CA2318737A1 - Procedes de fabrication de vecteurs pseudo-adenoviraux - Google Patents

Procedes de fabrication de vecteurs pseudo-adenoviraux Download PDF

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Publication number
CA2318737A1
CA2318737A1 CA002318737A CA2318737A CA2318737A1 CA 2318737 A1 CA2318737 A1 CA 2318737A1 CA 002318737 A CA002318737 A CA 002318737A CA 2318737 A CA2318737 A CA 2318737A CA 2318737 A1 CA2318737 A1 CA 2318737A1
Authority
CA
Canada
Prior art keywords
adenovirus
genome
helper
packaging signal
packaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002318737A
Other languages
English (en)
Inventor
Samuel C. Wadsworth
Helen Romanczuk
Richard J. Gregory
Donna Armentano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genzyme Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2318737A1 publication Critical patent/CA2318737A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des adénovirus auxiliaires qui facilitent la production de vecteurs pseudo-adénoviraux (PAV) mais qui ne peuvent pas être encapsidés dans des particules virales. L'invention concerne également de nouvelles lignées cellulaires de production de PAV qui expriment les protéines liantes ou les répresseurs des ADN empêchant l'encapsidation des adénovirus auxiliaires. En outre, l'invention concerne des procédés de fabrication de PAV dans ces lignées cellulaires avec un minimum de contamination due aux virus auxiliaires.
CA002318737A 1998-02-17 1999-02-17 Procedes de fabrication de vecteurs pseudo-adenoviraux Abandoned CA2318737A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US7476198P 1998-02-17 1998-02-17
US60/074,761 1998-02-17
US8652898P 1998-05-22 1998-05-22
US60/086,528 1998-05-22
PCT/US1999/003483 WO1999041400A1 (fr) 1998-02-17 1999-02-17 Procedes de fabrication de vecteurs pseudo-adenoviraux

Publications (1)

Publication Number Publication Date
CA2318737A1 true CA2318737A1 (fr) 1999-08-19

Family

ID=26756027

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002318737A Abandoned CA2318737A1 (fr) 1998-02-17 1999-02-17 Procedes de fabrication de vecteurs pseudo-adenoviraux

Country Status (5)

Country Link
EP (1) EP1054989A1 (fr)
JP (1) JP2002538758A (fr)
AU (1) AU752284B2 (fr)
CA (1) CA2318737A1 (fr)
WO (1) WO1999041400A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020136708A1 (en) 1993-06-24 2002-09-26 Graham Frank L. System for production of helper dependent adenovirus vectors based on use of endonucleases
US7045347B2 (en) 1993-06-24 2006-05-16 Advec, Inc. Helper dependent adenovirus vectors based on integrase family site-specific recombinases
US20020146392A1 (en) * 1993-06-24 2002-10-10 Frank L. Graham Helper dependent adenovirus vectors based on site-specific recombinases
AU2004201152B2 (en) * 1995-06-07 2007-03-08 Advec, Inc. Helper Dependent Adenovirus Vectors Based on Site-specific Recombinases
AU7579100A (en) * 1999-09-23 2001-04-24 Genzyme Corporation Helper vectors and cell lines for the production of pseudoadenoviral vectors
US6867022B1 (en) 2000-01-21 2005-03-15 Regents Of The University Of Michigan Replication deficient adenovirus vectors and methods of making and using them
WO2001097829A2 (fr) 2000-06-19 2001-12-27 Genzyme Corporation Traitement de substitution enzymatique, therapie genique et therapie par petites molecules pour maladies liees au stockage lysosomal
US20020081707A1 (en) * 2000-06-29 2002-06-27 Donna Armentano Methods for helper-dependent adenoviral vector production
WO2003008599A2 (fr) * 2001-07-18 2003-01-30 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung Systeme destine a produire des populations clonales ou complexes d'adenovirus recombines et leur application
EP1809753A1 (fr) * 2004-11-02 2007-07-25 Istituto di Richerche di Biologia Molecolare P. Angeletti S.p.A. Amplicon adenoviral et cellules productrices de vecteurs adenoviraux a defaut de replication, et leurs methodes de preparation et d'utilisation
SI2889043T1 (sl) 2008-12-16 2019-08-30 Genzyme Corporation Sintetične vmesne spojine za pripravo konjugatov oligosaharid-protein

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2737501B1 (fr) * 1995-07-31 1997-10-24 Transgene Sa Nouveaux virus auxiliaires pour la preparation de vecteurs viraux recombinants
WO1997032481A1 (fr) * 1996-03-07 1997-09-12 The Regents Of The University Of California Adenovirus sans assistant et totalement defectif pour therapie genetique

Also Published As

Publication number Publication date
JP2002538758A (ja) 2002-11-19
EP1054989A1 (fr) 2000-11-29
AU752284B2 (en) 2002-09-12
WO1999041400A1 (fr) 1999-08-19
AU2771899A (en) 1999-08-30

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Legal Events

Date Code Title Description
FZDE Discontinued