CA2243598A1 - Cold-active protease cp70 - Google Patents
Cold-active protease cp70Info
- Publication number
- CA2243598A1 CA2243598A1 CA002243598A CA2243598A CA2243598A1 CA 2243598 A1 CA2243598 A1 CA 2243598A1 CA 002243598 A CA002243598 A CA 002243598A CA 2243598 A CA2243598 A CA 2243598A CA 2243598 A1 CA2243598 A1 CA 2243598A1
- Authority
- CA
- Canada
- Prior art keywords
- protease
- inactivated
- temperature
- hemoglobin
- casein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 7
- 239000004365 Protease Substances 0.000 title abstract 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 title abstract 7
- 102000009027 Albumins Human genes 0.000 abstract 2
- 108010088751 Albumins Proteins 0.000 abstract 2
- 108010076119 Caseins Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 108010010803 Gelatin Proteins 0.000 abstract 2
- 102000001554 Hemoglobins Human genes 0.000 abstract 2
- 108010054147 Hemoglobins Proteins 0.000 abstract 2
- 239000005018 casein Substances 0.000 abstract 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 abstract 2
- 235000021240 caseins Nutrition 0.000 abstract 2
- 229920000159 gelatin Polymers 0.000 abstract 2
- 239000008273 gelatin Substances 0.000 abstract 2
- 235000019322 gelatine Nutrition 0.000 abstract 2
- 235000011852 gelatine desserts Nutrition 0.000 abstract 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 abstract 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
Abstract
A psychrophilic protease is here disclosed which has the following physicochemical properties: (a) specific activity and substrate specificity:
the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable at a pH in the range of 6.5 to 10.0 at 30 ~C for 1 hour; (d) optimal temperature: about 40 ~C; (e) temperature stability: at pH 7 for 1 hour, the protease is scarcely inactivated at a temperature up to 30 ~C, but it is inactivated at 40 ~C as much as about 40 % and completely inactivated at 50 ~C in about 10 minutes;
(f) enzyme activity: the protease has about 50 % or more of its maximum activity at 20 ~C; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.
the protease acts on casein, gelatin, hemoglobin and albumin to specifically decompose them in the order of casein, gelatin, hemoglobin and albumin; (b) optimal pH: 8.0; (c) pH stability: the protease is stable at a pH in the range of 6.5 to 10.0 at 30 ~C for 1 hour; (d) optimal temperature: about 40 ~C; (e) temperature stability: at pH 7 for 1 hour, the protease is scarcely inactivated at a temperature up to 30 ~C, but it is inactivated at 40 ~C as much as about 40 % and completely inactivated at 50 ~C in about 10 minutes;
(f) enzyme activity: the protease has about 50 % or more of its maximum activity at 20 ~C; (g) the active center of the enzyme is serine; and (h) the molecular weight of the protease is about 70 kDa as measured by SDS-PAGE.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8/12207 | 1996-01-26 | ||
| JP8012207A JPH09201195A (en) | 1996-01-26 | 1996-01-26 | Low-temperature active protease cp70 |
| PCT/US1997/001148 WO1997027313A1 (en) | 1996-01-26 | 1997-01-24 | Cold-active protease cp70 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2243598A1 true CA2243598A1 (en) | 1997-07-31 |
| CA2243598C CA2243598C (en) | 2002-10-22 |
Family
ID=11798951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002243598A Expired - Fee Related CA2243598C (en) | 1996-01-26 | 1997-01-24 | Cold-active protease cp70 |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0876500A4 (en) |
| JP (1) | JPH09201195A (en) |
| CN (1) | CN1214084A (en) |
| AR (1) | AR005541A1 (en) |
| BR (1) | BR9707203A (en) |
| CA (1) | CA2243598C (en) |
| WO (1) | WO1997027313A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09224666A (en) * | 1996-02-16 | 1997-09-02 | Procter & Gamble Co:The | Low temperature active protease cp58 low temperature bacterium |
| WO1999025848A1 (en) * | 1997-11-14 | 1999-05-27 | The Procter & Gamble Company | A polynucleotide encoding cp70 cold active protease |
| JP2019080493A (en) * | 2017-10-27 | 2019-05-30 | プリマハム株式会社 | Protease, detergent composition, washing method, and microorganism |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996025489A1 (en) * | 1995-02-17 | 1996-08-22 | The Procter & Gamble Company | Psychrophilic protease and psychrophilic bacteria |
| JP3592795B2 (en) * | 1995-06-01 | 2004-11-24 | 花王株式会社 | Low-temperature optimal alkaline protease, microorganism producing the same, and method for producing the alkaline protease |
-
1996
- 1996-01-26 JP JP8012207A patent/JPH09201195A/en not_active Withdrawn
-
1997
- 1997-01-24 CN CN 97193195 patent/CN1214084A/en active Pending
- 1997-01-24 WO PCT/US1997/001148 patent/WO1997027313A1/en not_active Application Discontinuation
- 1997-01-24 EP EP97903957A patent/EP0876500A4/en not_active Withdrawn
- 1997-01-24 AR ARP970100288A patent/AR005541A1/en unknown
- 1997-01-24 BR BR9707203-6A patent/BR9707203A/en not_active IP Right Cessation
- 1997-01-24 CA CA002243598A patent/CA2243598C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2243598C (en) | 2002-10-22 |
| WO1997027313A1 (en) | 1997-07-31 |
| EP0876500A1 (en) | 1998-11-11 |
| CN1214084A (en) | 1999-04-14 |
| JPH09201195A (en) | 1997-08-05 |
| BR9707203A (en) | 1999-12-28 |
| AR005541A1 (en) | 1999-06-23 |
| EP0876500A4 (en) | 2002-11-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |