CA2321215A1 - Hybrid adenovirus-aav virus and method of use thereof - Google Patents
Hybrid adenovirus-aav virus and method of use thereof Download PDFInfo
- Publication number
- CA2321215A1 CA2321215A1 CA002321215A CA2321215A CA2321215A1 CA 2321215 A1 CA2321215 A1 CA 2321215A1 CA 002321215 A CA002321215 A CA 002321215A CA 2321215 A CA2321215 A CA 2321215A CA 2321215 A1 CA2321215 A1 CA 2321215A1
- Authority
- CA
- Canada
- Prior art keywords
- hybrid
- aav
- transgene
- host cell
- aav virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The present invention provides a hybrid vector construct which comprises a portion of an adenovirus, 5" and 3" ITR sequences from an AAV, and a selected transgene. Also provided is a hybrid virus linked via a polycation conjugate to an AAV rep gene to form a single particle. These trans-infection particles are characterized by higher titer transgene delivery to a host cell and the ability to stably integrate the transgene into the host cell chromosome. Also disclosed is the use of the hybrid vectors and viruses to produce large quantities of recombinant AAV.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/331,384 US5856152A (en) | 1994-10-28 | 1994-10-28 | Hybrid adenovirus-AAV vector and methods of use therefor |
US08/331,384 | 1994-10-28 | ||
CA 2203808 CA2203808C (en) | 1994-10-28 | 1995-10-27 | Hybrid adenovirus-aav virus and method of use thereof |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2203808 Division CA2203808C (en) | 1994-10-28 | 1995-10-27 | Hybrid adenovirus-aav virus and method of use thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2321215A1 true CA2321215A1 (en) | 1996-05-09 |
CA2321215C CA2321215C (en) | 2009-04-14 |
Family
ID=25679278
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002321215A Expired - Lifetime CA2321215C (en) | 1994-10-28 | 1995-10-27 | Hybrid adenovirus-aav virus and method of use thereof |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2321215C (en) |
-
1995
- 1995-10-27 CA CA002321215A patent/CA2321215C/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2321215C (en) | 2009-04-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20151027 |