CA2306565A1 - Carbapenem antibacterial compounds, compositions and methods of treatment - Google Patents

Abstract

Compounds of formula (I) are disclosed as well as pharmaceutically acceptable salts thereof. The naphthosultam is substituted with various substituent groups including at least one cationic group -A-Q-L-B. The carbapenems of the invention are effective against susceptible bacterial organisms, including methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant Staphylococcus epidermidis (MRSE), and methicillin resistant coagulase negative Staphylococci (MRCNS).

Description

WO 99/20627 -x- PCT/US98/22110 TITLE OF THE INVENTION
CARBAPENEM ANTIBACTERIAL COMPOUNDS, COMPOSITIONS AND METHODS OF TREATMENT
BACKGROUND OF THE INVENTION
The present invention relates to carbapenem antibacterial agents in which the carbapenem nucleus is substituted at the 2-position with a naphthosultam linked through a CH2 group.
The naphthosultam is further substituted with various substituent groups including at least one cationic group -A-(a-L-B.
The carbapenems of the present invention are useful against gram positive microorganisms, especially methicillin resistant Staphylococcus aureus (MRSA), methicillin resistant Staphylococcus epidermidis (MRSE), and methicillin resistant 20 coagulase negative Staphylococci (MRCNS), and are also active against Gram negative bacteria. There is an increasing need for agents effective against such pathogens (MRSA/MRCNS) which are at the same time relatively free from undesirable side effects. The antibacterial compounds of the present invention thus comprise an important contribution to therapy for treating infections caused by these difficult to control pathogens.
SUMMARY OF THE INVENTION
The present invention relates to a compound represented by formula I:
tR)2 P H H R
H3C ~ CH2-N \
/~- N ~ i C02M ~=S \ J
O A-Q-L-B

WO 99/20627 _2_ PCT/US98/22110 as well as pharmaceutically acceptable salts thereof, wherein:
R1 represents H or methyl;
C02M represents a carboxylic acid, a carboxylate anion, a pharmaceutically acceptable ester group or a carboxylic acid protected by a protecting group;
P represents hydrogen, hydroxyl, F or hydroxyl protected by a hydroxyl-protecting group;
A-Q-L-B represents a side chain wherein:
A is a C 1-6 alkylene group, straight or branched, and 10 optionally interrupted or terminated by 1-2 of -O-, -S-, NRa-, -C(O)-and -CH=CH-;
fa represents R ~ ~ /Rb ~N~ H ~~~

2)b X
in which:
bis2or3;
and X- is a charge balancing counterion;
L represents a C1-g alkylene group, unsubstituted or substituted with 1-3 Rc groups, and is interrupted or terminated by 1-3 of -CH=CH-, -C(O)- , -C(O)NRd-, -Het(Re)- , -C(O)-Het(Re)- , _C(O)NR,a_get(Re)- , -O- , -S- , -S(O)-, -S02-, -C02- , -NRa- , -N+(Ra)z_ -C O ~ ~ -C O N Ra ~I ~I ~ ) ~I
Re . Re and Re Het is a heteroaryl group;
B represents -~NRfR9Rh or -NRfR9 or WO 99/20627 _$- PCT/US98/22110 ~Re~2 (Re)2 I \~ ~ I
N -~- R b )o-1 N
2) or N
wherein represents a 5-10 membered mono- or bicyclic, N-containing heteroaryl group, optionally containing 1-4 additional heteroatoms selected from O, S and N;
Ra is H or C1_g alkyl;
Rb is NH2 or C1_6 alkyl unsubstituted or substituted with 1-3 groups selected from halo, OH, CN and C(O)NH2;
Rc is independently selected from halo, ORa, SRa, OC(O)Ra, C02Ra, CN, C(O)N(Ra)2 and C(O)Ra, Rd is H or C1_g alkyl, or Rc and Rd taken together with any intervening atoms represent a 4-6 membered ring;
Re is H; Rc; N02 ; N(Ra)2 ; S02N(Ra)2 or C1_4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH
and C(O)NH2;
15 Rf Rg and Rh are independently selected from H; C1-6 straight or branched chain alkyl, unsubstituted or substituted with 1-3 Rc groups; C3_g cycloalkyl, unsubstituted or substituted with 1-3 Rc groups; phenyl, unsubstituted or substituted with 1-3 Re groups and Het, unsubstituted or substituted with 1-3 Re groups, or Rf and Rg taken together with the intervening N atom form a 4-6 membered ring, optionally interrupted by 1-2 of O, S, C(O) or NRh, and optionally substituted by 1-3 Rc groups;
and each R independently represents H; N02 ; N(Ra)2;
25 S02N(Ra)~ ; Rc or C1_4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH, C(O)NH2~
or R together with A of the group -A-Q-L-B and any intervening atoms represent a 5-6 membered carbocyclic ring.

WO 99/20627 ~_ PCT/US98/22110 Pharmaceutical compositions and methods of treatment are also included.
DETAILED DESCRIPTION OF THE INVENTION
The invention is described herein in detail using the terms defined below unless otherwise specified.
Carboxylate anion refers to a negatively charged group -COO-.
The term "alkyl" refers to a monovalent alkane 10 (hydrocarbon) derived radical containing from 1 to 15 carbon atoms unless otherwise defined. It may be straight, branched or cyclic.
Preferred alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, t-butyl, cyclopentyl and cyclohexyl. When substituted, alkyl groups may be substituted with up to four substituent groups, selected from 15 Rc, Rf and Rg, or as otherwise defined, at any available point of attachment. When the alkyl group is said to be substituted with an alkyl group, this is used interchangeably with "branched alkyl group". When a bond appears without a group attached, this signifies the presence of a methyl group.
20 Cycloalkyl is a specie of alkyl containing from 3 to carbon atoms, without alternating or resonating double bonds between carbon atoms. It may contain from 1 to 4 rings which are fused.
The term "alkenyl" refers to a hydrocarbon radical 25 straight, branched or cyclic containing from 2 to 10 carbon atoms and at least one carbon to carbon double bond. Preferred alkenyl groups include ethenyl, propenyl, butenyl and cyclohexenyl.
The term "alkynyl" refers to a hydrocarbon radical straight or branched, containing from 2 to 10 carbon atoms and at 30 least one carbon to carbon triple bond. Preferred alkynyl groups include ethynyl, propynyl and butynyl.
Aryl refers to aromatic rings e.g., phenyl, substituted phenyl and the like, as well as rings which are fused, e.g., naphthyl, phenanthrenyl and the like. An aryl group thus contains at least one 35 ring having at least 6 atoms, with up to five such rings being present, containing up to 22 atoms therein, with alternating (resonating) double bonds between adjacent carbon atoms or suitable heteroatoms.
The preferred aryl groups are phenyl, naphthyl and phenanthrenyl.
Aryl groups may likewise be substituted as defined. Preferred substituted aryls include phenyl and naphthyl.
5 The term "heteroaryl" (Het) refers to a monocyclic aromatic hydrocarbon group having 5 or 6 ring atoms, or a bicyclic aromatic group having 8 to 10 atoms, containing at least one heteroatom, O, S or N, and in which 1-2 additional carbon atoms are optionally replaced by a heteroatom selected from O or S, and in which from 1-3 additional carbon atoms are optionally replaced by N, said heteroaryl group being optionally substituted as described herein. Examples include the following:
CNH ~NH ~S
pyrroie (pyrrolyl) imidazole (imidazoly!) thiazole (thiazolyl) N ~O CO CS
oxazole (oxazolyl) furan (furyi) thiophene (thienyl) N~NH .~ ,NH ~ ,O
N N
triazole (triazolyl) pyrazole (pyrazolyl) isoxazoie (isoxazolyl) N
~s I~ I
N N N
isothiazole (isothiazolyl) pyridine (pyridinyl) pyrazine (pyrazinyl) ~~ N
I :N I NJ
N
pyridazine (pyridazinyl) pyrimidine (pyrimidinyl) N~N
~NJ
triazine (triazinyi) Heteroaryl includes protonated forms as well. It thus includes positively charged as well as non-charged groups.
Examples include the groups shown above, having an additional H
attached to a nitrogen atom.
The group A-Q-L-B is attached to one of the two phenyl rings shown.
Heteroarylium refers to heteroaryl groups bearing a quaternary yr protonated atom and thus a positive charge.
Examples include the following:

~N~/N-CH3 ~-N~/S
~~ N + +
.~N~~ ~ ,N CH3 ~ ,O
+ i + ;+
+ N
~N~ i \
~ \ N-CH3 N~/ + N N +

~N/ ~ ~N
,S ~ J + ~L J
i+ N N
\ NnN.CHs ~N.N ~N~
,+

When a charge is shown on a particular nitrogen atom in a ring or in a nitrogen containing non-ring moiety which contains 5 one or more additional nitrogen atoms, it is understood that the charge may reside on a different nitrogen atom than that shown in a particular drawing by virtue of charge resonance that occurs.
~N~ ~N~ +
NON-CH3 ~ N~N_.CHs 10 and ~N~N-CHs ...--~ ~N ~ CH3 + iNw/
Likewise, when a basic nitrogen containing moiety is provided at an appropriately acidic pH, the moiety becomes wo ~no62~ -$- pcTn~s9sn2mo protonated due to acid base reactivity. Both the protonated and non-protonated forms of the compounds of formula I are included in the present invention.
The term "heterocycloalkyl" refers to a cycloalkyl group 5 (nonaromatic) in which one of the carbon atoms in the ring is replaced by a heteroatom selected from O, S or N, and in which up to three additional carbon atoms may be replaced by hetero atoms.
The terms "quaternary nitrogen" and "positive charge"
refer to tetravalent, positively charged atoms including, e.g., the 10 positively charged nitrogen in a tetraalkylammonium group (e. g.
tetramethylammonium), heteroarylium, (e.g., N-methyl-pyridinium), basic nitrogens which are protonated at physiological pH, and the like. Cationic groups thus encompass positively charged nitrogen-containing groups, as well as basic nitrogens which are 15 protonated at physiologic pH.
The term "heteroatom" means O, S or N, selected on an independent basis.
Halogen and "halo" refer to bromine, chlorine, fluorine and iodine.
20 Alkoxy refers to C1-C4 alkyl-O-, with the alkyl group optionally substituted as described herein.
When a group is termed "substituted", unless otherwise indicated, this means that the group contains from 1 to 4 substituents thereon.
25 When a functional group is termed "protected", this means that the group is in modified form to preclude undesired side reactions at the protected site. Suitable protecting groups for the compounds of the present invention will be recognized from the present application taking into account the level of skill in the art, 30 and with reference to standard textbooks, such as Greene, T. W. et al.
Protective Groups in Organic Synthesis Wiley, New York (1991).
Examples of suitable protecting groups are contained throughout the specification.
In some of the carbapenem compounds of the present 35 invention, M is a readily removable carboxyl protecting group, and/or P represents a hydroxyl which is protected by a hydroxyl-protecting WO 99/20627 _g_ PC"T/US98/22110 group. Such conventional protecting groups consist of groups which are used to protectively block the hydroxyl or carboxyl group during the synthesis procedures described herein. These conventional blocking groups are readily removable, i.e., they can be removed, if 5 desired, by procedures which will not cause cleavage or other disruption of the remaining portions of the molecule. Such procedures include chemical and enzymatic hydrolysis, treatment with chemical reducing or oxidizing agents under mild conditions, treatment with a transition metal catalyst and a nucleophile and 10 catalytic hydrogenation.
Examples of carboxyl protecting groups include allyl, benzhydryl, 2-naphthylmethyl, benzyl, silyl such as t-butyldimethylsilyl (TBDMS), phenacyl, p-methoxybenzyl, o-nitrobenzyl, p-methoxyphenyl, p-nitrobenzyl, 4-pyridylmethyl 15 and t-butyl.
Examples of suitable hydroxyl protecting groups include triethylsilyl, t-butyldimethylsilyl, o-nitrobenzyloxycarbonyl, p-nitrobenzyloxycarbonyl, benzyloxycarbonyl, allyloxycarbonyl, t-butyloxycarbonyl, 2,2,2-trichloroethyloxycarbonyl and the like.
20 The carbapenem compounds of the present invention are useful per se and in their pharmaceutically acceptable salt and ester forms for the treatment of bacterial infections in animal and human subjects. The term "pharmaceutically acceptable ester, salt or hydrate," refers to those salts, esters and hydrated forms of the 25 compounds of the present invention which would be apparent to the pharmaceutical chemist. i.e., those which are substantially non-toxic and which may favorably affect the pharmacokinetic properties of said compounds, such as palatability, absorption, distribution, metabolism and excretion. Other factors, more practical in nature, 30 which are also important in the selection, are cost of the raw materials, ease of crystallization, yield, stability, solubility, hygroscopicity and flowability of the resulting bulk drug.
Conveniently, pharmaceutical compositions may be prepared from the active ingredients in combination with pharmaceutically 35 acceptable carriers. Thus, the present invention is also concerned with pharmaceutical compositions and methods of treating bacterial WO 99/20627 -1~_ PCT/US98/22110 infections utilizing as an active ingredient the novel carbapenem compounds.
With respect to -C02M, which is attached to the carbapenem nucleus at position 3, this represents a carboxylic 5 acid group (M represents H), a carboxylate anion (M represents a negative charge), a pharmaceutically acceptable ester (M
represents an ester forming group) or a carboxylic acid protected by a protecting group (M represents a carboxyl protecting group). The pharmaceutically acceptable salts referred to above may take the 10 form -LOOM, where M is a negative charge, which is balanced by a counterion, e.g., an alkali metal cation such as sodium or potassium.
Other pharmaceutically acceptable counterions may be calcium, magnesium, zinc, ammonium, or alkylammonium cations such as tetramethylammonium, tetrabutylammonium, choline, 15 triethylhydroammonium, meglumine, triethanolhydroammonium, etc.
The pharmaceutically acceptable salts referred to above also include acid addition salts. Thus, the Formula I compounds can be used in the form of salts derived from inorganic or organic acids.
20 Included among such salts are the following: acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, 25 hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, tosylate and undecanoate.
30 The pharmaceutically acceptable esters are such as would be readily apparent to a medicinal chemist, and include, for example, those described in detail in U.S. Pat. No. 4,309,438.
Included within such pharmaceutically acceptable esters are those which are hydrolyzed under physiological conditions, such as 35 pivaloyloxymethyl, acetoxymethyl, phthalidyl, indanyl and WO 99/20627 _11- PGT/US98/22110 methoxymethyl, and others described in detail in U.S. Pat. No.

4,479,947. These are also referred to as "biolabile esters".
Acid addition salts of the compounds of formula I are likely protonated at physiological pH, as mentioned above.

5 Compounds such as those containing a basic N-containing moiety are capable of protonation in water at or near pH 7, so that the moiety can exist in its neutral form or as an acid addition (protonated) form.
X- is a charge balancing group.
Biolabile esters are biologically hydrolizable, and may be suitable for oral administration, due to good absorption through the stomach or intenstinal mucosa, resistance to gastric acid degradation and other factors. Examples of biolabile esters include compounds in which M represents an alkoxyalkyl, alkylcarbonyloxyalkyl, alkoxycarbonyloxyalkyl, cycloalkoxyalkyl, 15 alkenyloxyalkyl, aryloxyalkyl, alkoxyaryl, alkylthioalkyl, cycloalkylthioalkyl, alkenylthioalkyl, arylthioalkyl or alkylthioaryl group. These groups can be substituted in the alkyl or aryl portions thereof with acyl or halo groups. The following M species are examples of biolabile ester forming moieties: acetoxymethyl, 1-20 acetoxyethyl, 1-acetoxypropyl, pivaloyloxymethyl, 1-isopropyloxycarbonyloxyethyl, 1-cyclohexyloxycarbonyloxyethyl, phthalidyl and (2-oxo-5-methyl-1,3-dioxolen-4-yl)methyl.
For the purposes of this invention, all compounds which have one or more cations are balanced with one or more, as 25 necessary, of a charge balancing group X-. Examples of cases where a charge balancing group is required are quarternized substituents such as heteroarylium or C(=N+RHR')RA, where Ra and Rf are not H.
Additionally, all compounds having one or more anions are counter balanced with one or more, as necessary, charge balancing cations.
30 When a group is interrupted by 2-3 of O, S, or N they cannot form O-O, O-O-O, O-S, O-S-O, S-S, or S-S-S bonds. This is exemplified in the case when L or A is an alkylene interrupted or terminated by 1-3 of O, S, -S(O)-, -SOZ-, NRa, COZ C(O)NR~,...and the like.

WO 99/20627 _12_ PCT/US98/22110 X' can be present or absent as necessary to maintain the appropriate charge balance. When present, these represent pharmaceutically acceptable counterions. Most anions derived from inorganic or organic acids are suitable. Representative examples of 5 such counterions are the following: acetate, adipate, aminosalicylate, anhydromethylenecitrate, ascorbate, aspartate, benzoate, benzenesulfonate, bromide, citrate, camphorate, camphorsulfonate, chloride, estolate, ethanesulfonate, fumarate, glucoheptanoate, gluconate, glutamate, lactobionate, malate, 10 maleate, mandelate, methanesulfonate, pantothenate, pectinate, phosphate/diphosphate, polygalacturonate, propionate, salicylate, stearate, succinate, sulfate, tartrate and tosylate. Other suitable anionic species will be apparent to the ordinarily skilled chemist.
Likewise, when more than one negative charge is 15 necessary to maintain charge neutrality, the counterion indicator X' represents a specie with more than one negative charge, such as malonate, tartrate or ethylenediaminetetraacetate (EDTA), or when a multivalent negatively charged counterion is present with a carbapenem which bears a net single positive charge, an appropriate 20 number of carbapenem molecules can be found in association therewith to maintain the overall charge balance and neutrality.
Numbering and nomenclature using in naming the naphthosultams are as follows:

WO 99/20627 _13- PCT/(TS98/Z2110 Naphthosultam Nomenclature Chemical Abstracts 6 / \ 3 ~/ 7 \ ~ / 2 HN -O~ O '~~N O, O
1,8-naphthosultam 1,8-naphthosultamyl \ 4 / ~ \ 7 \ / 3 \ / 8 H N -S,' ~N -S,1 z ~ O '~2 ~ O
2H naphth[1,8-cdJisothiazole, 1,1-dioxo-2H naphth[1,8-cdJisothiazol-2-yl 1, i -dioxide I~JPAC (from Beilstein's Autonomy 4 / \ 7 / 3 \ ~ / 8 l HN-S: ~N-S:
z ~' O ''~,~ z ~' O
2 H~1-thia-2-aza-acenaphthalene, 1,1-dioxo-2H-1-thia-2-aza-acenaphthalen-2-yl 1,1-dioxide The -A- portion of the side chain is a C1_6 alkylene group 5 which is straight or branched, and is optionally interrupted or terminated by 1-2 of O, S, NRa, C(O), and -CH=CH-. The interrupting groups can be separate or together, and can terminate the C1_6 alkylene group. Also, the interrupting or terminating moiety can be between the alkylene group and the naphthosultam or -Q-. For 10 example, A can represent -p_C 1-6 alkyl- , -C 1_g alkyl -O- , 7 -14, PCTNS98/22110 -NRa-1-6 alkyl- and the like.
~,l represents ~Rb ~N~ H ~O~
2)b X
in which:
bis2or3;
and X- is a charge balancing counterion.
L represents a C1_g alkylene group, unsubstituted or substituted with 1-3 Rc groups, and is interrupted or terminated by 1-3 of -CH=CH-, -C(O)- , -C(O)NRd-, -Het(Re)- , -C(O)-Het(Re)- , -C(O)NRa- , -Het(Re)- , -O- , -S- , -S(O)-, -S02-, -C02- , -NRa- , -N+(Ra)2_ -CO ~ ~ -CONRa ) Re , Re and Re .
These interrupting or terminating moieties can be separate or 15 together. As described above with respect to A, the moieties can be at the ends of the C1_g alkylene group, and between the C1_g alkylene moiety and Q or B.
B represents ~RfRgRh or -NRfR9 or ~Re)2 ~Re)2 I \~+ ~ I
N -~-Rb)o-~ N
or N
wherein represents a 5-10 membered mono- or bicyclic, N-containing heteroaryl group, optionally containing 1-4 additional heteroatoms selected from O, S and N. These include positively charged as well as neutral moieties, many of which become positively charged at neutral to acidic pH. All are included in the present invention.
5 When Rb represents C1_g alkyl, it may be unsubstituted or substituted with 1-3 groups selected from halo, OH, CN and C(O)NH2.
When Rc and Rd both appear, they may be taken in combination with any intervening atoms to farm a 4-6 membered i 0 ring.
When Rf and Rg are both present, they can be taken together with the intervening atoms to form a 4-6 membered ring, which is optionally interrupted by 1-2 of O, S, C(O) and NRh. The ring can be unsubstituted or substituted with 1-3 Rc groups.
15 When one of the R groups is taken in combination with A
from the side chain A-Q-L-B, along with the intervening atoms, it represents a 5-6 membered carbocyclic ring.
A subset of compounds of formula I which is of interest relates to those compounds where Rl represents methyl. Within this 20 subset, all other variables are as originally defined.
Another subset of compounds of formula I which is of interest relates to those compounds where COZM represents a carboxylate anion. Hence, M in this instance represents a negative charge which is balanced by a positively charged group, such as in 25 the positively charged Q group.
Another subset of compounds of formula I that is of interest relates to those compounds where P represents hydroxyl or hydroxyl protected by a hydroxyl protecting group. Within this subset, all other variables are as originally defined.
30 Another subset of compounds of formula I that is of interest relates to compounds of formula I wherein A represents C1-3 alkylene. Within this subset, all other variables are as originally defined.
Another subset of compounds of formula I that is of 35 interest relates to compounds where L is C 1-5 alkylene that is interrupted or terminated by -C(O)NRd-, -C(O)NRa-Het(Re}-, -NRa-WO 99/20627 -1~ PCT/US98/22110 -C(O) N Ra , -N+(Ra)2 or RQ . Within this subset, all other variables are as originally defined.
Another subset of compounds of formula I that is of interest relates to compounds where B represents O
1) -NRiRsRh or -NRfR9.
Within this subset, all other variables. are as originally defined.
A preferred subset of compounds of formula I which is of interest relates to those compounds wherein:
10 Rl represents methyl;
C02M represents a carboxylate anion;
P represents hydroxyl or hydroxyl protected by a hydroxyl protecting group;
A represents C1_3 alkylene;
15 Q represents R~ ~ ~Rb N O+
OU
X-in which:
bis2or3, and X- is a charge balancing counterion;
20 L is C 1-5 alkylene, interrupted or terminated by -C(O)NRd-, -C(O)NRa-Het(Re)-, -NRa- , -N+(Ra)2 - or -C(O)NRa Re and B represents: -ORfR9Rh or -N RfR9.
All other variables are as originally defined.
25 Representative examples of compounds of the invention are found in Tables I - III.

wo ~no62~ -17- Pcr~rs9gnzmo Table I
Q-L-B
OH CH3 \
H H
'S
~2 O ~ 2-3 X

wherein Q-L-B is selected from:
\ /'~ \ 1 \ n ~--N N+ NH3+
~N+ N+~N+~ ~N ~N+
~/ \ \
\ ~ N~ ,~~, NH3+
\ S N+~~N+ ~.: N+~
\ /~'-\ N N~ \ ~----1 N ~ IV
N+ N+ ~ \- N+w ~ N+ N+ --~ ~= N+~
\ ~ ~- ~ 1 \ ~-\ 1 ~. N V + \, N+~ ~ N+ N+ ~ N- N+~
\ ~/ \
\ !~ ~"-N ~ N
~N+ N+ ~=N+~'NH3+ ~ \ N+-~/ \ ~/ U \

WO 99/20627 _lg_ PCT/US98/22110 N [~ N\_-.. N+~/NH3+
\ ~---1 \ N+~ \
- N+ +~
~N ~+ ~i \
NH3+
N I N /~
_ ~ \~N+~NH3+ ~ _ ~ ~-N+~
N ~/ + ~ N ~/ \
N
N, ~ N/~N
N N+ ~ ~ N+~ ~ ~ ~ ~ N+~
N ~/
~N ~+
~1 \ ~'\ ~N . N+~
~- N+ N+
~N
\ ~ ~N
\_-. N+~NH3+
N+ N+
NH3+
N N+~ N \

WO 99/20627 _19_ PCT/US98/22110 NH3+ - N+
\ n J--~N ~ ~ \ N
N+~
S N N+~ ~ Nj '-~ ~ 'N
n N~-N+
N+
v ~ ~ N
N J
N+~~N ~ ~ ~ 'N+
~N~ \
-N+
% U
/-\ . /
N+
~-\ N~ -N+ N+~ \
\ n ~ +- S N N+-' N !iN
N+ N+ ~~ a \ +
a WO 99/20627 _2~_ PCT/US98/22110 NH3+
NH3+ p ~N N+~~
N+ N+~ / U
H2N~ U H2N
~NH3+

~NH3+
~N N~~/ ~-N~+
H2N~ ~ H NI

~NH3+
NH3+ ~~./p NH~ H2N
N N+
-N N+~~
a ~ / ~/
~NH3+
/--\ NH2 NH3+ H N p ~- N+ N+~
N NI+~
U
NH3+ ~NH3+
~i ~i +.f b2+ ~- N N /-~ b2+
i~

Table iI

wherein Q-L-B is selected from:
I
\ /-'~ f--~1 \ n ~N N+ NH3+
~- N+ N+ N+~ ~ N+ N+
~/\ U\
\ ~ N~ NH3+
~N~+~ ~N+fNH3+ \ ~ J.-.N~
\ ~ N~+ ~N+~
\ ~ N N~ \ ~--~ N ~ IV
~N+ N+~ \-N+y-N+ N+-~ ~=N+~
\ ~--\ J- ~ 1 \ n 1 N+~~N+ ~- N+~ ~- N+ N+ ~ N- N+~
\ U
\ /-\ J---N ~ N
~N+ N+ '=N+f NH3+ ~ \ N+-U \ ~/ U ~

WO 99/20627 _22_ PCT/US98/22110 N\, N+~/NH3+
~N ~+ NU \
NH3+
N I N /~
N+~ NH3+ ~ _ ~ ~- N+~
N~ \
N ~.-/ ~
N
N ~ NON
N+~ ~ ~ ~ ~ N+~
N+ N+ ~ N ~ +
S
\/
~N ~/ ~ ~ N, N+~
~N ~ ~
~N
N
~N+~NH3+
-N ~+
_ NH3+
\ ~\ ~- N+'~
~N~iv ~

WO 99/20627 _Zg_ PCT/US98/22110 NH3+ - N+
\/
~- N+~~N \ N
N+~ , S N N+ ~ ~ N j '--~ ~ --N
n N~ N+
N+
N
\ ~--~ '~ I
~N~+ \~ ~N J
\ ~N ~--N~
-N+
n . /
~~ ~ + N+
~' ~' Nv /
~ ~+
w \ ~--y--~~N+ - ~ N+ N+ N+
~N ~N+ N~

WO 99/20627 _~_ PCT/US98/22110 NH3+
NH3+ O
~- N N+~
N+ N+~ I U
H2N~ ~.-/ H2N
.NH3+
/~/O
NH3+
~N N+ ~N~+
r NH3+
NH3+ O
NH~ H2N

N ~N+ - N N+~
~NH3+
N NNH~ NH3+ H2N O
/ N N~+~
U
NH3+ ,-NH3+
~ ~+

Table III
OH CH3 ~ ~ ~ Q-L-B
H H
_ ,Oz N
O

wherein Q-L-B is selected from:
\ /~ ~ ~ ~ n ~ N N+ N H3+
~- N+ N+ ~ N+~ ~ N ~N+
\-/~ \
N~ NH3+
N ~ + ~ ~ N+ f N H3+ \ ~--~ ~--.N
S N+~~N+ N+~
\ ~1 N N~ \ ~--~ N~ N
N+ N+ --~ \- N+w ~ N+ N+ -~ ~= N+~
~ y 1 \ ~\ vs1 ~.N~+-~~N+~ ~-N+ N+~ N-N+~
~N
~N N+~N~=N+f NH + ~ ~ N+

WO 99/20627 _2~ PCT/US98/22110 N\ N~ ~NH3+
\ Nl+~ _\ /~\
~N ~+ N~/ \
NH3+
N I N
\. N+~ NHg+ ~ _ ~ ~ N+~
N~ \
NU \
N
N, ~ N/~N
\ ~ ~ ~ N+~ ~ ~ ~ ~ N+~
N \~ ~ ~ N ~% +
\ S
\ ~\ ~' ~ \ 1 ~N ~/ ~ ~--1 N.N+~
~N ~ ~
~N
\ ~\ ~N
\, N+~NH3+
~N V+
_ NH3+
N+ N+ \
\ ~\ ~' N+~~
U \

WO 99/Z0627 _27_ PCTlUS98/22110 NH3+ -N+
\ n / ~N U \ N
N+~
N N+ ~ ~ Ng '-~ ~ 'N
n N~ N+
N+
N ~- N
\n ~N~+ \ ~ 'N J
\ ~N~N+
-N+
\/
~~ ~ + N+
~N \
~ ~+
\ n f--~~N+- ~ N+ N+ N+
N+~~N+ N-l a WO 99/20627 _~_ PCT/US98l22110 NH3+
NH3+ O
/ ~ N N+~
-N+ N+~ I U
H2N~ ~/ H2N
~NH3+
O
NH3+
-N~+ ~-N~+

NH3+
NH3+ O
HN
~NH
N+ N+~
~--/ ~ % ~i +
-NH3+-NH //
/--~ I ~ NH3+ H2N O
~~U+ n~ b ~~~+
~NH3+ ,--NH3+
2+ ~N N~~~2+
/ U
The process is illustrated by the following generic scheme:

WO 99/20627 _29_ PCT/US98/22110 FLOW SHEET A
p R~ 1) Activate hydroxyl group for displacement H H 2) React with side chain group H tR)z -X' O N
COZP"
N _~ ~R)z 1 ) Convert X' to X
2) Q-L-B, -L-B, 1 ) if necessary, modify R
2)deblock P and P"
3) If necessary, convert or deblock B' to the basic or cationic functionB
Steps 2 and 3 can be carried out separately, simultaneously or their order can be reversed.
/O -i-B
I /A
pH Rr ~~~ P =OH or hydroxyl protecting group H H N, ~R)z p~~ = carboxyl protecting grou = S p N ~
O X' = Precursor to X
X = a leaving group on A

Q' = monoalkylated monocationic precursor to the dialkylated, dicationic group p R , A, D, L and B as defined in the scope of the invention B'is a protected form of B, a precursorto B, or B' = B
With reference to Flow Sheet A above, P, R1, R, and M, are as defined with respect to the compounds of formula I.
5 P** represents a carboxyl protecting group.
~,1* represents a group which reacts with intermediate ~2 (upon activation of A~) in a manner which results in the incorporation in the final product of a member of the group defined as Q above, thus Q* may be viewed as a precursor for Q.

WO 99/20627 -3~- PCT/US98/22110 The naphthosultam side chain group used in the synthesis of the compounds of the present invention have, in some cases, been described in the chemical literature. In other cases, precursor compounds which may be readily converted to the requisite 5 naphthosultam have been described in the literature. In cases where the requisite naphthosultam is not known in the literature it is neceessary to synthesize the naphthosultam by a newly developed synthesis. One skilled in the art can adapt a previously published synthesis of an analogous naphthosultam to prepare the requisite 10 compound in a straightforward manner without undue experimentation. Examples of naphthosultam synthesis are described herein (see below).
The naphthosultam side chain group is initially reacted with a suitably protected carbapen-2-em-3-carboxylate having an 15 activated hydroxymethyl group at the 2-position.
The carbapenem nucleus having a -CH20H substituent at position 2 can be obtained in accordance with Schmitt, S. M. et al., J. Antibiotics 41(6): 780-787 (1988), the teachings of which are incorporated herein by reference. The carboxylic acid group at C-3 of 20 the carbapenem is generally protected as a carboxyl protecting group such as p-nitrobenzyl (PNB), allyl, p-methoxybenzyl, trichloroethyl, 2-trimethylsilylethyl, and the like. Furthermore, the hydroxyl group of the 6-(hydroxyethyl) side-chain is optionally protected with a hydroxyl protecting group such as trimethylsilyl (TMS), triethylsilyl (TES), 25 tent-butyldimethylsilyl (TBDMS), tert-butyldiphenylsilyl (TBDPS), acetyl, allyloxycarbonyl, 2-trimethylsilylethoxy carbonyl, 2-trichloroethoxycarbonyl and the like.
The addition of the naphthosultam side chain group to the carbapenem is accomplished by treating a solution of the 30 hydroxymethyl-carbapenem and the naphthosultam side chain group in a suitable solvent such as tetrahydrofuran (THF), ether, acetonitrile, dimethylformamide (DMF), benzene, dimethylsulfoxide (DMSO), and the like with a (premixed) suitable activating reagent such as diethyl azodicarboxylate (DEAD) / triphenylphosphine, 35 diisopropyl azodicarboxylate (DIAD) / tributylphosphine, and the like, at a temperature between about -20 °C and 35 °C for about 5 to minutes.
Alternatively, the naphthosultam and carbapenem can be mixed together with either the azodicarboxylate or the phosphine 5 reagent in a suitable solvent and the other component of the activating reagent (the phosphine or the azodicarboxylate, respectively) can be added to that mixture. Once the naphthosultam, carbapenem, and activating reagents) have been mixed, the reaction is allowed to proceed at a temperature between about -20 °C and 35 °C
IO for about 5 to 90 minutes.
The resulting mixture is then subjected to a standard work-up procedure familiar to those skilled in the art to afford a crude 2-naphthosultam-methyl substituted carbapenem which is purified, if necessary, by recrystallization or by chromatography on 15 silica gel, eluting with a suitable solvent or mixture of two or more solvents, such as hexane, ethyl acetate, ether, benzene, dichloromethane, chloroform, acetone, methanol and the like.
Modification of the naphthosultam side chain of compounds A2, which is generally necessary to introduce the 20 charged substituent of A4, is best accomplished before removal of the protecting groups. For compounds which contain a hydroxyl group (X') in the side chain, a positively charged substituent may be introduced into the side chain by first activating the hydroxyl group by converting it to a suitable leaving group (X) such as a triflate, 25 mesylate, tosylate, iodide, chloride, bromide, and the like, and then displacing the resulting leaving group with a compound Q*, such as a suitably substituted diazabicyclooctane or a suitably substituted N,N-dimethylpiperazine. Alternatively, in some cases, the charged substituent may be incorporated in the 30 naphthosultam side chain before addition of the naphthosultam to the carbapenem or may be introduced after deprotection of A2.
However, introduction of the charged substituent by modification of A2 before deprotection is greatly preferred.
In some cases, activation of the hydroxyl group and 35 displacement by Q* to produce A3 may be accomplished in a single step by taking advantage of the basic character of compound Q* and using it as a base in the activation reaction.
The conversion of the hydroxyl group to a suitable leaving group is accomplished by treating the hydroxyl substituted 5 compound in a suitable solvent such as dichloromethane, tetrahydro-furan, ether, benzene, and the like with an activating reagent, such as trifluoromethanesulfonic anhydride, methanesulfonic anhydride, toluenesulfonic anhydride, methanesulfonyl chloride, benzenesulfonyl chloride, toluenesulfonyl chloride, and the like in the 10 presence of a suitable base such as triethylamine, tributylamine, diisopropylethylamine, and the like at a temperature between about -100°C and 0°C for about 5 to 120 minutes. The intermediate thus obtained contains a leaving group, which may be converted to an alternative leaving group, iodide, by treating a solution of the 15 intermediate in a suitable solvent such as acetone, methyl ethyl ketone, and the like at about -10°C to 50°C with an excess of sodium iodide or potassium iodide for about 0.25 to 24 hours.
In many cases, the iodide is obtained in sufficiently pure form that it may be used without further purification. For ease of 20 handling, the iodide, if not crystalline, may be lyophilized from benzene to afford an amorphous, easily handled, solid.
The activated hydroxyl group or iodide is displaced by reacting the activated intermediate with reagent Q*. In some cases, activation and displacement of the hydroxyl group may be 25 accomplished in a single step. The activating reagent is added to a solution of the hydroxyl substituted compound in the presence of a suitable base in a suitable solvent such as dichloromethane, tetrahydrofuran, ether, DMF, benzene, acetonitrile, DMSO, and the like as described in the preceding paragraphs. The resulting 30 activated intermediate is treated with 1-3 molar equivalents of compound Q* at a temperature between about -78°C and 50°C for about 15 to 120 minutes. In some cases, it is desirable to form the activated intermediate in one solvent, isolate the activated intermediate, and conduct the displacement reaction in a different 35 solvent. In other cases, the displacement may be conducted without isolation of the intermediate and, in cases where Q* is also used as a base, may even be concurrent with the formation of the activated intermediate.
In cases where the displacement reaction is best accomplished by using the iodide, a solution of the iodide is combined with an approximately equivalent amount (0.9 - 1.05 molar equivalents) of compound f~*. A silver salt of a non-nucleophilic acid, such as silver trifluoromethanesulfonate, silver tetrafluoroborate and the like is then added. Although the reaction will proceed in the absence of the silver salt, the reaction proceeds more rapidly in the 10 presence of the silver salt. In addition, the silver salt assists in the removal of the displaced iodide from the reaction mixture which can improve the efficiency of subsequent steps. The resulting mixture is then subjected to a standard work-up procedure familiar to those skilled in the art to afford a crude product which is purified, if necessary, by recrystallization or chromatography.
The synthesis of the target compound is completed by removing any protecting groups which are present in the penultimate intermediate using standard techniques which are well known to those skilled in the art. The deprotected final product is 20 then purified, as necessary, using standard techniques such as ion exchange chromatography, HPLC on reverse phase silica gel, MPLC
on reverse phase polystyrene gel, and the like or by recrystallization.
The final product may be characterized structurally by standard techniques such as NMR, IR, MS, and UV. For ease of handling, the final product, if not crystalline, may be lyophilized from water to afford an amorphous, easily handled solid.
The compounds of the present invention are valuable antibacterial agents active against various Gram-positive and Gram-negative bacteria, and accordingly find utility in human and veterinary medicine.
Many of compounds of the present invention are biologically active against MRSA/MRCNS. In vitro antibacterial activity is predictive of in vivo activity when the compounds are administered to a mammal infected with a susceptible bacterial organism.

WO 99/20627 _g4_ PCT/US98/22110 Using standard susceptibility tests, the compounds of the invention are determined to be active against MRSA.
The invention is illustrated with the following non-limiting examples.

SYNTHESIS OF 4-(2-TRIETHYLSILArTYLOXY-ETHYL)-1 8 NAPHTHOSULTAM
~OH ~OAc \ AcCI / I \ 1 ) CIS03H
\ / Et3N \ / 2) K2C03 OAc OAc \ SOC12 / I \ HN03 \ / \
S03K ~ S02C1 _OAc -OH
/ \ NH3 / \ Cs2CO3 / \

~OH ~OTES
\ TESCI /
\ / \ /
HN-O,O HN-o.0 Sit n 1: 1-(2-Aceto~~vl)-naphthalene 10 Triethylamine (691 mL, 4.96 mol) was added to an ice cold solution of 1-(2-hydroxy-ethyl)-naphthalene (569 g, 3.30 mol) in dichloromethane (2.2 L). Acetyl chloride (282 mL, 3.97 mol) was added dropwise over 90 minutes. After the addition was complete, the reaction mixture was stirred for an additional 30 minutes with WO 99/20627 _35- PCT/US98/22110 ice-bath cooling. The reaction mixture was washed sequentially with water (2 x 1 L), 1N HCl ( 1 L, 500 mL), water ( 1 L), 5% aqueous NaHC03 (500 mL), water (I L), and brine (500 mL), then dried over magnesium sulfate, filtered, and evaporated to afford 1-(2-acetoxy-ethyl)-naphthalene (723.2 g) as a yellow oil that slowly crystallized.
tep 2: Potassium 4-(2-acetoxy-ethyl)-naphthalene-1-sulfonate Chlorosulfonic acid (69.3 g, 590 mmol) was added dropwise over 17 minutes to a solution of 1-(2-acetoxy-ethyl)-10 naphthalene (105.5 g, 490 mmol) in dichloromethane (200 mL). The reaction was exothermic and periodic ice-bath cooling was employed to maintain the internal temperature at 25-30°C. Approximately 10 minutes into the CLS03H addition, voluminous evolution of HCl gas was observed. After the addition was complete, the reaction mixture 15 was stirred at room temperature for 3 hours then cautiously added to ice (400 g). After shaking, the layers were separated. The aqueous layer was washed with dichloromethane then slowly neutralized by addition of a solution of potassium carbonate (77 g, 560 mmol) in water (200 mL). The precipitate was collected by filtration, washed 20 with cold water (100 mL), then dried under vacuum at 60 °C to afford potassium 4-(2-acetoxy-ethyl) naphthalene sulfonate (102.39 g) as a white solid. This material contained ca. 6% of the isomeric potassium 5-(2-acetoxy-ethyl)-1-naphthalene sulfonate as determined by 1H NMR. The filtrate was concentrated under vacuum to afford a 25 white suspension (355 g) which was stored in a refrigerator overnight. The solid was collected by filtration, washed with cold water (100 mL), then dried under vacuum at 60 °C to afford a second crop of potassium 4-(2-acetoxy-ethyl)-naphthalene-1-sulfonate (10.67 g) as a white solid. The second crop contained ca. 14% of the isomeric 30 potassium 5-(2-acetoxy-ethyl)-1-naphthalene sulfonate as determined by 1H NMR.
Sten 3: 4-(2-Acetoxv-eth lv )-naphthalene-1-sulfonvl chloride Potassium 4-(2-acetoxy-ethyl)-naphthalene-1-sulfonate 35 (102.3 g, 308 mmol) was added in portions over 15 minutes to a room temperature solution of dimethylformamide (2.4 mL, 31 mmol) in WO 99/20627 ~~ PCT/US98/22110 thionyl chloride (112 mL, 1.54 mol). The reaction mixture was gradually brought to 80 °C (oil bath temperature) over 30 minutes and heated at 80° C for 90 minutes, then cooled to room temperature and stirred at room temperature for 60 minutes. The reaction mixture 5 was partitioned between ice water (500 mL) and ethyl acetate (500 mL). The organic layer was washed with water (200 mL) and brine (200 mL), dried over magnesium sulfate, filtered, and evaporated under vacuum to a cream colored solid. The crude product was triturated with pet ether to afford 4-(2-acetoxy-ethyl)-naphthalene-1-sulfonyl chloride as a pale tan solid (77.47 g).
Step 4' 4-(2-Acetoxv-ethyl,}-8-vitro-naphthalene-1-sulfonyl chloride 4-(2-Acetoxy-ethyl)-naphthalene-1-sulfonyl chloride (76.96 g, 246 mmol} was added portionwise over 12 minutes to 90%
15 nitric acid (154 mL, 3.278 mol) cooled in an ice-methanol bath (-20°C).
After the addition was complete, the reaction mixture was stirred at -°C for an additional 15 minutes. The reaction mixture was partitioned between ice water (800 mL) and chloroform (800 mL). The aqueous layer was extracted with chloroform (100 mL). The 20 combined organic layers were washed with brine (400 mL, 200 mL), then dried over magnesium sulfate, filtered, and evaporated to a golden oil. Diethyl ether (300 mL) was added to the crude product and the mixure was shaken vigorously to afford an off white solid. The solid was collected by filtration, washed with ether (2 x 50 mL), and 25 dried under vacuum to afford 4-(2-acetoxy-ethyl)-8-vitro-naphthalene-1-sulfonyl chloride (41.85 g) as an off white solid.
Sten 5: 4-(2-Hvdroxv-ethyl)-.8-vitro-naphthalene-1-sulfonamide Solid 4-(2-acetoxy-ethyl)-8-vitro-naphthalene-1-sulfonyl chloride (39.64 g, 111 mmol) was added to an ice-cold, 6.8 M solution of ammonia in methanol (408 mL, 277 mmol). The cooling bath was removed, the reaction flask was stoppered, and the reaction was stirred at room temperature. After 4 days, the dark amber solution was concentrated under vacuum to a dark gum. The residue was 35 triturated vigorously shaken with water (300 mL) to give a solid which was washed with water ( 150 rnL) then ether ( 150 mL) and dried under vacuum. The resulting brown solid was recrystallized from isopropanol (300 mL) to afford 4-(2-hydroxy-ethyl)-8-nitro-naphthalene-1-sulfonamide (27.79 g) as tan flakes.
Sten 6: 4-(2-Hydroxv-ethyl)-1.8-naphthosultam Powdered cesium carbonate (76.8 g, 236 mmol) was added to a solution of 4-(2-hydroxy-ethyl)-8-nitro-naphthalene-1-sulfonamide (30.77 g, 94.3 mmol) in anhydrous dimethylformamide (470 mL). The mixture was placed under a nitrogen atmosphere, 10 sonicated for 10 minutes, then stirred at room temperature for 20 minutes. The mixture was then placed in a 100 °C oil bath and stirred vigorously. After 3.5 hours, the reaction mixture was removed from the heating bath, allowed to cool to room temperature, and left at room temperature overnight. The mixture was then 15 filtered and the collected solid was washed with dimethylformamide.
The combined filtrate and washings were evaporated to a dark oil.
This material was dissolved in water (400 mL), treated with activated charcoal (5 g), and the resulting mixture was heated on a hot water bath for 5 minutes. The mixture was cooled slightly then filtered 20 through a pad of super-cel. The filtrate was diluted with 2-butanone (450 mL), brine (300 mL), and 1M pH 1 aqueous phosphate (150 mL).
The mixture was shaken vigorously and the layers were separated.
The aqueous layer was extracted with 2-butanone (2 x I50 mL). The combined organic layers were washed with brine (2 x 300 mL), dried 25 over magnesium sulfate, filtered and evaporated to a brown solid (21.4 g). The solid was treated with ethyl acetate (100 mL), sonicated for 15 minutes and filtered. The collected solid was washed with cold ethyl acetate (50 mL) and dried under vacuum to afford 4-(2-hydroxy-ethyl)-1,8-naphthosultam as a pale brown powder (16.68 g).
30 IH NMR (DMSO-dg, 500 MHz) d 3.25 (t, ArC I~CH20H), 3.73 (m, ArCH2CH20H), 4.77 (t, ArCH2CH20I-~), 6.90 (d, H-7), 7.58 (dd, H-6), 7.69 (d, H-5), 7.69 (d, H-3), and 8.03 (d, H-2}.
Sten 7~ 4-(2-Triethy~silanvlox~~vl)-1 8-naphtho~ultam 35 Chlorotriethylsilane (13.57 mL, 80.86 mmol) was added dropwise over 1 minute to a vigorously stirred suspension of 4-(2-hydroxy-ethyl)-1,8-naphthosultam (17.53 g, 70.32 mmol) and imidazole (5.99 g, 87.90 mmol) in dichloromethane (351 mL). The reaction mixture was stirred under a nitrogen atmosphere at room temperature for 15 minutes, then water (350 mL) was added. The 5 organic layer vas washed sequentially with 0.2 N HCl (350 mL) and water (350 mL) then dried over magnesium sulfate, filtered, and evaporated under vacuum to a dark oil (29.07 g). The crude product was purified by flash column chromatography on silica gel (5 x 27 cm column, eluted with 4:1 hexanes-EtOAc followed by 3:1 hexanes-EtOAc) to afford a deep red oil (23.9 g). The oil was mixed with hexanes (225 mL), sonicated to start crystallization, and stirred at room temperature. The mixture was filtered and the collected solid was washed with hexanes (3 x 15 mL) and vacuum dried to afford 4-(2-triethylsilyloxy-ethyl)-1,8-naphthosultam (19.78 g) as a light pink-white solid.
1H NMR (CDC13, 500 MHz) d 0.54 (q, SiC~i2CHg), 0.88 (t, SiCH2C~g), 3.34 (t, ArCH2CH20), 3.95 (t, ArCH2C~-I20), 6.89 (d, H-7), 7.14 (s, NH), 7.50 (dd, H-6), 7.62 (d, H-3), 7.66 (d, H-5), 7.89 (d, H-2) mp 68.5-70.0°C

SYNTHESIS OF ALLYL (1S.5R,6S)-6-f(1R) (ALLYLOXYCARBONYLOXY)-ETHYLI-1-METHYL-2-(4-f2 (TRIFLUOROMETHANESULFONYLOXY)-ETHYLI-1,8 CARBOXYLATE

OTES

ROCO H H Me \
Me OH HN-S:O
" O
N O
O ~ PPh3 DIAD, PPh3 R = ailyl O
I I \
ROCO H H Me OTES heat ~
Me ~ \ /
N O \
O ~ PPh3 isy O O

-OTES
~OH
/ \
pll Me \ ~ / / ~ \
ROCO H H
Me N-S .O TtOH~ \ / Tf N~ O 'i,/N-S.O
O O

OTf / ~ \
O \ /
ROCO H H Me Me N SII~~O
O
O

~~vl (3-f1(R)-(all,~ycarbon~~)-ethyll-2-tl(R)-methyl-2-oxo-3-f4-(2-trieth ls~ ilanyloxv-eth lY )-1,8-naphthosultam~l_- rop 1~,35)-4-oxo-azetidin-1-yll-(trinhenylnhosphoranylidene)-acetate A solution of allyl (3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-[3-hydroxy-1(R)-methyl-2-oxo-propyl)-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (74.8 g, 116.2 mmol), 4-(2-triethylsilanyloxy-ethyl)-1,8-naphthosultam (38.03 g, 104.6 mmol), WO 99/20627 ~0_ PCT/US98/22110 and triphenylphosphine {45.72 g, 174.3 mmol) in anhydrous tetrahydrofuran (582 mL) was placed under a nitrogen atmosphere and cooled in an ice bath. Diisopropyl azodicarboxylate (34.3 mL, 174.3 mmol) was added over one minute. The resulting solution was 5 sirred at 0°C for 60 minutes followed by 30 minutes at room temperature. The mixture was filtered to remove a small amount of solid. The filtrate was diluted with ethyl acetate (300 mL) and washed with brine (300 mL). The organic phase was dried over magnesium sulfate, filtered and evaporated under vacuum to a dark red oil (220.1 10 g). The crude product was purified by chromatography on silica gel (5 kg), eluting with 7:3 hexane-ethyl acetate followed by 1:1 hexane-ethyl acetate. The product containing fractions were combined and evaporated under vacuum to afford the title compound (94.7 g) as a viscous oil. In order to determine an accurate yield for the reaction, 15 an aliquot of this material (508.8 mg of 263.5 g of a solution of the product in toluene) was lyophilized from benzene to afford an off white fluffy solid (144.1 mg). The actual yield of product was calculated as 74.62g.
20 Step 2' ,All, 1~ (1S,5R,6S)-6-((1R)-fallyloxycarbonyloxy)-ethyll-1-methyl-2- f 4-( 2-triethylsilanvl oxv-ethyl)-1.8-naphthosultamvl-methyll -carbapen-2-em-3-carboxvlate A solution of allyl [3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-{ 1(R)-methyl-2-oxo-3-[4-(2-triethylsilanyloxy-ethyl)-1,8-25 naphthosultamyl]-propyl)-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (74.6 g, 75.4 mmol, see step 1) in toluene (750 mL) was placed under a nitrogen atmosphere and refluxed for 2.5 hours. The reaction mixture was allowed to cool to room temperature and evaporated to a paste which was stored 30 overnight in the freezer as a suspension in dichloromethane. The crude product was purified by chromatography on silica gel (1.8 kg), eluting with 85:15 hexane-ethyl acetate followed by 4: hexane-ethyl acetate. The product containing fractions were combined and evaporated under vacuum to afford the title compound (42.4 g) as a 35 viscous tan oil.

WO 99/20627 ..,41_ PCT/US98/22110 IR (thin film on NaCl) 2955, 2876, 1784, 1746, 1716, 1258, and 661 cm-1 IH NMR (CDC13, 500 MHz) d 0.52 (q, SiCH2CH3), 0.88 (t, SiCH2CI~- 3) 1.29 (d, 1-CH3), 1.43 (d, C~gCHOC02), 3.34 (t, 5 ArCH2CH20), 3.37 (dq, H-1), 3.43 (dd, H-6), 3.94 (t, ArCH2C~0), 4.14 (dd, H-5), 4.56-4.60 (m, OC02CH2), 4.67 (d, 2-CHaHb), 4.81 and 4.92 (two dd, C02CH2), 5.11 (dq, CH3CHOC02), 5.24 (d, vinyl-H) 5.30-5.34 (m, 2 vinyl-H), 5.37 (d, 2-CHa~), 5.50 (d, vinyl-H), 5.86-5.89 (m, vinyl-H), 6.00-6.04 (m, vinyl-H), 6.69 (d, Ar H-7), 7.50 (t, Ar H-6), 7.61 (d, Ar 10 H-5), 7.63 (d, Ar H-3), 7.90 (d, Ar H-2) HRMS (FAB) calculated for C36H47N2OgSiS (MH+) 711.2771; found 711.2690.
Step 3' Allvl (1S,5R 6S)-6-f(1R)-(allyoxycarbonvloxy)-ethvll-2-f4-(2-15 hvdrox~ roRyl)-1,8-na~hthosultamvl-methvll-1-methyl-carba ep n-2-em-3-carboxvlate A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-1-methyl-2-[4-(2-triethylsilanyloxy-ethyl)-1,8-naphthosultamyl-methyl]-carbapen-2-em-3-carboxylate (45.2 g, 20 63.6 mmol) in tetrahydrofuran (500 mL) was diluted with water (125 mL) and treated with 1M aqueous trifluoromethanesulfonic acid (6.4 mL, 6.4 mmol). The resulting mixture was stirred at room temperature for 15 minutes, then partitioned between 5% aqueous sodium bicarbonate (200 mL), brine (25 mL), and ethyl acetate (500 25 mL). The organic phase was washed with dilute brine (200 mL), dried over magnesium sulfate, filtered and evaporated under vacuum to an oil (50.2 g). The oil was evaporated under vacuum from toluene (3 x 50 mL) to afford the title compound (42.9 g) as a tacky foam. This material was used without purification in the next step.
30 1H NMR (CDC13) d 1.31 (d, 1-CH3), 1.45 (d, C i~3CHOC02), 3.41 (t, ArCH2CH20H), 3.41 (dq, H-1), 3.45 (dd, H-6), 4.04 (dt, ArCH2CH20H), 4.16 (dd, H-5), 4.59 (m, OC02CH2), 4.69 (d, 2-C -~aHb), 4.89 (m, C02CH2), 5.14 (dq, CH3C~- OC02), 5.26, 5.33, 5.35 and 5.53 (four m, 4 vinyl-H), 5.41 (d, 2-CHaHb), 5.91 and 6.05 (two m, 2 35 vinyl-H), 6.73 (d, Ar H-7), 7.54 (dd, Ar H-6), 7.63 (d, Ar H-5), 7.68 (d, Ar H-3), 7.95 (d, Ar H-2) WO 99/Z0627 ,~2- PCT/US98/22110 Sten 4: Allyl (1S.5R.6S)-6-f(1R)-(allyloxvcarbonvloxy)-ethyll-1-meth ~~l-2-(4-f2-(trifluoromethanesulfonylo~c )-et 11-1,8-nanhthosultam ~~l-methyll-carbapen-2-em-3-carboxvlate 5 A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-2-[4-(2-hydroxy-propyl)-1,8-naphthosultamyl-methyl]-1-methyl-carbapen-2-em-3-carboxylate (63.58 mmol, amount teoretically present in the unpurified product of the previous step) in anhydrous methylene chloride (750 mL) was 10 placed under a nitrogen atmosphere, cooled in an ice-methanol bath (-15°C), and treated with 2,6-lutidine (22.2 mL, 190.7 mmol). The resulting mixture was aged at -15°C for 8 minutes then trifluoromethanesulfonic anhydride (16.0 mL, 95.4 mmol) was added over a period of 4 minutes. After 30 minutes at -15°C, the reaction 15 mixture was diluted with methylene chloride (1.25 L) and washed sequentially with water (1.8 L), 0.2N hydrochloric acid (2 x 1.8 L) and water (1.8 L), then dried over magnesium sulfate, filtered, and evaporated under vacuum to give the title compound as an oil.
1H NMR (CDC13, 500 MHz) 8 1.31 (d, 1-C~- 3), 1.44 (d, 20 C~CHOH), 3.39 (m, H-1), 3.45 (dd, H-5), 3.67 (CHzCH20Tf), 4.16 (m, H-6), 4.58 (t, CHZOTfj, 4.67 (d, 2-CHaHb), 4.82 (m, CHZCH=CH2), 4.93 (m, CH2CH=CH2), 5.12 (m, CI-~CH30), 5.24 (d, C~- 2CH=CH2), 5.32 (m, CH2CH=CH2), 5.41 (d, CH2N), 5.52 (d, CHZCH=CHZ), 5.88 (m, CHZCH=CHZ), 6.03 (m, CHZC~=CH2), 6.77 (d, ArH-7), 7.54 and 7.58 (2 25 m's, ArH-5 and Ar H-6), 7.67 (d, Ar H-2), 7.96 (d, Ar H-4).

SYNTHESIS OF 3-(2-TRIETHYLSILArTYLOXY-ETHYL)-1,8 NAPHTHOSULTAM

WO 99/20627 ,.~_ PCT/US98/22110 Br / I \ ~ _ NBA / I \ ~ AcC
\ / OH \ / OH Et3N
Br Br / \ ~ 1 ) CIS03H / \ ~ SOC12 \ I / OAc 2) K2CO3 \ ( / OAc Br Br \ ~ -HN~ / I \ ~ NH
\ / OAc \ / OAc Br Br / I \ 1 Nab / I \ ~ Cs2- CO~
\ / OAc \ / OH

Br / \ ~ H2 / \ TESCI
\ I / OH Pd(OH)2/C ( \ / OH
HN-OHO HN-O'O
/ \
\ I / OTES
HN-~.O
O
Stew 1 ~ 1-Bromo-2-(2-hvdroxy-eth lv )-naphthalene WO 9920627 ~- PCT/US98/22110 A solution of 2-(2-hydroxy-ethyl)-naphthalene (58.5 g, 0.34 mol) in anhydrous acetonitrile (500 mL) was treated with N-bromo-succinimide (66.5 g, 0.37 mol). The resulting solution was stirred at room temperature under a nitrogen atmosphere and 5 protected from light for 30 minutes, then heated in an oil bath at 50°C
for 2 hours. After cooling to room temperature, the reaction mixture was evaporated under vacuum to a viscous oil. The oil in diethyl ether (350 mL) was washed with water (350 mL}, dilute aqueous sodium thiosulfate (300 mL), water (300 mL) and brine (200 mL), 10 dried over magnesium sulfate, filtered, and evaporated under vacuum to an oil (89.5 g) that solidified on standing. The crude product was chromatographed on a column of EM silica gel 60, eluting with methylene chloride, to afford a yellow solid (78.2 g).
Recrystallization of this material from carbon tetrachloride provided 15 the title compound (46.5 g) as a pale yellow solid.
Step 2: 2-(2-Acetoxy-ethyl)-1-bromo-naphthalene A solution of 1-bromo-2-(2-hydroxy-ethyl)-naphthalene (46.5 g, 0.185 mol) in methylene chloride (370 mL) was placed under a 20 nitrogen atmosphere, cooled in an ice bath, and stirred.
Triethylamine (32.3 mL, 0.232 mol) was added followed by acetyl chloride (15.8 mL, 0.222 mol) dropwise over 5 minutes. The reaction mixture was removed from the ice bath and stirred at room temperature for 15 minutes. The reaction mixture was washed with 25 water (300 mL), 1N hydrochloric acid (200 mL) and water (250 mL), dried over magnesium sulfate, filtered, and evaporated under vacuum to afford the title compound as an oil (55.1 g).
Sten 3' Potassium 3-(2-aceto~c ~-ethyl)-4-bromo-naphthalene-1-30 nat A solution of 2-(2-acetoxy-ethyl)-1-bromo-naphthalene (32.5 g, 0.111 mol) in trifluoroacetic acid (112 mL) was stirred under a nitrogen atmosphere and cooled in an ice bath while chlorosulfonic acid (8.9 mL, 0.130 mol) was added dropwise over 5 minutes. The 35 resulting solution was heated in an oil bath at 50°C for 90 minutes then cooled to room temperature and evaporated under vacuum to WO 99/20627 ~5_ PCT/US98/22110 dark oil. The oil was partitioned between methylene chloride (150 mL) and water (150 mL). The aqueous phase was washed with methylene chloride (150 mL), briefly pumped under vacuum, then brought to pH 8 with 3M aqueous potassium hydroxide (30 mL) 5 followed by 4M aqueous potassium carbonate (35 mL). The resulting mixture was stirred in a cold room (5°C) for 2 hours and filtered to remove the product. The recovered white solid was vacuum dried to afFord the title compound (11.21 g).
10 ten 4~ 3-(2-Acetoxy-ethyl)-4-bromo-naphthalene-1-sulfon~rl chloride Potassium 3-(2-acetoxy-ethyl)-4-bromo-naphthalene-1-sulfonate (17.75 g, 43.2 mmol) was added at room temperature to a stirred solution of N,N-dimethylformamide (0.334 mL, 4.31 mmol) in thionyl chloride (63 mL, 863 mmol). The resulting mixture was 15 placed in an oil bath at 70°C and stirred. After 10 minutes, additional thionyl chloride (20 mL) was added to facilitate stirring. After 40 minutes at 70°, the reaction flask was fitted with a distillation head and excess thionyl chloride was removed under vacuum. The residual brown solid was mixed with diethyl ether (300 mL) and 20 added to an ice-cold, stirred mixture of water (I00 mL) and ether (100 mL). The organic phase was separated, washed with water (200 mL) and brine (100 mL), dried over magnesium sulfate, filtered, and evaporated under vacuum to afford the title compound (14.63 g).
25 Step 5' 3-(2-Acetoxv-ethyl)-4-bromo-8-nitro-naphthalene-1-sulfonXl chloride An ice-cold, stirred solution of 3-(2-acetoxy-ethyl)-4-bromo-naphthalene-1-sulfonyl chloride (17.66 g, 45.1 mmol) in trifluoroacetic acid (150 mL) was treated with 96% sulfuric acid (12.5 30 mL, 225 mmol) and with 90% nitric acid (2.65 mL, 56.4 mmol) added dropwise over 3 minutes. The reaction mixture was removed from the ice bath, stirred at room temperature for 15 minutes, re-cooled in an ice bath, and treated with water (850 mL) added dropwise. The resulting mixture was filtered through a celite pad to collect the solid 35 which was washed with water (100mL) and dissolved in methylene chloride (350 mL). The methylene chloride solution was washed with WO 99120627 ~6- PCTNS98/22110 water (500 mL) containing brine (100 mL), dried over magnesium sulfate, and evaporated under vacuum to an oil (21.23 g). This material was shown to be a 42:58 mixture of the 5-N02 to 8-N02 products by 1H NMR}. The crude product was mixed with ethyl 5 acetate (20 mL) and sonicated to provide a crystalline precipitate.
This material was collected, washed with ethyl acetate, and dried under vacuum to afford the title compound (8.llg) as an off white solid. The mother liquors yielded an additional 1.47 g of the title compound following flash chromatography on silica gel (eluting with 10 30-35% ethyl acetate in hexane) and crystallization from diethyl ether.
Step 6: 3-(2-Acetoxv-ethyl)-4-bromo-8-vitro-naphthalene-1-sulfonamide Solid 3-(2-acetoxy-ethyl)-4-bromo-8-vitro-naphthalene-1-15 sulfonyl chloride (5.00 g, 11.45 mmol) was added at room temperature to 0.5M ammonia in dioxane (92 mL, 46 mmol). After stirring at room temperature for 40 minutes, the mixture was evaporated under vacuum to a residue which was mixed with water (100 mL), sonicated, and filtered. The collected pale-yellow solid was washed 20 with water (2 x 20 mL) and vacuum dried to afford the title compound (4.75 g).
Step 7: 4-Bromo-3-(2-hydroxy-ethyl)-8-vitro-naphthalene-1-sulfonamide 25 Sodium methoxide in methanol (23.7 mL of a 0.5M
solution, 11.8 mmol) was added to a suspension of 3-(2-acetoxy-ethyl)-4-bromo-8-vitro-naphthalene-1-sulfonamide (4.70 g, 11.3 mmol) in methanol (33 mL}. The mixture was stirred under a nitrogen atmosphere at room temperature for 90 minutes, then concentrated 30 under vacuum to approximately half volume, diluted with ethyl acetate (200 mL), and washed with 2N hydrochloric acid. The oganic solution was washed with water (100 mL) and brine (50 mL), dried over magnesium sulfate, filtered, and left to stand at room temperature. The organic solution deposited a solid which was 35 collected by filtration, washed with ethyl acetate (2 x 15 mL), and vacuum dried to give the title comound ( 1.78 g). Additional poduct WO 99/20627 ~7_ PCT/US98/221I0 { 1.88g) was obtained from the mother liquors after concentration under vacuum and crystallization from diethyl ether.
~n 8' 4-Bromo-3-(2-hydrox -y eth ly )-1,8-nanhthosultam 5 A solution of 4-bromo-3-(2-hydroxy-ethyl)-8-nitro-naphthalene-1-sulfonamide (3.61 g, 9.62 mmol) in anhydrous N,N-dimethylformamide (96 mL) was treated with cesium carbonate (7.84 g, 24.1 mmol). The resulting mixture placed under a nitrogen atmospere, sonicated at room temperature for 10 minutes, stirred at 10 room temperature for 5 minutes, and then heated in an oil bath at 100°C for 2 hours. The mixture was evaporated under vacuum to a brown residue which was partitioned between ethyl acetate (100 mL) and 2N hydrocloric acid (20 mL). The organic phase was washed with water (20 mL) and brine (20 mL), dried over magnesium sulfate, 15 filtered, and evaporated under vacuum to a solid (2.83 g). This material was mixed with diethyl ether (30 mL), sonicated, stirred, and filtered. The collected solid was washed with ether (20 mL) and vacuum dried to give the title compound (2.21 g) as a tan powder.
20 step 9: 3-(2-Hydro~-ethyl)-1,8-naphthosultam A solution of 4-bromo-3-(2-hydroxy-ethyl)-1,8-naphthosultam (2.10 g, 6.4 mmol) in ethanol (105 mL) was treated with triethylamine (2.68 mL, 19.2 mmol) and 20% palladium hydroxide on carbon (0.84 g). The mixture was hydrogenated (45-50 25 psi H2) on a Parr shaker for 6.5 hours at room temperature, then filtered through a celite pad to remove the catalyst which was washed with additional ethanol (3 x 5 mL). The filtrate and washings were evaporated under vacuum to a residue which was partitioned between ethyl acetate (60 mL) and 1N hydrochloric acid (50 mL). The 30 organic phase was washed with brine (25 mL), dried over magnesium sulfate, filtered, and evaporated under vacuum to afford the title compound (1.32 g) as a brown solid.
~tgp 10' 3-(2-Triethylsilanyloxy-eth~1 8-nanhthosultam 35 A mixture of 3-(2-hydroxy-ethyl)-1,8-naphthosultam (1.44 g, 5.78 mmol) and imdazole (0.495 g, 7.27 mmol) in anhydrous WO 99/20627 "~- PGT/US98~22110 methylene chloride (39 mL), at room temperature and under a nitrogen atmosphere, was treated with chlorotriethylsilane (1.12 mL, 6.69 mmol). After stirring at room temperature for 30 minutes, the mixture was diluted with methylene chloride (60 mL), washed with 5 water (I00 mL), 0.2N hydrochloric acid (50 mL) and water (100 mL), dried over magnesium sulfate, filtered, and evaporated under vacuum to a dark oil (2.15 g). The oil was purified by flash chromatography on EM silica gel 60 (4 x 15 cm column), using 3:1 hexane-ethyl acetate as eluting solvent, to give an oil (2.09 g). This 10 material was mixed with hexane ( 10 mL) and sonicated to afford a crystalline solid. The solid was collected, washed with hexane (3 mL), and dried to afford the title compound (1.73 g).
MP. 97.5-98.0°C.
1H NMR (CDC13) d 0.54 (q, SiC i~CHg), 0.88 (t, SiCH2C~-I3), 3.08 15 (t, ArCH2), 3.90 (t, CH20), 6.84 (m, H-7), 7.24-7.47 (m, H-5 and H-6), 7.84 and 7.90 (two d's, H-2 and H-4).

SYNTHESIS OF ALLYL (1S Rf6S)-6-f(1R) 20 (ALLYLOXYCARBQNYLOXY)-ETHYLI-1-METHYL-2-(3-f2-(TRIFLUOROMETHANESULFONYLOXY)-ETHYLI-1~8-CARBOXYLATE

WO 99/20627 ~9- PCT/US98/22110 ~I
ROC H H Me ~ / OTES
Me ~ OOH HN-S:O
O O
O ~ PPh3 DIAD, PPh3 R = allyl Q
ROC Me I \
H H
w Me / heat N O N\ \
O ~ PPh3 iSv OTES
O O

/ \
ROC \ I / OTES / \
H H Me I
Me N-S~~O TfO~ \ / OH Tf N~ O ~~N S~~O
O O

/ \
ROC \ I / OTf H H Me )' Me N
/j-" O
~'~(O
COZR
Step 1Allvl (3-f 1(R)-(al~,vlo~cycarbonylox )-et rll-2-11(R)-methyl-2-oxo-3-f3-(2-trieth ly silanyloxy-eth 1~ 8-na~hthosultamyll-prop, 1~,, 4-oxo-azetidin-1-yll-(tripheny_lphosphoranylidene)-acetate A solution of allyl {3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-[3-hydroxy-1(R)-methyl-2-oxo-propyl]-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (3.81 g, 5.91 mmol), 3-(2-triethylsilanyloxy-ethyl)-1,8-naphthosultam (1.72 g, 4.73 mmol), and triphenylphosphine (2.32 g, 8.87 mmol) in anhydrous WO 99/20627 -5~- PCT/US98/22110 tetrahydrofuran (30 mL) was placed under a nitrogen atmosphere and cooled in an ice bath. Diisopropyl azodicarboxylate (1.75 mL, 8.$7 mmol) was added over one minute. The resulting solution was sirred at 0°C for 60 minutes followed by 30 minutes at room temperature, 5 then diluted with ethyl acetate (100 mL) and washed with brine (50 mL). The organic phase was dried over magnesium sulfate, filtered and evaporated under vacuum to a viscous, dark oil. The crude product was purified by flash chromatography on EM silica gel 60 (5 x 19 cm column), eluting with 2:1 hexane-ethyl acetate. The product containing fractions were combined and evaporated under vacuum to afford the title compound (3.48 g) as a foam.
Step 2: Allyl (1S.5R,6S)-6-f(1R)-(all~vcarbonvlox~~)-ethyll-1-methxl-2-[3-(2-triethylsilanyloxv-eth lv )-1,8-naphthosultamvl-methyll-carbapen-2-em-3-carboxylate A solution of allyl {3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-{1(R)-methyl-2-oxo-3-[3-(2-triethylsilanyloxy-ethyl)-1,8-naphthosultamyl]-propyl)-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (3.47 g, 3.50 mmol) in 20 anhydrous toluene (35 mL) was placed under a nitrogen atmoshere and heated at reflux for 3.5 hours. The solution was concentrated under vacuum to 10 mL and applied to a column (4 x 20 cm) of EM
silica gel 60. The column was eluted with 3:1 hexane-ethyl acetate (28 x 20 mL fractions) followed by 2:1 hexane-ethyl acetate (20 mL
25 fractions). The product containing fractions were combined and concentrated under vacuum. The residue was lyophilized from benzene to give the title compound (1.894 g) as a gum.
1H NMR (CDCl3) d 0.57 (q, SiCH2CH3), 0.92 (t, SiCH2C~,3)~ 1.31 (d, 1-CH3), 1.45 (d, CH3CHOC02), 3.11 (t, 30 ArC i~CH20), 3.41 (dq, H-i), 3.45 (dd, H-6), 3.93 (t ArCH2C~0), 4.17 (dd, H-5), 4.59 (m, OC02CH2), 4.67 (d, 2-CHaHb), 4.81-4.95 (m, C02CH2), 5.14 (dq, CH3C -~iOC02), 5.25-5.36 (m, 3 vinyl-H), 5.39 (d, 2-CHaH~), 5.50-5.54 (m, vinyl-H), 5.88-5.93 (m, vinyl-H), 6.01-6.07 (m, vinyl-H), 6.66 (d, Ar H-7), 7.43 (d, Ar H-5), ?.48 (dd, Ar H-6), 7.90 and 35 7.93 (two s, Ar H-2 and Ar H-4).

Step lyl_(_1_S_ R,6S)-6-f(1R)-(allyloxycarbonyloxv)-ethxll-2-f3-(2-h droxv-Rropyl)-1.8-naphthosultamvl-methvll-1-methyl-carba ep n-2-em-3-carboxylate A solution of allyl (1S,5R,6S)-6-[(1R)-5 (allyloxycarbonyloxy)-ethyl]-1-methyl-2-[3-(2-triethylsilanyloxy-ethyl)-1,8-naphthosultamyl-methyl]-carbapen-2-em-3-carboxylate (0.502 g, 0.706 mmol) in tetrahydrofuran (5.6 mL) was diluted with water (1.4 mL) and treated with 1M aqueous trifluoromethanesulfonic acid (0.071 mL, 0.071 mmol). After stirring at room temperature for 15 10 minutes, the solution was treated with 2% aqueous sodium bicarbonate (5 mL) and extracted with ethyl acetate (25 mL). The organic phase was washed with brine (25 mL), dried over magnesium sulfate, filtered and evaporated under vacuum to provide the title compound (0.473 g) as an oil.
15 1H NMR (CDCl3) d 1.31 (d, 1-CH3), 1.45 (d, CH3CHOC02), 3.16 (t, ArCH2CH20H), 3.40 (dq, H-1), 3.45 (dd, H-6), 4.01 (q, ArCH2C~20H), 4.16 (dd, H-5), 4.59 (m, OC02CH2), 4.67 (d, 2-CH~Hb), 4.81-4.95 (m, C02CH2), 5.13 (dq, CH3CHOC02), 5.25-5.36 (m, 3 vinyl-H), 5.40 (d, 2-CHaI~), 5.50-5.54 (m, vinyl-H), 5.88-5.93 (m, 20 vinyl-H), 6.02-6.07 (m, vinyl-H), 6.68 (d, Ar H-7), 7.45 (d, Ar H-5), 7.50 (dd, Ar H-6), 7.91 and 7.96 (two s's, Ar H-2 and Ar H-4).
Step 4: All 1~,5R~6S)-6-f(1R)-(all loxycarbonvloxy)-ethyll-1-methyl-2-(3-f2-(trifluoromethanesulfon~oxy)-eth 1~,8-naphthosultamyl-25 methyll-carbapen-2-em-3-carboxylate A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-2-[3-(2-hydroxy-propyl)-1,8-naphthosultamyl-methyl]-I-methyl-carbapen-2-em-3-carboxylate (0.706 mmol) and 2,6-lutidine (0.246 mL, 2.12 mmol) in anhydrous 30 methylene chloride (9.4 mL) was placed under a nitrogen atmosphere and cooled in a CC14-dry ice bath (-23°C).
Trifluoromethanesulfonic anhydride (0.178 mL, 1.06 mmol) was added and the resulting solution was stirred in the cold for 30 minutes. The solution was diluted with methylene chloride (20 35 mL) and water (20 mL) and shaken. The organic phase was separated, washed with 0.2N hydrochloric acid (20 mL) and water (20 mL), dried over magnesium sulfate, filtered and evaporated under vacuum to give the title compound as a foam (0.531 g).
1H NMR (CDC13) d 1.32 (d, 1-CHg), 1.46 (d, C~3CHOC02), 3.41 (dq, H-1), 3.44 (t, ArCH2CH20), 3.45 (dd, H-6), 4.18 (dd, H-5), 4.56-4.64 (m, OC02CH2), 4.63 (d, 2-CH~Hb), 4.83 (t, ArCH2CH20), 4.8-4.96 (m, C02CH2), 5.14 (dq, CH3CHOC02), 5.25-5.36 (m, 3 vinyl-H), 5.42 (d, 2-CHaHb), 5.51-5.54 (m, vinyl-H), 5.86-5.95 (m, vinyl-H), 6.02-6.09 (m, vinyl-H), 6.73 (d, Ar H-7), 7.48 (d, Ar H-5), 7.55 (dd, Ar H-6), 7.86 and 7.97 (two s's, Ar H-2 and Ar H-4).

SYNTHESIS OF 4-(TRIETHYLSILYLOXY-METHYL)-1 8 NAPHTHOSULTAM
Br Br Br / ~ \ ClS03H~ / I \ HNO~ / I \ SnCl2-\ / \ / \ /

Br Br CHO
\ P~ / I \ BuLi~ / ( \ Nab \ / \ / HC02Et \ /
NH2 S03Na H,N-O,O H,N-O ~
OH OTES
\ TESCI /
\ / ~ \ /
H'N ~'~O H'N 0~0 Step 1: Potassium 4-bromo-naphthalene-1-sulfonate A solution of 1-bromonaphthalene (19 mL, 137 mmol) in carbon tetrachloride (24 mL) was cooled in an ice bath under nitrogen. Chlorosulfonic acid (9.1 mL, 137 mmol) was added dropwise over 20 minutes. After an additional 5 minutes, the heavy wo ~no62~ -53_ PCT/US98/22110 grey suspension was removed from the ice bath and was stirred at room temperature for 16 hours to give a grey paste. The mixture was partitioned between methylene chloride (100 mL) and water (300 mL).
The aqueous layer was made basic with potassium carbonate and the 5 resulting suspension was filtered. The collected solid was washed with methylene chloride (50 mL) and water (50 mL), and dried under vacuum to give the title compound as a white solid (30 g).
1H NMR (DMSO-d6) d 7.61 (m, ArH), ?.65 (m, ArH)~ 7.82 (m, 2AxH), 8.14 (dd, ArH), and 8.90 (dd, ArH).
Step 2: 4-Bromo-8-nitro-naphthalene-1-sulfonic acid Potassium 4-bromo-naphthalene-1-sulfonate (1.38 g, 4.24 mmol) was added portionwise over 20 minutes to 90% nitric acid (2 mL), which was cooled in a methanol/ ice bath to approximately -15 15 °C. After 1.5 hours, the mixture was placed in a refrigerator for 20 hours. Diethyl ether (20 mL) was added and the precipitated solid was filtered, washed with ether (I00 mL) and isopropanol (20 mL), and dried under a stream of nitrogen to give the title compound as an approximately 4:1 mixture of the 5- and 8-nitro isomers (1.25 g).
1H NMR (D20) d 7.70 (dd, ArH), 8.09 (d, ArH), 8.20 (d, ArH)~ 8.21 (dd, ArH), and 8.63 (d, ArH).
ten 3: Sodium 4-bromo-8-amino-naphthalene-1-sulfonate 4-Bromo-8-nitro-naphthalene-1-sulfonic acid (1 g, 3.01 25 mmol) and tin chloride dehydrate (1.83 g, 8.I mmol) were suspended in a mixture of water (10 mL) and ethanol (10 mL). The resulting mixture was heated for 3 hours in a 100 °C oiI bath. The mixture was cooled to room temperature and filtered. The collected solid was suspended in water (20 mL) and the mixture was made basic with 30 sodium carbonate then placed on a CG-161 amberchrom resin column (3 x 9 cm). The column was washed with water (300 mL) and was eluted with 25% MeCN/ H20, collecting 12 mL fractions.
Fractions 17-19 were combined and evaporated to give the title compound as a solid (0.33 g).
35 1H NMR (D20) d 7.07 (dd, ArH), 7.49 (t, ArH)~ 7.83 (d, ArH), 7.85 (dd ArH) and 8.08 (d, ArH).

Step 4: 4-Bromo-1.8-naghthosultam Sodium 4-bromo-8-amino-naphthalene-1-sulfonate (1.2 g, 3.70 mmoi) was suspended in phosphorous oxychloride (10 mL, 107 5 mmol) and the mixture was refluxed for 1 hour to give a thin suspension. The mixture was cooled to room temperature and was added to ice (100 mL). The precipitate was collected and washed with water (20 mL) then dried under vacuum (0.675 g). A second crop was obtained from the filtrate (0.186 g). The combined solids were 10 dissolved in 5% methanol in methylene chloride and were placed on a silica gel column (29 x 3.5cm, packed and eluted with 5% methanol in methylene chloride), collecting 8 mL fractions. Fractions 27-39 were combined and evaporated to give the title compound as a solid (0.55 g).
1H NMR (0.14 mL CDC13 and 0.01 rnL CD30D) d 6.89 (d, 15 ArH), 7.58(dd ArH), 7.68 (d, ArH), 7.73 (d, ArH) and 7.95 (d, ArH).
Step 5: 4-Formvl-1,8-naphthosultam A solution of 4-bromo-1,8-naphthosultarn (0.24 g, 0.845 mmol) in anhydrous tetrahydrofuran (5 mL) was cooled in a dry ice/
20 acetone bath under nitrogen. n-Butyllithium (1.32 mL of a 1.6 M
solution in hexanes, 2.11 mmol) was added and the mixture was stirred for 5 minutes. Ethyl formate (1 mL, 12.4 mmol) was then added, and after an additional 5 minutes, 2N aqueous hydrochloric acid (3 mL) was added. The flask was removed from the bath and the 25 yellow solution was partitioned between ethyl acetate (30 mL) and water (30 mL). The ethyl acetate layer was washed with saturated aqueous sodium chloride (20 mL), dried over magnesium sulfate, filtered, and evaporated. The residual oil was purified on preparative silica gel plates (3 x 1000 micron/ developed and eluted with 5%
30 methanol/ methylene chloride) to give the title compound as a red solid (0.035 g).
1H NMR (CDCl3) d 7.09 (d, ArH), 7.78 (dd, ArH)~ 8.12 (d, ArH), 8.30(d, ArH), 8.70 (d, ArH) and 10.5 (s, CHO).
35 step 6: 4-H dy rox~~ethvl-1.8-nanhthosultam WO 99/20627 -55_ PGT/US98/22110 A solution of 4-formyl-1,8-naphthosultam (0.035 g, 0.15 mmol) in anhydrous methanol (1 mL) was cooled in an ice bath under nitrogen. Sodium borohydride (0.011 g, 0.3 mmol) was added and the solution was stirred for 30 minutes. The mixture was 5 partitioned between methylene chloride (10 mL) and 0.2N aqueous hydrochloric acid (10 mL). The aqueous layer was extracted with 5%
methanol in methylene chloride (2 x 10 mL), and the combined organic layers were evaporated to give the title compound as a yellow solid (0.032 g).
10 1H NMR (0.14mLCDCl3and O.OlmL CD30D) d 5.13 (s, C~i20H), 6.85 (d, ArH), 7.50 (dd, ArH), 7.57 (d, ArH), 7.82 (d, ArH) and 7.88 (d, ArH).
4-(Triethvlsilvloxv-methvl)-1,8-nanhthosultam 15 A mixture of 4-hydroxymethyl-1,8-naphthosultam (2.92 g, 8.7 mmol), imidazole (0.74 g, 10.9 mmol), and triethylsilyl chloride (1.68 mL, 10.0 mmol) in dichloromethane (40 mL) was stirred at room temperature under a nitrogen atmosphere for 30 minutes. The reaction mixture was then washed with 0.1 N HCl (80 mL), water (40 20 mL), and brine (40 mL), then dried over MgS04 and concentrated to a brown oil. The oil was stored at 0° C for 72 hours. The crude product was purified by flash chromatography on EM silica gel 60 (5 x 15 cm) eluting with 4:1 hexane-ethyl acetate (200 mL) followed by 2:1 hexane-ethyl acetate (1000 mL). The product-containing fractions were 25 combined and concentrated to give a light brown solid (2.3 g). The product was further purified by recrystallization from 15:1 hexane-ethyl acetate (80 mL) to give the title compound (1.48 g) as large white needles. The mother liquor was concentrated and washed with cold hexane (10 mL) to provide additional title compound (0.50 g) as 30 smaller white needles.
1H NMR (CDC13) d 0.73 (q, SiCH2CH3), 1.03 (t, SiCH2CH3), 5.26 (s, ArCH2), 6.92 (d, ArH-7), 7.53 (dd, ArH-6), 7.59 (d, ArH-5), 7.93 and 7.96 (two d's, ArH-2 and ArH-3) WO 99/20627 _56- PCT/US98/22110 SYNTHESIS OF ALLYL (1S.5R,6S)-6-f(1R) (ALLYLOXYCARB O NYLOXY)-ETHYLI -1-METHYL-2- f 4 (~ODOMETHYL)-li8-NAPHTHOSULTAMYL-METHYLI
5 . CARBAPEN-EM-3-CARBOXYLATE
OTES
R
\ \
HN-~O O
DIAD, PPh3 n=auyi O

~OTES HBa ~N
~PPh3 ~

H
TfOH \ \
OH
R OJ
\ \ ~ 1 MsCI
2) Nal ~/N-~O~O

WO 99/20627 -57_ PCT/ITS98/22110 Step 1-Allvl t3-f 1(R)-(allyloxvcarbonyloxy)-ethyll-2-f 1(R)-methyl-2-oxo-3-f4-(2-triethylsilanyloxy-methyl)-1~8-nar~hthosultamyll-prop 2R.3S)-4-oxo-azetidin-1~r11-(tripthenylphosphoranylidene)-acetate A solution of allyl {3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-[3-hydroxy-1(R)-methyl-2-oxo-propyl]-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (2.76 g, 4.3 mmol), 4-(2-triethylsilanyloxy-methyl)-1,8-naphthosultam (1.38 g, 3.9 mmol), and triphenylphosphine (1.53 g, 5.9 mmol) in anhydrous THF (20 mL) was placed under a nitrogen atmosphere and cooled in an ice bath.
10 Diisopropyl azodicarboxylate (1.2 mL, 5.9 mmol) was added over one minute. The resulting orange solution was stirred at 0° C for 30 minutes and then an additional 30 minutes at room temperature, after which the reaction mixture wad partitioned between ethyl acetate (80 mL) and brine (80 mL). The organic phase then dried over 15 MgS04 and concentrated. The concentrated solution was stored at 0°
C for 17 hours. The crude product was purified by flash chromatography on EM silica gel 60 (5 x 15 cm), eluting with 7:3 hexane-ethyl acetate (200 mL) followed by 1:1 hexane-ethyl acetate ( 1000 mL). The product-containing fractions were combined and 20 concentrated to yield the title compound (3.2 g) as an. off white foam.
Step 2: All lY (1S,5R,6S)-6-f(1R)-(allvloxvcarbonyloxy)-ethvll-1-methyl-2-f4-(2-trieth ls~ iianyloxv-meth 1~,8-naphthosultamyl-met~yll-carbapen-2-em-3-carbox- lv ate 25 A solution of allyl (3-[1(R)-(allyloxycarbonyloxy)-ethyl]-2-{1(R)-methyl-2-oxo-3-[4-(2-triethylsilanyloxy-methyl)-1,8-naphthosultamyl]-propyl}-(2R,3S)-4-oxo-azetidin-1-yl}-(triphenylphosphoranylidene)-acetate (3.2 g, 3.3 mmol) in anhydrous toluene (40 mL) was placed under a nitrogen atmoshere and heated 30 at reflux for 2.75 hours. The reaction mixture was allowed to cool to ~40° C and then concentrated to an orange oil. The oil was dissolved in dichloromethane (3 mL) and then stored at 0° C for 17 hours. The crude product was purified by flash chromatography on EM silica gel 60 (5 x 15 cm) eluting with 4:1 hexane-ethyl acetate (400 mL) followed 35 by 2:1 hexane-ethyl acetate (850 mL). The product-containing fractions were concentrated to give the title compound (1.6 g) as an off white foam.
1H NMR (CDC13) d 0.73 (q, SiCH2CH3), 1.02 (t, SiCH2C~g), 1.31 (d, 1-CH3), 1.45 (d, C I~CHO), 3.40 (dq, H-1), 3.44 (dd, H-6), 4.16 (dd, H-5), 4.59 (m, OC02CH2), 4.67 (d, 2-CHaHb), 4.88 (m, C02CH2), 5.13 (dq, CH3CH0), 5.26 (m, ArCH20 and 1 vinyl-H), 5.32-5.36 (m, 2 vinyl-H's}, 5.40 (d, CHa~, 5.50-5.54 (m, vinyl-H), 5.86-5.96 (m, vinyl-H), 6.00-6.10 (m, vinyl-H), 6.72 (dd, Ar H-7), 7.52 (m, Ar H5 and Ar H6), 7.94 and 7.98 (two d's, Ar H2 and Ar H3) Stgp 3' Allvl (1S,5R,6S)-6-f(1R)-(all~ycarbonyloxv)-ethyll-2-f4-(hvdroxvmethyl)-1.8-naphthosultamvl-methvll-1-methyl-carba ep n-2-em-3-carbox, 1 A solution of allyl (1S,5R,6S)-6-[(1R)-15 (allyloxycarbonyloxy)-ethyl]-1-methyl-2-[4-(2-triethylsilanyloxy-methyl)-1,8-naphthosultamyl-methyl]-carbapen-2-em-3-carboxylate (1.50 g, 2.15 mmol) in tetrahydrofuran (32 mL) was diluted with water (16 mL) and treated with 1N aqueous triflouromethanesulfonic acid (301 mL, 0.301 mmol). After stirring at room temperature for 20 20 minutes the reaction mixture was partitioned between dichloromethane (200 mL) and 5% aqueous bicarbonate (60 mL). The aqueous phase was extracted with dichloromethane (30 mL) and the combined organic phase was concentrated to yield the title compound (1.56 g) as a white foam.
Sten 4: Allvl (1S.5R.6S)-6-f(1R)-(allvloxvcarbonvloxv)-ethvll-2-f4-(iodomethvl)-1.8-nanhthosultamvl-methvll-1-methyl-carbauen-2-em-3-carbox~rlate Allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-2-[4-30 (hydroxymethyl)-1,8-naphthosultamyl-methyl]-1-methyl-carbapen-2-em-3-carboxylate was dissolved in dichloromethane (49 mL) and cooled in an ice bath under an atmosphere of nitrogen.
Triethylamine (0.524 mL, 3.8 mmol) followed by methanesulfonyl chloride (0.258 mL, 3.23 mmol) was added and the reaction mixture was allowed to stir at 2° C for 40 minutes. The reaction mixture was then partitioned between dichloromethane (250 mL) and 0.1 N

WO 99!20627 -59- PCT/US98/22110 aqueous HCl (200 mL), and the organic phase dried over MgS04 then concentrated and dried under vacuum for 20 hours. The resulting mesylate (1.7 g, quant.) was dissolved in acetone (60 mL) and sodium iodide (1.95 g, 13 mmol) was added. This suspension was stirred for 5 80 minutes at room temperature under a nitrogen atmosphere. The reaction mixture was then partitioned between dichloromethane (200 mL) and water (200 mL). The aqueous phase was extracted with dichloromethane (50 mL) and the combined organic phase was washed with 5% NaHS03 (2 x 75 mL), dried over MgS04 and then 10 concentrated. The resulting yellow oil was lyophilized from benzene (40 mL) to afford the title compound (1.5 g, quant.) as an amorphous yellow solid.
1H NMR (CDC13) 1.28 (d, 1-CH3), 1.42(d, CH_gCHO), 3.36 (dq, H-1), 3.42 (dd, H-6), 4.13 (dd, H-5), 4.42-4.68 (m, OC02CH2), 4.63 15 (d, 2-CHaHb), 4.76-4.94 (m, C02CH2), 5.10 (dq, CH~CHO), 5.20-5.34 (m, 3 vinyl-H), 5.38 (d, ArCH2I), 5.38 (d, CHaH~, 5.49 (m, vinyl-H), 5.80-5.90 (m, vinyl-H), 5.94-6.06 (m, vinyl-H), 6.72 (dd, Ar H-7), 7.60-7.64 (m, Ar H5 and Ar H6), 7.77 and 7.84 (two d's, Ar H2 and Ar H3) SYNTHESIS OF 3-AZIDO-1(I,4-DIMETHYL-PIPERAZINIUM) I?ROPANE TRIFLUOROMETHANESULFONATE
NaN3; Li CI, DMF N3 N~ N
CI~Br Cl~n 50°, 5 hrs Nal, 0.1 eq., CH3CN, 50°, 6 hr.
OTf-N
'~ ~ N AgOTf, CH3CN - N N+~n s n = 1, 2,3 and 4 Step 1: 1-Azido-3-chloronropane 25 Sodium azide (1.43 g, 22 mmol) and lithium chloride (0.93 g, 22 mmol) are added to DMF (10 ml) under nitrogen. To this was added 1,3-bromochloropropane (3.14g, 2.0 ml, 20 mmol) and the reaction mixture is stirred and heated at 50° far 5 hours. The reaction WO 99/20627 ~- PCT/US98/22110 mixture was diluted with water and extracted with petroleum ether.
The organic phase was washed twice with water, dried over MgS04 and evaporated to give 1,3-azidochloropropane.
Sten 2: 3-Azido-1(1.4-dimethyl-pinerazinium)-propane trifluoromethanesulfonate.
The azidochloropropane from Step 1 is dissolved in acetonitrile (8 ml) and treated with 1,4-dimethylpiperazine (1.12 g, 10 mmol). Sodium iodide (0.15 g, 1 mmol) is added and the reaction is 10 stirred under nitrogen at 50° for 6 hr. The solvent is removed under reduced pressure and the residue is triturated with ether, the ether solution is decanted off and the residue is taken up in acetonitrile (10 ml) and treated with silver trifluoromethane sulfonate (2.56 g, 10 mmol). After 10 min. the precipitated silver salts are filtered through a bed of celite and washed with a little acetonitrile. The filtrate and washings are evaporated to give the crude product which is crystallized from EtOH/Et20 to give the product.
Following the above procedure and starting with the 20 appropriate chlorobromoalkane one obtains the following compounds of Preparative Examples 8 to 10.

2-AZIDO-1(1,4-DIMETHYL-PIPERAZINITs7M)-ETHANE
TRIFLUOROMETHANESULFO~TATE.
OTf ' N N+~' N3 V \

4-AZIDO-1(1,4-DIMETHYL-PIPERAZINIUM)-BUTANE
TRIFLUOROMETHANESULFONATE.
OTf-'N N+~'N3 ~\

WO 99PZ0627 -sl- PCT/US98/2Z110 ~AZIDO-1(1,4-DIMETHYL-PIPERAZINIUM)-PENTANE
TRIFLUOROMETHANESULFONATE.
OTf--N N+'~ Na '-3-AZIDO-1(1.4-DIMETHYL-PIPERAZINIUM)-BUTANE
TRIFLUOROMETHANESULFONATE.
'N~ ' ,_~ B~ NaN3,Li CI, DMF
Br\~'Br -N N+ Br CH3CN, 65°, 2 hr. ~ ~ 50°, overnight OT
X
-N N+ N3 AgOTf, CH3CN N~ N ~ N3 Step 1: 3-Bromo-1(1,4-dimeth~-piperazinium)-butane bromide.
1,3-Dibromobutane (2.15 g, 1.2 ml, 10 mmol) is dissolved in acetonitrile (2 ml). DABCO (1.23 g, 11 mmol) is added. The reaction mixture is heated at 65° under nitrogen for 2 hr. The acetonitrile is removed under reduced pressure to give the product.
Steu 2: 3-Azido-1(1,4-dimeth~l-piperazinium)-)-butane trifluororaethanesulfonate, The product from Step 1 is dissolved in DMF (5 ml).
20 Sodium azide {0.71 g, 11 mmol) and lithium chloride (0.5 g, 11 mmol) are added followed by sodium iodide (0.149 g, 1 mmol). The reaction is stirred at 50°, under nitrogen overnight. The DMF is removed under reduced pressure with an external bath temperature of 40°. The residue is taken up in acetonitrile (5 ml) and methanol (5 ml) and 25 treated with silver triflate (2.8 g, 11 mmol). After 0.5 hr the precipitated silver salts are filtered off through a bed of celite and WO 99/20627 ~2- PCT/US98/2Z110 washed with a little methanol. The filtrate and washings are evaporated to give an oil. Trituration with ether gives the product.

5 N-(4-AZIDOMETHYLPHENYL)-1-( 1,4-DIMETHYL-PI~ERAZINIUM)ACETAMIDE TRIFLATE
C~I p MsCI/Et3N
H2N / \ CHzOH Py~H2Cl2 C 0 \ / H20H CH2CIz/T'HF C O \ / H20Ms NaN3 DMF
-N~/N~~~ \ / CH2N3 ' ~ C ~ \ / H2N3 2. AgOTf MeOH
Sten 1: N-(4-H.ydroxymethylphen~)chloroacetamide A solution of 4-aminobenzyl alcohol (2 g, 16.3 mmol), 10 pyridine (1.3 mL, 16.3 mmol) in CH2Clz (50 mL) is treated with chloroacetochloride (1.29 mL, 16.2 mmol) at 0°C. The mixture is stirred for 0.5 hr and then washed with water, 1N HCI, water and brine. It is dried over Na2S04 and concentrated. The residue is chromatographed over silica gel (eluent: hexane:ethyl acetate v/v 2:1) 15 to give the product {0.54 g) as a white solid.
Step 2: 4-(1-Chloroacetamido)benzvl methanesolfonate A solution of N-(4-hydroxymethylphenyl)chloroacetamide (0.25 g, 1.25 mmol), Et3N (0.5 20 mL, 3.46 mmol), solvent CH2C12 (30 mL) and THF (10 mL) is treated with methanesulfonyl chloride (0.1 mL, 1.29 mmol) at 0°C. The mixture is kept at 0°C for 0.5 hr and more CH2C12 is added. It is then washed with water and brine, dried over Na2S04 and concentrated to give the product (0.313 g).

Step 3: N-(4-Azidometh~phen ly )chlQroacetamide A mixture of 4-(1-chloroacetamido)benzyl methanesolfonate (0.313 g, 1.13 mmol), sodium azide (73 mg, 1.13 mmol), lithium chloride (48 mg, 1.13 mmol), DMF (10 mL) and 5 catalytic amount of sodium iodide is stirred at room temperature for 3 days. Most of the DMF is then removed under vacuum. The residue is taken up in ethyl acetate and washed with water 3 times. The organic layer is dried over Na2SOq and concentrated. The residue is chromatographed over silica gel (eluent: Hexane:ethyl acetate v/v 2:1) to give the product (77 mg) as a white solid.
Step 4: N-(4-azidomethYl_phenvl)-1-(1,4-dimeth3rl-~iperazinium)acetamide triflate A solution of N-(4-azidomethylphenyl)chloroacetamide 15 (77 mg, 0.343 mmol), 1,4-dimethylpiperazine (35 mg, 0.313 mmol) and CH3CN (3 mL) is stirred at room temperature for 3 days. The Solent is removed and the residue is dried under vacuum to give the product (0.116 g) as chloride. This chloride is dissolved in MeOH (3 mL) and treated with a solution of AgOTf (80 mg, 0.31 mmol) in CH3CN (0.5 20 mL). The mixture is stirred for 0.5 hr, then filtered to remove the white precipitate. The filter cake is washed with CH3CN. The combined filtrate is concentrated to give the product as a triflate salt.

25 N (4-(2-(ALLYLOXYCARBONYLAMINO)ETHYL)PHENYL)-1-(1,4-DIMETHYL-PIPERAZINIUM)ACETAMIDE TRIFLATE
p~ H ~ CI
NH2 ~ Cr v H2 \ / CH2CI2 H2 \ / ---O Et3N/CH2CI2 H ~ H
CI~~ / \ CH3CN ~ + / \ H
2. AgOTf O MeOH O
-OTf WO 99/20627 -~ PCT/US98l22110 Step 1:N-(4-aminophenethyl)-O-ally 1 carbamate To a solution of 4-aminophenethyl amine (2 g, 14.7 mmol) in CHZCl2 (20 mL) is added allyl chloroformate (0.533 mL, 4.89 mmol) at 0°C. The mixture is kept at 0°C for 0.5 hr and more 5 is added. It is then washed with 5% NaHC03, water and brine, dried over Na2S04 and concentrated. The residue is chromatographed over silica gel (eluent: ethyl acetate:hexane v/v 1:1) to give the product (1.38 g) as a white solid.
Step 2: N-(4-(2-(Allyloxycarbonylamino)ethyl)phenyl)chloroacetamide A solution of N-(4-aminophenethyl)-O-allyl carbamate (1.38 g, 6.28 mmol), Et3N (1.5 mL, 10.4 mmol) in CHZC12 (30 mL) is treated with chloroacetochloride (0.52 mL, 6.53 mmol) at 0°C. The mixture is stirred for 20 min and then washed with water, IN HCl, water and brine. It is dried over Na2S04 and concentrated to give the product (1.58) as a white solid.
Step 3N-(4-(2-(Allvi_oxvcarbonvlamino)ethyl)phenyl)-1-(134-dimeth,~~l-piperazinium)acetamide triflate A solution of N-(4-(2-(allyloxycarbonylamino)ethyl)phenyl)chloroacetamide (0.5 g, 1.68 mmol), 1,4-dimethylpiperazine (0.18 g, 1.61 mmol) and CH9CN (10 mL) is stirred at room temperature overnight. The solid is collected by filtration and dried under vacuum to give the white crystal product as chloride.
This chloride is dissolved in MeOH (10 mL) and treated with a solution of AgOTf (0.31 g, 1.21 mmol) in CH3CN (2 mL). The mixture is stirred for 0.5 hr, then filtered to remove the white precipitate. The filter cake is washed with CH3CN. The combined filtrate is concentrated to give the product as a triflate salt.

WO 99/20627 ~5- PCT/US98/22110 N-(2-ALLYLOXYCARBONYLAMINO)ETHYL)-1-(1 4-DIMETH '~L
PIPER,AZINIUM)ACETAMI~7E TRIFLATE
~ 0 H2N~NH2 C~~ ~N ~ C~~ H O
H20/E1~OH, H2N ~ ~ Et3~~2 CI~~H~C
O
pH-3.8 -f~N

2. AgOTf MeOH
_ r-. ~~.NJIo~
H
- -Off step 1: N-(2-aminoethyl)-O-allyl carbamate Ethelene diamine ( 1.8 g, 30 mmol) is dissolved in water (5.8 mL) with 5 drops of bromocresol green indicator.
Methanesulfonyl chloride (c= 0.92 g/ mL H20) is added until the solution turned to pale yellow (pH~3.8). The solution is diluted with IO EtOH (16 mL), vigorously stirred, and treated simutaneously with solutions of allylchloroformate (3.61 g, 30 mmol) in dimethoxyethane (5.8 mL) and potassium acetate (c=50% w/v H20) by alternate dropwise additions to maintain the pale yellow-green colouration of the indicator. After the additions are complete the mixture is stirred 15 for 1 hr and the volatiles are removed under vacuum. The residue is shaken with wetter and filtered to remove small quantities of the bis-derivative. The filtrate is washed with benzene, basified with excess 40% aqueous NaOH, and then extracted with benzene. The organic layer is washed with brine, dried over Na2S04 and concentrated to 20 give the product (0.6 g) as a yellow oil.
Step 2~ N-(2-Allvloxvcarbonylaminoethvl)chloroacetamide A solution of N (2-aminoethyl)-O-allyl carbamate (0.6 g,4.17 mmol), Et3N (1.0 mL, 6.93 mmol) in CHZC12 (20 mL) is treated 25 with chloroacetochloride (0.35 mL, 4.17 mmol) at 0°C. The mixture is stirred for 20 min and then washed with water, 1NHC1, water and brine. It is dried over Na2S04 and concentrated to give the product (0.474 g) as a black solid.
Step 3N-(2-AllyloxYcarbonylamino)ethyl)-1-(14- imeth3il-~perazinium)acetamide triflate A solution of N-(2-allyloxycarbonylaminoethyl)chloroacetamide (0.47 g, 2.13 mmol), 1,4-dimethylpiperazine (0.167 g, 1.5 mmol) and CH3CN (20 mL) is stirred 10 at room temperature for 3 days. The solvent is removed and the residue is dried under vacuum to give the product as the chloride.
This chloride is dissolved in MeOH (10 mL) and treated with a solution of AgOTf (0.38 g, 1.48 mmol) in CH3CN (2 mL). The mixture is stirred for 0.5 hr, then filtered to remove the precipitate. The filter cake is washed with CH3CN. The combined filtrate is concentrated to give the product as a triflate salt.

N-((3-ALLYLOXYCARBONYLAMINO)PROPYL)-1-(1,4-DIMETHYL-PIPERAZINIUM)ACETAMIDE TRIFLATE
Following the procedure described in Preparative Example 14, step 1 to step 3 and starting from 1,3-propanediamine, the title compound is obtained.

WO 99/Z0627 ~?- PCT/US98lZ2110 1V-(3-(2-ALLYLOXYCARBONYLAMINO)ETHYL)PHENYL)-1-(1,4 DIMETHYL-PIPERAZINIUM)ACETAMIDE TRIFLATE
02N I ~ 1. 02N ~ N3 1. Ph3P 02N ~ NH2 CI~~
H MsCI I ~

2. ~ 2. NaOH(2M) NaN3 HCI(10%) DMF

~I
02N w I N~ S~H 2N i I N~ CI ~'O
O EtOH ~ O
Et3N/CH~CI2 CI~~ ~ I N~ CH N ~ +~N i N
O ~ O ~ O
2. AgOTf MeOH oTr Step 1: 3-NitrQ,phene~;hyl azide To a solution of 3-nitrophenethyl alcohol (2 g, 11.9?
mmol), Et3N (3.0 mL, 20.8 mmol) in CHZCl2 (40 mL) is added methanesulfonyl chloride (1.0 mL, 12.9 mmol) at 0°C. After stirring for 0.5 hr, more CHzCl2 is added. The solution is washed with water 10 and brine, dried over Na2S04 and concentrated. The residue is dissolved in DMF (30 mL), sodium azide (0.8 g,12.3 mmol), lithium chloride {0.53 g, 12.5 mmol) and catalytic amount of sodium iodide are added. The mixture is stirred at room temperature overnight.
Most of DMF is then removed under vacuum and the residue is 15 washed with water and brine, dried over Na2S04 and concentrated.
The residue is chromatographed over silica gel (eluent: hexane:ethyl acetate v/v 4:1) to give a pale yellow oil (1.8 g) as desired product.
Step 2: 3-Nitro henet ylamine 20 A mixture of 3-nitrophenethyl azide (0.9 g, 4.69 mmol), triphenyl phosphine (1.22 g, 4.66 mmol) and CH2C12 (80 mL) is stirred at room temperature for 3 days. Solvent is removed and the residue is taken up in EtOH (20 mL). The solution is heated to 70°C, added 2M
NaOH aqueous solution (10 mL) and stirred at room temperature for 25 2 hrs. A 10% HCl aqueous solution (20 mL) is then added and refluxed for 2 hrs. After the mixture cool to room temperature, it is WO 99/20627 -~_ PCT/US98/22110 washed with benzene, basified with 5N NaOH to pH~ 12, extracted with ethyl acetate. The organic layer is dried over Na2S04 and concentrated to give the product (0.63 g) as a yellow oil.
Step 3~ N (2-(3-Nitro~hen~~l)ethyl)-O-allyl carbamate To a solution of 3-nitrophenylamine (0.62 g, 3.73 mmol) and Et3N (0.8 mL, 5.54 mmol) in CH2C12 (20 mL) is added allyl chloroformate (0.4 mL, 3.77 mmol) at 0°C. The mixture is kept at 0°C
for 0.5 hr and more CHZC12 is added. It is then washed with 5%
10 NaHC03, water and brine, dried over Na2S04 and concentrated to give the product (0.9 g).
Step 4: N-(2-(3-Aminophen lv )ethyl)-O-allvl carbamate A mixture of N-(2-(3-Aminophenyl)ethyl)-O-allyl carbamate (0.9 g, 3.6 mmol), tin{II) chloride dehydrate (4.1 g, 18 mmol) in EtOH (50 mL) is heated at 70°C for 1 hr. The EtOH is removed and the residue is added water, basified with 5N NaOH to pH>10, extrated with CHzCl2. The organic layer is washed with water and brine, dried over Na2S04, concerntrated to give the product (0.66 g).
Sten 5: N-((3-(2-all ~loxycarbonvlamino)eth,~rl).phenvl)chloyoacetamide A solution of N-(2-(3-Aminophenyl)ethyl)-O-allyl carbamate (0.66 g,3.0 mmol), Et3N (0.65 mL, 4.5 mmol) in CH2C12 (20 25 mL) is treated with chloroacetochloride (0.26 mL, 3.2 mmol) at 0°C.
The mixture is stirred for 20 min and then washed with water, lNHCI, water and brine. It is dried over Na2S04 and concentrated to give the product (0.62 g).
ten 6' N-(3-(2-Al~,vloxycarbonvlamino)ethyl), .~henvl)-1-( la4-dimeth,~l-~iperazinium)acetamide triflate A solution of N-((3-(2-allyloxycarbonylamino)ethyl)phenyl)chloroacetamide (0.32 g, 1.08 mmol), 1,4-dimethylpiperazine (0.121 g, 1.08 mmol) and CH3CN (10 mL) is stirred at room temperature overnight. The solvent is removed WO 99/20627 ~9- PCT/US98~22110 and the residue is dried under vacuum to give the product as chloride. This chloride is dissolved in MeOH ( 10 mL) and treated with a solution of AgOTf (0.27 g, 1.08 mmol) in CH3CN (1 mL). The mixture is stirred for 0.5 hr, then filtered to remove the white 5 precipitate. The filter cake is washed with CH3CN. The combined filtrate is concentrated to give the product as a triflate salt.

N-( 2-( 2-ALLYLOXYCARB ONYLAMINO )ETHYL) PHENYL)-1-( 1 4 IO DIMETHYL-PIPERAZINIUM)ACETAMIDE TRIFLATE
Following the procedure described in Example 16, step 1 to step 6 starting from 2-nitrophenethyl alcohol, the title compound is obtained.

(S)-f )- 3-AZIDO-1-(1,4-DIMETHYL-PIPERAZINIUM)-2-METHYLPROPANE TRIFLATE
~. rt2o/2,6-lutidine NaN3 CH2CI2 ~H --~ N3~OH N3~N+ N-DMF 2. ~ ~ U
-(~N-CH3CN -OTf ~p 1: fR)-< )-3-azido-2-meth,~propanol 20 A mixture of (R)-()-3-bromo-2-methylpropanol (1 g, 6.53 mmol), sodium azide (0.47 g, 7.23 mmol), lithium chloride (0.31 g, 7.29 mmol) and DMF (10 mL) is heated at 50°C overnight. Most of DMF is then removed. The residue is taken up in ethyl acetate, washed with water and brine, dried over Na2S04 and concentrated to 25 give the product (0.64 g).
step 2~ fS)-< )- 3-Azido-1-(1 4-dimethvl-ninerazinium)-2-methylpropane triflate (R)-( )-3-azido-2-methylpropanol (0.64 g, 5.56 mmol) is 30 dissolved in CHzCl2 (40 mL). 2,6-lutidine (1.9 mL, 16.3 mmol) is added at -20°C followed by adding trifalic anhydride (1.4 mL, 8.32 mmol).
The mixture is kept in -20°C for 40 min, diluted with CHzCl2, washed with water, 0.1 N HCl aqueous solution, water and brine, dried over NazS04 and concentrated to give the trifalte (1.33 g). The triflate (0.7 g, 5 2.83 mmol) is reacted with 1,4-dimethylpiperazine (0.31 g, 2.77 mmol) in CH3CN for 3 hrs. The solvent is removed and the residue is dried under vacuum to give the product.

10 ~1~, 5R 6S)- -2-t6-[2-(4-(3-AMMONIOPROPYL)- 14-DIMETHYL-P~PERAZIN-1-IO)-ETHYLI-1,1-DIOXO-2-H-1-THIA-2-AZA
ACENAPHTHALEN-2-YL-METHYLI-6=[1(R)-HYDROXYETHYLI-1 OTf OTf-CH2=CH-CH20C00 ~ -N~ ~ N
H H N, ~ ~2 CH3CN, rt, 1 hr, conc. soln COOCH2-CH=CH2 2 OTf-N ~ ~ N3 CHp=CH-CH20C00 ~ ~ ~ 1) DMF,[ (CgH5)3F14Pdo~
H H N- - CsH5SiH3, rt, 10 min.
2) H2, 5% Rh/C, 1.5 hr, 1 atm.
O N
COOCH2-CH=CH2 \N N ~ NH3+

H H
N
~O
COO-15 ~S~g~yl (1S,5R 6S)--6-[(1R)-(allylox~carbon~xy)-ethvll ~ (6 [2 (4 (3-azidop,~o_gvl)- 14-dimethyl~iperazin-1-iol-eth'~ ~ - ioxo-2-H-1-WO 99/20627 -71_ PC"T/US98/Z2110 thia-2-aza-acenaphthalen-2-~1-methvll-1-methyl-carbapen-2-em-3-carboxylate bis (trifluoromethanesulfonate).
Allyl (1S,5R,6S)--6-[(1R)-(allyloxycarbonyloxy)-ethyl]-2-[6-(2-trifluoromethanesulfonyloxyethyl)-ethyl]- 1,1-dioxo-2H-1-thia-2-5 aza-acenaphthalen-2-yl-methyl}-1-methyl-carbapen-2-em-3-carboxylate from Step 4, Preparative Example 2, (150 mg,) is dissolved in acetonitrile (1.5 ml), 3-Azido-1(1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate (85 mg,) from Step 2, Preparative Example 7, is added. The reaction is stirred 5 min. and the solvent is 10 evaporated under vacuum to give a thick oil which is stirred at room temperature for 45 min. The residual solvent is removed to give the product which is used in the next step without purification.
StP 2: (1 ,5R 6S)- -2-t6-f2-(4-(3-ammoniopropvl)- 1,4-dimethyl-15 piperazin-1-io)-ethyll-11-dioxo-2-H-1-thia-2-aza-acenanhthalen-2-yl-meth,-6-f 1(R)-hydroxvethyll-1-methyl-carbapen-2-em-3-carboxylate dichloride.
Allyl (1S,5R,6S)--6-[(1R)-(allyloxycarbonyloxy)-ethyl]- 2-(6-[2-(4-(3-ammoniopropyl)-1,4-dimethyl-piperazinium]-ethyl-1,1-dioxo-20 2H-1-thia-2-aza-acenaphthalen-2-yl-methyl}-1-methyl-carbapen-2-em-3-carboxylate bis (trifluoromethanesulfonate) from Step 1 is dissolved in DMF (1.5 ml), Phenylsilane ( 115m1) is added, followed by tetrakis(triphenylphosphine)palladium(0) (22.5 mg, 0.019 mmol). The reaction is stirred at room temperature for 10 min. then diluted with 25 ether (5 ml) to precipitate the product. The ether solution is decanted off and the residue washed with 2x7 ml ether. The combined ether solutions are centrifuged and the precipitate combined with the original precipitate is dissolved in acetonitrile/water 1/1 (2 ml). This solution is applied to a column of Macro-Prep CM weak cation 30 exchange resin (5 ml). The column is eluted with 1:1 acetonitrile-water (8 ml) followed by water ( 10 ml). The resin is eluted with 5%
aqueous sodium chloride (75 ml) and collected in 5x 15 ml fractions.
The first four fractions containing the product are combined and treated with 5% rhodium on carbon (50 mg) and stirred under an 35 atmosphere of hydrogen. After 1 hour the catalyst is filtered off and the filtrate is loaded onto a Rainin Microsorb C18 RP HPLC column (2x 30 cm). Gradient elution with acetonitrile/0.12M aqueous ammonium chloride gives pure product containing fractions which are combined and concentrated under vacuum. The concentrated product is applied to a column of Amberchrome CG-161 4 ml) which 5 is eluted with water (10 ml) followed by 1:1 acetonitrile-water (10 ml).
The acetonitrile-water eluate is lyophilized to give the product.

(1S.5R,6S)- -2-f6-f2-(4-(2-AMMONIOETHYL)- 1,4-DIMETHYL-10 PIPERAZIN-1-IO)-ETHYLI-1,1-DIOXO-2-H-1-THIA-2-AZA-ACENAPHTHALEN-2-YL-METHYL)-6-~1(R)-HYDROXYETHYLI-1-METHYL-CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE.
N ~ +-~NH3+

OH
H H N, ~ --N
O
COO-15 Substituting 2-Azido-1(1,4-dimethyl-piperazinium)-ethane trifluoromethanesulfonate for 3-Azido-1(1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate in Example 1, Step 1 above, and following the procedure of Step 2 one obtains the desired product.

(1SZ5R.6S)-2-(6-(2~4-(4-AMMONIOBUTYL)- 1.4-DIMETHYL-PIPERAZIN-1-IO)-ETHYLI-1.1-DIOXO-2-H-1-THIA-2-AZA-ACENAPHTHALEN-2-YL-METHYLI-6-f 1(R)-HYDROXYETHYLI-1-25 METHYL-CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE.

N V \-~NH3+

OH
H H N.O
N
~(O
COO-Substituting 4-Azido-1(1,4-dimethyl-piperazinium)-butane trifluoromethanesulfonate for 3-Azido-1( 1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate in Example 1, 5 Step 1 above, and following the procedure of Step 2 one obtains the desired product.

(1S 5R,6S)- -2-(6-f2-(4-(5-AMMONIOPENTYL)- 1,4-DIMETHYL-10 PIPERAZIN-1-IO)-ETHYLI-1,1-DIOXO-2-H-1-THIA-2-AZA-ACENAPHTHALEN-2-YL-METHYL)-6-f 1(R)-HYDROXYETHYLI-1-METHYL-CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE.
~n N U ~~\'~ NH3+
~ 2CI-OH
H H N~~ ' O N~ 2 COO-Substituting 5-Azido-1(1,4-dimethyl-piperazinium)-15 pentane trifluoromethanesulfonate for 3-Azido-1(1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate in Example 1, Step 1 above, and following the procedure of Step 2, one obtains the desired product.

WO 99/20627 -7~ PCT/US98/22110 (1S 5R 6S)- -2-f6-f2-(4-(3-AMMONIO-3-METHYLPROPYL)- 14 DIMETHYL-PIPERAZIN-1-IO)-ETHYLI-1 1-DIOXO-2-H-1-THIA-2 AZA-ACENAPHTHALEN-2-YL-METHYLI-6-f 1(R)-CARBOXYLATE DICHLORIDE.
N ~ ~ NH3+
OH ~ ~ ~ 2 CI-H H N~ S

O
COO-Substituting 3-Azido-1(1,4-dimethyl-piperazinium)-butane trifluoromethanesulfonate for 3-Azido-1(1,4-dimethyl-10 piperazinium)-propane trifluoromethanesulfonate in Example 1, Step 1 above, and following the procedure of Step 2, one obtains the desired product.

15 (1S,5R~6S)-6-(1(R)-HYDROXY-ETHYL)-1-METHYL-2-f 6-(2-(4-(3(R)-AMO,~IO-2-METHYLPROPYL)- 1,4-DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1.1-DIOXO-1H-1-THIA-2-AZA-ACENAPHTHYLEN-2-YL-Substituting 3-Azido-2-methyl-1(1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate for 3-Azido-i(1,4-dimethyl-piperazinium)-propane trifluoromethanesulfonate in Example 1, Step 1, and following the procedure of Step 2, one obtains the desired product.

(1S.5R.6S)- -2-(6-(2-(4-(3-METHYLAMMONIOPROPYL)- 14 DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1 1-DIOXO-2-H-1-THIA-2 AZA-ACENAPHTHALEN-2-YL-METHYL)-6-f 1(F)-CARBOXYLATE DICHLORIDE.

~N ~ Ni ~ NH2+
OH \ ~ \ 2CI-H H
N. V2 O N
COO-Starting with allyl N-[3-(1,4-dimethylpiperazinium)propyl)-N-methylcarbamate trifluoromethanesulfonate and the product of Preparative Example 2, and following the procedure of steps 1 and 2 of Example 11, one obtains the title compound.

(1S R.6S)-6-(1(R)-HYDROXY-ETHYL)-1-METHYL-2-(6-(2-(4-(3-AMONIOPROPYL)AMINOCARBONYLMETHYL)- 14-DIMETHYL
PIPERAZIN-1-IO)-ETHYL)-1 1-DIOX(~-1H-1-THIA-2-AZA
ACENAPHTHYLEN-2-YL-METHYL) CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE

OH H ~ \
N ~ N \
O O,~S. ~,~ /
O O- +
O CN J
2ci N+

NH
N+H3 Substituting the products of Preparative Examples 2 and 15, in step 1 of Example lI, followed by step 2 of Example 11, provides the title compound.

(1S R,6S)-6-(1(R)-HYDF~OXY-ETHYL)-1-METHYL-2-(6-(2-(4-(2-AMONIOETHYL)AMINOCARBONYLMETHYL)- 1,4-DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1,1-DIOXO-1H-1-THI -2-AZA-~CENAPHTHYLEN-2-YL-METHYLICARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE
OH H ~ \
N ~ N \
O O~~SO /.,.- N /
O O- +
2~~- C

NH

Substituting the product of Preparative Examples 2 and 14 in step 1 of Example 11 followed by Step 2 of Example 11 provides the title compound.

wo ~no62~ _77- Pcrivs9sn2>< ><o S~'~[THESIS OF (1S,~5R,6S)-2-(3-(2-f4-(3-AMMONIO-PROPYL)-1,4-DIMETHYL-PIPERAZIN-1-IOl-ETHYL?-1,8-NAPHTHOSULTAMYL-METHYL)-6-f(1R)-HYDROXY-ETHYLI-1-/ I \ OTf Tf~ N3 ROCO H H Me ~ /
_ \
Me N S,O ~NJ
O
O

R = ally) 1 ) Pd(0) deblock HO H H Me ~
/ N 2) H2, 5% Rh/C_ N- S, 3) purification Me . p O ~ Np N-S,. C ~ N
p O /N, O O CO~ 2 CQ
2 Tf~ ~ Ns ~H3 Step 1' All (1S,5R,6S)-6-f(1R)-(allyloxvcarbonylox~yll-2-(3-(2-f4-(3-azido-prop- lv )-11,~4-dimeth~l-~inerazin-1-ioi-ethvll-1 8-nanhthosultamvl-methyl)-1-methyl-carbapen-2-em-3-carboxlate bis(trifluoromethanesulfonate) A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-1-methyl-2-{3-[2-(trifluoromethanesulfonyloxy)-ethyl]-1,8-naphthosultamyl-methyl]-carbapen-2-em-3-carboxylate (0.141 mmol) in anhydrous acetonitrile (1.0 mL) is added to 1-(3-azido-propyl)-1,4-dimethylpiperazinium trifluoromethanesulfonate (51 mg, 0.144 mmol). The solution is concentrated under vacuum to approximately 0.2 mL and let stand at room temperature for 2 hours. The oil is triturated with diethyl 20 ether, the solvent decanted, and the residue dried under vacuum to afford the title compound as an amorphous solid. It is used in the next step without further purification.

WO 99/20627 _7~ PCT/US98/22110 Step 2: (1S R,6S)-2-(3-f2-f4-(3-Arnmonio-.~roR lv )-1,4-dimeth ~~1-piperazin-1-iol-ethvll-1,8-naphthosultamvl-methyl)-6-f ( 1R)-l~vdrox~
ethyl-1-methyl-carbapen-2-em-3-carboxXlate dichloride A solution of crude allyl (1S,5R,6S)-6-[(1R)-5 (allyloxycarbonyloxy)-ethyl]-2-(3-{2-[4-(3-azido-propyl)-1,4-dimethylpiperazin-1-io]-ethyl)-1,8-naphthosultamyl-methyl)-1-methyl-carbapen-2-em-3-carboxylate bis(trifluoromethanesulfonate) (0.141 mmol) in anhydrous dimethylformamide (1.4 mL) is treated with 5,5-dimethyl-1,3-cyclohexanedione (dimedone, 59 mg, 0.423 10 mmol), triphenylphosphine (5.5 mg, 0.021 mmol) and tetrakis(triphenylphosphine)palladium(0) (8.1 mg, 0.007 mmol). The mixture is placed under a nitrogen atmosphere, treated with N,N-diisopropylethylamine (0.074 mL, 0.423 mmol), and stirred at room temperature. After 20 minutes, the mixture is added to diethyl ether 15 (13 mL) and centrifuged. The supernatant is decanted from the orange colored solid which is washed with more ether (14 mL) and dried under vacuum to afford crude (1S,5R,6S)-2-(3-{2-[4-(3-azido-propyl)-1,4-dimethylpiperazin-1-io]-ethyl}-1,8-naphthosultamyl-methyl)-6-[( 1R)-hydroxy-ethyl]-1-methyl-carbapen-2-em-3-carboxylate 20 trifluromethanesulfonate.
The deallylated product is dissolved in tetrahydrofuran (2 mL), water (4 mL) and ethanol (3 mL), treated with 5% rhodium on carbon (17 mg), and stirred under an atmosphere of hydrogen. After 65 minutes, the mixture is filtered and the filtrate applied to a 25 column of Macro-Prep CM weak cation exchange resin (3 mL). The column is eluted with 1:1 acetonitrile-water (10 mL), water (12 mL), and 5% aqueous sodium chloride (40 mL). The saline fractions are concentrated under vacuum to 9 mL and loaded onto a Ranin Microsorb Clg RP HPLC column (2 x 30 cm) which is gradiently 30 eluted with acetonitrile in 0.12M aqueous ammonium chloride. The product containing fractions are concentrated under vacuum then applied to a column of Amberchrom CG-161 which is eluted with water (25 mL) followed by 20% isopropanol in water. The product eluted sharply with isopropanol-water. The product containing 35 fractions diluted with water, concentrated under vacuum to 4 mL, and lyophilized to afford the title compound.

SYNTHESIS OF (1S,5R 6S)-6-f(1R)-HYDROXY-ETI~~1-1-METHYL
2-(3-(2-f4-(3-METHYLAMMONIO-PROPYL)- 1,4-DIMETHYL
PIPERAZIN-1-IOl-ETHYLI-1,8-NAPHTHOSULTAMYL-METHYL)-/ I \ ~ Tf~ Me~N.C02R
ROCO H H Me \ / OTf N- S, Me " O --N
N~ O
O R = allyl R = ally!
/ \
\ HO Me \ I / Nd / N~ 1 ) Pd(0) deblock Me H H N -S ,. C
~N-S, C ~ 2) purification ~~ O O /~
''',,~ ~ O / N~ O 2 GQ
N ~ C02R CO~ NH
2 Tf~ ~ ~ 2 Me Me Sit .p 1' All lv (1S 5R,6S)-2-f3-(2-(4-l4-(al~il~cxvcarbonvll-4-aza-pent-1-, 1.4-dimeth ~~1-piperazin-1-io 1-ethyl)-1.8-naphthosultamyl-methyll-6-I(1R)-(allyloxycarbon~oxv)-ethyll-1-methvl-carbapen-2-em-3-carboxxlate bis(trifluoromethanesulfonate~
A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-1-methyl-2-{3-(3-15 (trifluoromethanesulfonyloxy)-propyl]-1,8-naphthosultamyl-methyl)-carbapen-2-em-3-carboxylate (0.172 mmol) in anhydrous acetonitrile (0.9 mL) is treated with allyl N-[3-(1,4-dimethylpiperazinium)-propyl]-N-methyl-carbamate trifluoromethanesulfonate (76 mg, 0.188 mmol).
The solution kept at room temperature for one hour then stored in a 20 freezer at -10°C for 3.5 days. The solvent is evaporated under vacuum. The residual oil is triturated with diethyl ether, the solvent decanted, and the residue dried under vacuum to afford the title WO 99/20627 Via- PCT/US98/22110 compound as an amorphous solid. It is used in the next step without further purification.
Step 2~ (1S 5R,.,.65)-6-f~lR)-Hydroxy-ethvll-1-methyl-2-(3-(2-f4-(3-5 methvlammonio-propyl)- 1,4-'methyl-Ri~erazin-1-iol-eth lv 1-_1,,8-naphthosultamvl-meth3rl)-carbapenem-2-em-3-carbo~vlate dichloride A solution of crude allyl (1S,5R,6S)-2-[3-(2-(4-[4-(allyloxycarbonyl]-4-aza-pent-1-yl]-1,4-dimethylpiperazin-1-io}-ethyl)-1,8-naphthosultamyl-methyl]-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-1-10 methyl-carbapen-2-em-3-carboxylate bis(trifluoromethanesulfonate) (0.172 mmol) and tetrakis(triphenylphosphine)palladium(0) (10.0 mg, 0.086 mmol) in anhydrous dimethylformamide (0.86 mL) is placed under a nitrogen atmosphere and treated with phenylsilane (0.191 mL, 1.55 mmol). After stirring for 20 minutes at room temperature, 15 the dark solution is added to diethyl ether (12 mL) to give a brown precipitate. The supernatant is decanted and the residue dried under vacuum to give a solid (246 mg).
The solid is dissolved in 1:1 acetonitrile-water (3 mL) and applied to a column of Macro-Prep CM weak cation exchange resin (4 20 mL). The column is eluted with 1:1 acetonitrile-water (7 mL), water (18 mL), 5% aqueous NaCI (9 mL), and 1:1 isopropanol-10% aqueous NaCl (10 mL). The product containing fractions (5% NaCI and 1:1 iPrOH-10% NaCI) wre combined and concentrated under vacuum to remove isopropanol. The resulting solution is applied to a column of 25 Amberchrom CG-161 resin (5 mL) which is eluted with water (20 mL) followed by 20% isopropanol in water (6 mL). The product containing fractions (20% iPrOH) are concentrated under vacuum, diluted with water, reconcentrated, filtered, and lyophilized to give a pale yellow, amorphous solid (43 mg, 35% yield). This material is further purified 30 by HPLC on a Ranin Microsorb Clg RP column (2 x 30 cm) which is gradiently eluted with acetonitrile (0-30%) in 0.12M aqueous ammonium chloride. The product containing fractions (30% MeCN
in 0.12M NH4Cl) are desalted on Amberchrom CG-161 (5 mL) as described above and the final aqueous solution lyophilized to provide 35 the title compound as an amorphous, white solid.

WO 99/Z0627 -$1- PCT/US98122110 (1S.5R.6S)-6-(I(R)-HYDROXY-ETHYL)-1-METHYL-2-(6-(2-(4-(4-AMONIOMETHYLPHENYL)AMINOCARBONYL)METHYL)-1,4-DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1 1-DIOXO-1H-1-THLA-2-AZA-ACENAPHTHYLEN-2-YL-METHYL)CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE
N / _.N \
O Q..S. ~ /
O O O ...- N+
C
O

H3+N
Stet 1: All lY (_1-S_,5R,6S)--6-f(1R)-(all loxycarbonylox~~)-eth~L 1-methvl-2-(6-(2-(4-(4-azidomethy~phenyl)aminocarbonvl)meth,~rl)- 1 4-dimeth,L
10 ~iperazin-1-io)-ethyl)-1,I-dioxo-1H-1-thia-2-aza-acenanhth lv en-2;y1-methvllcarbapen-2-em-3-carboxvlate bis-trifluormethylsulfonate.
Starting with N-(4-azidomethylphenyl)-1-(1,4-dimethylpiperazinium)acetamide triflate (1$.5 mg) from Preparative Example 12 and reacting it with the product of Step 4, Preparative 15 Example 2, according to the procedure of Example 1, step 1, one obtains the title product, which is used in the next step without further purification.
~gp 2. (1S,5R,6S)-6-(1(R)-hydroxv-ethyl)-1-metal-2-(6-(2-(4-(4-20 amoniomethylnhenvl)aminocarbon lv )meth ly )-1,4-dimethyl-piperazin-I-io)-ethyl)-1.1-dioxo-1H-1-thia-2-aza-acenaphth ly en-2 ;~~1-methyllcarba-pen-2-em-3-carboxvlate dichloride.
The product from Step 1 is dissolved in DMF (0.2 ml), Phenylsilane ( 30 ml) is added, followed by 25 tetrakis(triphenylphosphine)palladium(0) (2 mg). The reaction is stirred at room temperature for 15 min. then diluted with ether (2 ml) to precipitate the product. The ether solution is decanted off and the residue washed with 2x2 ml ether. The combined ether solutions are centrifuged and the precipitate combined with the original precipitate 5 is dissolved in acetonitrile/water 1/1 (1 ml). This solution is applied to a column of Macro-Prep CM weak cation exchange resin {1.5 ml).
The column is eluted with 1:1 acetonitrile-water (3 ml) followed by water (3 ml). The resin is eluted with 5% aqueous sodium chloride (6 ml) and collected in 1 ml fractions. The fractions containing the 10 product are combined and treated with 5% rhodium on carbon and stirred under an atmosphere of hydrogen. After 1.5 hour more catalyst is added and the reduction continued for 1 hr.The catalyst is filtered off and the filtrate is loaded onto a Rainin Microsorb C18 RP
HPLC column (2x 30 cm). Gradient elution with acetonitrile/0.12M
15 aqueous ammonium chloride gives pure product containing fractions which are combined and concentrated under vacuum. The concentrated product is applied to a column of Amberchrome CG-161 1.5 ml) which is eluted with water (5 ml) followed by 1:1 acetonitrile-water (5 ml). The acetonitrile-water eluate is lyophilized to give the 20 product.

WO 99/20627 ~3- PCT/US98l22110 ~1S.5R.6S)-6-(1(R)-HYDROXY-ETHYL)-1-METHYL-2-(6-(2-(4-(4-(2-AMONIOETHYL)PHENYL)AMINOCARBONYL)METHYL)-14 DIMETHYL-PIP RAZIN-1-IO)-ETHYL)-11-DIOXO-1H-1-THIA-2 5 AZA-ACENAPHTHYLEN-2-YL-METHYL)CARBAPEN-2-EM-3-CARBOXYLATE DICHLORIDE
Starting with the products of Preparative examples 2 and 13, and following the procedure of steps 1 and 2 of Example 11, one 10 obtains the title compound.

( 1S, 5R,6S)-6-( 1(R)-HYDROXY-ETHYL)-1-METHYL-2-(6-(2-(4-(2-(2-AMONIOETHYL)PHENYL)AMINOCARBONYL)METHYL)-1,4-15 DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1,1-DIOXO-1H-1-THIA-2-CARBOXYLATE DICHLORIDE
OH H . I \
N ~ N \
O 0 .S~ ~ ~ /
O O O N
C

,_ ..
NH
N+Hs~ /

WO 99/20627 ~- PCT/US98/22110 Starting with the product of Preparative examples 2 and 17, and following the procedure of steps 1 and 2 of Example 11, one obtains the title compound.

( 1S,5R,6S)-6-( 1(R)-HYDROXY-ETHYL)-1-METHYL-2-(6-(2-(4-(3-(2-AMONIOETHYL)PHENYL)AMINOCARBONYL) METHYL)- 1.4-DIMETHYL-PIPERAZIN-1-IO)-ETHYL)-1,1 METHYL)CARBAPEN-2-EM-3-CARBOXYLATE
DICHLORIDE
N / _N I \
O ~-S~ ~ /
O 00 O N+
C

H3+N~J~
Starting with the product of Preparative Examples 16 and 2, and following the procedure of steps 1 and 2 of Example 11, one obtains the title compound.

wo ~no62~ -85- rcT~s9sn2mo SYNTHESIS OF (lSa5R 6S)-2-f3-f2-(4-f f4-(2 AMMONIO ETHYL) PHENYLCARBAMOYLI-METHYL}-1~4-DIMETHYL PIPERAZIN 1 IO)-ETHYLI-1,8-NAPHTHOSULTAMYL-METHYLI-6-f(1R)-CARBOXYLATE DICHLORIDE
O / \ Tf~ H
f ROCO H H Me \ I / OTf ~~ N ~ N
Me N-S~ ~NJ O ~ NHC02R
O
N~ O R = ally) O

R = allyl / \
/ \ HO H ~ Me \ I / Nd \ I / N~ 1 ) Pd(o) deblocl~e N-Y' O
/N-~: ~ ~ 2) Purification N ~ O ~~ O
O O i Np O ~ 2 GQ
2 Tf~ ~O / NH
HN / \
~~ NHC02R H N
Step 1~ Allvl (1S 5R,6S)-2-f3-f2-(4-ff4-(2-allvloxvcar~onvla_mino ethyl) phenvlcarbamoyll-meth ly 1-1.4-dimethyl-piperazin-1-io)-eth. ly 1-1,8-IO nanhthosultamvl-methyl)-6-f(1R)-allyloxvcarbony~g~ -v eth lv 1~1-n~eth3~1-carbauenem-2-em-3-carboxylate bis(trifluoromethanesulfonate) A solution of allyl (1S,5R,6S)-6-[(1R)-(allyloxycarbonyloxy)-ethyl]-1-methyl-2-{3-[3-15 (trifluoromethanesulfonyloxy)-propyl]-1,8-naphthosultamyl-methyl}-carbapen-2-em-3-carboxylate (0.172 mmol) in anhydrous acetonitrile (0.9 mL) is treated with allyl N-(2-{4-[2-(1,4-dimethylpiperazinium)-acetylamino]-phenyl}-ethyl)-carbamate trifluoromethanesulfonate (98 mcr (1 1 RtZ mtrl~~l Thn onl"tin» lrn,~+ ..+ ..,"",., +llw~rv/~N.1+~~NIO ~n.~
..r.,.

WO 99/0627 ~~ PGT/US98/22110 ether, the solvent decanted, and the residue dried under vacuum to afford the title compound as a foam. It is used in the next step without further purification.
5 Step 2: (1S.5R.6S)-2-(3-f2-(4-(f4-(2-ammonio-eth~phenylcarbamovll-meth 1~_)-1,4-dimeth ~~1-piperazin-1-io)-ethyll-1,8-naphthosultam3rl-n~ethvll-6-((1R)-h3rdrox -v ethyll-1-methyl-carbapenem-2-em-3-carboxvlate dichloride A solution of crude allyl (1S,5R,6S)-2-{3-[2-(4-([4-(2-10 allyloxycarbonylamino-ethyl)-phenylcarbamoyl]-methyl}-1,4-dimethylpiperazin-1-io)-ethyl]-1,8-naphthosultamyl-methyl}-6-[(1R)-allyloxycarbonyloxy-ethyl]-1-methyl-carbapenem-2-em-3-carboxylate bis(trifluoromethanesulfonate) (0.172 mmol) and tetrakis(triphenylphosphine)palladium(0) (10.0 mg, 0.086 mmol) in 15 anhydrous dimethylformamide (0.86 mL) is placed under a nitrogen atmosphere and treated with phenylsiiane (0.191 mL, 1.55 mmol).
After stirring for 20 minutes at room temperature, the solution is added to diethyl ether (13 mL) to give a precipitate. The supernatant is decanted and the solid residue dried under vacuum.
20 The solid is dissolved in 1:1 acetonitrile-water (3 mL) with added tetrahydrofuran (4 mL) and water (0.5 mL) and applied to a column of Macro-Prep CM weak cation exchange resin (2.5 mL).
The column is eluted with 1:1 acetonitrile-water (7 mL), water (12 mL), and 1:1 isopropanol-10% aqueous NaCl (8 mL). The product 25 containing fractions (1:1 iPrOH-10% NaCI) are concentrated under vacuum to a hazy suspension and applied to a column of Amberchrom CG-161 resin (4 mL). The column is eluted with water (15 mL) followed by 20% isopropanol in water (6 mL). The product containing fractions (20% iPrOH) are concentrated under vacuum 30 and lyophilized to give the title compound.

Claims (12)

WHAT IS CLAIMED IS:

1. A compound represented by formula I:

as well as pharmaceutically acceptable salts thereof, wherein:
R1 represents H or methyl;
CO2M represents a carboxylic acid, a carboxylate anion, a pharmaceutically acceptable ester group or a carboxylic acid protected by a protecting group;
P represents hydrogen, hydroxyl, F or hydroxyl protected by a hydroxyl-protecting group;
A-Q-L-B represents a side chain wherein:
A is a C1-6 alkylene group, straight or branched, and optionally interrupted or terminated by 1-2 of -O-, -S-, NR a-, -C(O)- and -CH=CH-;
Q represents in which:
b is 2 or 3;
and X- is a charge balancing counterion;
L represents a C1-8 alkylene group, unsubstituted or substituted with 1-3 R c groups, and is interrupted or terminated by 1- Het is a heteroaryl group;
B represents 1) or -NR f R9 or wherein represents a 5-10 membered mono- or bicyclic, N-containing heteroaryl group, optionally containing 1-4 additional heteroatoms selected from O, S and N;
R a is H or C1-6 alkyl;
R b is NH2 or C1-6 alkyl unsubstituted or substituted with 1-3 groups selected from halo, OH, CN and C(O)NH2;
R c is independently selected from halo, OR a, SR a, OC(O)R a, CO2R a, CN, C(O)N(R a)2 and C(O)R a, R d is H or C1-3 alkyl, or R c and R d taken together with any intervening atoms represent a 4-6 membered ring;
R e is H; R c; NO2; N(R a)2; SO2N(R a)2 or C1-4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH
and C(O)NH2;
R f, R g and R h are independently selected from H; C1-6 straight or branched chain alkyl, unsubstituted or substituted with 1-3 R c groups; C3-6 cycloalkyl, unsubstituted or substituted with 1-3 R c groups; phenyl, unsubstituted or substituted with 1-3 R e groups and R f and R g taken together with the intervening N atom form a 4-6 membered ring, optionally interrupted by 1-2 of O, S, C(O) or NR h, and optionally substituted by 1-3 R c groups;
and each R independently represents H; NO2 ; N(R a)2;
SO2N(R a)2; R c or C1-4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH, C(O)NH2, or R together with A of the group -A-Q-L-B and any intervening atoms represent a 5-6 membered carbocyclic ring.

2. A compound in accordance with claim 1 wherein R1 represents methyl.

3. A compound in accordance with claim 1 wherein CO2M represents a carboxylate anion.

4. A compound in accordance with claim 1 wherein P represents hydroxyl or hydroxyl protected by a hydroxyl protecting group.

5. A compound in accordance with claim 1 wherein A represents C1-3 alkylene.

6. A compound in accordance with claim 1 wherein Q represents and X- represents a charge balancing group.

7. A compound in accordance with claim 1 wherein -C(O)NR a-Het(R e)-, -NR a-, -N+(R a)2 or

8. A compound in accordance with claim 1 wherein B represents

9. A compound represented by formula I

or a pharmaceutically acceptable salt or hydrate thereof wherein:
R1 represents methyl;
CO2M represents a carboxylate anion;
P represents hydroxyl or hydroxyl protected by a hydroxyl protecting group;
A represents C1-3 alkylene;
Q represents X- is a charge balancing counterion;

-C(O)NR d-, -C(O)NR a-Het(R e)-, -NR a-, -N+(R a)2 - or B represents: or -NR1R9.
R a is H or C1-6 alkyl;
R b is NH2 or C1-6 alkyl unsubstituted or substituted with 1-3 groups selected from halo, OH, CN and C(O)NH2;
R c is independently selected from halo, OR a, SR a, OC(O)R a, CO2R a, CN, C(O)N(R a)2 and C(O)R a, R d is H or C1-3 alkyl, or R c and R d taken together with any intervening atoms represent a 4-6 membered ring;
R e is H; R c; NO2; N(R a)2, SO2N(R a)2 or C1-4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH
and C(O)NH2;
R f, R g and R h are independently selected from H; C1-6 straight or branched chain alkyl, unsubstituted or substituted with 1-3 R c groups; C3-6 cycloalkyl, unsubstituted or substituted with 1-3 R c groups; phenyl, unsubstituted or substituted with 1-3 R e groups and Het, unsubstituted or substituted with 1-3 R e groups, or R f and R g taken together with the intervening N atom form a 4-6 membered ring, optionally interrupted by 1-2 of O, S, C(O) or NR h, and optionally substituted by 1-3 R c groups;
and each R independently represents H; NO2; N(R a)2;
SO2N(R a)2; R c or C1-4 alkyl, unsubstituted or substituted with 1-3 groups selected from halo, OH, C(O)NH2, or R together with A of the group -A-Q-L-B and any intervening atoms represent a 5-6 membered carbocyclic ring.

10. A compound represented by one of Tables I - III:

wherein Q-L-B is selected from:

wherein Q-L-B is selected from:

wherein Q-L-B is selected from:

wherein X- represents a counterion.

11. A pharmaceutical composition which is comprised of a compound in accordance with claim 1 in combination with a carrier.

12. A method of treating or preventing a bacterial infection in a mammalian patient in need thereof, comprising administering to said patient an antibacterially effective amount of a compound as described in claim 1.