CA2223086A1 - Novel serpin derived from human hypothalamus - Google Patents

Abstract

The present invention provides nucleotide and amino acid sequences that identify and encode a novel serpin (CAPE) expressed in human hypothalamus. The present invention also provides for antisense molecules to the nucleotide sequences which encode CAPE, expression vectors for the production of purified CAPE, antibodies capable for binding specifically to CAPE, hybridization probes or oligonucleotides for the detection of CAPE-encoding nucleotide sequences, genetically engineered host cells for the expression of CAPE, a pharmaceutical composition containing biologically active CAPE, a diagnostic test based on CAPE-encoding nucleic acid molecules, and treatment methods comprising administration of biologically active CAPE.

Description

W O g~'4D322 PCT~US96/10179 NOVEL SERPIN DERIVED FROM HUMAN HYPOTHALAMUS

TECHNICAL FIELD
The present invention is in the field of molecular biology; more particularly, the present 5 invention describes the nucleic acid and amino acid sequences of a novel serpin cA~l~ssed in the hypoth~l~mllc, BACKGROUND ART
Inhibitory Serpins Serpins are irreversible serine protease inhibitors which are prinrip~lly located o extracellularly. As a group, they are defined on the basis of their structural and functional characteristics of a high molecular weight (bclwccll 370-420 amino acid residues), and a C-tPrmin~l reactive region. Proteins which have been ~cciFn~fl to the serpin family include a-l protease inhibitor, a-l-antichymotrypsin, ~,lilhlulllbin III, a-2-antiplasmin, heparin cofactor II, complement Cl inhibitor, pl~cminogen activator inhibitors 1 and 2, glia derived nexin, protein C
inhibitor, rat hepatocyte inhibitors, crmA (a viral serpin which inhibits interleukin l-n cleavage enzyme), human squamous cell calchlollla antigen which may modulate the host i....nl...~o uonse against tumor cells, human maspin which seems to function as a tumor ~u~lessor, lepidopteran ~l~otc~e inhibitor, leukocyte elastase inhibitor (the only known intracellular serpin), and products from three orthopoxviruses (these products may be involved in the regulation of the blood clotting cascade and/or of the complement cascade in the m~mm~ n host).
Serpins form tight complexes with their target proteases. The serpin region which binds to the target plvlcase is a mobile, exposed reactive site loop (RSL) which contains the Pl-Pl ' bond that is cleaved. When the rh~r~ct~ristic serpin Pl-Pl' bond cleaves, the serpin structure ch~ngPs profoundly, and stability to heat- or guanidine-in~l~lre~ dell;~luldlion increases m~rkellly.
These changes are referred to as the stressed-to-relaxed (S->R) transition, and are associated with tight complex formation with specific ~ teases. For the a l -~ruteillase inhibitor, cleavage of the Pl-Pl ' bond results in a separation of about 69A bclwccn the two residues (Loeberm~nn H et al (1984) J Mol Biol 177:531-556). The ability of a serpin to function as an inhibitor may be directly related to its ability to undergo this S->R transition (Bruch M et al (1988) J Biol Chem 263:16626-30; Carrell RW et al (I992) Curr Opin Struct Biol 2:438-446).
In addition, the RSL sequence from P 17 to P8 (hinge region) is highly conserved, and small amino acid with side chains are found at positions P9, P10, Pl l, P12, and P15 in active W O ~/40322 PCTAJS96/10179 inhibitors. The presence of small amino ~ids in this region allows the peptide loop from P14-P2 to be inserted into the middle of the protease inhibitor A-sheet. The insertion of this sequence into the A-sheet appears to be hllpol~l~ in stabilizing the inhibitor, and consequently tight~ning the protease/serpin complex. Sequence di~ nce in the hinge region may convert an inhibitor to a substrate.

NonInhibitory Serpins A number of proteins with no known inhibitory activity are also categori~d as serpins on the basis of strong sequence and structural similarities. These proteins can be cleaved by specific proteases, but do not form the tight complexes that inhibit plutease activity. Examples are bird ovalbumin, angiotensinogen, barley protein Z, corticosteroid binding globulin, thyroxine binding globulin, sheep uterine milk protein, pig uteroferrin-associated protein, an endopl~mic reticulum heat-shock protein (which binds strongly to collagen and could act as a cl~r~ e), pigment epithelium-derived factor, and an estrogen-regulated protein from Xenopus.
The nature of the di~.ence b~ ,.,n inhibitory and noninhibitory serpins is not well understood. For ~llple, ovalbumin is unable to undergo this S->R transition (Mottonen et al (1992) Nature 355: 270-273). However, hormone binding globulins, such as thyroxine or cortisol binding globulins, al~p~e.llly do undergo the transition from the native stressed to relaxed col~l.l.ation upon protease cleavage but do not form a tight complex with specific proteases (Pemberton et al (1988) Nature 336: 257-258). The S->R transition may confer an advantage for hormone binding molecules, and for small molecule binding proteins in general, in that the transition from a stressed to a relaxed col~llllalion may provide a method for mo~ ting hormone delivery. Both hormone binding globulins have a greater than 30%
homology with the archetype of the serpin family, alpha-l-antitrypsin, and sequence m~tt~.hing infers that they all share a common seco~ .y and tertiary structure.
Serpins are defined and described in Carrell R and Travis J (1985) Trends Biochem Sci 10:20-24; Carrell R et al (1987) Cold Spring Harbor Symp Quant Biol 52:527-535; Huber R and Carrell RW (1989) Bioçhtomi~try 28:8951-8966; and Remold-O'Donneel E (1993) FEBS Lett 315:105-108.
DISCLOSURE OF THE INVENTION
The subject invention provides a unique nucleotide sequence (cape) which encodes a WO 9~!4t~22 PCT~US96/10179 novel serpin (CAPE). The nucleotide sequence, which was identified from Incyte Clone 84476 derived from hypothalamic cells, contain3 two ATG codons. The two ATGs predict the ~,ession of the two different proteins: CAPE1 and CAPE2. However, only CAPEl includes a signal sequence. The subject invention includes the ~nticçn~e DNA of cape; cloning or 5 e~l"es~ion vectors co~ ;llg cape; host cells or ol~ c L~ rol",ed with e~l les~ion vectors colll;~ ing cape; a method for the production and recovery of purified CAPE polypeptide from host cells; purified CAPE polypeptide; antibodies to both polypeptides; and ph~ ological compounds using CAPE for the llc.~ of disease.
Furthermore, the subject invention also comprises diagnostic tests for pathologically o co"ll)r~ ised brain tissues including but not limited to the hypoth~l~rnllc which include the steps of testing a sample or an extract thereof with cape DNA, fr~gm~nt~ or oligomers thereof.

DESCRIPTION OF THE FIGURES
Figures lA, lB and 1C show the nucleotide sequence (SEQ ID NO:1) for cape inrl~ ing the entire coding sequence and the predicted amino acid sequences for CAPE1 and CAPE2 polypeptides (SEQ ID NO:2). The start codon for CAPE1 is at nucleotide 79; whereas the start codon for CAPE2 is at nucleotide 121.
Figures 2A, 2B, 2C, and 2D display the ~lignmlont of the CAPE molecules with plasminogen activator inhibitor 2 (PAI-2) (SEQ ID NO:3). Figures 2A-2B display the ~lignm~nt of CAPE1 with PAI-2 and Figures 2C-2D display the ~ nm~nt of CAPE2 with PAI-2. The majority sequences are consensus sequences (SEQ ID NO:4 and SEQ ID NO:5). ~lignmf-shown were produced using the ml~lti~equence ~lignm~nt program of DNASTAR software (DNASTAR Inc, Madison WI).
Figure 3 provides structural analysis of the cape sequence for d~ g putative alpha (A), beta (B), turn (T), and coil (C) regions; a hydrophilicity plot (H); alpha and beta i l.ip, l~ic regions (*); flexible regions (F); a putative antigenic index (AI); and a surface probability plot (S) using the structural analysis program of DNASTAR software (DNASTAR
Inc, Madison WI).

BRIEF DESCRIPTION OF DRAWINGS
Definitions As used herein, CAPE1 and CAPE2 refer to novel serpins, which may be naturally W O g~'4'~22 PCTAUS96tlO179 occurring or synthetically derived, or active fr~gmf nt~ thereof, which are çnl~oded by mRNAs transcribed from the cDNA (cape) of SEQ ID NO: 1. The amino acid sequence of CAPE 1 is shown in SEQ ID NO 2 starting at residue 1 and t~ g at residue 407, and that of CAPE2 is shown in SEQ ID NO 2 starting at residue 15 and tf rmin~ting at residue 407. As used herein, the term "CAPE" refers generally to both CAPEl and CAPE2.
"Active" refers to those forms of CAPE which retain biologic and/or immllnnlogicactivities of any naturally occ.lrring CAPE.
"Naturally occurring CAPE" refers to CAPE produced by human cells that have not been genf tiç~lly Pnginf f ~ed and specific~lly colllf;lllplates various forms arising from o post-translational modifications of the polypeptide, including but not limited to acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation.
"Derivative" refers to polypeptides derived from naturally occurring CAPE by chemical modifications such as ubiquitination, labeling (e.g., with radionuclides, various enzymes, chromogenic or fluorogenic means), pegylation (derivatization with polyethylene glycol), or by 15 insertion (or subaLiLuLion by ~hf~rnic~l synthesis) of amino acids such as ornithinf, which do not norm~lly occur in human ploleills.
As used herein the term "variant" or "recombinant variant" or "lllU~lL~ refers to any polypeptide differing from naturally occurring CAPE by amino acid (aa) insertions, deletions, and a~lb~ ;ons, created using recombinant DNA techniques. Guidance in d~L~ g which aa residues may be replaced, added or deleted without abolishing activities of interest, such as protein proteolysis, protease inhibition, or small molecule binding ~lop~;lLies, may be found by co~..p~. ;.,g the sequence of the particular CAPE with that of homologous inhibitory and noninhibitory serpins and minimi7ing the number of amino aicd sequence changes made in regions of high homology.
Plt;Ç~l~bly, amino acid "substitutions" are the result of replacing one amino acid with another amino acid having similar structural and/or C~f mic~l prol)c. Lies, such as the repl~cf .n~"l of a leucine with an isoleucine or valine, an aa~dlL~l~ with a gl~ r, or a Lhlcol~ e with a serine, i.e., conservative amino acid repl~cement~ "Insertions" or "deletions" are typically in the range of about 1 to 5 amino acids. The variation allowed may be ~ r~;,, ,f nt~lly detf rminf d by systematically making insertions, deletions, or substitutions of an amino acid in a CAPE
molecule using recombinant DNA techniques and assaying the resulting recombinant variants for activity.

WO ~6/4D322 PCTtUS96tlO179 Where desired, nucleic acid encoding a CAPE molecule can be g~nPtir~lly engin~pred to contain a "signal or a leader sequence" that can direct the polypeptide to a specific location in a cell or to a specific d~stin~tion outside of the cell. Such a sequence may be naturally present on the polypeptide of the present invention or provided from heterologous protein sources by 5 recombinant DNA techniques.
A polypeptide "fr~gm~nt," "portion," or "seg~ .L" is a stretch of amino acid residues which has sl-ffici~nt length to display biologic and/or immlm- genic activity on their own or when conjugated to a carrier protein such as keyhole limpet hemocyanin (KLH, Sigma). In a plefe.i~,d embodiment a fragment portion or segm~nt will contain at least about 5 amino acids, 10 often at least about 7 amino acids, typically at least about 8 to 13 amino acids, and, in various embo~i.n~ , at least about 17 or more amino acids. To be active, any CAPE polypeptide must have sufficient length to display biologic and/or immllnologic activity.
"Small molecules" are molecules with a molecular weight under 5000, more preferably under 2000. The small molecules of particular interest may be derived from the hypoth~l~ml~c7 15 such as oxytocin, vasop~ h~, dop~mine7 r,.,~ulJel~Lide Y, som~trJst~tin, or enkeph~linc These small molecules may directly affect the hypoth~l~mllc or other target neuronal tissues, such as the IJiluil~y gland. Alternatively, the small molecules may be derived from other tissues and affect the hypoth~l~mllc. These small molecules may include, but are not limited to, molecules such as s~,lulonill, epinephrine, nole~ ,k. ;..e, gamma arnino butyric acid, ghlt~m~te, or other 20 n~u,.~ll,.x. . .;l l~ . x or hormones. These small molecules may be naturally occ~lrring or synthetically made.
"Conditions ~cso~i~tPd with altered t~ Xxion of CAPE" refer to physiological or pathological changes of the hypoth~l~mllc or other tissues. Pathological changes include infl~mm~tion, disease and tumors.
"Hypothalamic tissue" refers to tissue derived mostly from the hypoth~l~ml-c, but which may include other tissue from organs that sullound or are ~dj~cçnt to the hypoth~l~m..c "Animal" as used herein may be defined to include human, domestic, or agricultural (cats, dogs, cows, sheep, etc.) or test species (mouse, rat, rabbit, etc.).
As used herein, an "oligonucleotide" or polynucleotide "fragment", "portion," or30 "segm~nt" refers to any stretch of nucleic acids encoding a CAPE molecule which is of sufficient length to use as a primer in polymerase chain reaction (PCR) or various hybridization procedures known to those of skill in the art, for the purpose of identifying or amplifying idPnti~l or related W O 9~'40~22 PCTrUS96/10179 nucleic acids.
The present invention includes purified CAPE polypeptides from natural or recombinant sources, vectors and host cells transformed with recombinant nucleic acid molecules encoding CAPE. Various methods for the isolation of the CAPE polypeptides may be accomplished by 5 procedures well known in the art. For example, such polypeptides may be purified by imm~ ffinity cl~ull~aLography by employing the antibodies provided by the present invention.
Various other m.othotlc of protein purification well known in the art include those described in Dc;~ rl~ . M (1990) Methods in Enzymology, Vol 182, Ar~dennic Press, San Diego; and Scopes R (1982) Protein Purification: Principles and Practice. Springer-Verlag, New York City, both 10 incol~ulaled herein by lcr~ nce.
As used herein the term "recombinant" refers to a polynucleotide which encodes CAPE
and is prepared using recombinant DNA techniques. The polynucleotide which encodes CAPE
may also include allelic or recombinant variants and .~ thereof.
As used herein the term "probe" or "nucleic acid probe" or "oligonucleotide probe" refers to a portion, r~ or segmrnt of a CAPE molecule that is capable of being hybridized to a desired target nucleotide seulu~"lce. A probe can be used to detect, amplify or quantify cDNAs or endogenous nucleic acid enrolling CAPE by employing conventional techniques in molecular biology. A probe may be of variable length, ~lcr~,lably from about 10 nucleotides up to several hu~ldl~d nucleotides. As will be understood by those of skill in the art, hybridization conditions 2û and probe design will vary depen-ling upon the int~ntled use. For example, a probe intrn(led for use in PCR will be from about 15 to 30 nucleotides in length and may be up to 60 nucleotides in length and may be part of a pool of deg.,~ probes, i.e., oligonucleotides which tolerate nucleotide micm~tcll but acc~ mmo-l~se binding to an unknown sequence; whereas a probe for use in Southern or northern hybridizations may be a single, specific nucleotide se4uence that is 25 several hundred nucleotides in length. Accordingly, a ple~llcd probe for the specific detection of a CAPE molecule will comprise a polynucleotide or oligonucleotide fragment from a non-conserved nucleotide region of SEQ ID NO: 1. As used herein the term "non-conserved nucleotide region" refers to a nucleotide region that is unique to SEQ ID NO: 1 and does not comprise a region that is conserved in the family of serpin genes. Probes may be single-stranded or double-stranded and may have specificity in solution, cell, tissue or membrane-based hybridizations including ~ and ELISA-like technologies. The present invention ~ .~rol~p~c~s oligonucleotides, fr~m~ntc or portions ofthe polynucleotides disclosed herein, or W O 9~1l4~22 PCT~US96/10179 their compl~ l y strands used as probes.
Nucleic acid probes may comprise portions of the sequence having fewer nucleotides than about 6 kb and usually fewer than about I kb. The oligonllrleti~Ps and nucleic acid probes of the present invention may be used to determinP, whether nucleic acid encoding CAPE is 5 present in a cell or tissue or to isolate identical or similar nucleic acid sequences from cl~ull~osomal DNA as clesrribed by Walsh PS et al (1992 PC R Methods Appl 1:241-250).
Nucleic acid probes of the present invention may be derived from naturally occ~-rring or col.lbh~ single- or double-stranded nucleic acids or be chemically synthPci7P-l They may be labeled by nick translation, Klenow fill-in reaction, PCR or other methods well known in the art.
10 Probes of the present invention, their ~ a~alion and/or l~b~ling are elaborated in Sambrook J et al (1989) Molecular Cloning: A Labolal~ly Manual, Cold Spring Harbor Laboratory, NY; or Ausubel FM et al (1989) Current Protocols in Molecular Biology, John Wiley & Sons, NYC, both incorporated herein by reference.
Alternatively, recombinant variants encoding the polypeptides of the present invention or related polypeptides may be synthP~i7Pcl or identifiç~l through hybridization techniques known to those of skill in the art by making use of the "red--n-l~n~y" in the genetic code. Various codon substitutions, such as the silent changes which produce various restriction sites, may be introduced to o~,lillli;~ cloning into a plasmid or viral vector or ~A~ ion in a particular prokaryotic or eukaryotic system. Mutations may also be introduced to modify the properties of 20 the polypeptide, to change ligand-binding affinities, i,ll~l~,haill ~ffinities, or polypeptide degradation or turnover rate.
Alternatively, recombinant variants encoding these same or similar polypeptides may be synthPsi7P~I or selected by making use of the "re~ll.ntl~n~y" in the genetic code. Various codon ;on~, such as the silent changes which produce various restriction sites, may be25 introduced to Optillli~ cloning into a plasmid or viral vector or c;~yl~ssion in a particular prokaryotic or eukaryotic system. Mutations may also be introduced to modify the properties of the polypeptide, including but not limited to small molecule-binding affinities, or polypeptide degradation or turnover rate. The nuc leotide sequence of recombinant variants can be ~letPr ninPcl by means known to those of skill in the art. Conventional en~;yl"alic methods employed DNA polymerase Klenow fr~gmPnt SEQUENASE~ (US Bioçhpmi~l Corp, Cleveland, OH) or Taq polymerase to extend DNA chains from an oligonucleotide primer ~nnP~Ie(l to the DNA template of interest. Methods have been developed for the use of both W O 9~ 22 PCTAJS96/10179 single- and double-stranded templates. The chain lf ~ ;on reaction products wereele.iLlu~holescd on urea-acrylamide gels and detected either by autoradiography (for radionuclide-labeled precursors) or by fluolcscellce (for fluolesc~nl-labeled pl~-,Ul:iUl:i). Recent improvements in ~.~ecl.A~ reaction pl~l,cllclLion, seqllPnring and analysis using the fluo-csc~
detection method have permitted expansion in the nurnber of sequences that can be ~ A
per day (using m~rhinrs such as the Catalyst 800 and the Applied Biosystems 377 or 373 DNA
sequencer).
The present invention includes purified CAPE polypeptide from natural or recombinant sources, and cells transforrned with recombinant nucleic acid molecules enrorling CAPE.
0 Various methods for the isolation of the polypeptide may be accomplieh~d by procedures well known in the art. For exarnple, such polypeptides may be purified by immnn~ffinity chromatography by employing the antibodies provided by the present invention. Various other methods of protein purification well known in the art include those described in Dellt~r~lPr M
(1990) Methods in Enzymology, Vol 182, ~c~-irmic Press, San Diego CA; and Scopes R (1982) Protein P~rific~tion: Principles and Practice. Springer-Verlag, New York City, both incol~(,lc Led herein by leI;~ ce.

Modes of Carryin~ Out the Invention The present invention provides a nucleotide sequence (cape) for a novel serpin, identified 20 in cDNA libraries made from hypothalamic cells, brain tissue and neonatal kidney cells . The sequence is provided in SEQ ID NO 1. Figs. lA-lC provide the cape nucleotide sequence, and the polypeptide sequence it encodes. The nucleotide sequence of SEQ ID NO: 1 contains two ~hern~tive start sites (ATG codons). These start sites serve to express two novel serpins which possess ~ub~Lall~ial overlap (> 95%) in the polypeptide sequence. One encoded protein (CAPE1) 2s is ~ cssed from an ATG codon at nucleotide position 79; its sequence is prese.lled in SEQ ID
NO 2 starting at residue 27. The second protein (CAPE2) is ~ ressed from an ATG codon at nucleotide position 121; its sequence is plese.l~ed in SEQ ID NO 2 starting at residue 41.
Figs. 2A-2D provide an ~lignmrnt of CAPE1 and CAPE2, re~l,cc~i~rely, with plasminogen activation inhibitor-2 (PAI-2), an exemplary serpin family member. CAPE1 30 contains a signal sequence coneieting of hydrophobic residues intlic~ting that it may be selectively llculayulL~d from hypothalamic cells to another location such as the ~iLuiLcuy gland.
;vely, CAPE2, like other serpins, may be secreted from hypothalamic cells. Overall, about 1 10 out of 406 residues of CAPE2 match exactly with those of PAI-2 (about 27%
homology). For the reactive site loop (RSL) residues P10, and P12-16 match exactly, v~ eas P8, P11, and P17 are ~llb~ lled by amino acids that are larger by either an extra carbon group, i.e. the pl-,sence of Ihleo~ e versus serine at P11, or a hydroxyl group, i.e. the presence of serine 5 versus alanine at P8.
Since CAPE appears to have an RSL that resembles that of inhibitory serpins, CAPE may inhibit llni~Pntified ,oroteases within or outside of cells. ~ l;v~ly, CAPE may serve to bind specific small molecules to Il~Ailll;1in higher levels of these molecules inside or outside of a cell and to modulate their release. Thel~rol~ CAPE, the novel serpin of the subject invention, may 10 function either to mask p~lease activity or to sequester small molecules.
In view of the fact that the cape nucleotide sequence has been i-iPntified in brain and hypothalamic cells, the nucleic acid (cape), polypeptide (CAPE), and antibody to CAPE may be useful in invçstig~tiQn~ of and the intervention in the normal and abnormal function of the numerous .on~loçrinP and nnnPn-locrine functions of the hypothAI~mlls. However, even though the cape sequence was found to be e,.~ s~ed in hypothalamic cells it should not be ruled out that cape may be t~pl~ ed in other cells, particularly other neuronal or secretory cells.
The nllcleotiflP seqllenre encoding cape has nullle.-~us applications in techniques known to those skilled in the art of molecular biology. These techniques include use as hybridization probes, use in the construction of oligomers for PCR, use for chromosome and gene mapping, 20 use in the recombinant production of CAPE and use in the generation of anti-sense DNA or RNA, their chemical analogs and the like. Uses of nucleotides encoding the proteins disclosed herein are P~Pnnpl~ry of known techniques and are not inten-lP~ to limit their use in any technique known to a person of oldii1~.y skill in the art. Furthermore, the nucleotide sequences disclosed herein may be used in molecular biology techniques that have not yet been developed, 25 provided the new techniques rely on properties of nucleotide sequences that are ~ nlly known, eg, the triplet genetic code, specific base pair interactions, etc.
It will be appreciated by those skilled in the art that as a result of the degeneracy of the genetic code, a mllltitll~le of CAPE-encoding nucleotide sequences, some bearing minim~l homology to the nucleotide sequence of any known and naturally occurring gene may be 30 produced. The invention has specifically contemplated each and every possible variation of nucleotide sequence that could be made by selecting combinations based on possible codon choices. These combinations are made in accordance with the standard triplet genetic code as CA 02223086 l997-l2-02 W O 96/1'322 PCT~US96/lOt79 applied to the nucleotide sequence of naturally occ~rring CAPE, and all such variations are to be con~idrred as being speçific~lly disclosed.
Although the nucleotide se.luences which encode CAPE and/or its variants are ylefclably capable of hybridizing to the nucleotide sequence of naturally oCcllrring CAPE under ~lh~ge, 5 conditions, it may be advantageous to produce nucleotide sequences encoding CAPE or its derivatives posses~ing a ~ lly di~GIGIlt codon usage. Codons can be selectec~ to increase the rate at which eAyl-,ssion of the peptide occurs in a particular prokaryotic or eukaryotic eAIJlGssion host in acco~ lce with the r~c.lu.,l~;y with which particular codons are utilized by the host. Other reasons for ~ ~b~ y altering the nucleotide sequence encoding CAPE and/or its 10 derivatives without altering the enr,o~F,~l amino acid sequence include the production of RNA
Lla~ scl;yl~ having more desirable propF.Lies, such as a greater half-life, than L a.,sc,;y~ produced from the naturally occurring sequence.
Nucleotide sequences encoding CAPE may be joined to a variety of other nucleotide sequences by means of well established recombinant DNA techniques (Sambrook J et al. supra).
5 Useful nucleotide sequences for joining to cape include an asso,ll"c.,l of cloning vectors, e.g., pl~cmi~l~, cosmi~ls, lambda phage derivatives, ph~gemitlc7 and the like, that are well known in the art. Vectors of interest include tAy,~ ion vectors, replication vectors, probe generation vectors, seql~rn~ing vectors, and the like. In general, vectors of interest may contain an origin of replication functional in at least one Ol~uliSlll, convenient restriction endon~clF~e sensitive 20 sites, and selectable markers for the host cell.
Another aspect of the subject invention is to provide for cape-specific nucleic acid hybridization probes capable of hybridizing with naturally occ~-rnng nucleotide sequences encoding CAPE. Such probes may also be used for the detection of similar serpin encoding se4u~,.,ces and should preferably contain at least 50% of the nucleotides from the conserved 25 region or active site. The hybridization probes of the subject invention may be derived from the nucleotide sequences of SEQ ID NO 1 or from genomic sequences including promoters, ellh~.rF el~mrnt~ and/or possible introns of lG!ipe~ili./e naturally occurring CAPE molecules.
Hybridization probes may be labeled by a variety of r.,pol ler groups, including radionuclides such as 32p or 35S, or el~ylll~lic labels such as ~lk~linto ph~ syh~ e coupled to the probe via avidin/biotin coupling systems, and the like.
Other means of producing specific hybridization probes for cape DNAs include thecloning of nucleic acid sequences encoding CAPE or CAPE derivatives into vectors for the W O 9~ 322 PCTAUS96/10179 prodllction of mRNA probes. Such vectors are known in the art and are colrull~re;ally available and may be used to synth~oei7l? RNA probes in vitro by means of the addition of the a~l~,p.;~le RNA polymer~e as T7 or SP6 RNA polymerase and the a~lu~l;ate radioactively labeled nucleotides.
It is now possible to produce a DNA sequence, or portions thereof, .onco~ling CAPE and their derivatives entirely by synthetic ch~mietry, after which the gene can be inserted into any of the many available DNA vectors using rç~g~ntc vectors and cells that are known in the art at the time of the filing of this application. Moreover, synthetic ch~mietry may be used to introduce mutations into the cape se.lucnces or any portion thereof.
0 PCR as described in US Patent Nos 4,683,195; 4,800,195; and 4,965,188 provides additional uses for oligonucleotides based upon the nucleotide sequence which encodes CAPE.
Such probes used in PCR may be of recombinant origin, may be chemically synthPci7.q~1 or a llli~luie of both and comprise a discrete nucleotide seql)çnce for diagnostic use or a dege,l~.dle pool of possible sequences for identification of closely related genomic sequences.
Full length genes may be cloned from known sequence using a new method which employs XL-PCR (Perkin-Elmer, Foster City, CA) to amplify long pieces of DNA as disclosed in Guegler, United States Application Serial Number 08/487,112 filed June 7, 1995. This method was developed to allow a single l~seal-,~r to process multiple genes (up to 20 or more) at a time and to obtain an eYt~nlled (possibly full-length) sequence within 6-10 days. It replaces current methods which use labelled probes to screen libraries and allow one l~seal~;her to process only about 3-5 genes in 14-40 days.
In the first step, which can be pelrolllled in about two days, primers are dçeign~d and synth~ei7ed based on a known partial sequence. In step 2, which takes about six to eight hours, the sequ~,.lce is eytçn~ by PCR amplification of a selected library. Steps 3 and 4, which take about one day, are pnrific~tion of the amplified cDNA and its ligation into an a~,l.,pliate vector.
Step 5, which takes about one day, involves transforming and growing up host bacteria. In step 6, which takes ~ hnately five hours, PCR is used to screen b~ctçri~l clones for extended sequence. The final steps, which take about one day, involve the ,olel~dldlion and sequencing of selçcted clones. If the full length cDNA has not been obtained, the entire procedure is repeated using either the original library or some other plcr~ d library. The pler~.lcd library may be one that has been size-select~d to include only larger cDNAs or may consist of single or combined co~ ;ially available libraries, eg. lung, liver, heart and brain from Gibco/BRL (Gaithersburg W O 96/40922 PCT~US96/10179 MD). The cDNA library may have been prepared with oligo dT or random primers. The advantage of using random primed libraries is that they will have more sequences which contain 5' ends of genes. A randomly primed library may be particularly useful if an oligo dT library does not yield a complete gene. Obviously, the larger the protein, the less likely it is that the complete gene will be found in a single plasmid.
The nucleotide sequence disclosed in SEQ ID NO: 1 can be used in an ~say to detect conditions associated with altered GA~ ion of CAPE. The nucleotide sequence can be labeled by methods known in the art and added to a fluid or tissue sample from a patient under hybridizing conditions. After an incubation period, the sample is w~hed with a compatible fluid 0 which optionally contains a dye (or other label lc.lui~ g a developer) if the nucleotide h~ been labeled with an enzyme. After the colll~alible fluid is rinsed off, the dye is .lu~llildled and cG,l,paled with a standard. If the amount of dye is significantly elevated, the nucleotide sequence has hybridized with the sample, and the ~say indicates the plescllce of infl~mm~tion, tumor and/or dise~e.
The nucleotide se~luG"ce for cape can be used to construct hybridization probes for mapping that gene. The nucleotide sequence provided herein may be mapped to a particular chromosome or to specific regions of that chromosome using well known genetic and/or chromosomal mapping techniques. These techniques include in situ hybridization, linkage analysis against known chromosomal markers, hybridization sclc~nillg with libraries, flow-sorted 20 chromosom~l plGp~alions~ or artificial chromosome constructions YAC or Pl constructions.
The technique of fluGlcscenl in situ hybridization of chromosome spreads has been described, among other places, in Verma et al (1988) Human Chromosomes: A Manual of B~ic Techniques, Pergamon Press, New York City.
Fluorcsccl,l in situ hybridization of chromosomal p~ ,~dlions and other physical2s chromosome mapping techr~iques may be correlated with additional genetic map data. Examples of genetic map data can be found in the 1 994 Genome Issue of Science (265:1 98 1f). Correlation bGlweG" the location of cape on a physical chromosomal map and a specific dise~e (or predisposition to a specific dise~e) can help delimit the region of DNA ~sociated with that genetic dise~e. The nucleotide sequence of the subject invention may be used to detect 30 dirr~lcllces in gene sequence between normal and carrier or affected individuals.
Nucleotide se4u~llces encoding CAPE may be used to produce purified CAPE using well known m~tho~1c of recombinant DNA technology. Among the many publications that teach W O 9~/~C~22 PCT~US96/10179 methods for the ~ ion of genes after they have been isolated is Goeddel (1990) Gene Expression Technology, Methods and Enzymology, Vol 185, Ac~lPmic Press, San Diego CA.
CAPE may be e~plessed in a variety of host cells, either prokaryotic or ~uk~ ~ulic. Host cells may be from the same species in which cape nucleotide se-luences are endogenous or from a dirrti-ell, species. Advantages of producing CAPE by recombinant DNA technology include obtaining adequate amounts of the protein for purification and the availability of simplified purification procedures.
Cells transformed with DNA encoding CAPE may be cultured under conditions suitable for the eA~lession of serpins and recovery of the protein from the cell culture. CAPE produced 10 by a recombinant cell may be secreted or may be contained intr~ce~ rly, del,ellding on the cape sequence and the genetic col~l,u.;Lion used. In general, it is more convenient to prepare recombinant proteins in secreted form. Purification steps vary with the production process and the particular protein produced.
In addition to recombinant production, fr~gmPnte of CAPE may be produced by direct peptide synthesis using solid-phase techniques (cf Stewart et al (1969) Solid-Phase Peptide Synthesis, WH Freeman Co, San Francisco CA; Merrifiçl~l J (1963) J Am Chem Soc 85:2149-2154. In vitro protein synthesis may be p~,lrullllcd using manual techniques or by ~ululllalion. AulolllaLed synthesis may be achieved, for eA~ull~; le, using Applied Biosystems 431A Peptide Syt theei7Pr (Foster City, California CA) in accoldallce with the h~llu;lions 20 provided by the m~nllf~- tllrer. Various fr~gmPntc of CAPE may be chemically synth~ei7~d separately and combined using chemical m~thotlc to produce the full length molecule.
CAPE for antibody induction does not require biological activity; however, the protein must be ~ntigenic Peptides used to induce specific antibodies may have an amino acid sequence consisting of at least five amino acids, preferably at least 10 amino acids. They should mimic an 25 exposed portion of the amino acid sequence of the protein and may contain the entire amino acid sequence of a small naturally occurring molecule such as CAPE. Short stretches of CAPE amino acid may be fused with those of another protein such as keyhole limpet hemocyanin and the resnlting chimeric molecule used for antibody production.
Antibodies specific for CAPE may be produced by inoculation of an al,plol,liate animal 30 with the polypeptide or an antigenic fr~gm~nt An antibody is specific for CAPE if it is produced against an epitope of the polypeptide and binds to at least part of the natural or recombinant protein. Antibody production includes not only the stimulation of an immnnP

W O ~'4-~22 PCT~US96/10179 onse by injection into ~nim~lc, but also analogous steps in the production of synthetic antibodies or other specific-binding molecules such as the s~ ning of recombinant imml-nf)glQbulin libraries (Orlandi R et al (1989) PNAS 86:3833-3837, or Huse WD et al (1989) Science 256: 1275-1281) or the in vitro stimul~tion of lymphocyte populations Current technology (Winter G and Milstein C (1991) Nature 349:293-299) provides for a number of highly specific binding reage.,l~ based on the 1~ h~;iples of antibody formation. These techniques may be adapted to produce molecules specifically binding CAPE.
Two approaches are utilized to raise antibodies to CAPE, and each ap~"~ach is useful for gcll~,dLi~lg either polyclonal or monoclonal antibodies. In one a~roacl1, dclldlul~d protein from o the reverse phase HPLC separation is obtained in q~ntities up to 75 mg. This denatured protein can be used to imm--ni7P mice or rabbits using standard protocols; about 100 micrograms are adequate for immunization of a mouse, while up to 1 mg might be used to ;~ a rabbit.
For identifying mouse hybridomas, the denatured protein can be radioictlin~te~l and used to screen potential murine B-cell hybridomas for those which produce antibody. This procedure I~ uheS only small q~ntities of protein, such that 20 mg would be sufficient for labeling and sc,~,eni"g of several thousand clones.
In the second approach, the amino acid sequence of CAPE, as cled~ed from translation of th~e cDNA, is analy~d to dc t. ~ o regions of high immlmogenicity Oligopeptides comprising regions which are hydrophilic, highly ~ntigenic~ or highly likely to be on the serpin surface, as shown in Fig. 3, are synthPci7~d and used in suitable i.. ~ ion protocols to raise antibodies. Analysis to select apl)lu~liale c~ilopes is described by Ausubel FM et al (supra).
The optimal amino acid sequences for ;~ n are usually at the C-l~ ...;..llc, the N-t~....;....c and those intervening, hydrophilic regions ofthe polypeptide which are likely to be exposed to the çxt~rn~l c.,vh."""ent when the protein is in its natural conformation.
Typically, selecte~l peptides, about 15 residues in length, are syntheci7~d using an Applied Biosystems Peptide Synthlosi7~or Model 43 lA using fmoc--~h~mictry and coupled to keyhole limpet hemocyanin (KLH) by reaction with M-m~l,qimidoben-oyl-N-hydroxysucch1i",ide ester (MBS; cf. Ausubel FM et al, supra). If nPcec~i;..y, a cysteine may be introduced at the N-t~ ....i,,.lc of the peptide to permit coupling to KLH. Rabbits are immunized 30 with the peptide-E~LH complex in complete Freund's adjuvant. The res~lting antisera are tested for a"li~ tide activity by binding the peptide to plastic, blocking with 1% BSA, reacting with antisera, washing and reacting with labeled (radioactive or fluo,~,scelll), affinity purified, specific W O 96/40922 PCT~US96/10179 goat anti-rabbit IgG.
Hybridomas may also be pl~pdled and sclee.led using standard techniques. Hybridom~c of interest are dçtectecl by sclcenillg with labeled CAPE to identify those fusions producing the monoclonal antibody with the desired specificity. In a typical protocol, wells of plates (FAST;
Becton-Dickinson, Palo Alto CA) are coated with affinity purified, specific rabbit-anti-mouse (or suitable anti-species Ig) antibodies at 10 mg/ml. The coated wells are blocked with 1% BSA, washed and exposed to ~u~ from hybridomas. After inc~lb~tion the wells are exposed to labeled CAPE, 1 mg/ml. Clones producing antibodies will bind a ~lu~ltily of labeled CAPE
which is cletect~hle above background. Such clones are exp~ndPd and subjected to 2 cycles of 10 cloning at limiting dilution (1 cell/3 wells). Cloned hybridomas are injected into pristane mice to produce ascites, and monoclonal antibody is purified from mouse ascitic fluid by affinity chromatography on Protein A. Monoclonal antibodies with affinities of at least 1 o8 M-', preferably 109 to 10'~ or stronger, will typically be made by standard procedures as described in Harlow and Lane (1988) Antibodies: A Labolalol.y Manual. Cold Spring Harbor Labola~~~5 Cold Spring Harbor NY; and in Goding (1986) Monoclonal Antibodies: Principles and Practice, d~mic Press, New York City, both incol~uldled herein by lefcl, .lce.
An additional embodiment of the subject invention is the use of CAPE as a specific plOleaSe inhibitor to treat infl~mm~tory or pathologic problems of the hy~oll.AI~mllc, or another target tissue. A further embodiment of the subject invention is the use of CAPE to specifically bind a small molecule and to modul~te its release either within the hypothalamus, a target tissue or extracellularly.
CAPE as a bioactive agent or composition may be ~minictered in a suitable thcld~ ic dose det~ d by any of several methodologies including clinical studies on ~ .."~ n species to drte ..~ m~xim~l tolerable dose and on normal human subjects to detennin~ safe dose. Additionally, the bioactive agent may be complexed with a variety of well established compounds or compositions which ~nh~nre stability or ph~ ological properties such as half-life. It is colllelllplated that the theldl)culic, bioactive composition may be delivered by intravenous infusion into the bloodstream or any other effective means which could be used for treating problems involving excess c~lc~sion and activity of proteases. Alternatively, the 30 compositions may be employed for treating problems associated with excessive levels of specific small molecules.
Since CAPE appears to be a ~ tease inhibitor, it may be used to treat ~ eS associated W O 9".C3~2 PCT~US96/10179 with it GA~Jles~ion such as, tissue wasting associ~ted with excessive protease production during infl~mm~tion or tliee~çs associa~Gd with nervous tissue degGn~,lalion. The tissues that may be ~ffectecl by wasting are the brain or hypoth~l~ml~c7 where the serpin may be eAlJlessed in tissues surrounding or adjacPnt to the hypoth~l~mll~ For example, r,cu,ollal loss associated with 5 ~ e~ces such as K~llm~nn's and Down's syndromes, or in ~l7hPimsr's and Huntington's ~lice~ces may be plGvG.llGd by ~Amini~tration of CAPE molecules. Destruction of the posterior hypoth~l~m..c by i~chPmi~ encGph~liti~, trauma or tumor may also be prevented byatlmini~tration of CAPE molecules..
CAPE also appears to be a small molecule binding protein which can be used to modulate 10 levels of specific small molecules in the ll~allllClll of disease. For example, anorexia, bulimia, depression, and some forms of diabetes may be related to the overproduction of one or more of the molecules, such as CRH, ACTH, TRH, TSH, GRH, GH, insulin, som~t-lstatin, cholecystokinin, interleukins, oxytocin, insulin-like growth factors, glucagon, etc., which govern the nonendocrine intake and eating behaviors. CAPE may be employed to bind one of these molecules, thereby de~ asillg the symptoms of these ~liee~çc For thcla~Gulic uses, CAPE will be formnl~ted in a nontoxic, inert, ph~rm~elltic~lly acceptable aqueous carrier medium preferably at a pH of about S to 8, more ~Jl. felàbly 6 to 8, although the pH may vary acco~dillg to the cLa~ t~ . ;.ctics of the formulation and its ~-lmini.ctr~tion. Clla~ tics such as solubility of the molecule, half-life and antigenicity/;.. ~ ogenicity will aid in defining an effective carrier. Native CAPE human proteins are prGrGlled as a tre~tmPntc for ~ e~cp~s~ but recombinant, organic or synthetic CAPE
molecules may be equally effective in particular situations.
CAPE may be delivered by known routes of atlminictration including but not limited to llA .~ cQs~l spray and aerosol, tr~nederm~l patch and bandage, intravenous formulations, orally 25 ~lmini~tpred li(luids and pills particularly formnl~ted to resist stomach acid and enzymes. For higher specificity in ~lmini~tration, CAPE may be directly injected or impl~ntP~d in the brain, close to the hypoth~l~mnc The particular formulation, exact dosage, and route of ~lmini~tration will be ~letermin~d by the ~ttPn~ing physician and will vary according to each specific situation. Such 30 ~l~t~ ;ons are made by considering multiple variables such as the condition to be treated, the CAPE molecule to be ~-lminictPred, and the ph~. ,.,~rokinetic profile of the particular CAPE
molecule. Additional factors which may be taken into account include disease state (e.g.

W O 96/40922 PCTrUS96/10179 severity) of the patient, age, weight, gender, diet, time of ~ lion, drug combination, reaction sensitivities, and tolerance/f~ onse to therapy. Long acting formulations might be ~rlmini~tered every 3 to 4 days, every week, or once every two weeks depending on half-life and cle~r~nre rate of the particular molecule.
s Normal dosage amounts may vary from 0.1 to 100,000 micrograms, up to a total dose of about 1 g, depending upon the route of ~lminietration. Guidance as to particular dosages and methods of delivery is provided in the lilGIdlulz; see US Patent Nos. 4,657,760; 5,206,344; or 5,225,212. It is ~nticip~ted that dirr~,.ell- formulations will be effective for dirr~Gnl uses of CAPE and that ~tlminietration targeting a tissue or organ may neces~ilalG delivery in a specific manner.
The examples below are provided to illustrate the subject invention. These e~unl,lcs are provided by way of illustration and are not inrlllfied for the purpose of limiting the invention.

EXAMPLES
I Isolation of mRNA and Construction of cDNA Libraries The hypothalamic library was constructed from a pooled sample of hypothalamic tissue taken from the normal human brains of 51 C~ ci~n males and females of dirr~ - ages. The polyadenylated mRNA w~ obtained from Clontech Lab~ldl~u;cs~ Inc. (Catalogue No. #6579-2, Palo Alto CA) The polyadenylated mRNA was used to construct a custom cDNA library (Stratagene, La Jolla CA). cDNA ~yll~le3is was primed using both oligo dT and random he~mers, and the two cDNA libraries produced were treated s~ al~ly. Synthetic adapter oligonucleotides were ligated onto the cDNA enabling its insertion into the Stratagene Uni-ZAPTM vector system. This system allows high efficiency unidirectional (sense orientation) lambda library construction and the convenience of a pl~emi~l system with blue/white color selection to detect clones with cDNA
insertions. Finally, the two cDNA libraries were combined into a single library by mixing equal llWllbe,~S of bacteriophage.
The hypothalamic cDNA library is screened with either DNA probes or antibody probes - and the pBhles~ ,t¢ phagemid (Stratagene) can be rapidly excised in vivo. The phagemid allows the use of a plasmid system for easy insert ch~udcl~ dlion, seqlleneing, site directed mutagenesis, creation of unidirectional deletions, and e~l.lession of fusion proteins. The custom-col~llu.;led library phage particles were infected into E. Coli host strain XLl Blue0 W O ~ 22 PCT~US96/10179 (Stratagene) which has a high l~ rc,llllation efficiency. This efficiency ill.,l~ases the probability of obt~ilmlg rare, under-lel l~s~ ed clones in the cDNA library. Alt~,.l~live unidirectional vectors include but are not limited to pcDNAI (Invitrogen, San Diego CA) and pSHlox-1 (Novagen, Madison WI).

II Ir~l t1~n of cDNA Clones The ph~g~ornid forms of individual cDNA clones were obtained by the in vivo e~ci~ n process, in which XL1-BLUE wac coil~;led with an fl helper phage. Plot.,ins derived from both lambda phage and fl helper phage initi~ted new DNA synthesis from defined sequences on o the lambda target DNA and create a smaller, single-stranded circular phagemid DNA molecule that includes all DNA sequences of the pBlues~;fi~t plasmid and the cDNA insert. The phagemid DNA wa released from the cells and purified, then used to reinfect fresh b~cteri~l host cells (SOLR, Stratagene Inc), where the double-stranded ph~ge...i~l DNA was produced. Because the ph~g~mid carries the gene for B-l~ ce, the newly l,d,l~rulll,cd b~rteri~ were selected on I,lediul" co~ p ampicillin.
Phagemid DNA was purified using the QIAWELL-8 Plasmid Purification System0 (QIAGEN Inc, Chal~wollh CA). This technique provides a rapid and reliable high-throughput method for lysing the b~cttori~l cells and isolating highly purified phagemid DNA. The DNA
eluted from the purification resin was suitable for DNA sequencing and other analytical manipulations.
An alternate method of purifying phagemid has recently become available. It utilizes the Miniprep Kit (Catalog No. #77468, Advanced Genetic Technologies Corporation, Gaithel~
MD). This kit is in the 96-well format and provides enough reagents for 960 purifications. Each kit is provided with a leco.. - ~-rle~l protocol, which has been employed except for the following changes. First, the 96 wells are each filled with only 1 ml of sterile terrific broth with carb-onicillin at 25 mg/L and glycerol at 0.4%. After the wells are inoc~ te~ the bacteria are cultured for 24 hours and lysed with 60 ~1 of lysis buffer. A centrifugation step (2900 rpm for 5 ~..i...~les) is performed before the collle,ll~ ofthe block are added to the primary filter plate. The optional step of adding isopropal~ol to TRIS buffer is not routinely performed. After the last step ~o in the protocol, samples are "dl~r~ ,d to a Ber~m~n 96-well block for storage.

III Sequencing of cDNA Clones W O ~6/4C~22 PCTAUS96/10179 The cDNA inserts from random isolates of the hypoth~l~mtl~ library were sequenced in part. Methods for DNA sequencing are well known in the art. The cDNAs were sequenced by the method of Sanger F. and AR Coulson (1975; J. Mol. Biol.94:441f), using a Hamilton Micro Lab 2200 (Hamilton, Reno NV) in combination with four Peltier Thermal Cyclers (PTC200 from MJ Research, Watertown MA) and Applied Biosystems 377 or 373 DNA Sequencing Systems (Perkin Elmer) and reading frame ~letPrTninP~l IV Homology Searching of cDNA Clones and Deduced Proteins Each sequence so obtained was col~lpaled to sequences in GenR~nk using a search algorithm developed by Applied Biosystems Inc. and incoll,ola~d into its INHERITTM 670 Sequence Analysis System. In this algorithm, Pattern Specification Language (developed by TRW Inc.) was used to del~,llline regions of homology. The three parameters that ~Ptermin~
how the sequence comparisons run were window size, window offset, and error tolerance. Using a combination of these three pala~neters~ the DNA ti~t~b~e was searched for sequences CQl~ g regions of homology to the query sequence, and the ~ rul,l;al~ sequences were scored with an initial value. Subsequently, these homologous regions were e~minPd using dot matrix homology plots to distinguish regions of homology from chance m~t~hPs Smith-W~t~rm~n ~ligi~ t'i of the protein sequence were used to display the results of the homology search.
Peptide and protein sequence homologies were asc~" lained using the INHERIT 670 Sequence Analysis System in a way similar to that used in DNA sequence homologies. Pattern Specification Language and p~ulleter windows were used to search protein d~t~baees for sequences co~ g regions of homology which were scored with an initial value. Dot-matrix homology plots were examined to distinguish regions of significant homology from chance m~trh~s.
Alternatively, BLAST, which stands for Basic Local Alignmtont Search Tool, is used to search for local sequence ~lignmPrltc (~It~hlll, SF (1993) J Mol Evol 36:290-300; ~It.~c.hnl, SF
et al (1990) J Mol Biol 215:403-10). BLAST produces alignments of both nucleotide and amino acid sequences to ~ e sequence similarity. Because of the local nature of the alignments, BLAST is especi~lly useful in determining exact m~t~hPs or in identifying homologues.
Although it is ideal for m~t~hPs which do not contain gaps, it is hla~ opliate for performing motif-style se~;l~lg. The filn-l~mPnt~l unit of BLAST algorithm output is the high-scoring W O gf'~0~22 PCTAJS96/10179 se~ pair (HSP).
An HSP consists of two sequence fr~gm~ntc of all~iLIal~ but equal lengths whose ~lignmPnt is locally m~Yim~l and for which the ~ nm~nt score meets or exceeds a threshold or cutoff score set by the user. The BLAST approach is to look for HSPs between a query seqll~n-~e 5 and a ~t~h~ce sequence, to evaluate the statistical significance of any m~tches found, and to report only those m~tchPs which satisfy the user-selected threshold of significance. The p~alll.,tel E establishes the statistically significant threshold for repo, Ling ~l~t~h~ce sequence m~trh~s E is hlte~ eted as the upper bound of the expected frequency of chance oc~ ,l,ce of an HSP (or set of HSPs) within the context of the entire tl~t~b~ce search. Any rl~t~b~ce sequence 10 whose match satisfies E is reported in the program output.

V Identification and Full Length Sequencing of the Genes The nucleotide sequence for the entire coding region of CAPE is shown in Figs. l A- l C.
The cDNA of Incyte 84476 was PYtPn~1Pd to full length using a modified XL-PCR (Perkin Elmer) procedure. Primers were cleci~nPd based on known sequence; one primer w~
~y--lt-~ d to initiate extension in the ~nticpnce direction (XLR) and the other to extend sequence in the sense direction (XLF). The se4uences of these primers and their location are as follows: XLR (nucleotides 502-525 in SEQ ID NO 1) and XLF (nucleotides 609-632 in SEQ
ID NO l ) . The primers allowed the sequence to be extended "outward" generating amplicons 20 co..l;.;~ g new, unknown nucleotide sequence for the gene of interest (Guegler supra). The primers were ~1esignPd using Oligo 4.0 (National Biosciences Inc, Plymouth MN) to be 22-30 nucleotides in length, to have a GC content of 50% or more, and to anneal to the target sequence at t~ alul~s about 68 ~-72~ C. Any stretch of nucleotides which would result in hairpin ul~,S and primer primer .l;.,.- . ;7~ ons were avoided.
2s The hypoth~l~mllc cell cDNA library was used as a tPmpl~te, and XLR and XLF primers were used to extend and amplify the 84476 sequence. By following the instructions for the XL-PCR kit, the enzymes provided high fidelity in the amplification. Beginning with 25 pMol of each primer and the Iccolllll~on~e~ collccll~ ions of all other COlllpOll~ of the kit, PCR was p.,lrolllled using the MJ PTC200 (MJ Research, Watertown MA) and the following parameters:
Step 1 94~ C for 60 sec (initial den~ula~ion) Step 2 94~ C for 15 sec Step 3 65 ~ C for l min Step 4 68~ C for 7 min W O 9C/~C~22 PCT~US96/10179 Step 5 Repeat step 2-4 for 15 additional cycles Step 6 94~ C for 15 sec Step 7 65 ~ C for 1 min Step 8 68~ C for 7 min + 15 sec/cycle Step 9 Repeat step 6-8 for 11 additional cycles Step 10 72 ~ C for 8 min Step 11 4 ~ C (and holding) At the end of 28 cycles, 50 ~1 of the reaction mix w~ removed; and the rem~inin reaction mix w~ run for an additional 10 cycles ~ outlined below:
0 Step 1 94~ C for 15 sec Step 2 65~ C for 1 min Step 3 68~ C for (10 min + 15 sec)/cycle Step 4 Repeat step 1-3 for 9 additional cycles Step 5 72~ C for 10 min A 5- l O ,ul aliquot of the reaction mixture w~ analyzed by electrophoresis on a low conc~ alion, about 0.6-0.8%, agarose mini-gel to ~let~ which reactions were succec.cful in çxt~nfiing the sequence. Although all extensions potentially contain a full length gene, some of the largest products or bands were selected and cut out of the gel. Further purification involved using a coll"ll~,.cial gel extraction method such as QIAQuickTM (QIAGEN Inc, Chal~wullh CA).
2û After recovery of the DNA, Klenow enzyme w~ used to trim single stranded, nucleotide overhangs creating blunt ends which f~rilit~t~i religation and cloning.
After ethanol y~ ion, the products were redissolved in 13 ~41 of ligation buffer.
Then, 1~1 T4-DNA lig~e (15 units) and 1~1 T4 polynucleotide kin~e were added, and the mixture w~ incub~te~l at room tem~ Lu,c for 2-3 hours or overnight at 16~ C. Colllyelclll E.
coli cells (in 40 ~1 of ayyn~yliate media) were transformed with 3 ~1 of ligation mixture and cultured in 80,ul of SOC medium (Sambrook J et al, supra). After inrub~tion for one hour at 37 C, the whole ~ rollllation mixture w~ plated on Luria Bertani (LB)-agar (Sambrook J et al, supra) col~ carbenicillin at 25 mg/L. The following day, 12 colonies were randomly picked from each plate and cultured in 150,ul of liquid LB/carbenicillin medium placed in an individual well of an apyloyliate~ coll"ll~,lcially-available, sterile 96-well microtiter plate. The following day, 5 ~1 of each overnight culture w~ transferred into a non-sterile 96-well plate and after dilution 1: 10 with water, 5,ul of each sample was transferred into a PCR array.
For PCR ~mplifiç~tion, 15 ~1 of PCR mix (1.33x co--l;~;-,;.~g 0.75 units of Taq polymer~e, a vector primer and one or both of the gene specific primers used for the extension reaction) were added to each well. Amplification w~ p~lroll"ed using the following conditions:

Step 1 94~ C for 60 sec Step 2 94~ C for 20 sec Step 3 55 ~ C for 30 sec Step 4 72~ C for 90 sec Step 5 Repeat steps 2-4 for an additional 29 cycles Step 6 72~ C for 180 sec Step 7 4~ C (and holding) Aliquots of the PCR reactions were run on agarose gels together with molecular weight markers. The si~s of the PCR products were colllpaled to the original partial cDNAs, and a~ opl;ate clones were selecte~ ligated into plasmid and sequenced.

VI Antisense analysis Knowledge of the cDNA sequence of the novel serpin gene will enable its use in ~nti~ence technology in the investigation of gene function. Oligonucleotides, genomic or cDNA
fr~gm~ntc comprising the ~nticrnce strand of cape are used either in vitro or in vivo to inhibit cA~lGssion of the protein. Such technology is now well known in the art, and probes can be ~ecign~cl at various locations along the nucleotide sequence. By llallsrcclion of cells or whole test ~nim~lc with such ~nticrnce se4u~.~ces, the gene of interest can effectively be turned off.
Frequently, the function of the gene can be ascc,lahled by observing behavior at the cellular, tissue or org~nicm~l level (e.g. Iethality, loss of dirr~ tecl function, changes in morphology, etc).
In ~ lition to using sequences constructed to hlL.,ll UIJt lldllsc,;~Lion of the open reading frarne, moflific~tions of gene cA~rcssion can be obtained by clesigning ~nticrnce sequences to intron regions, promoterlçnh~nrer ele nrntc, or even to trans-acting regulatory genes. Similarly, inhibition can be achieved using Hogeboom base-pairing methodology, also known as "triple helix" b~e pairing.

VII Expression of CAPE
Expression of CAPE is accomplished by subcloning the cDNAs into al~pro~ul;ate cA~les~ion vectors and lldn~rt;c~ g the vectors into ap~,ol,l;ate cAl~les~ion hosts. In this particular case, the cloning vector used in the generation of the full length clone also provides for cA~iession ofthe included cape sequence in E. coli. Up~llc~ll ofthe cloning site, this vector col~ s a promoter for 13-galactosidase, followed by sequence co.~ g the amino-termin~l Met and the ~ul sc4uent 7 residues of ~-galactosidase. Immediately following these eight W O 9~'4'922 PCT~US96/10179 residues is an PnginPPred bacteriophage promoter useful for artificial rriming and lldllscl;lJlion and for providing a number of unique endonnclP~ee restriction sites for cloning.Induction of the isolated, transfected bacterial strain with IPTG using standard methods will produce a fusion protein corresponding to the first seven residues of 13-galactosidase, about 5 15 residues of "linker", and the peptide encoded within the cDNA. Since cDNA clone inserts are g~ne.dled by an esePnti~lly random process, there is one chance in three that the included cDNA
will lie in the correct frame for proper translation. If the cDNA is not in the proper reading frame, it is obtained by deletion or insertion of the a~,plopl;ate number of bases by methods known to those of skill in the art including in vitro lllulagene~is, digestion with exonllcle~ee III
10 or mung bean nncle~ce, or oligonucleotide linker inclusion.
The cape cDNA is ehllttlPd into other vectors known to be useful for tA~ ,;,sion of protein in specific hosts. Oligonucleotide ~llplilllclsc~l~l;.;..;..g cloning sites as well as a segmPnt of DNA snfficient to hybridize to stretches at both ends of the target cDNA (25 bases) are synth~si7~d rh~mi~ lly by standard metho~e These primers can then be used to amplify the 15 desired gene segmpnte by PCR. The reslllting new gene seg...~ c can be ~ligP,sted with a~,up~;ate restriction el~yll,es under ~ dard conditions and isolated by gel electrophoresis.
Alternately, similar gene se~ le can be produced by digesting the cDNA with a~ro~ te restriction e.~yllles and filling in the mieeing gene segmPnte with chemically synthesi~ed oligonucleotides. Seg...l .~ ofthe coding sequence from more than one gene are ligated together and cloned in ~ypl'u~Jl;ate vectors to optimize ~A~ ion of recombinant sequence.Suitable ~A~I~;.sion hosts for such molecules include but are not limited to m~mm~ n cells such as Chinese Hamster Ovary (CHO) and human 293 cells, insect cells such as Sf9 cells, yeast cells such as Sacch~ lyces cerevisiae. and b~tPri~ such as E. coli. For each of these cell systems, a useful t;A~ ;.sion vector cont~ine an origin of replication to allow propagation in bacteria and a select~hle marker such as the B-l~e~ e antibiotic re~ e gene to allow selection in b~tPri~ In addition, the vectors may include a second selectable marker such as the neomycin phosphu~ r~,~se gene to allow selection in transfected eukaryotic host cells.
Vectors for use in eukaryotic ~AI"~ssion hosts may require RNA processing elements such as 3' polyadenylation sequences if such are not part of the cDNA of interest.
Additionally, the vector may contain promoters or enh~ncers which increase gene ~A~,ession. Such promoters are host specific and include MMTV, SV40, or metallothionine promoters for CHO cells; trp, lac, tac or T7 promoters for bacterial hosts, or alpha factor, alcohol WO 9f '4û~22 PCT/US96/10179 oxidase or PGH promoters for yeast. T~ scl~lJtion enh~nc~rs, such as the rous s~u~;ullla virus (RSV) enh~nr~., are used in m~mm~ n host cells. Once homogeneous cultures of recombinant cells are obtained through standard culture methods, large quantities of recombinantly produced CAPE is recovered from the conditioned medium and analyzed using chlo...atographic methods 5 known in the art.

VIII Isolation of Recombinant CAPE
CAPE is ~A~ ssed as a chimeric protein with one or more additional polypeptide domains added to the CAPE arnino acids to f~ilit~te protein purification. Such purification 10 facilit~ting domains include, but are not limited to, metal çh~l~ting peptides such as histi~line-LIyl~toph~ mo~llllPs that allow purification on immobilized metals, protein A domains that allow purification on immobilized immlln-)globulin, and the domain utilized in the FLAGS
extension/affinity purification system (T..l,..l.ll~,~ Corp., Seattle WA). The inclusion of a cleavable linker sequence such as Factor XA or enterokinase (Invitrogen) between the purification domain and the cape sequence is used to facilitate ~AI..c~ion of CAPE.

IX Diagnostic Test Using CAPE Specific Antibodies Particular CAPE antibodies are used for the ~ gn()sic of prepathologic conditions, and chronic or acute tli~ c.oc which are char~ctçri7~ by di~.ences in the amount or distribution of 20 CAPE. CAPE has been found to be ~AI~.essed in brain and hypoth~l~mllc cDNA libraries and thus is used to detect conditions that damage the hypoth~l~mnc Di~g~nstic tests for CAPE include m~tho~ tili7ing the antibody and a label to detect CAPE in human body fluids, tissues or extracts of such tissues. The polypeptides and antibodies of the present invention are used with or without modification. Frequently, the polypeptides and 25 antibodies will be labeled by joining them, either covalently or noncovalently, with a substance which provides for a ~içtect~hle signal. A wide variety of labels and conjugation techniques are known and have been reported extensively in both the scientific and patent iil~laLul~. Suitable labels include radionuclides, enzymes, sub~l~ales, cofactors, inhibitors, fluo.escc;..l agents, chemil--rninescent agents, chromogenic agents, m~gn~tic particles and the like. Patents tçaching the use of such labels include US Patent Nos. 3,817,837; 3,850,752; 3,939,350; 3,996,345;
4,277,437; 4,275,149; and 4,366,241. Also, recombinant immnnoglobulins may be produced as shown in US Patent No. 4,816,567, h~cOl~Olaled herein by ~e~l~..ce.

W O 9~'~0372 PCT~US96/10179 A variety of protocols for ~cllring soluble or membrane-bound CAPE, using eitherpolyclonal or monoclonal antibodies specific for the ~ ecli~re protein are known in the art.
Examples include enzyme-linked immlmosoll,elll ~say (ELISA), radioimml~no~cc~y (RIA) and fluolescenl activated cell sorting (FACS). A two-site monoclonal-b~ed immnno~cc~y ~Itili7ing monoclonal antibodies reactive to two non-hll~ ;llg epitopes on CAPE is ~ lled, but a colllp.,liLi~e binding ~say may be employed. These ~says are described, among other places, in Maddox, DE et al (1983, J Exp Med 158:1211).

X Purification of Native CAPE Using Specific Antiho~i~s o Native or recombinant CAPE is purified by immllnt~ffinity chronl~Lography using antibodies specific for CAPE. In general, an immlmn~ffinity column is constructed by covalently coupling the anti-CAPE antibody to an activated cl~l~olllalographic resin.
Polyclonal i.. ~ oglobulins are prel)al~d from ;.. ----~ sera either by pl~;cipiL~Lion with ammonium sulfate or by purification on immobilized Protein A (Ph~rm~ LKB Biotechnology, Pisc~t~w~, NJ). Simmil~rly~ monoclonal antibodies are prepared from mouse ~cites fluid by ammonium sulfate p~ iL~Lion or clllolfialography on immobilized Protein A. Partially purified immlln()globulin is covalently ~ rl~pd to a chromatographic resin such ~ CnBr-activated Sel)h~ose (Ph~rm~ LKB Biotechnology). The antibody is coupled to the resin, the resin is blocked, and the derivative resin is w~hed according to the m~mlf~tllrer's instructions.
Such immlln-l~ffinity columns are utilized in the purification of CAPE by pl~p~;llg a fraction from cells coll~ ;llg CAPE in a soluble form. This ~l~,p~Lion is derived by solllbili7~tion of the whole cell or of a subcellular fraction obtained via di~rellLial centrifugation by the addition of d ;;hl~,~,llt or by other methods well known in the art. Alternatively, soluble CAPE co.-l;.;..;..g a signal seqllence is secreted in useful quantity into the medium in in which the 25 cells are grown.
A soluble CAPE-co..l~ g ple~alalion is p~sed over the immnno~ffinity column, andthe column is w~hed under conditions that allow the l,ref~l~.ltial absoll,~lcc of serpin (eg, high ionic strength buffers in the presence of dt;l~,.gellL). Then, the column is eluted under conditions that disrupt antibody/CAPE binding (e.g., a buffer of pH 2-3 or a high concentration of a 30 chaotrope such ~ urea or thiocyanate ion), and CAPE is collected.

XI CAPE Activity W O g-'4~22 PCT~US96/10179 The activity of purified or t;~plc;,sed CAPE in plolease inhibition is tested by mixing a known quantity of the enzyme with a potential substrate ylotease such as chymotrypsin and a purified protein which chymotrypsin usually cleaves. The ability of a given amount of CAPE to inhibit chymotrypsin is assayed by FPLC of the protein fr~gm~nt~ produced under a given set of 5 conditions in a specific period of time.
In another method to test CAPE activity as a plotease inhibitor, a sample of the reaction materials are run on a non~ A~ hlg gel which s~ dLes the protease inhibitor CU111IJICA~
protease, inhibitor, protein sub~lldle and protein fr~gm~nt~ as dirr~le.lt si~d peptides.
The activity of purified or c~plc~sed CAPE in small molecule binding is tested by 10 incub~ting CAPE with various small molecules, preferably those derived from the hypoth~l~mnc or those that affect hypoth~l~mllc function, in radiolabeled form. After allowing a suitable time for binding, CAPE-bound small molecules may be separated from free small molecules by FPLC, and the binding affinity of CAPE for dirr~,. n~ small molecules is dt;t~.. ..;nP~

15 XII Rational Drug Design The goal of rational drug design is to produce structural analogs of biologically active polypeptides of interest or of small molecules with which they interact, eg, inhibitors, agonists, antagonists, etc. Any of these examples are used to fashion drugs which are more active or stable forms of the polypeptide or which ~..h~lre or hllclrt:lc with the function of a polypeptide in vivo (Hodgson J (1991) Bio/Technology 9:19-21, hlcoll~olaled herein by lcr~.cnce).
In one approach, the three-~im~n~ional structure of a protein of interest, or of a protein-inhibitor complex, is dett?nnin~d by x-ray crystallography, by collllJuL~,l modeling or, most typically, by a comhin~tion of the two approaches. Both the shape and charges of the polypeptide is ascertained to elucidate the structure and to d~ i n~ active site(s) of the molecule. Useful i~ llation regal.lillg the ~Llu~;lu~e of a polypeptide may be gained by modeling based on the structure of homologous proteins. In both cases, relevant structural infonn~tion is used to design analogous serpin-like molecules, to identify efficient inhibitors, or to identify small molecules that may bind serpins. Useful examples of rational drug design may include molecules which have improved activity or stability as shown by Braxton S and Wells JA (1992 Biochto~i~try 31 :7796-7801) or which act as inhibitors, agonists, or antagonists of native peptides as shown by Athauda SB et al (1993 J Biochem 113:742-746), incol~oldled herein by lefelellce.

W O 96/~0922 PCT~US96/10179 It is also possible to isolate a target-specific antibody, selected by functional assay, as described above, and then to solve its crystal structure. This apploach, in principle, yields a ph~ core upon which subsequent drug design can be based. It is possible to bypass protein crystallography altogether by genc,dlillg anti-idiotypic antibodies (anti-ids) to a functional, ph~n~cologically active antibody. As a mirror image of a mirror image, the binding site of the anti-ids is expected to be an analog of the original receptor. The anti-id is then used to identify and isolate peptides from banks of chemically or biologically produced peptides. The isolated peptides would then act as the ph~....~colc.
By virtue of the present invention, sufficient amount of polypeptide is made available to 10 pc,r."lll such analytical studies as X-ray crystallography. In addition, knowledge of the CAPE
amino acid sequence provided herein will provide guidance to those employing con~ L~I
modeling techniques in place of or in addition to x-ray crystallography.
All publications and patents mentioned in the above speçific~tion are herein incol~uuldlcd by reference. The folegohlg written specification is considered to be sufficient to enable one skilled in the art to practice the invention. Indeed, various modifications of the above described modes for ca~Tying out the invention which are readily a~ to those skilled in the field of molecular biology or related fields are intt?n~lPd to be within the scope of the following claims.

CA 02223086 l997-l2-02 W O 9f'~0322 PCTAJS96/10179 SUB~ ul~ S~YU~N~ LISTING
(1) GENERAL INFORMATION
(i) APPLICANT: Incyte Pharmaceuticals, Inc.
~ii) TITLE OF THE lNv~NllON: NOVEL SERPIN DERIVED FROM
HUMAN HYPOTHALAMUS
~iii) NUMBER OF ~U~N-~S: 5 (iv) CORRESPON~N-~ ADDRESS:
(A) ADDRESSEE: Incyte Pharmaceuticals, Inc.
(B) STREET: 3174 Porter Drive (C) CITY: Palo Alto (D) STATE: CA
(E) COUNl~Y: US
(F) ZIP: 94304 (v) COMPUTER READABLE FORM:
(A) MEDIUM TYPE: Diskette (B) COMPUTER: IBM Compatible (C) OPERATING SYSTEM: DOS
(D) SOFTWARE: FastSEQ Version 1.5 (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER: To Be Assigned (B) FILING DATE: Filed Herewith (vii) PRIOR APPLICATION DATA:
(A) APPLICATION NUMBER: US 08/487,823 (B) FILING DATE: 7-JUN-1995 (viii) AllORN~Y/AGENT INFORMATION:
(A) NAME: Luther, Barbara J.
(B) REGISTRATION NUMBER: 33,954 (C) REFERENCE/DOCKET NUMBER: PF-0039 PCT
(ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: 415-855-0555 (B) TELEFAX: 415-852-0195 (2) INFORMATION FOR SEQ ID NO:1:
(i) S~U~N-~ CHARACTERISTICS:
(A) LENGTH: 1558 base pairs (B) TYPE: nucleic acid (C) STR~N~ S: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA
(vii) IMMEDIATE SOURCE:
(A) LIBRARY: Hypothalamus (B) CLONE: 84476 (xi) ~UU~:N-~ DESCRIPTION: SEQ ID NO:1:

CA 02223086 l997-l2-02 W O 9''~922 PCTAJS96/10179 GTTTGAAACT GTTACAATAT GG~ C~lG GGA~ ll-GCl~l TCTGCAGAGT 120 ATGGCTACAG GGGCCACTTT CCCTGAGGAA GCCATTGTTG A~ll~L~AGT GAATATGTAT 180 AAl~l~.~A GAGCCACTGG TGAAGATGAA AATATTCTCT l~l~lC~ATT GAGTATTGCT 240 TCAATGGGAT ATGACAGCCT AAAAAATGGT GAAGAATTTT ~~ aAA GGA~ ~A 360 AACATGGTAA CTGCTAAAGA GAGCCAATAT GTGATGAAAA TTGCCAATTC ~ l~lG 420 GTGGAGAATA ACACAAACAA ~ GAAA GAll.~lAT CCCCAAGGGA TTTTNATGCT 600 GCCACTTATC TGGCCCTCAT TAAlG~l~lC TATTTCAAGG GGAACTGGAA GTCGCAGTTT 660 AGGCCTGAAA ATACTAGAAC ~lll.~l..C ACTAAAGATG ATGAAAGTGA AGTCCAAATT 720 CTGGTGCTGT CCAGACAGGA A~--C~-~-l GCTACTCTGG AGCCATTAGT CAAAGCACAG 900 ~lG~--~AAG AATGGGCAAA ~l~l~-~AAG AAGCAAAAAG TAGAAGTATA CCTGCCCAGG 960 TTCACAGTGG AACAGGA~AT TGATTTAAAA GA-~l-l-~A AGG~l~llGG AATAACTGAA 1020 ATTTTCATCA AGATCAAATT TGACAGCCTC TCTGATAATA AGGAGATTTT ~l~l~C~AAA 1080 GCAATTCACA A~l~U--C~-- AGAGGTTAAT GAAGAAGGCT CAGAACTCTC ~l~AGGA 1140 ATGATTCAAT TAGTAGGATG ~l~l~l~-AT CCTCAAGTTA ll~lCGACCA TCCATTTTTC 1200 111~1 lATCA GAAACAGGAG AACTGGTACA ATTCTATTCA TGGGACGAGT CATGCATCCT 1260 CAAGGAAAAC AGTAACTAAG CACATTATGT TTGCAACTGG TATATATTTA GGAl.l~l 1380 TTTACAGTAT ATCTTAAGAT AATATTTAAA ATAGTTCCAG ATAAAAACAA TATATGTA~A 1440 TTATAAGTAA ~ll~l~AAGG AATGTTATCA GTATTAAGCT AAl~lC~lG TTATGTCATT lS00 ~1~ L 1 ~ ~1~ ~ G-l~-l~--~ AAAATAAAAG TACCTATTGA AAAAAAAAAA AAAAAAAA 15S8 (2) INFORMATION FOR SEQ ID NO:2:
(i) ~Qu~ CHARACTERISTICS:
(A) LENGTH: 407 amino acids (B) TYPE: amino acid (C) STRANn~nNRCS single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) S~UU~N~ DESCRIPTION: SEQ ID NO:2:
Met Ala Phe Leu Gly Leu Phe Ser Leu Leu Val Leu Gln Ser Met Ala 1 5 10 lS
Thr Gly Ala Thr Phe Pro Glu Glu Ala Ile Val Asp Leu Ser Val Asn Met Tyr Asn Arg Leu Arg Ala Thr Gly Glu Asp Glu Asn Ile Leu Phe Ser Pro Leu Ser Ile Ala Leu Ala Met Gly Met Met Glu Leu Gly Ala Gln Gly Ser Thr Gln Lys Glu Ile Arg His Ser Met Gly Tyr Asp Ser Leu Lys Asn Gly Glu Glu Phe Ser Phe Leu Lys Glu Phe Ser Asn Met Val Thr Ala Lys Glu Ser Gln Tyr Val Met Lys Ile Ala Asn Ser Leu Phe Val Gln Asn Gly Phe His Val Asn Glu Glu Phe Leu Gln Met Met Lys Lys Tyr Phe Asn Ala Ala Val Asn His Val Asp Phe Ser Gln Asn W O 96/40922 PCT~US96/10179 Val Ala Val Ala Asn Tyr Ile Asn Lys Trp Val Glu Asn Asn Thr Asn Asn Leu val Lys Asp Leu Val Ser Pro Arg Asp Phe Xaa Ala Ala Thr Tyr Leu Ala Leu Ile Asn Ala Val Tyr Phe Lys Gly Asn Trp Lys Ser Gln Phe Arg Pro Glu Asn Thr Arg Thr Phe Ser Phe Thr Lys Asp Asp Glu Ser Glu Val Gln Ile Pro Met Met Tyr Gln Gln Gly Glu Phe Tyr Tyr Gly Glu Phe Ser Asp Gly Ser Asn Glu Ala Gly Gly Ile Tyr Gln Val Leu Glu Ile Pro Tyr Glu Gly Asp Glu Ile Ser Met Met Leu Val Leu Ser Arg Gln Glu Val Pro Leu Ala Thr Leu Glu Pro Leu Val Lys Ala Gln Leu Val Glu Glu Trp Ala Asn Ser Val Lys Lys Gln Lys Val Glu Val Tyr Leu Pro Arg Phe Thr Val Glu Gln Glu Ile Asp Leu Lys Asp Val Leu Lys Ala Leu Gly Ile Thr Glu Ile Phe Ile Lys Ile Lys Phe Asp Ser Leu Ser Asp Asn Lys Glu Ile Phe Leu Ser Lys Ala Ile His Lys Ser Phe Leu Glu Val Asn Glu Glu Gly Ser Glu Leu Ser Val Ser Gly Met Ile Gln Leu Val Gly Cys Cys Leu Tyr Pro Gln Val Ile Val Asp His Pro Phe Phe Phe Leu Ile Arg Asn Arg Arg Thr Gly Thr Ile Leu Phe Met Gly Arg Val Met His Pro Glu Thr Met Asn Thr Ser Gly His Asp Phe Glu Glu Leu (2) INFORMATION FOR SEQ ID NO:3:
(i) ~Uu~ CHARACTERISTICS:
(A) LENGTH: 382 amino acids (B) TYPE: amino acid (C) STR.P~ NI~ S: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) ~Y~O~ CAL: NO
(iv) ANTISENSE: NO
(v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE:
(xi) ~ u~N~ DESCRIPTION: SEQ ID NO:3:
Met Glu Asp Leu Cys Val Ala Asn Thr Leu Phe Ala Leu Asn Leu Phe Lys His Leu Ala Lys Ala Ser Pro Thr Gln Asn Leu Phe Leu Ser Pro Trp Ser Ile Ser Ser Thr Met Ala Met Val Tyr Met Gly Ser Arg Gly CA 02223086 l997-l2-02 W O 9f":C922 PCT~US96/10179 Ser Thr Glu Asp Gln Met Ala Lys Val Leu Gln Phe Asn Glu Val Gly Ala Ala Ala Asp Lys Ile His Ser Ser Phe Arg Ser Leu Ser Ser Ala Ile Asn Ala Ser Thr Gly Asn Tyr Leu Leu Glu Ser Val Asn Lys Leu Phe Gly Glu Lys Ser Ala Ser Phe Arg Glu Glu Tyr Ile Arg Leu Cys Gln Lys Tyr Tyr Ser Ser Glu Pro Gln Ala Val Asp Phe Leu Glu Cys Ala Glu Glu Ala Arg Lys Lys Ile Asn Ser Trp Val Lys Thr Gln Thr Lys Gly Lys Ile Pro Asn Leu Leu Pro Glu Gly Ser Val Asp Gly Asp Thr Arg Met Val Leu Val Asn Ala Val Tyr Phe Lys Gly Lys Trp Lys Thr Pro Phe Glu Lys Lys Leu Asn Gly Leu Tyr Pro Phe Arg Val Asn Ser Ala Gln Arg Thr Pro Val Gln Met Met Tyr Leu Arg Glu Lys Leu Asn Ile Gly Tyr Ile Glu Asp Leu Lys Ala Gln Ile Leu Glu Leu Pro Tyr Ala Gly Asp Val Ser Met Phe Leu Leu Leu Pro Asp Glu Ile Ala Asp Val Ser Thr Gly Leu Glu Leu Leu Glu Ser Glu Ile Thr Tyr Asp Lys Leu Asn Lys Trp Thr Ser Lys Asp Lys Met Ala Glu Asp Glu Val Glu Val Tyr Ile Pro Gln Phe Lys Leu Glu Glu His Tyr Glu Leu Arg Ser Ile Leu Arg Ser Met Gly Met Glu Asp Ala Phe Asn Lys Gly Arg Ala Asn Phe Ser Gly Met Ser Glu Arg Asn Asp Leu Phe Leu Ser Glu Val Phe His Gln Ala Met Val Asp Val Asn Glu Glu Gly Thr Glu Ala Ala Ala Gly Thr Gly Gly Val Met Thr Gly Arg Thr Gly His Gly Gly Pro Gln Phe Val Ala ASp His Pro Phe Leu Phe Leu Ile Met His Lys Ile Thr Asn Cys Ile Leu Phe Phe Gly Arg Phe Ser Ser Pro (2) INFORMATION FOR SEQ ID NO:4:
(i) ~U~N~ CHARACTERISTICS:
(A) LENGTH: 420 amino acids (B) TYPE: amino acid (C) STRAN~N~SS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO
(iv) ANTISENSE: NO
(v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE:

CA 02223086 l997-l2-02 W O 9~/1C322 PCTAJS96/10179 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:
Met Ala Phe Leu Gly Leu Phe Ser Leu Leu Val Leu Glu Ser Leu Ala l 5 10 15 Val Gly Ala Thr Leu Pro Glu Glu Ala Ile Val Asp Leu Ala Val Asn Leu Phe Asn His Leu Ala Ala Ala Gly Glu Thr Glu Asn Leu Leu Leu Ser Pro Leu Ser Ile Ala Leu Ala Met Gly Met Val Glu Leu Gly Ala Gln Gly Ser Thr Glu Asp Glu Ile Ala Lys Val Leu Gly Phe Asp Ser Val Gly Ala Gly Ala Asp Lys Ile His Ser Ser Leu Lys Ser Leu Ser Ser Ala Val Thr Ala Ser Thr Gly Asn Tyr Val Leu Glu Ile Ala Asn Ser Leu Phe Gly Glu Asn Gly Ala Ser Val Asn Glu Glu Phe Leu Gln Leu Cys Gln Lys Tyr Phe Ser Ala Ala Val Asn Ala Val Asp Phe Leu Glu Asn Ala Ala Val Ala Arg Asn Lys Ile Asn Ser Trp Val Glu Thr Asn Thr Asn Gly Leu Val Lys Asp Leu Val Ser Glu Gly Ser Val Xaa Gly Ala Thr Arg Leu Ala Leu Val Asn Ala Val Tyr Phe Lys Gly Asn Trp Lys Ser Gln Phe Glu Lys Glu Leu Thr Gly Leu Phe Ser Phe Thr Val Asp Ser Ala Ser Glu Val Gln Val Gln Met Met Tyr Leu Gln Gly Glu Leu Asn Ile Gly Glu Phe Ile Asp Gly Leu Asn Ala Ala Gly Gly Ile Tyr Gln Val Leu Glu Leu Pro Tyr Ala Gly Asp Glu Val Ser Met Phe Leu Val Leu Ser Asp Glu Ile Ala Asp Val Ala Thr Gly Leu Glu Leu Leu Glu Ser Leu Val Thr Ala Asp Leu Val Glu Glu Trp Ala Ser Ser Val Lys Lys Ala Glu Asp Glu Val Glu Val Tyr Leu Pro Gln Phe Thr Val Glu Glu Glu Ile Asp Leu Lys Ser Val Leu Lys Ala Leu Gly Ile Thr Asp Ala Phe Ile Lys Gly Lys Ala Asn Phe Ser Gly Leu Ser Asp Asn Asn Asp Leu Phe Leu Ser Glu Ala Ile His Gln Ala Phe Val Asp Val Asn Glu Glu Gly Ser Glu Ala Ala Ala Gly Gly Gly Gly Val Leu Val Gly Arg Thr Gly His Gly Gly Pro Gln Val Val Ala Asp His Pro Phe Leu Phe Leu Ile Arg Asn Lys Ile Thr Gly Thr Ile Leu Phe Phe Gly Arg Val Met His Pro Glu Thr Met Asn Thr Ser Gly His Asp Phe Ser Ser Leu CA 02223086 l997-l2-02 W O 96/40922 PCTtUS96tlO179 (2) INFORMATION FOR SEQ ID NO:5:
(i) S~yU~N~ CHARACTERISTICS:
(A) LENGTH: 406 amino acids (B) TYPE: amino acid (C) STR~Nn~nN~s single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide (iii) HYPOTHETICAL: NO
(iv) ANTISENSE: NO
(v) FRAGMENT TYPE: N-terminal (vi) ORIGINAL SOURCE:
(Xi ) ~U~N~'~ DESCRIPTION: SEQ ID NO:5:
Met Ala Thr Gly Ala Val Phe Pro Glu Glu Ala Ile Val Leu Leu Ala Val Asn Leu Phe Asn His Leu Ala Ala Ala Gly Glu Thr Glu Asn Leu Leu Leu Ser Pro Leu Ser Ile Ala Leu Ala Met Gly Met Val Glu Leu Gly Ala Gln Gly Ser Thr Glu Asp Glu Ile Ala Lys Val Leu Gly Phe Asp Ser Val Gly Ala Gly Ala Asp Lys Ile His Ser Ser Leu Lys Ser Leu Ser Ser Ala Val Thr Ala Ser Thr Gly Asn Tyr Val Leu Glu Ile Ala Asn Ser Leu Phe Gly Glu Asn Gly Ala Ser Val Asn Glu Glu Phe Leu Gln Leu Cys Gln Lys Tyr Phe Ser Ala Ala Val Asn Ala Val Asp Phe Leu Glu Asn Ala Ala Val Ala Arg Asn Lys Ile Asn Ser Trp Val Glu Thr Asn Thr Asn Gly Leu Val Lys Asp Leu Val Ser Glu Gly Ser Val Xaa Gly Ala Thr Arg Leu Ala Leu Val Asn Ala Val Tyr Phe Lys Gly Asn Trp Lys Ser Gln Phe Glu Lys Glu Leu Thr Gly Leu Phe Ser Phe Thr Val Asp Ser Ala Ser Glu Val Gln Val Gln Met Met Tyr Leu Gln Gly Glu Leu Asn Ile Gly Glu Phe Ile Asp Gly Leu Asn Ala Ala Gly Gly Ile Tyr Gln Val Leu Glu Leu Pro Tyr Ala Gly Asp Glu Val Ser Met Phe Leu Val Leu Ser Asp Glu Ile Ala Asp Val Ala Thr Gly Leu Glu Leu Leu Glu Ser Leu Val Thr Ala Asp Leu Val Glu Glu Trp Ala Ser Ser Val Lys Lys Ala Glu Asp Glu Val Glu Val Tyr Leu Pro Gln Phe Thr Val Glu Glu Glu Ile Asp Leu Lys Ser Val Leu Lys Ala Leu Gly Ile Thr Asp Ala Phe Ile Lys Gly Lys Ala Asn Phe Ser Gly W O 9-'4~322 PCTAJS96/10179 Leu Ser Asp Asn Asn Asp Leu Phe Leu Ser Glu Ala Ile His Gln Ala Phe Val Asp Val Asn Glu Glu Gly Ser Glu Ala Ala Ala Gly Gly Gly Gly Val Leu Val Gly Arg Thr Gly His Gly Gly Pro Gln Val Val Ala Asp His Pro Phe Leu Phe Leu Ile Arg Asn Lys Ile Thr Gly Thr Ile Leu Phe Phe Gly Arg Val Met His Pro Glu Thr Met Asn Thr Ser Gly His Asp Phe Ser Ser Leu

Claims (14)

1. A purified polynucleotide comprising a nucleic acid sequence encoding the polypeptide having the sequence as depicted in SEQ ID NO:2, or its complement.

2. The polynucleotide of Claim 1 wherein the nucleic acid sequence consists of SEQ ID NO:1.

3. An expression vector comprising the polynucleotide of Claim 2.

4. A host cell comprising the expression vector of Claim 3.

5. A nucleic acid probe comprising a non-conserved fragment of the polynucleotide of Claim 2.

6. An antisense molecule comprising a polynucleotide sequence complementary to at least a portion of the polynucleotide of Claim 2.

7. A method for producing a polypeptide comprising the sequence as depicted in SEQ ID NO:2, said method comprising:
a) culturing the host cells of Claim 4 under conditions suitable for the expression of the polypeptide, and b) recovering said polypeptide from the cell culture.

8. A purified CAPE molecule having the amino acid sequence as depicted in SEQ ID NO:2.

9. An antibody specific for the purified polypeptide of Claim 8.

10. An diagnostic composition for the detection of nucleic acid sequences encoding CAPE
comprising the nucleic acid probe of Claim 5.

11. A diagnostic test for the detection of nucleotide sequences encoding CAPE in a biological sample, comprising the steps of:

a) combining the biological sample with a polynucleotide which comprises the nucleotide sequence of SEQ ID NO: 1 or a fragment thereof, wherein said fragment is derived from a non-conserved region of said nucleotide, under conditions suitable for the formation of a nucleic acid hybridization complex between the nucleic acid sequence of SEQ ID NO: 1 and a complementary nucleic acid sequence in said sample;

b) detecting said hybridization complex; and c) comparing the amount of said hybridization complex with a standard wherein the presence of an abnormal level of said hybridization complex correlates positively with a condition associated with inflammation.

12. The diagnostic test of Claim 11 wherein the polynucleotide is labeled with a reporter molecule and the hybridization complex is detected by measuring said reporter molecule.

13. A diagnostic test for the detection of nucleotide sequences encoding CAPE in a biological sample, comprising the steps of:

a)combining the biological sample with polymerase chain reaction primers under conditions suitable for nucleic acid amplification, wherein said primers comprise fragments from non-conserved regions of the nucleotide sequence of SEQ ID NO: 1;
b) detecting amplified nucleotide sequences; and c) comparing the amount of amplified nucleotide sequences in said biological sample with a standard thereby determining whether the amount of said nucleotide sequence varies from said standard, wherein the presence of an abnormal level of said nucleotide sequence correlates positively with a condition associated with inflammation.

14. A method of screening a plurality of compounds for specific binding affinity with the polypeptide of Claim 8 or any portion thereof, comprising the steps of:

a) providing a plurality of compounds;

b) combining CAPE with each of a plurality of compounds for a time sufficient to allow binding under suitable conditions; and c) detecting binding of CAPE to each of the plurality of compounds, thereby identifying the compounds which specifically bind CAPE.