CA2311571A1 - Isolation et caracterisation de sequences regulatrices de plantes - Google Patents

Isolation et caracterisation de sequences regulatrices de plantes Download PDF

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Publication number
CA2311571A1
CA2311571A1 CA002311571A CA2311571A CA2311571A1 CA 2311571 A1 CA2311571 A1 CA 2311571A1 CA 002311571 A CA002311571 A CA 002311571A CA 2311571 A CA2311571 A CA 2311571A CA 2311571 A1 CA2311571 A1 CA 2311571A1
Authority
CA
Canada
Prior art keywords
plant
nematode
dna
gus
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002311571A
Other languages
English (en)
Inventor
Nathalie Barthels
Mansour Karimi Dehkor Di
Godelieve Gheysen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2311571A1 publication Critical patent/CA2311571A1/fr
Abandoned legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Communicable Diseases (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Dans la recherche de séquences régulatrices de plantes capables d'entraîner l'expression du gène effecteur déclenchée par les nématodes dans les structures d'alimentation, le marquage par promoteurs semble être un outil précieux. Un grand nombre de plantes transgéniques Arabidopsis thaliana (L.) a été généré. Elles ont été transformées par un gène de .beta.-glucuronidase fonctionnant comme marqueur de promoteur. Deux produits de reconstruction d'ADN-T, pGV1047 et p.DELTA.gusBin19 ont été utilisés. Les réponses précoces à l'invasion par les nématodes ont été trés interessantes. Quatre lignées présentant une activité GUS dans les syncytia induite par les nématodes à kystes de la betterave ont été étudiées. L'activation du gène reporter a également été identifiée dans les galles induites par les nématodes ectoparasites et des neudosités des racines. Des études ont montré que les quatre marqueurs étaient activés de manière différentielle pendant le développement de la structure d'alimentation. Des zones adjacentes ADN-T responsables des réponses observées après la nématodose ont été isolées et caractérisées pour l'activité du promoteur.
CA002311571A 1997-11-27 1998-11-26 Isolation et caracterisation de sequences regulatrices de plantes Abandoned CA2311571A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97203701.4 1997-11-27
EP97203701 1997-11-27
PCT/EP1998/007761 WO1999028483A2 (fr) 1997-11-27 1998-11-26 Isolation et caracterisation de sequences regulatrices de plantes

Publications (1)

Publication Number Publication Date
CA2311571A1 true CA2311571A1 (fr) 1999-06-10

Family

ID=8228978

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002311571A Abandoned CA2311571A1 (fr) 1997-11-27 1998-11-26 Isolation et caracterisation de sequences regulatrices de plantes

Country Status (4)

Country Link
EP (1) EP1034285A2 (fr)
AU (1) AU748761B2 (fr)
CA (1) CA2311571A1 (fr)
WO (1) WO1999028483A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19960843A1 (de) * 1999-12-16 2001-06-28 Florian Grundler Wurzelspezifischer Promotor
FR2864104B1 (fr) * 2003-12-23 2006-02-17 Genoplante Valor Sequences nucleotidiques promotrices inductibles par infection par les pathogenes.
CA2603944C (fr) * 2005-04-08 2015-06-23 Bayer Bioscience N.V. Evenement elite a2704-12 comprenant l'integration du gene phosphinothri cine acetyltransferase dans le soya et procedes et trousses permettant d'identifier cet evenement dans des prelevements biologiques
BRPI0618373A2 (pt) 2005-11-08 2011-08-30 Pioneer Hi Bred Int molécula de ácido nucléico isolada, construção de dna, método para produção de uma planta, métodos para a expressão de uma seqüência de nucleotìdeos em uma célula de planta e em uma planta, método para expressão seletiva de uma seqüência de nucleotìdeos em uma raiz de planta
BRPI0708206A2 (pt) 2006-02-23 2011-05-17 Basf Plant Science Gmbh promotor, cassete de expressão, vetor, célula vegetal, planta transgênica, semente de planta, métodos para controlar uma infestação de nematóide parasìtico em plantas, e para conferir ou melhorar a reistência ao nematóide em um planta, e, uso de um promotor ou de um cassete de expressão ou de um vetor
US20100095404A1 (en) * 2007-03-15 2010-04-15 Basf Plant Science Gmbh Use of Nematode Chitinase Genes to Control Plant Parasitic Nematodes
CN104313035A (zh) * 2014-10-17 2015-01-28 扬州大学 茄子抗根结线虫相关基因及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2143000C1 (ru) * 1991-11-20 1999-12-20 Моген Интернэшнл Н.В. Способ получения растения с пониженной восприимчивостью к растительным паразитическим нематодам (варианты), рекомбинантная днк (варианты), трансформирующий растение вектор, штамм agrobacterium и способ снижения ущерба урожаю
WO1994010320A1 (fr) * 1992-11-02 1994-05-11 Mogen International N.V. Plantes presentant une susceptibilite reduite a des attaques de parasites tels que des nematodes
US5612471A (en) * 1994-05-25 1997-03-18 The Regents Of The University Of California Nematode-induced genes in tomato

Also Published As

Publication number Publication date
AU2513199A (en) 1999-06-16
AU748761B2 (en) 2002-06-13
WO1999028483A2 (fr) 1999-06-10
EP1034285A2 (fr) 2000-09-13
WO1999028483A3 (fr) 1999-07-22

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EEER Examination request
FZDE Discontinued
FZDE Discontinued

Effective date: 20051128