CA2287862A1 - Cytotoxic compounds: derivatives of the pyrido[2,3,4-kl] acridine ring system - Google Patents

Abstract

Compounds of formula (I), wherein X is selected from the group consisting of O, and NR3, where R3 represents a lower alkyl group; Y is selected from the group consisting of CH and N; R1 and R2 are independently selected from the group consisting of NH2, NHR4 and NR52, where R4 and R5 each represent a lower alkyl group, or R1 and R2 together represent a cycle selected from (a), (b) and (c), wherein R6, R7 and R8 are independently selected from the group consisting of hydrogen atoms, lower alkyl groups, hydroxy groups and lower alkoxy groups; and Z is selected from the group consisting of O.

Description

CYTOTOXIC COMPOUNDS: DERIVATIVES OF THE
PYRiDO[2,3,4-kl]ACRIDINE RING SYSTEM
Background to the Invention The present invention relates to a series of new polycyclic aromatic alkaloids having a pyrido[2,3,4-k,l]acridine skeleton which have cytotoxic properties and which can therefore be used in the treatment of malignant tumours. The invention also provides methods and compositions using these new compounds as well as processes for their preparation.
The polycyclic aromatic alkaloids based on the pyrido[2,3,4-k,l]acridine skeleton are a growing class of ascidian metabolites that often exhibit a variety of interesting biological properties, including antitumour activity. l This class of compounds comprises three main structural types, depending of the position of the fusion between the parent structure and additional rings present in the natural product. For example, the cystoditines2 have the base skeleton mentioned above, while amphimedine,3 meridine4 and cystodamines bear an additional pyridine ring attached to the h bond; ascididemin,6 its derivatives, the kuanoniaminesg and shermilamine A9 show this additional ring at the i face, and eilatine at both.l~ Our target compounds can be regarded as regioisomers both of meridine and amphimedine, but they have not been so far isolated from natural sources.
SUBSTITUTE SHEET (RULE 26) /
\ N
\ ~ R
N
O
G~stoditincs Ann x=OH Meiidine x=NHi C'ystod>unine \ N ~ N
\ ~ O \ ~ O
i ~ i N ~~~ .N ~/ \S
N-Kuuaniaatino A
Hr H 2-Htamoleptoctinidioooe H ON 11-Hydtaxyucididmuoe (neoraHiutioe) $~~jl~j~ A
EIIi~IpE
Most of these prior art compounds exhibit very interesting cytotoxic properties towards a range of tumor cell lines. Although their mechanism of action is not clearly established, three general observations emerge from published biological data:ll a) they are intercalating agents; b) they disrupt -DNA and RNA synthesis, with little effect on protein synthesis; c) they inhibit topoisomerase II, which is the mechanism normally accepted for their antitumour activity.
Biological studies on pyridoacridines are severely limited due to their very low availability from natural sources, and therefore the study of their mechanism of action and the establishment of reliable structure-activity relationships requires the development of efficient synthetic routes.
SUBSTITUTE SHEET (RULE 26) Definition of the Invention We have now discovered a new family of polycyclic aromatic alkaloids having a pyrido[2,3,4-k,l]acridine skeleton which show excellent antitumour activity.
Thus, in a first aspect of the present invention there is provided compounds having the general formula (I):
i (I) wherein:
X is selected from the group consisting of O, and NR3, where R3 represents a lower alkyl group;
Y is selected from the group consisting of CH and N;
R' and R2 are independently selected from the group consisting of NH2, NHR4 and NRSZ.
where R4 and Rs each represent a lower alkyl group, or R1 and R2 together represent a cycle selected from (a), (b) and (c):
R6 R6 ~ N ~ Rg (a) ~ R~ (b) ~ R~ (c) . \. ~ \
Z O N Ra R6 SUBSTITUTE SHEET (RULE 26) wherein R6, R' and R8 are independently selected from the group consisting of hydrogen atoms, lower alkyl groups, hydroxy groups and lower alkoxy groups; and Z is selected from the group consisting of O and NH;
and pharmaceutically acceptable salts thereof.
In the definitions of the groups in formula (I), the lower alkyl groups and the lower alkyl moiety of the lower alkoxy groups are straight-chain or branched alkyl groups having 1 to 6 carbon atoms such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tent-butyl, pentyl, neopentyl and hexyl groups.
For those compounds of the present invention wherein R' and R2 together represent a cycle of formula (a), R6 and R' are preferably independently selected from the group consisting of hydrogen atoms, alkyl groups having from 1 to 4 carbon atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms; more preferably, R6 and R' are independently selected from the group consisting of hydrogen atoms, methyl groups and ethyl groups; and, most preferably, R6 represents a methyl group and R' represents a hydrogen atom. Furthermore, where R' and R2 together represent a cycle of formula (a), it is preferable that Z represents a group of formula NH.
For those compounds of the present invention wherein RI and R2 together represent a cycle of formula (b) or (c), R6, R' and Ra are preferably selected from the group consisting of hydrogen atoms, alkyl groups having from 1 to 4 carbon atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms; more preferably, R6, R' and R8 are independently selected from the group consisting of hydrogen atoms, methyl groups, ethyl groups and hydroxy groups; and, most preferably, R6 represents a hydroxy group and R' and Rg each represent a hydrogen atom.
In a preferred embodiment, the present invention relates to novel synthetic compounds of general structure (II) or (III):
SUBSTITUTE SHEET (RULE 26) w0 98/49165 PGT/GB98/01239 R~
O
H (II) R~
OH
(III) wherein R6, R' and R8 are as defined above.
Of the compounds of general formula (II), we particularly prefer the compound wherein R6 represents a methyl group and R' represents a hydrogen atom (Compound No.
IB-96213).
Of the compounds of general formula (III), we particularly prefer the compound wherein R' and R8 both represent a hydrogen atom (Compound No. IB-98205).
The present invention also provides a method for treating a mammal affected by a malignant tumor sensitive to a compound having the general formula (I), which comprises administering SUBSTITUTE SHEET (RULE 26) to the affected individual a therapeutically effective amount of the compound having the general formula (I) or a pharmaceutical composition thereof.
The present invention further provides pharmaceutical compositions, particularly useful in the treatment of malignant tumors, which contain as the active ingedient a compound having the general formula (I), as well as a process for the preparation of said compositions.
A further aspect of the present invention provides a method for preparing the compounds of general formula (I) and, in particular, Compounds Nos. IB-96213 and IB-98205.
Our strategy for the synthesis of the target compounds involves an hetero Diels-Alder reaction between an o-nitrogenated 4-aryl-1-dimethylamino-1,4-azadiene and a suitable heterocyclic quinone, followed by nucleophilic cyclization onto one of the quinone carbonyls (Scheme 1).
O
N

N
NMe=
ON = nitrogen function Scheme 1 Among the several 4-aryl-1-azadienes assayed, the best results were obtained for the o-nitro and the o-(trifluoroacetamido) derivatives. The first of them (compound la) was prepared from commercially available o-nitrocinnamaldehyde using a known proceduret2.
For the second azadiene (compound lb), two alternative syntheses were devised (Scheme SUBSTITUTE SHEET (RULE 26) 2). The first of them involved trifluoroacerylation of the corresponding o-amino derivative 2, prepared by reduction of compound 1a~2. In the second synthesis, o-aminobenzaldehyde . (3) was N-trifluoroacetylated and the amide was used as a Wadsworth-Emmons coupling partner with compound 5.13 / I /
(~ N \ H2 N \ F3 (CF3COn0 / /
N N
lb NMex NNIex la 2 O v ~HsO-~~ 5 CtIiso~ H
BuLi CF CO \
m N \ ( 3 ~O - Fj COCHN
CHO C

Scheme 2 The reaction between lb and quinones 614 to produce compounds of formula (II) was carried out in refluxing chloroform, and led to a mixture of the Diels-Alder adducts 7 and the secondary products 8. Compounds 7 were aromatized to 9 by refluxing in the presence of Pd-C in methanol solution, and 9 was finally transformed into the desired pentacyclic derivatives 10 [general structure (II)] by hydrolytic cyclization (Scheme 3).
SU9ST1TUTE SHEET (RULE 26) F3 \ R7 ~N O
O H
NMe2 lb 6 Me2 N R~
O
H O H O H

Pd-C

R~
HCI
O

Scheme 3 The use of 6-bromo-4-chloroquinolinequinones 11 as dienophiles led to reversal of the regioselectivity of the hetero Diels-Alder reaction. Thus, treatment of azadiene la with 11 under ultrasound irradiation afforded compound 12. Hydrogenation of 12 in the presence of Pd-C followed by workup with trifluoroacetic acid gave pyridoacridines 13 [general structure (III)] (Scheme 4).
SU9STITUTE SHEET (RULE 26) i OsN \ N Re / + ~ ~ - L) H=. Yd-C
2) CF~CO=H
Br ~ R~

O OH
la I1 1Z 13 Scheme 4 As examples, the detailed procedure for the synthesis of 12-methyl-9H benzo[b]
pyrido[4,3,2-d,a](1,7)phenantroline-8,10-dione, IB-96213 (general structure 10, where R6 - CH3, R7 - H) and 9-hydroxybenzo[b]pyrido[4,3,2-d,e](1,10)phenantrolin-8-one IB-98205 (general structure 13, where R7 = R8 = H) are given in the experimental section.
The compounds of the present invention are cytotoxic, compounds such as IB-96213 and IB-98205 exhibiting antitumor activity, especially against cell lines derived from human solid tumors, such as human lung carcinoma, human colon carcinoma and human melanoma, and, the like. They are also active against other tumor cell lines, like leukemia and lymphoma.
Compounds of formula (I), such as IB-96213 and IB-98205, have in vitro antitumor selectivity for solid tumors.
Examples of pharmaceutical compositions include any solid (tablets, pills, capsules, granules, etc.) or liquid (solutions, suspensions or emulsions) dosage form, with suitable formulation of oral, topical or parenteral administration, and they may contain the pure compound or in combination with any carrier or other pharmacologically active compounds. These compositions may need to be sterile when administered parenteraily.
The correct dosage of a phanmaceutical composition comprising compounds with formula (I), will vary according to the pharmaceutical formulation, the mode of application, and the particular silos, host and tumor being treated. Other factors like age, body weight, sex, diet, time of SUBSTITUTE SHEET (RULE 26) administration, rate of excretion, condition of the host, drug combinations, reaction sensitivities and severity of the disease shall be taken into account. Administration can be carried out continuously or periodically within the maximum tolerated dose.
Experimental The invention is further illustrated by the following Examples, which demonstrate the preparation of various of the compounds of the present invention. The reagents used were of commercial origin (Aldrich, Fluka) and were employed without further purification.
Solvents (SDS, Scharlau) were purified and dried by standard procedures.
Reactions were monitored by thin-layer chromatography, using Scharlau and Macherey-Nagel plates with fluorescent indicator. Separations by flash liquid chromatography were performed using silica gel SDS 60 ACC (230-400 mesh).
Melting points are uncorrected, and were determined in open capillary tubes, using a Buchi immersion apparatus or a Hoffler hot stage microscope. Combustion elemental analyses were obtained by the Servicio de Microanalisis Elemental, Universidad Complutense, using a Perkin Elmer 2400 CHN analyzer. Spectroscopic data were obtained with the following instruments: IR, Perkin Elmer 577 and Perki Elmer Paragon IR; NMR, Varian VXR-300 (300 MHz for 1H and 75 MHz for 13C) and Bruker AC-250 (250 MHz for 1H and 63 MHz for 13C).
Example 1 12-Methyl-9H benzolbl pyridof4.3.2-d.el(1.7~phenantroline-8.10-dione (IB-96213; a compound of formula 10 in which R6 = CH3 and R~-H) Steo 1 (a) 4-(o-Trifluoroacetamidophenyl)-1-dimethvlamino-1-azadiene (Formula lb SUBSTITUTE SHEET (RULE 26) Method A
Trifluoroacetic anhydride (1.39 g, 6.63 mmol) was added dropwise to a stirred solution of 4-(o-aminophenyl)-1-dimethylamino-1-azadiene (2)12 (837 mg, 4.42 mmol) in dry ethyl ' ether ( 10 ml). The solution was stirred at room temperature for 15 min and evaporated under reduced pressure at room temperature. The residue was purified by chromatography on silica gel, eluting with dichloromethane to give the title compound lb;
yield, 1.023 g (88 % ).
Data for 1b:
Mp, 110 °C
Analysis. Calcd. for C13H14F3N3: C, 54.73: H, 4.91; N, 14.73 Found: C, 54. i0; H, 4.19; N, 14.11 IR (KBr): 3250 (NH), 1655 (CO).
1H-NMR (250 MHz, CDC13) d: 8.01 (1H, s, NHCOCF3); 7.69 (1H, m, C3'-H); 7.52 (1H, m, C6-H); 7.25 (2H, m, C4,5-H); 7.08 (1H, d, J = 8.9 Hz, C2~-H); 6.88 (1H, dd, J
= 15.6 and 8.9 Hz, C3'-H); 6.50 (1H, d, J = 15.6 Hz, C4'-H); 2.88 (6H, s, CH3).
13C_NMR (63 MHz, CDC13) d: 155.14 (d, J = 37.1 Hz, CO); 133.43 (C2'); 1313.84 (C3'); 128.00 (C4); 127.54 (C5); 126.30 (C6); 124.61 (C3); 123.25 {C4');
115.66 (q, J =
288.0 Hz, CF3); 42.39 (NMe2).
Method B.
a) o-(trifluoroacetamido)benzaldehyde (4). To a suspension of o-nitrobenzaldehyde (3 g, 19.86 mmol) in 35 % aqueous hydrochloric acid {35 ml) was added 21 g (93.1 mmol) of tin (II) chloride in small portions. The suspension was stirred at room temperature for 72 h, neutralized with 6N aqueous sodium hydroxide and extracted with chloroform (4 x 50 ml).
The combined chloroform layers were dried over sodium sulphate and evaporated, yielding 1.87 g (78 % ) of o-aminobenzaldehyde 3. A pan of this residue ( I .26 g, 10.4 mmol) was dissolved in dry ethyl ether (5 ml). This solution was cooled to 0 °C
and treated dropwise with trifluoroacetic anhydride (2 ml, 14.15 mmol), while magnetically stirred.
The solution was stirred for 2 h at room temperature. The solvent was evaporated and the residue was SUBSTITUTE SHEET (RULE 26) chromatographed on silica gel, eluting with a 7:3 petroleum ether-dichloromethane mixture.
Yield, 1.65 g (77 % ) of compound 4.
Data for 4:
Mp, 71-72 °C -Analysis. Calcd. for C9H6F3N02: C, 49.78: H, 2.79; N, 6.45 Found: C, 49.70; H, 2.59; N, 6.11 1H-NMR (250 MHz, CDC13) d: 10.21 (1H, s, NHCOCF3); 9.99 (1H, m, C3'-H);
8.69 ( 1 H, d, J = 8.4 Hz, C3-H); 7.81 ( 1 H, dd, J = 7.6 and 5.6 Hz, C6-H);
7.72 ( 1 H, td, J = 7.9 and 1.6 Hz, C$-H); 7.41 (1H, t, J = 7.4 Hz, C4-H).
13C_NMR (63 MHz, CDCl3) d: 195.62 (CO); 155.70 (d, J = 38.1 Hz, CO); 138.05 (C1); 136.32 (C4); 136.04 (C5); 125.22 (C6); 122.47 (C2); 120.32 (C3); 115.44 (q, J =
289.2 Hz, CF3).
b) Wadsworth-Emmons reaction between compounds 4 and 5. To a stirred solution of hydrazone 513 (see Scheme 2) in dry tetrahydrofuran placed in a -78 °C
bath was added a solution of butyl lithium in hexanes (0.1 ml, 1.1 mmol). The coloured solution was stirred for 40 min at the same temperature and a solution of the aldehyde 4 (217 mg, 1 mmol) in dry tetrahydrofuran (1 ml) was then added. The reacting mixture was stirred at 0 °C for 4 h, quenched by addition of a saturated aqueous solution of ammonium chloride ( 10 ml) and extracted with chloroform (4 x 15 ml). The combined extracts were dried over sodium sulphate and evaporated and the residue was chromatographed on silica gel, eluting with 1:1 ethyl ether-petroleum ether. Yield, 103 mg (36 % ) of the title compound lb.
Step 1(b) 5-(o-Trifluoroacetamido)-4-meth~rl-5.8-dihydro-l,8-diazaanthracene-2,9.10-trione (a compound of formula 7 in which R6 = CH3 and RAH) A solution of quinone 6 (see Scheme 3; R6 - CH3, R' - H) 14a and azadiene lb produced according to Step 1 (a) above (32 mg, 0.17 mmol) in chloroform (50 ml) was refluxed in a bath at 60 °C for 22 h, while a stream of argon was forced through the SUBSTITUTE SHEET (RULE 26) solution. The reaction mixture was evaporated to dryness and the residue was purified by chromatography on silica gel, eluting with a gradient from 3:2 dichloromethane-ethyl acetate to neat ethyl acetate, yielding 25 mg (34 %) of the title 5,8-dihydro derivative 7 (R6= CH3, R' = H), 20 mg (28 %) of the aromatic compound 9 (see Scheme 3; R6=
CH3, R' = H) and 11 mg (28 % ) of compound 8 (see Scheme 3; R6 = CH3, R' =
H).14a Data for 7:
Mp 192 °C (AcOEt) Analysis. Calcd. for C21 H 14F3N304: C, 58.77: H, 3.36; N, 9.78 Found: C, 58.49; H, 3.62; N, 9.57 IR {KBr): 3364 (NH); 1726, 1661 (CO) cm-1.
1H-NMR (250 MHz, CDC13) d: 11.93 (1H, br. s, NH); 11.28 (1H, s, NHCOCF3);
9.30 (1H, br. s, NH) 7.37-7.32 (4H, m, C2~_6~-H); 6.50 (1H, s, C3-H); 6.35 (1H, m, C7-H); 4.96 ( 1 H, m, C6-H); 4.78 ( 1 H, d, J = 4.7 Hz, CS-H); 2.37 (3 H, s, CH3).
Data for 9:
Mp 285 °C
Analysis. Calcd. for C21H12F3N304: C, 59.04: H, 2.80; N, 9.83 Found: C, 59.22; H, 2.94; N, 9.59 IR (KBr): 3178 (NH); 1733, 1664 (CO) cm' 1.
1H-NMR (250 MHz, CDCl3) d: 10.00 (1H, br. s, NH); 9.02 (1H, d, J = 4.8 Hz, C7-H); 8.21 ( 1 H, s, NHCOCF3); 7.70 ( 1 H, d, J = 7.6 Hz, C6~-H); 7.58 ( 1 H, td, J = 7.6 and 1.5 Hz, C4~-H); 7.53 (1H, d, J = 4.8 Hz, C6-H); 7.42 (1H, td, J = 7.6 and 1.5 Hz, CS~-H); 7.16 (1H, dd, J = 7.6 and 1.5 Hz, C3'-H); 6.46 (1H, d, J = 1.1 Hz, C3-H);
2.46 (3 H, d, J = 1.1 Hz, CH3).
Step 1 (c) Oxidation of 7 to 9 To a suspension of compound 7 (R6 = CH3, R' = H), produced according to Step 1(b) above, [200 mg, 0.46 mmol in methanol (50 ml)] was added solid 10 % palladium on SUBSTITUTE SHEET (RULE 26) charcoal (100 mg, 9 mmol). The suspension was refluxed for 48 h, while vigorously stirred, and filtered through celite. The solvent was evaporated, yielding 136 mg (69 % ) of the title compound 9 (R6 = CH3, R' = H).
Step ltd) 12-Methvl-9H benzo[bl pyridof4.3.2-d.el(1,7)phenantroline-8.10-dione (IB-96213: compound of formula 10, R6 = CH3 and R' = H) To a solution of compound 9 (R6 = CH3, R' = H) produced according to Step 1 (d) above, in methanol ( 15 ml) was added 2 N aqueous hydrochloric acid (2 ml).
The solution was refluxed for 4 h, neutralized with 8 % aqueous sodium bicarbonate and extracted with chloroform. Evaporation of the organic layer gave 57 mg (78 % ) of the title compound 10 (R6 = CH3, R' = H).
Data for 10:
Mp > 300 °C (CHCl3) Analysis. Calcd. for C 19H 11 N3O2: C, 72. 86: H, 3.51; N, 13.41 Found: C, 72.87; H, 2.82; N, 13.57 IR (KBr): 3418 (NH); 1648 (CO and C=N) cm-1.
1H-NMR (250 MHz, F3C-C02D) d: 9.24 (1H, br. s, C6-H); 9.03 (iH, br. s, C5-H);
8.57 ( 1H, d, J = 7.7 Hz, C4-H); 8.22 ( 1 H, d, J = 7.7 Hz, C 1-H); 7.96 ( 1 H, t, J = 7.3 Hz, C2-H); 7.79 (1H, t, J = 7.3 Hz, C3-H); 6.45 (s, 1H, C"-H); 2.45 (s, 3H, C,2-CH3) ppm.
Example 2 SUBSTITUTE SHEET (RULE 26) 9-Hydroxybenzofbl.pvrido[4y3.2-d.e 1(1.10)phenantrolin-8-one (IB-98205: a compound of formula 13 in which R' = Rg= H) Step 2(a) 4-Chloro-8-(o-nitrophenyl)-1.5-diazaanthraquinone (a compound of formula 12 in which R' = Rg= H) A solution of quinone I1 (see Scheme 4; R7 = Rg = H)'S (185 mg, 0.68 mmol) and o-nitrocynammaldehyde dimethylhydrazone 1a~2 (438mg; 2 mmol) in chloroform (1 mL) was irradiated with ultrasound at 50 °C for 125 h. The solvent was evaporated and the residue was chromatographed on silica gel, eluting with ethyl acetate to give the title compound 11;
yield, 52 mg (20%).
Data for 12:
Mp , 146 °C
Analysis. Calcd. for C18H8C1N304: C, 59.13: H, 2.18; N, 11.48 Found: C, 59.89; H, 1.99; N, 11.17 IR (KBr)u: 1689 (C=O) cm-1.
1H-NMR (250 MHz, CDCl3) b: 9.15 (d, 1H, J = 4.8 Hz, H-6); 8.81 (d, 1H, J = 5.1 Hz, H-2); 8.34 (dd, 1 H, J = 7.6 and 1.3 Hz, H-3'); 7.77 (ddd, l H, J = 7.6, 7.6 and 1.3 Hz, H-5'); 7.72 (d, 1H, J = 5.1 Hz, H-3); 7.67 (ddd, 1H, J = 7.6, 7.6 and 1.3 Hz, H-4'); 7.49 (d, 1H, J = 4.8 Hz, H-7); 7.28 (dd, 1H, J = 7.6 and 1.3 Hz, H-6') ppm.
'3C-NMR (63 MHz, CDC13) b: 180.66, 179.54, 155.03, 154.05, 150.36, 149.86, 149.34, 146.91, 145.98, 134.15, 134.03, 131.06, 129.84, 129.59, 128.44, 127.09, 126.61, 124.93, 121.46 ppm Step 2(b) 9-Hvdroxybenzofbl pJrrido(4.3.2-d,e ](1.10)phenantrolin-8-one (13, R~-Rg = H) To a solution of compound 12 ( 100 mg, 0.27 mmol), produced according to Step 2(a) above, and triethylamine (37 mg) in methanol (25 mL) was added 10 % palladium on charcoal (22 mg). The suspension was hydrogenated at 1 atm for lh and filtered through SUBSTITUTE SHEET (RULE 26) WO 98/49165 PCf/GB98/O1Z39 celite. The celite layer was washed with a 2:1 mixture of trifluoroacetic acid and chloroform (25 mL). These washings were evaporated and chromatographed on silica gel, eluting with 9:1 ethyl acetate.methanol, affording 45 mg of a mixture of two reduction intermediates, whose structure was not determined. To a solution of 35 mg of this mixture in methanol (20 mL) was added 10 % palladium on charcoal (8 mg), and the suspension was hydrogenated at 1 atm for lh and filtered through celite. The solution was evaporated and the residue was chromatographed on silica gel, yielding 6 mg (10%) of the title compound 13.
'H-NMR (250 MHz, CF3COOH) 8: 9.30(d); 9.15(d); 8.73(d); 8.36(m); 8.10(m);
7.99(m); 7.48(d); 7.34(d) ppm.
The present inventors have prepared by the preceding synthetic pathway the following specific compounds, IB-96213 (general structure 10, were R6=CH3 and R7=H) and IB-98205 (general structure 13, were R7= Rg=H) which are specially preferred herein:
H

Biological Activity The compounds of formula (I) of the present invention show good antitumor activity. In particular, IB-96213 and IB-98205 display good antitumor activity against several mammalian cancer cell lines. Its antitumor activity has been detected in vitro by culturing the tumor cells SUBSTITUTE SHEET (RULE 26) following the methodology described by Bergeron et al. j6, and by Schroeder et a( '6. Activity against different tumors as mouse lymphoma, human NSC lung carcinoma, human melanoma and human colon carcinoma has been observed.
Some tumors were more sensitive than others. As for example it was found that NSC lung carcinoma and melanoma cells were 100 times more sensitive than mouse lymphoma and 1000 times more sensitive than human colon carcinoma cells.
EXAMPLE
Biological activity: Cells were maintained in logarithmic phase of growth in Eagle's Minimum Essential Medium, with Earle's Balanced Salts, with 2.0 mM L-glutamine, with non-essential amino acids, without sodium bicarbonate (EMEM/neaa); suplemented with 10% Fetal Calf Serum (FCS), 10-2 M sodium bicarbonate and 0,1 g/1 penicillin-G +
streptomycin sulfate.
The tumor cell lines employed have been P 388 (ATCC CCL-46, suspension culture of a lymphoid neoplasm from DBA/2 mouse), A-549 (ATCC CCL-185, monolayer culture of a human lung carcinoma), HT 29 (ATCC HTB-38, monolayer culture of a human colon carcinoma) and MEIr28 (ATCC HTB-38, tnonolayer culture of a human melanoma).
P-388 cells were seeded into 16 mm wells at 1x104 cells per well in 1 ml aliquots of MEM
SFCS containing the indicated concentration of drug. A separate set of cultures without drug was seeded as control growth to ensure that~cells remained in exponential phase of growth. All determinations were carried out in duplicate. After three days of incubation at 37°C, 10% C02 in a 98 % humide atmosphere, an aproximate ICso was determined by comparing the growth in - wells with drug to the growth in wells control.
' A-549, HT-29 and MEL-28 were seeded into 16 mm wells at 2x104 cells per well in 1 ml aliquots of MEM IOFCS containing the indicated concentration of drug. A
separate set of cultures without drug was seeded as control growth to ensure that cells remained in exponential SUBSTITUTE SHEET (RULE 26) phase of growth. All determinations were carried out in duplicate. After three days of incubation at 37 °C, 10 % C02 in a 98 % humide atmosphere, the wells were stained with 0.1 Crystal Violet. An aproximate ICSO was determined by comparing the growth in wells with drug to the growth in wells control.
In Table I are presented the cytotoxicity expressed as ICSO in ~cg/ml and ~M
TABLE I
ICS
wg/~(~1~'n CONIFOUND P 388 A-549 HT-29 MEIr28 IB-96213 0.1(0.32) 0.001(0.003) 1(3.18) 0.001(0.003) IB-98205 1.25(4.18) 0.012(0.04) 0.12(0.40) 0.05(0.17) SUBSTITUTE SHEET (RULE 26) REFERENCES
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SUBSTITUTE SHEET (RULE 26)

Claims (24)

Claims

1. A compound of formula (I):

wherein:
X is selected from the group consisting of O, and NR3, where R3 represents a lower alkyl group;
Y is selected from the group consisting of CH and N;
R1 and R2 together represent a cycle selected from (a) and (b):

wherein R6, R7 and R8 are independently selected from the group consisting of hydrogen atoms, lower alkyl groups, hydroxy groups and lower alkoxy groups; and Z is selected from the group consisting of O and NH;
and pharmaceutically acceptable salts thereof;
with the exclusion of a compound of formula (I) wherein X is O, Yis N, and R1 and R2 form a cycle (b) where each of R6, R7 and R8 is hydrogen.

2. A compound according to Claim 1, wherein X represent an oxygen atom.

3. A compound according to Claim 1 or Claim 2, wherein Y represent a nitrogen atom.

4. A compound according to any one of Claims 1 to 3, wherein R1 and R2 together represent a cycle of formula (a).

5. A compound according to any one of Claims 1 to 3, wherein R1 and R2 together represent a cycle of formula (b).

6. A compound according to Claim 4, wherein R6 and R7 are independently selected from the group consisting of hydrogen atoms, alkyl groups having from 1 to 4 carbon atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms.

7. A compound according to Claim 6, wherein R6 and R7 are independently selected from the group consisting of hydrogen atoms, methyl groups and ethyl groups.

8. A compound according to Claim 7, wherein R6 represents a methyl group and represents a hydrogen atom.

9. A compound according to any one of Claims 4 and 6 to 8, wherein Z
represents a group of formula NH.

10. A compound according to Claim 5, wherein R6, R7 and R8 are selected from the group consisting of hydrogen atoms, alkyl groups having from 1 to 4 carbon atoms, hydroxy groups and alkoxy groups having from 1 to 4 carbon atoms.

11. A compound according to Claim 10, wherein R6, R7 and R8 are independently selected from the group consisting of hydrogen atoms, methyl groups, ethyl groups and hydroxy groups.

12. A compound according to Claim 11, wherein R6 represents a hydroxy group and R7 and R8 each represent a hydrogen atom.

13. A compound according to Claim 1 having the following formula (II):

wherein R6 and R7 are as defined in Claim 1.

14. A compound according to Claim 13, wherein R6 represents a methyl group and represents a hydrogen atom.

15. A compound according to Claim 1 having the following formula (III):

wherein R7 and R8 are as defined in Claim 1.

16. A compound according to Claim 15, wherein R7 and R8 each represent a hydrogen atom.

17. A pharmaceutical composition comprising a compound of formula (I) as defined in any one of Claim 1 to 16 in admixture with a pharmaceutically acceptable carrier or diluent.

18. A pharmaceutical composition according to Claim 17 having antitumor activity.

19. A pharmaceutical composition according to Claim 18, for the treatment of malignant tumors.

20. A compound of formula (I) according to any one of Claims 1 to 16 for use in therapy.

21. Use of a compound of formula (I) according to any one of Claims 1 to 16 in the manufacture of a medicament for the treatment of malignant tumors.

22. A method for treating a mammal affected by a maligant tumor, comprising the administration to the affected individual of an effective amount of a compound of formula (I) according to any one of Claims 1 to 16.

23. A process for the proiduction of compounds of formula (II), as defined in Claim 13, comprising:
(a) reaction of a compound of formula lb:

with a compound of formula 6:

wherein R6 and R7 are as defined in Claim 1, to give a compound of formula 7:

wherein R6 and R7 are as defined in Claim 1;
(b) aromatization of the compound of formula 7 in the presence of a Pd-C
catalyst, to give a compound of formula 9:

wherein R6 and R7 are as defined in Claim 1; and (iii) hydrolytic cyclization of the compound of formula 9 to give the compound of formula (II).

24. A process for the production of a compound of formula (III), as defined in Claim 15, comprising:
(a) reaction of a compound of formula 1a:

with a compound of formula 11:

wherein R7 and R8 are as defined in Claim 1, to give a compound of formula 12:

wherein R7 and R8 are as defined in Claim 1; and (b) hydrogenation of the compound of formula 12 in the presence of a Pd-C
catalyst followed by acidic hydrolytic work-up, to give the compound of formula (III).