CA2285263C - Furopyridine, thienopyridine, pyrrolopyridine and related pyrimidine, pyridazine and triazine compounds useful in controlling chemical synaptic transmission - Google Patents

Abstract

Novel heterocyclic ether compounds having formula (I) wherein A, m, R, X, Y1 , Y2 and Y3 are specifically defined, which are useful in selectively controlling chemical synaptic transmission; therapeutically- effective pharmaceutical compositions thereof; and use of said compositions to selectively control synaptic transmission in mammals.</SDOAB >

Description

Furopyridine, Thienopyridine, Pyrrolopyridine and Related Pyrimidine, Pyridazine and Triazine Compounds Useful in Controlling Chemical Synaptic Transmission This invention relates to furopyridine, thienopyridine, pyrrolopyridine and related pyrimidine, pyridazine and triazine compounds which control chemical synaptic transmission; to therapeutically effective pharmaceutical compositions of these compounds;
and to the use of said compositions to selectively control synaptic transmission.
BACKGROUND OF THE INVENTION
Compounds that selectively control chemical synaptic transmission offer therapeutic utility in treating disorders that are associated with dysfunctions in synaptic transmission.
This utility may arise from controlling either pre-synaptic or post-synaptic chemical transmission. The control of synaptic chemical transmission is, in tum, a direct result of a modulation of the excitability of the synaptic membrane. Presynaptic control of membrane excitability results from the direct effect an active compound has upon the organeIles and enzymes present in the nerve terminal for synthesizing, storing, and releasing the neurotransmitter, as well as the process for active re-uptake. Postsynaptic control of membrane excitability results from the influence an active compound has upon the cytoplasmic organelles that respond to neurotransmitter action.
An explanation -of the processes involved in chemical synaptic transn-dssion will help to illustrate more fully the potential applications of the invention. (For a fuller explanation of chemical synaptic transmission refer to Hoffman et al., "Neurot.ransmission:
The autonomic and somatic motor nervous systems." In: Goodman and Gilman's The PharmacoloQical Basis of Thera eutics, 9th ed., J.G. Hardman, L.E. Limbird, P.B. Molinoff, R.W.
Ruddon, and A. Goodman Gilman, eds., Pergamon Press, New York, 1996, pp.
105-139).
Typically, chemical synaptic transmission begins with a stimulus that depolarizes the transmembrane potential of the synaptic junction above the threshold that elicits an all-or-none action potential in a nerve axon. The action potential propagates to the nerve ternunal where ion fluxes activate a mobilization process leading to neurotransmitter SUBSTITUTE SHEET (RULE 26) secretion and "transmission" to the postsynaptic cell. Those cells which receive communication from the central and peripheral nervous systems in the form of neurotransmitters are referred to as "excitable cells." Excitable cells are cells such as nerves, smooth muscle cells, cardiac cells and glands. The effect of a neurotransmitter upon an excitable cell may be to cause either an excitatory or an inhibitory postsynaptic potential (EPSP or IPSP, respectively) depending upon the nature of the postsynaptic receptor for the particular neurotransmitter and the extent to which other neurotransmitters are present.
Whether a particular neurotransmitter causes excitation or inhibition depends principally on the ionic channels that are opened in the postsynaptic membrane (i.e., in the excitable cell).
EPSPs typically result from a local depolarization of the membrane due to a generalized increased permeability to cations (notably Na+ and K+), whereas IPSPs are the result of stabilization or hyperpolarization of the membrane excitability due to a increase in permeability to primarily smaller ions (including K+ and Cl-). For example, the neurotransmitter acetylcholine excites at skeletal muscle junctions by opening permeability channels for Na+ and K+. At other synapses, such as cardiac cells, acetylcholine can be inhibitory, primarily resulting from an increase in K+ conductance.
The biological effects of the compounds of the present invention result from modulation of a particular subtype of acetylcholine receptor. It is, therefore, important to understand the differences between two receptor subtypes. The two distinct subfamilies of acetylcholine receptors are defined as nicotinic acetylcholine receptors and muscarinic acetylcholine receptors. (See Goodman and Gilman's. The Pharmacological Basis of Therapeutics, op. cit.).
The responses of these receptor subtypes are mediated by two entirely different classes of second messenger systems. When the nicotinic acetylcholine receptor is activated, the response is an increased flux of specific extracellular ions (e.g. Na+, K+ and Ca++) through the neuronal membrane. In contrast, muscarinic acetylcholine receptor activation leads to changes in intracellular systems that contain complex molecules such as G-proteins and inositol phosphates. Thus, the biological consequences of nicotinic acetylcholine receptor activation are distinct from those of muscarinic receptor activation. In an analogous manner, inhibition of nicotinic acetylcholine receptors results in still other biological effects, which are distinct and different from those arising from muscarinic receptor inhibition.
As indicated above, the two principal sites to which drug compounds that affect chemical synaptic transmission may be directed are the presynaptic nerve ternunal and the postsynaptic membrane. Actions of drugs directed to the presynaptic site may be mediated through presynaptic receptors that respond to the neurotransmitter which the same secreting structure has released (i.e., through an autoreceptor), or through a presynaptic receptor that SUBSTITUTE SHEET (RULE 26) responds to another neurotransmitter (i.e., through a heteroreceptor). Actions of drugs directed to the postsynaptic membrane mimic the action of the endogenous neurotransmitter or inhibit the interaction of the endogenous neurotransmitter with a postsynaptic receptor.
Classic examples of drugs that modulate postsynaptic membrane excitability are the neuromuscular blocking agents which interact with nicotinic acetylcholine-gated channel receptors on skeletal muscle, for example, competitive (stabilizing) agents, such as curare, or depolarizing agents, such as succinylcholine.
In the central nervous system, postsynaptic cells can have many neurotransmitters impinging upon them. This makes it difficult to know the precise net balance of chemical synaptic transmission required to control a given cell. Nonetheless, by designing compounds that selectively affect only one pre- or postsynaptic receptor, it is possible to modulate the net balance of all the other inputs. Obviously, the more that is understood about chemical synaptic transmission in CNS disorders, the easier it would be to design drugs to treat such disorders.
Knowing how specific neurotransmitters act in the CNS allows one to speculate about the disorders that may be treatable with certain CNS-active drugs. For example, dopamine is widely recognized as an important neurotransmitter in the central nervous systems in humans and animals. Many aspects of the pharmacology of dopamine have been reviewed by Roth and Elsworth, "Biochemical Pharmacology of Midbrain Dopamine Neurons", In: Psvchopharmacologv: The Fourth Generation of Pro rg ess, F.E.
Bloom and D.J. Kupfer, Eds., Raven Press, NY, 1995, pp 227-243). Patients with Parkinson's disease have a primary loss of dopamine containing neurons of the nigrostriatal pathway, which results in profound loss of motor control. Therapeutic strategies to replace the dopamine deficiency with dopamine mimetics, as well as administering pharmacologic agents that modify dopamine release and other neurotransmitters have been found to have therapeutic benefit ("Parkinson's Disease", In: Psychopharmacology: The Fourth Generation of Progress, op. cit, pp 1479-1484).
New and selective neurotransmitter controlling agents are still being sought, in the hope that one or more will be useful in important, but as yet poorly controlled, disease states or behavior models. For example, dementia, such as is seen with Alzheimer's disease or Parkinsonism, remains largely untreatable. Symptoms of chronic alcoholism and nicotine withdrawal involve aspects of the central nervous system, as does the behavioral disorder Attention-Deficit Disorder (ADD). Specific agents for treatment of these and related disorders are few in number or non-existent.
A more complete discussion of the possible utility as CNS -active agents of compounds with activity as cholinergic ligands selective for neuronal nicotinic acetylcholine SUBSTITUTE SHEET (RULE 26) receptors, (i.e., for controlling chemical synaptic transmission) may be found in U.S. Patent 5,472,958, to Gunn et al., issued Dec. 5, 1995.
Existing acetylcholine agonists are therapeutically sub-optimal in treating the conditions discussed above. For example, such compounds have unfavorable pharmacokinetics (e.g., arecoline and nicotine), poor potency and lack of selectivity (e.g., nicotine), poor CNS penetration (e.g., carbachol) or poor oral bioavailability (e.g., nicotine). In addition, other agents have many unwanted central agonist actions, including hypothennia, hypolocomotion and tremor and peripheral side effects, including miosis, lachrymation, defecation and tachycardia (Benowitz et al., in: Nic ne PsychopharmacoloU, S. Wonnacott, M.A.H. Russell, & I.P. Stolerman, eds., Oxford University Press, Oxford, 1990, pp. 112-157; and M. Davidson, et al., in Curren Research in Alzheimer Therapy, E. Giacobini and R. Becker, ed.; Taylor &
Francis: New York, 1988; pp 333-336).
Additional conditions for which neurotransmitter controlling agents may be useful include acute and chronic pain. (A. Dray and L. Urban, Annu. Rev. Pharmacology Toxicol. 36: 253-280, (1996).
A 6-bromo-2-(1-piperidinyl)thieno[2,3-b)pyridine of indeterminate use was disclosed by Meth-Cohn et al., J. Chem. Soc., Perkin Trans., 1:2509-17 (1981).
Ciba-Geigy and Schenker et al. have disclosed various (2-benzofuranyl)-substituted tetrahydro pyridines and pyridines useful in treating mental depression (GB Patent No.
1,510,977, published May 17, 1978; and U.S. Patents No. 4,210,655 and 4,600,719). Toyama has disclosed N-BOC-thienopyridine derivatives having use an intermediates for preparation of complex cephalosporin-related antibiotic agents (PCT Patent Application WO
92/18505, published Oct. 29, 1992). Kabi Phannacia has disclosed bicyclic heteroaryl compounds attached to a quinuclidene moiety useful for treating diseases related to muscarinic receptor function (PCT Patent Application WO 93/23395, published Nov. 25, 1993). Festal et al.
have disclosed urea derivatives containing an azaindole moiety having utility as hypolipidemic and antiatheromatous agents (U.S.Patent No. 5,338, 849). Baker et al. have disclosed a class of substituted azetidine, pyrrolidine and piperidine derivatives having selective activity as agonists of 5-HTl-like receptors (PCT Patent Application WO
96/04274, published Feb. 15, 1996).

SUMMARY OF THE INVENTION
It has been found, in accordance with the present invention, that certain furopyridine, thienopyridine, pyrrolopyridine and related pyrimidine, pyridazine and triazine compounds are selective and potent cholinergic compounds useful in selectively controlling synaptic transmission.

SUBSTITUTE SHEET (RULE 26) In its principal aspect, the present invention provides a compound of formula (I) below, or a pharmaceutically acceptable salt thereof, wherein a monocyclic or bicyclic amine group is directly linked to a substituted or unsubstituted furopyridine, thienopyridine, pyrrolopyridine or related pyrimidine, pyridazine or triazine group.
5 Another aspect of the present invention provides pharrnaceutical compositions comprising a therapeutically effective amount of a compound of formula (I) in combination with a pharmaceutically acceptable carrier or diluent.
In yet another aspect, the present invention provides a method for selectively controlling synaptic transmission in a mammal.
The present invention relates to a compound of formula (I):

A / f i 2 Rm X Y3~Y
(I) or a pharmaceutically acceptable salt or pro-drug thereof wherein:
A is selected from the group consisting of:
(a) (CH2)n *
~

I

wherein * denotes a chiral center, n is 1,2or3, R1 is selected from the group consisting of H, allyl and Cl-C3-alkyl;
R2 is selected from the group consisting of H, C 1-C3-al.kyl, C1-C3-alkoxy, hydroxymethyl, fluoromethyl, methoxymethyl, and SUBSTITUTE SHEET (RULE 26) R2, when substituted at a position other than alpha to the ring nitrogen atom, is selected from Br, Cl, F, OH, CN, -0-CO-CH3 and-O-methanesulfonyl;
(b) (CH2)n/ N-CH2 \~\R2 (c) (C H2)p N

(CH2)q wherein p and q are independently I or 2;
(d) (CH2)P
N

(CH2)q wherein p and q are independently 1 or 2;
(e) N
R1 , and (f) R' I
N
wherein, in the case of (e) and (f) R 1 is as described above;

SUBSTITUTE SHEET (RULE 26) R is independently selected at each occurrence from the group consisting of C1-C4-alkyl, vinyl, bromo, chloro, fluoro, trifluoro-C 1-C4-alkyl, t.richloro-C 1-C4-alkyl, COOH, C02-C1-C4-alkyl, CN, nitro, amino, hydroxy, NH-CO-C1-C3-alkyl, and NR3R3, wherein R3 is H or C1-C3-alkyl; or when substituted at the Y2 position can additionally be selected from:
NR3R4, wherein R3 is H or C1-C3 alkyl and R4 is hydrogen, C1-Cg-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C 1-C6-alkyl-, substituted-phenyl-C l-C6-alkyl-, heteroaryl-C1-C6-a1ky1-, and substituted-heteroaryl-Cl-C6-alkyl-;
C(O)-R5, where R5 is hydrogen, C1-C8-alkyl, substituted-C1-C8-alkyl, phenyl, substituted phenyl, naphthyl, substituted naphthyl, heteroaryl, substituted heteroaryl, phenyl C1-C6alkyl-, substituted phenylCl-C6alkyl-, heteroaryl C1-C6alkyl-, substituted heteroaryl Cl-C6 alkyl-, and C1-C(,alkoxy-, NR6W, wherein R6 is selected from the group consisting of H and C1-C3-alkyl-, and R7 is selected from the group consisting of H, C1-C3-alkyl-, phenyl and substituted phenyl;
OR8, wherein R8 is C1-Cg-alkyl, phenyl, substituted phenyl, heteroaryl, substituted heteroaryl, phenylC1-C6alkyl-, substituted phenylCl-C6alkyl-, heteroaryl CI-C6alkyl-, CONR3R4;
phenyl;
naphthyl;
substituted phenyl;

SUBSTITUTE SHEET (RULE 26) substituted naphthyl;
biphenyl;
substituted biphenyl;
heteroaryl;
substituted heteroaryl;
phenyl Cl-C6alkyl-;
substituted phenylCI-C6alkyl-;
heteroaryl C1-C6alkyl-; and substituted heteroarylC1-C6alkyl-;
R9 , wherein R9 is selected from the group consisting of hydrogen, C1-C8alkyl, substituted C1-C8alkyl, phenyl, substituted phenyl, naphthyl, substituted naphthyl, heteroaryl, substituted heteroaryl, phenylCl-C6alkyl-, substituted phenylCl-C6alkyl-, heteroaryl C1-C6alkyl-, and substituted heteroarylCl-C6alkyl-;
'4~ Rs m , wherein m is I or 2, and R9 is as defined above;
-CH2-NH-CO-R5, wherein R5 is as defined above; and -CH2-CH2-CO-O-C I -C6-alkyl;
X is -0-, -S- or -NR3, wherein R3 is H or C1-C3-alkyl;

Y1, y2 and Y3 are N or CH, with the provisos that at least one of Y1, y2 and Y3 must be N and, when group A is selected from option (b), except for those compounds additionally substituted at Y2 above, then Y2 and y3 must be CH;
m, on formula (I), is 0, 1, 2 or 3. When m is zero or, at those positions around the 5-6 bicyclic ring system which are not substituted by R, hydrogen is the substituent.

The novel compounds of the present invention are also represented by formula (I):

A Rm X Y3~Y
(I) or a pharmaceutically acceptable salt or pro-drug thereof wherein the group designated A is selected from the group consisting of (a)-(f) as above:
the asterisk denotes a chiral center; m is 0, 1 or 2; n is 1, 2 or 3, and p and q are independently 1 or 2. The group R1 is selected from the group consisting of H
and Ci-C3-alkyl; and R2 is H, or when n is 2 or 3 is selected from the group consisting of Ci-C3-alkyl, C1-C3-alkoxyl, hydroxymethyl, fluoromethyl, methoxymethyl, Br, Cl, F, OH, CN, -CO-CH3 and -0-methanesulfonyl.
In the generic chemical structure shown above, R is independently selected at each occurrence from the group consisting of C1-C4-alkyl, bromo, chloro, fluoro, trifluoro-C1-C4-alkyl, trichloro-C1-C4-alkyl, COOH, C02-C1-C4-alkyl, CN, nitro, amino, NH-C3-alkyl, and NR3R3, wherein R3 is H or C1-C3-alkyl.
The group designated X is selected from the group consisting of -0-, -S- or -NR3, wherein R3 is H or Ci-C3-alkyl.
Y1, Y2 and Y3 are N or CH, with the provisos that at least one of Y1, Y2 and y3 must be N and when group A is selected from option (b), then Y2 and Y3 must be CH.
In yet another aspect of the invention, the invention relates to a compound of formula (III):

\
A Rm X Y3 Y2 1~1 R
(III) or a pharmaceutically acceptable salt or pro-drug thereof wherein:
A is selected from the group identified above; and R is independently selected at each occurrence from the group consisting of C1-C4-alkyl, vinyl, bromo, chloro, fluoro, trifluoro-C1-C4-alkyl, trichloro-CI-C4-alkyl, COOH, C02-C1-C4-alkyl, CN, nitro, amino, hydroxy, NH-CO-C1-C3-alkyl, and NR3R3, wherein R3 is H or C1-C3-alkyl:
and at the Y2 position R can additionally be selected from:
SUBSTITUTE SHEET (RULE 26) NR3R4, wherein R3 is H or C 1-C3 alkyl and R4 is hydrogen, C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C 1-C6-alkyl-, substituted-phenyl-C i -C6-alkyl-, 5 heteroaryl-C1-C6-alkyl-, and substituted-heteroaryl-C1-C6-alkyl-;
C(O)-R5, where R5 is hydrogen, C1-Cg-alkyl, substituted-C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, 10 heteroaryl-Ct-C6-alkyl-, substituted-heteroaryl-C1-C6-alkyl-, and O-C1-C6-alkyl-, N-R6R7, wherein R6 is selected from the group consisting of H and C1-C3-alkyl-, and R7 is selected from the group consisting of H, C1-C3-alkyl-, phenyl and substituted-phenyl;
OR8, wherein R8 is CI-Cg-alkyl, phenyl, substituted-phenyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, CONR3R4;
phenyl;
naphthyl;
substituted-phenyl;
substituted-naphthyl;
biphenyl;
substituted-biphenyl;
heteroaryl;
substituted-heteroaryl;
phenyl-C Z-C6-alkyl-;
substituted-phenyl-C1-C6-alkyl-;
heteroaryl-C1-C6-allcyl-; and substituted-heteroaryl-C 1-C6-alkyl-;
R9, wherein R9 is selected from the group consisting of hydrogen, C1-C8-alkyl, substituted-Ci-Cg-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-Cl-C6-alkyl-, substituted-phenyl-C 1-C6-alkyl-, heteroaryl-C 1-C6-alkyl-, and substituted-heteroaryl-Cl-C6-alkyl-;
SUBSTiTUTE SHEET (RULE 26) '~ R9 m, wherein m is 1 or 2, and R9 is as defined above;
-CH2-NH-CO-R5, wherein R5 is as defined above; and -CH2-CH2-CO-O-C1-C6-alkyl;
X is -0-, -S- or -NR3, wherein R3 is H or C1-C3-alkyl;

Y1, y2 and Y3 are N or CH, with the provisos that at least one of Y1, Y2 and Y3 must be N and, when group A is selected from option (b), except for those compounds additionally substituted at Y2 above, then Y2 and y3 must be CH
methoxymethyl or methoxymethoxy and m is 0, 1 or 2.
i5 DETAILED DESCRIP'1'ION OF THE INVENTION
Certain compounds of this invention may possess one or more asymmetric centers and may exist in optically active forms. Additional asymmetric centers may be present in a substituent group, such as an alkyl group. Compounds of the invention which have one or more asymmetric carbon atoms may exist as the optically pure enantiomers, pure diastereomers, mixtures of enantiomers, mixtures of diastereomers, racemic mixtures of enantiomers, diastereomeric racemates or mixtures of diastereomeric racemates.
It is to be understood that the present invention anticipates and includes within its scope all such isomers and mixtures thereof. The terms "R" and "S" used herein are configurations as defined in IUPAC 1974 Recommendations for Section E. Fundamental Stereochemistrv, Pure Appl. Chem. , 1976, 45: 13-30.
"Ci-C3-alkyl" and "Cl-C4-alkyl" refer to branched or straight-chain, unsubstituted alkyl groups comprising one-to-three or one-to-four carbon atoms, including, but not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, t-butyl and the like.
"Cl-C6-alkyI" or "Cl-Cg-alkyl" as used herein refer to saturated, straight- or branched-chain hydrocarbon radicals containing between one and six or one and eight carbon atoms, respectively. Examples of C1-C3 alkyl radicals include methyl, ethyl, propyl and isopropyl, examples of C1-C6-alkyl radicals include, but are not limited to, methyl, ethyl, propyl, isopropyl, n-butyl, tert-butyl, neopentyl, n-hexyl, and examples of C1-Cg-alkyl radicals include, but are not limited to, methyl, ethyl, propyl, isopropyl, n-butyl, tert-butyl, neopentyl, n-hexyl, n-heptyl and n-octyl.

SUBSTITUTE SHEET (RULE 26) "C1-C3-alkoxy" refers to a C1-C3-alkyl group, as defined above, containing an oxygen linker atom.
"Trichloro-Cl-C4-alkyl" refers to a C1-C4-alkyl group, as defined above, substituted with three chlorine atoms, including for example, trichloromethyl, 2,2,2-trichloroethyl, 3,3,3-trichloropropyl and 4,4,4-trichlorobutyl.
"Trifluoro-C1-C4-alkyl refers to a C1-C4-alkyl group, as defined above, substituted with three fluorine atoms, including for example, trifluoromethyl, 2,2,2-trifluoroethyl, 3,3,3-trifluoropropyl and 4,4,4-trifluorobutyl.
The term, "prodrug", refers to compounds that are rapidly transformed in vivo to yield the parent compounds of Formula (I), as for example, by hydrolysis in blood. T.
Higuchi and V. Stella provide a thorough discussion of the prodrug concept in Prodrugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series, American Chemical Society (1975). Examples of esters useful as prodrugs for compounds containing carboxyl groups may be found on pages 14-21 of Bioreversible Carriers in Drue Design:
Theorv and ADDliation, edited by E.B. Roche, Pergamon Press (1987).
The term, "prodrug ester group", refers to any of several ester-forming groups that are hydrolyzed under physiological conditions. Examples of prodrug ester groups include pivoyloxymethyl, acetoxymethyl, phthalidyl, indanyl and methoxymethyl, as well as other such groups known in the art.
The term, "administration", of the cholinergic agent or composition, as used herein, refers to systemic use as when taken oraily, parenterally, by inhalation spray, by nasal, rectal or buccal routes, or topically as ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or transdermal patches in dosage form unit formulations containing conventional nontoxic pharmaceutically acceptable carriers, adjuvants and vehicles as desired.
The term "parenteral", as used herein, includes intravenous, intramuscular, intraperitoneal, intrasternal, subcutaneous and intraarticular injection as well as via infusion techniques.
By "pharmaceutically acceptable", it is meant those salts, amides and esters which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and animals without undue toxicity, irritation, allergic response and the like, and are comrnensurate with a reasonable benefit/risk ratio, effective for their intended use in the treatment of psychological, neurological, cardiovascular and addictive behavior disorders.
Pharmaceutically acceptable salts are well known in the art. For example, S.
M. Berge, et al.
describe pharnaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66 : 1-19, 1977. The salts may be prepared in situ during the final isolation and purification of the compounds of Formula (I), or separately by reacting the free base function with a suitable SUBSTITUTE SHEET (RULE 26) acid. Representative acid addition salts include hydrochloride, hydrobromide, sulfate, bisulfate, acetate, oxalate, valerate, oleate, palmitate, stearate, laurate, borate, benzoate, lactate, phosphate, toluenesulfonate, methanesulfonate, citrate, maleate, fumarate, succinate, tartrate, ascorbate, glucoheptonate, lactobionate, lauryl sulfate salts and the like.
Representative alkali or alkaline earth metal salts include sodium, calcium, potassium, magnesium salts and the like. Examples of pharmaceutically acceptable, nontoxic amides of the compounds of Formula (I) include amides derived from Ci-C6-alkyl carboxylic acids wherein the alkyl groups are straight- or branched-chain, aromatic carboxylic acids such as derivatives of benzoic acid and heterocyclic carboxylic acids, including furan-2-carboxylic acid or nicotinic acid. Amides of the compounds of Formula (I) may be prepared according to conventional methods and include amino acid and polypeptide derivatives of the amines of Formula (I).
As used herein, the term, "pharmaceutically acceptable carriers", means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type. Some examples of the materials that may serve as pharmaceuticaliy acceptable carriers are sugars, such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc;
excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols such as glycerin, sorbitol, mannitol and polyethylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide;
alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol and phosphate buffer solutions, as well as other non-toxic compatible substances used in pharmaceutical formulations. Wetting agents, emulsifiers and lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants may also be present in the composition, according to the judgment of the formulator. Examples of pharmaceutically acceptable antioxidants include water-soluble antioxidants, such as ascorbic acid, cysteine hydrochloride, sodium bisulfite, sodium metabisulfite, sodium sulfite, and the like; oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, alpha-tocopherol and the like; and the metal chelating agents such as citric acid, ethylenediamine tetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid and the like.
By a"therapeutically effective amount" of the nicotinic acetylcholinergic agent, is meant a sufficient amount of the compound to treat cholinergically related disorders at a reasonable benefit/risk ratio applicable to obtain a desired therapeutic response. It will be SUBSTITUTE SHEET (RULE 26) understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder; activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient;
the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or coincidentally with the specific compound employed; and like factors well known in the medical arts.
Total daily dose of the compounds of this invention administered to a host in single or divided doses may be in amounts as determined by the attending physician, typically, for example, in amounts of from about 0.001 to 100 mg/kg body weight daily and preferably 0.01 to 10 mg/kg/day. Dosage unit compositions may contain such amounts of submultiples thereof to make up the daily dose.
The term "C1-C6-alkoxy" as used herein refers to an C1-C6-alkyl group, as previously defined, attached to the parent molecular moiety through an oxygen atom.
Examples of Cl-C6-alkoxy include, but are not limited to, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, tert-butoxy, neopentoxy and n-hexoxy.
The term "heteroaryl", as used herein, refers to a cyclic aromatic radical having from five to ten ring atoms of which one ring atom is selected from S, 0 and N;
zero, one or two ring atoms are additional heteroatoms independently selected from S, 0 and N;
and the remaining ring atoms are carbon, such as, for example, pyridinyl, pyrazinyl, pyridazinyl, pyr'lmidinyl, pyrrolyl, pyrazolyl, imidazolyl, indolyl, thiazolyl, oxazolyl, isoxazolyl, thiadiazolyl, oxadiazolyl, thienyl, furanyl, quinolinyl, isoquinolinyl, and the like, the heteroaryl moiety being joined to the rest of the molecule via any of its carbon ring atoms.
The term "heteroarylCl-C6alkyP" as used herein refers to a C1-C6-alkyl group as defined herein substituted by replacement of one of the hydrogen atoms thereon with a heteroaryl moiety, as defined above.
The term "phenylC1-C6alkyl" as used herein refers to a C1-C6-alkyl group as defmed herein substituted by replacement of one of the hydrogen atoms thereon with a phenyl moiety.
The term "substituted C1-Cgalkyl" as used herein refers to a C1-C8-alkyl group as defined herein substituted by independent replacement of one of the hydrogen atoms thereon with Cl, Br, F, CN, CF3, OH, CHO, COOH, COO-C1-C3-alkyl, C1-C6-alkoxy, methoxymethoxy, amino, or C1-C3-alkyl-amino, except not more than one CHO, COOH, or COO-C1-C3-alkyl group may be present.

SUBSTITUTE SHEET (RULE 26) The term "substituted biphenyl" as used herein refers to a biphenyl radical substituted by replacement of one of the hydrogen atoms thereon with F, OH, N02 or C 1-C3-alkyl.
The term "substituted naphthyl" as used herein refers to a naphthyl substituted by 5 independent replacement of one or two of the hydrogen atoms thereon with Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COO-C1-C3-alkyl, CI-C3-alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, or C1-C3-alkyl-amino, except not more than one CHO, COOH, or COO-C1-C3-alkyl group may be present.
The term "substituted phenyl" as used herein refers to a phenyl substituted by 10 independent replacement of one or two of the hydrogen atoms thereon with Cl, Br, F, CN, CF3, N02, OH, CHO, COOH, COO-C1-C3-alkyl, C1-C3-alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, or C1-C3-alkyl-amino, except not more than one CHO, COOH, or COO-C1-C3-alkyl group may be present.
The term "substituted phenylC1-C6alkyl" as used herein refers to a C1-C6-alkyl 15 group as defined herein substituted by replacement of one of the hydrogen atoms thereon with a substituted-phenyl moiety, as defined above.
The term "substituted heteroaryl" as used herein refers to a heteroaryl group as defined herein substituted on or two carbon atoms by independent replacement of he hydrogen atoms thereon with Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COO-CI-C3-alkyl, C1-C3-alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, or C1-C3-alkyl-amino, except not more than one CHO, COOH, or COO-C1-C3-al.kyl group may be present.
The tenm "substituted heteroaryl C1-C6alkyl" as used herein refers to a C1-C6alkyl group as defined herein substituted by replacement of one of the hydrogen atoms thereon with a substituted-heteroaryl moiety, as defined above.

Examples of compounds falling within the scope of the present invention precede the appended claims. If one specific enantiomer is shown or described, the other enantiomer may readily be made from the appropriate chiral precursor or can be resolved from a racemic mixture.

SUBSTtTUTE SHEET (RULE 26) In a preferred embodiment of the present invention, there are provided compounds of formula (II) N A f ! Rm X
II
wherein A is selected from the group consisting of (CH2)n ~"(CH2)p N
, N

~ ( )q R1 and CH3 and R' N
and the other variables as recited above for X and Rm.

In a particularly preferred embodiment of the present invention there is provided a compound of formula (II) above wherein A is selected from R' (CH2)n (CH2)P I
N
. > N N
RZ/N C H
R1 ( ~q , CH3 and wherein R is H, Br, Cl, C1-C4-alkyl, phenyl or vinyl pyridyl and R2 is H and RI is as specified above.
Further included within the scope of the present invention are pharmaceutical compositions comprising one or more of the compounds of formula (I) prepared and formulated in combination with one or more non-toxic pharmaceutically acceptable carriers in the manner described below.
Compositions suitable for parenteral injection may comprise pharmaceutically acceptable sterile aqueous or nonaqueous solutions, dispersions, suspensions or emulsions and sterile powders for reconstitution into sterile injectable solutions or dispersions.
Examples of suitable aqueous and nonaqueous carriers, diluents, solvents or vehicles SUBSTITUTE SHEET (RULE 26) include water, ethanol, polyols (propylene glycol, polyethylene glycol, glycerol, and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate. Proper fluidity may be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants.
These compositions may also contain adjuvants such as preserving, wetting, emulsifying, and dispersing agents. Prevention of the action of microorganisms may be ensured by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, and the like. It may also be desirable to include isotonic agents, for example, sugars, sodium chloride and the like. Prolonged absorption of the injectable pharmaceutical form may be brought about by the use of agents delaying absorption, for example, aluminum monostearate and gelatin.
If desired, and for more effective distribution, the compounds may be incorporated into slow-release or targeted-delivery systems, such as polymer matrices, liposomes, and microspheres. They may be sterilized, for example, by filtration through a bacteria-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions, which may be dissolved in sterile water, or some other sterile injectable medium immediately before use.
Solid dosage forms for oral administration may include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is admixed with at least one inert customary excipient (or carrier), such as sodium citrate or dicalcium phosphate, and additionally (a) fillers or extenders, as for example, starches, lactose, sucrose, glucose, mannitol and silicic acid; (b) binders, as for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose and acacia; (c) humectants, as for example, glycerol; (d) disintegrating agents, as for example, agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain complex silicates and sodium carbonate; (e) solution retarders, as for example paraffin; (f) absorption accelerators, as for example, quaternary ammonium compounds; (g) wetting agents, as for example, cetyl alcohol and glycerol monostearate; (h) adsorbents, as for example, kaolin and bentonite; and (i) lubricants, as for example, talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate or mixtures thereof. In the case of capsules, tablets and pills, the dosage forms may also comprise buffering agents.
Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules, using such excipients as lactose or milk sugar, as well as high molecular weight polyethylene glycols, and the like.
Solid dosage forms such as tablets, dragees, capsules, pills and granules may be prepared with coatings and shells, such as enteric coatings and others well known in this art.
SUBSTITUTE SHEET (RULE 26) They may contain pacifying agents, and may also be of such composition that they release the active compound or compounds in a certain part of the intestiinal tract in a delayed manner. Examples of embedding compositions which may be used are polymeric substances and waxes.
The active compounds may also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients.
Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms.may contain inert diluents commonly used in the art, such as water or other solvents, solubilizing agents and emulsifiers, as for example, ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils, in particular, cottonseed oil, groundnut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan or mixtures of these substances, and the like.
Besides such inert diluents, these liquid dosage forms may also include adjuvants, such as wetting agents, emulsifying and suspending agents, sweetening, flavoring and perfuming agents.
Suspensions, in addition to the active compounds, may contain suspending agents, as for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar and tragacanth, or mixtures of these substances, and the like.
Compositions for rectal or vaginal administrations are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax, which are solid at ordinary temperatures but liquid at body temperature and therefore, melt in the rectum or vaginal cavity and release the active component.
Dosage forms for topical or transdermal administration of a compound of this invention further include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or transdermal patches. Transdermal administration via a transdermal patch is a particularly effective and preferred dosage form of the present invention. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservative, buffers or propellants as may be required. It is known that some agents may require special handling in the preparation of transdermal patch formulations. For example, compounds that are volatile in nature may require admixture with special formulating agents or with special packaging materials to assure proper dosage delivery. In addition, compounds which are very rapidly absorbed through the skin may SUBSTITUTE SHEET (RULE 26) require formulation with absorption-retarding agents or barriers. Ophthalmic formulations, eye ointments, powders and solutions are also contemplated as being within the scope of this invention.
The present compounds may also be administered in the form of liposomes. As is known in the art, liposomes are generally derived from phospholipids or other lipid substances. Liposomes are formed by mono- or multi-lamellar hydrated liquid crystals that are dispersed in an aqueous medium. Any non-toxic, physiologically acceptable and metabolizable lipid capable of forming liposomes may be used. The present compositions in liposome form may contain, in addition to the compounds of the present invention, stabilizers, preservatives, excipients, and the like. The preferred lipids are the phospholipids and the phosphatidylcholines (lecithins), both natural and synthetic. Methods to form liposomes are known in the art. See, for example, Prescott, Ed., Methods in Cell Biologv. Volume XIV, Academic Press, New York, N. Y., (1976), p 33 et seq.
In order to reduce unwanted peripherally mediated side-effects, it is advantageous, but not essential, to incorporate into the composition a peripherally acting anti-cholinergic such as N-methylscopolamine, N-methylatropine, propantheline, methantheline, or glycopyrrolate.
The compounds of the present invention may be synthesized as shown in reaction schemes 1-23 presented below using the reactions and techniques described in this section.
The reactions are performed in a solvent appropriate to the reagents and materials employed are suitable for the transformation being effected. It is understood by those skilled in the art of organic synthesis that the functionality present on the heterocyclic ring and other portions of the molecule must be consistent with the chemical transformation proposed.
This will, on occasion, necessitate judgment by the routineer as to the order of synthetic steps, protecting groups required, and deprotection conditions. Substituents on the starting materials may be incompatible with some of the reaction conditions required in some of the methods described, but alternative methods and substituents compatible with the reaction conditions will be readily apparent to skilled practitioners in the art. The use of nitrogen-protecting groups is well known in the art for protecting amino groups against undesirable reactions during a synthetic procedure and many such protecting groups are known, cf, for example, T.H. Greene and P.G.M. Wuts, Protective Groups in Organic Synthesis, 2nd edition, John Wiley & Sons, New York (1991).

SUBSTiTUTE SHEET (RULE 26) Scheme l i N\
+ I
11 HO R)m jl N

(R)m n H O ~ \ -'.'. N N
\ (R) Fi' 4 5 ---~ (R)m In accordance with Scheme 1 are prepared furo[3,2-b)pyridine compounds of Formula (I) wherein A is selected from group (a), R, R i and R2 are as described above, X
5 is 0, Y1 is N and Y2 and Y3 are CH. The process may be illustrated with the pyrrolidine series (n=2) thereof, in which an N-protected 2-acetylenylpyrrolidine starting material (1), wherein P is a N-protecting group, such as for example, BOC or CBZ, (which may be prepared from the corresponding imino-2-carboxylic acids according to known methods (Garvey, et al., J. Med. Chem., 35: 1550-1557, 1992)) is reacted with an appropriate 2-10 iodo-3-hydroxypyridine (2), wherein R is as described above, in the presence of Pd, Cul and triethylamine at elevated temperature, to give the compound (3). (See Kundu, et al., J.
Chem. Soc. Chem. Comm., 1992: 41 for analogous preparation of benzofurans).
The protecting group P may then be removed by standard methods to give compound (4), i.e., compounds of fonnula (I) wherein R1 is H. Compound (4) may be converted into i s compounds (5), i.e., compounds of formula (I) wherein R1 is C1-C3-alkyl by reaction with the appropriate aldehyde under reducing conditions, for example, in the presence of H2 and a catalyst such as Pd/C or in the presence of NaBH3CN. The process of Scheme 1 is equally applicable to compounds of the series wherein n is 1 or 3, to give compounds analogous to compounds (4) and (5), i.e., compounds of formula (I) wherein A
is (a) and n 20 is 1 or3.
Alternately, for compounds of Formula (I) wherein X is S, compounds are prepared by appropriate modifications of the above schemes for X = O. The appropriate precursor o-halo-hydroxyheterocycles are converted to the corresponding o-halo-mercaptoheterocycles by reaction with a diakylthiocarbamyl chloride, for example diethyl thiocarbamyl chloride, followed by heating to effect rearrangement to the thiocarbamate, followed by hydrolysis (Kwart and Evans, J. Org. Chem., 31: 410, 1966; Newman and Karnes, Org. Syn., SUBSTITUTE SHEET (RULE 26) 51:139, 1971). The resultant o-halo-mercaptoheterocycles are then allowed to react with the acetylene compound (1) under copper catalysis (optionally in the presence of palladium) at elevated temperature to afford thieno-fused heterocycles (cf. Malte and Castro, J. Am.
Chem. Soc., 89: 6770, 1967). Such reactions may be applied to give the desired starting materials wherein 0 is replaced by S for the compounds described in the following schemes, also.

Scheme 2 I N
^ ^ /
(CH2)N-CH2 + I ~~ --- (CH2)N-CH2 N
R2 H 0 ~~R)m R2 O ~, ~R)m In accordance with Scheme 2 are prepared furo[3,2-b]pyridine compounds of Formula (I) wherein A is selected from group (b), R2 is as described above, X
is 0, Y 1 is N
and Y2 and Y3 are CH. The process may be illustrated with the pyrrolidine series (n=2) thereof, in which a 1-(3-propynyl)pyrrolidine starting material (6) (which may be prepared by reaction of the appropriately substituted pyrrolidine with 3-bromopropyne under basic conditions; see, for example, Biehl and DiPierro, J. Am. Chem. Soc., $Q:4609-4614, 1958). The compound (6) is reacted with an appropriate 2-iodo-3-pyridinol (2), wherein R is as described above, in the presence of Pd, CuI and triethylamine at elevated temperature, to give the compound (7). The process of Scheme 2 is equally applicable to compounds of the series wherein n is I or 3, to give compounds analogous to compound (7), i.e., compounds of formula (I) wherein A is (b) and n is 1 or 3.
The 2-iodo-3-pyridinois of Schemes 1 and 2 may be prepared by direct selective iodination of the corresponding pyridinols (e.g., Koch and Schnatterer, Synthesis, 1990:497). Alternately, 3-pyridinols with substituents in the 4-position can be prepared by selective lithiation of 3-pyridinol, 0-protected with an ortho-directing moiety, e.g.
methoxymethyl , diethylcarbamoyl, and the like (see Beak and Snieckus, Acc.
Chem. Res., 15:306-312, 1982). Alternately, 3-hydroxypyridines with substituents in other required positions can be prepared from the corresponding 3-aminopyridines under diazotizing conditions. Where appropriate, the 3-aminopyridines can be obtained by reduction of the corresponding 3-nitropyridine or by rearrangement of the 3-carboxylic acid or carboxamide using the Hoffman, Curtius, or Schmidt rearrangements which are well-known in the art. In addition, 3-hydroxypyridines can be obtained by oxidation of an appropriate 3-SUBSTITUTE SHEET (RULE 26) lithio or magnesiopyridine with molecular oxygen, oxaziridines, or peroxides (see, for example, Taddei and Ricci, Syn. Comm., 1986:633-635), or alternately peroxide oxidation of a pyridyl-3-dialkylborate, which can be obtained by reaction of a trialkyl borate with the appropriate 3-lithio- or magnesiopyridine (cf. Lawesson and Yang, J. Am. Chem.
Soc., 81:4320, 1959, and/or Hawthorne, J. Org. Chem., 22:1001, 1957).
In an alternate procedure, the reactions of Scheme 2 may be performed with the analogous mercaptopyridine, prepared as described for Scheme 1 above, to give the thieno[3,2-b]pyrimidine compounds of Formula (I), wherein X is a S atom.

Scheme 3 1 or 6 + -(R)m / :]CT H O ~N A O (R)m In accordance with Scheme 3 are prepared furo[2,3-c]pyridine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R 1 and R2 are as described above, X is 0, Y1 is CH, Y2 is N and Y3 is CH. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 4-iodo-3-hydroxypyridine (9), wherein R is as described above, in the presence of Pd, Cul and triethylamine at elevated temperature, to give the compound (10). The requisite 4-iodo-3-hydroxypyridines are generally available using the techniques mentioned above together with selective 4-iodination of 3-hydroxypyridines (cf. Winkle and Ronald, J. Org. Chem., 47:2101, 1982). In a further alternate procedure, the reactions of Scheme 3 may be performed with the analogous mercaptopyridine, prepared as described for Scheme 1 above, to give the thieno[2,3-c]pyrimidine compounds of Formula (I), wherein X is a S atom.

Scheme 4 1 or 6 + ~ (R)m A ~
H O II(R)m N~ O Ni In accordance with Scheme 4 are prepared furo[2,3-b]pyridine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, Ri and R2 are as described above, X is 0, Y 1 and Y2 are CH and Y3 is N. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 3-iodo-2-hydroxypyridine (11), wherein R is as SUBSTITUTE SHEET (RULE 26) described above, in the presence of Pd, CuI and triethylamine at elevated temperature, to give the compound (12). The requisite 3-iodo-2-hydroxypyridines are generally available using the techniques mentioned above for synthesis of selectively substituted hydroxypyridines. For example, the requisite 3-iodo-2-hydroxypyridines can be obtained by ortho iodination of the appropriate 2-hydroxypyridine. In a further alternate procedure, the reactions of Scheme 4 may be performed with the analogous mercaptopyridine, prepared as described for Scheme 1 above, to give the thieno[2,3-b]pyrimidine compounds of Formula (I), wherein X is a S atom.

Scheme 5 1 or6 + I I ~ (R)m ~
N~
HO N A I ~(R)m O

In accordance with Scheme 5 are prepared furo[3,2-d]pyrimidine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, Rl and R2 are as described above, X is 0, Y1 is N, Y2 is N and Y3 is CH. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 4-iodo-5-hydroxypyrimidine (13), wherein R is as described above, in the presence of Pd, Cul and triethylamine at elevated temperature, to give the compound (14). The requisite 4-iodo-5-hydroxypyrimidine compounds are generally available using the techniques mentioned above for synthesis of selectively substituted 3-hydroxypyridines. For example, the requisite 4-iodo-5-hydroxypyrimidine can be obtained by ortho iodination of the appropriate 5-hydroxypyrimidine. In a further alternate procedure, the reactions of Scheme 5 may be performed with the analogous mercaptopyridine, prepared as described for Scheme 1 above, to give the thieno[3,2-d]pyrimidine compounds of Formula (I), wherein X is a S atom.
Scheme 5A

1 oY 6 + ` I N~ (R)m _i. ~ N~
H O N~ A O ~ ~{R)m N

SUBSTITUTE SHEET (RULE 26) In accordance with Scheme 5A are prepared furo[2,3-b]pyrimidine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is 0, Y1 is N, Y2 is N and Y3 is CH. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 3-iodo-2-hydroxypyrirnidine (13A), wherein R is as described above, in the presence of Pd, CuI and triethylamine at elevated temperature, to give the compound (14A). The requisite 3-iodo-2-hydroxypyrimidine compounds are generally available using the techniques mentioned above for synthesis of selectively substituted 2-hydroxypyridines. For example, the requisite 3-iodo-2-hydroxypyrimidine can be obtained by ortho iodination of the appropriate 2-hydroxypyrimidine. In a further altemate procedure, the reactions of Scheme 5A may be performed with the analogous mercaptopyridine, prepared as described for Scheme 1 above, to give the thieno[2,3-b]pyrimidine compounds of Formula (I), wherein X is a S atom.

Scheme 6 ~
1 Or 6 + ---(R)m lm A ( ~(R)m H N p N~N

In accordance with Scheme 6 are prepared furo[2,3-c]pyridazine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is 0, Y1 is CH, and Y2 and Y3 are N. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 4-iodo-3-hydroxypyridazine (15), wherein R is as described above, in the presence of Pd, Cul and triethylamine at elevated temperature, to give the compound (16). The requisite 4-iodo-3-hydroxypyridazine compounds are generally available using the techniques mentioned above for synthesis of selectively substituted 3-hydroxypyridines. For example, the requisite 4-iodo-3-hydroxypyridazine can be obtained by ortho iodination of the appropriate 5-hydroxypyridazine. In a further altemate procedure, the reactions of Scheme 6 may be performed with the analogous mercaptopyridine, prepared as described for Scheme I above, to give the thieno[2,3-c]pyridazine compounds of Formula (I), wherein X is a S atom.

SUBSTiTUTE SHEET (RULE 26) Scheme 7 I Ny R NR
1 or6 + Y
~ --' A ~
H O N p N~N

In accordance with Scheme 7 are prepared furo[3,2-e]triazine compounds of 5 Formula (I) wherein A is selected from (a) or (b) above, R, R 1 and R2 are as described above, X is 0, Y1 is CH, Y2 is N and Y3 is N. The acetylene-substituted starting material (1) or (6) is reacted with an appropriate 5-iodo-6-hydroxytriazine (17), wherein R is as described above, in the presence of Pd, Cul and triethylamine at elevated temperature, to give the compound (18). The requisite 5-iodo-6-hydroxytriazine compounds are generally 10 available using the techniques mentioned above for synthesis of selectively substituted 3-hydroxypyridines. For example, the requisite 5-iodo-6-hydroxytriazine can be obtained by ortho iodination of the appropriate 5-hydroxytriazine.
Alternately, this reaction may be performed with the analogous mercaptopyrimidine, prepared as described for Scheme 1 above, to give the thieno[3,2-e]triazine compounds of 15 Formula (I), wherein X is a S atom.

Scheme 8 H2N N ~ I ~ N
I / (R)m (R)m 1 or6 A (R)m N
H

20 In accordance with Scheme 8 are prepared pyrrolo[3,2-b]pyridine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Y1 is N and Y2 and Y3 are CH. A starting material amino-nitro pyridine (19) is reacted with NaNO2 and HI to replace the amino group with an iodo group, then with iron and acetic acid to reduce the nitro group to an amino group and give the compound 25 (20). Compound 20 is then reacted with compound I or 6 in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, to give the compound (21).
SUBSTITUTE SHEET (RULE 26) Schme 9 / (R)m (R)m 1 ~ 7 or 6 ~ N 1 ~ N
RyNH RyN

A (R)m I iN
N
H

In accordance with Scheme 9 are prepared pyrrolo[2,3-c]pyridine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Y1 and Y3 are CH and Y2 is N. A protected aminopyridine compound starting material (22) is reacted with a strong base, such as t-butyllithium, and free iodine to give the iodinated compound (23). Compound (23) is then reacted with compound (1) or (6) in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, followed by N-deprotection under standard conditions, to give the compound (24).
Scheme 10 (R)m I / (R)m R. 'Y NH N Ry NH N

A ~ I~(R)m 1) 1 or 6 N N % 2) deacylation H

In accordance with Scheme 10 are prepared pyrrolo[2,3-b]pyridine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Y 1 and Y2 are CH and Y3 is N. A protected aminopyridine compound starting material (25) is reacted with a strong base, such as t-butyllithium, and free iodine to give the iodinated compound (26). Compound (26) is then reacted with compound (1) or (6) in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, then deacylated by standard methods to give the compound (27).

SUBSTITUTE SHEET (RULE 26) Scheme 11 N(R)m I N (R)m R l NH 'R NH
~
Ol ~ 0 N
N(R)m 1) 1 or 6 N i 2) deacylation In accordance with Scheme 11 are prepared pyrrolo[3,2-d]pyrimidine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Yl and Y2 are N, and Y3 is CH. A protected aminopyrimidine compound starting material (28) is reacted with a strong base, such as t-butyllithium, and free iodine to give the iodinated compound (29). Compound (29) is then reacted with compound (1) or (6) in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, then deacylated by standard methods to give the compound (30).

Scheme 12 (R)m (R)m 'R NH N'N ~ R NH N,N
~ 31 ~ 32 q (R)m 1) 1or6 ~. ~
N ~N 2) deacylation In accordance with Scheme 12 are prepared pyrrolo[2,3-c]pyridazine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Y 1 is CH, and Y2 and Y3 are N. A protected aminopyridazine compound starting material (31) is reacted with a strong base, such as t-butyllithium, and free iodine to give the iodinated compound (32). Compound (32) is then reacted with compound (1) or SUBSTiTUTE SHEET (RULE 26) (6) in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, then deacylated by standard methods to give the compound (33).

Scheme 13 N R I NYR
'Y ' R NH N'N 'R1 NH `N,N
0 34 ~ 10 35 N R
A a,, Y 1) 1 or6 N.N 2) deacylation In accordance with Scheme 13 are prepared pyrrolo[3,2-e]triazine compounds of Formula (I) wherein A is selected from (a) or (b) above, R, R1 and R2 are as described above, X is NH, Yt is CH, and Y2 and Y3 are N. A protected aminotriazine compound starting material (34) is reacted with a strong base, such as t-butyllithium, and free iodine to give the iodinated compound (35). Compound (35) is then reacted with compound (1) or (6) in the presence of Pd, Cul and triethylamine at elevated temperature, as described above, then deacylated by standard methods to give the compound (36).

Scheme 14 ~ BrMg--H _ N N ---+

In accordance with Scheme 14 is prepared the 7a-ethynyl-lH-hexahydropyrrolizine starting material for compounds of Formula (I) wherein A is selected from option (c). The starting material pyrrolizidinium compound (prepared according to the procedure of Miyano et al., Synthesis, 197g:701-2) is reacted with the ethynyl magnesium bromide under appropriate Grignard conditions to give the compound (38). Compound 38 may be substituted for compounds (1) or (6) in any of Schemes 1-13 above to give the desired compound of Formula (I).

SUBSTITUTE SHEET (RULE 26) Scheme 15 CH2OH -.- C36"w CHO
N N
I I
Boc Boc 1. Ph3P=CHCHO
CHO
N 2. H2/Pd I
Boc H
N N
I
Boc Br In accordance with Scheme 15 is prepared the 3-ethynyl-lH-hexahydropyrrolizine starting material (43) for compounds of Formula (I) wherein A is selected from option (d).
The protected prolinol (39) is converted to the aldehyde compound (40) by reaction with triethylamine and pyridine=sulfur trioxide complex in DMSO. Compound (40) is reacted with (triphenylphosporanylidene)acetaldehyde, followed by reduction of the intermediate with H2 over a Pd/C catalyst to give the extended aldehyde compound (41).
Compound (41) is subsequently reacted with, for example, ethynyl magnesium bromide and the intermediate is reacted with triphenylphosphine dibromide to give compound (42).
Compound 42 is treated with HCI in a polar organic solvent, such as ethanol, for example to give the 3-substituted pyrrolizidine compound (43). Compound (43) may be substituted for compounds (1) or (6) in any of Schemes 1-13 above to give the desired compound of Formula (I).

SUBSTITUTE SHEET (RULE 26) Scheme 16 C02Et 1. 4 atm H2, 20% Pd/C 1. LiAIH4 2. HCI / Et20 C02Et 2, pyridine=S03 ' N H 3. (BOC)2O J'.. 1 H
Me Boc ~

1. CBr4, PPh3 H
C H O 2, n-BuLi N H N H
Boc Boc In accordance with Scheme 16 is prepared the ethynyl substituted 2-5 azabicyclo[2.2.1]heptane starting material for compounds of Formula (I) wherein A is selected from option (e). Compound (44) (prepared according to the procedure of Stella et al., Tetrahedron Lett., a:2603 (1990)) is deprotected by hydrogenolysis over Pd/C, then reprotected by treatment with di-t-butyldicarbonate to give the BOC-protected compound (45). Compound (45) is reduced with LiAlH4 to an intermediate alcohol, which is then 10 oxidized to obtain the aldehyde (46). Compound (46) is treated with PPh3 and CBr4 to give an intermediate dibromoalkene (not shown), which is then converted to the alkyne (47) by treatment with an alkyllithium compound. Compound (47) may be substituted for compounds (1) or (6) in any of Schemes 1-13 above to give the desired compound of Formula (I).

SUBSTiTUTE SHEET (RULE 26) Scheme 17 Boc Boc I I O
N N
Lr-J~ CO,,Me 1. LiAIHq 2. Swem : H
H O- H
exo CBr4 2~
PPh3 Boc I Boc N Br n-BuLi N
H 4d~ 46 H Br broi5~~
H

In accordance with Scheme 17 is prepared the ethynyl substituted 7-azabicyclo[2.2.1Jheptane starting material for compounds of Formula (I) wherein A is selected from option (f). Compound (48) (prepared according to the procedure of Hernandez et al., J. Org. Chem., 60:2683-2691 (1995)) is reduced with LiA1H4 to an intermediate alcohol, which is then oxidized under Swern (DMSO, oxalyl chloride, NEt3) or similar conditions to obtain the aldehyde (49). Compound (49) is treated with PPh3 and CBr4 to give the dibromoalkene (50), which is then converted to the alkyne (51) by treatment with an alkyllithium compound. Compound (51) may be substituted for compounds (1) or (6) in any of Schemes 1-13 above to give the desired compound of Formula (I).
It should be noted that compounds of Formula (I) wherein R is C1-C4-alkyl, Br, Cl, F, CF3 or CC13 may be conveniently prepared by starting with the appropriately substituted compounds 2, 9, 11, 13, 13A, 15, 17, 20, 23, 26, 29, 32 or 35, which, if necessary, may be prepared by common techniques from the unsubstituted pyridine, pyrimidine or pyrazine starting materials or other commercially available derivatives thereof.
Preparation of additional iodohydroxyheterocycles may be carried out by selective electrophilic aromatic substitution reactions upon the conesponding hydroxyheterocycles. In the above selective electrophilic substitution reactions, occasionally it may be necessary or desirable to achieve the desired position of substitution by blocking a more readily substituted position with a blocking and/or directing group, e.g. chloro or nitro, which can subsequently (either prior or subsequent to cycloaddition to acetylenes, e.g. 1, 6, 38, 43, 47 and 57) be removed by, respectively, reduction or a reduction/diazotization/reduction sequence.
Alternately, a SUBSTITUTE SHEET (RULE 26) bromo- or chloro-substituent on an intermediate substituted pylidine or a fully assembled furopyridine or related heterocycle which has been constructed by way of the methods described above can be converted to other substituents. For example, by treating a compound of Formula (I) wherein R is Br with NH3, optionally with catalysis by copper salts, under heat and pressure, compounds of Formula (I) wherein R is NH2 may be prepared. Further treatment of compounds of Formula (I) wherein R is NH2 with NaNO2 and CuCN allows the preparation of compounds of Formula (I) wherein R is CN.
As a further example, amino may be oxidized with H2SO4 and H202 to nitro, or carboxamide may be dehydrated to cyano. Cyano groups may be treated with the appropriate alcohol in the presence of a strong acid to prepare compounds of Formula (I) wherein R is COO-CI-C4-alkyl. Further hydrolysis of these esters with mild base gives the compounds of Formula (I) wherein R is COOH. Or compounds of Formula (I) wherein R is NH2 may be N-acylated by the appropriate C1-C4-acyl chloride (or acyl halides selected from CI-Cg-acyl halide, substituted C1Cg-acyl halide, phenyl acyl halide, substituted phenyl-acyl-halide, heteroaryl-acyl halide, substituted heteroaryl-acyl-halide, phenyl-C1-C(-acyl halide, substituted phenyl-C 1-C6-acyl halide, heteroaryl-acyl halide, substituted heteroaryl-acyl halide or (-O-CI-C(alkyl)acyl halide to give compounds of Formula (I) wherein R is NH-CO-Cl-C4-alkyl or the acyl derivatives of the groups specified directly above.
Further, compounds of Formula (I) wherein R is NH2 may by alkylated or arylated to give the compounds of Formula (I) wherein R is NR1R1 or NR6R7. Also, bromo- or chloro-substituted compounds may be replaced with alkyl or alkenyl in reactions moderated by transition metals, e.g., palladium or nickel. Such alternate procedures as may be required are well known to those skilled in the art, and such alternate substituents are considered to be within the scope of the invention. Appropriate precursors to compounds 13, 13A, 15, 17, 29, 32 and 35 may also be prepared by ring-closure reactions of appropriately substituted acyclic compounds, such reactions being well known to those skilled in the art.
In addition to the syntheses and synthetic schemes described above, the schemes and discussion presented below are directed to preparation of compounds of formula I wherein R, when substituted at the Y2 position, may be selected from:
NR3R4, wherein R3 is H or C1-C3 alkyl and R4 is hydrogen, C1-Cg-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted -phenyl-C 1 -C6-alkyl -, heteroaryl-Cl-C6-alkyl-, and substituted-heteroaryl-C 1-C6-alkyl-;
C(O)-R5= where R5 is hydrogen, C1-C8-alkyl, substituted-C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-allcyl-, heteroaryl-Cj-C6-alkyl-, substituted-heteroaryl-Cl-C6-alkyl-, and O-C1-C6-alkyl-, N-R6R7, wherein R6 is selected SUBSTITUTE SHEET (RULE 26) from the group consisting of H and C1-C3-alkyl-, and R7 is selected from the group consisting of H, C1-C3-alkyl-, phenyl and substituted-phenyl;
OR8, wherein R8 is C1-C8-alkyl, phenyl, substituted-phenyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-Ci-C6-alkyl-, heteroaryl-Ci-C6-alkyl-, CONR3R4;
phenyl;
naphthyl;
substituted-phenyl;
substituted-naphthyl;
biphenyl;
substituted-biphenyl;
heteroaryl;
substituted-heteroaryl;
phenyl-C l-C6-alkyl-;
substituted-phenyl-C1-C6-alkyl-;
heteroaryl-C1-C6-alkyl-; and substituted-heteroaryi-C 1-C6-alkyl-;
Rg, wherein R9 is selected from the group consisting of hydrogen, Cl-Cg-alkyl, substituted-C1-Cg-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C 1-C6-alkyl-, heteroaryl-C 1-C6-alkyl-, and substituted-heteroaryl-C 1-C6-alkyl-;

Rs m , wherein m is 1 or 2, and R9 is as defined above;
-CH2-NH-CO-R5, wherein R5 is as defined above; and -CH2-CH2-CO-O-C j-C6-alkyl.
In addition to the groups or variables recited for a compound of formula I in the preceding section with respect to A, R1, R2 and R, in a compound of formula I, Rl may additionally be selected from allyl; R2 for n is 1, 2 or 3 is selected from all the variables previously specified as well as Br, Cl, F, OH, CN, -O-CO-CH3 and -0-methanesulfonyl provided said specifically referred to R2 groups are not alpha to the ring nitrogen atom in group A; R at any position is additionally selected from vinyl and hydroxy.
Treatment of compounds of formula (I) wherein R is Br with alkoxides, e.g.
those derived from C I-C6 alcohols or benzyl alcohol, for example, at elevated temperature allows preparation of of compounds of Formula (I) wherein R is CI-C6 alkyl-O- or benzyl-O-.
Removal of the benzyl group by catalytic hydrogenolysis or treatment with acid, for example, hydrogen bromide in acetic acid, provides compound of formula (I) wherein R is SUBSTITUTE SHEET (RULE 26) hydroxy. The hydroxy group can then be alkylated by appropriate halides or sulfonates to afford compounds or formula (I) wherein R is CI-C8 alkyl-O-, phenyl-C1-C6-allcyl-O-, substituted phenyl Cl-C6-alkyl-O-, heteroaryl-C1-C6-alkyl-O-, substituted heteroaryl-Cl-C6-alkyl-O-, or acylated by appropriate isocyanates or carbamoyl chlorides to afford compounds of Formula (I) wherein R is OC(O)-NR3R4. The precursor wherein R is hydroxy can alternately be prepared from the compound wherein R is amino by treatment with sodium nitrite followed by heating under acid conditions. It will apparent to those skilled in the art that some of the above transformations will be best carried out following protection of moieties elsewhere in the molecule that may be susceptible to the reaction conditions with a suitable protecting group, followed by removal of the protecting group when no longer needed by conditions well known in the art.
The compounds and processes of the present invention will be better understood in connection with the following synthetic schemes which illustrate the methods by which the compounds of the invention may be prepared. The groups n, R 1 are as defined above unless otherwise noted.

Scheme 18 (CH2)n (CH2)n Br = 0 N H O
R' + I -~- N Br I N L Ri N-~
1a 2a 3a L
In accordance with Scheme 18 intermediate compounds are prepared by reaction of an acetylene compound (la), wherein n is 1 to 3 and R1 is allyl or C1-C6-alkyl or a protecting group such as t-BOC or CBZ, for example, with a 2-iodo-3-hydroxypyridine compound (2a), wherein L is H, F or Cl or R as defined herein for compounds of Formula (I) above, in the presence of Pd, Cul and triethylamine at the elevated temperature (See Kundu, et al., J. Chem. Soc. Chem. Comm., 1992: 41 for analogous preparation of benzofurans) to form the 6-bromofuro[3,2-b]pyridyl compound (3a).

SUBSTITUTE SHEET (RULE 26) Scheme 19 (CH2)n 3a + 11~ Rs < N O
Rs 1 ...
4a 5a N
L
(CH2)n 3a + Rs N s Ri N..= R
6a 7a L
(CH2)n < N 0 R9 3a +
Rs R' N
8a 9a L

5 In accordance with Scheme 19 an intermediate compound (3a), wherein R1 is allyl or C1-C6-alkyl or a protecting group such as t-BOC or CBZ, for example, is reacted with an unsaturated compound (4a), (6a) or (8a), to give compounds (5a), (7a) or (9a), respectively, which are specific or protected compounds of Formula (I), by treatment with a palladium (II) catalyst under weakly basic conditions at reflux temperature in an organic or aqueous 10 solvent. Compound (4a) may be prepared by reacting a compound R9-CHO, wherein R9 is selected from the group consisting of hydrogen, C1-C8-alkyl, substituted-C1-Cg-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C 1-C6-alkyl-, substituted-phenyl-C 1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, and substituted-heteroaryl-C1-C6-alkyl-, with (phenyl)3P=CH2 in is refluxing toluene. Of course, these compounds may further be treated with a reducing agent (e.g. hydrogen/catalyst) to form the saturated derivatives (R= heteroarylCl-C6alkyl.
Compound (6a) may be prepared by reacting a compound R9-CHO, wherein R9 is as described above, with (Ph)3P=CH-CHO in refluxing toluene to give R9-CH=CH-CHO, then reacting R9-CH=CH-CHO with (Ph)3P=CH2 in refluxing toluene. Compound (8a) 20 may be prepared by reacting R9-CHO, wherein R9 is as described above, with CBr4 and P(Ph)3 to give R9-CH=CBr2, then reacting R9-CH=CBr2 with 2 equivalents of n-butyllithium followed by treatment with H+. In the cases wherein R1 is a protecting group such as t-BOC or CBZ it must be removed under well-known standard conditions for SUBSTITUTE SHEET (RULE 26) removing those groups in order to give the desired compound of Formula (I). In some cases wherein R1 is allyl or C1-C6-alkyl, it may be desirable to place this grouping in the compound after the protecting R1 group has been removed. When R1 is allyl, this may be accomplished by reacting the unprotected nitrogen atom with allyl chloride in the presence of a weak base such as triethylamine. When Rl is CI-C6-alkyl, this may be accomplished by reacting the unprotected nitrogen atom with the appropriate aldehyde in the presence of NaCNBH3, for example. L is chosen from the groups defined under R. Any of the above compounds may be further reduced to form a compound of the invention.

Scheme 20 (CH2)n 3a + W-B(OH)2 N
I w R
10a 11a N
L
In accordance with Scheme 20 an intermediate compound (3a), wherein R1 is allyl or C1-C6-alkyl or a protecting group such as t-BOC or CBZ, for example, is reacted with a is suitable boronic acid compound (10a) wherein W is selected from the group consisting of:
(a) phenyl; (b) naphthyl; (c) substituted-phenyl, as defined herein; (d) substituted-naphthyl, as defined herein; (e) biphenyl; (f) substituted-biphenyl, as defined herein; (g) heteroaryl, as defined herein; (h) substituted-heteroaryl, as defined herein; (i) phenyl-C1-C6-alkyl-, as defined herein; (j) substituted -phenyl-C 1 -C6-alkyl-, as defined herein; (k) -heteroaryl-C1-C6-alkyl-, as defined herein; and (1) substituted-heteroaryl-C1-C6-alkyl-, as defined herein; for Formula (I) above, in the presence of Pd(O) under the conditions of the Suzuki reaction, for example in the presence of a weak base such as NaHCO3 and in an aprotic solvent, such as toluene, benzene or methylene chloride at reflux temperatures to give a compound (1 la), wherein R3 is as described above, to produce specific compounds of Formula (I). In an alternate method, compound (l0a) may be replaced by a WMgX compound and reacted in the presence of Ni(dppp)2C12 to give compound (11). In the cases wherein R 1 is a protecting group such as t-BOC or CBZ it must be removed under well-known standard conditions for removing those groups in order to give the desired compound of Formula (I). In some cases wherein R1 is allyl or C1-C6-alkyl, it may be desirable to place this grouping in the compound after the protecting R1 group has been removed. When Rl is allyl, this may be accomplished by reacting the unprotected nitrogen atom with allyl chloride in the presence of a weak base such as triethylamine. When R1 is C1-C6-alkyl, this may be accomplished by reacting the SUBSTITUTE SHEET (RULE 26) unprotected nitrogen atom with the appropriate aldehyde in the presence of NaCNBH3, for example. L is equal to R as defined above at the designated position.

Scheme 21 (CH2)n = O
1 1 a + Phenyl-B(OH)2 N W
~
R
12a 13a N R, (CH2)n < O

11 a + ,/^*-Sn(n-Bu)3 -~ N i\ W
Rl N
14a 15a In accordance with Scheme 21 are prepared compounds of Formula (I) wherein L
is vinyl, phenyl or substituted phenyl. Reaction of a starting material compound (1 la) wherein L is chloro with phenylboronic acid (12a) in the presence of Pd(O) under the conditions of the Suzuki reaction, for example in the presence of a weak base such as NaHCO3 and in an aprotic solvent, such as toluene, benzene or methylene chloride at reflux temperatures gives the compound 13a. Reaction of a starting material compound (l la) wherein L is chloro with vinyl-Sn(n-Bu)3 (14a) in the presence of Pd(O) under Stille reaction conditions to give the compound (15a). In the cases wherein Rl is a protecting group such as t-BOC or CBZ it must be removed under well-known standard conditions for removing those groups in order to give the desired compound of Formula (I). In some cases wherein R1 is allyl or C1 -C6-alkyl, it may be desirable to place this grouping in the compound after the protecting R1 group has been removed. When R1 is allyl, this may be accomplished by reacting the unprotected nitrogen atom with allyl chloride in the presence of a weak base such as triethylamine. When R1 is C1-C6-alkyl, this may be accomplished by reacting the unprotected nitrogen atom with the appropriate aldehyde in the presence of NaCNBH3, for example.

SUBSTITUTE SHEET (RULE 26) Scheme 22 H O Br PG-O \ E B N L N L

16a PG-O W

16a /
N L
17a H-O W
12 deprotection I
17a -N L
18a (CH2)n / (CH2)n \N \\ ' 0 R, + 18 a N w ~
R Nr 19a L

In Scheme 22 is shown an alternate process for preparing desired compounds of the invention. Whereas in Schemes 19 and 20, the heterocyclic and the pyridine moieties are first joined, and the W group is added according to Schemes 19 and 20, Scheme 22 allows for the placement of the W group before joining. Accordingly a hydroxypyridine compound is treated with the appropriate reagent, such as a trialkylsilyl or benzyl chloride, to protect the hydroxyl group with a protecting group PG, such as trialkylsilyl or benzyl, respectively, for example to give compound (16a). Compound (16a) may then be reacted with an appropriate reagent, as described in Schemes 19 and 20, to give the compound (17a) having the desired substitution at L and W. L is chosen from R as designated previously at that position on the ring and W is as defined previously. Subsequent iodination followed by deprotection by standard methods gives (18a), which is then coupled with compound (1) according to the method of Scheme 18 to give the desired compound of Formula (I).
SUBSTITUTE SHEET (RULE 26) Scheme 23 (CH2)n \ C
3a + Zn(CN)2 ----0 N CN
iI
R ~=
19a N L
1) R5-NI 1) H2, Ni 2) H3O+ 2) RS-CO-X
(CH2)n < O O (CF"12)n 1 ~ O O
<
R1 ,.. R5 N ~

R
20 a N L R 21 a N
L
In accordance with Scheme 23 are prepared additional compounds of Formula (I).
Compound (3a) is first reacted with Zn(CN)2 and tetralds(triphenylphosphine)palladium(0) under anhydrous conditions in DMF or a similar solvent at room temperature to 120 C for 12-24 hours, to give the cyano intermediate compound (19a) with L selected from R as defined above. Compound (19a) may then be reacted with a reagent R5-M, wherein R5 is as described for Formula (I) above and M is lithium or a magnesium halide moiety, under the appropriate anhydrous conditions, with cooling if necessary, for 2-8 hours or until the reaction is complete to give, followed by treatment with aqueous acid to dissociate the metal complexes and give compound (20a). Alternately, the cyano group of compound (19a) may be reduced by treatment with 1 atm of H2 in the presence of Ranefnickel at room i 5 temperature for 1-8 hours to give an intermediate amino compound. The intermediate amino compound may then be treated with a suitable acylating reagent, for example ethyl formate, an acyl chloride R5-C(=O)CI wherein R5 is, for example, CI-Cg-alkyl, substituted-C1-C8-alkyl, phenyl, substituted-phenyl, heteroaryl, substituted-heteroaryl, aryl-CI-C6-alkyl-, substituted-aryl-Cl-C6-alkyl-, heteroaryl=C1-C6-alkyl-, or substituted-heteroaryl-Cl-C6-alkyl-, a CI-Cb-alkyl dicarbonate; or is treated with an appropriate carbamylating reagent, for example KOCN or an isocyanate R7NCO
wherein R7 is selected from C1-C3alkyl, phenyl or substituted phenyl or Cl-CO-N-R6R7, for example, wherein R6 may be C1-C3-alkyl-, and R7 may be CI-C3-alkyl-, phenyl or substituted-phenyl.
~ trade-mark SUBSTITUTE SHEET (RULE 26) In Vitro Determination of Neuronal Nicotinic AcMlcholine Receptor Binding Potencies For the purpose of identifying compounds as nicotinic acetylcholinergic agents which are capable of interacting with neuronal nicotinic acetylcholine receptors in the brain, 5 a ligand-receptor binding assay was carried out as the initial screen.
Compounds of the present invention were effective at interacting with neuronal nicotinic acetylcholine receptors as assayed for their ability to displace radioligand from neuronal nicotinic acetyicholine receptors labeled with [3H]-cytisine ([3H]-CYT).

10 A. Protocol For Detennination of Nicotinic Acetylcholine Receptor Binding Potencies of Ligands Binding of [3H]-CYT to nicotinic acetylcholine receptors was accomplished using crude synaptic membrane preparations from whole rat brain (Pabreza et al., Molecular Pharmacol. , 1990, 39:9). Washed membranes were stored at -80 C prior to use.
Frozen 15 aliquots were slowly thawed and resuspended in 20 volumes of buffer (containing: 120 mM
NaCI, 5 mM KCI, 2 mM MgC12, 2 mM CaCl2 and 50 mM Tris-Cl, pH 7.4 @4 C). After centrifuging at 20,000x g for 15 minutes, the pellets were resuspended in 30 volumes of buffer. Homogenate (containing 125-150 g protein) was added to triplicate tubes containing concentrations of test compound and [3H]-CYT (1.25 nM) in a final volume of 20 500 pL. Samples were incubated for 60 minutes at 4 C, then rapidly filtered through Whatman*GFB filters presoaked in 0.5% polyethylimine using 3 x 4 mL of ice-cold buffer.
The filters were counted in 4 mL of Ecolume (ICN). Nonspecific binding was determined in the presence of 10 pM (-)-nicotine and values were expressed as a percentage of total binding. IC50 values were determined with the RS-1 (BBN) nonlinear least squares curve-25 fitting program and IC50 values were converted to Ki values using the Cheng and Prusoff correction (Ki=IC$0,/(1+[ligand]/Kd of ligand). The results (shown in Table 1) suggest that the compounds of the present invention have high affmity for the neuronal nicotinic acetylcholine receptor.

30 The ability of the compounds of the invention to interact with nicotinic acetylcholine receptors and thereby to activate or inhibit synaptic transmission can be demonstrated in vitro using the following protocol.

B. Protocols for the Detenmination of Functional Effects of Nicotinic Acetylcholine RecMtor 35 Ligands on Synaptic Transmission Cells of the IMR-32 human neuroblastoma clonal cell line (ATCC, Rockville, MD) were maintained in a log phase of growth according to established procedures (Lukas, R.J., * trade-mark SUBSTiTUTE SHEET (RULE 26) J. Pharmacol. Exp. Ther., 265: 294-302, 1993). Experimental cells were seeded at a density of 500,000 cells/mL into a 24-well tissue culture dish. Plated cells were allowed to proliferate for at least 48 hours before loading with 2 Ci/mL of 86Rb+ (35 Ci/mmol) overnight at 370C. The 86Rb+ efflux assays were performed according to previously published protocols (Lukas, 1993) except serum-free Dulbecco's Modified Eagle's Medium was used during the 86Rb+ loading, rinsing, and agonist-induced efflux steps.
Cells of the K177 cell line, resulting from stable transfection of the human embryonic kidney (HEK) 293 cell line with the cDNA of the a4 and 02 nicotinic acetylcholine subunits (Gopalakrishnan, et al, J. Pharmacol. Expt. Ther. 1996, 276, 289-297), were maintained in a log phase of growth according to established procedures (Gopalalaishnan, et al., loc. cit.). The cells were plated onto poly-lysine coated 24-well Costar plates (Cambridge, MA) at a density of 250,000 cells/well. When confluent, the cells were loaded with 86Rb+ and agonist-induced efflux was assessed as reported above for IMR-32 cells.
Maximal responses (reported as percent relative to the response elicited by 100 pM
(S)-nicotine) are shown for selected compounds of the invention. The inhibition data (given for other selected compounds) reflect inhibition of the efflux elicited by 100 pM (S)-nicotine at the indicated concentration. The results (also shown in Table 1) suggest that selected compounds of the present invention either activate or inhibit the initial ion flux aspects of synaptic transmission mediated by neuronal nicotinic acetylcholine receptors.
This finding is in agreement with the results of others who have linked dopamine release, which is dependent upon the ion flux in synaptic transmission, to binding at nicotinic receptors (cf., for example, Lippiello and Caldwell, U.S.Patent 5,242,935, issued Sept. 7, 1993; Caldwell and Lippiello, U.S.Patent 5,248,690, issued Sept. 28, 1993; and Wonnacott et al., Prog.
Brain Res., 79: 157-163 (1989)).

Table 1 Binding to Neuronal Nicotinic Acetylcholine Receptors and Activation or Inhibition of Neuronal Nicotinic Acetylcholine in K177 or IMR-32 Cells Ez.No [3]HO-Cyt K177 K177 IMR-32 IMR-32 Binding % max % Inhibition % max % Inhibition O @10uM @10uM
1 2.7 79 7 16 30u 3 4.1 34 58 4 17.5 63 6 4.7 68 14 4 SUBSTITUTE SHEET (RULE 26) 12 4.1 41 11 0.45 9 43 16 5.8 109 0 10 18 38 55 30u 21 3300 0 5 30u 23 0.62 26 0.66 6 53 7 38 28 4.8 20 29 6.8 0 28 20 0 31 9.8 13 41 39 34 1710 18 2.8 1.1 61 38 0.33 0 12 10 38 1.1 39 4 25 42 4.7 29 24 48 1.3 0 6 0 49 2.6 1 10 52 0.86 36 69 In addition to the data presented above which demonstrates that the compounds of the invention are effective binders to the nicotinic acetylcholine receptor and are functional ligands, the following table (Table 2) shows additional data for examples 53-59 and 134-s 136.

SUBSTITUTE SHEET (RULE 26) Table 2 Binding to Neuronal Nicotinic Acetylcholine Receptors and Activation of Neuronal Nicotinic Acetylcholine Receptors in IMR-32 Cells Ex. No Binding IMR-32 nM % max 55 0.16 32 56 12.9 57 0.83 22 58 0.31 134 6.9 135 3.2 136 2.4 As can be seen from Tables 1 and 2, compound 55 exhibited potent in vitro binding. The following examples will serve to further illustrate preparation of the novel compounds of the invention. They are not to be read as limiting the scope of the invention as it is defined by the appended claims.
Thin-layer chromatography (TLC) was performed on 0.25 mm E. Merck precoated silica gel plates (60 F-254). Flash chromatography was performed on 200-400 mesh silica gel (E. Merck), and column chromatography was performed on 70-230 mesh silica gel (E.
Merck).
The following abbreviations are used: THF for tetrahydrofuran, DMF for N, N-dimethylformamide, D20 for deuterium oxide, CDC13 for deuterochloroform, DMSO-d6 for deuterodimethylsulfoxide, BOC for tert-butyloxycarbonyl, CBZ for benzyloxycarbonyl, Bn for benzyl, Ms for methanesulfonyl, PAW for pyridine/acetic acid/water (20:6:11), DCC for dicyclohexylcarbodiimide, DIBALH for diisobutylaluminum hydride, DIEA for diisopropylethylamine, DPPA for diphenylphosphororyl azide, DME for 1,2-dimethoxyethane, EDC for 1-(3-dimethyl-aminopropyl)-3-ethylcarbod'umide hydrochloride, EtOH for ethanol, Et20 for diethyl ether, IBCF for isobutyl chloroformate, HOAc for acetic acid, HOBT for 1-hydroxybenzotriazole, LAH for lithium aluminum hydride, MeOH
for methanol, NH4OAc for ammonium acetate, dppp for 1,3-bis(diphenylphosphino)propane;
NMM for N-methylmorpholine, TEA for triethylamine, THF for tetrahydrofuran.
SUBSTiTUTE SHEET (RULE 26) Ex amnle 1 Preparation of 2-(1-methyl-2-(S)-pyaolidinvl)furof3.2-blpvridine dihvdrochloride 1a. N-BOC-(S)-prolinal N-BOC-(S)-proline was reduced to N-BOC-(S)-prolinol by treatment with diborane as described by K.E. Rittle et al. (J. Org. Chem., 47:3016 (1982)). N-t-butyloxycarbonyl-(S)-prolinol was then oxidized to N-t-butyloxycarbonyl-(S)-prolinal by treatment with DMSO and sulfur trioxide-pyridine complex as described by Y. Hamada and T.
Shioiri (Chem. Pharm. Bull, 5:1921 (1982)).
1 b. 2(S)-(2.2-Dibromoethenyl)-N-t-butvloxycarbonylpyrrolidine At room temperature and under nitrogen, triphenylphosphine (13.0 g, 49.5 mmol), zinc dust (2.16 g, 33.0 mmol) and carbon tetrabroniide (11.0 g, 33.0 mmol) were added to CH2Cl2 (80 mL). After stirring for 5 minutes, a solution of N-t-butyloxycarbonyl-(S)-prolinal (3.29 g, 16.5 mmol) in CH2C12 (25 mL) was added. The reaction was slightly exothermic. After stirring for 1 hour, the reaction mixture was diluted with EtOAc/hexane (1:1) and filtered through basic alumina. The filter cake was then washed with a mixture of CH2CI2/EtOAc/hexane (1:1:1). The filtrate was concentrated in vacuo, and the residue was taken up in EtOAc/hexane (1:1). The resulting precipitate was filtered, and the filtrate was concentrated. The residual oil was subjected to flash chromatography using EtOAc/hexane (1:6.5 to 1:5) as the eluant. The resultant pure solid product was isolated in 91 % yield (5.31 g): mp 65-66 C; [a]D23 +20.1 (c 1.10, MeOH); IH NMR (DMSO-d6, 70 C, 300 MHz) S 6.57 (d, J=8.1 Hz, 1H), 4.26 (ddd, J=7.9, 7.9, 4.9 Hz, 1H), 3.30 (m, 2H), 2.11 (m, IH), 1.72-1.92 (m, 2H), 1.65 (m, 1H), 1.40 (s, 9H); MS m/e 354 (M+H)+; Anal.
Calcd for C11H17Br2NO2: C, 37.21; H, 4.83; N, 3.95. Found: C, 37.45; H, 4.85; N, 3.97.
I c. 1-BOC-2-(S )-ethknylpvrrolidine A solution of the compound of step 1 b above (27.1 g, 76.3 mmol) and THF (550 mL) was cooled to -75 C. Under a nitrogen atmosphere, a 2.5 M solution of n-butyllithium in hexane (62.6 mL, 156 mmol) was added dropwise over a 15 minute period. After stirring for 1 hour, saturated aqueous sodium bicarbonate was added dropwise to the reaction flask.
The dry ice bath was removed and an additional portion of saturated aqueous sodium bicarbonate was added. The mixture was extracted with EtOAc (3X) and the combined organic phases dried over anhydrous sodium sulfate and concentrated in vacuo.
The resulting residue was purified by flash chromatography on silica gel eluting with Et20/hexane (1:6 to 1:5) to give 11.5 g (77% yield) of the title compound (1 c) as an oil:
[a]D23 -92.1 (c 2.20, MeOH); IH NMR (CDCI3, 300 MHz) S 4.45 (m, 1H), 3.53-3.24 (m, 2H), 2.25-1.85 (m, 5H), 1.48 (s, 9H); MS (CI) m/e 196 (M+H)+.

SUBSTITUTE SHEET (RULE 26) 1 d. 2-(1-BOC-2-(S)_12yrrolidinyl)furo [3.2-blpxridine A 2.34 g (12 mmol) sample of the compound from step lc above was dissolved in 15 mL of DMF, and (Ph3P)2PdC12 (0.6 mmol), Cul (0.74 mmol) and triethylamine (14.25 mmol) were added. The mixture was stirred at room temperature for 1 hour, then 3.14 g 5 (14.4 mmol) of 2-iodo-3-hydroxypyridine (Lancaster Chem. Co.) was added. The reaction mixture was st.irred at 60 OC for 16 hours. The solution was cooled, diluted with toluene, and the volatiles were removed under reduced pressure. The residue was dissolved in 1 N
HCI, and this solution was extracted with ether. The acidic aqueous layer was adjusted to pH 10 with K2C03, and this solution was extracted with CH2C12. The CH2C12 extract 10 was washed with 20% NaOH, dried over MgSO4, and evaporated. The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH to give 980 mg of title compound: 1H NMR (CDC13, 300 MHz) 6 1.32 (s, 9H), 1.90-2.20 (m, 4H), 2.95-3.15 (m, 2H), 5.05 (m, 1 H), 6.68 (s, 1H), 7.15 (br s, 1 H), 7.67 (d, 1 H, J=8 Hz), 8.48 (d, 1 H, J=3 Hz); MS m/z: 289 (M+H)+.
15 1 e. 2-(1-methyl-2-(S)-pyrrolidin 1)uro[3 2-bli2yridine dihtidrochloride A 147 mg sample of the compound from step 1 d above was dissolved in 4 mI. of HCHO and 2 mL of 88% formic acid and heated at reflux for 25 minutes. The solution was cooled, diluted with water, and adjusted to pH 10 with K2C03. The mixture was extracted with CH202, and the extract dried and concentrated. The residue was purified by 20 chromatography on silica gel, eluting with 100:0 to 90:10 CHC13:MeOH. The product was dissolved in ethanol, and a solution of HCI in Et20 was added dropwise. The resultant white precipitate was then collected by evaporation of solvent and triturated with three portions of Et20 to give the title compound (200 mg): I H NMR (CDC13, 300 MHz) S
2.35-2.37 (m, 2H), 2.58-2.67 (m, 3H), 3.00 (br s, 3H), 3.40 (br s, 1H), 3.90 (br s, 1H), 25 7.45 (s, 1H), 7.70 (dd, 1H, J=5, 8.5 Hz), 8.33 (d, 1H, J=8.5 Hz), 8.64 (dd, 1H, J=5, 1 Hz). MS m/z: 203 (M+H)+; Anal. Calcd for C12H14N2O=2.0 HCl=0.2 H20=0.1 EtOH:
C, 51.99; H, 6.08; N, 9.94. Found: C, 51.59; H, 6.03; N, 9.68.

Examnle 2 30 Preparation of 2-(2-(S)-nyrrolidinvl)furof3 2-blpyridine dihvdrochloride A 980 mg sample of 2-(1-BOC-2-(S)-pyrrolidinyl)furo[3,2-b]pyridine, from Example ld above, was dissolved in a solution of TFA in CH2C12 at 0OC and stirred under N2 while warming to room temperature. The reaction mixture was diluted with I
N HCI, 35 and the aqueous layer was separated. The aqueous solution was adjusted to pH 10 with K2C03, and the mixture was extracted with CH2C12. The solution was dried over MgSO4 and concentrated. The residue was purified by chromatography on silica gel and treated SUBSTITUTE SHEET (RULE 26) with HCl in Et20 as described in Example le to obtain 280 mg of title compound: 1H NMR
(CDC13, 300 MHz) S 2.17-2.52 (m, 3H), 2.65 (m, 1H), 3.57 (dt, 2 H, J=1.5, 7.5 Hz), 5.15 (t, 1H, J=8 Hz), 7.44 (s, 1H), 7.84 (dd, 1H, J=6, 8.5 Hz), 8.5 (dt, 1H, J=l, 8.5 Hz), 8.70 (dd, 1H, J= 1, 6 Hz). MS m/z: 189 (M+H)+, 206 (M+NH4)+; Anal. Calcd for C I I H 12N20=2.0 HC1: C, 50.59; H, 5.40; N, 10.73. Found: C, 50.52; H, 5.26;
N, 10.50.
Example 3 Preparation of 2-(2-(R)-pvrrolidinvl)furof3.2-blQvridine dihydrochloride 3a. 1-BOC-2-(R)-eth~n,vluvrrolidine The title compound was prepared from N-BOC-(R)-proline according to the procedures of Examples la-c above. [a]D23 +113.0 (c 0.94, MeOH).
3b. 2-(1-BOC-2-(R)-pyrrolidinyl)furof 3.2-blpvridine A 3.14 g (14.4 mmol) sample of 2-iodo-3-hydroxypyridine (Lancaster Chem. Co.) was dissolved in 5 mL of DMF, and (Ph3P)2PdC12 (0.34 g, 0.50 mmol), Cul (0.371 g, 1.98 mmol) and triethylamine (1.80 mL, 13.2 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 2.15 g (11.0 mmol) of the compound from step 3a above, dissolved in 5 mL of DMF, was added carefully. The mixture was stirted at 60 OC for 16 hours, then cooled to room temperature. The reaction mixture was diluted with ether and filtered. The solution was washed with 10% NaOH, 50% brine, dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 100:0 to 60:40 hexane:EtOAc to give 620 mg of title compound: IH
NMR
(CDC13, 300 MHz) S 1.73 (s, 9H), 1.85-2.30 (m, 4H), 3.05-3.22 (m, 2H), 4.42 (m, 1 H), 6.78 (s, 1H), 7.16 (dd, 1H), 7.68 (dd, 1H), 8.48 (dd, 1H); MS m/z: 289 (M+H)+.
3c. 2-(2-(R)-nyrrolidinyl)furof3.2-b]pyridine dihydrochloride A 614 mg (2.13 mmol) sample of 2-(1-BOC-2-(R)-pyrrolidinyl)furo[3,2-b]pyridine, from step 3b above, was dissolved in 3 mL of CH2CI2, and the solution was cooled to 0 0 C. To this solution was added 3 mL of TFA, and the reaction mixture was stirred at 0OC
for 2 hours. The reaction was quenched with saturated aqueous K2C03 solution, and the mixture was extracted with CH2Cl2. The organic extract was dried over MgSO4, and the solvent was removed. The residue was purified by chromatography on silica gel and treated with HCl in Et20 as described in Example le above to obtain the title compound: 1H NMR
(D20, 300 MHz) 6 2.17-2.69 (m, 4H), 3.52-3.59 (m, 2 H), 5.14 (t, 1H, J=5.5 Hz), 7.42 (t, 1 H, J=1 Hz), 7.80 (dd, 1 H, J=5.6, 8.5 Hz), 8.5 (dt, 1 H, J=1, 8.5 Hz), 8.70 (dd, 1 H, J= 1, 5.5 Hz); MS m/z: 189 (M+H)+, 206 (M+NH4)+; Anal. Calcd for Cl JH12N2O=2.0 HC1=0.5 H20: C, 48.90; H, 5.60; N, 10.90. Found: C, 48.75; H, 5.74; N, 10.11.

SUBSTITUTE SHEET (RULE 26) Examlile 4 Preparation 2-(1-methvl-2-(R)-pnolidinyl)furof 3.2-blpvridine dihvdrochloride A 140 mg sample of the compound from Example 3 above was dissolved in 8 mL of 37% aqueous formaldehyde and 4 mL of 88% formic acid and heated at reflux for 1 hour.
The solution was poured into saturated aqueous K2C03 solution, and the mixture was extracted with CH2C12. The organic extract was dried, concentrated and purified by chromatography on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH. The product was dissolved in ethanol, and a solution of HCl in Et20 was added dropwise at ambient temperature. The resultant white precipitate was then collected by evaporation of solvent and triturated with three portions of Et20 to give the title compound (60 mg): IH
NMR (D20, 300 MHz) S 2.35 (br, 2H), 2.53-2.70 (m, 3H), 3.00 (br s, 3H), 3.40 (br s, 1H), 3. 90 (br s, 1 H), 7.38 (s, 1 H), 7.60 (dd, 1 H), 8.33 (d, 1 H), 8.64 (dd, 1H); MS m/z:
203 (M+H)+, 220 (M+NH4)+; Anal. Calcd for C12H14N20=2.0 HCl= 1.0 H20: C, 49.16; H, 6.19;
N, 9.55. Found: C, 49.03; H, 6.08; N, 9.13.
ExamQlp, 5 Preparation 5-methyl-2-(2-(S)-pvrrolidinyl)furof3.2-blpvridine dihydrochloride 5a. 2-(1-BOC-2-(S)-Ayrrolidinyl)-5-methvlfurof3.2-blpvridine A 3.10 g (13.2 mmol) sample of 6-iodo-2-picoline-5-ol (Aldrich Chem. Co.) was dissolved in 5 mL of DMF, and (Ph3P)2PdCl2 (0.38 g, 0.50 mmol), Cul (0.377 g, 1.98 mmol) and triethylamine (1.80 mL, 13.2 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 2.15 g (11 mmol) of 1-BOC-2-(S)-2s ethynylpyrrolidine, from Example ic above, dissolved in I mL of DMF, was added carefully. The reaction was stirred at 60 OC for 16 hours, then cooled to room temperature.
The reaction mixture was diluted with 2 N HCl and extracted with ether. The aqueous layer was adjusted to pH 10 with K2C03, then extracted with CH2C12. The extract was washed with 20% NaOH, brine, dried over MgSO4, and the solvent was removed. The residue was repeatedly dissolved in toluene and distilled to azeotropically remove the DMF. The residue was chromatographed on silica gel, eluting with 100:0 to 50:50 hexane:EtOAc to give 521 mg of title compound: 1H NMR (CDC13, 300 MHz) S 1.33 and 1.47 (2 s, 9H), 1.90-2.30 (m, 4H), 2.63 (s, 3H), 3.45-3.65 (m, 2H), 4.95 and 5.10 (2 s, 1H), 5.58 (s, IH), 7.02 (d, 1H), 7.55 (d, 1H); MS m/z: 303 (M+H)+.
5b 5-methyl-2-(2-(S)-pvrrolidiny, )1 furoL3 2-blpyridine dihydrochloride To a 530 mg sample of the compound from step 5a above in 4 mL of CH2C12 at 0 0 C was added 4 ml. of TFA. The reaction nuxture was stirred for 16 hours, then diluted with SUBSTITUTE SHEET (RULE 26) saturated aqueous Na2CO3, and extracted with CH202. The organic extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 100:0 to 90:10 CHC13:EtOH. The residue was treated with HCI/ether, and the salt was recrystallized from ethanol/EtOAc to give 158 mg of title compound: 1 H NMR
(DMSO, 300 MHz) S 2.0-2.5 (m, 4H), 2.55 (s, 3H), 3.34 (m, 3H), 4.93 (m, 1H), 7.24 (s, 1H), 7.27 (d, 1H), 7.97 (d, 1H); MS m/z: 203 (M+H)+, 220 (M+NH4)+; Anal. Calcd for C12H14N20=2.0 HCl=0.5 H20: C, 50.72; H, 6.03; N, 9.86. Found: C, 50.53; H, 6.06; N, 9.62.

Examnle 6 Preparation of 2-(1-methyl-2-(S)-nyrrolidinvl)-5-methylfuro[3,2-blpyridine dihydrochloride A 315 mg (1.04 mmol) sample of 2-(2-(S)-pyrrolidinyl)-5-methylfuro[3,2-b]pyridine dihydrochloride, from Example 5b above, was dissolved in 5 mL of 88% formic acid and 10 mL of 37% aqueous formaidehyde and heated at reflux for 0.5 hours.
The reaction mixture was cooled, diluted with 2 N HCl and extracted with ether.
The aqueous solution was adjusted to pH 10 with K2C03 and extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:EtOH The residue was converted to the salt by treatment with HCI/ether, and the salt was recrystallized from EtOH/EtOAc to give 332 mg of title compound: IH NMR (DMSO, 300 MHz) S 2.13-2.23 (m, 2H), 2.35-2.60 (m, 3H), 2.71 (s, 3H), 2.88 (s, 2H), 3.33 (br s, 1H), 3.70 (br s, IH), 4.88 (m, 1 H), 7.54 (d, IH, J=8.8 Hz), 7.61 (s, 1 H), 8.36 (d, 1 H, J=8.5 Hz);
MS m/z:
217 (M+H)+, 234 (M+NH4)+; Anal. Calcd for C13H16N20=2.0 HCl=1.0 H20: C, 50.82;
H, 6.55; N, 9.12. Found: C, 50.47; H, 6.77; N, 8.92.
ExamQle 7 Preparation of 6-chloro-2-(2-(S)-pvrrolidinyl)-furol3.2-blpyridine dihvdrochloride 7a. 5-chloro-2-iodo-3-pvridinol A 20.3 g(0.157 mol) sample of 5-chloro-3-pyridinol (Aldrich Chemical Co.) and g (0.33 mol) of Na2CO3 were dissolved in 220 mL of H20. To this solution was added 39.9 g of 12, and the reaction mixture was stirred for 45 minutes. The mixture was then poured slowly into 2 N HCI, and the acidity was adjusted to pH 3. The product was collected by filtration and crystallized from EtOH/ether, affording 23.35 g of title compound:
SUBSTITUTE SHEET (RULE 26) 1H NMR (CDC13, 300 MHz) 6 5.45 (s, 1H), 8.0 (d, 1H); MS m/z: 256 (M+H)+, 273 (M+NH4)+
7b. 2- 1-BOC-2-(S)-yyrrolidinyll-6-chlorofuro[3.2-b]pyridine A 5.63 g (22.0 mmol) sample of 5-chloro-2-iodo-3-pyridinol, from step 7a above, was dissolved in 10 mL. of DMF, and (Ph3P)2PdC12 (0.38 g, 0.50 mmol), Cul (0.377 g, 1.98 mmol) and triethylamine (1.90 mL, 13.6 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 2.15 g (11.0 mmol) of 1-BOC-2-(S)-ethynylpyrrolidine, from Example lc above, dissolved in 5 mL of DMF, was added carefully. The reaction was stirred at 60 OC for 16 hours, then cooled to room temperature.
The reaction mixture was diluted with ether, then washed with 10% NaOH and brine, then dried over MgSO4. The solvent was removed, and the residue was chromatographed on silica gel, eluting with 100:0 to 60:40 hexane:EtOAc to give 2.04 g of title compound: 1H
NMR (CDC13, 300 MHz) S 1.3, 1.45 (2 s, 9H), 1.94-2.3 (m, 4H), 3.45-3.65 (m, 2H), 4.97-5.1 (m, 1H), 6.66 (s, 1H), 7.70 (s, 1H), 8.47 (s, 1H); MS m/z: 323 (M+H)+.
7c. 6-chloro-2-(2-(S)-12yrrolidinyl)-furoi3.2-blpvridine dihvdrochloride To a 2 g sample of the compound from step 7b above in 10 mL of CH2C12 at 0OC
was added 10 mL of TFA, and the reaction mixture was stirred for 1 hour, poured into saturated aqueous Na2CO3 and extracted with CH2C12. The organic extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, 2o eluting with 99:1 to 95:5 CHC13:MeOH. The product was treated with HCI/ether, and the salt was recrystallized from EtOH/EtOAc to give 1.2 g of title compound: IH
NMR (D20, 300 MHz) S 2.18-2.50 (m, 3H), 2.54-2.65 (m, 1H), 3.51-3.36 (m, 2H), 5.06 (t, 1H, J=8 Hz), 7.26 (d, 1H, J=0.7 Hz), 8.24 (dd, 1H, J=0.7, 1.8 Hz), 8.60 (d, 1H, J=1.8 Hz); MS
m/z: 223 (M+H)+, 240 (M+NH4)+; Anal. Calcd for C11H11N20C1=2.0 HCI: C, 44.69;
H, 4.43; N, 9.47. Found: C, 44.57; H, 4.31; N, 9.33.

Fxamnle 8 Preparation of 6-chloro-2-(1-methyl-2-(S)-pvrrolidinyl)-furof 3.2-b]pvridine dihvdrochloride A 315 mg (1.04 mmol) sample of 6-chloro-2-(2-(S)-pyrrolidinyl)-furo[3,2-b]pyridine dihydrochloride, from Example 7c above, was dissolved in 3 mL of 88% formic acid and 6 mL of 37% aqueous formaldehyde and heated at reflux for 0.5 hour.
The reaction mixture was cooled, poured into saturated K2C03, and the mixture was extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed.
The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH. The SUBSTiTUTE SHEET (RULE 26) residue was converted to the salt by treatment with HCl/ether, and the salt was recrystallized from ethanol/EtOAc to give 159 mg of title compound: 1H NMR (D20, 300 MHz) 8 2.31-2.39 (m, 2H), 2.52-2.70 (m, 3H), 2.96 (br s, 3H), 3.55 (br s, 1H), 3.88 (br s, 1H), 7.33 (s, IH), 8.13 (dd, 1 H), 8.56 (d, IH); MS m/z: 237 (M+H)+, 254 (M+NH4)+; Anal.
5 Calcd for C12H13N20C1=2HC1: C, 46.55; H, 4.88; N, 9.05. Found: C, 50.75; H, 5.12; N, 9.69.

Examille 9 Preparation of 2-(2-(S)-Ryrrolidinyl)furof2.3-clpyridine dihydrochloride 9a. 4-iodo-3-methoxvmethoxYg,vridine The title compound was prepared according to the procedure of Winkle and Ronald, J. Org. Chem., 47:2101-2106 (1982).
9b. 3-h, droxy-4-iodoQ ridine A 1.48 g (5.3 mmol) sample of 4-iodo-3-methoxymethoxypyridine, from step 9a above, was suspended in 10 mL of 50% aqueous acetic acid and 4 drops of concentrated H2SO4, and the mixture was heated at reflux for 20 minutes. The solution was cooled, adjusted to pH 3 with solid Na2CO3, diluted with water, and extracted with EtOAc. The organic extract was dried over MgSO4, and the solvent was removed to give 0.86 g of the title compound: MS m/z: 223 (M+H)+, 239 (M+NI34)+.
9c. 2-(1-BOC-2-(S)-pyrrolidinyl)furo[2.3-c]pxridine A 829 mg (3.7 mmol) sample of 3-hydroxy-4-iodopyridine, from step 9b above, 130 mg (0.18 mmol) of (Ph3P)2PdCl2, 170 mg (0.74 mmol) of Cul, and 0.6 mL of triethylamine were combined in 10 mL, of DMF at ambient temperature and stirred for 3 hours. To this mixture was added a solution of 1-BOC-2-(S)-ethynylpyrroiidine (1.5 g, 7.7 mmol, from Example lc above) in 5 mL of DMF, and the reaction mixture was stirred at 60 0 C for 16 hours. The reaction mixture was cooled to room temperature and diluted with ether. The ether layer was filtered, washed with 10% NaOH then 50% brine, dried over MgSO4, and concentrated. The residue was purified by chromatography on silica gel, eluting with 100:0 to 50:50 hexane:EtOAc to give the title compound.
9d. 2-(2-(S )-pyrrolidinyl)furof 2.3-clpyridine dihydrochloride To a 700 mg sample of the compound from step 9c above in 5 mL of CH2C12 at 0 0 C was added 5 mL of TFA. The reaction mixture was stirred for 1 hour at 0OC
then poured into saturated Na2CO3, and the layers were separated. The aqueous layer was extracted with CH2C12. The combined organic layers were dried over MgSO4 and concentrated, and the residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH.
The product was converted to the salt by treatment with HCl/ether, which was recrystallized SUBSTITUTE SHEET (RULE 26) from ethanol/EtOAc: 1 H NMR (D20, 300 MHz) S 2.19-2.52 (m, 3H), 2.64 (m, 1H), 3.53-3.58 (m, 2H), 5.13 (t, 1H, J=8 Hz), 7.34 (s, 1H), 8.05 (dd, 1H, J=0.8, 5.8 Hz), 8.49 (d, 1H, J=5.8 Hz), 9.07 (s, 1H); MS m/z: 189 (M+H)+, 206 (M+NH4)+; Anal.
Calcd for C11H12N20=2.0 HCl=H20: C, 47.33; H, 5.78; N, 10.03. Found: C, 47.32;
H, 5.83; N, 9.90.

Fx amole 10 Preparation of 2-(1-methvl-2-(S)-pvrrolidinvl)furof2.3-clpyridine dihydrochloride A 120 mg sample of the compound from Example 9 above was dissolved in 4 mL of formic acid and 2 mL of formalin, and the reaction mixture was heated at reflux for 30 minutes. The reaction mixture was cooled to ambient temperature and poured into saturated K2C03 solution. The resulting mixture was extracted with CH2C12, the extract was dried, and the solvent was removed. The residue was chromatographed on silica gel, and the is compound was converted to the salt by treatment with HCl/ether: 1 H NMR
(D20, 300 MHz) 6 2.30-2.40 (m, 3H), 2.50-2.74 (m, 1H), 2.98 (s, 3H), 3.45 (br d, 1H), 3.85 (br s, 1H), 4.97 (t, 1H), 7.47 (s, IH), 8.08 (d, 1H), 8.51 (d, 1H), 9.10 (s, 1H); MS
m/z: 203 (M+H)+; Anal. Calcd for C12H14N20=2 HCl=0.5H20: C, 50.70; H, 6.03; N, 9.86.
Found: C, 50.69; H, 6.09; N, 9.61.
Exam{lle 11 Preparation of 5-chloro-2-(2-(S)-12,vrrolidinyl)-furof3.2-b]pvridine hydrochloride 11 a. 5-acetoxv-2-chlorop ivr dine To a solution of 5-aniino-2-chloropyridine (40.0 g, 0.311 mol) in 180 mL of 3:1 1,2-dimethoxyethane/CH2C12 at -10 C was slowly added boron trifluoride diethyl etherate (76.5 mL, 0.662 mol). Then a solution of tert-butyl nitrite (44.4 mL, 0.373 mol) in 40 mL
of 1,2-dimethoxyethane was slowly added over 15 min such that the reaction temperature remained below -5 C. The mixture was stirred for 10 min at -10 C then warmed to 0 C
and stirred for an additiona130 min. Pentane was added and the solid was collected by suction filtration (cold pentane wash) to afford 69.1 g of the tetrafluoroborate diazonium salt. This was dissolved in 350 mL of acetic anhydride, warmed to 75 C (N2 evolution) and stiured for 3 h. The volatiles were removed in vacuo and the dark residue was diluted with Et20 and washed with saturated aqueous NaHCO3. The aqueous phase was extracted with Et20. The combined ethereal extracts were washed with brine, dried (MgSO4), and concentrated. Purification by chromatography (silica gel; hexane/EtOAc 90:10 to 70:30) SU8STITUTE SHEET (RULE 26) afforded the title compound as a white solid (29.4 g, 55%): mp 45 C; 1H NMR
(CDC13, 300 MHz) S 2.35 (s, 3H) 7.35 (d, J=8.5 Hz, 1H), 7.48 (dd, J=2.9, 8.5 Hz, 1H), 8.21 (d, J=2.9 Hz, 1H); MS (CI/NH3) m/z: 172, 174 (M+H)+; 189, 191 (M+NH4)+.
11 b. 2-chloro-5-hydroxypyridine 5-Acetoxy-2-chloropyridine (11.1 g, 64.7 mmol) from step l la was dissolved in MeOH at ambient temperature and solid potassium carbonate (4.47 g, 32.4 mmol) was added. After stirring for 2 h, the volatiles were removed in vacuo and the residue was diluted with Et20 and H20. The aqueous phase was neutralized to pH 7 by the addition of 1 N aqueous HCI. The layers were separated and the aqueous phase was extracted twice with Et20. The combined organic extracts were dried (MgSO4) and concentrated to provide the title compound as a white solid (8.03 g, 96%): mp 155 C; 1H NMR (CD3OD, 300 MHz) S
7.20-7.28 (m, 2H), 7.88 (m, IH); MS (CI/NH3) m/z: 130,132 (M+H)+; 147,149 (M+NH4)+.
11 c. 6-chloro-2-iodo-3-12vridinol To a solution of 2-chloro-5-hydroxypyridine (5 g, from step I l b) and 8.6 g of Na2CO3 in 100 mL of water was added 9.8 g of 12. The mixture was stirred until the iodine color disappeared. The reaction mixture was then adjusted to pH 5 and extracted with EtOAc. The extract was dried over MgSO4, and the solvent was removed. The residue was recrystallized from MeOH to afford 5.4 g of the title compound: 1 H NMR
(CD3OD, 300 MHz) S 7.09 (d, IH, J=8.5 Hz), 7.20 (d, IH, J=8.5 Hz); MS m/z: 256 (M+H)+, 273 (M+NH4)+.
l id. 2-(1-BOC-2-(S)-pynolidinyl)-5-chlorofuro(3.2-b1py ridine A 3.07 g (12.0 mmol) sample of 6-chloro-2-iodo-3-pyridinol, from step l lc above, was dissolved in 10 mL, of DMF, and (Ph3P)2PdCI2 (0.38 g, 0.50 mmol), Cul (0.380 g, 1.98 mmol) and triethylamine (1.7 mL, 12 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 1.95 g (10.0 mmol) of 1-BOC-2-(S)-ethynylpyrrolidine, from Example 1a above, dissolved in 5 mL of DMF, was added carefully. The reaction mixture was stirred at 60 C for 16 hours, cooled to room temperature, diluted with ether, washed with 50% brine and dried over MgSO4, then the solvent was removed. The residue was chromatographed on silica gel, eluting with 100:0 to 50:50 hexane:EtOAc to give 1.54 g of title compound: 1H NMR (DMSO, 300 MHz, C) 8 1.37 (two s, 9H), 1.89-2.07 (m, 3H), 2.37 (m, 1H), 3.40-3.54 (m, 1H), 4.98 (m, 1H), 6.72 (s, 1H), 7.26-7.29 (d, 1H, J=8.6 Hz), 7.93-7.96 (d, 1H, J=8.6 Hz);
MS n-ilz:
323 (M+H)+.

SUBSTiTUTE SHEET (RULE 26) 11 e. 5-chloro-2-(2-(S)-12rrolidinyl)-furo[3.2-blpvridine hydrochloride A 1.5 g sample of the compound from step 1 ld above was dissolved in 10 mL of CH202 and cooled to 0"C. The solution was stirred under N2, 10 mL of TFA was added, and the reaction mixture was stirred for 1 hr. The reaction mixture was poured into saturated K2C03, and the mixture was extracted with CH2C12. The solution was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 99:1 to 95:5 CHC13:MeOH. The residue was converted to the salt by treatment with HCl/ether to give 0.78 g of title compound: IH NMR (D20, 300 MHz) S 2.22-2.65 (m, 4H), 3.52-3.57 (m, 2H), 5.06 (t, 1 H, J=8 Hz), 7.15 (d, 1 H), 7.48 (d, 1 H, J=8.6 Hz), 8.1 (d, 1H, J=8.6 Hz); MS m/z: 223 (M+H)+, 240 (M+NH4)+; Anal. Calcd for CI 1 H I 1 N20C1= 1.0 HCI: C, 50.99; H, 4.67 N, 10.81. Found: C, 51.21; H, 4.79; N, 10.55.

Examnle 12 Preparation of 5-chloro-2-(1-methvl-2-(S)-p, rr olidinvl)-furo[3.2-b]pvridine hydrochloride A 660 mg (1.04 mmol) sample of 2-(2-(S)-pyrrolidinyl)-5-chlorofuro[3,2-b]pyridine dihydrochioride, from Example l le above, was dissolved in 5 mL of 88%
formic acid and 10 mL of 37% aqueous formaldehyde and heated at reflux for 1 hour. The reaction mixture was cooled, poured into saturated K2C03, and the mixture was extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed.
The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH. The residue was converted to the salt by treatment with HCI/ether to give 540 mg of title compound: IH NMR (D20, 300 MHz) S 2.28-2.38 (m, 2H), 2.49-2.72 (m, 2H), 2.92 (br s, 3H), 3.41 (m, IH), 3.80 (m, 1H), 4.84 (m, IH), 7.50 (d, 1H, J=8.8 Hz), 8.0 (d, 1H, J=8.8 Hz), 8.56 (d, 1H); MS m/z:.237 (M+H)+; Anal. Calcd for C12H13N20C1=1.0 HCI:
C, 52.07; H, 5.13; N, 10.12. Found: C, 51.85; H, 5.46; N, 9.78.

F.xample 13 Preparation of 5-chloro-2-(2-(S)-jpyrrolidinyl)-furof2 3-blRy-idine hydrochloride 13a. 5-chloro-3-iodo-2-p,vridinol A 6.48 g sample of 5-chloro-2-pyridinol (Aldrich) and 10.8 g of Na2CO3 were dissolved in 250 mL of water. To this solution was added 12.73 g of 12, and the mixture was stirred until the iodine color disappeared. The reaction mixture was then adjusted to pH
7 and extracted with EtOAc. The extract was dried over MgSO4, and the solvent was removed. The residue was recrystallized from ethanol/water to afford 4 g of the title SUBSTITUTE SHEET (RULE 26) compound: 1H NMR (DMSO-d6, 300 MHz) 8 7.71 (d,1H), 8.18 (d, 1H); MS m/z: 256 (M+H)+, 273 (M+NH4)+.
13b. 2-(1-BOC-2-(S)-pyrrolidinvl)-5-chlorofurof 2,3-blpyridine A 3.07 g (12 mmol) sample of 5-chloro-3-iodo-2-pyridinol, from step 13a above, was dissolved in 10 mL of DMF, and (Ph3P)2PdC12 (0.39 g, 0.5 mmol), CuI (0.38 g, 1.98 mmol) and triethylamine (1.7 mL, 12 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 1.95 g (10 mmol) of 1-BOC-2-(S)-ethynylpyrrolidine, from Example ic above, dissolved in 5 mL of DMF, was added carefully. The reaction mixture was stirred at 60 OC for 16 hours, cooled to room temperature, diluted with ether and washed with 50% brine. The organic layer was dried over MgSO4 and concentrated.
The residue was chromatographed on silica gel, eluting with 100:0 to 50:50 hexane:EtOAc to give 1.55 g of title compound: MS m/z: 323 (M+H)+.
13c. 5-chloro-2-(2-(S)-pyrrolidinyl)-furof2.3-blpvridine hydrochloride A 0.56 g sample of the compound from step 13b above was dissolved in 3 mL of CH2C12 and cooled to 0OC. The solution was stirred under N2, 3 mL of TFA was added, and the reaction mixture was stirred for 1 hour. The reaction mixture was poured into saturated K2C03, and the mixture was extracted with CH2C12. The solution was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 99:1 to 95:5 CHC13:MeOH. The product was converted to the salt by treatment with HCl/ether to give 0.36 g of title compound: 1H NMR (D20, 300 MHz) S 2.22-2.60 (m, 4H), 3.50-3.56 (m, 2H), 5.01 (t, 1H, J=8.1 Hz), 7.10 (s, 1H, ), 7.82 (d, 1H, J=2.3 Hz), 8.33 (d, 1H, J=2.3 Hz); MS m/z: 223 (M+H)+, 240 (M+NH4)+; Anal. Calcd for C 11 H 11 N20C1-1.0 HCI: C, 50.99; H, 4.67 N, 10.81. Found: C, 51.06; H, 4.64;
N, 10.65.
Example 14 Preparation of 5-chloro-2-(1-methyl-2-(S)-pyrrolidinXl)-furof 2.3-blpyridine dihvdrochloride A 200 mg (0.80 mmol) sample of 5-chloro-2-(2-(S)-pyrrolidinyl)-furo[2,3-b]pyridine dihydrochloride, from Example 13c above, was dissolved in 4 rnL of 88%
formic acid and 8 mL of 37% aqueous formaldehyde and heated at reflux for 1 hour. The reaction mixture was cooled, poured into saturated K2C03, and the mixture was extracted with CH2CI2. The extract was dried over MgSO4, and the solvent was removed.
The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 CHC13:MeOH. The product was converted to the salt by treatment with HCl/ether to give 140 mg of title SUBSTiTUTE SHEET (RULE 26) compound: 1H NMR (D20, 300 MHz) S 2.29-2.38 (m, 2H), 2.49-2.68 (m, 2H), 2.95 (br s, 3H), 3.44 (m, 1H), 3.84 (m, 1H), 4.84 (m, IH), 7.22 (s, 1H), 8.22 (d, 1H, J=2.3 Hz), 8.36 (d, 1H, J=2.4); MS m/z: 237 (M+H)+, 254 (M+NH4)+; Anal. Calcd for C12HON2OC1=1.0 HCl=0.3 H20: C, 51.74; H, 5.28; N, 9.73. Found: C, 51.74; H, 5.28;
5 N, 10.16.

Examnle 15 Preparation of 2-(Hexahvdro-lH-7a-Qvrrolizinyl)furof3.2-blpvridine hydrochloride 10 15a. 1.2,3.5.6.7-Hexahydrol2yrrolizinium perchlorate The title compound was prepared using the procedures of Miyano et al., Synthesis , 1978: 701-702, and J. Heterocyclic Chem., 19:1465-1468 (1982).
15b. 7a-EthvnYl-hexahvdro-1 H-pyrrolizine The compound from step 15a above (1.0 g, 4.8 mmol) was added to a solution of 15 0.5 M ethynylmagnesium bromide (29 mL, 14.3 mmol) in THF at room temperature. The reaction mixture was stirred for 45 minutes, quenched with 15% NaOH solution, and diluted with brine:water (1:1). The aqueous phase was extracted with CH202, and the organic phases were combined, dried (MgSO4), concentrated and chromatographed (silica gel; CHC13/MeOH, 90:10) to afford an amber oil (463 mg, 71%): 1H NMR (CDC13, 20 MHz) S 1.75-2.06 (m, 6H), 2.14-2.23 (m, 2H), 2.33 (s, 1H), 2.53-2.62 (m, 2H), 3.22-3.28 (m, 2H); MS (CI/NH3) m/z: 136 (M + H)+.
15c. 2-(Hexahvdro-1 H-7a-pvrrolizinvl)furo13.2-blpvridine 2-Iodo-3-pyridinol (902 mg, 4.1 mmol), copper(I) iodide (116 mg, 0.61 mmol), bis(triphenylphosphine)palladium(lI) chloride (119 mg, 0.17 mmol) and triethylamine (570 25 mL, 4.1 mmol) were combined in DMF (4.5 mL) and stirred for one hour. 7a-Ethynyl-hexahydro-lH-pyrrolizine(460 mg, 3.4 mmol) in DMF (1.2 mL) was added dropwise and the mixture was heated at 60 C for 18 h. The reaction mixture was allowed to cool to ambient temperature and 2 N aqueous HCl was added. The heterogeneous mixture was washed with Et20 (2X), basified with 15% NaOH solution and extracted with 30 (2X). The CH2C12 extracts were combined, dried (MgSO4) concentrated and chromatographed (silica gel; CHC13/MeOH, 96:4) to afford an amber oil which solidified upon storage at -20 C (405 mg, 52%): mp 39-41 C; IH NMR (CDC13, 300 MHz) S
1.86-1.97 (m, 6H), 2.24-2.34 (m, 2H), 2.68-2.76 (m, 2H), 3.21-3.28 (m, 2H), 6.77 (s, 1H), 7.12 (dd, J=8.5, 5 Hz, 1H), 7.65 (d, J=8.5 Hz, 1H), 8.46 (d, J=5 Hz, 1H); MS
(CI/NH3) 35 m/z: 229 (M + H)+.

SUBSTITUTE SHEET (RULE 26) 15d. 2-(Hexahvdro-lH-7a-vvrrolizinyl)furoi3.2-blp idine 1ydrochloride The free base (395 mg, 1.73 mmol), from step 15c above, was dissolved in THF
(30 mL) and a saturated solution of HCl in Et20 was added until precipitation ceased. The solvent was decanted and the remaining light yellow solid triturated with THF
(2X). The product was recrystallized from MeOH/Et20 to afford a light yellow powder, (349 mg, 76%): mp 201-203 C dec.; IH NMR (DMSO, 300 MHz) S 2.09-2.37 (m, 6H), 2.62-2.73 (m, 2H), 3.23-3.40 (m, 2H), 3.57-3.70 (m, 2H), 7.40 (dd, J=8, 5 Hz, 1H), 7.53 (s, 1H), 8.05 (dd, J=8, 1 Hz, 1H), 8.56 (dd, J=5, 1 Hz, 1H), 11.35 (br s); MS (CI/NH3) m/z: 229 (M +H)+; Anal. Calcd for C14H16N2O-1.1HC1: C, 62.81; H, 6.51; N, 10.42. Found:
C, 62.65; H, 6.42; N, 10.44.

Example 16 Preparation of 2-(Hexahydro-1 H-7a-pyrrolizinvl)-5-methylfurof3.2-blpyridine dihydrochloride 16a. 2-(Hexahydro-1 H-7a-pyrrolizinyl)-5-methylfuro13.2-blp 'dine The acetylene compound 7a-ethynyl-hexahydro-lH-pyrrolizine (450 mg, 3.33 nunol), 2-iodo-6-methyl-3-pyridinol (939 mg, 4.0 mmol)(Aldrich), copper(I) iodide (114 mg, 0.6 mmol), bis(triphenylphosphine)palladium(II) chloride (117 mg, 0.17 mmol) and triethylamine (560 mL, 4.0 mmol) were combined in a similar fashion as that described for Example id. The residue was chromatographed (silica gel; CHC13/MeOH, 96:4) to afford a yellow solid (403 mg, 50%): IH NMR (CDC13) S 1.85-1.95 (m, 6H), 2.25-2.30 (m, 2H), 2.62 (s, 3H), 2.67-2.75 (m, 2H), 3.19-3.26 (m, 2H), 6.68 (s, IH), 6.98 (d, J=8 Hz, IH), 7.54 (d, J=8 Hz, IH); MS (CI/NH3) m/z: 243 (M + H)+.
16b. 2-(Hexahvdro-lH-7a-pvrrolizinyl)-5-methvlfurof3,2-bjpyiidine dihydrochloride A sample of the compound from step 16a (395 mg, 1.63 mrnol) (395 mg, 1.63 mmol) was dissolved in CH202 (20 mL) and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed to afford a yellow oil/solid (390 mg, 72%): 1H
NMR (D20, 300 MHz) S 2.30-2.48 (m, 6H), 2.75-2.84 (m, 5H), 3.35-3.45 (m, 2H), 3.77-3.85 (m, 2H), 7.39 (s, 1H), 7.62 (d, J=9 Hz, 1H), 8.31 (d, J=9 Hz, 1H);
MS
(CI/NH3) m/z: 243 (M + H)+; Anal. Calcd for C15H 1 8N20-2.0 HC1-1.0 H20: C, 54.06;
H, 6.65; N, 8.41. Found: C, 54.00; H, 6.33; N, 8.11.

SUBSTITUTE SHEET (RULE 26) Exam le 17 Prenaration of 2-(Hexahvdro-lH-7a-pyrrolizinvl)furof2.3-clpvridine dihvdrochloride 17a. 2- (Hexahvdro-lH-7a-p,vrrolizinvl)furof2 3-cln, 'dine The acetylene compound 7a-ethynyl-hexahydro-lH-pyrrolizine (225 mg, 1.66 mmol), 4-iodo-3-pyridinol (441 mg, 2.0 mmol) from Example 9b, copper(I) iodide (60 mg, 0.30 mmol), bis(triphenylphosphine)-palladium(II) chloride (58 mg, 0.08 mmol) and triethylamine (280 mL, 2.0 mmol) were combined in a similar fashion as that described in Example 15c. The crude product was chromatographed (silica gel; CHC13/MeOH, 98:2 to 95:5) to afford a turbid yellow oil (185 mg, 49%): 1 H NMR (CDCl3, 300 MHz) S
1.83-1.97 (m, 6H), 2.24-2.31 (m, 2H), 2.67-2.75 (m, 2H), 3.19-3.26 (m, 2H), 6.62 (s, 1 H), 7.42 (d, J=5 Hz, 1H), 8.35 (d, J=5 Hz, 1H), 8.77 (s, 1H); MS (CI/NH3) m/z: 229 (M +
H)+.
17b. 2-(Hexahydro-lH-7a-pyrrolizinyl)furof2 3-clpyridine dihydrochloride A sample of the compound from step 17a (173 mg, 0.76 mmol) was dissolved in CH2C12 (10 mL) and a saturated solution of HCI in Et20 was added until precipitation ceased. The solvent was removed and the product was recrystallized from MeOH/Et20 to afford a light yellow solid (226 mg, 98%): mp 235-238 C; IH NMR (D20, 300 MHz) S
2.30-2.49 (m, 6H), 2.76-2.85 (m, 2H), 3.35-3.45 (m, 2H), 3.77-3.86 (m, 2H), 7.41 (s, iH), 8.04 (d, J=6 Hz, 1H), 8.49 (d, J=6 Hz, 1H), 9.05 (s, 1H); MS (CI/NH3) m/z: 229 (M + H)+; Anal. Calcd for C14H16N20=2.0 HCl=0.8 H20: C, 53.28; H, 6.26; N, 8.88.
Found: C, 53.61; H, 6.49; N, 8.35.

ExamQle 18 Prenaration of endo-2-(Hexahydro-lH-3-(R)-nvrrolizinyl)furof3 2-blpyridine dihydrochloride iy C
H
/ DN
18a 3-(N-BOC-2-(R)-12vrrolidinvl),plopenal To a solution of N-Boc-R-prolinal (10.25 g, 51.50 mmol) in 150 mL of anhydrous toluene at room temperature was added (triphenylphosphoranylidene)-acetaldehyde (17.2 g, 56.7 mmol), and the mixture was refluxed for 3 hours under nitrogen. The mixture was SUBSTITUTE SHEET (RULE 26) concentrated in vacuo. The residue was purified on silica gel, eluting with EtOAc/hexane. The title compound was obtained as an amber oil in 53% yield (6.13g): 1H
NMR (CDC13, 300 MHz) S 1.42 (s (major isomer), 9H), 1.49 (s (minor isomer), 9H), 1.73-1.90 (m, 3H), 2.06-2.24 (m, 1H), 3.37-3.54 (m, 1H), 4.41-4.52 (m (minor isomer), 1H), 4.58-4.68 (m (minor isomer), 1H), 6.11 (dd, 3.0 Hz, 8.0 Hz, 1H), 6.63-6.82 (m, 1H), 9.57 (s (minor isomer), 1H), 9.59 (s (major isomer), 1H); MS(DCI) (M+H)+:
226, (M+NH4)+: 243.
18 b. 3-(N-BOC-2-(R)-p~,rrolidinyl )propanal To a solution of the propenal compound from step 18a (27.20 mmol, 8.02 g) was added 100 mL of EtOAc and 0.5 g of 10% Pd/C. The mixture was agitated under 4 atmosphere of H2 for 16 hours. The catalyst was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was purified on silica gel, eluting with 1/4 EtOAc/hexane. The title compound was obtained in 97% yield as a yellow oil (5.99g): 1H
NMR (CDC13, 300 MHz) 5 1.48 (s, 9H), 1.60 (m, 1H), 1.69-2.01 (m, 5H), 2.39-2.52 (m, 2H), 3.21-3.36 (m, 2H), 3.73-3.94 (m, 1 H), 7.27 (s, 1H); MS (DCI) m/z:

(M+H)+, 245 (M+NH4)+.
18c. 5-(N-BOC-2-(R)-pygolidinyl)-3-hydroxy-I -pentyne A solution of the propanal compound from step 18b above (26.40 mmol, 5.99 g) in 100 mL of anhydrous THF under a nitrogen atmosphere was cooled to -78 C. To this solution was added ethynyl magnesium bromide (0.5 M in THF/79.20 mL), and the mixture was stirred at -78 C for one hour. The mixture was then warmed to room temperature and stirred for 1.5 hours. The reaction was quenched by pouring it into 200 mL of saturated NH4C1. The mixture was extracted with CH202, and the extracts were dried over Na2SO4 and concentrated under vacuum. The residue was purified on silica gel, eluting with EtOAc/hexane (1/3). The title compound was obtained in 90% yield as a light yellow oil (5.99 g): I H NMR (CDC13, 300 MHz) S 1.47 (s, 9H), 1.58-2.00 (m, 8H), 2.41-2.49 (br s, 1H), 3.24-3.37 (m, 3H), 3.66-4.01 (br d, 1H), 4.31-4.51 (m, 1 H); MS (DCI) m/z: 254 (M+H)+; 271 (M+NH.q)+.
18d. endo-hexahvdro-lH-3-(R)-ethvnvlpyrrolizine and exo-hexahydro-lH-3-(S)-ethynylpyrrolizine To a solution of the alcohol compound from step 18c above (17.30 mmol, 4.38 g) in CH2C12 (30 mL) at room temperature was added triphenylphosphine dibromide (21.60 mmol, 9.12g), and the mixture was stirred for 16 hours. Next, 5 mL of TFA was added followed by stirring for another 4 hours at room temperature. The mixture was then concentrated under vacuum. The residue was purified by chromatography on silica gel, eluting with 10% MeOH/CH2C12 containing 1% NH4OH, to separate the exo and endo products The combined yield for the reaction was 64%.

SUBSTITUTE SHEET (RULE 26) endo-(R)- compound: [a]23D -42.76 (c 0.14, H20); IH NMR (CDC13, 300 MHz) 8 1.32-1.48 (m, 2H), 1.69-1.74 (m, 4H), 1.75-2.04 (m, 2H), 2.38 (m, 1H), 2.75 (d, J=2.0 Hz, 1H), 2.67 (m, 1H), 3.07 (m, IH), 3.36 (m, 1H), 3.60 (m, 1H); MS
(DCI) m/z:
136 (M+H)+, 153 (M+NH4)+; Anal. Calcd for C9H13N=0.20 H20: C,77.87; H, 9.73;
N, 10.09. Found: C, 78.15; H, 9.87; N, 10.17.
exo-(S)- compound: [a]23D +51.14 (c 0.37, H20); IH NMR (CDC13, 300 MHz) 8 1.42 (m, 1 H), 1.62 (m, 1 H), 1.88 (m, 1 H), 1.89-2.19 (m, 5H), 2.22 (d, J=2.0 Hz, 1 H), 2.90 (m, 1H), 3.07 (m, 1H), 3.53 (m, 1H), 3.88 (m, 1H); MS (DCI) m/z: 136 (M+H)+, 153 (M+NH4)+; Anal. Calcd for C9H13N=0.10 H20: C,78.89; H, 9.71; N, 10.22.
Found: C, 78.88; H, 9.59; N, 10.05.
18e. endo-2-(hexahvdro-lH-3-(R)-Rvrrolizinyl)furo[3 2-blRvridine dihydrochloride To DMF (5.0 mL) in a flask purged with nitrogen was added 2-iodo-3-hydroxypyridine (1.20 mmol, 0.2652 g)(Aldrich), bis(triphenylphosphine)-palladium(II) chloride (0.05 mmol, 35 mg), copper(I)iodide (0.20 mmol, 38.1 mg), and triethylamine (1.2 mmol, 0.1214 g), and the mixture was stirred for one hour at room temperature. The endo-(R)-acetylene compound from step 18d above (1.0 mmol, 0.134 g in 5.0 mL
of DMF) was then added and the mixture was heated at 60 0 C for 16 hours. The mixture was cooled and poured into 2 N HC1(100 mL), and the mixture was extracted with CH2C12 (2x75 mL).
The aqueous layer was basified with solid K2C03 and extracted with CH2C12, and the extract was dried over MgSO4 and concentrated under vacuum. The residue was purified on silica gel, eluting with 10% MeOH in CH2C12. The title compound was obtained by treating the base with a saturated solution of HCl/EtOH at 0'C: [a]D23 +26.21 (c 0.12, MeOH); IH NMR (CDC13, 300 MHz) 8 1.96-2.13 (m, 2H), 2.15-2.40 (m, 3H), 2.51-2.78 (m, 3H), 3.57-3.69 (m, 2H), 4.45-4.57 (m, 1 H), 4.96-5.08 (m, 1 H), 7.65 (bs, 1 H), 7.95-8.07 (m, IH), 8.28-8.37 (m, 1H), 8.51-8.71 (d, J=10,0 Hz, 1H); MS (DCI) m/z:
229 (M+H)+, 246 (M+NH4)+; Anal. Calcd for C14H16N20=2.2 HCl=1.1 H20: C, 51.21;
H, 6.26; N, 8.53. Found: C, 51.27; H, 6.05; N, 8.31.

SUBSTITUTE SHEET (RULE 26) Example 19 Preparation of exo-2-(Hexahvdro-1 H-3-(S)-pyrrolizinyl)furof 3.2-blpvridine dihydrochloride O
5 Following the procedures of Example 18e above, substituting the exo-hexahydro-1H-3-(S)-ethynylpyrrolizine compound from Example 18d above for the endo-(R) compound of step 18e, the title compound was prepared. [a]D23 -21.28 (c 0.10, MeOH);
1 H NMR (CDC13, 300 MHz) S 1.78-2.00 (m, 2H), 2.06-2.24 (m, 2H), 2.36-2.52 (m, 2H), 2.53-2.78 (m, 2H), 2.90-3.03 (m, 1H), 3.21-3.30 (m, 1H), 4.42-4.53 (m, 1H), io 5.17-5.29 (m, 1H), 7.61 (s, 1H), 7.89 (dd, J=9.0 Hz, J=11.0 Hz, 1H), 8.62 (d, J=12.0 Hz, 1H), 8.27-8.38 (m, 1H); MS (DCI) m/z: 229 (M+H)+, 246 (M+NH4)+; Anal.
Calcd for C14H16N2O=2.40 HCl=0.50 H20: C, 51.87; H, 6.02; N, 8.49. Found: C, 51.87;
H, 5.71; N, 8.49.

15 ExampJe 20 Preparation of exo-2-(Hexahydro-1 H-3-(R)õuyrrolizinY)furof 3.2-b]pvridine dihydrochloride H

C
-~
H
O / N
, ~

Following the procedures of Example 18, substituting (S)-prolinal for the (R)-20 starting material of step 18a therein, and carrying the reactions forward as in steps 18b, c, and d, then separating the exo-(R)- and endo-(S)-isomers and carrying the exo-(R)-compound forward according to the procedures of step 18e, the title compound was -prepared. The MS and NMR data were similar to the compound of Example 18e.
[aID23 24.68 (c 0.16, MeOH).

SUBSTiTUTE SHEET (RULE 26) F.xa le 21 Preparation of endo-2-(Hexahvdro-lH-3-(S)-p,yrrolizinvl)furof3.2-blp i~r dine dihydrochloride H

N
~

Following the procedures of Example 18e above, substituting the endo-hexahydro-1H-3-(S)-ethynylpyrrolizine compound from Example 20 above for the endo-(R) compound of step 18e, the title compound was prepared. The MS and NMR data were similar to the compound of Example 18e. [a]D23 +31.01 (c 0.21, MeOH).

Example 22 Preparation of 1-Pyrrolidinylmethyl-(2-furo[3.2-blpyridine) To DMF (20.0 mL) in a flask purged with nitrogen were added 2-iodo-3-hydroxypyridine (18.60 mmol, 4.11 g)(Aldrich), bis(triphenylphosphine)-palladium(II) t s chloride (0.80 mmol, 0.544 g), copper(I) iodide (3.10 mmol, 0.590 g), and triethylamine (18.60 mmol, 2.59 g), and the mixture was stirred for one hour at room temperature. To this solution was then added N-(3-propynylpyrrolidine (15.50 mmol, 1.68 g, prepared according to Biehl and DiPierro, J. Am. Chem. Soc., 80, 4609-4614, 1958), in DMF
(10.0 mL), and the mixture was heated at 60 OC for 16 hours. The mixture was cooled, poured into 4 N HCl (100 mL) and extracted with CH2C12. The aqueous phase was then basified with 15% NaOH and extracted with CH202. The extract was dried over MgSO4 and concentrated in vacuo. The residue was purified by chromatography, eluting with 10%
MeOH/CH2C12 to give the title compound in 72% yield: 1 H NMR (300 MHz): S 2.25 (bs, 4H), 3.64 (bs, 4H), 4.88 (s, 2H), 7.57 (s, 1 H), 7.90 (dd, J=5.37, 13.67 Hz, IH), 8.56 (d, J=8.30 Hz, 1H), 8.80 (d, J=5.86 Hz, 1H). MS (DCI): (M+H)+, 203; (M+NH4)+
220. Anal. Calcd for C12H14N20=2.0 HCI=0.1 H20: C, 52.03; H, 5.89; N,10.11.
Found C, 51.72; H, 6.12; N, 10.05 SUBSTiTUTE SHEET (RULE 26) F.xa nie 23 Prevaration of 5-Chloro-2-(hexahvdro-lH-7a-p olizinvl)furof3.2-blp, idine hydrochloride 23a. 5-Chloro-2-(hexahvdro- IH-7a-pyrrolizinyl)furof3.2-binvridine 7a-Ethynyl-hexahydro-1 H-pyrrolizine from Example 15b (225 mg, 1.66 mol, 6-chloro-2-iodo-3-pyridinol (509 mg, 2.0 mmol), copper(I) iodide (60 mg, 0.30 mmol), bis(triphenylphosphine)palladium(II) chloride (58 mg, 0.08 mmol) and triethylamine (0.280 mL, 2.0 mmol) were combined in a similar fashion as that described in Example 15c. The crude product was chromatographed (silica gel; CHC13/MeOH, 97.5:2.5) to afford a waxy tan solid (335 mg, 77%): 1H NMR (CDC13, 300 MHz) S 1.88-1.97 (m, 6H), 2.21-2.33 (m, 2H), 2.67-2.79 (m, 2H), 3.19-3.26 (m, 2H), 6.70 (s, 1H), 7.14 (d, J=8.5 Hz, IH), 7.61 (d, J=8.5 Hz, 1H); MS (CI/NH3) m/z: 263 (M + H)+.

23b. 5-Chloro-2-(hexahydro-1 H-7a-pyrrolizinvl)furof 3.2-blpvridine hydrochloride The compound from step 23a (325 mg, 1.24 mmol) was dissolved in CH2C12 (10 mL) and a saturated solution of HC1 in Et20 was added dropwise. The solvent was removed and the product recrystallized from MeOH/Et2O to afford a white solid (223 mg, 58%): mp 233-235 C; IH NMR (D20, 300 MHz) S 2.32-2.45 (m, 6H), 2.75-2.83 (m, 2H), 3.31-3.43 (m, 2H), 3.75-3.83 (m, 2H), 7.23 (s, 1H), 7.49 (d, J=9 Hz, 1H), 8.01 (d, J=9 Hz, 1H); MS (CI/NH3) m/z: 263 (M + H)+; Anal. Calcd for C14H15C1N2O=1.2 HCI: C, 54.86; H, 5.33; N, 9.14. Found: C, 54.61; H, 5.36; N, 8.98.

Examnle 24 Preparation of 2-(Hexahydro-lH-7a-Ryilolizinyl)thienof3.2-blRvridine hydrochloride 24a. 2-(Hexahydro-1 H-7a-pvrrolizinvl)thieno[3.2-blpyridine Thieno[3,2-b]pyridine (200 mg, 1.48 mmol) prepared according to S. Gronowitz et al., Acta Chemica Scandinavica B 1975, 29: 233-238 was dissolved in THF (6 mL,) and nBuLi in hexanes (2.5 M, 0.6 mL, 1.5 mmol) was added at 0 C. After 10 minutes of stirring, 1,2,3,5,6,7-hexahydropyrrolizinium perchlorate from Example 15a (155 mg, 0.74 mmol) was added. The slurry was allowed to gradually warm to ambient temperature and stir for an additional two hours. The reaction mixture was partitioned between 2 N aqueous HCl and Et20. The phases were separated and the aqueous phase was basified with 15%
NaOH solution and then extracted with CH202 (3X). The CH2CI2 extracts were combined, dried (MgSO4) and concentrated. The residue was chromatographed (silica gel;
CHC13/MeOH, 100:0 to 99:1) to afford a yellow solid (115 mg, 63%): mp 94-96 C; 1 H
SUBSTITUTE SHEET (RULE 26) NMR (CDC13, 300 MHz) S 1.80-1.96 (m, 4H), 2.00-2.09 (m, 2H), 2.18-2.26 (m, 2H), 2.64-2.72 (m, 2H), 3.23-3.30 (m, 2H), 7.13 (dd, J=8, 4 Hz, 1H), 7.23 (s, 1H), 8.05 (dd, J=8, 1.5 Hz, 1H), 8.58 (dd, J=4, 1.5 Hz, IH); MS (CI/NH3) m/z: 245 (M + H)+.
24b. 2-(Hexahvdro-lH-7a-12 olizinyl)thieno13.2-blp,yridine hydrochloride The compound from step 24a (104 mg, 0.43 mmol) was dissolved in CH202 (3 mL) and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed and the product was dried in vacuo to afford a hygroscopic yellow solid (124 mg, 93%):
1 H NMR (D20, 300 MHz) S 2.30-2.48 (m, 4H), 2.51-2.60 (m, 2H), 2.77-2.86 (m, 2H), 3.36-3.44 (m, 2H), 3.79-3.88 (m, 2H), 7.67 (dd, J=8, 5 Hz, 1H), 7.84 (s, 1H), 8.70 (dd, J=8, 1 Hz, 1H), 8.75 (dd, J=5, 1 Hz, IH); MS (CI/NH3) m/z: 245 (M + H)+; Anal.
Calcd for C 14H 16N2S= 1.8 HCI: C, 54.25; H, 5.79; N, 9.04. Found: C, 54.25; H, 5.81; N, 8.75.

Examlale 25 Preparation of 5.6-Dichloro-2-(2-(S)-12vrrolidinvl)furof3.2-blpyridine hydrochloride 5.6-dichloro-2-(1-t-butyloxvcarbonvl-2-(S)-12vrrolidinyl)furof 3.2-blp3jidine 5,6-Dichloro-2-iodo-3-pyridinol (750 mg, 2.6 mmol)(prepared by treatment of 5,6-dichloro-3-hydroxypyridine (Koch & Schnatterer, Synthesis 1990, 499) with 12 (ibid, p.
497), copper(l) iodide (89 mg, 0.47 mmol), bis(triphenylphosphine)palladium(II) chloride (91 mg, 0.13 mmol) and triethylamine (433 mL, 3.1 mmol) were combined in DMF
(3.0 mL) and allowed to stir for 1 hour. 1-t-Butyloxycarbonyl-2-(S)-ethynylpyrrolidine (610 mg, 3.1 mmol) in DMF (1 mL) was added and the reaction mixture was heated to 60 C
for 16 hours. After cooling to ambient temperature, the reaction mixture was poured over Et-20/saturated K2C03 solution and the phases separated. The organic phase was washed with brine:water (1:1) (4X), dried (MgSO4), concentrated and chromatographed (silica gel;
EtOAc/hexane, 1:6) to afford an amber oil (408 mg, 44%): 1 H NMR (CDC13, 300 MHz) S
1.32 and 1.45 (two br s, 9H), 1.95-2.40 (m, 4H), 3.45-3.74 (m, 2H), 5.02 (m, 1H), 6.62 (s, 1H), 7.81 (s, 1H); MS (CI/NH3) m/z: 357 (M + H)+.
25b. 5.6-Dichloro-2-(2-(S)-pyrrolidinyl)furof3.2-blp, iry dine The compound from step 25a (400 mg, 1.12 mmol) was dissolved in CH2C12 (3 mL) and TFA (3 mL) was added at ambient temperature. After stirring for 1 hour, the solvent was removed and the residue was dissolved in CH202 and washed with saturated K2C03 solution, dried (MgSO4) and concentrated. The crude product was chromatographed (silica gel; CHC13/MeOH, 98:2) to afford a solid (206 mg, 71 %): mp 98-SUBSTITUTE SHEET (RULE 26) 100 C; 1H NMR (CDC13, 300 MHz) 6 1.81-2.05 (m, 3H), 2.22 (m, 1H), 3.04-3.20 (m, 2H), 4.40 (m, 1H), 6.70 (s, 1H), 7.80 (s, 1H); MS (CI/NH3) m/z: 257 (M + H)+.
25c. 5.6-Dichloro-2-(2-(S)-pyrrolidinvl)furoi3.2-bl123ridine hydrochloride The compound from step 25b above (54 mg, 0.21 mmol) was slurried in Et20 and a saturated solution of HCI in Et20 was added dropwise. The solvent was removed and the product was recrystallized from MeOH/Et2O to afford a white solid (48 mg, 78%): [aJD2o +5.3 (c 0.51, MeOH); 1H NMR (D20, 300 MHz) S 2.18-2.65 (m, 4H), 3.51-3.56 (m, 2H), 5.05 (dd, J=8, 8 Hz, 1H), 7.16 (d, J=1 Hz, 1H), 8.24 (d, J=1 Hz, 1H); MS
(CI/NH3) m/z: 257 (M + H)+; Anal. Calcd for C 11 H l OC12N2O=HCI: C, 45.00; H, 3.78;
N, 9.54. Found: C, 45.08; H, 3.59; N, 9.40.

Example 26 Preparation of 5 .6-Dichloro-2-(1-methk-2-(S )-pyrrolidinvl)furof 3,2-blpyridine hydrochloride 26a. 5.6-Dichloro-2-(1-methyl-2-(S )-p=olidinvl)furo[3.2-blpyridine The amine from Example 25b (145 mg, 0.57 mmol) was dissolved in an aqueous solution of 37% formaldehyde (excess) and 88% formic acid (excess). The aqueous mixture was heated to 90 C for 1.5 hours and then allowed to cool to ambient temperature. The reaction mixture was washed with Et20, basified with 15% NaOH solution and extracted with CH2C12 (3X). The organic phases were combined, dried (MgSO4), concentrated and chromatographed (silica gel; CHC13/MeOH, 98:2) to afford a white solid (97 mg, 62%): mp 58-60 C; 1H NMR (CDC13, 300 MHz) S 1.89 (m, 1H), 2.00-2.17 (m, 2H), 2.24 (m, 1H), 2.33 (s, 3H), 2.39 (m, 1H), 3.26 (m, 1H), 3.43 (m, 1H), 6.74 (s, 1H), 7.83 (s, 1H);
MS (CI/NH3) m/z: 271 (M + H)+.
26b. 5,6-Dichloro-2-(1-methyl-2-(S)-pyrrolidinyl)furof3,2-blp3 'dirine hydrochloride The compound from step 26a (92 mg, 0.34 mmol) was slurried in Et20 and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed and the product was recrystallized from MeOH/Et20 to afford a white solid (70 mg, 67%): mp 249-251 C; 1H NMR (D20, 300 MHz) S 2.27-2.37 (m, 2H), 2.47-2.71 (m, 2H), 2.93 (s, 3H), 3.38 (m, 1H), 3.79 (m, 1H), 4.82 (m, partially buried under H20 peak, 1H), 7.27 (s, 1H), 8.26 (s,.1H); MS (CI/NH3) m/z: 271 (M + H)+; Anal. Calcd for C12H12C12N20=1.2 HCI: C, 45.77; H, 4.23; N, 8.82; Found: C, 45.61; H, 4.36; N, 8.90.

SUBSTITUTE SHEET (RULE 26) Examnl Pr!Maration of 2-(Hexahvdro-lH-7a_pvrrolizinyl)-4-methylthieno13.2-blpvridine dihydrochloride 5 27a. 2-(Hexahvdro-1 H-7a-2Yrrolizinvl)-4-methvlthienof 3.2-binvridine 5-Methylthieno[3,2-b]pyridine (285 mg, 1.91 mmol, prepared according to Gronowitz et al., Acta Chemica Scandinavica B, 29:233-238 (1975)), diisopropylamine (270 uL, 1.91 mmol) and nBuLi (2.5 M in hexanes, 765 uL, 1.91 mmol) were combined in THF (8 mL).
After 20 minutes of stirring, 1,2,3,5,6,7-hexahydro-pyrrolizinium perchlorate from 10 Example 15a (200 mg, 0.95 mmol) was added. The nmixture was allowed to warm to ambient temperature and 2 N aqueous HC1 was added. The reaction mixture was washed with Et20 and the aqueous phase was basified with 15% NaOH solution and extracted with CH2C12 (3X). The organic phases were combined, dried (MgSO4), concentrated and chromatographed (silica gel; CHC13/MeOH, 99:1) to afford a solid (79 mg, 32%):

15 NMR (CDC13, 300 MHz) S 1.80-1.94 (m, 4H), 1.98-2.07 (m, 2H), 2.16-2.24 (m, 2H), 2.64 (s, 3H), 2.64-2.71 (m, 2H), 3.22-3.28 (m, 2H), 7.02 (d, J=8 Hz, 1H), 7.16 (s, 1H), 7.92 (d, J=8 Hz, 1H); MS (CI/NH3) m/z: 259 (M + H)+.
27b. 2-(Hexahydro-lH-7a-pyirolizinvl)-4-methylthienof3.2-blpyridine dihydrochloride The compound from step 27a (73 mg, 0.28 mmol) was dissolved in CH2C12 and 20 treated with a saturated solution of HCl in Et20 to afford a hygroscopic foam-like solid (100 mg, quantitative): mp 233-235 C; 1H NMR (D20, 300 MHz) 8 2.28-2.49 (m, 4H), 2.55-2.64 (m, 2H), 2.77-2.86 (m, 2H), 2.89 (s, 3H), 3.38-3.47 (m, 2H), 3.82-3.90 (m, 2H), 7.78 (d, J=8.5 Hz, 1H), 7.90 (s, 1H), 8.90 (d, J=8.5 Hz, 1H); MS (CI/NH3) m/z:
259 (M
+ H)+; Anal. Calcd for C 15H 1 gN2S= 1.3 HCl=0.9 H20: C, 50.26; H, 6.21; N, 7.81.
25 Found: C, 50.68; H, 6.40; N, 7.41.

Examule 28 Prgparation of 5-bromo-2-(2-(S)-p,vrrolidinyl)-furof3.2-bipvridine hydrochloride 30 28a. 5-amino-2-bromopyridine A mixture of 2-bromo-5-nitropyridine (30.75 g, 151.5 mmol), water (250 mL), and acetic acid (110 mL) was heated to 45 C. Iron powder (24.5 g, 439 mmol) was added at a rate which kept the temperature below 53 C, then the mixture was stirred at 48 C 5 C.
The nzixture was cooled to room temperature and filtered through diatomaceous earth. The 35 filter cake was -washed with EtOAc, and the aqueous mixture was extracted with EtOAc.
The combined organic fractions were washed with saturated Na2CO3 and brine, dried over MgSO4, and the solvent was removed in vacuo. The residue was chromatographed on silica SUBSTITUTE SHEET (RULE 26) gel, eluting with 100:0 to 50:50 hexane:EtOAc to give 20.4 g of the title compound: 1 H
NMR (CDC13 300 MHz) 8 6.86-6.90 (dd, 1H, J=8.5, 2.4 Hz) 7.21-7.23 (d, 1H, J=8.2 Hz) 7.85-7.86 (d, 1H, J=3 Hz); MS m/z: 173 (M+H)+, 190 (M+NH4)+.
28b. 5-acetoxy-2-bromoA y'~dine To 25.6 mL of boron trifluoride etherate (208 mmol, Aldrich) cooled to -15 C
under N2 was added 18 g (104 mmol) of 5-amino-2-bromopyridine (from step 28a above) dissolved in 35 mL of DME. Then tert-butyl nitrite (14.7 mL, 125 mmol, Aldrich) was added at a rate which kept the temperature below 0 C. DME (65 mL) and CH2C12 (60 mL) were then added. After 10 minutes at -10 C the mixture was warmed to 5 C
and stirred for 30 min. Pentane (400 mL) was then added to the reaction mixture, the solid was collected by suction filtration, washed with cold ether, air dried, and dissolved in 125 mL
acetic anhydride. The resulting solution was heated to 100 C 5 C for 1 hour. The solvent was removed in vacuo, and the residue was suspended in saturated aqueous Na2CO3, and extracted with ethyl ether. The ether solution was dried over MgSO4, the solvent was removed in vacuo, and the residue was chromatographed on silica gel, eluting with 100:0 to 60:40 hexane:EtOAc to give 13.6 g of the title compound: I H NMR (CDC13 300 MHz) S
2.35 (s, 3H) 7.36-7.39 (dd, 1H), 7.49-7.52 (d, IH), 8.19-8.21 (d, 1H) MS m/z:

(M+H)+, 233 (M+NH4)+.
28c. 2-bromo-5-hydroxypvridine 5-Acetoxy-2-bromopyridine (12.8 g, 60 mmol, from step 28b) was dissolved in 15% aqueous NaOH (50 mL) at 0 C, and the solution was warmed to room temperature and stirred for 60 minutes. After complete consumption of the starting material the solution was neutralized by addition of 1 N HCI. The aqueous mixture was extracted with EtOAc (3 X
200 ml.). The organic extracts were washed with brine (4 X 50 mL), water (2 X
50 mL), dried (MgSO4), and the solvent was evaporated to yield 9.8 g of the title compound: IH
NMR (CDC13, 300 MHz) 8 7.12-7.16 (dd, 1H, J=3.2 Hz),7.36-7.39 (d, 1H, J=8.5Hz), 8.04-8.05 (d, 1H, J=2.4 Hz); MS m/z: 174 (M+H)+.
28d. 6-bromo-2-iodo-3-12, 'dinol A 4.125 g sample of 2-bromo-5-hydroxypyridine (from step 28c) and 5.28 g of Na2CO3 were dissolved in 75 mL of water. To this solution was added 6.02 g of 12, and the mixture was stirred until the iodine color disappeared. The reaction mixture was then adjusted to pH 5, and extracted with EtOAc. The extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 97:3 CHC13:MeOH to give 4.3 g of the title compound: I H NMR (CDC13, 300 MHz) S
7.08-7.11 (d, 1H, J=8.5 Hz), 7.29-7.32 (d, 1H, J=8.5 Hz); MS m/z: 300 (M+H)+, 317 (M+NH4)+.

SUBSTiTUTE SHEET (RULE 26) 28e 2-(1-BOC-2-(S)-pyrrolidinvl)-5-bromofurol3.2-blpyridine A 1.84 g(6.10 mmol) sample of 6-bromo-2-iodo-3-pyridinol, from step 28d above, was dissolved in 10 mL of DMF, and (Ph3P)2PdCI2 (0.30 g, 0.4 mmol), CuI (0.3 g, 1.6 mmol) and triethylamine (1.2 mL, 8.5 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 1.3 g (6.7 mmol) of 1-BOC-2-(S)-ethynylpyrrolidine, from Example lc above, dissolved in 5 mL of DMF, was added carefully. The reaction was stirred at 80 OC for 16 hours, then cooled to room temperature.
The reaction mixture was diluted with ether, then washed with 50% brine, and the extract was dried over MgSO4. The solvent was removed, and the residue was chromatographed on silica gel, eluting with 100:0 to 60:40 hexane:EtOAc to give 1.4 g of title compound: IH
NMR (CDC13, 300 MHz) S 1.31(s, 9H), 1.89-2.06 (m, 3H), 2.27-2.34 (m, 1H), 3.43-3.5 (m, 2H), 4.96-5.0 (m, 1H), 6.72 (s, 1H), 7.38-7.41 (d, 1H, J =8.6 Hz), 7.83-7.86 (d, 1H, J =8.6 Hz); MS m/z: 367 (M+H)+.
28f. 2-(2-(S)-12vrrolidinvi)-5-bromofurof3.2-biny_ridine hydrochloride is To a solution of the product from step 28e above (1.2 g) in 10 mL of CH2C12 at 0 0 C was added 10 mL of TFA. The reaction mixture was stirred for 1 hour, the mixture was poured into saturated K2C03, and the aqueous solution was extracted with CH202. The organic extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 99:1 to 95:5 CHCI3:MeOH. The residue was converted to the salt by treatment with HCI/ether to give 0.6 g of the title compound: IH
NMR (D20, 300 MHz) S 2.30-2.63 (m, 4H), 3.51-3.56 (m, 2H), 5.02-5.07 (t, 1 H, J=7.7 Hz), 7.15 (s, 1H,), 7.61-7.64 (d, 1H, J=8.8 Hz), 7.91-7.95 (d, 1H, J=8.8 Hz);
MS m/z:
267 (M+H)+, 282 (M+NH4)+; Anal. Calcd for C11H11N2OCl=1.0 HCI: C, 43.52; H, 3.98 N, 9.23. Found: C, 43.53; H, 4.08; N, 9.13.
282 2-(1-methvl-2-(S)-pyrrolidinyl)-5-Br-furof3.2-blQyddine dihydrochloride A 300 mg sample of the compound from step 28f above was dissolved in an aqueous solution of 37 % formaldehyde (4 mL) and 88 % formic acid (2 mL) and heated at reflux for 1 hour. The solution was cooled, diluted with water, and adjusted to pH 10 with K2C03. The mixture was extracted with CH202, and the extract was dried and concentrated. The residue was purified by chromatography on silica gel, eluting with 100:0 to 97:3 CHC13:MeOH. The product was dissolved in ethanol at ambient temperature and a solution of hydrochloric acid in Et20 was added dropwise. The resultant white precipitate was then collected by evaporation of solvent and triturated with three portions of Et20 to give the title compound (163 mg, 43 %): 1 H NMR (D20, 300 MHz) S 2.28-2.39 (m 2H), 2.49-2.72 (m, 2H), 2.95 (s, 3H), 3.38 (m, IH), 3.80 (m, 1H), 4.85 (m, 1H), 7.27 (s, 1H), 7.96 (d, 1H, J=1.02 Hz), 7.96 (d, 1H, J=1.02 Hz); MS m/z: 281 (M+H)+;
Anal.
SUBSTITUTE SHEET (RULE 26) Calcd for C12H 13N2OBr= 1.0 HCI: C, 45.38; H, 4.44 N, 8.82. Found: C, 45.11;
H, 4.17;
N, 8.52.

Exam l2e 29 Preparation of 5-methvl-2-(2-(R)-pyrrolidinyl)-furof3,2-blpvridine hydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 5 above: [a]D23 +16.5 (c 1.0, MeOH); Anal. Calcd for C12H14N20=2.0 HCl=0.2 H20=0.2 ethanol: C, 51.39; H, 6.19; N, 9.67. Found: C, 51.63;
H, 6.49; N, 9.33.

Exam l~ 30 Preparation of 5-methyl-2-(1-methvl-2-(R)-pvrrolidinyl)-furof 3,2-blpvridine dihydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 6 above: Anal. Calcd for C13H1 f,N20=2.0 HCl=0.4 H20: C, 52.68; H, 6.39; N, 9.145. Found: C, 52.70; H, 6.27; N, 9.32.

Examole 31 Preparation of 6-chloro-2-(2-(R)-pvrrolidinyl)-furof3.2-blpvridine hydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 13 above: Anal. Calcd for C 11H 11 N20C1= 1.0 HCI:
C, 50.99;
H, 4.67 N, 10.81. Found: C, 50.91; H, 4.75; N, 10.86.

Example 32 Preparation of 5-chloro-2-(1-methyl-2-(R)-pyrrolidinXl)-furo[3.2-b]pvridine hydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 12 above: Anal. Calcd for C12H13N20C1=1.8 HCI: C, 47.67;
H, 4.93; N, 9.27. Found: C, 47.49; H, 5.08; N, 8.97.

SUBSTITUTE SHEET (RULE 26) Examnle 33 Preparation of 5-bromo-2-(2-(R)-p3rrolidinvl)-furof3.2-b]pvridine hydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Examples 28 above: Anal. Calcd for Ci1HitN2OC1=1.0 HCI: C, 43.52;
H, 3.98 N, 9.23. Found: C, 43.40; H, 4.05; N, 8.98 ExamQle 34 Preparation of 2-(2-(R)-pyrrolidinyl)furof 2.3-c]pvridine dihydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 9 above: Anal. Calcd for C11H12N20=2 HCI: C, 50.58;
H, 5.40; N, 10.73. Found: C, 50.38; H, 5.37; N, 10.51.

Examnle 35 Prenaration of 5-chloro-2-(2-(R)-pyrrolidinvl)-furof3 2-bipvridine hydrochloride The title compound was prepared from 1-Boc-2(R)-ethynylpyrrolidine according to the procedures of Example 11 above: Anal. Calcd for C 11 H 11 N20C1=2 HCI: C, 50.99; H, 2o 4.67; N, 10.81. Found: C, 50.90; H, 4.75; N, 10.86.

Examllle 36 Preparation of 2-(2-(S)-pvrrolidinvl)furof2 3-blpvridine hydrochloride 3ba 2-(1-BOC-2-(S)-pyrrolidinyl)furof2 3-blpyridine The compound from step 13b above (0.23 g, 0.7 mmol), triethylamine (0.2 mL, 1.4 mmol), and 10% Pd on C (Aldrich, 50 mg) were stirred in 20 mL of EtOH under H2 (1 atm) for 4 hours. The mixture was filtered, concentrated and the crude product was purified by flash chromatography on silica gel eluting with hexane/EtOAc (9:1 to 7:3) to provide 140 mg (68%) of the title compound: 1H NMR (DMSO, 120 C, 300 MHz) S 1.33 (s, 9H), 1.93-2.10 (m, 3H), 2.32 (m, 1H), 3.46-3.53 (m, 2H), 5.0 (m, 1H), 7.28 (dd, 1H, J= 6.7, 2.8 Hz), 8.0 (dd, 1H, J=6.0, 1.7 Hz), 8.22 (dd, J=4.0, 1.4 1H); MS m/z: 289 (M+H)+, 306 (M+NH4)+
36b. 2-(2-(S)-pyrrolidinvl)furof2 3-blpvridine hydrochloride The compound from step 36a above (0.13 g, 0.45 mmol) was dissolved in 3 mL of CH2C12 at 0 C and 3 mL of TFA was added. The reaction mixture was stirred for I hour, poured into saturated aqueous K2C03, and extracted with CH2C12. The organic extract SUBSTiTUTE SHEET (RULE 26) was dried over Mg04, and the solvent was removed. The residue was chromatographed on silica gel, eluting with 99:1 to 95:5 CHC13:MeOH. The residue was treated with a solution of HCI in Et20 to give 40 mg (42 %) of title compound: IH NMR (D20, 300 MHz) S
2.15-2.62 (m, 4H), 3.48-3.75 (m, 2H), 5.01 (t, 1H, J=7.8 Hz), 7.11 (s, 1H, ), 7.43 (m, 5 1 H, J, 8.18 (dd, 1 H, J=7.8, 1.7 Hz) 8.33 (dd, 1H, J=7.8, 4.1, 2.4 Hz). MS
m/z: 189 (M+H)+, 206 (M+NH4)+; Anal. Calcd for C I 1 H 12N20= 1.4 HCI: C, 55.22; H, 5.64 N, 11.79. Found: C, 55.11; H, 5.41 N, 11.59 Examnle 37 10 Preparation of 2-(1-meth 1-~ 2-(S)-pyrrolidinvl)furof 3.2-cipvridine dihvdrochloride 37a. 4-hydroxy-3-iodop, idine To a solution of 4-hydroxypyridine (4.76 g, 50.1 mmol) and Na2CO3 (10.8 g, 100 mmol) in 200 mL of water was added 12 (12.7 g, 50.1 mmol). The reaction mixture was 15 stirred for 14 h then adjusted to pH 5 with concentrated HCI. The resulting solids were suspended in boiling ethanol and hot filtered. The solvent was removed and the resulting solids recrystallized from MeOH to afford 5.1 g (46%) of the title compound:

(DMSO-d6) d 8.26 (br s, 1H), 7.70 (s, 1H), 7.69 (d, J = 7 Hz, 1H), 6.14 (d, J
= 7 Hz, 1H); MS (DCI/NH3) m/z: 222 (M+H)+, 239 (M+NH4)+.

37b. 2- (1-BOC-2-(S )-pvrrolidinyl)furo f 3.2-clpyridine A sample of the compound from step 1 c above (1.95 g 12 mmol) was dissolved in 15 mL of DMF, and (Ph3P)2PdC12 (0.6 mmol), Cul (0.74 mmol) and triethylamine (14.3 mmol) were added. The mixture was stirred at room temperature for 1 hour, then 2.65 g (12 mmol) of 4-hydroxy-3-iodopyridine from step 37a was added. The reaction mixture was stirred at 60 C for 16 hours. The solution was cooled, diluted with toluene, and the volatiles removed in vacuo. The residue was dissolved in 1 N aqueous HCI, and this solution was washed with ether. The acidic solution was adjusted to a pH 10 with K2C03, and this solution was extracted with CH2C12. The CH2C12 extract was washed with 20%
NaOH, dried over Mg04, and evaporated. The residue was chromatographed on silica gel, eluting with 100:0 to 95:5 hexane:EtOAc to give 1.64 g (59 %) of title compound: 1H NMR
(CDC13, 300 MHz) S 1.30-1.50 (m, 9H), 1.90-2.20 (m, 4H), 2.95-3.15 (m, 2H), 5.05 (m, 1H), 6.55 (br s, 1H) 7.38 (d, 1H, J=8 Hz), 8.45 (br s, 1H), 8.85 (br s, 1H); MS m/z:
289 (M+H)+.

SUBSTITUTE SHEET (RULE 26) 37c 2-(1-methvl-2-(S)-pyLrolidinyl)furof3,2-clpvridine dihvdrochloride A 580 mg sample of the compound from step 37b above was dissolved in an aqueous solution of 37% formaldehyde (8 mL) and 88% formic acid (4 mL) and heated at reflux for 1 hour. The solution was cooled, diluted with water, and adjusted to pH 10 with K2CO3. The mixture was extracted with CH2C12, and the extract dried and concentrated.
The residue was purified by chromatography on silica gel, eluting with 100:0 to 97:3 CHC13:MeOH. The product was dissolved in ethanol at ambient temperature and a solution of hydrochloric acid in Et20 was added dropwise. The resultant white precipitate was then collected by evaporation of solvent and triturated with three portions of Et20 to give the title compound (552 mg, 70 %): 1 H NMR (D20, 300 MHz) 82.20 (br s 2H), 2.38-2.57 (m, 3H), 2.85 (br s, 3H), 3.26 (br s, 1H), 3. 85 (br s, 1H), 7.44 (s, 1H), 7.98 (d, 1H, J=6.8 Hz), 8.56 (d, 1H, J=2.3 Hz), 9.10 (s, 1H, ); MS m/z; 203 (M+H)+; Anal. Calcd for C 12H 14N20=2.0 HCl=0.2 H20=0.2 ethanol: C, 51.72; H, 6.16 N, 9.73. Found: C, 51.86;
H, 6.13; N, 9.54.
ExaIDule 38 &e~
N CI
I \

C / CI

Preparation of 2-(Hexahydro-lH-7a-pvrrolizinvl)-5.6-dichlorofurof3.2-blp rny 'dine hydrochloride 38a. 5.6-Dichloro-2-(hexahydro-1 H-7a-Rvrrolizinvl)furo f 3.2-blpyridine 2,3-Dichloro-6-iodo-5-pyridinol (163 mg, 0.56 mmol) from example 25a, copper(I) iodide (20 mg, 0.10 mmol), bis(triphenylphosphine)palladium(II) chloride (20 mg, 0.030 mmol) and triethylamine (176 mL, 0.67 mmol) were combined and allowed to stir for 1 hour at ambient temperature. 7a-ethynyl-hexahydro-lH-pyrrolizine (91 mg, 0.67 mmol) in DMF (1.0 mL) was added to the reaction mixture which was then heated to 60 C
for 18 hours. After cooling to ambient temperature, 2 N aqueous HCl was added and the mixture was washed with Et20 (2X), basified with 15% NaOH solution and extracted with (2X). The CH2C12 phases were combined, dried (MgSO4), concentrated and the residue was chromatographed (silica gel; EtOAc/hexane, 1:3) to afford a white solid (116 mg, 70%):
1H NMR (CDC13, 300 MHz) S 1.83-1.97 (m, 6H), 2.20-2.31 (m, 2H), 2.67-2.77 (m, 2H), 3.18-3.25 (m, 2H), 6.71 (s, 1H), 7.78 (s, 1H); MS (CI/NH3) m/z: 297 (M+H)+.
38b. 5 6-Dichloro-2-(hexahydro-lH-7a-gyrrolizinyl)furo[3 2-bll2vridine hydrochloride 5,6-Dichloro-2-(hexahydro-1 H-7a-pyrrolizinyl)furo[3,2-b]pyridine (108 mg, 0.36 mmol) was dissolved in Et20 (7 mL) and a saturated solution of HCl in Et20 was added SUBSTITUTE SHEET (RULE 26) dropwise. The solvent was removed and the product was recrystallized from MeOH/Et20 to afford a white solid (88.5 mg, 74%): mp 229-231 C; 1 H NMR (D20, 300 MHz) S
2.28-2.95 (m, 6H), 2.75-2.83 (m, 2H), 3.35-3.45 (m, 2H), 3.75-3.83 (m, 2H), 7.27 (s, 1H), 8.24 (s, 1H); MS (CI/NH3) m/z: 297 (M+H)+; Anal. Calcd for C14H14C12N20=1.5 HCI=0.5 H20: C, 46.60; H, 4.61; N, 7.76. Found: C, 46.74; H, 5.00; N, 7.67.
.xam lp e 39 Preparation of 5.6-Dichloro-2-(2-(R)-p olidinvl)furo[3.2-blpyridine hydrochloride 39a. 5.6-Dichloro-2-(1-t-butvloxycarbonyl-2-(R)-pyrrolidinvl)furof3.2-blp, riy dine 5,6-Dichloro-2-iodo-3-pyridinol (632 mg, 2.2 mmol), copper(I) iodide (75 mg, 0.40 mmol), bis(triphenylphosphine)palladium(II) chloride (77 mg, 0.11 mmol) and triethylamine (370 mL, 2.6 mmol) were combined in DMF (2.7 mI,) and allowed to stir for 1 hour. 1-t-Butyloxycarbonyl-2-(R)-ethynylpyrrolidine (510 mg, 2.6 mmol) in DMF (1 mL) was added and the mixture was heated to 60 C for 16 hours. After cooling to ambient temperature, the mixture was poured over Et20/saturated K2C03 solution and the phases were separated. The organic phase was washed with brine:water (1:1) (4X), dried (MgSO4) and concentrated. The residue was chromatographed (silica gel; EtOAc/hexane, 1:6) to afford an amber oil (365 mg, 46%): 1 H NMR (CDC13, 300 MHz) 8 1.32 and 1.45 (two br s, 9H), 1.95-2.40 (m, 4H), 3.45-3.74 (m, 2H), 4.92-5.13 (m, 1H), 6.62 (s, 1H), 7.81 (s, IH); MS (CI/NH3) m/z: 357 (M+H)+.
39b. 5.6-Dichloro-2-(2-(R)-pyrrolidinYl)furof 3.2-blpyridine 5,6-Dichloro-2-(1-t-butyloxycarbonyl-2-(R)-pyrrolidinyl)furo[3,2-b]pyridine (355 mg, 1.0 mmol) was dissolved in CH2C12 (3 mL) and TFA (3 mL) was added at ambient temperature. After stirring for 1 hour, the solvent was removed and the residue was redissolved in CH2C12 and washed with saturated K2C03 solution, dried (MgSO4) and concentrated. The crude product was chromatographed (silica gel; CHC13/MeOH, 98:2) to afford a solid (220 mg, 87%): IH NMR (CDC13, 300 MHz) S 1.81-2.05 (m, 3H), 2.15-2.29 (m, 1 H), 3.04-3.20 (m, 2H), 4.39-4.42 (m, 1 H), 6.70 (s, 1 H), 7.80 (s, 1 H); MS
(CI/NH3) m/z: 257 (M+H)+.
39c. 5.6-Dichloro-2-(2-(R)-pyrrolidinvl)furof 3.2-b]pvridine hydrochloride 5,6-Dichloro-2-(2-(R)-pyrrolidinyl)furo[3,2-b]pyridine (120 mg, 0.47 nunol) was slurried in Et20 (5 mL) and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed and the product was recrystallized from MeOH/Et20 to afford short white needles (86 mg, 63%): mp >260 C; [a]p20 -4.5 (c 0.51, MeOH); 1H NMR
(D20, 300 MHz) S 2.18-2.65 (m, 4H), 3.51-3.56 (m, 2H), 5.05 (dd, J=8, 8 Hz, 1H), 7.16 (d, SUBSTITUTE SHEET (RULE 26) J=1 Hz, 1H), 8.24 (d, J=1 Hz, 1H); MS (CI/NH3) m/z: 257 (M+H)+; Anal. Calcd for C 11 H l OC12N2O=HCI: C, 45.00; H, 3.78; N, 9.54. Found: C, 45.01; H, 3.71; N, 9.48.

Examlile 40 Preparation of 5.6-Dichloro-2-(1-methvl-2-(R)-pvrrolidinvl)furof3.2-blpyridine hydrochloride 5,6-Dichloro-2-(2-(R)-pyrrolidinyl)furo[3,2-b]pyridine from Example 39b (56 mg, 0.22 mmol) was dissolved in an aqueous solution of 37% formaldehyde (excess) and 88%
formic acid (excess). The aqueous mixture was heated to 60 C for 1 hour and then allowed to cool to ambient temperature. The reaction mixture was washed with Et20, basified with 15% NaOH solution and extracted with CH2C12 (2X). The organic phases were combined, dried (MgSO4), concentrated and chromatographed (silica gel; CHC13/MeOH, 98:2) to afford a solid. The solid was dissolved in Et20 (10 mL) and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed and the product recrystallized from MeOH/Et20 to afford a white solid (31 mg, 46%): mp 244-246 C; IH NMR (D20, MHz) S 2.27-2.37 (m, 2H), 2.47-2.71 (m, 2H), 2.93 (s, 3H), 3.38 (m, 1H), 3.78 (m, 1H), 4.81 (m, partially buried under H20 peak, IH), 7.27 (s, 1H), 8.26 (s, 1H); MS
(CI/NH3) m/z: 271 (M + H)+; Anal. Calcd for C12H12C12N20=HCI: C, 46.85; H, 4.26;
N, 9.11. Found: C, 46.53; H, 4.21; N, 8.82.
Examnle 41 N_ O
H H

Preparation of 2-((1R.4S)-2-aza-3-(S)-bicycloi2.2.llheptyl)-furof3.2-blQ
'vndine dihydrochloride 41a. Ethyl (lR.4S)-3-(S)-2-azabicvclo(2.2.llheptanecarboxvlate hydrochloride A suspension of ethyl (1S,4R)-3-(S)-N-((R)-a-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-enecarboxylate (2.40 g, 8.80 mmol, prepared according to the method described by L. Stella et al., Tetrahedraa Lett., 31:2603 (1990)) in ethariol (100 mL) and 20% Pd/C (1.2 g) was placed under 4 atmosphere of H2 at room temperature for 12 hours. The reaction mixture was then filtered and concentrated in vacuo to give the free base as an oil (1.33 g). 1H NMR (CDC13, 300 MHz) S 4.18 (q, 2H), 3.57 (br s, 1H), 3.34 (s, IH), 2.63 (br s, 1H), 2.12 (m, 2H), 1.68-1.28 (m, 5H), 1.28 (t, 3H); MS
(CI/NH3) SUBSTITUTE SHEET (RULE 26) m/z: 170 (M+H)+. The resultant oil was dissolved in CH202 (- 20 mL) and upon addition of HCl/Et2O (-6.25 M) a white solid precipitated. The solid was then recrystallized from EtOH/Et20 and dried under vacuum at 50 C to give the title compound (0.94 g, 52%): mp >200 C.
41 b. Ethyl (1R.4S )-N-BOC-2-aza-3-(S)-bicyclof 2.2.11 heptanecarboxvlate To a solution of the compound of example 41 a (5.0 g, 24.4 mmol) in CH2C12 (100 mL) at room temperature under nitrogen was added NEt3 (3.4 g, 24.4 mmol) followed by di-t-butyldicarbonate (5.8 g, 26.8 mmol). After 18 hours aqueous pH 4 buffer was added and the mixture was extracted with Et20 (2 x 50 mL). The combined organic layers were dried over anhydrous sodium sulfate and concentrated in vacuo. The crude product was purified by flash chromatography on silica gel (EtOAc/hexane 1:4) to yield the title compound (5.4 g, 82%) as an oil: IH NMR (CDC13, 300 MHz) S 1.28 (br d, 1H), 4.18 (m, 2H), 3.78 (d, 1H), 2.67 (br. s, 1H), 1.94 (br d, 1H), 1.80-1.40 (m, 5H), 1.44 (d, 9H), 1.28 (t, 3H); MS (CI/NH3) m/z: 270 (M+H)+, 287 (M+NH4)+
41c. (1R.4S)-3-(S)--N-BOC-2-azabicyclo(2.2.1lheptanemethanol To a solution of the product of example 41 b (20.0 g, 74.3 mmol) in THF (100 mL) at 0 C under nitrogen was added lithium aluminum hydride (5.64 g, 148.5 mmol) slowly.
The mixture was stirred for 1.5 hours and then quenched with Na2SO4 = 10H20.
Et20 was added and the mixture was stirred for 1 h, filtered through diatomaceous earth and concentrated in vacuo to give the title compound (16.9 g, 100%) as a white solid: 1H NMR
(CDC13, 300 MHz) S 1.25 (d, J=10.5 Hz, 1H), 1.49 (s, 9H), 1.58-1.78 (m, 4H), 2.30 (br d, J=1.8 Hz, 1H), 3.43-3.63 (m, 4H), 4.10 (s, 1H), 4.43 (dd, J=2.4, 2.4 Hz, 1H); MS
(CI/NH3) m/z: 171 (M-t-butyl+H)+, 228 (M+H)+.
41d. (1R,4S)-N-BOC-2-aza-3-(S)-bicvclof2.2.11heptanecarboxaldehyde To a mixture of the product of example 41c in DMSO (70 mL) was added a solution of sulfur trioxide pyridine complex (17.63 g, 110.7 mmol) in DMSO (30 mL). The mixture was then stirred for 15 minutes, poured into ice water, and extracted with Et20. The organic layer was then washed with saturated NaHCO3, 10% citric acid, H20, and brine, dried (MgSO4), and concentrated in vacuo to give the title compound as an oil (5.08 g, 60%): 1H NMR (CDC13, 300 MHz) 6 1.26 (m, 1H), 1.45 (s, 9H), 1.61-1.81 (m, 5H), 2.75 (s, 1H), 3.66 (s, 1H), 4.31 (s, 1H), 9.55 (d, J=2.1 Hz, 1H); MS (CI/NH3) m/z: 226 (M+H)+, 243 (M+NH4)+
41 e. (1R ,4S)-3-(S)-(2,2-Dibromoethenyl)-N-BOC-2-azabickclof 2.2.1lheptane To a solution of triphenylphosphine (29.6 g, 113 mmol) in CH202 (60 mL) under nitrogen at 0 C was added carbon tetrabromide (14.9 g, 45.2 mmol). The mixture was warmed to room temperature and added slowly a solution of the product of example 41 d SUBSTtTUTE SHEET (RULE 26) (5.08 g, 22.5 mmol) in CH2CI2 (10 mL). After 5 minutes, the mixture was diluted with Et20 (50 mL) then filtered through silica gel (EtOAc wash). The filtrate was concentrated and the residue was diluted with EtOAc/hexane (1:4). The resulting precipitate was removed by filtration and the filtrate was concentrated. The resulting residue (9.77 g) was 5 chromatographed (silica gel; Hexane/Et20, 95:5; Hexane/EtOAc, 90:10) to afford a solid (4.33 g, 51%): 1H NMR (CDC13, 300 MHz) S 1.32 (br s, 1H), 1.45 (s, 9H), 1.60-1.80 (m, 5H), 2.45 (br s, 1H), 3.83 (d, J=8.1 Hz, 1H), 4.12 (br s, 1H), 6.31 (d, J=8.1 Hz, 1H); MS (CI/NH3): 382 (M+H)+.
41f. (1R.4S)- -(S)-ethvnyl-N-BOC-2-azabicvclof2.2.llheptane 10 A 2.5 M solution of n-BuLi in hexane (11.4 mL, 28.4 mmol) was added slowly to a solution of the product of Example 4le (4.33 g, 11.4 mmol) in THF (40 mL) under nitrogen at 0 C. The mixture was then stirred for 10 minutes, quenched with saturated NaHCO3 and extracted with EtOAc (2X). The combined organic extracts were washed with H20 and brine, dried (MgSO4), and concentrated. The crude oil (2.87 g) was chromatographed 15 (silica gel; Hexane/EtOAc, 90:10) to afford a colorless oil (1.17 g, 46%):
I H NMR
(CDC13, 300 MHz) S 1.36-1.42 (m, 3H), 1.50 (s, 9H), 1.66-1.75 (m, 2H), 2.10 (m, 1H), 2.25 (d, J=1.5 Hz, 1H), 2.59 (s, 1H), 3.89 (s, 1H), 4.18 (s, 1H); MS (CI/NH3):

(M+H)+, 239 (M+NH4)+.
412. 2-((1R.4S)-2-aza-3-(S)-bicyclof 2.2. l lheptyl)-furof 3.2-bl12,yridine dihydrochloride 20 A solution of 2-iodo-3-hydroxypyridine (0.4 g, 1.8 mmol), bis(triphenylphosphine)-palladium(II)chloride (0.06 g, 0.09 mmol), copper (I) iodide (0.05 g, 0.27 mmol), and NEt3 (0.25 mL, 1.8 mmol) in DMF (2 mL) was stirred for 1 hour.
Then a solution of the product of example 41f (0.4 g, 1.8 mmol) in DMF (0.5 mL) was added. The mixture was heated at 60 C for 16, quenched with saturated NaHCO3, and 25 extracted with EtOAc (2X). The combined EtOAc extracts were washed with H20 and brine, dried (MgSO4), and concentrated. The crude solid (0.64 g) was chromatographed (silica gel; hexane/EtOAc, 60:40) to give a yellow colored solid (0.27 g).
This was dissolved in CH2CI2 and upon addition of HCl/Et2O a solid precipitated which was collected and further purified by heating in MeOH with activated carbon for 15 minutes.
30 After filtering, the MeOH filtrate was concentrated to give the title compound (0.11 g, 22%) as a white solid: mp 182-185 C; [a]D23 +33.2 (c 0.29, MeOH); IH NMR (MeOH-d4, MHz) S 1.81-2.01 (m, 6H), 2.24-2.28 (br d, J=11.8 Hz, IH), 3.31 (s, 1 H), 4.28 (s, 1 H), 7.51 (s, 1H), 7.87 (m, 1H), 8.58 (br d, J=8.5 Hz, 1H), 8.62 (br s, 1H); MS
(CI/NH3) m/z: 215 (M+H)+, 232 (M+NH4)+; Anal. Calcd for C13H16C12N2O=0.2 HCI=0.5 H20:
35 C, 51.45; H, 5.71; N, 9.23. Found: C, 51.48; H, 5.72; N, 8.98.
SUBSTITUTE SHEET (RULE 26) Exa ple 42 N
A !
O
Me 'H
Preparation of 2-((1R.4 )-2-aza-3-(S)-bicvclof2.2.1lheptyl)-2-methylfuro[3.2-blpyridine dihXdrochloride To a solution of 2-((1R,4S)-2-aza-3-(S)-bicyclo[2.2.1]heptyl)furo[3,2-b)pyridine dihydrochloride (from Example 41, 0.08 g, 0.3 mmol) in EtOH (3.0 mL), formaldehyde (37% w/w aqueous) (5.0 mL) and HOAc (0.2 mL) was added sodium cyanoborohydride (0.08 g, 1.4 mmol). The mixture was stirred for 16 hours, quenched with saturated NaHCO3, and extracted with Et20. The organic layer was washed with H20, dried (MgSO4) and concentrated. The crude product (0.22 g) was chromatographed (silica gel;
EtOH/EtOAc, 10:90) to afford an oil (0.06 g). The oil was dissolved in CH2C12 and a solution of HCI in Et20 was added. The solvent was removed and the product was recrystallized from CH2C12JEt2O to afford the title compound as a white solid (0.09 g, 100%): mp 225 C (dec.); [aID23 +5.4 (c 0.35, MeOH); 1H NMR (MeOH-d4, 300 MHz) 1.84-2.24 (m, 5H), 2.41 (m, 1H), 3.12 (s, 3H), 3.20 (br s, 1H), 4.22 (s, 1H), 4.61 (s, IH), 7.62 (s, IH), 7.94 (dd, J=6.0, 6.0 Hz, 1 H), 8.73 (dd, J=0.9, 1.2 Hz, 1 H), 8.84 (br d, J=6.0 Hz, 1H); MS (CI/NH3) m/z: 229 (M+H)+, 246 (M+NH4)+; Anal. Calcd for C 14H 1 gC12N20=0.2 H20: C, 55.17; H, 6.08; N, 9.19. Found: C, 55.24; H, 5.76;
N, 9.05.

Examole 43 ci N_ f \ /
O
H H

Preparation of 2-((IR.4S)-2-aza-3-(S)-bicvclof2.2.1lheRtYl)-5-chloro-furo(3.2-blRyridine dihvdrochloride A solution of 6-chloro-2-iodo-3-hydroxy pyridine (0.46 g, 1.8 mmol) from step 1 lc, bis(triphenylphosphine)-palladium(II)chloride (0.06 g, 0.09 mmol), copper (I) iodine (0.05 g, 0.27 mmol), NEt3 (0.25 mL, 1.8 mmol) in DMF (2 mL) was stirred for 1 hour.
Then a solution of (1R,4S)-3-(S)-ethynyl-N-t-butylcarboxyl-2-azabicyclo[2.2.1)heptane SUBSTITUTE SHEET (RULE 26) from Example 41f above (0.40 g, 1.8 mmol) in DMF (0.5 mL) was added. The mixture was heated at 60 C for 16 h, quenched with saturated NaHCO3 and extracted with EtOAc.
The combined EtOAc extracts were washed with H20 and brine, dried (MgSO4), and concentrated. The crude product (0.68 g) was chromatographed (silica gel;
hexane/EtOAc, 80:20) to give a solid (0.57 g). The solid was dissolved in CH2C12 and a solution of HCl in Et20 was added. The solvent was removed and the product was recrystallized from EtOH/Et20 to afford the title compound as a white solid (0.47 g, 93%): mp >200 C;
[a]D23 +31.2 (c 0.29, MeOH); 1H NMR (MeOH-d4, 300 MHz) S 1.77-1.99 (m, 5H), 2.24 (m, 1H), 3.15 (s, 1H), 4.23 (s, 1H), 4.74 (s, 1H), 7.15 (s, 1H), 7.43 (d, J=9.0 Hz, 1H), 8.00 (dd, J=0.9, 0.9 Hz, 1H); MS (CI/NH3) m/z: 249 (M+H)+; Anal. Calcd for C13H14C12N20=0.1 HCI: C, 54.06; H, 4.92; N, 9.70. Found: C, 54.21; H, 4.90; N, 9.50.

Example 44 ci O\N-Me H
Preparation of 2-((1R.4S)-2-aza-3-(S)-bicYclof2.2.llheptvl)-5-chloro-2-methvlfuro[3,2-b]gyridine dihvdrochloride To a solution of 37% aqueous formaldehyde (12 mL) and 88% formic acid (6 mL) was added 2-((lR,4S)-2-aza-3-(S)-bicyclo[2.2.1]heptyl)-5-chlorofuro[3,2-b]pyridine dihydrochloride from Example 43 above (0.4 g, 1.4 mmol). The reaction solution was refluxed for 16 hours. After cooling to ambient temperature, the solution was basified to pH
12 by the addition of solid K2C03, and extracted with EtOAc. The organic extract was washed with H20, dried (MgSO4), and concentrated. The crude solid was dissolved in CH2C12 and a solution of HCl in Et20 was added. The solvent was removed and the title compound (0.03 g, 22%) was collected as a white solid: mp 197-200 C; [a]D23 +5.6 (c 0.23, MeOH); 1 H NMR (MeOH-d4, 300 MHz) S 1.82-2.21 (m, 5H), 2.33-2.38 (m, 2H), 3.08 (s, 3H), 3.13 (br s, 1H), 4.16 (s, 1H), 4.45 (s, 1H), 7.23 (s, 1H), 7.43 (d, J=8.7 Hz, 1H), 8.04 (dd, J=0.9, 1.2 Hz, 1H); MS (CI/NH3) m/z: 263 (M+H)+; Anal.
Calcd for C 14H 17C13N2O=0.2 HCl=0.9 H20: C, 46.82; H, 5.33; N, 7.80. Found: C, 46.76;
H, 5.34; N, 7.47.

SUBSTITUTE SHEET (RULE 26) --- ----------- -Exa ple 45 cl N_ CI
O

H H
Preparation of 2-((1R,4S)-2-aza-3-(S)-bicyclof2.2.I lheptyl)-5,6-dichlorofuro[3.2-blQ, 'diir~ ne dihvdrochloride A solution of 2-iodo-3-hydroxy-5,6-dichloropyridine (0.45 g, 1.6 mmol) from Example 25a, bis(triphenylphosphine)palladium(H)chloride (0.06 g, 0.08 mmol), copper (I) iodide (0.05 g, 0.24 mmol), NEt3 (0.22 mL, 1.6 mmol) in DMF (1.5 mL) was stirred for 1 hour. Then a solution (1R,4S)-3-(S)-ethynyl-N-t-butyloxycarboxyl-2-azabicyclo[2.2. 1 ]heptane from Example 41f above (0.4 g, 1.8 mmol) in DMF
(1.0 mL) was added. The mixture was heated at 60 C for 16 hours, quenched with saturated NaHCO3 and extracted with EtOAc (2X). The combined EtOAc extracts were washed with H20, brine, dried (MgSO4), and concentrated. The crude solid (0.60 g) was chromatographed (silica gel; Hexane/EtOAc, 80:20) to give a solid (0.2 g). The solid was then dissolved in CH202 and a solution of HCI in Et20 was added. The solvent was removed and the product was recrystallized from EtOH/Et2O to afford a white solid (23 mg, 5.1 %): mp >200 C; 1 H NMR (MeOH-d4, 300 MHz) S 1.74-1.99 (m, 5H), 2.20-2.25 (m, 1H), 3.15 (s, 1H), 4.23 (s, 1H), 4.74 (s, 1H), 7.19 (d, J=0.6 Hz), 8.30 (s, 1H); MS
(CI/NH3) m/z:
283 (M+H)+, 300 (M+NH4)+; Anal. Calcd for C 13H 12C12N2O=0.6 EtOH=0.8 HCI: C, 50.17; H, 4.86; N, 8.24. Found: C, 50.12; H, 4.78; N, 8.15.
Examole 46 N
N
H O

Br Preparation of 6-bromo-2-(2-(S)-pyrrolidinyl)furo(3.2-bjpvridine dihydrochloride 46a. 2-(1- BOC-2-(S)-12vrrolidinyl)-6-bromofurof3,2-blpyridine A sample of 5-bromo-3-pyridinol (2.06 g, 11.8 mmol) (which may be prepared according to Clausson-Kass, et al.,U.S. patent 4,192,946) and Na2CO3 (3.65 g, 2.1 mmol) were dissolved in H20 (25 mL). To this solution was added 12 (3.0 g, 12 mmol), and the reaction mixture was stirred overnight. The mixture was then poured slowly into 2M
SUBSTITUTE SHEET (RULE 26) aqueous HCI, and the acidity was adjusted to pH 3. The product was collected by filtration and crystallized from EtOH/ether, affording title compound (2.92 g, 83%): MS
(CI/NH3) m/e: 300 (M+H)+, 317 (M+NH4)+; 1 H NMR (CDC13, 300 MHz) S 7.25 (d, J=2 Hz, 1 H), 7.93 (d, J=2 Hz, IH).
A sample of 5-bromo-2-iodo-3-pyridinol (0.60 g, 2.0 mmol), from above, was dissolved in DMF (3 mL), and Pd(PPh3)2C12 (0.07 g, 0.1 mmol), Cul (0.077 g, 0.4 nvnol) and triethylamine (0.33 mL, 2.4 mmol) were added. The mixture was stirred under N2 at room temperature for 1 hour, then 1-BOC-2-(S)-ethynylpyrrolidine (0.429 g, 2.2 mmol), from Example lc above, dissolved in DMF (1 mL), was added carefully.
The reaction mixture was stirred at 60 C for 16 hours, then cooled to room temperature. The reaction mixture was diluted with ether, then washed with 10% NaOH and brine.
The organic extract was dried over MgSO4 and concentrated. The residue was chromatographed (silica gel; hexane/EtOAc, 5:1 to 2:1) to give the title compound (0.32 g, 43%): 1 H NMR
(CDC13, 300 MHz) S 1.32, 1.46 (2 s, 9H), 1.91-2.40 (m, 4H), 3.37-3.70 (m, 2H), 4.93-is 5.15 (m, 1 H), 6.66 (s, 1 H), 7.85 (s, 1 H), 8.55 (s, 1 H); MS (CI/NH3) m/z: 367, 369 (M+H)+.
46b. 6-bromo-2-(2-(S)- vrrolidinvl)furo[3 2-blpYridine dihydrochloride A sample (0.14 g, 0.38 mmol) of the compound from step 46a above was dissolved in a solution of hydrogen chloride in dioxane (4 N, 3 mL) and cooled to 0 C.
After stirring at room temperature for 16 hours, the solvent was evaporated under reduced pressure. The residue was then triturated with ether several times to give the hydrochloride salt as a white solid (0.119g, 92%): [a]D23 +4.09 (c 0.45, MeOH); 1H NMR (D20, 300 MHz) 8 2.14-2.50 (m, 3H), 2.59 (m, 1H), 3.50-3.55 (m, 2H), 5.07 (t, 1H, J=7.7 Hz), 7.22 (t, J=0.7 Hz, 1H), 8.32 (dd, J=0.7, 1.8 Hz, IH), 8.66 (d, 1H, J=1.8 Hz); MS (CI/NH3) m/z: 267, 269 (M+H)+; Anal. Calcd for C l 1 H 11 N2OBr-2.2HC1: C, 38.04; H, 3.83; N, 8.07.
Found: C, 38.01; H, 3.75; N, 7.92.

ExamQle 47 Me 0 N

Br Preparation of 6-bromo-2-(1-methyl-2-(S)-R, olidinyl)fLrnf 3.2-b1R idin ihydrochloride A sample of 2-( l- BOC-2-(S)-pyrrolidinyl)-6-chlorofuro[3,2-b]pyridine (180 mg, 0.49 mmol), from Example 46a above, was dissolved in 1.5 mL of 88% formic acid and 3 mL of 37% aqueous formaldehyde and heated at 100 C for 16 hours. The reaction mixture SUBSTITUTE SHEET (RULE 26) was cooled to ambient temperature, poured into saturated aqueous K2C03, and extracted with CH2C12. The organic extract was dried over MgSO4, and the solvent was removed.
The residue was chromatographed (silica gel; EtOAc/MeOH, 10:1) to give the aniine as colorless oil (92 mg, 67%). The amine was converted to the hydrochloride salt by treatment 5 with HCI/ether, and the salt was recrystallized from EtOH/EtOAc to give the title compound (68 mg, 61%) as a white solid: IH NMR (D20, 300 MHz) S 2.20-2.40 (m, 2H), 2.46-2.80 (m, 3H), 3.00 (br s, 3H), 3.38 (m, 1H), 3.88 (m, 1H), 7.32 (s, 1H), 8.31 (dd, J=0.7, 1.8 Hz, 1H), 8.67 (d, J=1.8 Hz, 1H); MS (CI/NH3) m/e: 281 (M+H)+, 283 (M+2H)+; Anal. Calcd for C 12H I 3N2OBr= 1.8HC1: C, 41.56; H, 4.30; N, 8.08.
Found:
10 C, 41.60; H, 4.12; N, 7.89. [a]D -2.8 (c 0.20, MeOH) Fxamnle 48 N N
Me 0 cl Br Preparation of 6-bromo-5-chloro-2-(1-methyl-2-(S)-pvrrolidinyl)furof3.2-blp ivr dine dihydrochloride 48a. 2-(1-B OC-2-(S )-Qvrrolidinyl)-5-chloro-6-bromofurof 3.2-blpyridine 3-Bromo-2-chloro-5-hydroxy-6-iodopyridine (2.0 g, 6.0 mmol)(prepared by treatment of 3-bromo-2-chloro-5-hydroxypyridine (Koch & Schnatterer, Synthesis 1990, 20 499) with 12 (ibid, p. 497), palladium (II) bis(triphenylphosphine) chloride (0.21 g, 0.30 mmol), CuI (0.228 g, 1.2 mmol) and triethylamine (1.0 mL) were dissolved in DMF (8 mL). After stirring at room temperature for 1 hour, the acetylene from Example 3a (1.40 g, 7.2 mmol) was added and the resultant mixture was stirred at room temperature for 16 hours. The mixture was diluted with EtOAc, and washed with H20: brine (1:1, 3X). The 25 organic layer was dried, concentrated and chromatographed (silica gel;
hexane/EtOAc, 10:1 to 5:1) to afford the title compound as colorless oil (170 mg, 11%): 1H NMR
(CDC13, 300MHz) 8 1.31 (s, 5H), 1.46 (s, 4H), 1.8-1.95 (m, 2H), 1.96-2.23 (m, 2H), 3.32-3.58 (m, 2H), 4.15-4.41 (m, 1H), 6.60 (s, 1H), 7.95 (s, 1H); MS (CI/NH3) m/z: 403 (M+H)+.
30 48b. 6-bromo-5-chloro-2-(1-methyl-2-(S)-pvrrolidinyl)furof3.2-blpY.ridine dihYdrochloride A sample of 2-(2-(S)-pyrrolidinyl)-5-chloro-6-bromofuro[3,2-b]pyridine (170 mg, 0.42 mmol), from Example 48a above, was dissolved in 1.0 mL of 88% formic acid and 3 mL of 37% aqueous formaldehyde and heated at 100 C for 16 hour. The reaction mixture SUBSTITUTE SHEET (RULE 26) was cooled, poured into saturated aqueous K2C03, and the mixture was extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed. The residue was chromatographed (silica gel; hexane/EtOAc, 5:1 to 1:1) to give the amine as colorless oil (60 mg, 45%). The anune was converted to the hydrochloride salt by treatment with HCI/ether, and the salt was recrystallized from EtOH/EtOAc to give the title compound (50 mg, 71%): mp 250-253 C; [a]D23 -28.3 (c, 0.35, MeOH); IH NMR (D20, 300MHz) S
2.26-2.38 (m, 2H), 2.47-2.72 (m, 2H), 2.93 (s, 3H), 3.41 (m, 1H), 3.78 (m, 1H), 7.27 (s, 1H), 8.40 (s, 1H); MS (CI/NH3) m/z: 315(M+H)+; Anal. Calcd for C 12H 12N2OBrCl=HCI: C, 40.94; H,3.72; N,7.96. Found: 40.76; H, 3.76; N, 7.79.
Examnie 49 ~N/''~~~ r H
ci Br Preparation of 6-bromo-5-chloro-2-(2-(R)-pyn:olidinvl)furof3.2-biMidine hydrochloride 49a. 2-(1-BOC-2-(R)-pyrrolidiny1)-5-chloro-6-bromofurol3.2-blgyridine 5-Bromo-6-chloro-3-hydroxy-2-iodopyridine (4.0 g, 12.0 mmol) from Example 48a, palladium (II) bis(triphenylphosphine) chloride (0.42 g, 0.60 mmol), Cul (0.456 g, 2.4 mmol) and triethylamine (2.0 mL) were mixed in DMF at room temperature.
The mixture was stirred at room temperature for one hour, the acetylene of Example 3a (2.56 g, 13.2 mmol) was added. The rnixture was heated at 55 C over two nights. After cooling to room temperature, Et20 (20 mL) was added and the mixture was washed with H20: Brine (1:1, 3X). The organic layer was dried, concentrated and chromatographed (silica gel;
Hexane/EtOAc, 10:1 to 5:1) to afford the title compound as an oil (2.71 g, 56%): 1H NMR
(CDC13, 300 MHz) S 1.31, 1.46 (s, 9H), 1.95-2.06 (m, 2H), 2.06-2.20 (m, 1H), 2.20-2.35 (m, 1 H), 3.42-3.70 (m, 2H), 4.95, 5.07 (br s, I H), 6.60 (s, 1 H), 7.95 (s, 1 H); MS
(CI/NH3) m/z: 403 (M+H)+.
49b. 6-bromo-5-chloro-2-(2-(R)-pvrrolidinvl)furo[3.2-blpvridine hydrochloride To a sample of the compound from step 49a above was added a 4.0 M solution of HCI in dioxane. After stirring for 12 hours, the solvent was evaporated. The white solid was triturated with Et20 and dried under vacuum to afford the hydrochloride salt: mp >250 C; [a]D23 -4.83 (c 0.14, MeOH); I H NMR (D20, 300 MHz) S 2.20-2.50 (m, 3H), 2.5-2.65 (m, IH), 3.51 (t, J=6.9 Hz, 2H), 5.04 (t, J=18.0 Hz, 1H), 7.15 (s, 1H), 8.39 (s, SUBSTITUTE SHEET (RULE 25) 1H); MS (CI/NH3) m/z: 301 (M+H)+; Anal. Calcd for C10H11N2OC1Br=HCI: C, 39.09;
H, 3.28; N, 8.29. Found: C, 39.12; H, 3.54; N, 7.91.

ExamQie 50 ~N/''=~~
I / N
Ma 0 cl Br Preparation of 6-bromo-5-chloro-2-(1-methyl-2-(R)-pyrrolidinvl)furof 3.2-blQvridine hydrochloride 50a. 6-bromo-5-chloro-2-(1-methyl-2-(R)-pyrrolidinyl)furo(3.2-blpyridine A sample of the compound from step 49a above (0.355 g, 0.88 mmol) in 88%
formic acid (5.0 mL) and 37% aqueous formaldehyde (10 mL) was heated at 70 C
for two hours. After cooling to room temperature, the solution was basified to pH 9 with saturated aqueous NaHCO3 and extracted with CH2C12 (3X). The combined organic extracts were dried, concentrated and chromatographed (silica gel; CH2C1211VIeOH, 10:0.2 to 10:0.5) to afford an oil (0.226 g, 81%): 1H NMR (CDC13, 300 MHz) 6 1.80-1.97 (m 1H), 2.00-2.15 (m, 2H), 2.24 (m, 1H), 2.33 (s, 3H), 3.25 (m, 1H), 3.45 (m, 1H), 4.73 (m, 1H), 6.72 (s, 1H), 7.98 (s, 1H); MS (CI/NH3) m/z: 315 (M+H)+.
50b. 6-bromo-5-chloro-2-(1-methyl-2-(R)-Ayrrolidinyl)furo[3.2-blj2vridine hydrochloride To an ethereal solution of the compound from step 50a at room temperature was added a 1.0 M solution of HC1 in Et20 dropwise until precipitation ceased. The solvent was removed, and the white solid was triturated with Et20 then dried under vacuum to afford the title compound: mp 246-248 C; [aID23 +32.65 (c 0.68, MeOH); 1 H NMR (D20, 300 MHz) 6 2.25-2.40 (m, 2H), 2.46-2.70 (m, 2H), 2.94 (s, 3H), 3.40 (m, 1H), 3.81 (m, 1H), 4.83 (m, 1H), 7.27 (s, 1H), 8.40 (s, 1H); MS (CI/NH3) m/z: 315(M+H)+;
Anal.
Calcd for C 12H 12N2OBrC1= 1.1 HCl=0.3H20: C, 39.91; H, 3.82; N,7.76. Found:
40.26;
H, 4.00; N, 7.39.

SUBSTITUTE SHEET (RULE 26) ExamQle 51 N
I N
~
Me CI
Preparation of 7-chloro-2-(1-methvl-2-(S)-pvrrolidinyl)furo[3 2-b]Qvridine hydrochloride 51a. 2-(1-BOC-2-(S)-pyrrolidinvl)-7-chloro-furo[3 2-blp idine To a solution of 4-chloro-3-hydroxy-2-iodopyridine (1.04 g, 4.10 mmol) in DMF
(10 mL) was added (Ph3P)2PdC12 (0.140 g, 0.20 mmol), CuI (0.152 g, 0.80 mmol) and Et3N (0.496 g, 4.90 mmol). The mixture was stirred at room temperature for one hour. A
solution of 1-Boc-2-(S)-ethynylpyrroiidine (0.80 g, 4.10 mmol), from step Ic above, in DMF (10 mL) was added and the mixture was heated at 60 C for 16 hours. The reaction mixture was cooled to room temperature and poured into saturated NaHCO3 and washed with Et20 (4X 100m1.). The combined organic extracts were washed with brine/H2O (1/1 400mL), dried (MgSO4), and concentrated. The crude product was chromatographed (silica gel; CH202/IvIeOH, 90:10) to afford the title compound as a brown oil (0.180 g, 14%): I H NMR (CDC13, 300MHz) S 1.40 (s, 9H), 1.95-2.10 (m, 4H), 3.10-3.25 (m, 2H), 4.90-5.10 (m, 1H), 6.65 (s, 1H), 7.10 (br s, 1H), 8.38 (br s, 1H); MS
(DCI/NH3) m/z: 323 (M+H)+.
51b. 7-chloro-2-(1-methyl-2-(S)-Ryrrolidinvl)furo[3 2-b]p,vridine hydrochloride A solution of 2-(1-BOC-2-(S)-pyrrolidinyl)-7-chlorofuro[3,2-b]pyridine, from step 51 a above, in HCO2H (15.0 mL, 88%) and H2CO (15mL, 37%) was refluxed for one hour. After cooling to room temperature and the solution was acidified to pH=2.0 with 1 N
aqueous HCI and washed with Et20 (150 mL). The aqueous layer was basified with 15%
NaOH and extracted with CH2C12 (4X 400mL). The combined CH2C12 extracts were dried (MgSO4) and concentrated. The crude material was chromatographed (silica gel;
CH2C12:MeOH, 95:5) to afford the title compound as a light yellow oil (0.036 g, 15%).
The amine was dissolved in Et20 and cooled to 0 C and a saturated solution of HCl in Et20 was added until precipitation ceased. The solvent was removed and the yellow solid was placed under vacuum to afford the title compound: [a] D23 +26.24 (c 0.05, H20); IH NMR
(D20, 300 MHz) S 2.28-2.42 (m, 2H), 2.50-2.68 (m, 2H), 2.98 (s, 3H), 3.42 (br s, 1H), 3.62 (br s, 1H), 5.00 (m, 1H), 7.38 (s, 1H), 7.59 (d, J=6.0 Hz, 1H), 8.44 (d, J=6.0 Hz, 1 H); MS (DCI/NH3) m/z: 237 (M+H)+; Anal. Calcd for C 12H 13N20= 1.2 HCl=O.lOH2O=0.20Et2O: C, 51.75; H, 5.56; N, 9.43. Found C, 51.40; H, 5.49;
N,9.03.

SUBSTiTUTE SHEET (RULE 26) F.xamole 52 N
N

O

( )-2-(7-aza-2-exo-bicxclof2.2.I 1heFtyl)furof3.2-blRvridine dihydrochloride 52a. (t)7-aza-7-(tert-butoxycarbonvl)-2-exo-bicyclo(2.2.11heFtanemethanol Boc I
N

OH
H
A solution of the exo-substituted ester (12.3 g, 48.1 mmol, prepared according to the procedure of Hernandez et al., J. Org. Chem., 60:2683-2691 (1995)) in THF
(40 mL) was added to a suspension of lithium aluminum hydride (4.38 g, 115 mmol) in THF (120 lo mL) at -10 C. After 30 minutes, the reaction was quenched by the careful addition of solid Na2SO4-10H20 until gas evolution ceased. The mixture was diluted with Et20 and some Celite*was added. The mixture was stirred at ambient temperature for 1 hour then the solids were removed by filtration through a pad of Ce1iti?and anhydrous Na2SO4.
Concentration of the filtrate afforded the title compound as a colorless oil (10.3 g, 94%):

is (CDC13, 300 MHz) S 1.23-1.55 (m, 3H), 1.45 (s, 9H), 1.75-1.82 (m, 2H), 1.88-1.94 (m, 2H), 3.38-3.44 (m, 2H), 4.14-4.22 (m, 2H); MS (CI/NH3) m/z: 228 (M+H)+, 245 ( M+NH4 )+.
52b. ( )-7-aza-7-(tert-butoxvcarbonyl)-2-exo-bicycloL2.2.llhgptanecarboxaldeh yde Boc I
N

H
~-H
20 To solution of oxalyl chloride (4.73 mL, 54.2 mmol) in CH2C12 (200 mL) at -was added dimethyl sulfoxide (7.70 mL, 108 mmol). After 10 minutes, a solution of (f)7-aza-7-(tert-butoxycarbonyl)-2-exo-(hydroxymethyl)-bicyclo[2.2.lJheptane, from step 52a above, in CH2C12 (25 mL) was added. After 15 minutes, triethylamine (31.5 mL, mmol) was added. The reaction mixture was stured at -78 C for 30 minutes, then warmed 25 to -40 C over a 30 minute period. The reaction was quenched by the addition of saturated ^
aqueous NH4CI, waimed to ambient temperature, and extracted with CH2C12 (2X).
The combined organic extracts were dried (Na2SO4) and concentrated to afford the title compound as a pale yellow oil (9.82 g, 96%): 1H NMR (CDC13, 300 MHz) S 1.42 (s, 9H), 1.39-1.60 (m, 3H), 1.84 (m, 1 H), 2.20 (m, 1 H), 2.50 (m, 1 H), 3.09 (m, 1 H), 4.29 * trade-mark SUBSTITUTE SHEET (RULE 26) (br s, 1H), 4.53 (br s, 1H), 9.64 (d, J=2.0 Hz, 1H); MS (CI/NH3) m/z: 226 (M+H)+, 243 (M+NH4)+.
52c. (t)7-aza-7-(tert-butoxvcarbonvl)-2-exo-(2.2-dibromoethenvl)-bicvcl of 2.2.1 lheptane.
Boc I
N
Br H Br 5 Carbon tetrabromide (28.9 g, 87.2 mmol) was added to a 0 C solution of triphenyphosphine (57.2 g, 218 mmol) in CH2Cl2 (200 mL) under a nitrogen atmosphere.
The solution was warmed to ambient temperature, stiured for 10 minutes, then a solution of the aldehyde from step 52b in CH2C12 (20 mL) was added via cannula. After 15 minutes, the reaction mixture was diluted with 1:1 EtOAc/hexane (300 mL) and filtered through a pad 10 of Celi4and silica gel (1:1 EtOAc/hexane wash). The filtrate was concentrated and the residue was purified by chromatography (silica gel; hexane/EtOAc 90:10) to afford the title compound as a colorless oil (12.8 g, 77%): 1H NMR (CDCI3, 300 MHz) S 1.46 (s, 9H), 1.38-1.60 (m, 3H), 1.70-1.86 (m, 3H), 2.56 (dt, J=4.4, 8.8 Hz, 1H), 4.05 (br s, 1H), 4.24 (br s, IH), 6.39 (d, J=8.8 Hz, 1H); MS (CI/NH3) m/z: 382 (M+H)+, 399 15 (M+NH4)+
52d (t)-7-(tert-butoxy onyl)-2-exo-ethynvl-7-azabicyclof2 2 llheptane Boc I
N H

H
To a solution of the vinyl dibromide (12.8 g, 33.7 mmol), from step 52c above, in THF (170 mL) at -78 C was added a 2.5 M solution of n-butyllithium in hexane (27.6 mL, 20 69.0 mmol). The reaction was quenched after 15 minutes at -78 C by the addition of saturated aqueous NH4Cl and warmed to ambient temperature. The mixture was extracted with EtOAc (2X). The combined organic extracts were washed with brine, dried (Na2SO4), and concentrated. Purification of the residue by chromatography (silica gel;
hexane/EtOAc 80:20) afforded the title compound as a colorless oil (6.95 g, 93%): 1H NMR
(CDC13, 300 25 MHz) S 1.24-1.48 (m, 2H), 1.46 (s, 9H), 1.64-1.92 (m, 4H), 2.09 (d, J=2.4 Hz, IH), 2.50 (m, 1H), 4.32 (br s, 2H); MS (CI/NH3) m/z: 222 (M+H)+, 239 (M+NH4)+.
52e. (t)-2-7-aza-(7-BOC-2-exo-bicvclo(2 2 llheptvl)furof3 2-blpyridine Boc N
~ _ N /

H
3-Hydroxypyridine (366 mg, 1.65 mmol), copper(I) iodide (47 mg, 0.25 mmol), 30 bis(triphenylphosphine)palladium(II) chloride (58 mg, 0.083 mmol) and triethylamine (242 * trade-mark SUBSTITUTE SHEET (RULE 26) WO 98/46609 PCT/[JS98/07128 mL, 1.74 mmol) were combined in DMF (3.0 mL) and allowed to stir for 1 hour. A
solution of (t)-7-(tert-Butoxycarbonyl)-2-exo-ethynyl-7-azabicyclo[2.2.1)heptane (366 mg, 1.65 mmol), from step 52d, in DMF (1 mL) was added and the reaction mixture was heated to 60 C for 12 hours then 80 C for 4 hours. After cooling to ambient temperature, the mixture was diluted with 15% NaOH and extracted with Et20 (3X). The combined organic extracts were dried (MgSO4), concentrated and purified by chromatography (silica gel;
EtOAc/hexane, 50:50) to afford the title compound as a white solid (362 mg, 70%): 1H
NMR (CDC13, 300 MHz) S 1.24 (br s, 9H), 1.38-1.64 (m, 2H), 1.79-2.01 (m, 3H), 2.13 (m, 1H), 3.15 (dd, J=5.3, 8.6 Hz, 1H), 4.42 (br s, 1H), 4.50 (br s, 1 H), 6.64 (s, 1H), 7.14 (dd, J=5.4, 8.2 Hz, 1H), 7.64 (d, J=8.2 Hz, 1H), 8.48 (d, J=5.4 Hz, 1H);
MS
(CI/NH3) m/z: 315 (M + H)+.
52f. (t)-2-(7-aza-2-exo-bicyclo[2.2.1lhgptvl)furof 3.2-bj,pvridine H N
N

O
H
The compound from step 52e above (330 mg, 1.05 mmol) was dissolved in CH202 (3 ml-) and TFA (3 mL) was added at ambient temperature. After stirring for 30 minutes, the solvent was removed and the residue was diluted with CH202 and washed with saturated K2C03 solution, dried (MgSO4) and concentrated. The crude product was chromatographed (silica gel; CHC13/MeOH/NH4OH, 90:10:0.1) to afford the amine as a light yellow oil (223 mg, 99%): 1H NMR (CDC13, 300 MHz) S 1.40-1.58 (m, 2H), 1.66-2.14 (m, 6H), 3.15 (dd, J=5.4, 9.3 Hz, 1H), 3.83 (br s, 2H), 6.60 (s, 1H), 7.16 (dd, J=5.4, 8.4 Hz, 1H), 7.64 (dd, J=1.0, 6.5 Hz, 1H), 8.48 (dd, J=1.0, 5.4 Hz, 1H); MS
(CI/NH3) m/z: 215 (M + H)+.
52g (t)-2-(7-aza-2-exo-bicyglof 2.2.1lheytvllfuroi3.2-blQ,vridine dihydrochloride H N
N /

O
H
The compound from step 52f above (219 mg, 1.02 mmol) was dissolved in Et20 and a saturated solution of HC1 in Et20 was added dropwise. The solvent was removed and the precipitate was triturated with Et20 (3X) then placed under vacuum to afford the title compound as white solid (245 mg, 80%): 1H NMR (D20, 300 MHz) S 1.85-2.32 (m, 7H), 3.77 (dd, J=5.8, 9.5 Hz, 1 H), 4.47 (m, 1H), 4.65 (d, J=3.8 Hz, 1H), 7.07 (s, 1 H), 7.64 (dd, J=5.4, 8.5 Hz, 1H), 8.30 (dd, J=1.0, 6.5 Hz, 1H), 8.55 (dd, J=1.0, 5.8 Hz, 1H); MS (CI/NH3) m/z: 215 (M+H)+, 232 (M+NH4)+; Anal. Calcd for SUBSTITUTE SHEET (RULE 26) C 13H 14N2O=2.OHC1=0.8H20: C, 51.77; H, 5.88; N, 9.29. Found: C, 51.81; H, 5.66; N, 9.07.
Examnle 53 Preparation of 2-((S)-12vrrolidinvl)-6-phenylfurof 3.2-blpyridine dihvdrochloride 53a 2-(1-Boc-2-(S)-vvrrolidinyl)-6-uhenvlfuro(3.2-b)12vridine The 2-(1-BOC-2-(S)-pyrrolidinyl)-6-bromofuro[3,2-b]pyridine (0.2g, 0.54 mmol) obtained from Example 46a above was dissolved in toluene (2 mL). Phenylbororic acid (0.19 g, 1.62 mmol), palladium (0) tetrakis(triphenyl phosphine) (40 mg) and 2 M Na2CO3 solution (1 mL) were then added to the toluene solution. The resultant mixture was heated under reflux for 16 h. The desired product was extracted with CH2C12, dried over MgSO4 and filtered. Solvent was then removed under reduced pressure and the residue was chromatographed (silica gel: Hexane/EtOAc, 5:1 to 2:1) to afford an oil (0.22 g, 100%).
t 5 MS (CI/NH3) m/z: 365 (M+H)+; IH NMR (CDC13, 300 MHz) 1.32, 1.50 (2s, 9H), 1.90-2.22 (m, 3H), 2.30 (m, 1H), 3.42-3.73 (m, 2H), 4.97-5.20 (m, IH), 6.92-7.02 (m, 1 H), 7.33-7.66 (m, 5H), 7.93 (s, I H), 8.90 (s, IH).

53b 2-(2-(S)-12yrrolidinyl)-6-phenvlfurof3.2-blp3ridine dihvdrochloride A sample of compound 53a (50 mg, 0.14 mmol) from above was dissolved in hydrogen chloride in dioxane (4 N, 3 mL) at 0 C. After stirring at room temperature for 16 h, the solvent was evaporated under reduced pressure. The residue was then triturated with ether several times to give the hydrochloride salt as a white solid (19 mg, 51%): mp 220-225 C; [a]D23 +11.5 (c 0.20, MeOH); MS (CI/NH3) m/z: 265 (M+H)+; I H NMR
(D20, 300 MHz) : 2.19-2.54 (m, 4H), 2.70 (m, IH), 3.53-3.58 (m, 2H), 5.10 (m, 1H), 7.32 (d, J=0.7 Hz, 1H), 7.50-7.63 (m, 3H), 7.75-7.78 (m, 2H), 8.46 (dd, J=0.8, 1.5 Hz, 1H), 8.87 (d, J=1.8 Hz, 1H). Anal. Calcd. for C17H16N2=2 HCl=0.2 H20: C, 60.55; H, 5.38;
N, 8.31. Found: C, 59.91; H, 5.44; N, 8.22.

ExamEle 54 Preparation of 2-(1-methvl-2-(S)-pvrroli invl)-6-phenylfuro[3.2-blpvridine dihydrochloride A sample of compound (170 mg, 0.47 mmol) from Example 53 above, was dissolved in 1 mL of HCOOH and 2 mL of HCHO and heated at 100 C for 16 hour.
The reaction mixture was cooled, poured into saturated aqueous K2C03, and the mixture was extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed.
SUBSTiTUTE SHEET (RULE 26) The residue was chromatographed (silica gel; EtOAc/MeOH, 10:1) to give the amine as colorless oil (48 mg, 40%). The amine was converted to the hydrochloride salt by treatment with HCI/ether, and the salt was recrystallized from EtOH/EtOAc to give the title compound (39 mg, 64%): mp 188-191 C; [a]D -1 (c 0.48, MeOH); MS (CIINH3) m1e: 279 (M+H)+;
1 H NMR (D20, 300 MHz) 2.32-2.36 (m, 2H), 2.52-2.74 (m, 3H), 3.02 (br s, 3H), 3.40 (m, 1H), 3.90 (m, 1H), 7.40 (s, 1H), 7.52-7.63 (m, 3H), 7.74-7.78 (m, 2H), 8.39 (dd, J=0.73, 1.84 Hz, 1H), 8.86 (d, J=1.84 Hz, 1H). Anal. Calcd. for C 1 gH 1 gN20=2.OHC1=0.5 H20: C, 60.01; H, 5.87; N, 7.78. Found: C, 59.95; H, 5.79;
N, 7.61.
Example 55 Preparation of 2-(2-(R)-pvrrolidinyl)-5-chloro-6-phenvl furof3.2-blRvridine hydrochloride 55a 2-(1-Boc-2(R)-pvrrolidinvl-5-chloro-6-phen 1 furo(3.2-b)pvridine The 2-(1-Boc-2(R)-pyrrolidinyl)-5-chloro-6-bromofuro[3,2-b]pyridine (0.495 g, 1.23 mmol) obtained in Example 50a above, was dissolved in toluene (15 mL).
Phenylbororic acid (0.18 g, 1.48 mmol), palladium (0) tetrakis(triphenylphosphine) (40 mg) and 2 M aqueous Na2CO3 (1.3 mL) were added to the reaction mixture and refluxed overnight. The solvent was evaporated and the residue was chromatographed (silica gel;
Hexane/EtOAc, 100:5 to 5:1) to afford an oil (0.41 g, 83%). MS (CI/NH3) m/z:

(M+H)+; 1H NMR (CDC13, 300 MHz) S 1.35, 1.46 (s, 9H), 1.95-2.07 (m, 2H), 2.16 (m, 1H), 2.30 (m, 1H), 3.41-3.70 (m, 2H), 4.96, 5.10 (br s, 1H), 6.67 (s, 1H), 7.46 (s, 5H), 7.68 (s, 1H).

55b 2-(2-(R)-pyrrolidinyl)-5-chloro-6-phenvl furo[3.2-blpvridine hydrochloride The compound of Example 55a was added to a solution of 4.0 M HCl in dioxane.
The mixture was stirred at room temperature overnight. The solvent was evaporated and the salt was then triturated in Et20 and dried under vacuum. mp >250 C. [a]D23-6.50 (c 0.40, MeOH); MS (CI/NH3) m/z: 300 (M+H)+; 1 H NMR (D20, 300 MHz) S 2.20-2.56 (m, 4H), 2.60 (m, 1H), 3.55 (t, J=7.5 Hz, 2H), 5.07 (t, J=8.0 Hz, 1H), 7.19 (d, J=0.7 Hz, 1H), 7.57 (s, 5H), 8.03 (d, J=1.1 Hz, 1H); Anal. Calcd. for C17H15N2OC1=1.2 HCI: C, 59.61; H, 4.77; N; 8.18. Found: C, 59.32; H, 4.67; N, 8.30.

SUBSTITUTE SHEET (RULE 26) Example 56 Preparation of 5-chloro-2-(1-methvl-2-(R)-pvrrolidinY)-6-12henvl furo13.2-bipvridine hydrochloride 56a 5-chloro-2-(1-methvl-2-(R)-12vrrolidinyl)-6-phenyl furo(3.2-b)p~,rldine To the compound obtained in Example 55a (0.22 g, 0.55 mmol) was added formic acid (3.0 mL) and formaldehyde (37%, 6 mL). The mixture was heated at 80 C
for two hours. After cooling to room temperature, the solution was basified to pH 9 with saturated aqueous NaHCO3 followed by extraction with CH2C12 (3X), The combined organic layers were dried, concentrated and chromatographed (silica gel; CH2C12/MeOH, 10:0.3 to 10:0.5) to afford an oil (0.11 g, 62%). MS (CI/NH3) m/z: 313 (M+H)+; 1 H NMR
(CDC13, 300 MHz) S 1.91 (m, 1H), 2.01-2.30 (m, 3H), 2.36 (s, 3H), 3.27 (t, J=7.6 Hz, IH), 3.46 (t, J=8.3 Hz, 1H), 4.91 (m, 1H), 6.78 (s, 1H), 7.46 (s, 5H), 7.68 (s, 1H).
56b 5-chloro-62-(1-methvl-2-(R)-pvrrolidinyl)--12henyl furo[3.2-blpvridine hydrochloride The compound obtained in Example 56a was dissolved in Et20 and 1.0 I4CI in Et20 was added dropwise. The solvent was evaporated and the salt was triturated in Et20 and dried under vacuum. mp 252-254 C.; [a]D23 +38.79 (c 0.50, MeOH); MS (CI/NH3) nVz: 313 (M+H)+; 1 H NMR (D20, 300 MHz) S 2.28-2.40 (m, 2H), 2.50-2.70 (br, 2H), 2.97 (br s, 3H), 3.40 (br s, 1 H), 3.80 (br s, 1H), 4.86 (br s, 1 H), 7.30 (s, 1 H), 7.58 (s, 5H), 8.05 (s, 1H); Anal. Calcd. for C18H17N2OC1=1.3HC1: C, 60.02; H, 5.12; N, 7.78.
Found: 60.07; 5.09; N, 7.81.

Exam ij~ e 57 Preparation of 6-(3-aminophenyl)-5-chloro-2-(2-(R)-pyrrolidinvl)furof3.2-blpyridine hydrochloride 57a 2-(1-Boc-2-(R)-12vrrolidinyl)-5-chloro-6-(3-amingphenxl) furo[3 2-b) pyndine The 2-(1-Boc-2-(R)-pyrrolidinyl)-5-chloro-6-bromo furo[3,2-b]pyridine (0.469 g, 1.2 mmol), obtained in Example 50a above, was dissolved in toluene (10 mL). 3-Aminophenyl boric acid (0.445 g, 2.87 mmol), Pd(0) tetrakis(tri-phenylphosphine) (0.04 g) and 2 M aqueous NaHCO3 (1.5 mL) were added to the solution. The mixture was refluxed for two days. The solvent was evaporated and the residue was chromatographed (silica gel;
hexane/EtOAc, 5:1 to 2:1) to afford an oil (0.20 g, 41 %). MS (CI/NH3) m/z:
414 (M+H)+;
1H NMR (CDC13, 300 MHz) S 1.34, 1.46 (s, 9H), 1.95-2.08 (m, 2H), 2.15 (m, 1H), SUBSTITUTE SHEET (RULE 26) 2.28 (m, IH), 3.58-3.68 (m, 2H), 4.98, 5.10 (br s, 1H), 6.24 (m, 1H), 6.63 (s, 1H), 6.76 (d, J=9.3 Hz, 2H), 6.82 (d, J=9.3 Hz, 1H) 7.62 (s, IH).

57b 6-(3-aminophenyl)-5-chloro-2-(2-(R)-pvrrolidinyl)-furo(3,2-blpvridine 5 The compound obtained above (0.193 g, 0.47 mmol) was dissolved in CH2C12 (2 mL) at 0 C and TFA (1 mL) was added. The mixture was stirred and warmed to room temperature. After stirring for 30 min, it was basified with saturated aqueous NaHCO3 to pH 9 and extracted with CH2C12 (3X). The combined organic layers were dried, concentrated and chromatographed (silica gel; CH2C12/1VIeOH, 10:0.5 to 10:1) to afford an 10 oil (0.125 g, 85%). MS (CI/NH3) m/z: 313 (M+H)+; 1 H NMR (CDC13, 300 MHz) S
1.99-2.05 (m, 3H), 2.23 (m, 1H), 3.70-3.82 (m, 2H), 4.43 (m, 1H), 6.73 (d, J=9.3 Hz, 2H), 6.82 (d, J=9.3 Hz, 1H), 7.28 (d, J=1.3 Hz, 2H), 7.66 (s, 1H).

57c 6-(3-aminophenyl)-5-chloro-2-(2-(R)-pyrrolidinyl)-furo[3.2-blpyridine hydrochloride i s The compound obtained above was dissolved in Et20 and 1.0 M HCI in Et20 was added dropwise. The solvent was evaporated and the salt was then triturated in Et20 and dried under vacuum. mp >250 C; MS (CI/NH3) m/z: 313 (M+H)+; 1H NMR (D20, 300 MHz) 8 2.20-2.45 (m, 3H), 2.46-2.64 (m, 1H), 3.56 (t, J=7.1 Hz, 2H), 5.06 (t, J=7.8 Hz, 1H), 6.99-7.05 (m, 3H), 7.19 (s, 1H), 7.35-7.42 (m, 1H), 8.02 (s, 1H);
Anal. Calcd.
20 for C 17H 16N30C1= 1.2HC1: C, 57.11; H, 4.85; N, 11.75. Found: C, 57.07; H, 4.87; N, 11.54.

Exam IR e 58 Preparation of 5-chloro-2-(2-(R)-pyrrolidinXl -6-Z (2-(4-p)ridylethenYl) furof3.2-blp '~ndine 25 hvdrochloride 58a 2-(1-Boc-2-(R)-12,vr1olidinXl)-5-chloro-6-(4-vinX12yridyl) furo(3.2-b)pvridine 6-bromo-5-chloro-2-(1-methyl-2-(R)-pyrrolidinyl)furo [3,2-b]pyridine (0.522 g, 1.30 mmol) from Example 50a above was dissolved in acetonitrile (10 mL). To this 30 solution was added Pd(II) acetate (0.052 g, 0.23 mmol), tri-o-tolyphosphine (0.26 g, 0.85 mmol), triethylamine (2.8 mL) and 4-vinylpyridine (0.17 mL, 1.56 mmol). The mixture was heated at 85 C for two days. EtOAc (15 mL) was added, and the mixture was washed with saturated NaHCO3. The organic layer was dried, concentrated and chromatographed (silica gel; Hexane/EtOAc, 4:1 to 1:2) to afford an oil (0.22 g, 40%). MS
(CI/NH3) m/z:
35 426 (M+H)+; 1H NMR (CDC13, 300 MHz) S 1.32, 1.46 (s, 9H), 1.96-2.05 (m, 2H), 2.15 (m, 1H), 2.28 (m, IH), 3.60 (br s, 2H), 4.98, 5.11 (br s, 1H), 6.62 (s, 1H), 6.98 (d, SUBSTiTUTE SHEET (RULE 26) J=18.3 Hz, IH), 7.42 (d, J=7.0 Hz, 2H), 7.74 (d, J=18.3 Hz, 1H), 7.99 (s, IH), 8.64 (d, J=7.0 Hz, 2H).

58b 5-chloro-6-(2-(4-pyridvlethenvl)-2-(2-(R)-pvrrolidinyl)furo(3 2-b)12,vridine The compound obtained above (0.21 g, 0.49 nunol) was dissolved in CH2C12 (2 mL) at 0 C and then TFA (1.3 mL) was added. The mixture was stirred and warmed to room temperature. After stirring for 30 min, it was basified with saturated aqueous NaHCO3 to pH 9 and extracted with CH2C12 (3X). The combined organic layer was dried, concentrated and chromatographed (silica gel; CH2CI2/MeOH, 10:0.2 to 10:0.5) to afford an oil (0.123 g, 77%). MS (CI/NH3) m/z: 326 (M+H)+; 1H NMR (CDC13, 300 MHz) S
1.80-2.10 (m, 3H), 2.26 (m, IH), 3.06-3.22 (m, 2H), 4.42(m, 1H), 6.70 (s, 1H), 6.95 (d, J=16.6 Hz, 1H), 7.42 (d, J=5.0 Hz, 2H), 7.70 (d, J=16.6 Hz, 1H), 8.00 (s, 1H), 8.61 (d, J=5.0 Hz, 2H).

58c 5-chloro-6-(2-(4-pvrid ly ethenyl)-2-(2-(R)-pyrrolidinyl)-furof 3 2-blpyridine hydrochloride The compound from step 58b above was dissolved in Et20 and 1.0 M HCI in Et20 was added dropwise. The solvent was evaporated and the salt was triturated in Et20 and dried under vacuum. mp 230 C (dec.); [aJD23 -16.25 (c 0.40, MeOH); MS (CI/NH3) m/z:

(M+H)+; 1H NMR (D20, 300 MHz) S 2.00-2.20 (m, 2H), 2.29 (m, 1H), 2.45 (m, 1H), 3.30-3.40 (m, 2H), 5.00 (m, 1H), 7.43 (s, IH), 7.65 (d, J=16.3 Hz, 1H), 7.95 (d, J=16.3 Hz, IH), 8.03 (d, J=15.8 Hz, 2H), 8.76 (s, IH), 8.79 (d, J=6.1 Hz, 2H); Anal.
Calcd. for C18H16N3OC1= 2.2HC1=0.3Et2O: C, 53.85; H, 4.99; N, 9.81. Found: C, 53.89; H, 4.85;
N,9.50.

ExamFle 59 Preparation of 5-chloro-2-(1-methvl-2-(S)-nyrrolidinvl)-6-(3-p,ridyl)furo[3 2-b]Q, idine hydrochloride 59a. 2-(1-Boc-2-(S)-12yrrolidinvl)-6-(3-pyridvl) furo (3 2-b) pyridine The 2-(1-BOC-2-(S)-pyrrolidinyl)-6-bromofuro[3,2-b]pyridine (0.24g, 0.64 mmol) obtained from Example 46a above was dissolved in toluene (6 mL). Tributyl-3-pyridyltin (0.36 g, 1.3 mmol), palladium (0) tetrakis(triphenyl phosphine) (40 mg) was then added to the toluene solution and the resultant niixture was heated under reflux for 16 h. The desired product was extracted with CH202, dried over MgSO4 and filtered. Solvent was then removed under reduced pressure and the residue was chromatographed (silica gel:
Hexane/EtOAc, 5:1 to EtOAc) to afford an oil (0.13 g, 56%). MS (CI/NH3) m/z:

SUBSTITUTE SHEET (RULE 26) (M+H)+; 1 H NMR (CDC13, 300 MHz) S 1.33, 1.55 (2s, 9H), 1.92-2.38 (m, 3H), 3.43-3.70 (m, 2H), 5.08 (m, 1H), 5.15 (m, 1H), 6.75 (brs, 1H), 7.43 (m, 1H), 7.86 (s, 1H), 7.93 (m, 1H), 8.66 (m, 1H), 8.72 (brs, 1H), 8.89 (s, 1H).

59b. 5-chloro-2-(1-methyl-2-(S)-p,yrrolidinyl)-6-(3-pyridyl)-furol3,2-blpvridine hydrochloride A sample of compound (130 mg, 0.36 mmol), from Example 59a above, was dissolved in 1 mL of HCOOH and 2 mL of HCHO and heated at 100 C for 16 hour.
The reaction mixture was cooled, poured into saturated aqueous K2C03, and the mixture was extracted with CH2C12. The extract was dried over MgSO4, and the solvent was removed.
The residue was chromatographed (silica gel; EtOAc/MeOH, 10:1) to give the amine as colorless oil (67 mg, 68%). The amine was converted to the hydrochloride salt by treatment with HCI/ether, and the salt was recrystaffized from EtOH/EtOAc to give the title compound (65 mg): mp 155-163 C; [alD +1.5 (c 0.40, MeOH); MS (CI/NH3) m/e: 280 (M+H)+;
1H NMR (D20, 300 MHz) 8 2.30-2.45 (m, 2H), 2.52-2.74 (m, 3H), 3.05 (br s, 3H), 3.40 (m, 1H), 3.92 (m, 1H), 7.47 (s, 1H), 8.23 (dd, J=6, 9 Hz, 1H), 8.50 (m, 1H), 8.86 (m, 1 H), 8.95 (m, 1 H), 8.87 (m, l H), 9.20 (m, IH). Anal. Calcd. for C 1 gH 1 gN20=3.4 HC1=H20: C, 48.43; H, 5.81; N, 10.06. Found: C, 48.46; H, 5.36; N, 9.97.
The following examples until example 133 may readily be made according to the procedures described below or as generally described, where applicable, herein. Examples 134-136 were made as described in those examples.

Examles 60 - 82 Following the procedure of Example 53, replacing the 2-(1-BOC-2(-pyrrolidinyl)-bromofuro[3,2-b]pyridine with the starting material compounds shown in Table 3 and replacing the phenylboronic acid reagent thereof with a R-B(OH)2 reagent shown in Table 3 below, the desired compounds 60-82 having R as described in Table 3 are prepared. The S
compounds or the racemic compounds may also readily be prepared from the appropriate precursor(s) as shown in the schemes or tables herein. L can be varied as well and is selected from those variables listed as R in formula I. L at the position shown in Table 3 is preferrably chosen from H, F, Cl or Me.

SUBSTITUTE SHEET (RULE 26) Table 3 (CH2)n (CH2)n ` + `0 N Br \N R
BOC ~
N H
L
L
Ex.No. n * L R of reagent R
60 2 (R) H 3-quinolinyl 3-quinolinyl 61 2 (R) H 2-naphthyl 2-naphthyl 62 2 (R) H biphenyl biphenyl 63 2 (R) H 2-thienyl 2-thienyl 64 2 (R) H 4-fluorophenyl 4-fluorophenyl 65 2 (R) H 5-pyrimidinyl 5-pyrimidinyl 66 2 (R) H 3,5- 3,5-bis(trifluoromethyl)phenyl bis(trifluoromethyl)phenyl 67 2 (R) Cl 4-chlorophenyl 4-chlorophenyl 68 2 (R) H 2,4-dichlorophenyl 2,4-dichlorophenyl 69 2 (R) H 4-methylphenyl 4-methylphenyl 70 2 (R) H 3-chloro-4-fluorophenyl 3-chloro-4-chlorophenyl 71 2 (R) H 2-formylphenyl 2-formylphenyl 72 2 (R) H 4-trifluoromethylphenyl 4-trifluoromethylphenyl 73 2 (R) H 2-hydroxy-l-naphthyl 2-hydroxy-l-naphthyl 74 2 (R) H 4'-nitro-4-biphenyl 4'-nitro-4-biphenyl 75 2 (R) H 4'-fluoro-4-biphenyl 4'-fluoro-4-biphenyl 76 2 (R) H 4'-methyl-4-biphenyl 4'-methyl-4-biphenyl 77 2 (R) H 4-methyl-3-thienyl 4-methyl-3-thienyl 78 2 (R) H 2-cyano-3-thienyl 2-cyano-3-thienyl 79 2 (R) H 2-chloro-3-thienyl 2-chloro-3-thienyl 80 2 (R) H 2,4-dimethoxy-5- 2,4-dimethoxy-5-pyrimidinyl pyrimidinyl 81 2 (R) H 5-bromo-phenyl 5-bromo-phenyl 82 2 (R) H 4-methyl-l-naphthyl 4-methyl-l-naphthyl SUBSTITUTE SHEET (RULE 26) Examnles 83-91 Following the procedure of Example 58 and replacing the 4-vinylpyridine reagent with alkene or alkyne reagent shown in Table 4 below, the desired compounds 83-91 having R as described in Table 4 are prepared The S compound or racemic compounds may also be prepared from the appropriate precursor(s). L is chosen from R as described for formula I
for the designated position and is preferrably selected from H, F, Cl or Me.
Tab (CH2)n (CH2)n N B r --~ N R
I
BOC N.~ H
L L
Ex.No. n * L R of reagent R
83 2 (R) H 1-hexyne hexynyl 84 2 (R) H ethylene vinyl 85 2 (R) H 5,5-dimethyl-1,3- 5,5-dimethyl-1,3-hexadiene hexadienyl 86 2 (R) H 5-cyano-l-pentyne 5-cyano-l-pentynyl 87 2 (R) H 5-phenyl-l-pentyne 5-phenyl-l-pentynyl 88 2 (R) H 6-hydroxyl-l-hexyne 6-hydroxyl- I -hexynyl 89 2 (R) H 2-phenyl-ethyne 2-phenyl ethynyl 90 2 (R) H 3,3-dimethylbutyne 3,3-dimethylbutynyl 91 2 (R) H 1-octyne 1-octynyl Examnle 92 2-(1-Boc-2-(R)-pvrrolidinvl)-6-cyAnofurof3.2-blFyridine hydrochloride is To a flamed dried flask purged with nitrogen is added 2-(1-Boc-2-(R)-pyrrolidinyl)-6-bromo-furo[3,2-b]pyridine, zinc cyanide and tetrakis(triphenylphosphine)-palladium(0).
To the mixture is added degassed DMF (20 mL), and the mixture is heated to 80 C for 16 hours. The mixture is poured into saturated NaHCO3 (200 mL), and extracted with EtOAc (450 mL), which is dried (MgSO4) and concentrated. The mixture is then purified by chromatography.

SUBSTITUTE SHEET (RULE 26) Fxample 93 6-benzovl-2-(2-(R)-pyrrolidinyl)furot3.2-binvridine hydrochloride 93a. 6-benzoyl-2-(1-Boc-2-(R)-pyrrolidinvl)furof 3.2-blpyridine hydrochloride 5 The 2-(1-Boc-2-(R)-pyrrolidinyl)-6-cyanofuro[3,2-b]pyridine of Example 92 in anhydrous ether at 0 C is treated with 1.5 equivalents of phenylmagnesium bromide in ether and stinring is maintained at 0 to 35 C until the nitrile is largely consumed. The solvent is evaporated and the residue is treated with 2M aqueous potassium hydrogen sulfate to hydrolyze the intermediate imine. The solution is made basic with potassium carbonate and 10 extracted with EtOAc. The combined extracts are dried (Na2SO4) and concentrated to a residue which is chromatographed (silica gel) to afford the title compound.
93.b 6-benzovl-2-(2-(R)-pyrrolidinyl)furo(3.2-bin3ridine hydrochloride 6-(benzoyl)-2-(1-Boc-2-(R)-pyrrolidinyl)furo[3,2-b]pyridine is dissolved in CH2C12 (10 mL). The mixture is cooled to 0 C, TFA (10 mL) is added and the reaction is 15 stirred for 45 minutes as it warms to room temperature. The mixture is concentrated in vacuo and taken up in a minimum amount of H20. The aqueous nzixture is basified with 15% NaOH and extracted with CH2C12 (200 mL), which is dried (MgSO4) and concentrated. The residue is chromatographed (silica gel) to afford the free amine. The isolated free amine is taken up in a minimum amount of Et20, cooled to 0 C, and treated 20 with HCl in EtOH to afford the hydrochloride salt.
ExamQles 94- 97 Following the procedure of Example 93, replacing the 2-(1-Boc-2-(R)-pyrrolidinyl)-25 6-cyanofuro[3,2-b]pyridine with the starting material compounds shown in Table 5 and replacing the phenylmagnesium bromide reagent thereof with a R5-Mg-Br Grignard reagent shown in Table 5 below, the desired compounds 94-97 having L and R5 as described in Table 5 are prepared. S or R or racemic compounds may be prepared from the appropriate precursor(s). L is equal to R as described for formula I at that position. R5 is also selected 30 from the variables as listed in formula I.

SUBSTiTUTE SHEET (RULE 26) Table 5 (CH2)n (CH2)n O < O
N C N --~- N
R N R~ N R5 Ex.No. n * L R5 of reagent R5 94 2 (R) H n-hexyl n-hexyl 95 2 (R) H 3-quinolinyl 3-quinolinyl 96 2 (R) H 2-naphthyl 2-naphthyl 97 2 (R) H 4-methyl-l-naphthyl 4-methyl-l-naphthyl Examples 98 - 103 Following the procedure of Example 93, replacing the 2-(1-Boc-2-(R)-pyrrolidinyl)-6-cyano-furo[3,2-b]pyridine with the starting material compounds shown in Table 6 and replacing the phenylmagnesium bromide reagent thereof with a R5-Mg-Br Grignard reagent shown in Table 6 below, the desired compounds 112 - 117 having L and R5 as described in Table 6 are prepared.

SUBSTiTUTE SHEET (RULE 26) Table 6 (CH2)n (CH2)n < ~ 0 < 0 0 N C N IN N

L L
Ex.No. n * L R5 of Grignard agent R5 98 2 (R) H 3-pyridinyl 3-pyridinyl 99 2 (R) H 5-pyrimidinyl 5-pyrimidinyl 100 2 (R) H 3-pyridazinyl 3-pyridazinyl 101 2 (R) H 2-thienyl 2-thienyl 102 2 (R) H phenylmethyl phenylmethyl 103 2 (S) H 2-(4-methoxy- 2-(4-methoxy-phenyl)ethyl phenyl)ethyl Examples 104 - 107 Following the procedure of Example 58, replacing 6-bromo-5-chloro-2-(I-methyl-(R)-pyrrolidinyl)furo[3,2-b]pyridine with starting material compounds shown in Table 7, replacing the 4-vinylpyridine starting reagent thereof with the starting reagent compounds shown in Table 7, then hydrogenating the product thereof with palladium or platinum on charcoal the desired compounds 104 - 107 having L and R9 as described in Table 7 are prepared. S, R or raceniic compounds may be prepared from the appropriate precursor(s).
L is chosen from R in formula I and is preferrably chosen from H, Cl, F or Me.
This reaction is partially described in Scheme 19.

SUBSTITUTE SHEET (RULE 26) Table 7 (CH2)n (CH2)n N Br N W
I
R1 N R' N
L L
Ex.No. n * L Starting reagent W
104 2 (R) H 5-carbomethoxy-3- 2-(5-carbomethoxy-vinylpyridine pyridinyl)ethyl 105 2 (R) H 5-bromo-3- 2-(5-bromo-vinylpyridine pyridinyl)ethyl 106 2 (R) H 6-amino-5-bromo-3- 2-(6-amino-5-vinylpyridine bromo-pyridinyl)ethyl 107 2 (R) H 5-bromo-6- 2-(5-bromo-6-methylamino-3- methylamino-vinylpyridine pyridinyl)ethyl Examnle 108 2-l 1-methvl-2-(R)-nyrrolidinvll-6-(5-methvl-3-pvridvl)-furo[3.2-blpyddine dihydrochloride 108a.2-(1-methvl-2-(R)-pvrrolidinyl)-6-(5-met 1-3-12)ridyll-furof3.2-blp ri~dine To a solution of 2-(1-methyl-2-(R)-pyrrolidinyl)-6-(bromo)-furo[3,2-b]pyridine from Example 47 in toluene are added (5-methyl3-pyridyl)ltributyltin and tetrakis(triphenylphosphine)palladium(0). After being refluxed overnight, the resulting mixture is cooled to room temperature. Solvent is removed, and the residue is chromatographed on a silica gel column.

SUBSTITUTE SHEET (RULE 26) 108b. 2-(1-methyl-2-(R)-pvrrolidinyl)-6-(5-methvl-3-pyiidyl)-furof 3.2-b]pvridine dihvdrochloride To a solution of 6-pyridyl-2-(1-methyl-2-(R)-pyrrolidinyl)furo[3,2-b]pyridine from step 108a in THF is added hydrogen chloride (1.0 M in Et20). A precipitate forms which is filtered, washed (Et20) and vacuum-dried to afford the hydrochloride salt.

Examnles 109-117 Following the procedure of Example 108, replacing the 2-(1-methyl-2-(R)-pyrrolidinyl)-6-(bromo)-furo[3,2-b]pyridine thereof with the starting material compound shown in Table 8 and replacing the 3-pyridinyltributyltin reagent thereof with the reagent shown in Table 8, the desired compounds 109-117 having L and R as described in Table 8 are prepared. L may also be selected from the groups listed for R of formula I
at the designated position. The heteroaryl groups (het) shown at position Y2 of formula I are added as described above using the appropriate tributyltin reagent. R or S or racemic compounds may be prepared from the appropriate precursor and are included within the scope of the invention. The reagents are either readily available or may be prepared from commercially available starting materials by standard synthetic methods.

SUBSTITUTE SHEET (RULE 26) Table 8 (CH2)n (CH2)n N Br --ON N het Me Me L L
Ex.No. n * L reagent het 109 2 (R) H (5-carbomethoxy-3- 5-carboxy-3-pyridinyl)tributyltin * pyridinyl 110 2 (R) H (5-carbomethoxy-3- 5-formyl-3-pyridinyl pyridinyl)tributyltin **
111 2 (R) H (5-hydroxymethyl-3- 5-hydroxymethyl-3-pyridinyl)tributyltin pyridinyl 112 2 (R) H (2,4-dimethoxy-5- 2,4-dimethoxy-5-pyrimidinyl)tributyltin pyrimidinyl 113 2 (R) H (2-chloro-3- 2-chloro-3-thienyl thienyl)tributyltin 114 2 (R) H (2-cyano-3- 2-cyano-3-thienyl thienyl)tributyltin 115 2 (S) H (4-methyl-3- 4-methyl-3-thienyl thienyl)tributyltin 116 2 (S) H (4-hydroxymethyl-5- 4-hydroxymethyl-5-carbomethoxy-3- carbomethoxy-3-thienyl)tributyltin thienyl 117 2 (S) H (4-methoxymethoxy- 4-methoxymethoxy-5-carbomethoxy-3- 5-carbomethoxy-3-thienyl)tributyltin thienyl *After following the procedures of Example 108, with substitutions as indicated, the carbomethoxy group is hydrolyzed with base as additional step in this preparation.
**After following the procedures of Example 108, with substitutions as indicated, the following additional steps are necessary: the carbomethoxy group is hydrolyzed with base;
SUBSTITUTE SHEET (RULE 26) the resulting free acid is reduced to the alcohol with LiAlH4, and the resulting alcohol is oxidized to the aldehyde with Swern or Collins reagents.

Examnles 118 - 120 Following the procedure of Example 58, replacing 6-bromo-5-chloro-2-(2-(R)-pyrrolidinyl)furo[3,2-b]pyridine with starting material compounds shown in Table 9, replacing the vinylpyridine starting reagent thereof with the starting reagent compounds shown Table 9, then optionally hydrogenating the product thereof with palladium on charcoal the desired compounds 118-120 having L and R9 as described in Table 9 are prepared. L may be selected from the group R of formula I as described herein for the designated position and R9, in Table 9, is chosen from ary1Cl-C6alkyl moieties as exemplified below. As with the previous compounds, the R or S or racemic compounds may be prepared from the appropriate precursor(s). In addition, as is required for all nucleophilic additions to the bicyclic ring system, L is not selected from a moiety which prevents the regioselective addition to position Y2 on the compound of formula I.
Table 9 (CH2)n (CH2)n <
N B r --~- N W
N l N
BOC ~. R
L L
Ex.No. n * L Starting reagent W
118 2 (R) H 4-methyl-3- 2-(4-methyl-3-vinylbenzene phenyl)ethyl 119 2 (R) H 4-methoxy-3- 2-(4-methoxy-3-vinylbenzene phenyl)ethyl 120 2 (R) H 4-trifluoromethyl-3- 2-(4-trifluoromethyl-vinylbenzene 3-phenyl)ethyl SUBSTITUTE SHEET (RULE 26) Examnle 121 6-benzo 1-aminomethXl(2-(2-(R)-pvrrolidinyl)furo[3.2-b]p dine hy_drochloride 121 a. 2-(1-Boc-2-(R)-pyrrolidinyl)-6-(N-aminomethvl)) furo[3.2-blpyndine 2-(1-Boc-2-(R)-pyrrolidinyl)-6-(cyano) furo[3,2-b]pyridine from step 92 is stirred in the presence of Rane~nickel and ammonium hydroxide under I atm of hydrogen at room temperature. The mixture is filtered, and the solvent is removed to give the title compound 121 b. 6-benzoylaminomethyl-2-(1-Boc-2-fRl-Qvrrolidinyl)furof3.2-blpyridine To 2-(1-Boc-2-(R)-pyrrolidinyl)-6-(N-aminomethyl)) furo[3,2-b]pyridine from step 121 a are added CH2C12, triethylamine and benzoyl chloride . The mixture is stirred at room temperature overnight, then concentrated under vacuum. The residue is chromatographed to afford the title compound.

121 c. 6-benzoylaminomethyl- 2-(1-Boc-2-(R)-p)rrolidinyl)-furof 3.2-b]pvridine hydrochloride 2-(1-Boc-2-(R)-pyrrolidinyl)-6-(N-benzoylamino)methyl)) furo[3,2-b]pyridine from step 121b was dissolved in CH202. The mixture was cooled to 0 C, TFA was added and the reaction was stiured for 45 minutes as it warmed to room temperature. The mixture was concentrated in vacuo and taken up in a minimum amount of H20. The aqueous rnixture was basified with 15% NaOH and extracted with CH2C12, which was dried (MgSO4) and concentrated. The residue was chromatographed to afford a free base. The isolated free base was taken up in a minimum amount of Et20, cooled to 0 C, and treated with HCI in EtOH to afford the hydrochloride salt. The material was dried ovemight under vacuum to afford a white solid Examnles, 122 - 127 Following the procedure of Example 121, replacing the 2-(1-Boc-2-(R)-pyrrolidinyl)-6-cyanofuro[3,2-b]pyridine starting material thereof with the starting materials shown in Table 10 below, and replacing the benzoyl chloride of step 121b with the acylating reagent shown in Table 10, the desired compounds 122-127 having L and R5 as described in Table 10 are prepared. L may be selected from R as described previously for groups at that position and R, S or racemic compounds may be prepared from the appropriate precursor. In addition, the cyano group may be further extended via carbon-carbon homologations to form extended alkyl (branched or unbranched) amines which can be further treated with X(CO)R5 or other acylating reagents or alkylating reagents to form, for example, -(Cl-C6alkyl)amide compounds within the scope of the invention. The nitrogen * trade-mark SUBSTiTUTE SHEET (RULE 26) atom on the left hand side of the molecule should be protected during the acylation process.
In Table 10, m is selected from 1-6-e.g., Cl-C6.

Table 10 (CH2)n (CH2)n - < ` O < O
N C N N m~
BOC N,,= H N N R5 L L

Ex.No. n * L Acylating agent R5 122 2 (R) H acetic anhydride acetyl 123 2 (S) H 6-chlorohexanoyl 6-chlorohexanoyl chloride 124 2 (R) Cl ethyl formate H
125 2 (S) Cl dimethyl dicarbonate methoxy 126 2 (R) H furoyl chloride furanyl 127 2 (S) H 3-nicotinoyl chloride 3-pyridyl Examoles 128-133 _ (CH2)n (CH2)n < + N O C N " N O
mA
BOC N... Rl N N R5 L L

Following the procedure of Example 121, replacing the 2-(1-Boc-2-(R)-pyrrolidinyl)-6-(cyano)-furo[3,2-b]pyridine starting material thereof with the starting materials shown in Table 11 below, and replacing the benzoyl chloride of step 121b with the acylating reagent shown in Table 11, the desired compounds 128-132 having L
and R5 as described in Table 11 are prepared. As in the previous examples, L can be selected from the R group of formula I at the designated position and R5 is selected from, for example, those alkanoyl- or benzoylating reagents as listed below or from those reagents of like kind which are known, available or readily prepared. S, R and racemic compounds may be prepared from the appropriate precursor(s). An N-alkyl or suitable protecting group is necessary on the left hand side to permit acylation.

SUBSTITUTE SHEET (RULE 26) Table 11 Ex.No. n * L Acylating agent R5 128 2 (R) H 3-phenylpropanoyl 2-phenylethyl chloride 129 2 (S) H 4-chlorobenzoyl 4-chlorophenyl chloride 130 2 (R) Cl 3-nitrobenzoyl 3-nitrophenyl chloride 131 2 (S) Cl 2-pyrrolecarboxylic 2-pyrrolyl acid + EDC
132 2 (R) H 5-nitro-2-furan- 5-nitrofuranyl carboxylic acid + EDC
133 2 (S) H 2-pyrazine-carboxylic 2-pyrazinyl acid + EDC
Substituted azacyclic compounds could be also prepared according the Schemes shown above.
The following examples were made according to the procedures described below.
Ey,ample 134 2-( 1-methyl-2-(S)-pyrrolidinvl)furof2,3-blpyridine hydrochloride The title compound was prepared from 2-(1-BOC-2-(S)-pyrrolidinyl)furo[2,3-b]pyridine, from step id above, according to the procedures of Example 12 above: H NMR
(D20, 300 MHz) S 2.35 (m, 2H), 2.60 (m, 2H), 2.95 (s, 3H), 3.35 (m, 2H), 3.81 (m, 1H), 4.83 (m, 1H), 7.24 (s, 1H), 7.46 (m, 1H) 8.22 (m, 1H), 8.36 (dd, J= 1.5, 5 Hz, 1H); MS m/z: 203 (M+H)+, 220 (M+NH4)+; Anal. Calcd for C 12H 14N20-1.6 HCI: C, i5 55.31; H, 6.03 N, 10.75. Found: C, 55.43; H, 6.09 N, 10.54.
ExamU~~ e 135.
(t)-2-(7-aza-2-exo-bicvclof2.2.llheptvl)-5-chlorofuro(3. 2-binvridine hydrochloride H cl I
N

O
H

SUBSTITUTE SHEET (RULE 26) 135a (t)-2-(7-aza-7-(tert-butoxvcarbonvl)-2-exo-bicvclof2 2 1lhevtyl)-5-chlorofurof3 2-din Boc N cl ~
N

O
H
6-Chloro-2-iodo-3-pyridinol (693 mg, 2.71 mmol) from step 11 c above, copper(I) iodide (77 mg, 0.41 mmol), bis(triphenylphosphine)palladium(II) chloride (95 mg, 0.14 mmol) and triethylamine (416 mL, 2.98 mmol) were combined in DMF (5.0 mL) and allowed to stir for 1 hour. A solution of (t)-7-(tert-butoxycarbonyl)-2-exo-ethynyl-7-azabicyclo[2.2.1]heptane (600 mg, 2.71 mmol), from step 52d above, in DMF (i mL) was added and the reaction nzixture was heated at 80 C for 14 hours. After cooling to ambient temperature, the mixture was diluted with Et20 and washed with saturated aqueous NaHCO3 and brine. The organic extract was dried (MgSO4), concentrated and purified by chromatography (silica gel; EtOAc/hexane, 20:80) to afford 596 mg of a mixture of the title compound contaminated with a minor by-product. Further purification by chromatography (silica gel; CH2C12/MeOH, 95:5) afforded the pure title compound as a white solid (296 mg, 31%): IH NMR (CDC13, 300 MHz) S 1.31 (br s, 9H), 1.45-1.65 (m, 2H), 1.85-2.00 (m, 3H), 2.10 (m, 1H), 3.13 (dd, J 5.1, 8.8 Hz, 1H), 4.41 (br s, 1H), 4.88 (br s, 1 H), 6.58 (d, J = 0.8 Hz, 1H),7.15(d,J=8.5Hz, 1H),7.60(dd,J=0.8,8.6Hz, 1H);MS
(CI/NH3) m/z: 349, 351 (M + H)+.
135b. (t)-2-(7-aza-2-exo-bicyclof2.2.11hMtvl)-5-chlorofurof3 2-blp ridine H N CI

N

O
H
The compound from step 135a above (178 mg, 0.5 10 mmol) was dissolved in 1:1 CH2C12fi'FA (4 mL) and stirred at ambient temperature -for 45 min. The solvent was removed in vacuo and the residue was diluted with saturated aqueous K2C03 and extracted with CH2C12 (3X). The combined organic extracts were dried (Na2SO4) and concentrated.
The crude product was chromatographed (silica gel; CHC13/MeOH, 90:10) to afford the title compound as a white solid (125 mg, 99%): 1 H NMR (CDC13, 300 MHz) S 1.40-1.60 (m, 2H), 1.66-2.14 (m, 5H), 3.11 (dd, J = 5.2, 8.8 Hz, 1H), 3.82-3.86 (m, 2H), 6.55 (s, IH), 7.15 (d, J = 8.5 Hz, 1H), 7.62 (dd, J = 0.9, 8.6 Hz, 1H); MS (CI/NH3) m/z: 249, 251 (M+H)+.

SUBSTITUTE SHEET (RULE 26) 135c. ( )-2-(7-aza-2-exo-bicyclof2.2.llheplyl)-5-chlorofurol3.2-b1R dine hydrochloride H CI
N
I
N

O
H
The compound from step 135b above (115 mg, 0.462 mmol) was slurried in 2:1 Et20/CH2C12 (6 mL) and a saturated solution of HCl in Et20 was added dropwise.
The solvent was removed and the precipitate was triturated with Et20 and then placed under vacuum to afford the title compound as white solid (117 mg, 89%): mp >260 C;

(D20, 300 MHz) S 1.89-2.10 (m, 4H), 2.31 (m, 1H), 2.44 (m, 1H), 3.68 (dd, J =
5.6, 9.6 Hz, 1H), 4.45 (m, 1H), 4.60 (d, J = 3.7 Hz, 1H), 6.82 (s, 1H), 7.38 (d, J =
8.8 Hz, 1H), 7.90 (d, J = 8.8 Hz, 1H); MS (CI/NH3) m/z: 249, 251 (M+H)+; Anal. Calcd for C 13H 14C1N20=HC1: C, 54.75 H, 4.95; N, 9.82. Found: C, 54.63; H, 4.85; N, 9.67.
EXamnie 136 is (t)-2-(7-aza-2-exo-bicticlof2.2.11heptyl)-5-fluorofurof3.2-blpyridine hydrochloride IH N F
` O

H
136a. 2-fluoro-5-nitrop iXr dine 2-Chloro-5-nitropyridine (100 g, 0.656 mol, Aldrich), spray-dried potassium fluoride (84.1 g, 1.45 mol, Aldrich), tetraphenylphosphonium bromide (95.3 g, 0.227 mol), and anhydrous acetonitrile (1.5 L) were combined and heated at reflux overnight.
The volume of the mixture was reduced to 750 mL and the mixture was diluted with 2 L of ether, filtered and then concentrated. The residue was triturated with hot hexane (5 x 1 L), and the combined hexane extracts were concentrated to give the title compound as a pale yellow oil (48 g, 54%):
iH NMR (CDC13, 300 MHz) S 7.14 (dd, J = 3.5, 9.0 Hz, 1H), 8.63 (m, 1H), 9.15 (d, J
1.6 Hz, 1H).

136b. 5-amino-2-fluoropyridine 2-Fluoro-5-nitropyridine from step 136a above (54.1 g, 379), 62.6 g) was combined with 10% Pd/C (1 g) in EtOH (1.4 L) and the mixture was stirred under H2 (4 atm, 3 h).
After filtration, the crude product was combined with that from a similar run (441 mmol) and chromatographed (silica gel; 100% hexane to 50:50 hexane/EtOAc gradient) to afford 74.1 g SUBSTITUTE SHEET (RULE 26) (81 %) of a solid. This material was recrystallized from EtOAc to afford the title compound (67 g, 72%): 1H NMR (DMSO-d6, 300 MHz) S 6.74 (dd, J = 3, 6 Hz, 1H), 7.11 (m, 1H), 7.26 (t, J = 1 Hz, 1H); MS (DCI/NH3) m/z: 113 (M+H)+, 130 (M+NH4 )~.

136c. 5-diazonium-2-fluoropvridine tetrafluoroborate 5-Amino-2-fluoropyridine from step 136b above (45.5 g, 406 mmol) was dissolved in DME (200 mL) and cooled to -10 C under an atmosphere of nitrogen. Boron trifluoride etherate (100 mL, 812 mmol) was added dropwise. Then a solution of tert-butyl nitrite (51.0 mL, 490 mmol) in CH2C12 (50 mL) was added at a rate which maintained the internal reaction temperature below 0 C. After 20 minutes at -10 C, pentane (250 ml) was added to aid stirring, followed by an additional portion of pentane (250 ml) after 20 minutes. The solid was collected by suction filtration, washed with pentane (2 x 250 mL) and ether (4 x 100 mL), and air dried to afford 83.6 g (98%) of the title compound which was immediately used without further purification.
136d. 5-acetoxv-2-fluoron3ridine The diazonium salt from step 136c above (83.6 g) was suspended in acetic anhydride (500 mL) and quickly warmed to 110 C 5 C until nitrogen evolution became minimal (approximately 1 hour). The solvent was removed in vacuo with a rotary evaporator (bath temperature 70 C) and the residue was diluted with Et20 (1 L) and saturated aqueous Na2CO3 (300 mL). The layers were separated and the aqueous phase was extracted with Et20 (4 x 500 mL). The combined ethereal extracts were dried (MgSO4) and concentrated.
Purification by chromatography (silica gel; hexane/EtOAc, 95:5 to 70:30) afforded the title compound (24 g, 38%): IH NMR (CDC13 300 MHz) S 2.32 (s, 3H), 6.96 (dd, J = 3, 9 Hz, 1H), 7.59 (m, 1H), 8.03 (dd, J= 0.5, 1 Hz, IH); MS (DCI/NH3) m/z: 156 (M+H)+, 171 (M+NH4)+.
136e. 2-fluoro-5-hydroxypyridine 5-Acetoxy-2-fluoropyridine (70.5 g, 454 mmol) from step 136d above was suspended in 20% aqueous NaOH (200 mL) at 0 C and stirred at ambient temperature ovemight. The solution was neutralized (pH -6) by the addition of concentrated HCI. The aqueous mixture was extracted with EtOAc (5 x 200 mL), then the combined organic extracts were dried (MgSO4), and concentrated to afford 47.9 g (93%) of a solid. The crude product was recrystallized from EtOAc to afford the title compound as a white solid (30.5 g, 59% ). The 2nd and 3rd crops were combined and recrystallized to afford an additional 9.3 g(18%) of the title compound: 1H NMR (CDC13, 300 MHz) S 6.84 (dd, J = 1.9, 5.1 Hz, 1H), 7.43 (m, 1 H), 7.81 (t, J = 2.8 Hz, 1H); MS m/z: 114 (M+H)+, 131 (M+NH4)+.

SUBSTITUTE SHEET (RULE 26) 136f. 6-fluoro-2-iodo-3-Rvridinol The title compound was prepared from 2-fluoro-5-hydroxypyridine (from step 136e above) according to the procedures of step 28d above: 1 H NMR (DMSO-d6, 300 MHz) S
7.03 (dd, J = 3.9, 8.7 Hz, IH), 7.30 (dd, J = 7.0, 8.6 Hz, 1H), 10.81 (br s, 1H); MS
m/z: 240 (M+H)+, 257 (M+NH4)+.

136g. ( )-2-(7-aza-7-(rt-butoxycarbonvl)-2-exo-bicvclof2.2.11heptvl)-5-fluorofurof3.2-b ine Boc N F
~
N

O
H
6-Fluoro-2-iodo-3-pyridinol (860 mg, 3.60 mmol) from step 136f, copper(I) iodide (103 mg, 0.540 nunol), bis(triphenylphosphine)palladium(II) chloride (126 mg, 0.180 mmol) and triethylamine (552 mL, 3.96 mmol) were combined in DMF (6 mL) and allowed to stir for 1 hour. A solution of (t)-7-(tert-butoxycarbonyl)-2-exo-ethynyl-7-azabicyclo[2.2.1Jheptane (796 mg, 3.60 mmol), from step 52d above, in DMF (1 mL) was added and the reaction mixture was heated at 80 C for 14 hours. After cooling to ambient temperature, the mixture was diluted with Et20 and washed with saturated aqueous NaHCO3 and brine. The organic extract was dried (MgSO4), concentrated and purified by chromatography (silica gel; MeOH/CH2C12, 2:98 to 5:95) to afford the title compound as a white solid (277 mg, 23%): mp 96-98 C; 1H NMR (CDC13, 300 MHz) S 1.29 (br s, 9H), 1.40-1.65 (m, 2H), 1.80-2.00 (m, 3H), 2.10 (m, 1H), 3.13 (dd, J = 5.1, 8.8 Hz, 1H), 4.42 (br s, 1 H), 4.49 (br s, 1 H), 6.55 (s, 1H), 6.76 (dd, J = 1.5, 8.7 Hz, 1 H), 7.71 (m, 1H); MS (CI/NH3) m/z: 333 (M + H)+.

136h. (f)-2-(7-aza-2-exo-bicvclof 2.2.1lhotvl)-5-fluorofurof 3.2-blpyridine H F
I N_ N

O
H
The compound from step 136g above (245 mg, 0.737 mmol) was dissolved in 1:1 CH2C12/1'FA (6 mL) and stirred at ambient temperature for 30 minutes. The solvent was removed in vacuo and the residue was partitioned between CH2C12 and saturated aqueous K2C03. The organic extract was dried (Na2SO4) and concentrated. The crude product was chromatographed (silica gel; CHC13/MeOH, 90:10) to afford the title compound as a white solid (153 mg, 89%): mp 104-106 C; 1H NMR (CDC13, 300 MHz) S 1.40-1.56 (m, 2H), SUBSTITUTE SHEET (RULE 26) 1.67-1.78 (m, 2H), 1.85 (m, 1H), 1.98 (m, 1H), 2.11 (br s, 1H), 3.10 (dd, J =
5.1, 8.8 Hz, IH), 3.83-3.86 (m, 2H), 6.52 (m, 1H), 6.75 (dd, J = 1.7, 8.8 Hz, 1H), 7.72 (ddd, J
= 0.8, 6.4, 7.3 Hz, 1H); MS (CI/NH3) m/z: 233 (M + H)+.

136i. (t)-2-(7-aza-2-exo-bicyclo(2.2. l lheptyl)-5-fluorofurof 3.2-blpyridine hydrochloride H F
N_ N

O
H
The compound from step 136h above (144 mg, 0.620 mmol) was slurried in Et20 and a saturated solution of HCl in Et20 was added dropwise. The solvent was removed and the precipitate was triturated with Et20 and then placed under vacuum to afford the title compound as white solid (160 mg, 96%): mp >260 C; 1 H NMR (D20, 300 MHz) S
1.93-2.15 (m, 4H), 2.27-2.50 (m, 2H), 3.68 (m, 1H), 4.45 (br s, 1H), 4.60 (m, 1H), 6.80 (br s, 1H), 7.00 (br d, J = 8.8 Hz, 1H), 8.03 (m, 1H); MS (CIiNH3) miz: 233 (M+H)+;
Anal. Calcd for C13H14FN2O=HCI: C, 58.11 H, 5.25; N, 10.42. Found: C, 57.95;
H, 4.95; N, 10.23.
is The preferred compounds are those designated as Examples 15, 23, 26, 55 and which are the most potent binders to the nicotinic acetylcholine receptor. The preferred use of compounds of the invention is as a nicotinic acethycholine receptor modulator as described herein. The preferred compounds, for the most part, have a chlorine at the 5-position of the moiety and, thus, the preferred class of compounds is directed thereto.

SUBSTITUTE SHEET (RULE 26)

Claims (11)

CLAIMS:

1. A compound of formula (III) or a pharmaceutically acceptable salt or prodrug thereof, wherein:
A is selected from the group consisting of:
wherein * denotes a chiral center, n is 1, 2 or 3, R1 is selected from the group consisting of H, allyl and C1-C3-alkyl;
R2 is selected from the group consisting of H, C1-C3-alkyl, C1-C3-alkoxyl, hydroxymethyl, fluoromethyl, methoxymethyl, and, R2 when substituted at a position other than alpha to the ring nitrogen atom is selected from Br, Cl, F, OH, CN, -O-CO-CH3 and -O-methanesulfonyl;

wherein R2 and n are as defined above;
wherein p and q are independently 1 or 2;

wherein p and q are independently 1 or 2;
wherein R1 is as defined above, and wherein R1 is as defined above;
R is independently selected at each occurrence from the group consisting of:
C1-C4-alkyl, vinyl, bromo, chloro, fluoro, trifluoro-C1-C4-alkyl, trichloro-C1-C4-alkyl, COOH, CO2-C1-C4-alkyl, CN, nitro, amino, hydroxy, NH-CO-C1-C3-alkyl, and NR3R3, wherein R3 is H or C1-C3-alkyl;
NR3R4, wherein R3 is H or C1-C3 alkyl and R4 is hydrogen, C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, or substituted-heteroaryl-C1-C6-alkyl-;
C(O)-R5, where R5 is hydrogen, C1-C8-alkyl, substituted-C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, substituted-heteroaryl-C1-C6-alkyl-, or O-C1-C6-alkyl-, N-R6R7, wherein R6 is selected from the group consisting of H and C1-C3-alkyl-, and R7 is selected from the group consisting of H, C1-C3-alkyl-, phenyl and substituted-phenyl;

OR8, wherein R8 is C1-C8-alkyl, phenyl, substituted-phenyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, CONR3R4; phenyl; naphthyl; substituted-phenyl; substituted-naphthyl;
biphenyl; substituted-biphenyl; heteroaryl; substituted-heteroaryl; phenyl-C1-C6-alkyl-; substituted-phenyl-C1-C6-alkyl-; heteroaryl-C1-C6-alkyl-; and substituted-heteroaryl-C1-C6-alkyl-;

wherein R9 is selected from the group consisting of hydrogen, C1-C8-alkyl, substituted-C1-C8-alkyl, phenyl, substituted-phenyl, naphthyl, substituted-naphthyl, heteroaryl, substituted-heteroaryl, phenyl-C1-C6-alkyl-, substituted-phenyl-C1-C6-alkyl-, heteroaryl-C1-C6-alkyl-, and substituted-heteroaryl-C1-C6-alkyl-;

wherein R9 is as defined above;
-CH2-NH-CO-R5, wherein R5 is as defined above; and -CH2-CH2-CO-O-C1-C6-alkyl; and m is 0 or 1, and wherein substituents in "substituted C1-C8 alkyl" hereinbefore are selected from Cl, Br, F, CN, CF3, OH, CHO, COOH, COOC1-C3 -alkyl, C1-C6-alkoxy, methoxymethoxy, amino, and C1-C3-alkyl-amino, except not more than one CHO, COOH, or COOC1-C3 -alkyl group may be present;

substituents in "substituted biphenyl" hereinbefore are selected from F, OH, NO2 and C1-C3-alkyl;

substituents in "substituted naphthyl" hereinbefore are selected from Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COOC1-C3-alkyl, C1-C3 -alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, and C1-C3 -alkyl-amino, except not more than one CHO, COOH, or COOC1-C3 -alkyl group may be present;

substituents in "substituted phenyl" and "substituted naphthyl" hereinbefore are selected from Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COOC1-C3-alkyl, C1-C3 -alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, and C1-C3 -alkyl-amino, except not more than one CHO, COOH, or COOC1-C3 -alkyl group may be present;

substituents in "substituted phenyl C1-C6 alkyl" hereinbefore are substituents on the phenyl moiety selected from Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COOC1-C3-alkyl, C1-C3-alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, and C1-C3 -alkyl-amino, except not more than one CHO, COOH, or COOC1-C3-alkyl group may be present;

substituents in "substituted heteroaryl" and "substituted heteroaryl C1-C6 alkyl"
hereinbefore are selected from Cl, Br, F, CN, CF3, NO2, OH, CHO, COOH, COOC1-C3-alkyl, C1-C3-alkyl, C1-C6-alkoxy, methoxymethyl, methoxymethoxy, amino, and C1-C3-alkyl-amino, except not more than one CHO, COOH, or COOC1-C3-alkyl group may be present.

2. The compound of formula (III), as defined in Claim 1, or a pharmaceutically acceptable salt or prodrug thereof, wherein A is selected from the group consisting of:

wherein R1, R2, n, p and q are as defined in claim 1.

3. The compound of formula (III), as defined in Claim 2, or a pharmaceutically acceptable salt or prodrug thereof, wherein R m is at the 3-position and is selected from phenyl, 4-pyridyl-2-ethenyl, pyridyl and benzoylaminomethyl

4. The compound of formula (III), as defined in Claim 1, or a pharmaceutically acceptable salt or prodrug thereof, wherein R is selected from the group consisting of Cl and C1-C4-alkyl.

5. The compound of formula (III), as defined in Claim 1, or a pharmaceutically acceptable salt or prodrug thereof, wherein m is 0

6. The compound of formula (III), as defined in Claim 1, selected from the group consisting of:
2-(1-methyl-2-(S)-pyrrolidinyl)furo(2,3-b)pyridine;
(~)-2-(7-aza-2-exo-bicyclo(2.2.1)heptyl)-5-chlorofuro(3,2-b)pyridine;
(~)-2-aza-2-exo-bicyclo(2.2.1)heptyl)-5-fluorofuro(3,2-b)pyridine;
6-phenyl-2-((S)-pyrrolidinyl)furo[3,2-b]pyridine;
6-phenyl-2-(1-methyl-2-(S)-pyrrolidinyl)furo[3,2-b]pyridine;
5-chloro-6-phenyl-2-(2-(R)-pyrrolidinyl)furo(3,2-b)pyridine;
5-chloro-2-(1-methyl-2-(R)-pyrrolidinyl)-6-phenylfuro(3,2-b)pyridine;
(3-aminophenyl)-5-chloro-2-(2-(R)-pyrrolidinyl)-6-furo(3,2-b)pyridine;
5-chloro-2-(2-(R)-pyrrolidinyl)-6-(4-vinylpyridyl)-furo(3,2-b)pyridine, and 5-chloro-2-(1-methyl-2-(S)-pyrrolidinyl)-6-(3-pyridyl)-furo(3,2-b)pyridine, or a pharmaceutically acceptable salt or prodrug thereof.

7. The compound of formula (III), as defined in Claim 2, selected from the group consisting of 6-phenyl-2-((S)-pyrrolidinyl)furo[3,2-b]pyridine;
2-(1-methyl-2-(S)-pyrrolidinyl)-6-phenylfuro[3,2-b]pyridine;
5-chloro-6-phenyl-2-(2-(R)-pyrrolidinyl)furo[3,2-b]pyridine;
5-chloro-6-phenyl-2-(1-methyl-2-(R)-pyrrolidinyl)furo[3,2-b]pyridine;
6-(3-aminophenyl)-5-chloro-2-(2-(R)-pyrrolidinyl)-furo[3,2-b]pyridine;
5-chloro-2-(2-(R)-pyrrolidinyl)-6-(4-pyridyl-2-ethenyl)furo[3,2-b]pyridine;
and 5-chloro-2-(1-methyl-2-(S)-pyrrolidinyl)-6-(3-pyridyl)-furo[3,2-b]pyridine or a pharmaceutically acceptable salt or prodrug thereof.

8. A pharmaceutical composition comprising a compound of formula (III), as defined in any one of claims 1 to 7, or a pharmaceutically acceptable salt or prodrug thereof, in combination with a pharmaceutically acceptable carrier.

9. Use of a compound of formula (III), as defined in any one of claims 1 to 7, or a pharmaceutically acceptable salt or prodrug thereof, in the manufacture of a medicament for modulating chemical synaptic transmission in a mammal.

10. A compound of formula (III), as defined in any one of claims 1 to 7, or a pharmaceutically acceptable salt or prodrug thereof, for use in modulating chemical synaptic transmission in a mammal.

11. A pharmaceutical composition, for use in modulating chemical synaptic transmission in a mammal, comprising a pharmaceutically effective amount of a compound of formula (III), as defined in any one of claims 1 to 7, or a pharmaceutically acceptable salt or prodrug thereof, in combination with a pharmaceutically acceptable carrier.