CA2235415A1 - 2-alkylpyrrolidines - Google Patents
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Abstract
(2R, 3R, 4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and other substituted 2-methylpyrrolidines can be used for the treatment of diabetes.
Description
CA 0223~41~ 199X-04-21 W097/09040 PCT~K96/00373 2--ALRYLPYP~OTTnINES
Field of this invention The present invention relates to the use of 2-alkyl-pyrrolidines in the treatment of diabetes and pharmaceutical 5 compositions contA; n ing these compounds.
Backqround of this invention Diabetes is characterized by an impaired glucose metabolism manifesting itself among other things by an elevated blood glucose level in the diabetic patients. Underlying defects lead to a classification of diabetes into two major groups:
type l diabetes, or insulin demanding diabetes mellitus (IDDM), which arises when patients lack B-cells producing insulin in their pancreatic glands, and type 2 diabetes, or non-insulin dependent diabetes mellitus (NIDDM), which 15 occurs in patients with an impaired B-cell function in association with a range of other abnormalities.
Type l diabetic patients are currently treated with insulin. The majority of type 2 diabetic patients are treated either with sulfonylureas that stimulate B-cell function, with ~-glucosidase inhibitors which decrease carbohydrate uptake from the intestine in association with meals, or with agents that enhance the tissue sensitivity of the patients towards insulin or with insulin. Among the agents applied to ~nh~nce tissue sensitivity towards insu-lin, metformin is a representative example. Examples of ~-glucosidase inhibitors are acarbose and voglibose.
Even though sulfonylureas and ~-glucosidase inhibitors are widely used in the treatment of NIDDM, this therapy is, in most instances, not satisfactory: Thus, in a large number of NIDDM patients, sulfonylureas and ~-glucosidase inhibitors do not suffice to normalize blood sugar levels and the patients are, therefore, at high risk for ac~uiring diabetic complications. Also, many patients gradually lose the ability to respond to treatment with sulfonylureas and are gradually forced into insulin treatment. This shift of patients from oral hypoglycaemic agents to insulin therapy is usually ascribed to exhaustion of the ~-cells in NIDDM patients.
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 In normals as well as in diabetics, the liver produces glucose in order to avoid hypoglycemia. This glucose produc-tion is derived either from the release of glucose from gly-cogen stores or from gluconeogenesis, which is a de novo intracellular synthesis of glucose. In type 2 diabetes, how-ever, the regulation of hepatic glucose output is poorly controlled and is increased, and may be doubled after an overnight fast. Moreover, in these patients there exists a strong correlation between the increased fasting plasma 10 glucose levels and the rate of hepatic glucose production (reviewed in R.A. De Fronzo: Diabetes 37 (1988), 667 - 687;
A. Consoli: Diabetes Care 15 (1992), 430 - 441; and J.E.
Gerich: Horm.Metab.Res. 26 (1992), 18 - 21). Similarly, if type 1 diabetes is not properly controlled by insulin 15 treatment, hepatic glucose production, particularly from glycogen, will be increased and result in fasting hyperglycemia.
Since existing forms of therapy of diabetes does not lead to sufficient glycaemic control and therefore are un-20 satisfying, there is a great demand for novel therapeuticapproaches. Since the liver in diabetes is known to have an increased glucose production, compounds inhibiting this activity are highly desirable.
Recently, patents on inhibitors of the liver specific 25 enzyme, glucose-6-phosphatase, which is necessary for the release of glucose from the liver, have been filed, for example German Offenlequnqsschrift Nos. 4,202,183 and 4,202,184 and Japanese patent application No. 4-58565. All these known compounds are benzene derivatives.
30 International patent application having publication No. WO
92/16640 relates to di-, tri- and tetrasaccharides that are substrates or inhibitors of glycosyltransferase and glyco-sidase enzymes. Some specific compounds mentioned therein CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 are 2,3,4,5-te*rahydroxypiperidine, 3,4,5-trihydroxy-6-methylpiperidine and 3,4-dihydroxy-5-methylpiperidine.
International Patent Application No. WO 92/21657 relates to certain ~-deoxyazapyranoses, e.g. 3,4-dihydroxy-5-methylpiperidine mentioned in Claim 16 thereof. It i5 stated that these compounds have glucosidase inhibiting properties.
European patent application having publication No.
528,495 Al relates to a class of azacyclic compounds, i.e.
compounds comprising an azacyclic ring system substituted by arylmethyloxy or an arylmethylthio moiety. These compunds may be useful as tachykinin antagonists.
European patent application having publication No.
375,651 Al relates to 1,4-dideoxy-1,4-imino-L-allitol and 15 derivatives thereof having glycosidase inhibitory activity.
Moreover, scientifically it is well realized that in-hibition of glycogen phosphorylase is a suitable target for the treatment of diabetes (Martin et al., 1991; Biochemistry 30: 10101-16; Oikonomakos et al., 1994; Eur. J. Drug Metab.
20 Pharmakokin. 3: 185-92). These groups have used glucose ana-logs.
European patent application No. 422,307 relates to prepara-tion of N-glycosyl 1,4-dideoxy-1,4-imino-D-arabinitols as ~-glycosidase inhibitors. These compounds are said to be 25 useful in the treatment of diabaetes mellitus.
European patent application No. 389,723 relates to the preparation of iminoarabinitol derivatives as ~-glucosidase inhibitors.
US patent No. 4,973,602 relates to antiviral (2S,3S,4S) 30 pyrrolidines having benzyloxycarbonyl or an optionally ~ substituted alkylphenyl group in the 1-position. In said US
patent, (2S,3S,4S)-1-([4-chlorophenyl]methyl-2-hydroxy-methyl-3,4-dihydroxypyrrolidine is specifically mentioned.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 European patent application No. 367,747 relates to antiviral (2S,3S,4S) pyrrolidines, e.g. (2S,3S,4S)-2-hydroxymethyl-3,4-dihydroxypyrrolidines having methyl, butyl, hexyl, nonyl, propionyl, 2-hydroxyethyl or 5-hydroxy-5 pentyl in the l-position.
European patent application No. 322.395 describes some pyrrolidines and piperidines, which can be used for the treatment of AIDS. Examples of specific compounds mentioned therein are 2-hydroxymethyl-3,4-dihydroxypyrrolidine and the corresponding 1-methyl derivative.
One object of the present invention is to furnish compounds which can be used as medicaments.
A further object of this invention is to furnish com-pounds which can effectively be used in the treatment of 15 diabetes.
A still further object of this invention is to furnish compounds which can effectively be used as inhibitors of glucose production from the liver.
A further object of this invention is to furnish 20 compounds which can effectively be used as phosphorylase inhibitors.
Brief description of this invention The present invention relates to compounds of the general formula I stated in the claims below.
Surprisingly, it has been found that the compounds stated in the claims, below, have interesting pharmaco-logical properties. For example, the compounds can be used in the treatment of diabetes. Especially, the compounds are active as inhibitors of glucose production from the liver.
30 Consequently, the compounds can be used for the treatment of the increased plasma glucose levels in diabetics.
CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 Detailed descriPtion of this invention Hereinafter, the term alkyl, when used alone or in com-bination with another moiety, is a straight or branched saturated hydrocarbon chaln group which preferably contains not more than 8 carbon atoms, more preferred not more than 4 carbon atoms. Especially preferred alkyl groups are methyl, ethyl, propyl and isopropyl.
The term halogen as used herein refers to chloro, bromo or fluoro, preferably fluoro. Preferably, N-alkylamino is 10 N-methylamino. Preferably, N,N-dialkylamino is N,N-dimethyl-amino. The term acyl as used herein refers to carbonyl sub-stituted with hydrogen, alkyl or phenyl. Herein, cycloalkyl preferably contains 3-7 carbon atoms, more prefered 3-6 carbon atoms. Alkoxy preferably is methoxy or ethoxy.
15 Alkoxycarbonyl preferably is methoxycarbonyl or ethoxy-carbonyl. Aralkyl preferably is benzyl. Trifluoroalkyl preferably is trifluoromethyl or 2,2,2-trifluoroethyl.
Alkene preferably contains not more than 8 carbon atoms and preferably is allyl. The term "one or more" substituents 20 preferably is 1-3 substituents, most preferred 1.
A subgroup of compounds to be used according to this invention are compounds of formula I wherein the two substituets designated by the symbols R3 and R5 are situated at the same side of the plane formed by the 5 membered 25 nitrogen containing ring, and R4 is situated at the opposite side of the plane formed by the 5 membered nitrogen containing ring. Such compounds are either (2S,3S,4S)-2-alkylpyrrolidines or (2R,3R,4R)-2-alkylpyrrolidines. Among these compounds, the (2R,3R,4R)-2-alkylpyrrolidines are 30 preferred.
Examples of compounds to be used according to this invention are compounds of formula I wherein Rl is alkyl which optionally is substituted with one or more of the following CA 0223~41~ Ig9X-04-21 W097/09040 PCT~K96/00373 groups: hydroxy, alkoxy, amino, N-alkylamino, N,N-dialkylamino, alkoxycarbonyl, cycloalkyl or optionally substituted phenyl.
Another example of compounds to be used according to 5 this invention are compounds of ~ormula I wherein Rl is phenylalkyl wherein the phenyl moiety optionally is substituted with one or more of the following groups:
halogen, hydroxy, alkoxy, trifluoromethyl or cyano.
Another subgroup of compounds to be used according to this invention are compounds of formula I wherein R3 and R4 each are hydroxy, and R5 is hydroxymethyl.
The compounds of formula I may be presented as a mixture of isomers which, if desired, may be resolved into the individual pure enantiomers. This resolution may con-15 veniently be performed by fractional crystallization from various solvents, of the salts of compounds of the formula I
with optical active acids or by other methods known Per se, for example, chiral column chromatography. This invention includes all isomers, whether resolved or mixtures thereof.
Examples of pharmaceutically acceptable salts are acid addition salts with non-toxic acids, either inorganic acids such as hydrochloric acid, sulphuric acid and phosphoric acid, or organic acids such as formic acid, acetic acid, propionic acid, succinic acid, gluconic acid, lactic acid, citric acid, ascorbic acid, benzoic acid, embonic acid, methanesulphonic acid and malonic acid.
Preferred compounds to be used according to this invention are 3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, l-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-l-propylpyrrolidine, l-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxYmethyl-l-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 (2,2,2-trifluoroethyl)pyrrolidine, 1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, 3,4-dihydroxy-2-5 hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, 1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and salts and hydrates thereof, preferably (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, (2R,3R,4R)-1-10 cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-propyl-pyrrolidine, (2R,3R,4R)-1-butyl-3,4-dihydroxy-2-hydroxy-methylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, (2R,3R,4R)-1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxy-prop-1-yl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxy-methyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, (2R,3R,4R)-1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-methyl-pyrrolidine, (2S,3S,4S)-1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-25 hydroxymethyl-1-propylpyrrolidine, (2S,3S,4S)-1-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-di-hydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, (2S,3S,4S)-1-benzyl-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2-30 hydroxyethyl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxy-prop-2-yl)pyrrolidine, (2S,3S,4S)-1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and salts and hydrates 35 thereof.
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 Generally, the compounds of formula I are prepared by methods known per se by the skilled art worker, for example as described in the following. The compounds of formula I
can be prepared by joining the C-l and C-4 of xylose 5 together with nitrogen to form the pyrrolidine ring as described in Tetrahedron 42 (1986), 5685 et seq. A variety of functional groups can be introduced in the compounds prepared as outlined above by methods well known to those skilled in the art.
lO More specifically, the compounds of formula I can be prepared as follows:
a) Reacting a compound o~ the general formula II
~ ~ (II) wherein R2, R3, R4, and R5 are as defined in the claims below, with an aldehyde in presence of a reducing agent among which sodium cyanoborohydride is preferred, to form a compound of formula I.
b) Reacting a compound of the general formula II
R5 )~N~R2 (II) CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 wherein R2 R3 R4 and R5 are as defined in the claims below, with a compound of the general formula RlY, wherein Rl is as defined in the claims below, and Y is a leaving group, to form a compound of formula I. The reaction is carried out under alkaline conditions, i.e. in the presence of a base.
The leaving group, Y, may be any suitable leaving group as for example halogen.
c) Reacting a compound of the general formula III
HO ~ N ~ R2 (III) Rl wherein Rl either is as defined in the claims below or is a readily removable protection group, i.e. benzyl, R2 is as defined in the claims below and R3 and R4 are protected hydroxy, i.e. benzyloxy, with a halogenating agent such as 15 thionyl chloride, thionyl bromide, or diethylaminosulfur trifluoride (DAST) and subsequent removal of the protection groups to form a compound of formula I, wherein R1, R3, and R4 are as defined in the claims below, and R5 is methyl substituted with halogen.
20 d) Reacting a compound of the general formula IV
R4 ~R3 X~N~R2 R1 (IV) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 wherein R1 either is as defined in the claims below or is a readily removable protection group, i.e. benzyl, R2 is as defined in the claims below, R3 and R4 are protected hydroxy, i.e. benzyloxy, and X is a leaving group, with a compound of the general formula NHR6R7, wherein the two substituents R6 and R7 may both be alkyl, or one is alkyl and the other is hydrogen or together with NH R6 and R7 form phthalimide, and subsequent removal of the protection groups to form the compounds of formula I, wherein R1, R2' R3, and 10 R4 are as defined in the claims below, and R5 is methyl substituted with amino, N-alkylamino, or N,N-dialkylamino.
The leaving group, X, may be any suitable leaving group as for example halogen.
e) Reacting a compound of the general formula I
Rl .(I) wherein R1 and R2 are as defined in the claims below, and one or two of the groups R3 and R4 is hydroxy and the remaining is protected hydroxy, i.e. benzyl, R5 is as defined in the claims below or is a corresponding protected 20 group, with a halogenating agent such as thionyl chloride, thionyl bromide or diethylaminosulfur trifluoride (DAST) and subsequent removal of the protection groups to form a compound of the formula I, wherein Rl, R2 and R5 are as CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 defined in the claims below, and R3 and R4 are hydroxy or halogen, but not more than one of R3 and R4 is hydroxy.
Pharmaceutical compositions This invention further provides pharmaceutical compositions 5 which comprise at least one compound of formula I or a pharmaceutically acceptable salt thereof in connection with a pharmaceutically acceptable carrier. Such compositions may be in the form of powders, solutions, or suspensions, which may or may not be divided in unit dosage form or in the form of capsules or tablets.
The pharmaceutical compositions of this invention may com-prise carriers, diluents, absorption enhancers, tablet dis-integrating agents and other ingredients which are conven-tionally used in the art. The powders and tablets preferably contain from 5 to 99%, more preferred from 10 to 90 % of the active ingredient. Examples of solid carriers are magnesium carbonate, magnesium stearate, dextrin, lactose, sugar, talc, gelatin, pectin, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, low melting waxes and cocoa butter.
Liquid compositions include sterile solutions, suspen-sions and emulsions suitable for parenteral injection.
The route of administration of the compositions con-taining a compound of formula I may be any route which effectively transports the active compound to its site of 25 action, the oral or nasal route being preferred.
The regimen for any patient to be treated with the com-positions according to the present invention should be de-termined by those skilled in the art. The daily dose to be administered in therapy can be determined by a physician and 30 will depend on the particular compound employed, on the route of administration and on the age and the condition of the patient. A convenient daily dosage can be less than about 1 g, preferably in the range around 10 - 200 mg.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 The present invention is further illustrated by the following examples which, however, are not to be construed as limiting the scope of protection.
The features disclosed in the foregoing description and in the following examples and claims may, both separately and in any combination thereof, be material for realising the invention in diverse forms thereof.
ExamPle 1 (2R,3R,4R)-3,4-Dibenzyloxy-2-(benzyloxymethyl)pyrrolidine (Compound 1) The title compound was prepared by the method described by Overkleeft et al., Tetrahedron 50 (1994), 4215-4224.
ExamPle 2 (2R,3R,4R)-3,4-Dihydroxy-2-(hydroxymethyl)pyrrolidine, 15 hydrochloride (Compound 2) The title compound was prepared by the method described by Overkleeft et al., Tetrahedron 50 (1994), 4215-4224.
Exam~le 3 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-methyl-20 pyrrolidine (Compound 3) A mixture of (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (Compound l) (0.5 g, 1.24 mmol), formic acid (10 ml) and 37 % formaldehyde (7.5 ml) was heated for 3 hours at reflux temperature and evaporated in vacuo. The residue was dissolved in a mixture of ethyl acetate (25 ml) and 1 N
sodium hydroxide (25 ml). The organic phase was isolated, CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 washed once with water, dried over magnesium sulphate and evaporated in vacuo to give (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-methylpyrrolidine (0.49 g, yield: 95%) as a golden oil.
1H-NMR (CDC13) in ppm: ~ 7.2-7.4 (lSH, phenyl), 4.3-4.6 (6H, m, CH2 in benzyl), 3.9 (2H, m, CH20), 3.5-3.7 (2H, m, 2 CH-0-Bn), 3.15 (lH, d, CHCH20), 2.4-2.6 (2H, m, CH2N), 2.4 (3H, s, CH3).
13C-NMR (CDC13) in ppm: ~ 138.4, 138.2, 128.4, 127.9, lo 127.8, 127.6, 127.5, 86.4, 81.6, 73.3, 71.5, 71.0, 70.9, 70.6, 60.4, 41.7.
Example 4 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-methylpyrro-lidine, hydrochloride (Compound 4) 15 A mixture o~ (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-methylpyrrolidine (Compound 3) (38 mg, 0.067 mmol), 10% Pd/C
(30 mg), 4 N HCl (0.1 ml) and 99.9% ethanol (5 ml) was reduced in a Parr apparatus at 40 psi for 20 hours. The mixture was filtered and evaporated in vacuo to give (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-methyl-pyrrolidine, hydrochloride (15 mg, yield 88%) as a yellow oil.
13C-NMR (CD30D) in ppm: ~ 79.1, 78.1, 75.8, 63.4, 60.7, 44.2 Example 5 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-trifluoro-acetylpyrrolidine (Compound 5) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.5 g, 1.5 mmol) was suspended in ethyl 1,1,1-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 trifluoroacetate (20 ml). The reaction mixture was heated at reflux temperature for 16 hours. The mixture was cooled and evaporated in vacuo. The residual oil was purified on a silica gel column with diethylether/hexan (1:1) as eluent 5 giving (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-trifluoroacetylpyrrolidine (0.5 g, yield: 67%) as an oil.
13C-NMR (CDCl3) in ppm: ~ 51.4, 63.4, 66.5, 71.4, 71.6, 73.2, 79.8, 81.3, 116 (q), 127.6, 127.7, 127.9, 128.0, 128.4, 128.6, 137.1, 137.4, 138.1, 156(q).
ExamPle 6 (2R,3R,4R) 3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2,2,2-tri-fluoroethyl)pyrrolidine (Compound 6) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-trifluoro-acetylpyrrolidine (Compound 5) (0.3 g, 0.6 mmol) was 15 dissolved in tetrahydro~uran (20 ml). The mixture was cooled to 0~C and 1 M borane-tetrahydrofuran complex (0.6 ml, 6 mmol) added under nitrogen. The reaction mixture was stirred at 0~C for 2 hours then refluxed for 2 hours. The mixture was cooled and poured into methanol (100 ml). Evaporation n 20 vacuo gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine (0.3 g) as an oil.
1H-NMR (CDC13) in ppm: ~ 2.95 (q,lH); 3.15 (m,2H); 3.5 (d,lH); 3.7 (q,3H); 3.9 (m,lH); 4.2 (m,lH); 4.6 (m,6H); 7.45 (s,15H).
13C-NMR (CDC13) in ppm: ~ 54.9, 55.5, 56.2, 56.8, 58.6, 68.9, 71.2, 71.6, 72.0, 73.4, 81.8, 84.6, 127.7, 127.9, 128.2, 138.1, 138.3.
Example 7 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,2,2-triflu-30 oroethyl)pyrrolidine (compound 7) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-l-(2,2,2-tri-fluoroethyl)pyrrolidine (Compound 6) (0.3 g, 0.6 mmol) was dissolved in 96% ethanol (30 ml), and 10% Pd/C (0.1 g) was added under N2. The compound was reduced in a Parr apparatus (40 Psi) for 16 hours. The reaction mixture was filtered and evaporated in vacuo giving (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine (0.09 g) as an brown oil.
1H-NMR (CDCl3) in ppm: ~ 3.3 (m,lH); 3.4 (m,2H); 3.7 (t,d,2H); 3.8 (t broad, 2H); 3.9-4.1(m,2H); 4.5 (broad s, OH).
ExamPle 8 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-cyclopropyl-methylpyrrolidine, hydrochloride (Compound 8) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.25 g, 0.62 mmol) was dissolved in methyl-isobutylketone (15 ml). Potassium carbonate (0.17 g, 1.2 mmol) and potassium iodide (0.03 g, 0.18 mol) was added.
After stirring for 10 min at 25~C cyclopropylmethylbromide (0.078 ml, 0.81 mmol) was added. The mixture was stirred under a N2 atmosphere at 80~C for 24 hours and evaporated in vacuo. Water (20 ml) was added and extraction with methylene chloride (3 x 20 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded a yellow oil. Purification of the crude product twice on a silica gel column (l: Eluent: CH2Cl2/MeOH (19:1) - and 2: Eluent: CH2Cl2/MeOH (39:1)) gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-cyclopropylmethylpyrrolidine (0.213 g, yield: 75%) as an oil.
lH-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 4.5 (m, 6H);
3.8 (broad s, 2H); 3.70-3.45 (m, 2H); 3.38 (s) and 3.32 (s) (alltogether lH); 2.86 (dd, lH); 2.74 (m, lH); 2.63 (dd, CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 lH); 2.11 (dd, lH); 1.0-0.8 (m,lH); 0.46 (t, 2H); 0.10 (d, 2H).
Conversion of the ~ree base into the hydrochloride salt using 2M HCl(g) in diethylether gave (2R,3R,4R)-2-5 benzyloxymethyl-3,4-dibenzyloxy-1-cyclopropylmethyl-pyrrolidine, hydrochloride (0.14 g, yield 46~), melting point: 66-67~C.
Example 9 (2R,3R,4R)-l-Cyclopropylmethyl-3,4-dihydroxy-2-hydroxyme-10 thylpyrrolidine, hydrochloride (Compound 9).
To a solution of (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxy-methyl-1-cyclopropylmethylpyrrolidine, hydrochloride (Compound 8) (0.14 g, 0.28 mmol) in 96~ ethanol (15 ml) was added 10~ Pd/C (50 mg) and 1 M hydrochloric acid (0.1 ml).
15 The reaction mixture was hydrogenated in Parr apparatus at 40 psi for 24 hours. The mixture was filtered and concentrated in vacuo giving (2R,3R,4R)-1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, hydrochloride as a yellow oil (0.063 g, yield: 100~).
lH-NMR (CD30D) in ppm: ~ 3.96 (broad s, 2H); 3.90 (s,lHj; 3.71-3.39 (m, 4H); 3.00 (dd, lH); 1.24-1.08 (m, lH);
0.75 (d, 2H); 0.43 (t, 2H).
ExamPle 10 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-butylpyrro-lidine (Compound 10).
(2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.7 g, 1.7 mmol) was dissolved in dry methanol. Butyric aldehyde (0.153 ml, 1.7 mmol) and sodium cyanoborohydride (0.107 g, 1.7 mmol) was added. A solution CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 of anhydrous hydrogen chloride in diethylether (2 M) was added dropwise until pH 6. The resulting mixture was stirred for 24 hours at room temperature under a nitrogen atmosphere and evaporated in vacuo. Addition of lM sodium hydroxide (50 5 ml), extraction of the product with diethylether (2 x 50 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo gave the title compound as a crude oil (0.644 g). Puri~ication of the crude product on a silica gel column (Eluent: methylene chloride/
10 methanol (l9:1)) afforded (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-butylpyrrolidine (0.243 g, yield: 30~) as an oil.
1H-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 4.51 (m, 4H);
Field of this invention The present invention relates to the use of 2-alkyl-pyrrolidines in the treatment of diabetes and pharmaceutical 5 compositions contA; n ing these compounds.
Backqround of this invention Diabetes is characterized by an impaired glucose metabolism manifesting itself among other things by an elevated blood glucose level in the diabetic patients. Underlying defects lead to a classification of diabetes into two major groups:
type l diabetes, or insulin demanding diabetes mellitus (IDDM), which arises when patients lack B-cells producing insulin in their pancreatic glands, and type 2 diabetes, or non-insulin dependent diabetes mellitus (NIDDM), which 15 occurs in patients with an impaired B-cell function in association with a range of other abnormalities.
Type l diabetic patients are currently treated with insulin. The majority of type 2 diabetic patients are treated either with sulfonylureas that stimulate B-cell function, with ~-glucosidase inhibitors which decrease carbohydrate uptake from the intestine in association with meals, or with agents that enhance the tissue sensitivity of the patients towards insulin or with insulin. Among the agents applied to ~nh~nce tissue sensitivity towards insu-lin, metformin is a representative example. Examples of ~-glucosidase inhibitors are acarbose and voglibose.
Even though sulfonylureas and ~-glucosidase inhibitors are widely used in the treatment of NIDDM, this therapy is, in most instances, not satisfactory: Thus, in a large number of NIDDM patients, sulfonylureas and ~-glucosidase inhibitors do not suffice to normalize blood sugar levels and the patients are, therefore, at high risk for ac~uiring diabetic complications. Also, many patients gradually lose the ability to respond to treatment with sulfonylureas and are gradually forced into insulin treatment. This shift of patients from oral hypoglycaemic agents to insulin therapy is usually ascribed to exhaustion of the ~-cells in NIDDM patients.
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 In normals as well as in diabetics, the liver produces glucose in order to avoid hypoglycemia. This glucose produc-tion is derived either from the release of glucose from gly-cogen stores or from gluconeogenesis, which is a de novo intracellular synthesis of glucose. In type 2 diabetes, how-ever, the regulation of hepatic glucose output is poorly controlled and is increased, and may be doubled after an overnight fast. Moreover, in these patients there exists a strong correlation between the increased fasting plasma 10 glucose levels and the rate of hepatic glucose production (reviewed in R.A. De Fronzo: Diabetes 37 (1988), 667 - 687;
A. Consoli: Diabetes Care 15 (1992), 430 - 441; and J.E.
Gerich: Horm.Metab.Res. 26 (1992), 18 - 21). Similarly, if type 1 diabetes is not properly controlled by insulin 15 treatment, hepatic glucose production, particularly from glycogen, will be increased and result in fasting hyperglycemia.
Since existing forms of therapy of diabetes does not lead to sufficient glycaemic control and therefore are un-20 satisfying, there is a great demand for novel therapeuticapproaches. Since the liver in diabetes is known to have an increased glucose production, compounds inhibiting this activity are highly desirable.
Recently, patents on inhibitors of the liver specific 25 enzyme, glucose-6-phosphatase, which is necessary for the release of glucose from the liver, have been filed, for example German Offenlequnqsschrift Nos. 4,202,183 and 4,202,184 and Japanese patent application No. 4-58565. All these known compounds are benzene derivatives.
30 International patent application having publication No. WO
92/16640 relates to di-, tri- and tetrasaccharides that are substrates or inhibitors of glycosyltransferase and glyco-sidase enzymes. Some specific compounds mentioned therein CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 are 2,3,4,5-te*rahydroxypiperidine, 3,4,5-trihydroxy-6-methylpiperidine and 3,4-dihydroxy-5-methylpiperidine.
International Patent Application No. WO 92/21657 relates to certain ~-deoxyazapyranoses, e.g. 3,4-dihydroxy-5-methylpiperidine mentioned in Claim 16 thereof. It i5 stated that these compounds have glucosidase inhibiting properties.
European patent application having publication No.
528,495 Al relates to a class of azacyclic compounds, i.e.
compounds comprising an azacyclic ring system substituted by arylmethyloxy or an arylmethylthio moiety. These compunds may be useful as tachykinin antagonists.
European patent application having publication No.
375,651 Al relates to 1,4-dideoxy-1,4-imino-L-allitol and 15 derivatives thereof having glycosidase inhibitory activity.
Moreover, scientifically it is well realized that in-hibition of glycogen phosphorylase is a suitable target for the treatment of diabetes (Martin et al., 1991; Biochemistry 30: 10101-16; Oikonomakos et al., 1994; Eur. J. Drug Metab.
20 Pharmakokin. 3: 185-92). These groups have used glucose ana-logs.
European patent application No. 422,307 relates to prepara-tion of N-glycosyl 1,4-dideoxy-1,4-imino-D-arabinitols as ~-glycosidase inhibitors. These compounds are said to be 25 useful in the treatment of diabaetes mellitus.
European patent application No. 389,723 relates to the preparation of iminoarabinitol derivatives as ~-glucosidase inhibitors.
US patent No. 4,973,602 relates to antiviral (2S,3S,4S) 30 pyrrolidines having benzyloxycarbonyl or an optionally ~ substituted alkylphenyl group in the 1-position. In said US
patent, (2S,3S,4S)-1-([4-chlorophenyl]methyl-2-hydroxy-methyl-3,4-dihydroxypyrrolidine is specifically mentioned.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 European patent application No. 367,747 relates to antiviral (2S,3S,4S) pyrrolidines, e.g. (2S,3S,4S)-2-hydroxymethyl-3,4-dihydroxypyrrolidines having methyl, butyl, hexyl, nonyl, propionyl, 2-hydroxyethyl or 5-hydroxy-5 pentyl in the l-position.
European patent application No. 322.395 describes some pyrrolidines and piperidines, which can be used for the treatment of AIDS. Examples of specific compounds mentioned therein are 2-hydroxymethyl-3,4-dihydroxypyrrolidine and the corresponding 1-methyl derivative.
One object of the present invention is to furnish compounds which can be used as medicaments.
A further object of this invention is to furnish com-pounds which can effectively be used in the treatment of 15 diabetes.
A still further object of this invention is to furnish compounds which can effectively be used as inhibitors of glucose production from the liver.
A further object of this invention is to furnish 20 compounds which can effectively be used as phosphorylase inhibitors.
Brief description of this invention The present invention relates to compounds of the general formula I stated in the claims below.
Surprisingly, it has been found that the compounds stated in the claims, below, have interesting pharmaco-logical properties. For example, the compounds can be used in the treatment of diabetes. Especially, the compounds are active as inhibitors of glucose production from the liver.
30 Consequently, the compounds can be used for the treatment of the increased plasma glucose levels in diabetics.
CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 Detailed descriPtion of this invention Hereinafter, the term alkyl, when used alone or in com-bination with another moiety, is a straight or branched saturated hydrocarbon chaln group which preferably contains not more than 8 carbon atoms, more preferred not more than 4 carbon atoms. Especially preferred alkyl groups are methyl, ethyl, propyl and isopropyl.
The term halogen as used herein refers to chloro, bromo or fluoro, preferably fluoro. Preferably, N-alkylamino is 10 N-methylamino. Preferably, N,N-dialkylamino is N,N-dimethyl-amino. The term acyl as used herein refers to carbonyl sub-stituted with hydrogen, alkyl or phenyl. Herein, cycloalkyl preferably contains 3-7 carbon atoms, more prefered 3-6 carbon atoms. Alkoxy preferably is methoxy or ethoxy.
15 Alkoxycarbonyl preferably is methoxycarbonyl or ethoxy-carbonyl. Aralkyl preferably is benzyl. Trifluoroalkyl preferably is trifluoromethyl or 2,2,2-trifluoroethyl.
Alkene preferably contains not more than 8 carbon atoms and preferably is allyl. The term "one or more" substituents 20 preferably is 1-3 substituents, most preferred 1.
A subgroup of compounds to be used according to this invention are compounds of formula I wherein the two substituets designated by the symbols R3 and R5 are situated at the same side of the plane formed by the 5 membered 25 nitrogen containing ring, and R4 is situated at the opposite side of the plane formed by the 5 membered nitrogen containing ring. Such compounds are either (2S,3S,4S)-2-alkylpyrrolidines or (2R,3R,4R)-2-alkylpyrrolidines. Among these compounds, the (2R,3R,4R)-2-alkylpyrrolidines are 30 preferred.
Examples of compounds to be used according to this invention are compounds of formula I wherein Rl is alkyl which optionally is substituted with one or more of the following CA 0223~41~ Ig9X-04-21 W097/09040 PCT~K96/00373 groups: hydroxy, alkoxy, amino, N-alkylamino, N,N-dialkylamino, alkoxycarbonyl, cycloalkyl or optionally substituted phenyl.
Another example of compounds to be used according to 5 this invention are compounds of ~ormula I wherein Rl is phenylalkyl wherein the phenyl moiety optionally is substituted with one or more of the following groups:
halogen, hydroxy, alkoxy, trifluoromethyl or cyano.
Another subgroup of compounds to be used according to this invention are compounds of formula I wherein R3 and R4 each are hydroxy, and R5 is hydroxymethyl.
The compounds of formula I may be presented as a mixture of isomers which, if desired, may be resolved into the individual pure enantiomers. This resolution may con-15 veniently be performed by fractional crystallization from various solvents, of the salts of compounds of the formula I
with optical active acids or by other methods known Per se, for example, chiral column chromatography. This invention includes all isomers, whether resolved or mixtures thereof.
Examples of pharmaceutically acceptable salts are acid addition salts with non-toxic acids, either inorganic acids such as hydrochloric acid, sulphuric acid and phosphoric acid, or organic acids such as formic acid, acetic acid, propionic acid, succinic acid, gluconic acid, lactic acid, citric acid, ascorbic acid, benzoic acid, embonic acid, methanesulphonic acid and malonic acid.
Preferred compounds to be used according to this invention are 3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, l-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-l-propylpyrrolidine, l-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxYmethyl-l-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 (2,2,2-trifluoroethyl)pyrrolidine, 1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, 3,4-dihydroxy-2-5 hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, 1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and salts and hydrates thereof, preferably (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, (2R,3R,4R)-1-10 cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-propyl-pyrrolidine, (2R,3R,4R)-1-butyl-3,4-dihydroxy-2-hydroxy-methylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, (2R,3R,4R)-1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxy-prop-1-yl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxy-methyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, (2R,3R,4R)-1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-methyl-pyrrolidine, (2S,3S,4S)-1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-25 hydroxymethyl-1-propylpyrrolidine, (2S,3S,4S)-1-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-di-hydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, (2S,3S,4S)-1-benzyl-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2-30 hydroxyethyl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxy-prop-2-yl)pyrrolidine, (2S,3S,4S)-1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and salts and hydrates 35 thereof.
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 Generally, the compounds of formula I are prepared by methods known per se by the skilled art worker, for example as described in the following. The compounds of formula I
can be prepared by joining the C-l and C-4 of xylose 5 together with nitrogen to form the pyrrolidine ring as described in Tetrahedron 42 (1986), 5685 et seq. A variety of functional groups can be introduced in the compounds prepared as outlined above by methods well known to those skilled in the art.
lO More specifically, the compounds of formula I can be prepared as follows:
a) Reacting a compound o~ the general formula II
~ ~ (II) wherein R2, R3, R4, and R5 are as defined in the claims below, with an aldehyde in presence of a reducing agent among which sodium cyanoborohydride is preferred, to form a compound of formula I.
b) Reacting a compound of the general formula II
R5 )~N~R2 (II) CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 wherein R2 R3 R4 and R5 are as defined in the claims below, with a compound of the general formula RlY, wherein Rl is as defined in the claims below, and Y is a leaving group, to form a compound of formula I. The reaction is carried out under alkaline conditions, i.e. in the presence of a base.
The leaving group, Y, may be any suitable leaving group as for example halogen.
c) Reacting a compound of the general formula III
HO ~ N ~ R2 (III) Rl wherein Rl either is as defined in the claims below or is a readily removable protection group, i.e. benzyl, R2 is as defined in the claims below and R3 and R4 are protected hydroxy, i.e. benzyloxy, with a halogenating agent such as 15 thionyl chloride, thionyl bromide, or diethylaminosulfur trifluoride (DAST) and subsequent removal of the protection groups to form a compound of formula I, wherein R1, R3, and R4 are as defined in the claims below, and R5 is methyl substituted with halogen.
20 d) Reacting a compound of the general formula IV
R4 ~R3 X~N~R2 R1 (IV) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 wherein R1 either is as defined in the claims below or is a readily removable protection group, i.e. benzyl, R2 is as defined in the claims below, R3 and R4 are protected hydroxy, i.e. benzyloxy, and X is a leaving group, with a compound of the general formula NHR6R7, wherein the two substituents R6 and R7 may both be alkyl, or one is alkyl and the other is hydrogen or together with NH R6 and R7 form phthalimide, and subsequent removal of the protection groups to form the compounds of formula I, wherein R1, R2' R3, and 10 R4 are as defined in the claims below, and R5 is methyl substituted with amino, N-alkylamino, or N,N-dialkylamino.
The leaving group, X, may be any suitable leaving group as for example halogen.
e) Reacting a compound of the general formula I
Rl .(I) wherein R1 and R2 are as defined in the claims below, and one or two of the groups R3 and R4 is hydroxy and the remaining is protected hydroxy, i.e. benzyl, R5 is as defined in the claims below or is a corresponding protected 20 group, with a halogenating agent such as thionyl chloride, thionyl bromide or diethylaminosulfur trifluoride (DAST) and subsequent removal of the protection groups to form a compound of the formula I, wherein Rl, R2 and R5 are as CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 defined in the claims below, and R3 and R4 are hydroxy or halogen, but not more than one of R3 and R4 is hydroxy.
Pharmaceutical compositions This invention further provides pharmaceutical compositions 5 which comprise at least one compound of formula I or a pharmaceutically acceptable salt thereof in connection with a pharmaceutically acceptable carrier. Such compositions may be in the form of powders, solutions, or suspensions, which may or may not be divided in unit dosage form or in the form of capsules or tablets.
The pharmaceutical compositions of this invention may com-prise carriers, diluents, absorption enhancers, tablet dis-integrating agents and other ingredients which are conven-tionally used in the art. The powders and tablets preferably contain from 5 to 99%, more preferred from 10 to 90 % of the active ingredient. Examples of solid carriers are magnesium carbonate, magnesium stearate, dextrin, lactose, sugar, talc, gelatin, pectin, tragacanth, methyl cellulose, sodium carboxymethyl cellulose, low melting waxes and cocoa butter.
Liquid compositions include sterile solutions, suspen-sions and emulsions suitable for parenteral injection.
The route of administration of the compositions con-taining a compound of formula I may be any route which effectively transports the active compound to its site of 25 action, the oral or nasal route being preferred.
The regimen for any patient to be treated with the com-positions according to the present invention should be de-termined by those skilled in the art. The daily dose to be administered in therapy can be determined by a physician and 30 will depend on the particular compound employed, on the route of administration and on the age and the condition of the patient. A convenient daily dosage can be less than about 1 g, preferably in the range around 10 - 200 mg.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 The present invention is further illustrated by the following examples which, however, are not to be construed as limiting the scope of protection.
The features disclosed in the foregoing description and in the following examples and claims may, both separately and in any combination thereof, be material for realising the invention in diverse forms thereof.
ExamPle 1 (2R,3R,4R)-3,4-Dibenzyloxy-2-(benzyloxymethyl)pyrrolidine (Compound 1) The title compound was prepared by the method described by Overkleeft et al., Tetrahedron 50 (1994), 4215-4224.
ExamPle 2 (2R,3R,4R)-3,4-Dihydroxy-2-(hydroxymethyl)pyrrolidine, 15 hydrochloride (Compound 2) The title compound was prepared by the method described by Overkleeft et al., Tetrahedron 50 (1994), 4215-4224.
Exam~le 3 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-methyl-20 pyrrolidine (Compound 3) A mixture of (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (Compound l) (0.5 g, 1.24 mmol), formic acid (10 ml) and 37 % formaldehyde (7.5 ml) was heated for 3 hours at reflux temperature and evaporated in vacuo. The residue was dissolved in a mixture of ethyl acetate (25 ml) and 1 N
sodium hydroxide (25 ml). The organic phase was isolated, CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 washed once with water, dried over magnesium sulphate and evaporated in vacuo to give (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-methylpyrrolidine (0.49 g, yield: 95%) as a golden oil.
1H-NMR (CDC13) in ppm: ~ 7.2-7.4 (lSH, phenyl), 4.3-4.6 (6H, m, CH2 in benzyl), 3.9 (2H, m, CH20), 3.5-3.7 (2H, m, 2 CH-0-Bn), 3.15 (lH, d, CHCH20), 2.4-2.6 (2H, m, CH2N), 2.4 (3H, s, CH3).
13C-NMR (CDC13) in ppm: ~ 138.4, 138.2, 128.4, 127.9, lo 127.8, 127.6, 127.5, 86.4, 81.6, 73.3, 71.5, 71.0, 70.9, 70.6, 60.4, 41.7.
Example 4 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-methylpyrro-lidine, hydrochloride (Compound 4) 15 A mixture o~ (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-methylpyrrolidine (Compound 3) (38 mg, 0.067 mmol), 10% Pd/C
(30 mg), 4 N HCl (0.1 ml) and 99.9% ethanol (5 ml) was reduced in a Parr apparatus at 40 psi for 20 hours. The mixture was filtered and evaporated in vacuo to give (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-methyl-pyrrolidine, hydrochloride (15 mg, yield 88%) as a yellow oil.
13C-NMR (CD30D) in ppm: ~ 79.1, 78.1, 75.8, 63.4, 60.7, 44.2 Example 5 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-trifluoro-acetylpyrrolidine (Compound 5) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.5 g, 1.5 mmol) was suspended in ethyl 1,1,1-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 trifluoroacetate (20 ml). The reaction mixture was heated at reflux temperature for 16 hours. The mixture was cooled and evaporated in vacuo. The residual oil was purified on a silica gel column with diethylether/hexan (1:1) as eluent 5 giving (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-trifluoroacetylpyrrolidine (0.5 g, yield: 67%) as an oil.
13C-NMR (CDCl3) in ppm: ~ 51.4, 63.4, 66.5, 71.4, 71.6, 73.2, 79.8, 81.3, 116 (q), 127.6, 127.7, 127.9, 128.0, 128.4, 128.6, 137.1, 137.4, 138.1, 156(q).
ExamPle 6 (2R,3R,4R) 3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2,2,2-tri-fluoroethyl)pyrrolidine (Compound 6) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-trifluoro-acetylpyrrolidine (Compound 5) (0.3 g, 0.6 mmol) was 15 dissolved in tetrahydro~uran (20 ml). The mixture was cooled to 0~C and 1 M borane-tetrahydrofuran complex (0.6 ml, 6 mmol) added under nitrogen. The reaction mixture was stirred at 0~C for 2 hours then refluxed for 2 hours. The mixture was cooled and poured into methanol (100 ml). Evaporation n 20 vacuo gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine (0.3 g) as an oil.
1H-NMR (CDC13) in ppm: ~ 2.95 (q,lH); 3.15 (m,2H); 3.5 (d,lH); 3.7 (q,3H); 3.9 (m,lH); 4.2 (m,lH); 4.6 (m,6H); 7.45 (s,15H).
13C-NMR (CDC13) in ppm: ~ 54.9, 55.5, 56.2, 56.8, 58.6, 68.9, 71.2, 71.6, 72.0, 73.4, 81.8, 84.6, 127.7, 127.9, 128.2, 138.1, 138.3.
Example 7 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,2,2-triflu-30 oroethyl)pyrrolidine (compound 7) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-l-(2,2,2-tri-fluoroethyl)pyrrolidine (Compound 6) (0.3 g, 0.6 mmol) was dissolved in 96% ethanol (30 ml), and 10% Pd/C (0.1 g) was added under N2. The compound was reduced in a Parr apparatus (40 Psi) for 16 hours. The reaction mixture was filtered and evaporated in vacuo giving (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine (0.09 g) as an brown oil.
1H-NMR (CDCl3) in ppm: ~ 3.3 (m,lH); 3.4 (m,2H); 3.7 (t,d,2H); 3.8 (t broad, 2H); 3.9-4.1(m,2H); 4.5 (broad s, OH).
ExamPle 8 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-cyclopropyl-methylpyrrolidine, hydrochloride (Compound 8) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.25 g, 0.62 mmol) was dissolved in methyl-isobutylketone (15 ml). Potassium carbonate (0.17 g, 1.2 mmol) and potassium iodide (0.03 g, 0.18 mol) was added.
After stirring for 10 min at 25~C cyclopropylmethylbromide (0.078 ml, 0.81 mmol) was added. The mixture was stirred under a N2 atmosphere at 80~C for 24 hours and evaporated in vacuo. Water (20 ml) was added and extraction with methylene chloride (3 x 20 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded a yellow oil. Purification of the crude product twice on a silica gel column (l: Eluent: CH2Cl2/MeOH (19:1) - and 2: Eluent: CH2Cl2/MeOH (39:1)) gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-cyclopropylmethylpyrrolidine (0.213 g, yield: 75%) as an oil.
lH-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 4.5 (m, 6H);
3.8 (broad s, 2H); 3.70-3.45 (m, 2H); 3.38 (s) and 3.32 (s) (alltogether lH); 2.86 (dd, lH); 2.74 (m, lH); 2.63 (dd, CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 lH); 2.11 (dd, lH); 1.0-0.8 (m,lH); 0.46 (t, 2H); 0.10 (d, 2H).
Conversion of the ~ree base into the hydrochloride salt using 2M HCl(g) in diethylether gave (2R,3R,4R)-2-5 benzyloxymethyl-3,4-dibenzyloxy-1-cyclopropylmethyl-pyrrolidine, hydrochloride (0.14 g, yield 46~), melting point: 66-67~C.
Example 9 (2R,3R,4R)-l-Cyclopropylmethyl-3,4-dihydroxy-2-hydroxyme-10 thylpyrrolidine, hydrochloride (Compound 9).
To a solution of (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxy-methyl-1-cyclopropylmethylpyrrolidine, hydrochloride (Compound 8) (0.14 g, 0.28 mmol) in 96~ ethanol (15 ml) was added 10~ Pd/C (50 mg) and 1 M hydrochloric acid (0.1 ml).
15 The reaction mixture was hydrogenated in Parr apparatus at 40 psi for 24 hours. The mixture was filtered and concentrated in vacuo giving (2R,3R,4R)-1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, hydrochloride as a yellow oil (0.063 g, yield: 100~).
lH-NMR (CD30D) in ppm: ~ 3.96 (broad s, 2H); 3.90 (s,lHj; 3.71-3.39 (m, 4H); 3.00 (dd, lH); 1.24-1.08 (m, lH);
0.75 (d, 2H); 0.43 (t, 2H).
ExamPle 10 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-butylpyrro-lidine (Compound 10).
(2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.7 g, 1.7 mmol) was dissolved in dry methanol. Butyric aldehyde (0.153 ml, 1.7 mmol) and sodium cyanoborohydride (0.107 g, 1.7 mmol) was added. A solution CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 of anhydrous hydrogen chloride in diethylether (2 M) was added dropwise until pH 6. The resulting mixture was stirred for 24 hours at room temperature under a nitrogen atmosphere and evaporated in vacuo. Addition of lM sodium hydroxide (50 5 ml), extraction of the product with diethylether (2 x 50 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo gave the title compound as a crude oil (0.644 g). Puri~ication of the crude product on a silica gel column (Eluent: methylene chloride/
10 methanol (l9:1)) afforded (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-l-butylpyrrolidine (0.243 g, yield: 30~) as an oil.
1H-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 4.51 (m, 4H);
4.45 (2 s, 2H); 3.90 (m, 2H); 3.S7 (m, 2H); 3.23 (s) and 3.18 (s) (alltogether lH); 2.94-2.77 (m, lH); 2.72 (dd, lH);
2.55 (dd, lH); 2.40-2.24 (m, lH); 1.56-1.18 (m, 4H); 0.90 (t, 3H).
ExamPle 11 (2R,3R,4R)-l-Butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 20 hydrochloride (Compound 11) The title compound was synthesized as described for compound 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-butylpyrrolidine (Compound 10) (0.243 g, 0.53 mmol), ethanol (30 ml), 10~ Pd/C (0.07 g) and excess of lM hydro-chloric acid to convert the amine to the hydrochloride salt.
(2R,3R,4R)-1-Butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, hydrochloride was obtained as a yellow syrup (0.098 g, yield 82%).
H-NMR (CD30D) in ppm: ~ 4.15 (s, lH); 3.88 (m, 3H);
3.54 (m, lH); 3.4 (m, 3H); 3.1 (m, lH); 1.71 (m, 2H); 1.39 (m, 2H); 0.95 (t, 3H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 12 (2R,3R,4R)-1-Acetyl-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 12) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.642 g, 1.6 mmol) was dissolved in dry methylene chloride (15 ml) under a nitrogen atmosphere.
Triethylamine (0.288 ml, 2.1 mmol) and acetyl chloride (0.125 ml, 1.8 mmol) were added, and the mixture was stirred for 2 hours at room temperature. Water (20 ml) was added, 10 the layers were separated and the water phase was extracted twice with methylene chloride (2 x 20 ml).
Drying of the combined organic phases with magnesium sulphate and evaporation of the solvent in vacuo gave the title compound as an crude oil (0.7 g, yield 99%).
15 Purification on silica gel (Eluent: methylene chloride/
methanol (19:1)) afforded (2R,3R,4R)-l-acetyl-3,4-dibenzyloxy-2-benzyloxymethylpyrrolidine (0.595 g, yield 83%) as an oil.
lH-NMR (CDC13) in ppm: ~ 7.28 (m, 15H); 4.65-4.33 (m, 7H); 4.12-3.46 (m, 6H); 2.06 (s) and 2.00 (s) (alltogether 3H).
Example 13 (2R,3R,4R)-1-Acetyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 13) 25 The title compound was synthesized as described for compound 9 using (2R,3R,4R)-l-acetyl-3,4-dibenZylOxy-2-benzyloxy-methylpyrrolidine (Compound 12) (0.595 g, 1.3 mmol), ethanol (30 ml), methanol (10 ml), 10% Pd/C (0.10 g) and a catalytic amount of 1 M hydrochloric acid. Purification of the product on silica gel (Eluent: Ethyl acetate/methanol (1:1)) afford-CA 0223~41~ 1998-04-21 WO 97/09040 PCT/DK96~ ,7 ed (2R,3R~4R)-l-acetyl-3~4-dihydroxy-2-hydroxymeth pyrrolidine (0.2 g, yield: 86%) as an oil.
1H-NMR (CD30D) in ppm: ~ 4.10 (broad s, 2H); 3.95-3.70 (m, 4H); 3.51-3.38 (m, lH); 2.20 (s) and 2.10 (s) (alltogether 3H).
Example 14 (2R,3R,4R)-l-Allyl-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (Compound 14) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (1.025 g, 2.5 mmol) was dissolved in methylisobutylketone (15 ml). Triethylamine (0.53 ml, 3.8 mmol) and potassium iodide (0.04 g) were added. The mixture was stirred under a nitrogen atmosphere for 4 hours at 80~C
and 24 hours at room temperature and evaporated in vacuo.
15 Water (40 ml) was added and extraction with methylene chloride (3 x 40 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded a yellow oil. Purification of the crude product on a silica gel column (Eluent: Heptane/ethyl acetate (9:1)) 20 gave (2R,3R,4R)-l-allyl-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (0.91 g, yield: 81%) as an oil.
1H-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 6.02-5.82 (m, lH); 5.25-5.05 (m, 2H); 4.50 (m, 4H); 4.45 (s) and 4.43 (s) (alltogether 2H); 3.89 (m, 2H); 3.67-3.48 (m, 3H); 3.20 (s) 25 and 3.13 (s) (alltogether lH); 3.02 (dd, lH); 2.78 (dd, lH);
2.60 (dd, lH).
-Example 15 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-propylpyrrolidine (Compound 15) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 The title compound was synthesized as described for compound 9 using (2R,3R,4R)-1-allyl-3,4-dibenzyloxy-2-benzyloxyme-thylpyrrolidine (compound 14) (0.910 g, 2.1 mmol), ethanol (100 ml), 10% Pd/C (0.2 g) and excess of 1 M hydrochloric acid to convert the amine to the hydrochloride salt. After evaporation of the solvent in vacuo the compound was purified on silica gel (Eluent: 2-propanol/25% ammonium hydroxide (4:1)) and (2R,3R,4R)-3,4-dihydroxy-2-hydrox-ymethyl-1-propylpyrrolidine was obtained as a yellow 10 crystals (0.279 g, yield: 78%). Melting point: 79-80~C.
1H-NMR (CD30D) in ppm: ~ 3.92 (m, 2H); 3.67 (m, 2H);
3.03 (s) and 2.98 (s) (alltogether lH); 2.87-2.59 (m, 2H);
2.46-2.20 (m, 2H); 1.50 (m, 2H); 0.90 (t, 3H).
ExamPle 16 (2R,3R,4R)-1-Benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 16) (2R,3R,4R)-2-Hydroxy-3,4-dihydroxymethylpyrrolidine hydro-chloride (compound 2) (0.265 g, 1.6 mmol) was dissolved in dry methanol (25 ml), and benzaldehyde (0.159 ml, 1.6 mmol) 20 and sodium cyanoborohydride (0.098 g, 1.6 mmol) was added. A
solution of anhydrous hydrogen chloride in diethylether (2M) was added dropwise until pH 6. The resulting mixture was stirred for 24 hours at room temperature under a nitrogen atmosphere and evaporated in vacuo. Purification of the 25 product on silica gel (Eluent: 2-propanol/methanol (4:1)) afforded (2R,3R,4R)-1-benzyl-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine (0.304 g, yield: 87%) as an oil.
lH-NMR (CD30D) in ppm: ~ 7.40-7.20 (m, 5H); 4.10 (d, J
= 14Hz, lH); 3.92 (m, 2H); 3.70 (m, 2H); 3.50 (d, J = 14Hz, lH); 2.90-2.60 (m, 3H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 17 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihy-droxyprop-1-yl)pyrrolidine (Compound 17) The title compound was synthesized as described for compound 10 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 1) (0.5 g, 1.2 mmol), methanol (30 ml), glyceraldehyde (0.134 g, 1.5 mmol) and sodium cyanoboro-hydride (0.094 g, 1.5 mmol). Purification of the crude product on silica gel (Eluent: Ethyl acetate) afforded (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine (0.424 g, yield: 72%) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.28 (m, 15H); 4.48 (s, 4H);
4.43 (m, 2H); 3.95 (m, lH); 3.84 (m, lH); 3.78-3.25 (m, 8H);
3.23 (s) and 3.18 (s) (alltogether lH); 3.02-2.39 (m, 4H).
ExamPle 18 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,3-dihy-droxyprop-l-yl)pyrrolidine, hydrochloride (Compound 18) The title compound was synthesized as described for compound 20 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine (Compound 17) (0.424 g, 0.89 mmol), ethanol (80 ml), 10% Pd/c (0.1 g), and excess of 4 M
hydrochloric acid to convert the amine to the hydrochloride salt. (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,3-- 25 dihydroxyprop-1-yl)pyrrolidine hydrochloride was obtained as white crystals (0.216 g, yield: 100%) with melting point above 230~C (decomposition).
1H-NMR (CD30D) in ppm: ~ 4.22 (broad s, lH); 4.0 (m, 4H); 3.8-3.2 (m, 7H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 19 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-phthalimi-doethyl)pyrrolidine (Compound 19) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.73 g, 1.8 mmol), N-(2-bromoethyl)phthalimide (0.686 g, 2.7 mmol), triethylamine (0.5 ml, 3.6 mmol) and a catalytic amount of potassium iodide was dissolved in dry dimethyl~ormamide (30 ml). The mixture was stirred ~or 24h at 70~C, cooled to room temperature and evaporated in vacuo.
10 Water (60 ml) was added and extraction with methylene chloride (3 x60 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded an oil. Purification twice on silica gel (Eluent 1:
Heptane/ethyl acetate (1:1) and eluent 2: Petroleum ether/diethylether (2:1)) gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2-phthalimidoethyl)pyrrolidine (0.64 g, yield: 61%) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.72 (m, 2H); 7.60 (m, 2H);
7.24 (m, 15H); 4.50 (m, 4H); 4.40 (m, 2H); 4.00-3.64 (m, 20 4H); 3.53-3.23 (m, 4H); 2.79 (dd, lH); 2.28 (dd, lH); 2.7-2.5 (m, lH).
Example 20 (2R,3R,4R)-1-(2-Aminoethyl)-3,4-dibenzyloxy-2-benzyloxyme-thylpyrrolidine (Compound 20) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-phthal-imidoethyl)pyrrolidine (Compound 19) (0.64 g, 1.1 mmol) was dissolved in ethanol (20 ml) and hydrazin, hydrate (0.215 ml, 4.4 mmol) was added. The clear solution was stirred for 4 hours at 40~C and for 18 hours at room temperature. The 30 white precipitate was filtered off and the filtrate CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 evaporated in vacuo. The residue was partitioned between aqueous hydrochloric acid and methylene chloride. The water phase adjusted to pH 11 with 2N sodium hydroxide and extracted with methylene chloride (2 x 100 ml) and with 5 diethylether (100 ml). Drying of the combined organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded the crude product as an oil. Purification on silica gel (Eluent: Ethyl acetate) gave (2R,3R,4R)-1-(2-aminoethyl)-3,4-dibenzyloxy-2-benzyloxymethylpyrrolidine (0.015 g) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.22 (m, 15H); 4.50 (m, 4H);
4.40 (m, 2H); 3.95 (m, lH); 3.83 (m, lH); 3.65-3.30 (m, 4H);
3.29 (s) and 3.24 (s) (alltogether lH); 3.13-2.98 (m, lH);
2.82 (dd, lH); 2.67 (dd, lH); 2.6 (m, lH).
ExamPle 21 (2R,3R,4R)-1-(2-Aminoethyl)-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine (Compound 21) The title compound can be synthesized as described for compound 9 using (2R,3R,4R)-1-(2-aminoethyl)-3,4-20 dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 20) as starting material.
Example 22 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxy-ethyl)pyrrolidine (Compound 22) 25 The title compound was prepared as described for compound 8 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 1) (0.51 g, 1.27 mmol) and 2-chloroethanol (0.1 ml, 1.49 mmol) as starting material. (2R,3R,4R)-3,4-CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 Dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxyethyl)pyrrolidine was obtained as a golden oil (0.48 g, yield: 85 %).
1H-NMR (CDC13) in ppm: S 7.3 (m, 15H); 4.5 (m, 6H); 4.0 (broad d, lH); 3.9 (broad d, lH); 3.6 (m, 4H); 3.25 (d,lH);
3.05 (m, lH); 2.9 (m, lH); 2.68 (dd, lH); 2.6 (dt, lH).
ExamPle 23 (2R,3R,4R)-3,4-Dihydroxy-1-(2-hydroxyethyl)-2-hydroxymethyl-pyrrolidine, hydrochloride (Compound 23) The title compound was synthesized as described for compound 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxyethyl)pyrrolidine (Compound 22) as starting mate-rial. (2R,3R,4R)-3,4-Dihydroxy-1-(2-hydroxyethyl)-2-hydroxymethylpyrrolidine, hydrochloride was obtained as an golden oil (0.23 g, yield 100%).
lH-NMR (CD30D) in ppm: S 4.2 (broad s, lH), 3.85-4.05 (m, 5H); 3.5-3.8 (m, 4H); 3.25-3.4 (m, lH) ExamPle 24 Experimental ~rotocol and results For in vivo studies, female ob/OB mice (20 g) fasted for 3 20 hours were used. Test compounds or NaCl (0.9~; controls) were a~minictered intraveneously (hereinafter designated i.v.). Glucagon were administered subcutaneously (herein-after designated s.c.) in order to increase hepatic glucose output derived from glycogen. Blood samples were drawn from 25 the orbital vain and analyzed for glucose using a glucose oxidase method.
Rat hepatocytes were isolated using a standard two step collagenase technique, and cultured onto collagen coated CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 culture dishes for 72 hours in medium 199 with the addition of dexamethazone (0.1 ~M); penicillin/Streptomycin ((100 u/100 ~g)/ml) and insulin (1 nM). During the last 24 hours, the hepatocytes were cultured in the presence of high levels 5 of insulin (5 nM) and glucose (15 mM), which result in the incorporation of glucose into glycogen. Therefore, at the time of the experiment, the cells mimic livers from fed ani-mals.
Experiments were initiated after 48 hours of culture by 2 times wash of cells and addition of a 20 mM HEPES experi-mental buffer including balanced salts, but without glucose.
The test compound was added simultaneously with the ex-perimental buffer. To some cultures, glucagon (0.5 nM) was added after 10 minutes in order to stimulate glucose produc-15 tion from liver cells. The glucose released into the m~; ~,reflecting the glucose production of the liver cells, was measured 70 minutes after the start of the experiment and standardized to cellular DNA content.
Phosphorylase was either purchased from Sigma or extracted from rat livers according to Stalmans et. al. (Eur.J.Bio-chem. 49 (1974), 415). The activity of phosphorylase was determined as described by Bergmeyer (1983; in: Meth. of Enzymatic Analysis, 2, 293-295, Weinheim, (ed.) Verlag Chemie).
25 The activity of the glycogen debranching enzyme, ~-1,6-glucosidase, was determined as described by Brown and Brown (1966; in : Meth. in Enzymology, 8, 515-524, Neufeld and - Ginsburg (Eds.) Academic Press).
Table 1 below demonstrate the efficacy of (2R,3R,4R)-3,4-30 dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) in lowering the glucagon mediated increase in plasma glucose.
The effects are compared to those in control animals and CA 022354l5 l998-04-2l W097/09040 PCT~K96/00373 those in animals treated with 6 fold higher doses of the model ~-1,6-glucosidase inhibitor l-deoxynojirimycin (hereinafter designated dNOJ).
Table 1. Effect~ of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and the model ~-1,6-glucosidase inhibitor on the glucagon e~;Atea increase in blood glucose in mice. Numbers are aVerage9 t 5.DN-5.
¦Delta plasma glucose ¦ (mmoles/L) Control animals 6.3 + 1.0 lO Compound 2 0.5 + 0.6 (8 mg/kg) dNOJ (SO mg/kg) 5.7 + 1.2 Table 1 demonstrates that (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) represents a potent 15 principle for reducing blood glucose. In contrast, the ~-1,6-glucosidase inhibitor, dNOJ, was unable to reduce blood glucose.
Table 2 below shows the results obtained with (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) on 20 basal and glucagon stimulated glycogenolysis. The e~fects are compared to those exerted by the a-1,6-glucosidase inhibitor: dNOJ.
-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 Table 2. Effect~ of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (compound 2) on baseline and glucagon stimulated glucose production from cultured - liver cells. Values are expressed relative to the basal glucose production. Results obtained with the model ~-1,6 glucosidase inhibitor, 1-deoxynojirimycin, are shown for ~ ison.
Glucose production Without With glucagon glucagon (O.5nM) No addition: 100 % 233%
Compound 2 (l~M): 19 % 41%
lO dNOJ (50~M): 92 % 195~
The results clearly demonstrate the ability of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) to inhibit basal and glucagon stimulated hepatocyte glucose production, while inhibition of ~-1,6-glucosidase (dNOJ) was insufficient to suppress hepatic glucose production.
Table 3 compares the potency of various 2-methylpyrrolidines with the potency of dNOJ on various cellular and enzymatic activities.
CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 Table 3. Effects of selected pyrrolidines c~ _~-ed to effects of dNOJ on different enzymatic activities. ~he results are the concentrations of the compound~
resulting in half maximal activity (I.C.50 ~moles/l).
5 Compound Phospho- 1,6-glucosidaqe rylase dNOJ >200 1.1 2 0.7 0.18a 23 10 n.d.
7 145 n.d.
10 9 169 n.d.
11 60 n.d.
n.d.: not determined a) From: Fleet et al. (Tetrahedron 20 (1986), 5685) It is apparent from the presented data in table 3 that the 2-methylpyrrolidines of this invention are potent inhibitors of liver cell glucose production. Moreover, it is also demonstrated that phosphorylase is inhibited by these compounds in similar low concentrations.
20 Table 3 also demonstrates that the potent model inhibitor of liver ~-1,6-glucosidase was unable to inhibit either liver cell glucose production or phosphorylase.
While ~-1,6-glucosidase inhibition is recognized as a prin-ciple of reducing blood glucose in association with a 25 carbohydrate rich meal, the finding that compounds of this invention are able to reduce blood glucose arising from hepatic glucose production, i.e. blood glucose in the CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 fasting state, is new and surprising. The surprising aspect is substantiated by the presented negative results with the - model ~-1,6-glucosidase inhibitor: dNOJ. These results are in agreement with the results presented by Bollen and Stal-5 mans (Eur.J.Biochem. 181 (1980), 775), who also concluded that ~-1,6-glucosidase inhibition is an insufficient principle for inhibition of liver cell glucose production.
Furthermore, it was clearly demonstrated by Sels et al.
(Netherland J.Med. 44 (1994), 198) that fasting plasma 10 glucose of type 2 diabetic patients was not reduced after treatment with the ~-1,6-glucosidase inhibitor, miglitol.
In conclusion, the data demonstrates that the compounds of this invention are able to reduce blood glucose and inhibits glucose production from liver cells. It is also demonstrated 15 that the reduction in blood glucose and liver cell glucose production by the compounds of formula I according to this invention is mediated by inhibition of phosphorylase.
Consequently, the compounds of formula I can be used to inhibit both the baseline and glucagon stimulated glucose 20 production from liver cells. Therefore, compounds of formula I will be usefull in the treatment of diabetes.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 Example 25 Tablets Tablets which are suitable for oral administration and which contain the below-mentioned components are produced in a 5 manner known Per se granulating the active and the auxiliary substances and making them into tablets.
A typical tablet contains 50 mg of the compound of formula I, 100 mg of lactose, 30 mg of corn starch, 3 mg of talc powder, 3 mg of colloidal silicon dioxide and 2 mg of lO magnesium stearate.
Exam~le 26 Capsules Capsules which are suitable for oral administration contain the below-mentioned components are produced in a manner 15 known Per se mixing the active substances with the auxiliary substances and putting them into gelatine capsules.
- A typical capsule contains 50 mg of the compound of formula I, 100 mg of lactose, 30 mg of corn starch, 3 mg of talc -CA 022354l5 l998-04-2l W097/09040 PCT~K96/00373 powder, 3 mg of colloidal silicon dioxide and 2 mg of magnesium stearate.
2.55 (dd, lH); 2.40-2.24 (m, lH); 1.56-1.18 (m, 4H); 0.90 (t, 3H).
ExamPle 11 (2R,3R,4R)-l-Butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 20 hydrochloride (Compound 11) The title compound was synthesized as described for compound 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-butylpyrrolidine (Compound 10) (0.243 g, 0.53 mmol), ethanol (30 ml), 10~ Pd/C (0.07 g) and excess of lM hydro-chloric acid to convert the amine to the hydrochloride salt.
(2R,3R,4R)-1-Butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, hydrochloride was obtained as a yellow syrup (0.098 g, yield 82%).
H-NMR (CD30D) in ppm: ~ 4.15 (s, lH); 3.88 (m, 3H);
3.54 (m, lH); 3.4 (m, 3H); 3.1 (m, lH); 1.71 (m, 2H); 1.39 (m, 2H); 0.95 (t, 3H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 12 (2R,3R,4R)-1-Acetyl-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 12) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.642 g, 1.6 mmol) was dissolved in dry methylene chloride (15 ml) under a nitrogen atmosphere.
Triethylamine (0.288 ml, 2.1 mmol) and acetyl chloride (0.125 ml, 1.8 mmol) were added, and the mixture was stirred for 2 hours at room temperature. Water (20 ml) was added, 10 the layers were separated and the water phase was extracted twice with methylene chloride (2 x 20 ml).
Drying of the combined organic phases with magnesium sulphate and evaporation of the solvent in vacuo gave the title compound as an crude oil (0.7 g, yield 99%).
15 Purification on silica gel (Eluent: methylene chloride/
methanol (19:1)) afforded (2R,3R,4R)-l-acetyl-3,4-dibenzyloxy-2-benzyloxymethylpyrrolidine (0.595 g, yield 83%) as an oil.
lH-NMR (CDC13) in ppm: ~ 7.28 (m, 15H); 4.65-4.33 (m, 7H); 4.12-3.46 (m, 6H); 2.06 (s) and 2.00 (s) (alltogether 3H).
Example 13 (2R,3R,4R)-1-Acetyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 13) 25 The title compound was synthesized as described for compound 9 using (2R,3R,4R)-l-acetyl-3,4-dibenZylOxy-2-benzyloxy-methylpyrrolidine (Compound 12) (0.595 g, 1.3 mmol), ethanol (30 ml), methanol (10 ml), 10% Pd/C (0.10 g) and a catalytic amount of 1 M hydrochloric acid. Purification of the product on silica gel (Eluent: Ethyl acetate/methanol (1:1)) afford-CA 0223~41~ 1998-04-21 WO 97/09040 PCT/DK96~ ,7 ed (2R,3R~4R)-l-acetyl-3~4-dihydroxy-2-hydroxymeth pyrrolidine (0.2 g, yield: 86%) as an oil.
1H-NMR (CD30D) in ppm: ~ 4.10 (broad s, 2H); 3.95-3.70 (m, 4H); 3.51-3.38 (m, lH); 2.20 (s) and 2.10 (s) (alltogether 3H).
Example 14 (2R,3R,4R)-l-Allyl-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (Compound 14) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (1.025 g, 2.5 mmol) was dissolved in methylisobutylketone (15 ml). Triethylamine (0.53 ml, 3.8 mmol) and potassium iodide (0.04 g) were added. The mixture was stirred under a nitrogen atmosphere for 4 hours at 80~C
and 24 hours at room temperature and evaporated in vacuo.
15 Water (40 ml) was added and extraction with methylene chloride (3 x 40 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded a yellow oil. Purification of the crude product on a silica gel column (Eluent: Heptane/ethyl acetate (9:1)) 20 gave (2R,3R,4R)-l-allyl-3,4-dibenzyloxy-2-benzyloxymethyl-pyrrolidine (0.91 g, yield: 81%) as an oil.
1H-NMR (CDCl3) in ppm: ~ 7.28 (m, 15H); 6.02-5.82 (m, lH); 5.25-5.05 (m, 2H); 4.50 (m, 4H); 4.45 (s) and 4.43 (s) (alltogether 2H); 3.89 (m, 2H); 3.67-3.48 (m, 3H); 3.20 (s) 25 and 3.13 (s) (alltogether lH); 3.02 (dd, lH); 2.78 (dd, lH);
2.60 (dd, lH).
-Example 15 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-propylpyrrolidine (Compound 15) CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 The title compound was synthesized as described for compound 9 using (2R,3R,4R)-1-allyl-3,4-dibenzyloxy-2-benzyloxyme-thylpyrrolidine (compound 14) (0.910 g, 2.1 mmol), ethanol (100 ml), 10% Pd/C (0.2 g) and excess of 1 M hydrochloric acid to convert the amine to the hydrochloride salt. After evaporation of the solvent in vacuo the compound was purified on silica gel (Eluent: 2-propanol/25% ammonium hydroxide (4:1)) and (2R,3R,4R)-3,4-dihydroxy-2-hydrox-ymethyl-1-propylpyrrolidine was obtained as a yellow 10 crystals (0.279 g, yield: 78%). Melting point: 79-80~C.
1H-NMR (CD30D) in ppm: ~ 3.92 (m, 2H); 3.67 (m, 2H);
3.03 (s) and 2.98 (s) (alltogether lH); 2.87-2.59 (m, 2H);
2.46-2.20 (m, 2H); 1.50 (m, 2H); 0.90 (t, 3H).
ExamPle 16 (2R,3R,4R)-1-Benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 16) (2R,3R,4R)-2-Hydroxy-3,4-dihydroxymethylpyrrolidine hydro-chloride (compound 2) (0.265 g, 1.6 mmol) was dissolved in dry methanol (25 ml), and benzaldehyde (0.159 ml, 1.6 mmol) 20 and sodium cyanoborohydride (0.098 g, 1.6 mmol) was added. A
solution of anhydrous hydrogen chloride in diethylether (2M) was added dropwise until pH 6. The resulting mixture was stirred for 24 hours at room temperature under a nitrogen atmosphere and evaporated in vacuo. Purification of the 25 product on silica gel (Eluent: 2-propanol/methanol (4:1)) afforded (2R,3R,4R)-1-benzyl-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine (0.304 g, yield: 87%) as an oil.
lH-NMR (CD30D) in ppm: ~ 7.40-7.20 (m, 5H); 4.10 (d, J
= 14Hz, lH); 3.92 (m, 2H); 3.70 (m, 2H); 3.50 (d, J = 14Hz, lH); 2.90-2.60 (m, 3H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 17 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihy-droxyprop-1-yl)pyrrolidine (Compound 17) The title compound was synthesized as described for compound 10 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 1) (0.5 g, 1.2 mmol), methanol (30 ml), glyceraldehyde (0.134 g, 1.5 mmol) and sodium cyanoboro-hydride (0.094 g, 1.5 mmol). Purification of the crude product on silica gel (Eluent: Ethyl acetate) afforded (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine (0.424 g, yield: 72%) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.28 (m, 15H); 4.48 (s, 4H);
4.43 (m, 2H); 3.95 (m, lH); 3.84 (m, lH); 3.78-3.25 (m, 8H);
3.23 (s) and 3.18 (s) (alltogether lH); 3.02-2.39 (m, 4H).
ExamPle 18 (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,3-dihy-droxyprop-l-yl)pyrrolidine, hydrochloride (Compound 18) The title compound was synthesized as described for compound 20 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine (Compound 17) (0.424 g, 0.89 mmol), ethanol (80 ml), 10% Pd/c (0.1 g), and excess of 4 M
hydrochloric acid to convert the amine to the hydrochloride salt. (2R,3R,4R)-3,4-Dihydroxy-2-hydroxymethyl-1-(2,3-- 25 dihydroxyprop-1-yl)pyrrolidine hydrochloride was obtained as white crystals (0.216 g, yield: 100%) with melting point above 230~C (decomposition).
1H-NMR (CD30D) in ppm: ~ 4.22 (broad s, lH); 4.0 (m, 4H); 3.8-3.2 (m, 7H).
CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 ExamPle 19 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-phthalimi-doethyl)pyrrolidine (Compound 19) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 1) (0.73 g, 1.8 mmol), N-(2-bromoethyl)phthalimide (0.686 g, 2.7 mmol), triethylamine (0.5 ml, 3.6 mmol) and a catalytic amount of potassium iodide was dissolved in dry dimethyl~ormamide (30 ml). The mixture was stirred ~or 24h at 70~C, cooled to room temperature and evaporated in vacuo.
10 Water (60 ml) was added and extraction with methylene chloride (3 x60 ml), drying of the organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded an oil. Purification twice on silica gel (Eluent 1:
Heptane/ethyl acetate (1:1) and eluent 2: Petroleum ether/diethylether (2:1)) gave (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2-phthalimidoethyl)pyrrolidine (0.64 g, yield: 61%) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.72 (m, 2H); 7.60 (m, 2H);
7.24 (m, 15H); 4.50 (m, 4H); 4.40 (m, 2H); 4.00-3.64 (m, 20 4H); 3.53-3.23 (m, 4H); 2.79 (dd, lH); 2.28 (dd, lH); 2.7-2.5 (m, lH).
Example 20 (2R,3R,4R)-1-(2-Aminoethyl)-3,4-dibenzyloxy-2-benzyloxyme-thylpyrrolidine (Compound 20) (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-phthal-imidoethyl)pyrrolidine (Compound 19) (0.64 g, 1.1 mmol) was dissolved in ethanol (20 ml) and hydrazin, hydrate (0.215 ml, 4.4 mmol) was added. The clear solution was stirred for 4 hours at 40~C and for 18 hours at room temperature. The 30 white precipitate was filtered off and the filtrate CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 evaporated in vacuo. The residue was partitioned between aqueous hydrochloric acid and methylene chloride. The water phase adjusted to pH 11 with 2N sodium hydroxide and extracted with methylene chloride (2 x 100 ml) and with 5 diethylether (100 ml). Drying of the combined organic phases with magnesium sulphate and evaporation of the solvent in vacuo afforded the crude product as an oil. Purification on silica gel (Eluent: Ethyl acetate) gave (2R,3R,4R)-1-(2-aminoethyl)-3,4-dibenzyloxy-2-benzyloxymethylpyrrolidine (0.015 g) as an oil.
1H-NMR (CDC13) in ppm: ~ 7.22 (m, 15H); 4.50 (m, 4H);
4.40 (m, 2H); 3.95 (m, lH); 3.83 (m, lH); 3.65-3.30 (m, 4H);
3.29 (s) and 3.24 (s) (alltogether lH); 3.13-2.98 (m, lH);
2.82 (dd, lH); 2.67 (dd, lH); 2.6 (m, lH).
ExamPle 21 (2R,3R,4R)-1-(2-Aminoethyl)-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine (Compound 21) The title compound can be synthesized as described for compound 9 using (2R,3R,4R)-1-(2-aminoethyl)-3,4-20 dibenzyloxy-2-benzyloxymethylpyrrolidine (Compound 20) as starting material.
Example 22 (2R,3R,4R)-3,4-Dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxy-ethyl)pyrrolidine (Compound 22) 25 The title compound was prepared as described for compound 8 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethylpyrro-lidine (Compound 1) (0.51 g, 1.27 mmol) and 2-chloroethanol (0.1 ml, 1.49 mmol) as starting material. (2R,3R,4R)-3,4-CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 Dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxyethyl)pyrrolidine was obtained as a golden oil (0.48 g, yield: 85 %).
1H-NMR (CDC13) in ppm: S 7.3 (m, 15H); 4.5 (m, 6H); 4.0 (broad d, lH); 3.9 (broad d, lH); 3.6 (m, 4H); 3.25 (d,lH);
3.05 (m, lH); 2.9 (m, lH); 2.68 (dd, lH); 2.6 (dt, lH).
ExamPle 23 (2R,3R,4R)-3,4-Dihydroxy-1-(2-hydroxyethyl)-2-hydroxymethyl-pyrrolidine, hydrochloride (Compound 23) The title compound was synthesized as described for compound 9 using (2R,3R,4R)-3,4-dibenzyloxy-2-benzyloxymethyl-1-(2-hydroxyethyl)pyrrolidine (Compound 22) as starting mate-rial. (2R,3R,4R)-3,4-Dihydroxy-1-(2-hydroxyethyl)-2-hydroxymethylpyrrolidine, hydrochloride was obtained as an golden oil (0.23 g, yield 100%).
lH-NMR (CD30D) in ppm: S 4.2 (broad s, lH), 3.85-4.05 (m, 5H); 3.5-3.8 (m, 4H); 3.25-3.4 (m, lH) ExamPle 24 Experimental ~rotocol and results For in vivo studies, female ob/OB mice (20 g) fasted for 3 20 hours were used. Test compounds or NaCl (0.9~; controls) were a~minictered intraveneously (hereinafter designated i.v.). Glucagon were administered subcutaneously (herein-after designated s.c.) in order to increase hepatic glucose output derived from glycogen. Blood samples were drawn from 25 the orbital vain and analyzed for glucose using a glucose oxidase method.
Rat hepatocytes were isolated using a standard two step collagenase technique, and cultured onto collagen coated CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 culture dishes for 72 hours in medium 199 with the addition of dexamethazone (0.1 ~M); penicillin/Streptomycin ((100 u/100 ~g)/ml) and insulin (1 nM). During the last 24 hours, the hepatocytes were cultured in the presence of high levels 5 of insulin (5 nM) and glucose (15 mM), which result in the incorporation of glucose into glycogen. Therefore, at the time of the experiment, the cells mimic livers from fed ani-mals.
Experiments were initiated after 48 hours of culture by 2 times wash of cells and addition of a 20 mM HEPES experi-mental buffer including balanced salts, but without glucose.
The test compound was added simultaneously with the ex-perimental buffer. To some cultures, glucagon (0.5 nM) was added after 10 minutes in order to stimulate glucose produc-15 tion from liver cells. The glucose released into the m~; ~,reflecting the glucose production of the liver cells, was measured 70 minutes after the start of the experiment and standardized to cellular DNA content.
Phosphorylase was either purchased from Sigma or extracted from rat livers according to Stalmans et. al. (Eur.J.Bio-chem. 49 (1974), 415). The activity of phosphorylase was determined as described by Bergmeyer (1983; in: Meth. of Enzymatic Analysis, 2, 293-295, Weinheim, (ed.) Verlag Chemie).
25 The activity of the glycogen debranching enzyme, ~-1,6-glucosidase, was determined as described by Brown and Brown (1966; in : Meth. in Enzymology, 8, 515-524, Neufeld and - Ginsburg (Eds.) Academic Press).
Table 1 below demonstrate the efficacy of (2R,3R,4R)-3,4-30 dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) in lowering the glucagon mediated increase in plasma glucose.
The effects are compared to those in control animals and CA 022354l5 l998-04-2l W097/09040 PCT~K96/00373 those in animals treated with 6 fold higher doses of the model ~-1,6-glucosidase inhibitor l-deoxynojirimycin (hereinafter designated dNOJ).
Table 1. Effect~ of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine and the model ~-1,6-glucosidase inhibitor on the glucagon e~;Atea increase in blood glucose in mice. Numbers are aVerage9 t 5.DN-5.
¦Delta plasma glucose ¦ (mmoles/L) Control animals 6.3 + 1.0 lO Compound 2 0.5 + 0.6 (8 mg/kg) dNOJ (SO mg/kg) 5.7 + 1.2 Table 1 demonstrates that (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) represents a potent 15 principle for reducing blood glucose. In contrast, the ~-1,6-glucosidase inhibitor, dNOJ, was unable to reduce blood glucose.
Table 2 below shows the results obtained with (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) on 20 basal and glucagon stimulated glycogenolysis. The e~fects are compared to those exerted by the a-1,6-glucosidase inhibitor: dNOJ.
-CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 Table 2. Effect~ of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (compound 2) on baseline and glucagon stimulated glucose production from cultured - liver cells. Values are expressed relative to the basal glucose production. Results obtained with the model ~-1,6 glucosidase inhibitor, 1-deoxynojirimycin, are shown for ~ ison.
Glucose production Without With glucagon glucagon (O.5nM) No addition: 100 % 233%
Compound 2 (l~M): 19 % 41%
lO dNOJ (50~M): 92 % 195~
The results clearly demonstrate the ability of (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethylpyrrolidine (Compound 2) to inhibit basal and glucagon stimulated hepatocyte glucose production, while inhibition of ~-1,6-glucosidase (dNOJ) was insufficient to suppress hepatic glucose production.
Table 3 compares the potency of various 2-methylpyrrolidines with the potency of dNOJ on various cellular and enzymatic activities.
CA 0223~4l~ 1998-04-2l W097/09040 PCT~K96/00373 Table 3. Effects of selected pyrrolidines c~ _~-ed to effects of dNOJ on different enzymatic activities. ~he results are the concentrations of the compound~
resulting in half maximal activity (I.C.50 ~moles/l).
5 Compound Phospho- 1,6-glucosidaqe rylase dNOJ >200 1.1 2 0.7 0.18a 23 10 n.d.
7 145 n.d.
10 9 169 n.d.
11 60 n.d.
n.d.: not determined a) From: Fleet et al. (Tetrahedron 20 (1986), 5685) It is apparent from the presented data in table 3 that the 2-methylpyrrolidines of this invention are potent inhibitors of liver cell glucose production. Moreover, it is also demonstrated that phosphorylase is inhibited by these compounds in similar low concentrations.
20 Table 3 also demonstrates that the potent model inhibitor of liver ~-1,6-glucosidase was unable to inhibit either liver cell glucose production or phosphorylase.
While ~-1,6-glucosidase inhibition is recognized as a prin-ciple of reducing blood glucose in association with a 25 carbohydrate rich meal, the finding that compounds of this invention are able to reduce blood glucose arising from hepatic glucose production, i.e. blood glucose in the CA 0223~41~ 1998-04-21 W097/09040 PCT~K96/00373 fasting state, is new and surprising. The surprising aspect is substantiated by the presented negative results with the - model ~-1,6-glucosidase inhibitor: dNOJ. These results are in agreement with the results presented by Bollen and Stal-5 mans (Eur.J.Biochem. 181 (1980), 775), who also concluded that ~-1,6-glucosidase inhibition is an insufficient principle for inhibition of liver cell glucose production.
Furthermore, it was clearly demonstrated by Sels et al.
(Netherland J.Med. 44 (1994), 198) that fasting plasma 10 glucose of type 2 diabetic patients was not reduced after treatment with the ~-1,6-glucosidase inhibitor, miglitol.
In conclusion, the data demonstrates that the compounds of this invention are able to reduce blood glucose and inhibits glucose production from liver cells. It is also demonstrated 15 that the reduction in blood glucose and liver cell glucose production by the compounds of formula I according to this invention is mediated by inhibition of phosphorylase.
Consequently, the compounds of formula I can be used to inhibit both the baseline and glucagon stimulated glucose 20 production from liver cells. Therefore, compounds of formula I will be usefull in the treatment of diabetes.
CA 0223~4l~ l998-04-2l W097/09040 PCT~K96/00373 Example 25 Tablets Tablets which are suitable for oral administration and which contain the below-mentioned components are produced in a 5 manner known Per se granulating the active and the auxiliary substances and making them into tablets.
A typical tablet contains 50 mg of the compound of formula I, 100 mg of lactose, 30 mg of corn starch, 3 mg of talc powder, 3 mg of colloidal silicon dioxide and 2 mg of lO magnesium stearate.
Exam~le 26 Capsules Capsules which are suitable for oral administration contain the below-mentioned components are produced in a manner 15 known Per se mixing the active substances with the auxiliary substances and putting them into gelatine capsules.
- A typical capsule contains 50 mg of the compound of formula I, 100 mg of lactose, 30 mg of corn starch, 3 mg of talc -CA 022354l5 l998-04-2l W097/09040 PCT~K96/00373 powder, 3 mg of colloidal silicon dioxide and 2 mg of magnesium stearate.
Claims (31)
1. The use of compounds of the general formula I
wherein R1 is hydrogen, acyl, alkene, cycloalkyl or alkyl which optionally is substituted with one or more of the following groups: hydroxy, alkoxy, amino, N-alkylamino, N,N-dialkylamino, halogen, cycloalkyl, optionally substituted phenyl or alkoxycarbonyl, R2 is hydrogen or alkyl, R3 and R4, which are the same or different, independent of each other, is hydrogen, halogen, hydroxy, mercapto or amino which is optionally substituted with alkyl or aralkyl, and R5 is alkyl substituted with hydroxy, halogen, amino, N-alkylamino, N,N-dialkylamino or mercapto, or salts or hydrates thereof as a liver glycogen phosphorylase inhibitory agent.
wherein R1 is hydrogen, acyl, alkene, cycloalkyl or alkyl which optionally is substituted with one or more of the following groups: hydroxy, alkoxy, amino, N-alkylamino, N,N-dialkylamino, halogen, cycloalkyl, optionally substituted phenyl or alkoxycarbonyl, R2 is hydrogen or alkyl, R3 and R4, which are the same or different, independent of each other, is hydrogen, halogen, hydroxy, mercapto or amino which is optionally substituted with alkyl or aralkyl, and R5 is alkyl substituted with hydroxy, halogen, amino, N-alkylamino, N,N-dialkylamino or mercapto, or salts or hydrates thereof as a liver glycogen phosphorylase inhibitory agent.
2. The use, according to claim 1, wherein the compound of formula I contains at least 2 hydroxy groups.
3. The use, according to anyone of the previous claims, wherein the compound of formula I contains at least 3 hydroxy groups.
4. The use, according to anyone of the previous claims, wherein in the compound of formula I the two substituents designated by the symbols R3 and R5 are situated at the same side of the plane formed by the 5 membered nitrogen containing ring, and R4 is situated at the opposite side of the plane formed by the 5 membered nitogen containing ring.
5. The use, according to anyone of the previous claims, wherein in the compound of formula I R1 represents hydrogen, acyl or alkyl which is optionally substituted with one or more of the following groups: hydroxy, alkoxy, amino, N-alkylamino, N,N-dialkylamino, phenyl or alkoxycarbonyl.
6. The use, according to the previous claim, wherein in the compound of formula I R1 is hydrogen or alkyl, preferably methyl.
7. The use, according to the previous claim, wherein in the compound of formula I R1 is hydrogen.
8. The use, according to anyone of the previous claims, wherein in the compound of formula I the optionally substituted phenyl group is phenyl substituted with one or more of the following groups: halogen, hydroxy, alkoxy, trifluoroalkyl or cyano.
9. The use, according to anyone of the previous claims, wherein in the compound of formula I R2 is hydrogen or alkyl, preferably methyl.
10. The use, according to anyone of the previous claims, wherein in the compound of formula I R2 is hydrogen.
11. The use, according to anyone of the previous claims, wherein in the compound of formula I R3 is hydrogen, hydroxy, halogen or amino.
12. The use, according to anyone of the previous claim, wherein in the compound of formula I R3 is hydroxy, halogen or amino.
13. Compounds, according to the previous claim, wherein R3 is hydroxy or halogen, preferably fluoro.
14. The use, according to the previous claim, wherein R3 is hydroxy.
15. The use, according to anyone of the previous claims, wherein in the compound of formula I R4 is hydrogen, hydroxy, halogen or amino.
16. The use, according to anyone of the previous claims, wherein in the compound of formula I R4 is hydroxy, halogen or amino.
17. The use, according to anyone of the previous claims, wherein in the compound of formula I R4 is hydrogen or halogen, preferably fluoro.
18. The use, according to anyone of the previous claims, wherein in the compound of formula I R4 is hydroxy or halogen, preferably fluoro.
19. The use, according to the previous claim, wherein R4 is hydroxy.
20. The use, according to anyone of the previous claims, wherein in the compound of formula I R5 is hydroxyalkyl.
21. The use, according to anyone of the previous claims, wherein in the compound of formula I R5 is hydroxymethyl, hydroxyethyl or hydroxypropyl, preferably hydroxymethyl.
22. The use, according to anyone of the previous claims, wherein in the compound of formula I R5 is hydroxymethyl.
23. The use, according to anyone of the previous claims, wherein in the compound of formula I R5 is hydroxymethyl or benzyloxymethyl, preferably hydroxymethyl.
24. The use of a compound of formula I defined in anyone of the preceding claims for the manufacture of a pharmaceutical composition for the treatment of diabetes.
25. The use of a compound of formula I defined in anyone of the preceding claims for the manufacture of a pharmaceutical composition inhibiting the glucose production from the liver.
26. A pharmaceutical composition containing a compound of formula I defined in anyone of the preceding claims in connection with a pharmaceutically acceptable carrier.
27. A method of treating diabetes which method comprises administering an effective amount of a compound of formula I
defined in anyone of the preceding claims to a patient in need of such a treatment.
defined in anyone of the preceding claims to a patient in need of such a treatment.
28. A method of inhibiting the liver glucose production from the liver which method comprises administering an effective amount of a compound of formula I defined in anyone of the preceding claims to a patient in need of such a treatment.
29. The use of a compound, according to anyone of the previous use, composition or method claims, which is 3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, 1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-propylpyrrolidine, 1-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, 1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, 3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, 1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, preferably 3,4-dihydroxy-2-hydroxymethylpyrrolidine and any of the optical isomers thereof.
30. The use of a compound, according to the previous claim, which is (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-methylpyrrolidine, (2R,3R,4R)-1-cyclopropylmethyl-3,4-di hydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-di-hydroxy-2-hydroxymethyl-1-propylpyrrolidine, (2R,3R,4R)-1-butyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)-pyrrolidine, (2R,3R,4R)-1-benzyl-3,4-dihydroxy-2-hydroxy-methylpyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxyprop-1-yl)pyrrolidine, (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-1-(1,3-dihydroxy-prop-2-yl)pyrrolidine, (2R,3R,4R)-1-(2-aminoethyl)-3,4-di-hydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-di-hydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-di-hydroxy-2-hydroxymethyl-1-methylpyrrolidine, (2S,3S,4S)-1-cyclopropylmethyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-propyl-pyrrolidine, (2S,3S,4S)-1-butyl-3,4-dihydroxy-2-hydroxy-methylpyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2,2,2-trifluoroethyl)pyrrolidine, (2S,3S,4S)-1-benzyl-3,4-dihydroxy-2-hydroxymethylpyrrolidine, (2S,3S,4S)-3,4-di-hydroxy-2-hydroxymethyl-1-(2-hydroxyethyl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxymethyl-1-(2,3-dihydroxy-prop-1-yl)pyrrolidine, (2S,3S,4S)-3,4-dihydroxy-2-hydroxy-methyl-1-(1,3-dihydroxyprop-2-yl)pyrrolidine, (2S,3S,4S)-1-(2-aminoethyl)-3,4-dihydroxy-2-hydroxymethylpyrrolidine, preferably (2R,3R,4R)-3,4-dihydroxy-2-hydroxymethyl-pyrrolidine.
31. Any novel feature or combination of features described herein.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK99295 | 1995-09-08 | ||
| DK0992/95 | 1995-09-08 | ||
| PCT/DK1996/000373 WO1997009040A1 (en) | 1995-09-08 | 1996-09-06 | 2-alkylpyrrolidines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2235415A1 true CA2235415A1 (en) | 1997-03-13 |
Family
ID=29403898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2235415 Abandoned CA2235415A1 (en) | 1995-09-08 | 1996-09-06 | 2-alkylpyrrolidines |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2235415A1 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9670195B2 (en) | 2012-08-31 | 2017-06-06 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
| US9695197B2 (en) | 2012-10-31 | 2017-07-04 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
| US9701693B2 (en) | 2011-06-27 | 2017-07-11 | Alectos Therapeutics Inc. | Selective glycosidase inhibitors and uses thereof |
| US9718854B2 (en) | 2011-03-31 | 2017-08-01 | Alectos Therapeutics Inc. | Selective glycosidase inhibitors and uses thereof |
| US9809537B2 (en) | 2012-08-31 | 2017-11-07 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
| US9815861B2 (en) | 2010-12-23 | 2017-11-14 | Alectos Therapeutics, Inc. | Selective glycosidase inhibitors and uses thereof |
-
1996
- 1996-09-06 CA CA 2235415 patent/CA2235415A1/en not_active Abandoned
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9815861B2 (en) | 2010-12-23 | 2017-11-14 | Alectos Therapeutics, Inc. | Selective glycosidase inhibitors and uses thereof |
| US9718854B2 (en) | 2011-03-31 | 2017-08-01 | Alectos Therapeutics Inc. | Selective glycosidase inhibitors and uses thereof |
| US9701693B2 (en) | 2011-06-27 | 2017-07-11 | Alectos Therapeutics Inc. | Selective glycosidase inhibitors and uses thereof |
| US9670195B2 (en) | 2012-08-31 | 2017-06-06 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
| US9809537B2 (en) | 2012-08-31 | 2017-11-07 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
| US9695197B2 (en) | 2012-10-31 | 2017-07-04 | Alectos Therapeutics Inc. | Glycosidase inhibitors and uses thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued | ||
| FZDE | Discontinued |
Effective date: 20030908 |