CA2293593A1 - Procede automatise a haut debit permettant de cribler une pluralite de composes par spectrometrie de masse - Google Patents

Procede automatise a haut debit permettant de cribler une pluralite de composes par spectrometrie de masse Download PDF

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Publication number
CA2293593A1
CA2293593A1 CA002293593A CA2293593A CA2293593A1 CA 2293593 A1 CA2293593 A1 CA 2293593A1 CA 002293593 A CA002293593 A CA 002293593A CA 2293593 A CA2293593 A CA 2293593A CA 2293593 A1 CA2293593 A1 CA 2293593A1
Authority
CA
Canada
Prior art keywords
target
interest
compound
complex
mobile phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002293593A
Other languages
English (en)
Inventor
James N. Kyranos
Yu-Tysr Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arqule Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2293593A1 publication Critical patent/CA2293593A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00702—Processes involving means for analysing and characterising the products
    • B01J2219/00707—Processes involving means for analysing and characterising the products separated from the reactor apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

L'invention concerne un procédé qui permet de tester rapidement une pluralité de composés à la recherche d'une interaction non covalente avec au moins une cible. Le procédé comprend les étapes suivantes: on introduit un flux continu constitué d'une phase mobile dans un spectromètre de masse; on règle les paramètres dudit spectromètre de façon à détecter au moins une cible contenue dans la phase mobile; on injecte progressivement dans ladite phase mobile différents échantillons d'une pluralité de mélanges, chaque mélange comprenant au moins la cible et au moins un composé d'intérêt, de façon à les faire passer dans le spectromètre; et on obtient un spectre de masse qui indique pour chaque mélange la présence ou l'absence d'un complexe constitué de la cible et du composé d'intérêt. De préférence, on injecte la totalité de chaque échantillon dans la phase mobile en moins de 5 minutes, encore mieux en moins d'une minute et idéalement en moins de 30 secondes. On peut multiplexer le procédé de l'invention en constituant un mélange qui contient une seule cible et une pluralité de composés d'intérêt, ou bien un seul composé d'intérêt et une pluralité de cibles de protéines récepteurs ou de complexes récepteur-ligand. Typiquement, on forme la pluralité de mélanges au niveau d'une pluralité de sites adressables, tels que les puits d'une plaque multipuits. On retire les échantillons de mélanges des sites adressables, puis on les injecte progressivement dans la phase mobile de façon à les amener au spectromètre de masse. On peut également déterminer la force relative de l'interaction entre la cible et le composé d'intérêt au sein de chaque complexe en dissociant au moins un complexe constitué d'une cible et d'un composé d'intérêt.
CA002293593A 1997-06-03 1998-06-03 Procede automatise a haut debit permettant de cribler une pluralite de composes par spectrometrie de masse Abandoned CA2293593A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4841297P 1997-06-03 1997-06-03
US60/048,412 1997-06-03
PCT/US1998/011157 WO1998056028A1 (fr) 1997-06-03 1998-06-03 Procede automatise a haut debit permettant de cribler une pluralite de composes par spectrometrie de masse

Publications (1)

Publication Number Publication Date
CA2293593A1 true CA2293593A1 (fr) 1998-12-10

Family

ID=21954432

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002293593A Abandoned CA2293593A1 (fr) 1997-06-03 1998-06-03 Procede automatise a haut debit permettant de cribler une pluralite de composes par spectrometrie de masse

Country Status (4)

Country Link
EP (1) EP0990255A1 (fr)
AU (1) AU7713198A (fr)
CA (1) CA2293593A1 (fr)
WO (1) WO1998056028A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3920207A1 (fr) * 2010-12-28 2021-12-08 Quest Diagnostics Investments LLC Quantification de l'insuline par spectrométrie de masse

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191418B1 (en) 1998-03-27 2001-02-20 Synsorb Biotech, Inc. Device for delivery of multiple liquid sample streams to a mass spectrometer
US6066848A (en) * 1998-06-09 2000-05-23 Combichem, Inc. Parallel fluid electrospray mass spectrometer
US6919178B2 (en) 2000-11-21 2005-07-19 Sunesis Pharmaceuticals, Inc. Extended tethering approach for rapid identification of ligands
US6998233B2 (en) 1998-06-26 2006-02-14 Sunesis Pharmaceuticals, Inc. Methods for ligand discovery
US6355641B1 (en) 1999-03-17 2002-03-12 Syntex (U.S.A.) Llc Oxazolone derivatives and uses thereof
EP1048950A1 (fr) * 1999-04-26 2000-11-02 ScreenTec B.V. Identification de ligands de récepteurs orphelins mettant en jeu un couplage entre des techniques de spectrométrie de masse et de séparation à flux continu
EP1202056A1 (fr) * 2000-10-30 2002-05-02 Vrije Universiteit Amsterdam Essai biochimique homogène à écoulement continu reposant sur la détection de ligands connus par spectrométrie de masse
DE10125258A1 (de) 2001-05-23 2003-01-09 November Ag Molekulare Medizin Verfahren zur Bestimmung des Bindeverhaltens von an Ziel-Molekülen spezifisch bindenden Liganden
AU2003202103A1 (en) * 2002-01-03 2003-07-30 Arpad Furka Multi-target screening
US6847899B2 (en) 2002-04-26 2005-01-25 Dean Allgeyer, M.D., Inc. Device and method for qualitative and quantitative determination of intravenous fluid components
DE60322084D1 (de) * 2002-10-04 2008-08-21 Dean Owen Allgeyer Vorrichtung und verfahren zur qualitativen und quantitativen bestimmung von intravenösen flüssigkeitskomponenten
GB2526360B (en) * 2014-05-23 2018-04-04 Thermo Fisher Scient Bremen Gmbh Method and apparatus for mass spectrometry of macromolecular complexes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09507487A (ja) * 1994-01-05 1997-07-29 アーキュール，インコーポレーテッド 選ばれた性質を有するアミンイミドおよびオキサゾロンをベースとした分子の系統的モジュール製造
US5712171A (en) * 1995-01-20 1998-01-27 Arqule, Inc. Method of generating a plurality of chemical compounds in a spatially arranged array
WO1997001755A2 (fr) * 1995-06-26 1997-01-16 Perseptive Biosystems, Inc. Selection et analyse moleculaires multidimensionnelles automatiques et rapides a flux continu

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3920207A1 (fr) * 2010-12-28 2021-12-08 Quest Diagnostics Investments LLC Quantification de l'insuline par spectrométrie de masse

Also Published As

Publication number Publication date
EP0990255A1 (fr) 2000-04-05
WO1998056028A1 (fr) 1998-12-10
AU7713198A (en) 1998-12-21

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Legal Events

Date Code Title Description
FZDE Discontinued