CA2330570A1 - Enzyme d'acide nucleique de coupure d'un arn - Google Patents

Enzyme d'acide nucleique de coupure d'un arn Download PDF

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Publication number
CA2330570A1
CA2330570A1 CA002330570A CA2330570A CA2330570A1 CA 2330570 A1 CA2330570 A1 CA 2330570A1 CA 002330570 A CA002330570 A CA 002330570A CA 2330570 A CA2330570 A CA 2330570A CA 2330570 A1 CA2330570 A1 CA 2330570A1
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Canada
Prior art keywords
enzyme
substrate
nucleotide
cleavage site
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002330570A
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English (en)
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CA2330570C (fr
Inventor
Jean-Pierre Perreault
Sirinart Ananvoranich
Daniel Lafontaine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SOCPRA Sciences Sante et Humaines sec
Original Assignee
Universite De Sherbrooke
Jean-Pierre Perreault
Sirinart Ananvoranich
Daniel Lafontaine
Societe De Commercialisation Des Produits De La Recherche Appliquee - So Cpra Sciences Sante Et Humaines
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Publication date
Priority claimed from CA002230203A external-priority patent/CA2230203A1/fr
Application filed by Universite De Sherbrooke, Jean-Pierre Perreault, Sirinart Ananvoranich, Daniel Lafontaine, Societe De Commercialisation Des Produits De La Recherche Appliquee - So Cpra Sciences Sante Et Humaines filed Critical Universite De Sherbrooke
Priority to CA002330570A priority Critical patent/CA2330570C/fr
Publication of CA2330570A1 publication Critical patent/CA2330570A1/fr
Application granted granted Critical
Publication of CA2330570C publication Critical patent/CA2330570C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/123—Hepatitis delta

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé de coupure d'un substrat d'acide nucléique par une enzyme d'acide nucléique en un site de coupure consistant à mélanger le substrat à l'enzyme. Le substrat comporte au niveau du site de coupure une séquence d'au moins 7 nucléotides dont au moins 6 nucléotides 3', et un nucléotide 5' de formule 5'-H' &darr& GNNHNN-3', dans laquelle: chaque N est un nucléotide identique ou différent; H est un nucléotide choisi parmi A, U, C et T; &darr& est le site de coupure; H' est un ribonucléotide choisi parmi A, U et C, et au sujet de laquelle: (i) le premier nucléotide 3' du site de coupure peut former une paire flottante avec l'enzyme, (ii) les deuxième, troisième, cinquième et sixième nucléotides 3' du site de coupure peuvent former avec l'enzyme des paires de base de Watson-Crick conventionnelles, (iii) le quatrième nucléotide 3' du site de coupure peut former avec l'enzyme des paires de base de Watson-Crick non conventionnelles, et (iv) le premier nucléotide 5' du site de coupure ne peut former de paire de base avec l'enzyme; de plus, l'enzyme comporte une portion se fixant au substrat et capable de former une paire de base avec les six nucléotide 3' du site de coupure du substrat, ladite portion comportant la séquence 3'-UNNXNN-5' dans laquelle chaque N est un nucléotide identique ou différent; X est un nucléotide choisi parmi T, U, A et G et qui lorsque le substrat s'y fixe entraîne la coupure du substrat au site de coupure.
CA002330570A 1998-04-29 1999-04-29 Enzyme d'acide nucleique de coupure d'un arn Expired - Fee Related CA2330570C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002330570A CA2330570C (fr) 1998-04-29 1999-04-29 Enzyme d'acide nucleique de coupure d'un arn

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA2,230,203 1998-04-29
CA002230203A CA2230203A1 (fr) 1998-04-29 1998-04-29 Ribozyme delta pour le clivage de l'arn
CA002330570A CA2330570C (fr) 1998-04-29 1999-04-29 Enzyme d'acide nucleique de coupure d'un arn
PCT/CA1999/000391 WO1999055856A2 (fr) 1998-04-29 1999-04-29 Enzyme d'acide nucleique de coupure d'un arn

Publications (2)

Publication Number Publication Date
CA2330570A1 true CA2330570A1 (fr) 1999-11-04
CA2330570C CA2330570C (fr) 2010-01-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002330570A Expired - Fee Related CA2330570C (fr) 1998-04-29 1999-04-29 Enzyme d'acide nucleique de coupure d'un arn

Country Status (1)

Country Link
CA (1) CA2330570C (fr)

Also Published As

Publication number Publication date
CA2330570C (fr) 2010-01-12

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