CA2406466A1 - Reconstruction d'acides nucleiques n'utilisant pas la pcr - Google Patents

Reconstruction d'acides nucleiques n'utilisant pas la pcr Download PDF

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Publication number
CA2406466A1
CA2406466A1 CA002406466A CA2406466A CA2406466A1 CA 2406466 A1 CA2406466 A1 CA 2406466A1 CA 002406466 A CA002406466 A CA 002406466A CA 2406466 A CA2406466 A CA 2406466A CA 2406466 A1 CA2406466 A1 CA 2406466A1
Authority
CA
Canada
Prior art keywords
stranded
double
dna
nucleic acid
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002406466A
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English (en)
Inventor
Alexander Volkov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2406466A1 publication Critical patent/CA2406466A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés pour former des acides nucléiques de reconstruction qui ne nécessitent pas de réaction en chaîne de la polymérase (PCR) pour cet assemblage ou cette reconstruction. Ainsi, les acides nucléiques de reconstruction ne sont pas soumis à des limitations de taille ni à une amplification des erreurs indésirables. De plus, la présente invention permet de recombiner des séquences avec une faible homologie, ce qui ne serait pas possible avec les fortes températures de la PCR. En outre, l'invention concerne des procédés permettant de former des protéines, des dosages, des réserves de ressources génétiques et des protéines et des acides nucléiques de reconstruction.
CA002406466A 2000-04-21 2001-03-28 Reconstruction d'acides nucleiques n'utilisant pas la pcr Abandoned CA2406466A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55720800A 2000-04-21 2000-04-21
US09/557,208 2000-04-21
PCT/US2001/009971 WO2001081568A1 (fr) 2000-04-21 2001-03-28 Reconstruction d'acides nucleiques n'utilisant pas la pcr

Publications (1)

Publication Number Publication Date
CA2406466A1 true CA2406466A1 (fr) 2001-11-01

Family

ID=24224471

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002406466A Abandoned CA2406466A1 (fr) 2000-04-21 2001-03-28 Reconstruction d'acides nucleiques n'utilisant pas la pcr

Country Status (4)

Country Link
EP (1) EP1276858A1 (fr)
AU (1) AU2001253001A1 (fr)
CA (1) CA2406466A1 (fr)
WO (1) WO2001081568A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951719B1 (en) 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
AU2011338841B2 (en) 2010-11-12 2017-02-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (fr) 2010-11-12 2012-05-18 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
EP2748318B1 (fr) * 2011-08-26 2015-11-04 Gen9, Inc. Compositions et procédés pour un assemblage haute-fidélité d'acides nucléiques
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
LT2841601T (lt) 2012-04-24 2019-07-10 Gen9, Inc. Nukleorūgščių rūšiavimo būdai ir multipleksinis preparatyvinis in vitro klonavimas
WO2014004393A1 (fr) 2012-06-25 2014-01-03 Gen9, Inc. Procédés d'assemblage d'acides nucléiques et de séquençage à haut débit
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3614310A1 (de) * 1986-04-28 1987-10-29 Hoechst Ag Verfahren zur isolierung mutierter gene sowie der entsprechenden wildtyp-gene
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
JPH1066576A (ja) * 1996-08-07 1998-03-10 Novo Nordisk As 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法
AU1350801A (en) * 1999-10-27 2001-05-08 Frances Arnold Production of functional hybrid genes and proteins

Also Published As

Publication number Publication date
AU2001253001A1 (en) 2001-11-07
WO2001081568A1 (fr) 2001-11-01
EP1276858A1 (fr) 2003-01-22

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued