CA2365099A1 - Transgenic plants that are resistant to a broad spectrum of pathogens - Google Patents

Transgenic plants that are resistant to a broad spectrum of pathogens Download PDF

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Publication number
CA2365099A1
CA2365099A1 CA002365099A CA2365099A CA2365099A1 CA 2365099 A1 CA2365099 A1 CA 2365099A1 CA 002365099 A CA002365099 A CA 002365099A CA 2365099 A CA2365099 A CA 2365099A CA 2365099 A1 CA2365099 A1 CA 2365099A1
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Prior art keywords
peptide
amino acid
transgenic plant
group
nucleic acid
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CA002365099A
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French (fr)
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CA2365099C (en
Inventor
Santosh Misra
William W. Kay
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Victoria University of Innovation and Development Corp
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Publication of CA2365099C publication Critical patent/CA2365099C/en
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Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/463—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from amphibians
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8281—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for bacterial resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Transgenic plants that express dermaseptin and/or temporin peptides are disclosed. In certain embodiments, these plants have enhanced, broad-spectru m pathogen resistance and are useful as agricultural or horticultural crops. I n other embodiments, the plants are used to produce large quantities of the dermaseptin and/or temporin peptides.

Claims (14)

1. A transgenic plant expressing a cationic peptide selected from the group consisting of:
(a) temporins; and (b) dermaseptins.
2. A transgenic plant comprising a recombinant nucleic acid molecule, wherein the nucleic acid molecule encodes a peptide selected from the group consisting of:
(a) temporins; and (b) dermaseptins.
3. A transgenic plant according to claim 2 wherein peptide comprises an amino acid sequence selected from the group consisting of the amino acid sequences set forth in SEQ IDs: 3-14, and 17-26.
4. A transgenic plant according to claim 3 wherein the peptide further comprises an N terminal peptide extension of between 2 and 25 amino acids in length.
5. A transgenic plant according to claim 4 wherein the N terminal peptide extension is selected from the group consisting of AMWK, ASRH, and ALWK.
6. A transgenic plant comprising a recombinant nucleic acid molecule, wherein the nucleic acid molecule encodes a fusion peptide having a formula selected from the group consisting of:
(a) P-D ; and (b) P-T, wherein D is a dermaseptin peptide, T is a temporin peptide and P is an anionic pro-region peptide.
7. A transgenic plant comprising a recombinant nucleic acid molecule, wherein the nucleic acid molecule encodes a fusion peptide having a formula selected from the group consisting of:
(a) P-S-D ; and (b) P-S-T, wherein D is a dermaseptin peptide, T is a temporin peptide, P is an anionic pro-region peptide and S is a spacer peptide.
8. A transgenic plant comprising a nucleic acid molecule encoding a peptide comprising an amino acid sequence selected from the group consisting of (a) SEQ IDs: 3-14 and fragments thereof;
(b) amino acid sequences that differ from an amino acid sequence specified in (a) by one or more conservative amino acid substitutions; and (c) amino acid sequences that share at least 40% sequence identity with an amino acid sequence specified in (a), wherein the peptide has dermaseptin biological activity.
9. A transgenic plant according to claim 8 wherein the peptide further comprises an anionic pro-region peptide operably linked to the N-terminus of the peptide.
10. A transgenic plant comprising a nucleic acid molecule encoding a peptide comprising an amino acid sequence selected from the group consisting o~
(a) SEQ IDs: 17-26 and fragments thereof;
(b) amino acid sequences that differ from an amino acid sequence specified in (a) by one or more conservative amino acid substitutions; and (c) amino acid sequences that share at least 50% sequence identity with an amino acid sequence specified in (a), wherein the peptide has temporin biological activity.
11. A transgenic plant according to claim 8 wherein the peptide further comprises an anionic pro-region peptide operably linked to the N-terminus of the peptide.
12. A transgenic plant comprising a recombinant nucleic acid molecule encoding a peptide comprising an amino acid sequence selected from the group consisting of SEQ IDs: 28 and 34.
13. A method of producing a biologically active cationic peptide comprising:
providing a transgenic plant according to claim 1; and isolating at least one biologically active cationic peptide from the plant.
14. The method of claim 13, wherein the cationic peptide is selected from the group consisting of the dermaseptins set forth in SEQ ID NOs 3-14.
and the temporins set forth in SEQ ID NOs 17-26.
CA2365099A 1999-03-17 2000-03-16 Transgenic plants that are resistant to a broad spectrum of pathogens Expired - Fee Related CA2365099C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12507299P 1999-03-17 1999-03-17
US60/125,072 1999-03-17
PCT/CA2000/000288 WO2000055337A1 (en) 1999-03-17 2000-03-16 Transgenic plants that are resistant to a broad spectrum of pathogens

Publications (2)

Publication Number Publication Date
CA2365099A1 true CA2365099A1 (en) 2000-09-21
CA2365099C CA2365099C (en) 2012-05-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2365099A Expired - Fee Related CA2365099C (en) 1999-03-17 2000-03-16 Transgenic plants that are resistant to a broad spectrum of pathogens

Country Status (6)

Country Link
EP (1) EP1163352A1 (en)
JP (1) JP2002538828A (en)
CN (1) CN1351667A (en)
AU (1) AU772964C (en)
CA (1) CA2365099C (en)
WO (1) WO2000055337A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2853538A1 (en) 2013-09-27 2015-04-01 Université Pierre et Marie Curie (Paris 6) Analogues of temporin-SHa and uses thereof
US10743538B2 (en) * 2014-10-01 2020-08-18 Plant Health Care, Inc. Elicitor peptides having disrupted hypersensitive response box and use thereof
CN106397568B (en) * 2016-12-02 2019-10-01 河南牧翔动物药业有限公司 A kind of cecropin D ermaseptin-M and its application
FR3061178B1 (en) * 2016-12-22 2021-02-12 Univ Pierre Et Marie Curie Paris 6 Upmc ANTIMICROBIAL PEPTIDES AND THEIR USES

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU648140B2 (en) * 1991-02-01 1994-04-14 Virtual Drug Development, Inc. Reverse antimicrobial peptides and antimicrobial compositions
GB9127250D0 (en) * 1991-12-23 1992-02-19 Unilever Plc Modification
US5789377A (en) * 1992-08-21 1998-08-04 University Of British Columbia Treatment of endotoxin-associated disorders with cationic peptides
US5607914A (en) * 1993-01-13 1997-03-04 Pioneer Hi-Bred International, Inc. Synthetic antimicrobial peptides
US6750381B2 (en) * 1996-03-25 2004-06-15 National Institute Of Agrobiological Sciences Pathogen-resistant plants transformed with a DNA encoding sarcotoxin 1A linked to a signal peptide and a method for production thereof
JP2000516812A (en) * 1996-08-14 2000-12-19 ノバルティス アクチエンゲゼルシャフト Peptides with inhibitory activity against phytopathogenic fungi
US6503881B2 (en) * 1996-08-21 2003-01-07 Micrologix Biotech Inc. Compositions and methods for treating infections using cationic peptides alone or in combination with antibiotics
SE9604593D0 (en) * 1996-12-13 1996-12-13 Sbl Vaccin Ab Antimicrobially active polypeptides
FR2762850B1 (en) * 1997-05-02 2000-02-11 Biocem RECOMBINANT LACTOFERRINS, THEIR METHODS OF PRODUCTION BY PLANTS AND THEIR USES
AU739960B2 (en) * 1997-07-31 2001-10-25 Sanford Scientific, Inc. Expression of antimicrobial peptide genes in plants, and their use in creating resistance to multiple plant pathogens
BR9914922A (en) * 1998-10-30 2001-07-10 Interlink Biotechnologies Llc Methods to reduce the extent of protease degradation of a protein applied to or produced by a plant, to inhibit the growth of a plant pathogen and to produce a plant, peptide, nucleic acid molecule, nucleic acid segment, nucleic acid construction , transgenic plant, seed, plant cell, and, composition for use in protecting a peptide, polypeptide or protein from protease degradation.
AU1255300A (en) * 1998-11-20 2000-06-13 Micrologix Biotech, Inc. Efficient methods for producing antimicrobial cationic peptides in host cells

Also Published As

Publication number Publication date
AU772964C (en) 2004-10-07
CN1351667A (en) 2002-05-29
WO2000055337A1 (en) 2000-09-21
AU772964B2 (en) 2004-05-13
EP1163352A1 (en) 2001-12-19
JP2002538828A (en) 2002-11-19
AU3267900A (en) 2000-10-04
CA2365099C (en) 2012-05-29

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