagggaagga gtcctactct gagaccttgg tcttttccag gcagatcact tacctttcct 349620 cacagattat ttctcatcct gcagcctggg tccaaagtgc tgggattaca agcgtaagcc 349680 accacatccg gctcaccaaa gtctttgggt ggttgatgtc atatgcctcc agttgatagc 349740 acttttaaga atttttcctt tttgtatgct gttattattt ttagaaggct tttggattat 349800 tattgttaaa gagtatgcca tgtctatctt ttaactttta agcatctatt gctttgttac 349860 ttgtacttta tatatatgta tatccttccc ccaataagac aaagagtgca aataatcacc 349920 tcccttgcct gttgtttggg tttaattcag gagtcagtgg aattaaaagc ctaaactcag 349980 tatagtttta aagcagcagt ccccaaccct tttggcacca gggactggct ttgtggaaga 350040 cagtttttcc atggaccagt gtgggggctg gaaggtgatt ccaggatgat tcaggtacat 350100 tacattaatt gtgcacttta tttctattat tattacattg caatacataa ggaagtaatt 350160 atacaactta ccataacata gaatcaatgg gagccttgag cttcttttcc tgcaactaga 350220 cggtcccatc tgggggtgat gggagacagt gacagattat caggcattag attatcataa 350280 ggagtgcaca acctagatcc cttgtgtgca cagttcacag taggattcgt gctcctatga 350340 gaatctaatg ctgccactga tctgacagga ggtggagctc aggcagtaat gcgagcaatg 350400 ggagtggctg taaatacaga tgaagcttca cttgcacgtt cactgctcac ctcctgttgg 350460 gcggcccagt tcctaaccag ggttggggac ccctgcttta aagaatatgt ttgtggattc 350520 atagagggaa acaacacaca ctggggcctt tcggagggtg gagggtggga ggaggcagat 350580 gatcagaaaa aatattaata actaatgtgt actaggctta atatctgggt gttgaaataa 350640 tctatacaac aaacccccat gacacaagtt tacctatgtg agaaacctgc acgtgtactc 350700 cagagcttaa aataaatgtt aaaaaattcc ctccaaaaag gatatatttg catcggagtt 350760 atatttgtat atgagtatat atttgtatat ataaatatac ttgtatatga aaaaaatatc 350820 ttttttcttt cattttattt tccaaacatt aaagtcgggc acattggtta ggaatttcac 350880 caatactttt tacaaaactg aggacttgga tgataaaggc actttttaaa agattacaca 350940 ctggcatggg gaataatacc tttataaaga tgatctatgt gttcctgaat atcccgatgc 351000 atctcctagc tggatcttcc gctccacaaa aattcataag atgagattct tgaattagaa 351060 tgcatgcctt aatgtattaa gtctatgtag agagagttat ttagtccatg taaattaagt 351120 gggaatattt ttatttgatt actgttttat ctggatcttg cccacttaag gccttcaaaa 351180 atgaaattta aggcctgtta agcagaccca acatcaacag catattctct ctctcttgta 351240 agtattgtct agttgataaa aaatttcaaa aacatgtctt aatcaaaaac aagatgatcc 351300 agcacaggtt taataaatgt tttgtgaata tggcacattc gtgtctcatt actacagttt 351360 tcctatgctg tctttctcaa taattccccc caaaattgta gtgtttacat tatggcattt 351420 atagttacct ctgaacctaa aaaattaact tcaaaagtat ttaaaaaaat cattatattt 351480 aaaaccattt catgtttaaa tggtttgtac agggcaatga aaggaaatgt agtaataaac 351540 acagaatcag atttggtcac taatattttt ctgcagttga aatatatgta gactggctta 351600 gggtctaaat agcattgaa't cctgttacct tccatctata atcaattaat gtattatgac 351660 attgttgtcc tcagatcact gggatctcat ggtaagtaag taaagggatt attctgtgca 351720 ttttcccaat atctatatta gttttaatat acctttatgt taatgtatga cactgacact 351780 tagtaattgg attaacttcc tatcagaatt tgtttttcac tcatactttg tatacatgtc 351840 ttaagggtag ggtagatgta catttttttc tgtgttagcc tatctgtttc tgtgacacta 351900 catgctttct gtcctccaat ttgtgtttct ttccgtgtac aaatatgcat catctaccat 351960 ctacatctac aaaacatgta ggcttccaat gttttcatgt aaatacattt catgagtcct 352020 cagtagagtg ttagatgagt ggttaattag taatgttaat aaatgtaaat tacaataaaa 352080 acatttataa tgttaataaa tataagttta ttgaacttaa ataatttaaa catcttctga 352140 aatgtagtag aaattatgat tagccataac tagtacagta ttttttgctt gtttaatttt 352200 tttggtgata ttccatttgt ggaatgagtg tgtgtgtgtg tgtgcagtac agacagaaag 352260 gagagaaaca tatctgcatg ttatgttaga aagagtagtg ttagctaccc taccaaattt 352320 acatactggc tgaacacagt atgtaaaaaa ttattttttt agtccattgc tggtgttcct 352380 tgttgttggg tgactctatt ccacatggtg atgcagggaa ccaatctcct tccttctgtg 352440 gctgtgtaat cccttgcaac cttgaagtcc tctgcatcta actttaggat gaggaaagtg 352500 aaggtggagg atatagccct cttgactgcc ttgacctgga cctggaacat ctcttttgct 352560 cccattccat ggggaagaac tagtgacatg gtggcccata gctgcagagg ggaggctgag 352620 aaatgcagcc tctggataag aagcctactc ccagtggtta ctgtacattg tgggagggaa 352680 gcttgaattc tgatggacag tgagaaccat tctccacaca ataacgaaat gacgtcttct 352740 caaccttggc aatgtagcat cctgtgactt aagtatgtaa ataattatca gtacaggtca 352800 gtgaaaaatt aggtcacctt tcccttccac ttttaattct atagtttaat ttgcctatct 352860 gctacatatt atatatatgg gaatagataa gattatctat aaacatgtat aaatttgttt 352920 cctacactaa aaagaaaaat gacaaagaag aattaagtta gttgcccaaa tccacacaat 352980 accaccagga gaaagtctag gaaccatacc ttctaaatcc aggtctacac ttctttagtg 353040 cactaaattt tttcctaata tagctctgag gagactggtg tttaagagga gaatgttaag 353100 ccaaaagccc atttcacttg gctctgtaca gacagtaagt taatttccag gatgtataac 353160 tcctgatttt ctgtgatgac agagaaaata ctgacctgat ttgggtaact ctgaggtttg 353220 gagatcttga atagcctcat gttcctgaac ttctttacca tacatgaatt acttcttagg 353280 aagaaacatt tattctgtag aatttggttt cgcattttat ttttattttc ttagacactg 353340 gtagttggaa attcaggaag aatttgacta caaaaaacat ttattaaaaa gagtttctaa 353400 tctcacctgc taagtgctac aaggaaaaaa aaaaatacat tttgagccct caaggggctc 353460 caaatacggt tgaaggaaaa ttagacccag aatagtatat gattaggaac aaaacgatgg 353520 cagagaaatg agagtttaga gaaaggagag ataatcctga gttaccttag gaagagaaaa 353580 tttcacagag aaaacggccc ccacactgat ttggggaaga tgattcagac ttgtctcaat 353640 ggcatgagga gcaggggcca gatgggaaag gacatcgtgg tggaaatgac acgatgagca 353700 gaagcttaga agtacttctg cttcacgtct gtgattctgc ctctcccctt gtttctgcag 353760 tgctaagttg agtgtagttc cccacacaaa ccctgagctt tcttcccctc caggctgctc 353820 catcagtcag acaggagtgc cttctcaggg gatcctcttc ctcatcttat cgggccactc 353880 atctcgtcgc agacattccc atactcctgg ttgacttgct cttccctctt tttaactgac 353940 atgagcaggc atcacctctt ccaggaaact tccctgacac caggctgagt ctctctggtg 354000 tcggggaagg cttctgtatt actgcatctg ccattttgat ttaaggtttt ctgctcattc 354060 caactcaaca gtgagctctt tgagggcaga aattgtgtct tattcatttt tcttgttatg 354120 ccctaaaaat gtattgtgat aatatataag taacgtacaa tttaccattt tcacctcttg 354180 aagtgtatag ctcagtggcg ttaagtgcat tcacactgtt gtgcaaccat tatcaccatt 354240 catctccaga actttctcat catcccaggc aaactctgta tccattacac aatagctctc 354300 catttcctcc tgccctcagc ctcaggaaac ctcccatcta ctttctcttt ctacgcgttt 354360 aactactcat atagatggaa tcatccaaca tttttccttt tgtgcctggc ttatttcact 354420 tgacataatg ttttcaaggt tcttttatgt tttcttatgc gtttttatat ctctctatct 354480 ttgatagcac ccaatacata gaagacaatg aatgtttttg tgagtgaaaa cataagacca 354540 gaaagaaata ggatgtcata ttctagggac actcaagaca ctttcttgag gacagaggca 354600 ctgtagcttg gaatttggaa gatgaggttg gcaaggtggg tggggaaagg atggaaaagt 354660 gatacgtttc ttgaagcatg tggatttgct tccataagca gtggtggacc agtgggcacc 354720 cttatctatt aaaagaatga ttttttttgt gactcgtgta gagcactgtc ttagttacct 354780 accccagtgg tagataagag aagcggatta aaaagagatc tgaacctgag gaaatggaga 354840 ctaccaagtt ttttgtaaat atgtctgtta taacatatat tatctagtac ttgcgatgcc 354900 tgtgccaaag catgcttttt ggtttagtta agcctactta gctcgctaat ttcagtaatt 354960 ttggcttgaa ttgcaaaaag ttgatggggg agatggggga gtctctgaca tccttccccc 355020 actcacaggt ttgaaaaata aaattatgaa gagcaaaagg atctttttct gtggtaaatt 355080 gtacttcatg acaataaacg agttgtggtt ttagtggtta atttataaat taggccatct 355140 ggttcttctg taatctaaag atttttatat atatgtaaaa tacaaagttg tcctgggcaa 355200 ctgccaatct ataaaggaga aaacatcttg aactctgtgg agataataaa accatattca 355260 gctgccaaag tgctgctgac atactgtcca atgagacacc caacgccttt gcattacagc 355320 cagcaaggtg tggagctaga tgtattgatc tatttaataa tattagcttc tcttctctgg 355380 gtcggacttg gcccagagca agtgaacagg acctatctat ctccttctca aaagtcacag 355440 ctttcttacc aaattgtaca actcaagcca caaagaaaac aagtcaagga aaggaccaga 355500 ggagcacgag ggcacatgcc ttgttcccag accctgggac cagctccagc tctggctcct 355560 tgcagatccc gctcttctca agttctctgg ctttgatcct agttttctgc cctactattc 355620 ctgacatgta cagcacttag caattcataa cttgctttat taaccaatat ctcaacagaa 355680 cttttaaaca acctgggagg aagcagagtg ggtgttactg tcctcattgc acagataatg 355740 aaactgaggt tcagaaaggt tgagtgtctt gcccaaggcc acacggcttc ttagtggaga 355800 agccaggatc tgcaccaatg tcttccattg gacacactgc cacttaatat ggtacttagt 355860 ttttctttct agatgaagca gtatgaggaa ttctaccatg gatatctctc tctatttttt 355920 taaactgcaa aataatgata atgataataa tgaatacttc tagaatacag tctctatgcc 355980 aggcactata tgctttatat gtactaactt gcttaattct cctaacaacc ctagaaggta 356040 gttactaata ttatcactat ttttaaatgg ggaaactgag ttacagagat ggtaaataag 356100 ttgcccaagg tcacagaggc agccagatta gtgacaaaag tttgagagta ctcagtttga 356160 attttacatg ttccgtggat atggtttatg ccaaatacct tctcctttct agacctcagt 356220 ttcctcatgt atcaaacaag tggactgcac tatacaactc tgaggtccct tctagcctga 356280 agattagagt cttttatgcc cacatgatat ggaaaacatt aatgctggct ctttaagact 356340 ggaaccttgt catttaaaaa aataagctct actaaatttg cagtaaactg tccaaatacg 356400 atctctggtc tctgatttgt atctctcaca gagcatgata caatgctaga cacatatata 356460 ctcattaaaa acttgctgaa ttaaattgta aaacattcct ccagatagac cgtaagggtg 356520 agctgatgcc ctaatatgct cctcagctgc tgacttagga cccgtgggtg acaaaccctg 356580 tccttgttgt tccatctgtg acagtgggca gtgtccaaaa tgagtctctt catcaaaagt 356640 gagtccagga aactggatca gagtaagtaa ggagtcttcc ttgcttcttt ttctctaaaa 356700 tacaatacag tgcattctag caaactacca gcatgcctat aaatctcatt ttaaaaatat 356760 caggaaatag atgctcattg ataaagaggg taagaatgag caagggagag agtcaagcct 356820 gtacatttgt gcttagggta ttttcatttg agaaattatc attggaaaga tcaatttcca 356880 aggcaggcgt tttgtcttga cttaataaac taatctttat caataataat taaataatgt 356940 cattttccca gatacaaagg ggaaaccata atgcctttca tatgccttct catatcttca 357000 gtgtttctca aacccagctg tggggtcttt ttagggaaga tacaaggagg gtgagaccat 357060 gtggagcaca cactcagcag ccactgtgaa gtcatgggtc ccttgctacc tttggaagct 357120 gacaatctgc atgaaaagca gcccctagga acatgggtag tggcccggga tccaaaactc 357180 ctgaatccat aaaagggctt ttgacttctt gttccaggac aagtgacatc tgacatgctt 357240 gcctgcttgt ttgcctttct tccttctcct atccgtcttt ccatccatct gtccatcagt 357300 ctatctgtcc atccagtcat ccatccatcc ttccatccat tcgtctattc atccatccgt 357360 ccttccatcc atccatccaa gatttattga gcacttacat gacaggaagt atgagacaca 357420 aaagaaaatt ctacacaatt ctcctaggga catgaactaa tggttagcaa aaatatatgg 357480 gaagtcctgc ttcaagtaaa attatgactt aagatataca tcagatattt gaaaaagata 357540 tttttaagtt caacattgaa attctaacta cattgtgtcc ttaatttcta ttttctagtt 357600 agactttttt gaagatacaa atcatttgaa ttatcattat tattgttact tgattttatt 357660 tattccacat gaatggtaag aattttaaag gaaggtgggt gccctaaaaa tacatttatg 357720 ccaactggat tgaatcacct tgaatcacaa tgagtacaac aactgctaat gagctaacac 357780 ttaatgagta atgaccaggc accagacact gttctagcac tttatactta ctgatttctt 357840 tactcttcat gataacccta tgaggaaagt gcttttttgt taagctccat tttcagctga 357900 agaagctgag gcctggagaa gttacatagc tttctcatgg tgtctccact atgaagtagc 357960 tgagctgaga tctcacccaa ggccctgctg ggttcaggcc acactcttaa gtactcacag 358020 ctgcatcaca ctgaatatca tctccctgca ctgccatttc atttgagcag ggctttgcct 358080 gtcaagaaca ttcatatctt ttgataaaca gtgcattttt tccctgatag tctgtatggg 358140 acagcctcct tgtttgcatc tgaaggacag ggacttcagg acctcctgct gtggtcacca 358200 aggaatcaca ttcaaacttc tcagcaaggt gttatggtcc ttctcagttt gcctacgatc 358260 cattgaggca aaggtttcct tctctcaata caccctctgc tttagccagg gggacctccc 358320 actgtcctct ctatgtgttc tatttgttcc tgactgttag tacccgcttg tgtggtttcc 358380 atgatccttc ttctgcctgg aatgaggctc ctcttgagtc ctgagagctt catttctgtc 358440 tgtcttttgg gctgtgccaa agcccctctg ccttcgtgga ctccccactg attcattacc 358500 aattttcctc tcctctgggc tcttcttaga ttgcaaatgg tgcctctgga ttgggtacca 358560 agacttgcag tgccttattt tgataccact aatatgagac catgttatat gcctcttgag 358620 atatcattcc ctttctagca atagacagtg ataagcatgc gtatagaggg taaacgtagg 358680 actttgaggt tcatccatct caatagacaa aagtaaaagt atgctaattt caatccttca 358740 cacagtaaat tgaatgtaat aggtcttcag taactatttg aggaatgaaa ttgttattga 358800 tatattgatt taactgagca aatccataag tgccacgctt ggagtaaagt acagggaatc 358860 tcattttttg ggtaatttca aacaagtagg agaccatcca ctatctatta aggacaaagg 358920 agatttgagt gacaaacatg ttccagacca tccaatggtg ttattctact acttcatccc 358980 agctagtaaa atggggcaat aagaaccaaa aagcaggtac tgtccctatg taagtagact 359040 tcctagtcct ggccaaaaca aaaacaaaaa aaaaacaaag caacacaaaa caaaaacaat 359100 gtgtgaagag catcttaccg ttcattgctt tggatgattt gtgcaaatct agttaatggg 359160 ggtggatctg atcagtcttt gtaggaagtc agtattggca tatgtctggc ttctgaattt 359220 tctaaaaata taattattta tttttaaatc cattcaatca acactgaatt tactaatcaa 359280 ctactaaata agtaaaagat cctatagtag atccttcaag aaatggaaat tgagacatta 359340 ggtcactata aaccccaacc cttgtaatct ttttgtggca accttaggaa acttggaatg 359400 ctttttacac tctgataaag gtttaattgt aatcatagcc ccagtgttgc aaaatgagtc 359460 agaagataca aagtatctca taggataata ttatagctgt aggtacttca aatggacaag 359520 tataacacat tatgactgct aatatggatg cattgggagg aaaataagcc ctaagaagca 359580 atatgcagaa aagctatgat tgctggagac agatagatcc aagaacctag gtaatttttc 359640 agcaaactgt ggttccactt tcgcctcttt cttgacattg actgtgtttt taacagggaa 359700 aaagttatct gaaatttttg atagagttca aactttaaat cgtgttcact ttgctaaaac 359760 tccactggat ggagtggcag aagagaaaaa acacagaaat aaaagaaaag cagtaatgga 359820 gaatggaaaa tctttaaaga tgctaccatc ctgaaaagat tcgaaagcaa gcccaggaaa 359880 gccacgggga gggaaagagc gcttagaggt gtccccaaag ctttcagaag tgatgaggga 359940 aaccccagct cttcttgtta cttcttggca ttttgttcct ctatacgtta gcttttggtt 360000 aatctttcat gttacttatt atctctgttt taaaatttca cattaactca ttggtttcat 360060 atcccactta acaaattaga tattttcctt gggcttatta agaaaattat ttgctctcat 360120 atgttatggc tttagatttt ctgtgtagct aggactgttc ttaaattttt atctttacct 360180 ttgttatttc tctgaagcat ccccattggg gtggcagtgg ggggtattaa atggttatgt 360240 aaaatttaca tgtaattgtg caatttacac atagcctcaa atataatgag gcctagtcta 360300 gctgcccata ccaagaggat attatagttg ccaataaaat cagtattatt tctttttttt 360360 ttctgttctt ttttagcttc ttgcaaacat tcactcagaa tcctttttga atatcagttt 360420 tgtgaaaagc accgtgctgg gttctttggg aaaagccaag atgagggcaa atgcatcctc 360480 cttcctagtg agtctagcac acagatgact caggaagtca tgcactggag gaggagtatc 360540 ttctagaact ggggaaaggt taaggaaggg atcagaaacc tttttcactc aaccaattac 360600 attttcctga aagcatgtta ttaatcgtac atgatcttgc agaccccaga tagagactat 360660 atcactatat caattgtttt cttgaacaac tggagttaat tctcatatgt ttggttaccc 360720 ttcaagtatt tatgtttagg atctgttttc ttcctcttaa cttacattcc aaagtttagg 360780 tatccttccc ctccatttgc tgtccaaaac agcatccttt catgtagcta catgccattg 360840 gaatgatttg gtttttgcca tggattccta gtcctaaatt tggactttta ttggtatcta 360900 agatgtcatg gacagaaccc acagatgttg tacaaaaagg gctgagtgga cagtccaaca 360960 gagttacatc atctgttcca aacacccttg taatggttgt atcagcgtca atgtctgagt 361020 tgctgtcctg gttctcctgg ccagtcctca gctggccatg cctgttcagg caccagagaa 361080 agcttcatac ctcaggcaaa tctttacaag ggatttgaaa ggcaagaaac atgtattttg 361140 gaggttttac ctgtttattt catgtatagc ttcatatt~aa cagattttgc tatcacttaa 361200 aagtaaataa aatgattttg atttctcaaa atcatcttat aatttattaa tctattttaa 361260 ggcttgcatt tacatttaat cttcaaatct tgatgcattt tcacagagtt tctttagtta 361320 gttaaggata ttaatgactt cctttaatag ttgatgaagt gagtcatcat cgacagtgag 361380 tcactaaggc caactcttgt acctgctctt aaatagcagt tgttttacta gatttgtact 361440 gaaacctttt taaaaatctg tttgtcttat atataacaag catttccagt agttaataat 361500 actttcttag ctcttcaatc attttatgag agcattacaa gaaaagcagg agatggggaa 361560 agagatatct tgtttgccat gtaggatgca tgaaattctt acttagtctg ttgtgttttg 361620 gattcgagag aaaatatgta atgaatcaaa atgtttccta cagtcctctg aaaagatgta 361680 atgagacagt cttccagcag cctttgatca tctgggaagt cattgacttt gatccttatc 361740 ctatcagggg ctcttaccct ggggtccata aatacccagg catttttaag aagtggattt 361800 cagtataact tgtttcattt tgtgattttt aaaaatattt tgttttatgc acttaaaacc 361860 ttaatctgag aagtcatctg tagactatac cagatttcca aatggaataa aaatatttaa 361920 gaactctctt atgtgtgtgt ttcaacctat aaattttata tgtgtagctc atggttaggt 361980 ggtaagtaga gaatttgcta tctgtgtaat atttggtact ttttataatg aaaaaggaag 362040 taatacaatg ataggtacca tttatttgtt gaccacccat attttccagg gaatgtgata 362100 gatacttttt atatattctt tctagttttc accataacat gctgcaggga tagtgttacc 362160 attctcattt tgcagatgaa gaaattgaaa cccagaacaa ttcgttcatt cattcaaaaa 362220 atatccatag agcatacact aagagaaggc actctgctag gcaccagaaa ttgaataatt 362280 gaccccaaat cacctaactc ataagtggca aagctggacc taacaaagcc cattatccct 362340 tctatcacac attccatcct cataatatca tattctacat agaaaaactt aacagtcctg 362400 ttgagacact tggaactcta agaaggtagt caacagatac ttttctcttt atataaatat 362460 gatatctaat tagcttttcc tggaaaggag tgattctgcc tttttttaat tctagcattc 362520 cctgagcaaa ttagagccca ggtaatgatt tcctgagata agtcaattct cttctaggac 362580 aatctctagt cttgtcatca tgagatggaa taacaacggt ctatgtcatt tcagtgacgg 362640 cacctgcatg tccttgtatc taagaaaaga cacctccttc tctgccttgc taaatatttg 362700 taaactttgc tttaattggt atatgtgcca ctgttttgta tgtgggaggg gtttatggtt 362760 ttgttttgtt ttttggtctt ttttagtaac cccaatccaa actattctca tttgtaacct 362820 aaaatactta gaacaagagg ctttgcaatc ttgaagaccg aatacatttt tactactaaa 362880 tgtaaggcag atttgagcca cccattttgt agtgcttgat tatggcaacc cgaggaaact 362940 aatacggtta ctctttatct ttattaggaa atactgctgt agttcctgtt gtaatcctta 363000 gggagccaac attgttgatg agacctagta ccaagccaga ggccatgttg aactagcaag 363060 atatgggata gtttttactt tcaaagtaat tagcatctag agtctagaga gtttctctta 363120 cagggtggcc tggtgtctta gtctattttc tgttcctata atagaatacc tgagactggg 363180 taatgtacaa attataaaga tttgtttgac tcacaatcct agaggctggg aggttcaaga 363240 tcagacagcc acatctggcc atcctttaat gagggcctca tgctgtgtca taacacagca 363300 gagaattgga aggagaaatg ggcacatgca aagagaaaga aaggggacga aaggggctaa 363360 ctcactttgt aactatgaga ctaatccatt ccctggagaa ctaatttatt ctcatgagaa 363420 agacatgaat acatcttaac atcctaatca cttcttaaag gcctcaactc tgtacactgc 363480 tacattgtaa caggcaatta aatttcagca tgagttttgg tggggacaaa ctacatccca 363540 actgtagtac ctagctaccc actttatttt tagagtggct atttaatgac ctggcaagat 363600 ctactgtgct tgtaaaagaa tccaccatgg gttaaagaaa aatagtttct ggcttccagt 363660 ctccttttgg cctctctcta gaatggcata tacaagttca gtggttactg acttcaaagg 363720 tggggtggaa gacactgaaa tgaatttttc tcctttcttt ggttggttgc tctctctctt 363780 tgttgctgaa atttgtgtag gataatggaa tgaatatggg agatagggaa ctatagaaag 363840 actggaggga agggagaagt cttttctgaa ttaacccccc atgtgcttgt gagacccttg 363900 gaccccaaag ggctcctgac tggctgggcc cactccttct gtatatggag ccatagggct 363960 tttctctggc ttcccagttg aagcattgtc atatgctttg atgctcagca tttctggcaa 364020 ttttaccttg taaaactccc gtttcgacat tctgaactta agtagaaaat gggaatataa 364080 gcctcttgtt ttttgctctt aatccattcc agaactataa gtccctgggt tcttggcaac 364140 aacaggtgtt cctctagatt ttaaatcaag tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg 364200 tgtgtggaat atctcactaa aacgacagtg ttgtcatcaa ttttggcttc atggagggtc 364260 ttgagctggg tggagatggg aggaagctat ccttgtccat gcggtttgga gacttgctct 364320 aaaaaagaga ccgtctactt ccctcgtagt ttttgttttg aagacatttg tagtgtttac 364380 caggagcata cagttatctg gaaaatgcag actgatgaca atagtatttg aaatgctcgt 364440 agatctgctt ctaggttgat ggaaagaatg tatgttccaa ggtacaagtg cttgttgact 364500 tttcggatgc agccagtctg tgctctttgt caggtaactc ggaatcactc cacagtagct 364560 gtccttcctg tccatttgac aagcacttaa aagggcagtc ttaagtactt tacttcatag 364620 tttattaatt ttttcccttc tttagtttgc aatctactct cagaaactga aaaataattt 364680 cttttacctt tttccccttt ctcatttaag ctgaaattga tttctttctg agcgtgtaag 364740 gaaaaaaata aaaagaagct gtggttaata caattgcctt tgctgaacaa aaataaaaga 364800 ctagtttacc ttcaagctgt gctgtatcag agtaaggatc tgttctgtca ctcccaggag 364860 attttgataa gagagagtac aaaatttcat aatatctaca tagcataatt gtagcaaata 364920 taagaagtat tataattgtt tatttgggga tgagggtggg gatgggtttt tttgtgtgtg 364980 tttggaaaat tgggaagcta gaagatactc ttaaatccat cagcttcttc agaactcttc 365040 tcaaatgctt attgtcatga caaccatctc ttcataaacc aaggctctga gtgtaataca 365100 gatggggctg gtaacaaggt aggattgcca tctgctttgt tttctctaag ggacattatt 365160 tttttgtgat ccccaagtct catcctcaaa tctgaaagtc ttgacttatt tttctttctg 365220 gagtcagata gagctgtgag attaattctt ttctcctgac aaactacttc ctcacctgtc 365280 atcaaacaac ttacttgtta aatggaggga aaaaagggta attatgtgtg tgtgtgtgtg 365340 tgtgtgtgtg tgtgtgaaaa caaatataca tattttaatg atttatttat ctgtctactt 365400 tgcaaagtct cctatgaggt acctagtaat tcaataatac agtcaagtgt gagatccaga 365460 aacaagtagg agtgaggcaa tcattttccc atgaaacaag taaaattatt actgcttttg 365520 aacattttaa cacctgtgct tcctgatggt ggggctgcat gaagtttgta agtagtgtca 365580 tcattctctt attaggtaac aaataggagc ataattattt aggagaggaa aaccttttct 365640 tgttaccaaa tttggtgaga aatttgccaa agaaatgaat gcttacagga gggatacagc 365700 ttaatatctt catactcccc tttgtcaaag atacaaatga cattttctca tatcaacttc 365760 ctacaaaaag tgaggccata catttttaaa aagaatatac ccagtatttt aaaagtttgt 365820 atatatcgtg atcagaatta agatatatcc agcttttgaa aaaatataca tatttagcca 365880 ttaaaaggta tgtggtaaac taaactgatg aataaaggtt atgggaaata tttcataaaa 365940 tttatattta aagtaaagaa caatttctct ctttgtatta tagttatttt acataagcta 366000 caatctataa ttctaaacct taatttcttg aagggcttta taagcctcat ttttctttct 366060 aaatagagaa gttcatacac tgcttttatg tcctatatgt tgatgtgtaa aaataatcaa 366120 atactagtgg gtgagtagaa tgtaactaca atatggtaca cacacagaca cacaggatga 366180 ctaaactgtg cacatgagct gatgtccaga gcagggaaaa gctgctaaag aatgtttgaa 366240 gtagagtcag aatgcagttt tgtggttaag accaataatt tttcaaagga gcattataag 366300 gagtgtggtt acttgggttg gagttgaaaa ccaagcaaca gttgggcaga cttgaaacca 366360 gttttgtctt taggtttctt ttcttaatga ttggtctaca atgagttagc cactcatcgt 366420 atttaaggtc ttggtaaagg agagactttc tttgccatgt agtttatacc tcatgtatgc 366480 attacagtag agagctccag gaaatactgc aggccttcta ctggttttta tcctctattt 366540 agataaaaat gaggaagaag tagaattatc ataaacaatc ctgttggctt ttagttgaga 366600 tatactacag aaacatcacc tttgaatctg tatatgtaaa aaaaaaaaat ctcagagtaa 366660 gatataagtt ctgttgcatt gttctgaagg aatgggtaat gtcagaaatg tcttcattac 366720 attacataaa gtaatacaca gtgctgttat cagattgcat agtcaaagtt ggcaagtttg 366780 catatttaca tagatatcat gaccttcatc attcatactt ctttcaagaa atggtcatct 366840 taatatgggg tatttatttg aggcatacac catttatttt aagaagcagt acctttgttt 366900 attgtaaaac ttctggacta aattcttcaa ttttttctca aatgaattca gttttttgtt 366960 ttttttctta ccactggttt ttactgcata gcgtttgcct gaagaacacc actttgtttc 367020 ccaaggcaag tagtcactac aaggcgagtt ttgttctgtc tatcccaagg caaatagaca 367080 gcagcaaaca tagtgtggag ggctgctggg ttcagtagaa aaccatcaac tatttctaat 367140 tgggggtgta tgaagacagc tgcattctgg acatgctaaa catcttgaaa tgcttggagt 367200 gtaataaaac cccaaacgtg ctttcttttc tcatttcttt gcacatgtat tttaggcaaa 367260 atataatcca catgctgttg ttccctctcc ttttaaatca tgaaaaatgt tgtttttata 367320 gggctatttg aggtccagga tgccttttta tcctttttat taggttattt ttatatataa 367380 atgaaaagaa aaacataaac agaaaataca acatgacatc atgaatgagg gcaagcacaa 367440 actgcccaat ttaagtttga agttataaac ctaaaatatt ttaaaacaaa acatttctct 367500 tggtgttttg caaaagtaat gctttcttct attgggcaga aattgcagcc tgttgggatg 367560 aaggagagaa tagaactaat atttattgag tagctaccat ggctttctgg gacatctgct 367620 aa~ccccttaa tttttacagt aaccctaagt ggtaggccag tccatctgca ttttacagac 367680 aggaaatgct ttcagagata ttaattagat ggctgtagac cacagaagaa ttgaaagtca 367740 DEMANDE OU BREVET VOLUMINEUX
LA PRESENTE PARTIE DE CETTE DEMANDE OU CE BREVET COMPREND
PLUS D'UN TOME.

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Claims (17)

Claims That which is claimed is:

1. An isolated peptide consisting of an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence of a variant estrogen receptor protein provided in Figure 2;

(b) a fragment of the amino acid sequence of a variant estrogen receptor protein provided in Figure 2, wherein the fragment comprises at least 10 contiguous amino acids.

2. An isolated peptide comprising an amino acid sequence selected from the group consisting of:

(a) the amino acid sequence of a variant estrogen receptor protein provided in Figure 2;

(b) a fragment of the amino acid sequence of a variant estrogen receptor protein provided in Figure 2, wherein the fragment comprises at least 10 contiguous amino acids.

3. An isolated antibody that selectively binds to a peptide of claim 1.

4. An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:

(a) a nucleotide sequence that encodes the amino acid sequence of a variant estrogen receptor protein provided in Figure 2;

(b) a nucleotide sequence that encodes a fragment of the amino acid sequence of a variant estrogen receptor protein provided in Figure 2; and (c) a nucleic acid molecule that is the complement of a nucleic acid molecule of (a)-(b).

5. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:

(a) a nucleotide sequence that encodes the amino acid sequence of a variant estrogen receptor protein provided in Figure 2;

(b) a nucleotide sequence that encodes a fragment of the amino acid sequence of a variant estrogen receptor protein provided in Figure 2; and (c) a nucleic acid molecule that is the complement of a nucleic acid molecule of (a)-(b).

6. A nucleic acid vector comprising the nucleic acid sequences of claim 4.

7. A nucleic acid vector comprising the nucleic acid sequences of claim 5.

8. A host cell containing the vector of claim 6.

9. A host cell containing the vector of claim 7.

10. A method for producing any of the peptides of claim 1 comprising introducing a nucleotide sequence encoding any of the peptide sequences in (a)-(b) into a host cell, and culturing the host cell under conditions in which the proteins are expressed from the nucleic acid.

11. A method for producing any of the peptides of claim 2 comprising introducing a nucleotide sequence encoding any of the peptide sequences in (a)-(b) into a host cell, and culturing the host cell under conditions in which the proteins axe expressed from the nucleic acid.

12. A method for detecting the presence of any of the peptides of claim 1 in a sample, said method comprising contacting said sample with an agent that specifically allows detection of the presence of the peptide in the sample and then detecting the presence of the peptide.

13. A kit comprising reagents used for the method of claim 12, wherein the reagents comprise an agent that specifically binds to said peptide.

14. A method for detecting the presence of a nucleic acid sequence of claim 4 in a sample, the method comprising contacting the sample with an oligonucleotide that hybridizes to the nucleic acid sequences under stringent conditions and determining whether the oligonucleotide binds to the nucleic acid sequence in the sample.

15. A kit comprising reagents used for the method of claim 14, wherein the reagents comprise a compound that hybridizes under stringent conditions to any of the nucleic acid molecules.

16. A method for identifying an agent that binds to any of the peptides of claim 1, said method comprising contacting the peptide with an agent and assaying the contacted mixture to determine whether a complex is formed with the agent bound to the peptide.

17. A method of identifying an individual having or at risk of developing a disorder mediated by a variant estrogen receptor comprising the step of analyzing nucleic acid molecules isolated from said individual for alterations in the estrogen receptor gene sequence, wherein an alteration in said estrogen receptor gene selected from the group consisting of the variants provided in Figure 2 and Figure 4 identifies an individual as having or at risk of developing said bone disorder.