CA2407563A1 - Procede permettant de determiner les concentrations d'amylase - Google Patents
Procede permettant de determiner les concentrations d'amylase Download PDFInfo
- Publication number
- CA2407563A1 CA2407563A1 CA002407563A CA2407563A CA2407563A1 CA 2407563 A1 CA2407563 A1 CA 2407563A1 CA 002407563 A CA002407563 A CA 002407563A CA 2407563 A CA2407563 A CA 2407563A CA 2407563 A1 CA2407563 A1 CA 2407563A1
- Authority
- CA
- Canada
- Prior art keywords
- specimen
- amylase
- sample
- starch
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Ce procédé permettant de déterminer les concentrations d'amylase comprend les étapes suivantes : (a) on incube une première partie d'un spécimen dont la concentration en amylase est inconnue à une température spécifiée, dans une solution d'amidon tamponnée contenant de l'amidon à une concentration comprise entre 20 et 200 mg/litre; (b) on laisse réagir ce mélange pendant un intervalle de temps spécifié; (c) on ajoute une portion de solution aqueuse d'iode à ce mélange de manière à obtenir un premier échantillon; (d) on prépare un second échantillon en ajoutant une seconde partie du spécimen à un mélange comprenant une seconde portion de solution d'iode et une seconde portion de solution d'amidon, (e) on mesure la densité optique des deux échantillons à une longueur d'ondes comprise entre 300 et 800 nm; et (f) on calcule l'activité de l'amylase dans le spécimen à l'aide d'une courbe d'étalonnage. Le premier et le second échantillon contiennent des concentrations identiques en spécimen et en solution d'amidon. Le temps nécessaire à la mise en oeuvre de ce procédé est inférieur à 10 minutes, et de préférence inférieur à 6 minutes. Ce procédé convient pour surveiller la concentration d'amylase dans les procédés de nettoyage industriel.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00201500.6 | 2000-04-26 | ||
| EP00201500 | 2000-04-26 | ||
| PCT/EP2001/004360 WO2001081617A1 (fr) | 2000-04-26 | 2001-04-17 | Procede permettant de determiner les concentrations d'amylase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2407563A1 true CA2407563A1 (fr) | 2001-11-01 |
Family
ID=8171410
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002407563A Abandoned CA2407563A1 (fr) | 2000-04-26 | 2001-04-17 | Procede permettant de determiner les concentrations d'amylase |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1278888A1 (fr) |
| JP (1) | JP2003530891A (fr) |
| AU (1) | AU2001260219A1 (fr) |
| BR (1) | BR0110289A (fr) |
| CA (1) | CA2407563A1 (fr) |
| TR (1) | TR200202424T2 (fr) |
| WO (1) | WO2001081617A1 (fr) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20140013078A (ko) * | 2004-11-07 | 2014-02-04 | 쿠프론 인코포레이티드 | 상처, 화상 및 다른 피부 질환을 치료하기 위한 구리 함유 물질 |
| US20100240082A1 (en) | 2009-03-18 | 2010-09-23 | Syngenta Participations Ag | Methods for detecting and measuring polysaccharide-hydrolyzing enzymes |
| FI20165270A (fi) * | 2016-03-31 | 2017-10-01 | Kemira Oyj | Menetelmä ja järjestelmä tärkin määrittämiseksi näytteestä |
| WO2017208249A1 (fr) * | 2016-05-31 | 2017-12-07 | Indian Institute Of Technology, Guwahati | Système/kit à base de transmittance pour la quantification au point d'intervention d'échantillons de biomarqueurs et son utilisation |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3888739A (en) * | 1973-05-07 | 1975-06-10 | Bio Reagents & Diagnostics Inc | Reagents and methods for determining amylase concentrations |
| JPS6058099A (ja) * | 1983-09-12 | 1985-04-04 | Konishiroku Photo Ind Co Ltd | α−アミラ−ゼ乾式分析素子 |
-
2001
- 2001-04-17 AU AU2001260219A patent/AU2001260219A1/en not_active Abandoned
- 2001-04-17 CA CA002407563A patent/CA2407563A1/fr not_active Abandoned
- 2001-04-17 EP EP01933841A patent/EP1278888A1/fr not_active Withdrawn
- 2001-04-17 TR TR2002/02424T patent/TR200202424T2/xx unknown
- 2001-04-17 JP JP2001578688A patent/JP2003530891A/ja active Pending
- 2001-04-17 WO PCT/EP2001/004360 patent/WO2001081617A1/fr not_active Application Discontinuation
- 2001-04-17 BR BR0110289-3A patent/BR0110289A/pt not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2001260219A1 (en) | 2001-11-07 |
| EP1278888A1 (fr) | 2003-01-29 |
| WO2001081617A1 (fr) | 2001-11-01 |
| BR0110289A (pt) | 2003-02-18 |
| JP2003530891A (ja) | 2003-10-21 |
| TR200202424T2 (tr) | 2003-02-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |