CA2336062A1 - Bioreactor and cell culturing processes using the bioreactor - Google Patents

Bioreactor and cell culturing processes using the bioreactor Download PDF

Info

Publication number
CA2336062A1
CA2336062A1 CA002336062A CA2336062A CA2336062A1 CA 2336062 A1 CA2336062 A1 CA 2336062A1 CA 002336062 A CA002336062 A CA 002336062A CA 2336062 A CA2336062 A CA 2336062A CA 2336062 A1 CA2336062 A1 CA 2336062A1
Authority
CA
Canada
Prior art keywords
bioreactor
culture vessel
circular edge
bottom circular
axis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002336062A
Other languages
French (fr)
Other versions
CA2336062C (en
Inventor
Yow-Min D. Tsao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KBR Wyle Services LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2336062A1 publication Critical patent/CA2336062A1/en
Application granted granted Critical
Publication of CA2336062C publication Critical patent/CA2336062C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10—Rotating vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A bioreactor and cell culturing method using the bioreactor have been developed to enhance cell production and growth and decrease the shear forces on the cells and multicellular aggregates. The bioreactor contains a dome-shaped culture vessel (1) having walls defining an interior volume (2), an apex (5), a bottom circular edge (3), and an axis of symmetry perpendicular to the bottom circular edge (3);
and a gas-permeable membrane (6) fluidtightly integrated with the bottom circular edge (3) of the culture vessel (1). The bioreactor may further contain a rotating base (7) fluidtightly integrated with the gas-permeable membrane (6), wherein the rotating base (7) is fixed to rotate about the axis of symmetry of the dome-shaped culture vessel (1) set on a substantially horizontal axis.
CA002336062A 1998-06-29 1999-06-28 Bioreactor and cell culturing processes using the bioreactor Expired - Lifetime CA2336062C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/106,987 1998-06-29
US09/106,987 US6001642A (en) 1998-06-29 1998-06-29 Bioreactor and cell culturing processes using the bioreactor
PCT/US1999/014972 WO2000000586A1 (en) 1998-06-29 1999-06-28 Bioreactor and cell culturing processes using the bioreactor

Publications (2)

Publication Number Publication Date
CA2336062A1 true CA2336062A1 (en) 2000-01-06
CA2336062C CA2336062C (en) 2009-04-07

Family

ID=22314273

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002336062A Expired - Lifetime CA2336062C (en) 1998-06-29 1999-06-28 Bioreactor and cell culturing processes using the bioreactor

Country Status (5)

Country Link
US (1) US6001642A (en)
EP (1) EP1093513A4 (en)
AU (1) AU4965799A (en)
CA (1) CA2336062C (en)
WO (1) WO2000000586A1 (en)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050266548A1 (en) * 1995-03-28 2005-12-01 Kbi Biopharma, Inc. Biocatalyst chamber encapsulation system for bioremediation and fermentation with improved rotor
US6720178B1 (en) 2000-06-29 2004-04-13 University Of Louisville Research Foundation, Inc. Self-feeding roller bottle
US6607910B1 (en) * 2000-11-13 2003-08-19 Synthecon, Inc. Two chamber cell culture vessel
US6642019B1 (en) 2000-11-22 2003-11-04 Synthecan, Inc. Vessel, preferably spherical or oblate spherical for growing or culturing cells, cellular aggregates, tissues and organoids and methods for using same
US6589780B2 (en) 2000-12-15 2003-07-08 Council Of Scientific And Industrial Research Bio-reactor for enhancing the biomass yield of plant organs
US6403369B1 (en) 2001-01-19 2002-06-11 Gary W. Wood Cell culture vessel
US6867040B2 (en) * 2001-03-02 2005-03-15 Charles E. Helmstetter Bioreactor and methods for producing synchronous cells
US7771716B2 (en) 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US20050095228A1 (en) 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
JP4653952B2 (en) 2001-12-07 2011-03-16 サイトリ セラピューティクス インコーポレイテッド System and method for treating a patient with treated lipoaspirate cells
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US20050048035A1 (en) 2001-12-07 2005-03-03 Fraser John K. Methods of using regenerative cells in the treatment of stroke and related diseases and disorders
US7651684B2 (en) 2001-12-07 2010-01-26 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
US6986505B2 (en) * 2002-01-24 2006-01-17 Platt Robert E Adjustable fence rail mounting clip
CN1300297C (en) * 2002-09-20 2007-02-14 华东理工大学 Digestive reactor for animal cell extension inoculation
RU2372924C2 (en) 2003-02-20 2009-11-20 Ситори Терапьютикс,Инк Application of composition consisting of concentrated cell population representing stem cells and undifferentiated precursors
JP2007524396A (en) 2003-06-25 2007-08-30 サイトリ セラピューティクス インコーポレイテッド System and method for separating and concentrating regenerative cells from tissue
CA2539346A1 (en) 2003-09-17 2005-04-21 Macropore Biosurgery, Inc. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
CA2544498A1 (en) * 2003-11-03 2005-05-12 Centocor, Inc. Method for maintaining low shear in a bioprocessing system
EP1743021B1 (en) 2004-03-19 2014-03-26 Cytori Therapeutics, Inc. Method of preparing regenerative cells on a cell carrier and cell carrier containment device
US20050244957A1 (en) * 2004-04-29 2005-11-03 Healthy Soils, Inc. Regenerating tank
EP2980206A1 (en) 2004-07-01 2016-02-03 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
EP1778833B1 (en) 2004-07-01 2011-03-09 Cytori Therapeutics, Inc. Methods of using regenerative cells to promote wound healing
US7368279B2 (en) * 2004-11-24 2008-05-06 The Regents Of The University Of Michigan Three dimensional bioengineered smooth muscle tissue and sphincters and methods therefor
AU2005332046B2 (en) 2005-05-25 2013-05-16 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
ATE459701T1 (en) * 2005-12-30 2010-03-15 Drugmode Aps BIOREACTOR FOR CELL AND TISSUE CULTURE
ATE531792T1 (en) * 2006-03-30 2011-11-15 Mark Clarke THREE-DIMENSIONAL MINERALIZED BONE CONSTRUCTS
KR100761922B1 (en) 2006-04-07 2007-10-04 양현진 Cell culture method by regulating signal between cells in cell culture using powder support
US9057044B2 (en) 2006-08-30 2015-06-16 Meir Israelowitz Laminar flow reactor
US20080138828A1 (en) * 2006-12-08 2008-06-12 Marshall University Research Corporation Microgravity bioreactor systems for production of bioactive compounds and biological macromolecules
US20110033887A1 (en) * 2007-09-24 2011-02-10 Fang Nicholas X Three-Dimensional Microfabricated Bioreactors with Embedded Capillary Network
US8993231B2 (en) * 2008-03-18 2015-03-31 Marshall University Research Corporation Methods for stem cell production and therapy
WO2010021993A1 (en) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
US9316632B2 (en) 2009-03-17 2016-04-19 Marshall University Research Corporation Methods of screening chemotherapeutic agents and treating cancer
US9133431B2 (en) 2009-05-01 2015-09-15 Bimini Technologies Llc Systems, methods and compositions for optimizing tissue and cell enriched grafts
US9862923B2 (en) 2010-03-26 2018-01-09 Philip Morris Usa Inc. Cultured tobacco cells as a matrix for consumable products
US9592255B2 (en) 2010-07-01 2017-03-14 The Regents Of The University Of Michigan Scaffold-free three dimensional nerve fibroblast constructs
WO2017117229A1 (en) 2015-12-30 2017-07-06 Wake Forest University Health Sciences Tissue-engineered gut-sphincter complexes and methods of making the same
US11311367B2 (en) 2012-01-31 2022-04-26 Wake Forest University Health Sciences Tissue-engineered gut-sphincter complexes and methods of making the same
WO2014152906A2 (en) 2013-03-14 2014-09-25 Research Institute At Nationwide Children's Hospital, Inc. Tissue engineered intestine
ES2755741T3 (en) * 2013-07-02 2020-04-23 Miltenyi Biotec Bv & Co Kg Centrifugation chamber with layers of gas-permeable membranes for cell culture
US20150017714A1 (en) 2013-07-02 2015-01-15 Miltenyi Biotec Gmbh Centrifugation chamber with gas permeable membrane layers for cell cultivation
EP2821144A1 (en) * 2013-07-02 2015-01-07 Miltenyi Biotec GmbH Centrifugation chamber with gas-permeable membrane for cell cultivation
US20150182558A1 (en) * 2014-01-02 2015-07-02 PSC Cosmetics Ltd. Method and apparatus for harvesting, creating and implanting a fibrin clot biomaterial
CN111100790B (en) * 2019-12-31 2022-09-02 黄山市中新生物科技有限公司 Cell culture bioreactor

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1369593A (en) * 1970-09-17 1974-10-09 Wellcome Found Culture apparatus
CH563456A5 (en) * 1972-08-18 1975-06-30 Mueller Hans Maennedorf
IT1097442B (en) * 1977-08-18 1985-08-31 Guigan Jean CONDITIONING DEVICE OF A LIQUID SAMPLE IN PREPARATION OF ITS ANALYSIS
AU6156780A (en) * 1979-08-24 1981-04-09 G.D. Searle & Co. Stack plate culture
CH647547A5 (en) * 1982-02-12 1985-01-31 Chemap Ag METHOD AND DEVICE FOR BREEDING MICROORGANISMS.
DE3600487A1 (en) * 1986-01-10 1987-07-16 Gebhardt Rolf PERIFUSION DEVICE FOR BREEDING LIVING CELLS AND METHOD FOR PERIFUSION CULTURE
US4846786A (en) * 1987-04-30 1989-07-11 Massachusetts Institute Of Technology Bioreactor containing suspended, immobilized species
US5605835A (en) * 1988-05-23 1997-02-25 Regents Of The University Of Minnesota Bioreactor device with application as a bioartificial liver
US5153132A (en) * 1988-06-30 1992-10-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Three-dimensional co-culture process
US5153131A (en) * 1990-12-11 1992-10-06 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration High aspect reactor vessel and method of use
US5026650A (en) * 1988-06-30 1991-06-25 The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration Horizontally rotated cell culture system with a coaxial tubular oxygenator
US4988623A (en) * 1988-06-30 1991-01-29 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Rotating bio-reactor cell culture apparatus
US5308764A (en) * 1988-06-30 1994-05-03 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Multi-cellular, three-dimensional living mammalian tissue
CA1307225C (en) * 1988-07-19 1992-09-08 David W. Armstrong Cell culture bioreactor
US5151368A (en) * 1991-01-11 1992-09-29 Technical Research Associates, Inc. Dual axis, continuous flow bioreactor apparatus
US5476783A (en) * 1992-03-23 1995-12-19 Koch Engineering Company, Inc. Bioreactor method of culturing and propagating cells with a woven motionless mixing element
US5330908A (en) * 1992-12-23 1994-07-19 The United States Of America As Represented By The Administrator, National Aeronautics And Space Administration High density cell culture system
US5686301A (en) * 1993-09-02 1997-11-11 Heraeus Instruments Gmbh Culture vessel for cell cultures
US5763279A (en) * 1993-09-09 1998-06-09 Synthecon, Inc. Gas permeable bioreactor and method of use
US5437998A (en) * 1993-09-09 1995-08-01 Synthecon, Inc. Gas permeable bioreactor and method of use
US5702941A (en) * 1993-09-09 1997-12-30 Synthecon, Inc. Gas permeable bioreactor and method of use
US5622857A (en) * 1995-08-08 1997-04-22 Genespan Corporation High performance cell culture bioreactor and method
US5688687A (en) * 1995-06-07 1997-11-18 Aastrom Biosciences, Inc. Bioreactor for mammalian cell growth and maintenance
US5523228A (en) * 1995-07-07 1996-06-04 Hmri/Clmf Hydrodynamic cell culture environment for three dimensional tissue growth
US5928936A (en) * 1997-04-09 1999-07-27 Huntington Medical Research Institutes Cell culture container that self-seals after cannula penetration made of porous sheets

Also Published As

Publication number Publication date
WO2000000586A1 (en) 2000-01-06
US6001642A (en) 1999-12-14
AU4965799A (en) 2000-01-17
CA2336062C (en) 2009-04-07
EP1093513A1 (en) 2001-04-25
EP1093513A4 (en) 2002-04-24
WO2000000586A9 (en) 2000-12-14

Similar Documents

Publication Publication Date Title
CA2336062A1 (en) Bioreactor and cell culturing processes using the bioreactor
CN1039432C (en) Cell culture insert
US4939151A (en) Adherent cell culture flask
Wada et al. Continuous production of ethanol using immobilized growing yeast cells
CN1039433C (en) Cell culture insert
CA2329815A1 (en) Solid state fermenter and procedure for solid state fermentation
US5955344A (en) Apparatus and method for growing anaerobic microorganisms
DE3661273D1 (en) Method and apparatus for carrying out cell culture
EP1245670A3 (en) Method and apparatus for culturing cells
EP0790300B1 (en) Culture vessel and assembly
CA2058930A1 (en) Media for cell growth and method for making them
JPS62171686A (en) Production of biological group composite
Ogawa et al. Production of kojic acid by membrane-surface liquid culture of Aspergillus oryzae NRRL484
AU2003261308A1 (en) Method for preparing cell cultures from biological specimens for chemotherapeutic and other assays
Wakisaka et al. Development of a cylindrical apparatus for membrane-surface liquid culture and production of kojic acid using Aspergillus oryzae NRRL484
Ogawa et al. Production of neutral protease by membrane-surface liquid culture of Aspergillus oryzae IAM2704
US6210959B1 (en) Apparatus for the cultivation and concentration of non-adherent cells as well as for co-cultivation of two different cell species
WO2002042419A3 (en) Cell culture apparatus and methods of use
WO2001075072A3 (en) Production of TcR gamma delta T cells
CN211420137U (en) Bottle stopper for strain culture
Tiltscher et al. Immobilization of the slime mould Dictyostelium discoideum for the continuous production of recombinant human antithrombin III
Wang et al. Penicillin fermentation in a 200-liter tower fermentor using cells confined to microbeads
CA2214384A1 (en) Compartmentalized tissue culture bag
CN223752812U (en) Culture bottle convenient for liquid replacement
CN221319952U (en) Micro-reactor bottle

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20190628