CA2266423A1 - Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences - Google Patents

Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences Download PDF

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Publication number
CA2266423A1
CA2266423A1 CA002266423A CA2266423A CA2266423A1 CA 2266423 A1 CA2266423 A1 CA 2266423A1 CA 002266423 A CA002266423 A CA 002266423A CA 2266423 A CA2266423 A CA 2266423A CA 2266423 A1 CA2266423 A1 CA 2266423A1
Authority
CA
Canada
Prior art keywords
cells
viral
recombinant
viruses
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002266423A
Other languages
English (en)
Inventor
Willem P.C. Stemmer
Frederick C. Christians
Shi-Kau Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2266423A1 publication Critical patent/CA2266423A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14111—Inoviridae
    • C12N2795/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Abstract

L'invention concerne des procédés de mise au point d'acides nucléiques destinés à être utilisés dans une thérapie génique grâce à une recombinaison récursive de séquences. Beaucoup de ces procédés mettent au point des vecteurs aussi bien viraux que non viraux, afin de présenter des propriétés perfectionnées. Par exemple, des vecteurs sont mis au point pour présenter des propriétés perfectionnées de titres viraux, d'infectivité, d'expression d'un gène dans un vecteur, de spécificité de tissu, de capacité de génome viral, de rétention épisomique, de manque d'immunogénicité des vecteurs ou d'un produit d'expression de ces vecteurs, d'intégration spécifique du milieu, de stabilité accrue, ou de capacité à conférer une résistance cellulaire à une infection causée par des micro-organismes. L'invention concerne également un adénovirus-phagémide susceptible de générer un ADN à un brin supérieur à 10 kilobases comprenant un adénovirus et une origine de réplication en phage f1.
CA002266423A 1996-09-27 1997-09-26 Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences Abandoned CA2266423A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US3774296P 1996-09-27 1996-09-27
US72266096A 1996-09-27 1996-09-27
US08/722,660 1996-09-27
US60/037,742 1996-09-27
PCT/US1997/017300 WO1998013487A1 (fr) 1996-09-27 1997-09-26 Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences

Publications (1)

Publication Number Publication Date
CA2266423A1 true CA2266423A1 (fr) 1998-04-02

Family

ID=26714441

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002266423A Abandoned CA2266423A1 (fr) 1996-09-27 1997-09-26 Procedes permettant l'optimisation d'une therapie genique grace a un rearrangement et une selection recursifs de sequences

Country Status (5)

Country Link
EP (1) EP0964922A4 (fr)
JP (1) JP2001504325A (fr)
AU (1) AU4503797A (fr)
CA (1) CA2266423A1 (fr)
WO (1) WO1998013487A1 (fr)

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US6426185B1 (en) 1998-01-16 2002-07-30 Large Scale Biology Corporation Method of compiling a functional gene profile in a plant by transfecting a nucleic acid sequence of a donor plant into a different host plant in an anti-sense orientation
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Also Published As

Publication number Publication date
JP2001504325A (ja) 2001-04-03
WO1998013487A1 (fr) 1998-04-02
AU4503797A (en) 1998-04-17
EP0964922A4 (fr) 2000-10-25
EP0964922A1 (fr) 1999-12-22

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