CA2266750A1 - Procedes de detection des fragments de restriction polymorphes (rfpl) amplifies et clives - Google Patents

Procedes de detection des fragments de restriction polymorphes (rfpl) amplifies et clives Download PDF

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Publication number
CA2266750A1
CA2266750A1 CA002266750A CA2266750A CA2266750A1 CA 2266750 A1 CA2266750 A1 CA 2266750A1 CA 002266750 A CA002266750 A CA 002266750A CA 2266750 A CA2266750 A CA 2266750A CA 2266750 A1 CA2266750 A1 CA 2266750A1
Authority
CA
Canada
Prior art keywords
sequence
primer
restriction site
strand
polymorphic restriction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266750A
Other languages
English (en)
Inventor
Ronald W. Davis
Frederick Ausubel
Daphne Preuss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Hospital Corp
Leland Stanford Junior University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/715,484 external-priority patent/US6004783A/en
Application filed by Individual filed Critical Individual
Publication of CA2266750A1 publication Critical patent/CA2266750A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

La présente invention a pour objet des procédés de détection de sites de restriction polymorphes dans des acides nucléiques, ainsi que les nécessaires de mise en application de ces procédés.
CA002266750A 1996-09-18 1997-09-17 Procedes de detection des fragments de restriction polymorphes (rfpl) amplifies et clives Abandoned CA2266750A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/715,484 1996-09-18
US08/715,484 US6004783A (en) 1994-03-18 1996-09-18 Cleaved amplified RFLP detection methods
PCT/US1997/016467 WO1998012352A1 (fr) 1996-09-18 1997-09-17 Procedes de detection des fragments de restriction polymorphes (rfpl) amplifies et clives

Publications (1)

Publication Number Publication Date
CA2266750A1 true CA2266750A1 (fr) 1998-03-26

Family

ID=24874229

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266750A Abandoned CA2266750A1 (fr) 1996-09-18 1997-09-17 Procedes de detection des fragments de restriction polymorphes (rfpl) amplifies et clives

Country Status (3)

Country Link
EP (1) EP0948645A4 (fr)
CA (1) CA2266750A1 (fr)
WO (1) WO1998012352A1 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU763135B2 (en) * 1998-03-27 2003-07-17 Johnson & Johnson Research Pty. Limited Catalytic nucleic acid-based diagnostic methods
ATE274069T1 (de) * 1998-11-09 2004-09-15 Methexis Genomics N V ANALYSE VON ßAMPLIKONSß ERHALTEN DURCH RESTRIKTIONSENDONUKLEASEN
EP1157131A2 (fr) * 1999-02-22 2001-11-28 Lynx Therapeutics, Inc. Fragments d'adn polymorphes et leurs utilisations
AU2001285155A1 (en) * 2000-08-21 2002-03-04 Lynx Therapeutics, Inc. Polymorphic dna fragments and uses thereof
AU2002227055A1 (en) * 2000-11-01 2002-05-15 The General Hospital Corporation Bacterial strain typing
AUPR221400A0 (en) 2000-12-20 2001-01-25 Murdoch Childrens Research Institute, The Diagnostic assay
AU2002215696B2 (en) * 2000-12-20 2007-07-19 Murdoch Childrens Research Institute Method for detecting whether an organism is homozygous or heterozygous using labelled primers and RFLP
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775619A (en) * 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4925785A (en) * 1986-03-07 1990-05-15 Biotechnica Diagnostics, Inc. Nucleic acid hybridization assays
AU3694689A (en) * 1988-04-28 1989-11-24 Mark H. Skolnick Amplified sequence polymorphisms (asps)
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5523225A (en) * 1993-09-13 1996-06-04 Regents Of The University Of Colorado DNA sequence encoding human cystathionine β-synthase
EP0812211A4 (fr) * 1994-03-18 1998-12-16 Gen Hospital Corp Methodes de detection de polymorphismes amplifies et clives des sites de restriction

Also Published As

Publication number Publication date
EP0948645A1 (fr) 1999-10-13
WO1998012352A1 (fr) 1998-03-26
EP0948645A4 (fr) 2002-11-05

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued