CA2237336C - Utilisation de la resonance magnetique nucleaire pour designer des ligands qui se lient a des biomolecules cibles - Google Patents

Utilisation de la resonance magnetique nucleaire pour designer des ligands qui se lient a des biomolecules cibles Download PDF

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Publication number
CA2237336C
CA2237336C CA 2237336 CA2237336A CA2237336C CA 2237336 C CA2237336 C CA 2237336C CA 2237336 CA2237336 CA 2237336 CA 2237336 A CA2237336 A CA 2237336A CA 2237336 C CA2237336 C CA 2237336C
Authority
CA
Canada
Prior art keywords
target molecule
ligand
binding
nmr
ligands
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 2237336
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English (en)
Other versions
CA2237336A1 (fr
Inventor
Stephen W. Fesik
Philip J. Hajduk
Edward T. Olejniczak
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AbbVie Inc
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/558,633 external-priority patent/US5891643A/en
Priority claimed from US08/744,701 external-priority patent/US5989827A/en
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Priority to CA2653959A priority Critical patent/CA2653959C/fr
Priority to CA2653517A priority patent/CA2653517C/fr
Priority claimed from PCT/US1996/018312 external-priority patent/WO1997018469A2/fr
Publication of CA2237336A1 publication Critical patent/CA2237336A1/fr
Application granted granted Critical
Publication of CA2237336C publication Critical patent/CA2237336C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

L'invention concerne un procédé pour désigner des composés qui se lient à une molécule cible spécifique. Ce procédé consiste a) à identifier un premier ligand qui se lie à une molécule cible, par spectroscopie RMN à corrélation bidimensionnelle ?15¿N/?1¿H; b) à identifier un second ligand qui se lie à une molécule cible, par spectroscopie RMN à corrélation bidimensionnelle ?15¿N/?1¿H; c) à former un complexe ternaire en liant le premier et le second ligand à la molécule cible; d) à déterminer la structure tridimensionnelle du complexe ternaire et par conséquent l'orientation spatiale du premier et du second ligand sur la molécule cible; et e) à lier le premier et le second ligand pour former le médicament, tout en conservant l'orientation spatiale de l'étape d).
CA 2237336 1995-11-14 1996-11-13 Utilisation de la resonance magnetique nucleaire pour designer des ligands qui se lient a des biomolecules cibles Expired - Lifetime CA2237336C (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA2653959A CA2653959C (fr) 1995-11-14 1996-11-13 Methode d'identification de ligands
CA2653517A CA2653517C (fr) 1995-11-14 1996-11-13 Procede de conception d'un medicament

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US08/558,633 1995-11-14
US08/558,633 US5891643A (en) 1995-11-14 1995-11-14 Use of nuclear magnetic resonance to design ligands to target biomolecules
US67890396A 1996-07-12 1996-07-12
US08/678,903 1996-07-12
US08/744,701 US5989827A (en) 1995-11-14 1996-10-31 Use of nuclear magnetic resonance to design ligands to target biomolecules
US08/744,701 1996-10-31
PCT/US1996/018312 WO1997018469A2 (fr) 1995-11-14 1996-11-13 Utilisation de la resonance magnetique nucleaire pour designer des ligands qui se lient a des biomolecules cibles

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CA2653959A Division CA2653959C (fr) 1995-11-14 1996-11-13 Methode d'identification de ligands
CA2653517A Division CA2653517C (fr) 1995-11-14 1996-11-13 Procede de conception d'un medicament

Publications (2)

Publication Number Publication Date
CA2237336A1 CA2237336A1 (fr) 1997-05-22
CA2237336C true CA2237336C (fr) 2010-08-24

Family

ID=29408017

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2237336 Expired - Lifetime CA2237336C (fr) 1995-11-14 1996-11-13 Utilisation de la resonance magnetique nucleaire pour designer des ligands qui se lient a des biomolecules cibles

Country Status (1)

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CA (1) CA2237336C (fr)

Also Published As

Publication number Publication date
CA2237336A1 (fr) 1997-05-22

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Effective date: 20161114