CA2247798A1 - Inhibiteurs de cysteine-proteinases du soja nucleotides codant ces agents et procedes pour leur utilisation - Google Patents
Inhibiteurs de cysteine-proteinases du soja nucleotides codant ces agents et procedes pour leur utilisation Download PDFInfo
- Publication number
- CA2247798A1 CA2247798A1 CA002247798A CA2247798A CA2247798A1 CA 2247798 A1 CA2247798 A1 CA 2247798A1 CA 002247798 A CA002247798 A CA 002247798A CA 2247798 A CA2247798 A CA 2247798A CA 2247798 A1 CA2247798 A1 CA 2247798A1
- Authority
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- Canada
- Prior art keywords
- seq
- plant
- nucleotide sequence
- protein
- cysteine proteinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 239000012268 protein inhibitor Substances 0.000 description 1
- 229940121649 protein inhibitor Drugs 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000019710 soybean protein Nutrition 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
- 108010061238 threonyl-glycine Proteins 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Cette invention se rapporte à l'isolation, à la purification et à l'utilisation de trois inhibiteurs de cystéine-protéinases du soja, appelé L1, R1 et N2, qui ont un fort pouvoir inhibiteur sur les cystéine-proteinases se trouvant dans l'appareil digestif de certains insectes herbivores. Cette invention se rapporte en outre à trois séquences nucléotidiques qui ont été clonées, isolées et séquencées, ces séquences nucléotidiques codant pour ces trois protéines mentionnées, ainsi qu'un vecteur dans lequel on a introduit une ou plusieurs de ces séquences. Cette invention se rapporte également à des plantes transformées par des vecteurs faisant l'objet de cette invention, à des plantes transformées ayant une capacité accrue de résister à la prédation par ces insectes, ainsi qu'à des micro-organismes transformés au moyen de vecteurs ou de plasmides de cette invention.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1242396P | 1996-02-28 | 1996-02-28 | |
US60/012,423 | 1996-02-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2247798A1 true CA2247798A1 (fr) | 1997-09-04 |
Family
ID=21754901
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002247798A Abandoned CA2247798A1 (fr) | 1996-02-28 | 1997-02-28 | Inhibiteurs de cysteine-proteinases du soja nucleotides codant ces agents et procedes pour leur utilisation |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0964914A4 (fr) |
CA (1) | CA2247798A1 (fr) |
WO (1) | WO1997032007A1 (fr) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2824842B1 (fr) * | 2001-05-18 | 2004-04-30 | Univ Paris 7 Denis Diderot | Phytocystatine |
WO2005030967A2 (fr) | 2003-09-25 | 2005-04-07 | Pioneer Hi-Bred International, Inc. | Inhibiteurs de la cystatine proteinase de plantes de culture, et son procede d'utilisation |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9104617D0 (en) * | 1991-03-05 | 1991-04-17 | Nickerson Int Seed | Pest control |
US5317086A (en) * | 1992-03-09 | 1994-05-31 | The Regents Of The University Of California | Cysteine proteinase inhibitors and inhibitor precursors |
-
1997
- 1997-02-28 WO PCT/US1997/003234 patent/WO1997032007A1/fr not_active Application Discontinuation
- 1997-02-28 EP EP97907967A patent/EP0964914A4/fr not_active Withdrawn
- 1997-02-28 CA CA002247798A patent/CA2247798A1/fr not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP0964914A1 (fr) | 1999-12-22 |
WO1997032007A1 (fr) | 1997-09-04 |
EP0964914A4 (fr) | 2001-07-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued | ||
FZDE | Discontinued |
Effective date: 20030228 |