CA2379326A1 - Variantes a glycosylation reduite de cellobiohydrolase : cbhin45a, cbhin270a et cbhin384a - Google Patents

Variantes a glycosylation reduite de cellobiohydrolase : cbhin45a, cbhin270a et cbhin384a Download PDF

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Publication number
CA2379326A1
CA2379326A1 CA002379326A CA2379326A CA2379326A1 CA 2379326 A1 CA2379326 A1 CA 2379326A1 CA 002379326 A CA002379326 A CA 002379326A CA 2379326 A CA2379326 A CA 2379326A CA 2379326 A1 CA2379326 A1 CA 2379326A1
Authority
CA
Canada
Prior art keywords
gly
thr
ser
asn
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379326A
Other languages
English (en)
Inventor
William S. Adney
Stephen R. Decker
Suzanne Lantz Mccarter
John O. Baker
Rafael Nieves
Michael E. Himmel
Todd B. Vinzant
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Midwest Research Institute
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379326A1 publication Critical patent/CA2379326A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)

Abstract

La présente invention concerne un procédé permettant de produire une exoglucanase active dans un système hôte hétérologue eucaryote. Ce procédé consiste à réduire la glycosylation de l'exoglucanase par remplacement d'un résidu d'acide aminé à site de N-glycosylation par un résidu d'acide aminé non glycosyle accepteur. Cette invention concerne également une cellobiohydrolase, comprenant les enzymes de cellobiose variantes à glycosylation réduite CBHIN45A, CBHIN270A ou CBHIN384A, ou toute combinaison de ces dernières.
CA002379326A 1999-07-13 2000-07-13 Variantes a glycosylation reduite de cellobiohydrolase : cbhin45a, cbhin270a et cbhin384a Abandoned CA2379326A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14371199P 1999-07-13 1999-07-13
US60/143,711 1999-07-13
PCT/US2000/019007 WO2001004284A1 (fr) 1999-07-13 2000-07-13 Variantes a glycosylation reduite de cellobiohydrolase : cbhin45a, cbhin270a et cbhin384a

Publications (1)

Publication Number Publication Date
CA2379326A1 true CA2379326A1 (fr) 2001-01-18

Family

ID=22505256

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379326A Abandoned CA2379326A1 (fr) 1999-07-13 2000-07-13 Variantes a glycosylation reduite de cellobiohydrolase : cbhin45a, cbhin270a et cbhin384a

Country Status (4)

Country Link
EP (1) EP1200574A4 (fr)
AU (1) AU6210800A (fr)
CA (1) CA2379326A1 (fr)
WO (1) WO2001004284A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8637293B2 (en) 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
US7375197B2 (en) * 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
AU2002316809A1 (en) 2001-06-26 2003-01-08 Novozymes A/S Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
EP2305800B1 (fr) * 2002-08-16 2015-05-27 Danisco US Inc. Nouvelles cellulases CBH1 à variante Hyprocrea Jecorina
EP2311942A1 (fr) * 2003-03-21 2011-04-20 Genecor International, Inc. Nouvelles cellulases CBH1 à variante Hyprocrea Jecorina
PL1627050T3 (pl) * 2003-04-01 2013-10-31 Danisco Us Inc Wariant CBH1.1 Humicola grisea
ATE458041T1 (de) * 2003-05-29 2010-03-15 Genencor Int Neue trichoderma-gene
DK2377931T3 (da) 2003-08-25 2013-07-08 Novozymes Inc Varianter af glycosidhydrolase
EP2357227B1 (fr) 2010-02-11 2015-07-15 Süd-Chemie IP GmbH & Co. KG Enzymes de cellulase optimisées
BR112015013647A2 (pt) 2012-12-12 2017-11-14 Danisco Us Inc variante isolada de uma enzima celobio-hidrolase (cbh) parental, polinucleotídeo isolado, vetor, célula hospedeira, composição detergente, aditivo alimentar, método para hidrolisar um substrato celulósico, sobrenadante de cultura celular, métodos de produção de um polipeptídeo cbh variante e sobrenadante de cultura celular

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4472504A (en) * 1983-03-28 1984-09-18 The United States Of America As Represented By The Secretary Of The Army Hyperproducing cellulase microorganism
JPS6027384A (ja) * 1983-07-27 1985-02-12 Res Assoc Petroleum Alternat Dev<Rapad> セルラ−ゼの製造方法
CA1338400C (fr) * 1983-08-31 1996-06-18 David H. Gelfand Cellulases fongiques recombinantes
US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
EP1431389A3 (fr) * 1992-10-06 2004-06-30 Novozymes A/S Variants de cellulases

Also Published As

Publication number Publication date
EP1200574A1 (fr) 2002-05-02
AU6210800A (en) 2001-01-30
WO2001004284A1 (fr) 2001-01-18
EP1200574A4 (fr) 2004-03-17

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued