CA2358326A1 - Methode de separation de cellules par centrifugation a gradient de densite discontinue - Google Patents

Methode de separation de cellules par centrifugation a gradient de densite discontinue Download PDF

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Publication number
CA2358326A1
CA2358326A1 CA002358326A CA2358326A CA2358326A1 CA 2358326 A1 CA2358326 A1 CA 2358326A1 CA 002358326 A CA002358326 A CA 002358326A CA 2358326 A CA2358326 A CA 2358326A CA 2358326 A1 CA2358326 A1 CA 2358326A1
Authority
CA
Canada
Prior art keywords
cells
particles
density
dense particles
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002358326A
Other languages
English (en)
Inventor
Steven M. Woodside
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
StemCell Technologies Inc
Original Assignee
StemCell Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by StemCell Technologies Inc filed Critical StemCell Technologies Inc
Priority to CA002358326A priority Critical patent/CA2358326A1/fr
Priority to CA2405881A priority patent/CA2405881C/fr
Publication of CA2358326A1 publication Critical patent/CA2358326A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3616—Batch-type treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A61M1/3693—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits using separation based on different densities of components, e.g. centrifuging
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0427—Platelets; Thrombocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M2202/00—Special media to be introduced, removed or treated
    • A61M2202/04—Liquids
    • A61M2202/0413—Blood
    • A61M2202/0439—White blood cells; Leucocytes
    • A61M2202/0443—Macrophages, e.g. monocytes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Vascular Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Anesthesiology (AREA)
  • Cardiology (AREA)
  • Public Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002358326A 2001-10-01 2001-10-01 Methode de separation de cellules par centrifugation a gradient de densite discontinue Abandoned CA2358326A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002358326A CA2358326A1 (fr) 2001-10-01 2001-10-01 Methode de separation de cellules par centrifugation a gradient de densite discontinue
CA2405881A CA2405881C (fr) 2001-10-01 2002-10-01 Methode de separation de cellules

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002358326A CA2358326A1 (fr) 2001-10-01 2001-10-01 Methode de separation de cellules par centrifugation a gradient de densite discontinue

Publications (1)

Publication Number Publication Date
CA2358326A1 true CA2358326A1 (fr) 2003-04-01

Family

ID=4170182

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002358326A Abandoned CA2358326A1 (fr) 2001-10-01 2001-10-01 Methode de separation de cellules par centrifugation a gradient de densite discontinue

Country Status (1)

Country Link
CA (1) CA2358326A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024693A1 (fr) * 2010-08-20 2012-02-23 President And Fellows Of Harvard College Séparation d'analytes biologiques en fonction de leur densité à l'aide de systèmes multiphasiques
EP2584360A1 (fr) * 2007-12-05 2013-04-24 Zyomyx Inc. Kit dýanalyse cellulaire et procédé
WO2019010186A1 (fr) * 2017-07-03 2019-01-10 Trustees Of Tufts College Système aqueux à deux phases pour la séparation et la récupération de cellules de mammifères à partir de cultures contaminées
EP3440193A4 (fr) * 2016-04-07 2020-03-04 Mesotex, Inc. Procédé d'isolement de cellules nucléées et de populations de cellules nucléées et leurs utilisations

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2584360A1 (fr) * 2007-12-05 2013-04-24 Zyomyx Inc. Kit dýanalyse cellulaire et procédé
WO2012024693A1 (fr) * 2010-08-20 2012-02-23 President And Fellows Of Harvard College Séparation d'analytes biologiques en fonction de leur densité à l'aide de systèmes multiphasiques
WO2012024691A1 (fr) * 2010-08-20 2012-02-23 President And Fellows Of Harvard College Systèmes multiphasiques pour analyse de matériaux solides
US9176105B2 (en) 2010-08-20 2015-11-03 President And Fellows Of Harvard College Density-based separation of biological analytes using multiphase systems
US9714934B2 (en) 2010-08-20 2017-07-25 President And Fellows Of Harvard College Multiphase systems and uses thereof
US9857353B2 (en) 2010-08-20 2018-01-02 President And Fellows Of Harvard College Kit for density-based separation of biological analytes using multiphase systems
US10436768B2 (en) 2010-08-20 2019-10-08 President And Fellows Of Harvard College Density-based separation of biological analytes using mutliphase systems
US10732167B2 (en) 2010-08-20 2020-08-04 President And Fellows Of Harvard College Multiphase systems and uses thereof
EP3440193A4 (fr) * 2016-04-07 2020-03-04 Mesotex, Inc. Procédé d'isolement de cellules nucléées et de populations de cellules nucléées et leurs utilisations
WO2019010186A1 (fr) * 2017-07-03 2019-01-10 Trustees Of Tufts College Système aqueux à deux phases pour la séparation et la récupération de cellules de mammifères à partir de cultures contaminées

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Legal Events

Date Code Title Description
FZDE Discontinued