CA2285691A1 - Probes for the diagnosis of infections caused by bacteroides fragilis - Google Patents

Probes for the diagnosis of infections caused by bacteroides fragilis Download PDF

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Publication number
CA2285691A1
CA2285691A1 CA002285691A CA2285691A CA2285691A1 CA 2285691 A1 CA2285691 A1 CA 2285691A1 CA 002285691 A CA002285691 A CA 002285691A CA 2285691 A CA2285691 A CA 2285691A CA 2285691 A1 CA2285691 A1 CA 2285691A1
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CA
Canada
Prior art keywords
bacteroides fragilis
probes
diagnosis
infections caused
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002285691A
Other languages
French (fr)
Other versions
CA2285691C (en
Inventor
Hiroshi Ueyama
Kanako Abe
Hiroyuki Keshi
Akio Matsuhisa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuso Pharmaceutical Industries Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2285691A1 publication Critical patent/CA2285691A1/en
Application granted granted Critical
Publication of CA2285691C publication Critical patent/CA2285691C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The DNA from the bacteria Bacteroides fragilis is extracted, then completely digested with restriction enzyme HindIII, followed by cloning into a suitable vector to select a probe comprising DNA which is essentially contained in Bacteroides fragilis , then the sequence of the probe is elucidated.
CA002285691A 1997-03-25 1998-03-23 Probes for the diagnosis of infections caused by bacteroides fragilis Expired - Fee Related CA2285691C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP9071079A JPH10262676A (en) 1997-03-25 1997-03-25 Probe for diagnosis of infectious disease caused by bacteroides fragillis
JP9/71079 1997-03-25
PCT/JP1998/001287 WO1998042844A1 (en) 1997-03-25 1998-03-23 PROBES FOR THE DIAGNOSIS OF INFECTIONS CAUSED BY $i(BACTEROIDES FRAGILIS)

Publications (2)

Publication Number Publication Date
CA2285691A1 true CA2285691A1 (en) 1998-10-01
CA2285691C CA2285691C (en) 2007-05-08

Family

ID=13450166

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002285691A Expired - Fee Related CA2285691C (en) 1997-03-25 1998-03-23 Probes for the diagnosis of infections caused by bacteroides fragilis

Country Status (7)

Country Link
US (1) US6265568B1 (en)
EP (1) EP0995800A4 (en)
JP (1) JPH10262676A (en)
KR (1) KR100343106B1 (en)
AU (1) AU716843B2 (en)
CA (1) CA2285691C (en)
WO (1) WO1998042844A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022002506A (en) * 2021-04-16 2022-01-11 合同会社Ｈ．Ｕ．グループ中央研究所 One or more primers or probes specific for Bacteroides fragilis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4977251A (en) * 1987-01-30 1990-12-11 University Of Illinois Board Of Trustees DNA probes for identification of bacteroides species
EP0467049A1 (en) * 1990-07-16 1992-01-22 American Cyanamid Company DNA sequences and amino acid sequences of class b beta-lactamase enzymes from bacteroides fragilis

Also Published As

Publication number Publication date
AU6423098A (en) 1998-10-20
WO1998042844A1 (en) 1998-10-01
AU716843B2 (en) 2000-03-09
US6265568B1 (en) 2001-07-24
EP0995800A4 (en) 2004-08-04
EP0995800A1 (en) 2000-04-26
KR100343106B1 (en) 2002-07-05
CA2285691C (en) 2007-05-08
JPH10262676A (en) 1998-10-06
KR20010005667A (en) 2001-01-15

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MKLA Lapsed

Effective date: 20170323