CA2223519A1 - Procedes d'evaluation de cibles antimicrobiennes - Google Patents

Procedes d'evaluation de cibles antimicrobiennes Download PDF

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Publication number
CA2223519A1
CA2223519A1 CA 2223519 CA2223519A CA2223519A1 CA 2223519 A1 CA2223519 A1 CA 2223519A1 CA 2223519 CA2223519 CA 2223519 CA 2223519 A CA2223519 A CA 2223519A CA 2223519 A1 CA2223519 A1 CA 2223519A1
Authority
CA
Canada
Prior art keywords
gene
microbe
activity
level
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2223519
Other languages
English (en)
Inventor
Bret Benton
Keith Bostian
Pamela Contag
Molly B. Schmid
Thomas R. Parr, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Trine Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2223519A1 publication Critical patent/CA2223519A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Procédés d'évaluation de gènes microbiens en tant que cibles pour des composés qui inhibent la croissance d'un microbe ou modifient la pathogénèse d'un microbe, et d'évaluation de l'effet thérapeutique espéré de composés qui inhibent une réaction d'une cellule microbienne apparentée à l'expression d'un gène spécifique. Ces procédés mettent en application des microbes recombinés qui contiennent des produits de recombinaison ou des modifications d'ADN, de sorte que le niveau d'activité des produits de régions codantes associées à ces produits de recombinaison ou à ces modifications peut être commandé par mise en contact du microbe avec une petite molécule spécifique ou autre changement spécifique artificiel du milieu. Ces microbes recombinés peuvent être utilisés dans des modèles d'infection afin d'évaluer l'effet de la modification artificielle du niveau d'activité du produit d'un gène.
CA 2223519 1995-06-07 1996-05-28 Procedes d'evaluation de cibles antimicrobiennes Abandoned CA2223519A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48211895A 1995-06-07 1995-06-07
US08/482,118 1995-06-07

Publications (1)

Publication Number Publication Date
CA2223519A1 true CA2223519A1 (fr) 1996-12-19

Family

ID=23914751

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2223519 Abandoned CA2223519A1 (fr) 1995-06-07 1996-05-28 Procedes d'evaluation de cibles antimicrobiennes

Country Status (4)

Country Link
EP (1) EP0830457A1 (fr)
AU (1) AU5939996A (fr)
CA (1) CA2223519A1 (fr)
WO (1) WO1996040979A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7255851B2 (en) 1994-07-01 2007-08-14 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
US6217847B1 (en) 1994-07-01 2001-04-17 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive localization of a light-emitting conjugate in a mammal
WO1997023642A1 (fr) * 1995-12-22 1997-07-03 Microcide Pharmaceuticals, Inc. Technologie d'identification de marqueurs en fonction de la taille
US6846625B1 (en) * 1998-01-09 2005-01-25 Cubist Pharmaceuticals, Inc. Method for identifying validated target and assay combination for drug development
US6878516B1 (en) * 1998-01-16 2005-04-12 Pharmacia & Upjohn Company Autoregulatory system for validating microbial genes as possible antimicrobial targets using a tetracycline-controllable element
WO1999036554A1 (fr) * 1998-01-16 1999-07-22 Pharmacia & Upjohn Company Systeme autoregulateur de validation de genes microbiens en tant qu'eventuelles cibles antimicrobiennes utilisant un element regulable par la tetracycline
DE69933471T2 (de) 1998-12-17 2007-08-30 Xenogen Corp., Alameda Unblutige bestimmung einer physiologischen reaktion in einem säugetier
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6610503B1 (en) 1999-03-17 2003-08-26 Xenogen Corporation Animal models for predicting sepsis mortality
ES2260009T3 (es) 1999-05-05 2006-11-01 Phylogica Limited Aislamiento de moduladores biologicos a partir de bancos de fragmentos genicos biodiversos.
GB9915399D0 (en) * 1999-07-02 1999-09-01 Zeneca Ltd Method
US6737245B1 (en) 1999-09-08 2004-05-18 Xenogen Corporation Luciferase expression cassettes and methods of use
WO2001034810A2 (fr) 1999-11-09 2001-05-17 Elitra Pharmaceuticals, Inc. Genes indispensables a une proliferation microbienne et antisens a cet egard
US6867348B1 (en) 1999-12-16 2005-03-15 Xenogen Corporation Methods and compositions for screening for angiogenesis modulating compounds
IT1311335B1 (it) 1999-12-21 2002-03-12 Otc Optical Technologies Ct S Procedimento e dispositivo per realizzare reticoli in fibra ottica.
US7056728B2 (en) 2000-07-06 2006-06-06 Xenogen Corporation Compositions and methods for use thereof in modifying the genomes of microorganisms
EP1373496A4 (fr) * 2001-02-22 2004-10-27 Praecis Pharm Inc Procedes d'identification de peptides modulant un processus biologique
DK1987178T3 (en) 2006-02-20 2015-04-20 Phylogica Ltd Process for the construction and screening of peptide structure libraries
WO2023150176A1 (fr) * 2022-02-01 2023-08-10 Azitra Inc Expression du gène lekti humain à partir du chromosome de staphylococcus epidermidis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3853932D1 (de) * 1987-08-17 1995-07-13 Hoffmann La Roche Hochreprimierbare Expressionskontrollsequenzen.
FR2648149A1 (fr) * 1989-06-09 1990-12-14 Agronomique Inst Nat Rech Souches modifiees de bacteries phytophathogenes et leurs applications pour le criblage de molecules utilisables pour la protection des cultures
AU3825995A (en) * 1994-10-04 1996-04-26 New York University Blocking expression of virulence factors in s. aureus

Also Published As

Publication number Publication date
WO1996040979A1 (fr) 1996-12-19
EP0830457A1 (fr) 1998-03-25
AU5939996A (en) 1996-12-30

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