CA2358326A1 - Methode de separation de cellules par centrifugation a gradient de densite discontinue - Google Patents
Methode de separation de cellules par centrifugation a gradient de densite discontinue Download PDFInfo
- Publication number
- CA2358326A1 CA2358326A1 CA002358326A CA2358326A CA2358326A1 CA 2358326 A1 CA2358326 A1 CA 2358326A1 CA 002358326 A CA002358326 A CA 002358326A CA 2358326 A CA2358326 A CA 2358326A CA 2358326 A1 CA2358326 A1 CA 2358326A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- particles
- density
- dense particles
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3616—Batch-type treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/491—Blood by separating the blood components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0427—Platelets; Thrombocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M2202/00—Special media to be introduced, removed or treated
- A61M2202/04—Liquids
- A61M2202/0413—Blood
- A61M2202/0439—White blood cells; Leucocytes
- A61M2202/0443—Macrophages, e.g. monocytes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Vascular Medicine (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Tropical Medicine & Parasitology (AREA)
- Anesthesiology (AREA)
- Cardiology (AREA)
- Public Health (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002358326A CA2358326A1 (fr) | 2001-10-01 | 2001-10-01 | Methode de separation de cellules par centrifugation a gradient de densite discontinue |
CA2405881A CA2405881C (fr) | 2001-10-01 | 2002-10-01 | Methode de separation de cellules |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002358326A CA2358326A1 (fr) | 2001-10-01 | 2001-10-01 | Methode de separation de cellules par centrifugation a gradient de densite discontinue |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2358326A1 true CA2358326A1 (fr) | 2003-04-01 |
Family
ID=4170182
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002358326A Abandoned CA2358326A1 (fr) | 2001-10-01 | 2001-10-01 | Methode de separation de cellules par centrifugation a gradient de densite discontinue |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2358326A1 (fr) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012024691A1 (fr) * | 2010-08-20 | 2012-02-23 | President And Fellows Of Harvard College | Systèmes multiphasiques pour analyse de matériaux solides |
EP2584360A1 (fr) * | 2007-12-05 | 2013-04-24 | Zyomyx Inc. | Kit dýanalyse cellulaire et procédé |
WO2019010186A1 (fr) * | 2017-07-03 | 2019-01-10 | Trustees Of Tufts College | Système aqueux à deux phases pour la séparation et la récupération de cellules de mammifères à partir de cultures contaminées |
EP3440193A4 (fr) * | 2016-04-07 | 2020-03-04 | Mesotex, Inc. | Procédé d'isolement de cellules nucléées et de populations de cellules nucléées et leurs utilisations |
-
2001
- 2001-10-01 CA CA002358326A patent/CA2358326A1/fr not_active Abandoned
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2584360A1 (fr) * | 2007-12-05 | 2013-04-24 | Zyomyx Inc. | Kit dýanalyse cellulaire et procédé |
WO2012024691A1 (fr) * | 2010-08-20 | 2012-02-23 | President And Fellows Of Harvard College | Systèmes multiphasiques pour analyse de matériaux solides |
WO2012024693A1 (fr) * | 2010-08-20 | 2012-02-23 | President And Fellows Of Harvard College | Séparation d'analytes biologiques en fonction de leur densité à l'aide de systèmes multiphasiques |
US9176105B2 (en) | 2010-08-20 | 2015-11-03 | President And Fellows Of Harvard College | Density-based separation of biological analytes using multiphase systems |
US9714934B2 (en) | 2010-08-20 | 2017-07-25 | President And Fellows Of Harvard College | Multiphase systems and uses thereof |
US9857353B2 (en) | 2010-08-20 | 2018-01-02 | President And Fellows Of Harvard College | Kit for density-based separation of biological analytes using multiphase systems |
US10436768B2 (en) | 2010-08-20 | 2019-10-08 | President And Fellows Of Harvard College | Density-based separation of biological analytes using mutliphase systems |
US10732167B2 (en) | 2010-08-20 | 2020-08-04 | President And Fellows Of Harvard College | Multiphase systems and uses thereof |
EP3440193A4 (fr) * | 2016-04-07 | 2020-03-04 | Mesotex, Inc. | Procédé d'isolement de cellules nucléées et de populations de cellules nucléées et leurs utilisations |
WO2019010186A1 (fr) * | 2017-07-03 | 2019-01-10 | Trustees Of Tufts College | Système aqueux à deux phases pour la séparation et la récupération de cellules de mammifères à partir de cultures contaminées |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |