CA2330570A1 - Enzyme d'acide nucleique de coupure d'un arn - Google Patents
Enzyme d'acide nucleique de coupure d'un arn Download PDFInfo
- Publication number
- CA2330570A1 CA2330570A1 CA002330570A CA2330570A CA2330570A1 CA 2330570 A1 CA2330570 A1 CA 2330570A1 CA 002330570 A CA002330570 A CA 002330570A CA 2330570 A CA2330570 A CA 2330570A CA 2330570 A1 CA2330570 A1 CA 2330570A1
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- substrate
- nucleotide
- cleavage site
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title claims abstract 47
- 108090000790 Enzymes Proteins 0.000 title claims abstract 47
- 238000003776 cleavage reaction Methods 0.000 title claims abstract 32
- 230000007017 scission Effects 0.000 title claims abstract 32
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 20
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 20
- 239000000758 substrate Substances 0.000 claims abstract 40
- 239000002773 nucleotide Substances 0.000 claims abstract 36
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 36
- 238000000034 method Methods 0.000 claims abstract 17
- 108091028664 Ribonucleotide Proteins 0.000 claims abstract 13
- 239000002336 ribonucleotide Substances 0.000 claims abstract 13
- 125000002652 ribonucleotide group Chemical group 0.000 claims abstract 13
- 239000005547 deoxyribonucleotide Substances 0.000 claims 4
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 4
- 239000000203 mixture Substances 0.000 claims 4
- 102000014914 Carrier Proteins Human genes 0.000 claims 2
- 241000724709 Hepatitis delta virus Species 0.000 claims 2
- 108091008324 binding proteins Proteins 0.000 claims 2
- 239000002719 pyrimidine nucleotide Substances 0.000 claims 2
- 150000003230 pyrimidines Chemical class 0.000 claims 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
- C12N2310/123—Hepatitis delta
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention porte sur un procédé de coupure d'un substrat d'acide nucléique par une enzyme d'acide nucléique en un site de coupure consistant à mélanger le substrat à l'enzyme. Le substrat comporte au niveau du site de coupure une séquence d'au moins 7 nucléotides dont au moins 6 nucléotides 3', et un nucléotide 5' de formule 5'-H' &darr& GNNHNN-3', dans laquelle: chaque N est un nucléotide identique ou différent; H est un nucléotide choisi parmi A, U, C et T; &darr& est le site de coupure; H' est un ribonucléotide choisi parmi A, U et C, et au sujet de laquelle: (i) le premier nucléotide 3' du site de coupure peut former une paire flottante avec l'enzyme, (ii) les deuxième, troisième, cinquième et sixième nucléotides 3' du site de coupure peuvent former avec l'enzyme des paires de base de Watson-Crick conventionnelles, (iii) le quatrième nucléotide 3' du site de coupure peut former avec l'enzyme des paires de base de Watson-Crick non conventionnelles, et (iv) le premier nucléotide 5' du site de coupure ne peut former de paire de base avec l'enzyme; de plus, l'enzyme comporte une portion se fixant au substrat et capable de former une paire de base avec les six nucléotide 3' du site de coupure du substrat, ladite portion comportant la séquence 3'-UNNXNN-5' dans laquelle chaque N est un nucléotide identique ou différent; X est un nucléotide choisi parmi T, U, A et G et qui lorsque le substrat s'y fixe entraîne la coupure du substrat au site de coupure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002330570A CA2330570C (fr) | 1998-04-29 | 1999-04-29 | Enzyme d'acide nucleique de coupure d'un arn |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA002230203A CA2230203A1 (fr) | 1998-04-29 | 1998-04-29 | Ribozyme delta pour le clivage de l'arn |
| CA2,230,203 | 1998-04-29 | ||
| PCT/CA1999/000391 WO1999055856A2 (fr) | 1998-04-29 | 1999-04-29 | Enzyme d'acide nucleique de coupure d'un arn |
| CA002330570A CA2330570C (fr) | 1998-04-29 | 1999-04-29 | Enzyme d'acide nucleique de coupure d'un arn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2330570A1 true CA2330570A1 (fr) | 1999-11-04 |
| CA2330570C CA2330570C (fr) | 2010-01-12 |
Family
ID=25680061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002330570A Expired - Fee Related CA2330570C (fr) | 1998-04-29 | 1999-04-29 | Enzyme d'acide nucleique de coupure d'un arn |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2330570C (fr) |
-
1999
- 1999-04-29 CA CA002330570A patent/CA2330570C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2330570C (fr) | 2010-01-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |