CA2319589A1 - Mechanical handling systems for laser capture microdissection - Google Patents

Mechanical handling systems for laser capture microdissection Download PDF

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Publication number
CA2319589A1
CA2319589A1 CA002319589A CA2319589A CA2319589A1 CA 2319589 A1 CA2319589 A1 CA 2319589A1 CA 002319589 A CA002319589 A CA 002319589A CA 2319589 A CA2319589 A CA 2319589A CA 2319589 A1 CA2319589 A1 CA 2319589A1
Authority
CA
Canada
Prior art keywords
transporting substrate
cellular material
tissue sample
mapped location
identified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002319589A
Other languages
French (fr)
Other versions
CA2319589C (en
Inventor
Seth R. Goldstein
Robert F. Bonner
Paul D. Smith
John Peterson
Thomas Pohida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
US Department of Health and Human Services
Original Assignee
The Government Of The United States Of America, Represented By The Secre Tary Of The Department Of Health And Human Services
Seth R. Goldstein
Robert F. Bonner
Paul D. Smith
John Peterson
Thomas Pohida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Government Of The United States Of America, Represented By The Secre Tary Of The Department Of Health And Human Services, Seth R. Goldstein, Robert F. Bonner, Paul D. Smith, John Peterson, Thomas Pohida filed Critical The Government Of The United States Of America, Represented By The Secre Tary Of The Department Of Health And Human Services
Publication of CA2319589A1 publication Critical patent/CA2319589A1/en
Application granted granted Critical
Publication of CA2319589C publication Critical patent/CA2319589C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00009—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with a sample supporting tape, e.g. with absorbent zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/282—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on with mapping; Identification of areas; Spatial correlated pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection

Landscapes

  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method and apparatus of gathering by LCM identified cellular material from random locations on a tissue sample to designated locations on a transporting substrate enables convenient further processing. A transporting substrate has an identified mapped location for receiving identified cellular material. At least a segment of a selectively activatable coating is placed on the side of the transporting substrate in apposition to the tissue sample at the mapped location. The transporting substrate and sample are relatively moved to place the selectively activated coating at the mapped location in apposition to identified cellular material of the tissue sample which is to be extracted. Thereafter, the selectively activatable coating is activated and impressed or impressed and activated to form an adhesive region on the transporting substrate for adhering to the identified cellular material. Upon removal of the transporting substrate from the tissue sample, identified cellular material adheres to the transporting substrate at the mapped location.
CA002319589A 1998-02-03 1999-01-29 Mechanical handling systems for laser capture microdissection Expired - Fee Related CA2319589C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7348098P 1998-02-03 1998-02-03
US60/073,480 1998-02-03
PCT/US1999/001987 WO1999039176A1 (en) 1998-02-03 1999-01-29 Mechanical handling systems for laser capture microdissection

Publications (2)

Publication Number Publication Date
CA2319589A1 true CA2319589A1 (en) 1999-08-05
CA2319589C CA2319589C (en) 2008-11-18

Family

ID=22113940

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002319589A Expired - Fee Related CA2319589C (en) 1998-02-03 1999-01-29 Mechanical handling systems for laser capture microdissection

Country Status (5)

Country Link
EP (1) EP1053461A1 (en)
JP (1) JP4344474B2 (en)
AU (1) AU749378B2 (en)
CA (1) CA2319589C (en)
WO (1) WO1999039176A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE449956T1 (en) * 1998-12-10 2009-12-15 Us Gov Health & Human Serv LASER ATTACHMENT MICRODISCISSION USING TWO LASER PULSES TO EXPAND AND RETRACT A LAYER
US6743601B1 (en) 1998-12-10 2004-06-01 The United States Of America As Represented By The Department Of Health And Human Services Non-contact laser capture microdissection
DE10015157A1 (en) 2000-03-27 2001-10-18 P A L M Gmbh Collecting device for objects dissolved out of mass, especially by laser radiation, has control system automatically generating accommodation unit displacement signals
TWI236500B (en) * 2002-10-18 2005-07-21 Ind Tech Res Inst Device and method for capturing biological tissues
DE10254229B4 (en) 2002-11-20 2004-10-28 P.A.L.M. Microlaser Technologies Ag Positioning device for positioning a collecting device of a laser microdissection system
DE102005007793B4 (en) * 2005-02-14 2013-03-07 Lim Laserinstitut Mittelsachsen Gmbh Method for both working out and releasing free-form bodies with laser beams of at least one laser from material blocks transparent to the laser wavelength used
DE102006051460A1 (en) * 2006-10-31 2008-05-08 P.A.L.M. Microlaser Technologies Gmbh Apparatus, methods and tape material for collecting and transporting sample material
DE102006051313A1 (en) * 2006-10-31 2008-05-08 Robert Bosch Gmbh Method for detecting impurities on a surface
WO2009105810A1 (en) * 2008-02-25 2009-09-03 Bizpac (Australia) Pty Ltd Punch sampling apparatus and method
AU2011220513B2 (en) 2010-02-25 2014-07-31 Prairie Ventures, L.L.C. System and method for anatomical pathology sample handling, storage, and analysis
JP6611610B2 (en) * 2013-10-07 2019-11-27 国立大学法人名古屋大学 Laser microdissection device, analyzer including the laser microdissection device, and method for manufacturing microchip
AU2018317826B2 (en) * 2017-08-15 2022-11-24 Pacific Biosciences Of California, Inc. Scanning apparatus and methods useful for detection of chemical and biological analytes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843657A (en) 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US6100051A (en) * 1997-06-27 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Method utilizing convex geometry for laser capture microdissection

Also Published As

Publication number Publication date
EP1053461A1 (en) 2000-11-22
JP2002502025A (en) 2002-01-22
AU2485099A (en) 1999-08-16
WO1999039176A1 (en) 1999-08-05
CA2319589C (en) 2008-11-18
JP4344474B2 (en) 2009-10-14
AU749378B2 (en) 2002-06-27

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Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20140129