CA2326847A1 - Preparation d'adenovirus recombines portant un gene rep de virus adeno-associe - Google Patents

Preparation d'adenovirus recombines portant un gene rep de virus adeno-associe Download PDF

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Publication number
CA2326847A1
CA2326847A1 CA002326847A CA2326847A CA2326847A1 CA 2326847 A1 CA2326847 A1 CA 2326847A1 CA 002326847 A CA002326847 A CA 002326847A CA 2326847 A CA2326847 A CA 2326847A CA 2326847 A1 CA2326847 A1 CA 2326847A1
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CA
Canada
Prior art keywords
cells
helper
aav
rep gene
rep
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002326847A
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English (en)
Inventor
Gennaro Ciliberto
Stefano Colloca
Nicola La Monica
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istituto di Ricerche di Biologia Molecolare P Angeletti SpA
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IT1998/000082 external-priority patent/WO1998045462A1/fr
Application filed by Individual filed Critical Individual
Publication of CA2326847A1 publication Critical patent/CA2326847A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne la production de particules adénovirales par des cellules d'incubation contenant un vecteur d'adénovirus auxiliaire et un vecteur adénoviral dépendant du virus auxiliaire incluant un gène rep de virus adéno-associé (AAV), tel que rep78. L'invention concerne des cellules contenant un vecteur d'adénovirus auxiliaire. Un vecteur adénoviral dépendant du virus auxiliaire qui inclut un gène rep d'AAV est introduit dans les cellules, par exemple par infection à l'aide de particules virales infectieuses. Les cellules sont incubées de manière à produire des particules adénovirales contenant un acide nucléique qui inclut le gène rep d'AAV. Il est avantageux de préincuber les cellules contenant le vecteur d'adénovirus auxiliaire pendant 0,5-12 heures, de préférence environ 4 heures, pour permettre l'expression de protéines virales nécessaires à la réplication du génome adénoviral avant d'introduire le vecteur d'adénovirus dépendant du virus auxiliaire qui inclut un gène rep d'AAV.
CA002326847A 1998-04-08 1999-04-08 Preparation d'adenovirus recombines portant un gene rep de virus adeno-associe Abandoned CA2326847A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
PCT/IT1998/000082 WO1998045462A1 (fr) 1997-04-08 1998-04-08 Vecteurs de recombinaison derives d'un virus associe a un adenovirus adaptes a la therapie genique
ITPCT/IT98/00082 1998-04-08
GB9813670.8 1998-06-24
GBGB9813670.8A GB9813670D0 (en) 1998-04-08 1998-06-24 Preparation of recombinant adenovirus carrying a rep gene of adeno-associated virus
PCT/EP1999/002384 WO1999053084A1 (fr) 1998-04-08 1999-04-08 Preparation d'adenovirus recombines portant un gene rep de virus adeno-associe

Publications (1)

Publication Number Publication Date
CA2326847A1 true CA2326847A1 (fr) 1999-10-21

Family

ID=26313926

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002326847A Abandoned CA2326847A1 (fr) 1998-04-08 1999-04-08 Preparation d'adenovirus recombines portant un gene rep de virus adeno-associe

Country Status (5)

Country Link
EP (1) EP1068343A1 (fr)
AU (1) AU752811B2 (fr)
CA (1) CA2326847A1 (fr)
GB (1) GB9813670D0 (fr)
WO (1) WO1999053084A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19905501B4 (de) * 1999-02-10 2005-05-19 MediGene AG, Gesellschaft für molekularbiologische Kardiologie und Onkologie Verfahren zur Herstellung eines rekombinanten Adeno-assoziierten Virus, geeignete Mittel hierzu sowie Verwendung zur Herstellung eines Arzneimittels
US6627617B1 (en) 1999-10-01 2003-09-30 University Of North Carolina At Chapel Hill Temperature-sensitive regulation of viral vector production

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5856152A (en) * 1994-10-28 1999-01-05 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV vector and methods of use therefor
CA2257148A1 (fr) * 1996-05-31 1997-12-04 Wei-Wei Zhang Vecteur mini-adenoviral

Also Published As

Publication number Publication date
WO1999053084A1 (fr) 1999-10-21
AU752811B2 (en) 2002-10-03
AU3926599A (en) 1999-11-01
EP1068343A1 (fr) 2001-01-17
GB9813670D0 (en) 1998-08-26

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued