CA2241364A1 - Procede permettant de mener un hybridation in situ par fluorescence a parametres multiples et appareil correspondant - Google Patents

Procede permettant de mener un hybridation in situ par fluorescence a parametres multiples et appareil correspondant Download PDF

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Publication number
CA2241364A1
CA2241364A1 CA 2241364 CA2241364A CA2241364A1 CA 2241364 A1 CA2241364 A1 CA 2241364A1 CA 2241364 CA2241364 CA 2241364 CA 2241364 A CA2241364 A CA 2241364A CA 2241364 A1 CA2241364 A1 CA 2241364A1
Authority
CA
Canada
Prior art keywords
interferometer
interferogram
excitation
chromosome
autosomal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2241364
Other languages
English (en)
Inventor
David M. Heffelfinger
Franklin R. Witney
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/640,658 external-priority patent/US5792610A/en
Application filed by Individual filed Critical Individual
Publication of CA2241364A1 publication Critical patent/CA2241364A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention porte sur un procédé permettant d'identifier les chromosomes autosomiques ainsi que les chromosomes sexuels d'un caryotype humain en utilisant un ensemble de sondes d'oligonucléotides marquées de manière combinatoire: (i) ayant chacune une désignation préétablie se distinguant de celle de n'importe quel autre élément de l'ensemble susmentionné et (ii) étant chacune en mesure de s'hybrider de manière spécifique avec un chromosome autosomique ou un chromosome sexuel prédéfinis d'un caryotype humain. Cette invention a trait, de surcroît, à un interféromètre utilisé dans le cadre de ce procédé.
CA 2241364 1995-12-22 1996-12-20 Procede permettant de mener un hybridation in situ par fluorescence a parametres multiples et appareil correspondant Abandoned CA2241364A1 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US57762295A 1995-12-22 1995-12-22
US08/577,622 1995-12-22
US58071795A 1995-12-29 1995-12-29
US08/580,717 1995-12-29
US08/640,658 1996-05-01
US08/640,658 US5792610A (en) 1996-05-01 1996-05-01 Method for conducting multiparametric fluorescence in situ hybridization

Publications (1)

Publication Number Publication Date
CA2241364A1 true CA2241364A1 (fr) 1997-07-03

Family

ID=27416276

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2241364 Abandoned CA2241364A1 (fr) 1995-12-22 1996-12-20 Procede permettant de mener un hybridation in situ par fluorescence a parametres multiples et appareil correspondant

Country Status (4)

Country Link
EP (1) EP0879297A4 (fr)
JP (1) JPH11510707A (fr)
CA (1) CA2241364A1 (fr)
WO (1) WO1997023649A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834203A (en) * 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
GB9807257D0 (en) * 1998-04-03 1998-06-03 Nat Food Research Inst Ministr Apparatus and methods for fourier spectral analysis in a scanning spot microscope
CA2328609A1 (fr) 1998-05-16 1999-11-25 Pe Corporation (Ny) Instrument servant a surveiller l'amplification en chaine par polymerase (pcr) d'adn
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
WO1999062926A1 (fr) * 1998-06-02 1999-12-09 Yale University Hybridation in situ sous fluorescence a parametres multiples
US6174681B1 (en) * 1999-03-05 2001-01-16 Mayo Foundation For Medical Education And Research Method and probe set for detecting cancer
US20050279949A1 (en) 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US7423750B2 (en) 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
EP3312594B1 (fr) 2002-05-17 2019-07-24 Life Technologies Corporation Appareil permettant de différencier des signaux de fluorescence multiples par longueur d'onde d'excitation
US11149299B2 (en) * 2015-06-25 2021-10-19 Ramesh Vallabhaneni Method and system for multiplex profiling of chromosomes in biological samples using target-specific DNA probes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4905169A (en) * 1988-06-02 1990-02-27 The United States Of America As Represented By The United States Department Of Energy Method and apparatus for simultaneously measuring a plurality of spectral wavelengths present in electromagnetic radiation
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US5936731A (en) * 1991-02-22 1999-08-10 Applied Spectral Imaging Ltd. Method for simultaneous detection of multiple fluorophores for in situ hybridization and chromosome painting
FR2784683B1 (fr) * 1998-10-15 2002-12-13 Genset Sa Sondes fluorescentes de peinture chromosomique

Also Published As

Publication number Publication date
JPH11510707A (ja) 1999-09-21
EP0879297A4 (fr) 2003-04-16
WO1997023649A1 (fr) 1997-07-03
EP0879297A1 (fr) 1998-11-25

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Legal Events

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EEER Examination request
FZDE Dead