CA2265522A1 - Vecteurs retroviraux pouvant transduire des cellules ne se divisant pas - Google Patents

Vecteurs retroviraux pouvant transduire des cellules ne se divisant pas Download PDF

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Publication number
CA2265522A1
CA2265522A1 CA002265522A CA2265522A CA2265522A1 CA 2265522 A1 CA2265522 A1 CA 2265522A1 CA 002265522 A CA002265522 A CA 002265522A CA 2265522 A CA2265522 A CA 2265522A CA 2265522 A1 CA2265522 A1 CA 2265522A1
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CA
Canada
Prior art keywords
nucleic acid
retrovirus
cell
viral
dividing cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002265522A
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English (en)
Inventor
Didier P. Trono
Philippe A. Gallay
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Salk Institute for Biological Studies
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2265522A1 publication Critical patent/CA2265522A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13045—Special targeting system for viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13051—Methods of production or purification of viral material
    • C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Selon la présente invention, on a mis au point des procédés destinés à modifier des vecteurs rétroviraux dérivés de virus qui ne sont pas connus comme étant pathogènes chez l'homme (par exemple le virus de la leucémie murine de Moloney), afin que ces vecteurs puissent transduire des séquences hétérologues dans des cellules ne se divisant pas. Ainsi, on a découvert que l'on pouvait rendre des rétrovirus capables d'infecter des cellules ne se divisant pas, en introduisant dans la particule virale l'une de ces modifications déterminées bien précisément. Par exemple, on peut associer un élément reconnu par le mécanisme d'importation nucléaire d'une cellule ne se divisant pas, au complexe de nucléoprotéines du rétrovirus. Dans un autre mode de réalisation, on modifie au moins une protéine codée par l'acide nucléique viral gag ou pol, de façon que cette protéine soit reconnue par le mécanisme d'importation nucléaire d'une cellule ne se divisant pas.
CA002265522A 1996-09-17 1997-09-08 Vecteurs retroviraux pouvant transduire des cellules ne se divisant pas Abandoned CA2265522A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US71531896A 1996-09-17 1996-09-17
US08/715,318 1996-09-17
PCT/US1997/015934 WO1998012314A1 (fr) 1996-09-17 1997-09-08 Vecteurs retroviraux pouvant transduire des cellules ne se divisant pas

Publications (1)

Publication Number Publication Date
CA2265522A1 true CA2265522A1 (fr) 1998-03-26

Family

ID=24873552

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002265522A Abandoned CA2265522A1 (fr) 1996-09-17 1997-09-08 Vecteurs retroviraux pouvant transduire des cellules ne se divisant pas

Country Status (5)

Country Link
EP (1) EP0970201A1 (fr)
JP (1) JP2001501815A (fr)
AU (1) AU4261797A (fr)
CA (1) CA2265522A1 (fr)
WO (1) WO1998012314A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
EP1076715B1 (fr) * 1998-05-13 2007-07-18 Genetix Pharmaceuticals Inc. Cellules lentivirales d'encapsidation
EP1171624B1 (fr) 1999-04-29 2007-07-25 Cell Genesys, Inc. Procede et moyens de production de vecteurs lentiviraux recombinants surs et a titre eleve
EP1103615A1 (fr) * 1999-11-25 2001-05-30 Universite De Geneve Vecteurs capables d'immortaliser les cellules ne se divisant pas et les cellules immortalisées par ces vecteurs
JP2003534787A (ja) * 2000-05-26 2003-11-25 カイロン コーポレイション レンチウイルスベクターを使用する神経細胞の形質導入の方法
DE60233047D1 (de) 2001-05-14 2009-09-03 Gbp Ip Llc Lentivirale vektoren kodierend für gerinnungsfaktoren für die gentherapie

Also Published As

Publication number Publication date
JP2001501815A (ja) 2001-02-13
AU4261797A (en) 1998-04-14
WO1998012314A1 (fr) 1998-03-26
EP0970201A1 (fr) 2000-01-12

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Legal Events

Date Code Title Description
FZDE Discontinued