CA2235395A1 - Hybridation comparative d'acides nucleiques a ultra haute resolution appliquee a des fibres d'adn peignees - Google Patents

Hybridation comparative d'acides nucleiques a ultra haute resolution appliquee a des fibres d'adn peignees Download PDF

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Publication number
CA2235395A1
CA2235395A1 CA 2235395 CA2235395A CA2235395A1 CA 2235395 A1 CA2235395 A1 CA 2235395A1 CA 2235395 CA2235395 CA 2235395 CA 2235395 A CA2235395 A CA 2235395A CA 2235395 A1 CA2235395 A1 CA 2235395A1
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CA
Canada
Prior art keywords
dna
target
combed
nucleic acids
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2235395
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English (en)
Inventor
Aaron Bensimon
Thomas Cremer
Jurgen Kraus
Peter Lichter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Publication of CA2235395A1 publication Critical patent/CA2235395A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Cette invention concerne un procédé permettant de détecter la présence et de quantifier des acides nucléiques constituant une séquence dans un génome. Ledit procédé consiste (A) à immobiliser en la peignant la séquence d'acides nucléiques cible sur une matrice de support, (B) à mettre en contact la séquence d'acides nucléiques peignée avec un mélange, à proportions égales, d'une sonde d'acides nucléiques d'essai et d'une sonde d'acides nucléiques de référence, marquées différemment, de façon à provoquer l'hybridation, (C) à compter les points individuels d'hybridation marqués et localisés sur la séquence d'acides nucléiques peignée et (D) à déterminer le nombre d'acides nucléiques de la séquence à détecter et à quantifier en calculant le rapport entre le nombre total de points de la sonde d'essai et le nombre total de points de la sonde de référence.
CA 2235395 1995-11-13 1996-11-13 Hybridation comparative d'acides nucleiques a ultra haute resolution appliquee a des fibres d'adn peignees Abandoned CA2235395A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US663295P 1995-11-13 1995-11-13
US667895P 1995-11-14 1995-11-14
US60/006,632 1995-11-14
US60/006,678 1995-11-14

Publications (1)

Publication Number Publication Date
CA2235395A1 true CA2235395A1 (fr) 1997-05-22

Family

ID=26675874

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2235395 Abandoned CA2235395A1 (fr) 1995-11-13 1996-11-13 Hybridation comparative d'acides nucleiques a ultra haute resolution appliquee a des fibres d'adn peignees

Country Status (6)

Country Link
EP (1) EP0861331A1 (fr)
JP (1) JP2000500021A (fr)
AU (1) AU7329096A (fr)
CA (1) CA2235395A1 (fr)
NZ (1) NZ320546A (fr)
WO (1) WO1997018326A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2755147B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
FR2755149B1 (fr) * 1996-10-30 1999-01-15 Pasteur Institut Procede de diagnostic de maladies genetiques par peignage moleculaire et coffret de diagnostic
SE0101934D0 (sv) * 2001-06-01 2001-06-01 Pharmacia Ab New methods
CA2461878C (fr) * 2001-09-27 2012-04-03 Spectral Genomics, Inc. Procedes pour detecter des mosaicismes genetiques au moyen de reseaux
GB201208726D0 (en) * 2012-05-17 2012-07-04 Oxford Gene Tech Ip Ltd Methods and materials
WO2021222512A1 (fr) 2020-04-30 2021-11-04 Dimensiongen Dispositifs et procédés pour la manipulation macromoléculaire
US20230321653A1 (en) 2020-06-01 2023-10-12 Dimensiongen Devices and methods for cytogenetic analysis
WO2022035729A1 (fr) 2020-08-10 2022-02-17 Dimensiongen Dispositifs et procédés d'analyse de génome multidimensionnelle
WO2023055776A1 (fr) 2021-09-29 2023-04-06 Michael David Austin Dispositifs et procédés d'interrogation de macromolécules

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE205542T1 (de) * 1992-03-04 2001-09-15 Univ California Vergleichende genomhybridisierung
US5472842A (en) * 1993-10-06 1995-12-05 The Regents Of The University Of California Detection of amplified or deleted chromosomal regions
FR2716263B1 (fr) * 1994-02-11 1997-01-17 Pasteur Institut Procédé d'alignement de macromolécules par passage d'un ménisque et applications dans un procédé de mise en évidence, séparation et/ou dosage d'une macromolécule dans un échantillon.
US5830645A (en) * 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays

Also Published As

Publication number Publication date
EP0861331A1 (fr) 1998-09-02
NZ320546A (en) 1999-01-28
AU7329096A (en) 1997-06-05
WO1997018326A1 (fr) 1997-05-22
JP2000500021A (ja) 2000-01-11

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