CA2323756A1 - Genetic engineering salt tolerance in crop plants - Google Patents
Genetic engineering salt tolerance in crop plants Download PDFInfo
- Publication number
- CA2323756A1 CA2323756A1 CA002323756A CA2323756A CA2323756A1 CA 2323756 A1 CA2323756 A1 CA 2323756A1 CA 002323756 A CA002323756 A CA 002323756A CA 2323756 A CA2323756 A CA 2323756A CA 2323756 A1 CA2323756 A1 CA 2323756A1
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- Prior art keywords
- polypeptide
- nucleic acid
- acid molecule
- seq
- transporter
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
Abstract
The invention is isolated nucleic acid molecules encoding Na+/H+ exchanger polypeptides for extrusion of monovalent cations (preferably lithium ions and potassium ions, most preferably sodium ions) from the cytosol of cells to provide the cell with increased salt tolerance. In a preferred embodiment, the nucleic acid is obtained from Arabidopsis thaliana. Crop species transformed with the nucleic acid molecule are capable of surviving in soil with high salt levels that would normally inhibit growth of the crop species.
Claims (14)
1. An isolated nucleic acid molecule encoding a PNHX transporter polypeptide, or a fragment of a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
2. An isolated nucleic acid molecule encoding a TNHX transporter polypeptide, PNHX transporter polypeptide, or a fragment of a polypeptide having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell, comprising a nucleic acid molecule selected from the group consisting of:
(a) a nucleic acid molecule that hybridizes to all or part of a nucleic acid molecule shown in [SEQ ID NO:1], [SEQ ID NO:3], [SEQ ID NO:17], [SEQ ID NO:19], or a complement thereof under moderate or high stringency hybridization conditions, wherein the nucleic acid molecule encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell;
(b) a nucleic acid molecule degenerate with respect to (a), wherein the nucleic molecule encodes a TNHX transporter polypeptide, a PNHX
transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
transporter activity and capable of increasing salt tolerance in a cell, comprising a nucleic acid molecule selected from the group consisting of:
(a) a nucleic acid molecule that hybridizes to all or part of a nucleic acid molecule shown in [SEQ ID NO:1], [SEQ ID NO:3], [SEQ ID NO:17], [SEQ ID NO:19], or a complement thereof under moderate or high stringency hybridization conditions, wherein the nucleic acid molecule encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell;
(b) a nucleic acid molecule degenerate with respect to (a), wherein the nucleic molecule encodes a TNHX transporter polypeptide, a PNHX
transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
3. The nucleic acid molecule of claim 2, wherein the hybridization conditions comprise moderate or high stringency conditions selected from conditions about those in Table 4.
4. An isolated nucleic acid molecule encoding a TNHX transporter polypeptide or a PNHX transporter polypeptide, or a fragment of a polypeptide having Na+/H+
transporter activity and capable of increasing salt tolerances in a cell, comprising a nucleic acid molecule selected from the group consisting of:
(a) the nucleic acid molecule of the coding strand shown in [SEQ ID NO:1], [SEQ ID NO:3], [SEQ ID NO:17], [SEQ ID NO:19] or a complement thereof;
(b) a nucleic acid molecule encoding the same amino acid sequence as a nucleotide sequence of (a); and (c) a nucleic acid molecule having at least 17% identity with the nucleotide sequence of (a) and which encodes a TNHX transporter polypeptide or the PNHX transporter polypeptide or a polypeptide having Na+/H+
transporter activity.
transporter activity and capable of increasing salt tolerances in a cell, comprising a nucleic acid molecule selected from the group consisting of:
(a) the nucleic acid molecule of the coding strand shown in [SEQ ID NO:1], [SEQ ID NO:3], [SEQ ID NO:17], [SEQ ID NO:19] or a complement thereof;
(b) a nucleic acid molecule encoding the same amino acid sequence as a nucleotide sequence of (a); and (c) a nucleic acid molecule having at least 17% identity with the nucleotide sequence of (a) and which encodes a TNHX transporter polypeptide or the PNHX transporter polypeptide or a polypeptide having Na+/H+
transporter activity.
5. The nucleic acid molecule of any of claims 1 to 4, wherein the TNHX
transporter polypeptide or the PNHX transporter polypeptide comprises an AtNHX
transporter polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
transporter polypeptide or the PNHX transporter polypeptide comprises an AtNHX
transporter polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
6. The nucleic acid molecule of claim 1, comprising all or part of a nucleotide sequence shown in [SEQ ID NO:1], [SEQ ID NO:3], [SEQ ID NO:17], [SEQ ID
NO:19], or a complement thereof.
NO:19], or a complement thereof.
7. An AtNHX nucleic acid molecule isolated from Arabidopsis thaliana, or a fragment thereof encoding a transporter polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
8. A recombinant nucleic acid molecule comprising a nucleic acid molecule of any of claims 1 to 4 and a constitutive promoter sequence or an inducible promoter sequence, operatively linked so that the promoter enhances transcription of the nucleic acid molecule in a host cell.
9. The nucleic acid molecule of claim 6, wherein the molecule comprises genomic DNA, cDNA or RNA.
10. The nucleic acid molecule of claim 6, wherein the nucleic acid molecule is chemically synthesized.
11. The nucleic acid molecule of claim 6, wherein the nucleic acid molecule is isolated from Arabidopsis thaliana.
12. The nucleic acid molecule of any of claims 1 to 4, wherein the TNHX
transporter polypeptide or the PNHX transporter polypeptide is capable of extruding monovalent cations out of the cytosol of a cell to provide the cell with increased salt tolerance, wherein the monovalent canons are selected from at least one of the group consisting of sodium, lithium and potassium.
transporter polypeptide or the PNHX transporter polypeptide is capable of extruding monovalent cations out of the cytosol of a cell to provide the cell with increased salt tolerance, wherein the monovalent canons are selected from at least one of the group consisting of sodium, lithium and potassium.
13. The nucleic acid molecule of claim 12, wherein the cell comprises a plant cell.
14. The nucleic acid molecule of claim 13, wherein the monovalent rations are extruded into a vacuole or into the extracellular space.
16. The nucleic acid molecule of claim 18, wherein the monovalent cations are extruded into a vacuole or into the extracellular space.
17. An isolated nucleic acid molecule comprising a nucleic acid molecule selected from the group consisting of 8 to 10 nucleotides of the nucleic acid molecule of claim 7,11 to 25 nucleotides of the nucleic acid molecule of claim 8 and 26 to nucleotides of the nucleic acid molecule of claim 8.
18. An Isolated oligonucleotide comprising at least about 10 consecutive nucleotides from a sequence selected from the group consisting of 5'-GCCATGTTGGATTCTCTAGTGTCG-3 [SEQ ID NO: 11], 6'-CCGAATTCTCAAAGCTTTTCTTCCACG-3' [SEQ ID NO:12], 5'-CGGAATTCACAGAAAAACACAGTGAGGAT-3' [SEQ ID NO:13], 5'-GCGATGTTGGATTCTCTAGTGTCG-3 [SEQ ID NO:14], 5'-CGGAATTCTCAAAGCTTTTCTTCCAGG-3' [SEQ ID NO:15] and 5'-CGGAATTGACAGAAAAACACAGTGAGGAT-3' [SEQ ID NO:16], with the provisio that the oligonucleotide is not gcctactgtagtaaaggaaataacttcacagaaaaactaaacagaagcat.
19. The isolated oligonucleotide of claim 18, wherein the oligonucleotide comprises at least 15 consecutive nucleotides.
20. A vector comprising the nucleic acid molecule of any of claims 1 to 4.
21. The vector of claim 20, comprising a promoter selected from the group consisting of a super promoter, a 35S promofier of cauliflower mosaic virus, a drought-inducible promoter, an ABA-inducible promoter, a heat shock-inducible promoter, a salt-inducible promoter, a copper-inducible promoter, a steroid-inducible promoter and a tissue-specific promoter.
22. A host cell comprising the recombinant nucleic acid molecule of claim 10 or the vector of claim 20, or progeny of the host cell.
23. The host cell of claim 22, selected from the group consisting of a fungal cell, a yeast cell, a bacterial cell, a microorganism cell and a plant cell.
24. The nucleic acid molecule, vector or host cell of any of claims 1 to 16 or 20 to 23, wherein the polypeptide comprises a vacuolar antiport.
25. The nucleic acid molecule, vector or host cell of any of claims 1 to 16 or 20 to 23, wherein the polypeptide comprises 250 amino acids or longer.
39. An isolated PNHX transporter polypeptide or a fragment thereof having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell.
40. The polypeptide of claim 39 comprising an AtNHX transporter polypeptide.
41. The polypeptide of claim 40 comprising all or part of an amino acid sequence in [SEQ ID NO:2], [SEQ ID NO:4], [SEQ ID NO:18] or [SEQ ID NO:20].
42. A polypeptide fragment of the AtNHX transporter polypeptide of claim 40, or a peptide mimetic of the AtNHX transporter polypeptide, having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell.
43. The polypeptide fragment of claim 42, consisting of at least 20 amino acids, which fragment has Na+/H+ transporter activity and is capable of increasing salt tolerance in a cell.
44. The fragment or peptide mimetic of claim 42, which is capable of being bound by an antibody to the polypeptide of claim 41.
45. The polypeptide of claim 39 which is recombinantly produced.
46. An isolated and purified transporter polypeptide comprising the amino acid sequence of a TNHX transporter polypeptide or a PNHX transporter polypeptide, wherein the transporter polypeptide is encoded by a nucleic acid molecule that hybridizes under moderate or stringent conditions to a nucleic acid molecule in [SEQ ID NO:1], [SEQ ID NO:3], (SEQ ID NO:17], [SEQ ID NO:19], a degenerate form thereof or a complement.
47. A polypeptide comprising a sequence having greater than 40% sequence identity to the polypeptide of claim 41.
48. The polypeptide of claim 40, wherein the polypeptide comprises a Na+/H+
transporter polypeptide.
49. The polypeptide of Claim 48, isolated from Arabidopsis thaliana.
50. An isolated nucleic acid molecule encoding the polypeptide of claims 38, 40 or 41.
51. An antibody directed against the polypeptide of claim 41.
52. The antibody of claim 51, comprising a monoclonal antibody or a polyclonal antibody, 53. An isolated nucleic acid molecule encoding a TNHX transporter polypeptide or a PNHX transporter polypeptide, or a fragment of a polypeptide having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell, comprising a nucleic acid molecule selected from the group consisting of (a) a nucleic acid molecule that hybridizes to all or part of a nucleic molecule in [SEQ ID NO:5], [SEQ ID NO:7], [SEQ ID NO:9] or to a nucleic acid molecule comprising about nucleotides 1-1487 of [SEQ ID
NO:9], or a complement thereof under moderate or high stringency hybridization conditions, wherein the nucleic acid molecule encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell;
(b) a nucleic add molecule degenerate with respect to (a), wherein the nucleic molecule encodes a TNHX polypeptide, a PNHX polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
(c) the nucleic acid molecule of the coding strand shown in [SEQ ID
NO:5], [SEQ ID NO:7], [SEQ ID NO:9], nucleotides 1-1487 of [SEQ ID
NO:9], or an isolated nucleic acid molecule including about 1614 nucleic acids including [SEQ ID NO:5], [SEQ ID NO:7], nucleotides 1 to 1487 of the nucleic add molecule in [SEQ ID NO:9] or the complement thereof;
(d) a nucleic acid molecule encoding the same amino and sequence as a nucleotide sequence of (c); and (e) a nucleic acid molecule having of least 17% sequence identity with the nucleotide sequence of (e) and which encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
54. A polypeptide produced from the nucleic acid molecule of claim 53.
55. A polypeptide comprising (a) the amino acid sequence in [SEQ ID NO:6], [SEQ
ID NO:8], [SEQ ID NO:10] (b) amino acids 1 to 496 of [SEQ ID NO:10]; and (c) a sequence having greater than 28% homology to the polypeptide in (a) or (b).
56. The polypeptide of claim 55, wherein the polypeptide comprises a Na+/H+
transporter polypeptide capable of increasing salt tolerance in a Cell.
57. A DNA molecule encoding the polypeptide of claim 55.
58. A method of producing a genetically transformed plant which expresses or overexpressss a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell and wherein the plant has increased salt tolerance, comprising:
(a) cloning or synthesizing a TNHX nucleic acid molecule, a PNHX
nucleic acid molecule or a nucleic acid molecule which codes for a Na+/H+ transporter polypeptide, wherein the potypeptide is capable of providing salt tolerance to a plant;
(d) inserting the nucleic acid molecule in a vector so that the nucleic acid molecule is operably linked to a promoter;
(c) inserting the vector into a plant cell or plant seed;
(d) regenerating the plant from the plant cell or plant seed, wherein salt tolerance in the plant is increased compared to a wild type plant.
58. A transgenic plant produced according to the method of claim 57.
16. The nucleic acid molecule of claim 18, wherein the monovalent cations are extruded into a vacuole or into the extracellular space.
17. An isolated nucleic acid molecule comprising a nucleic acid molecule selected from the group consisting of 8 to 10 nucleotides of the nucleic acid molecule of claim 7,11 to 25 nucleotides of the nucleic acid molecule of claim 8 and 26 to nucleotides of the nucleic acid molecule of claim 8.
18. An Isolated oligonucleotide comprising at least about 10 consecutive nucleotides from a sequence selected from the group consisting of 5'-GCCATGTTGGATTCTCTAGTGTCG-3 [SEQ ID NO: 11], 6'-CCGAATTCTCAAAGCTTTTCTTCCACG-3' [SEQ ID NO:12], 5'-CGGAATTCACAGAAAAACACAGTGAGGAT-3' [SEQ ID NO:13], 5'-GCGATGTTGGATTCTCTAGTGTCG-3 [SEQ ID NO:14], 5'-CGGAATTCTCAAAGCTTTTCTTCCAGG-3' [SEQ ID NO:15] and 5'-CGGAATTGACAGAAAAACACAGTGAGGAT-3' [SEQ ID NO:16], with the provisio that the oligonucleotide is not gcctactgtagtaaaggaaataacttcacagaaaaactaaacagaagcat.
19. The isolated oligonucleotide of claim 18, wherein the oligonucleotide comprises at least 15 consecutive nucleotides.
20. A vector comprising the nucleic acid molecule of any of claims 1 to 4.
21. The vector of claim 20, comprising a promoter selected from the group consisting of a super promoter, a 35S promofier of cauliflower mosaic virus, a drought-inducible promoter, an ABA-inducible promoter, a heat shock-inducible promoter, a salt-inducible promoter, a copper-inducible promoter, a steroid-inducible promoter and a tissue-specific promoter.
22. A host cell comprising the recombinant nucleic acid molecule of claim 10 or the vector of claim 20, or progeny of the host cell.
23. The host cell of claim 22, selected from the group consisting of a fungal cell, a yeast cell, a bacterial cell, a microorganism cell and a plant cell.
24. The nucleic acid molecule, vector or host cell of any of claims 1 to 16 or 20 to 23, wherein the polypeptide comprises a vacuolar antiport.
25. The nucleic acid molecule, vector or host cell of any of claims 1 to 16 or 20 to 23, wherein the polypeptide comprises 250 amino acids or longer.
39. An isolated PNHX transporter polypeptide or a fragment thereof having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell.
40. The polypeptide of claim 39 comprising an AtNHX transporter polypeptide.
41. The polypeptide of claim 40 comprising all or part of an amino acid sequence in [SEQ ID NO:2], [SEQ ID NO:4], [SEQ ID NO:18] or [SEQ ID NO:20].
42. A polypeptide fragment of the AtNHX transporter polypeptide of claim 40, or a peptide mimetic of the AtNHX transporter polypeptide, having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell.
43. The polypeptide fragment of claim 42, consisting of at least 20 amino acids, which fragment has Na+/H+ transporter activity and is capable of increasing salt tolerance in a cell.
44. The fragment or peptide mimetic of claim 42, which is capable of being bound by an antibody to the polypeptide of claim 41.
45. The polypeptide of claim 39 which is recombinantly produced.
46. An isolated and purified transporter polypeptide comprising the amino acid sequence of a TNHX transporter polypeptide or a PNHX transporter polypeptide, wherein the transporter polypeptide is encoded by a nucleic acid molecule that hybridizes under moderate or stringent conditions to a nucleic acid molecule in [SEQ ID NO:1], [SEQ ID NO:3], (SEQ ID NO:17], [SEQ ID NO:19], a degenerate form thereof or a complement.
47. A polypeptide comprising a sequence having greater than 40% sequence identity to the polypeptide of claim 41.
48. The polypeptide of claim 40, wherein the polypeptide comprises a Na+/H+
transporter polypeptide.
49. The polypeptide of Claim 48, isolated from Arabidopsis thaliana.
50. An isolated nucleic acid molecule encoding the polypeptide of claims 38, 40 or 41.
51. An antibody directed against the polypeptide of claim 41.
52. The antibody of claim 51, comprising a monoclonal antibody or a polyclonal antibody, 53. An isolated nucleic acid molecule encoding a TNHX transporter polypeptide or a PNHX transporter polypeptide, or a fragment of a polypeptide having Na+/H+
transporter activity and capable of increasing salt tolerance in a cell, comprising a nucleic acid molecule selected from the group consisting of (a) a nucleic acid molecule that hybridizes to all or part of a nucleic molecule in [SEQ ID NO:5], [SEQ ID NO:7], [SEQ ID NO:9] or to a nucleic acid molecule comprising about nucleotides 1-1487 of [SEQ ID
NO:9], or a complement thereof under moderate or high stringency hybridization conditions, wherein the nucleic acid molecule encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell;
(b) a nucleic add molecule degenerate with respect to (a), wherein the nucleic molecule encodes a TNHX polypeptide, a PNHX polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
(c) the nucleic acid molecule of the coding strand shown in [SEQ ID
NO:5], [SEQ ID NO:7], [SEQ ID NO:9], nucleotides 1-1487 of [SEQ ID
NO:9], or an isolated nucleic acid molecule including about 1614 nucleic acids including [SEQ ID NO:5], [SEQ ID NO:7], nucleotides 1 to 1487 of the nucleic add molecule in [SEQ ID NO:9] or the complement thereof;
(d) a nucleic acid molecule encoding the same amino and sequence as a nucleotide sequence of (c); and (e) a nucleic acid molecule having of least 17% sequence identity with the nucleotide sequence of (e) and which encodes a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell.
54. A polypeptide produced from the nucleic acid molecule of claim 53.
55. A polypeptide comprising (a) the amino acid sequence in [SEQ ID NO:6], [SEQ
ID NO:8], [SEQ ID NO:10] (b) amino acids 1 to 496 of [SEQ ID NO:10]; and (c) a sequence having greater than 28% homology to the polypeptide in (a) or (b).
56. The polypeptide of claim 55, wherein the polypeptide comprises a Na+/H+
transporter polypeptide capable of increasing salt tolerance in a Cell.
57. A DNA molecule encoding the polypeptide of claim 55.
58. A method of producing a genetically transformed plant which expresses or overexpressss a TNHX transporter polypeptide, a PNHX transporter polypeptide or a polypeptide having Na+/H+ transporter activity and capable of increasing salt tolerance in a cell and wherein the plant has increased salt tolerance, comprising:
(a) cloning or synthesizing a TNHX nucleic acid molecule, a PNHX
nucleic acid molecule or a nucleic acid molecule which codes for a Na+/H+ transporter polypeptide, wherein the potypeptide is capable of providing salt tolerance to a plant;
(d) inserting the nucleic acid molecule in a vector so that the nucleic acid molecule is operably linked to a promoter;
(c) inserting the vector into a plant cell or plant seed;
(d) regenerating the plant from the plant cell or plant seed, wherein salt tolerance in the plant is increased compared to a wild type plant.
58. A transgenic plant produced according to the method of claim 57.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7847498P | 1998-03-18 | 1998-03-18 | |
| US60/078,474 | 1998-03-18 | ||
| US11611199P | 1999-01-15 | 1999-01-15 | |
| US60/116,111 | 1999-01-15 | ||
| PCT/CA1999/000219 WO1999047679A2 (en) | 1998-03-18 | 1999-03-18 | Genetic engineering salt tolerance in crop plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2323756A1 true CA2323756A1 (en) | 1999-09-23 |
| CA2323756C CA2323756C (en) | 2010-09-21 |
Family
ID=26760573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2323756A Expired - Lifetime CA2323756C (en) | 1998-03-18 | 1999-03-18 | Genetic engineering salt tolerance in crop plants |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU2821499A (en) |
| CA (1) | CA2323756C (en) |
| WO (1) | WO1999047679A2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009053511A1 (en) | 2007-10-24 | 2009-04-30 | Navarro Avino Juan Pedro | Method for improving salinity tolerance |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU782483B2 (en) * | 1999-11-10 | 2005-08-04 | Beth Israel Deaconess Medical Center | Stress-resistant oversized transgenic plants capable of growing in salinized soil |
| US7041875B1 (en) | 1998-03-18 | 2006-05-09 | Eduardo Blumwald | Genetic engineering salt tolerance in crop plants |
| US6936750B2 (en) | 1998-03-18 | 2005-08-30 | Eduardo Blumwald | Increasing salt tolerance in plants by overexpression of a vacuolar Na+/H+ transporter[s] |
| US7442852B2 (en) | 1998-03-18 | 2008-10-28 | Eduardo Blumwald | High salt plants and uses for bioremediation |
| JP3955938B2 (en) | 1998-12-22 | 2007-08-08 | 独立行政法人農業生物資源研究所 | Sodium / proton antiporter gene |
| US6803500B1 (en) * | 1999-08-24 | 2004-10-12 | Suntory Limited | Genes encoding proteins regulating the pH of vacuoles |
| US20020178464A1 (en) | 1999-11-10 | 2002-11-28 | Whitehead Institute For Biomedical Research | Proton transporters and uses in plants |
| US7534933B2 (en) | 2000-08-18 | 2009-05-19 | University Of Connecticut | Transgenic plants overexpressing a plant vacuolar H + -ATPase |
| US8697950B2 (en) | 1999-11-10 | 2014-04-15 | University Of Connecticut | Vacuolar pyrophosphatases and uses in plants |
| EP1315410A4 (en) * | 2000-08-18 | 2004-06-23 | Univ Connecticut | Enhanced meristematic activity and competence by overexpression of tonoplast pyrophosphatase |
| EP1339849B1 (en) | 2000-08-25 | 2006-10-18 | BASF Plant Science GmbH | PLANT POLYNUCLEOTIDES ENCODING NOVEL Na+ /H+ ANTIPORTERS |
| EP1970442B1 (en) | 2002-05-03 | 2017-01-18 | Monsanto Technology LLC | Transgenic high tryptophan plants |
| EP1539961B1 (en) * | 2002-07-12 | 2013-01-02 | The Regents of The University of California | Improved transporters and their uses |
| WO2009140206A1 (en) | 2008-05-11 | 2009-11-19 | The Gov. Of The U. S. A., As Represented By The Secretary Of The Dept. Of Health And Human Services | Improved expression of il-12 family heterodimers |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5272085A (en) * | 1989-10-31 | 1993-12-21 | Queen's University | Sodium tolerance genes derived from schizosaccharomyces pombe |
-
1999
- 1999-03-18 CA CA2323756A patent/CA2323756C/en not_active Expired - Lifetime
- 1999-03-18 AU AU28214/99A patent/AU2821499A/en not_active Abandoned
- 1999-03-18 WO PCT/CA1999/000219 patent/WO1999047679A2/en active Application Filing
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009053511A1 (en) | 2007-10-24 | 2009-04-30 | Navarro Avino Juan Pedro | Method for improving salinity tolerance |
Also Published As
| Publication number | Publication date |
|---|---|
| AU2821499A (en) | 1999-10-11 |
| CA2323756C (en) | 2010-09-21 |
| WO1999047679A3 (en) | 1999-11-25 |
| WO1999047679A2 (en) | 1999-09-23 |
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| Date | Code | Title | Description |
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| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20190318 |