CA2211359A1 - Heterorhabditis bacteriophora insecticide compositions and related processes - Google Patents
Heterorhabditis bacteriophora insecticide compositions and related processesInfo
- Publication number
- CA2211359A1 CA2211359A1 CA002211359A CA2211359A CA2211359A1 CA 2211359 A1 CA2211359 A1 CA 2211359A1 CA 002211359 A CA002211359 A CA 002211359A CA 2211359 A CA2211359 A CA 2211359A CA 2211359 A1 CA2211359 A1 CA 2211359A1
- Authority
- CA
- Canada
- Prior art keywords
- insecticidal
- soil
- nematodes
- nematode
- insects
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 12
- 241001523412 Heterorhabditis bacteriophora Species 0.000 title claims abstract description 11
- 239000002917 insecticide Substances 0.000 title description 13
- 241000244206 Nematoda Species 0.000 claims abstract description 39
- 241000238631 Hexapoda Species 0.000 claims abstract description 23
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 20
- 230000000967 entomopathogenic effect Effects 0.000 claims abstract description 9
- 239000002689 soil Substances 0.000 claims abstract description 8
- 230000000366 juvenile effect Effects 0.000 claims abstract description 6
- 241000254099 Melolontha melolontha Species 0.000 claims description 2
- 230000035558 fertility Effects 0.000 description 6
- 241000255896 Galleria mellonella Species 0.000 description 5
- 241000607479 Yersinia pestis Species 0.000 description 5
- 230000001524 infective effect Effects 0.000 description 5
- 241000255890 Galleria Species 0.000 description 3
- 241001523406 Heterorhabditis Species 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 206010011906 Death Diseases 0.000 description 1
- 241001414890 Delia Species 0.000 description 1
- 241001414892 Delia radicum Species 0.000 description 1
- 241000489975 Diabrotica Species 0.000 description 1
- 241001243087 Gryllotalpidae Species 0.000 description 1
- 241001465746 Heterorhabditidae Species 0.000 description 1
- 208000000291 Nematode infections Diseases 0.000 description 1
- 241001646398 Pseudomonas chlororaphis Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000010410 calcium alginate Nutrition 0.000 description 1
- 229960002681 calcium alginate Drugs 0.000 description 1
- 239000000648 calcium alginate Substances 0.000 description 1
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000009428 plumbing Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000005641 tunneling Effects 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
- A01N63/12—Nematodes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Animal Behavior & Ethology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Provided is an insecticidal composition comprising an effective amount of a juvenile infective-stage of insecticidal entomopathogenic Heterorhabditis bacteriophora hybrid strain Hb (NJ), or mutants or variants thereof and a biocompatible carrier therefor. The insecticidal compositions provided have improved insecticidal activity compared to known insecticidal compositions containing a known H. bacteriophora nematode HBHP88. Also disclosed is a process for reducing the population of soil-inhabiting insects in soil by applying to a soil locus infected with such insects the above-described insecticidal composition. Additionally, the Hb (NJ) hybrid nematode strain is provided.
Description
CA 022113~9 1997-07-24 Heterorhabditis Bact~riophora Insecticide Compositions and Related Processes FIELD OF INVENTION
This invention relates to novel insecticide compositions comprising infective-stage heterorhabditid hybrid nematodes with enhanced insecticidal effectiveness, novel nematodes having said characteristics, and process for reducing soil-inhabiting insect pest populations by applying an effective amount of said insecticide composition to the infected locus.
BACKGROUND O~ INVENTION
Insecticide compositions having as an ingredient a biological insecticide or entomopathogenic nematodes offer an attractive field of biological control agents for use against soil-inhabiting insect pests. They offer many important advantages over available chemical insecticides. Advantages of insecticide nematodes include high virulence, broad host range, safety, exemption from government registration and ease of mass production.
Nematodes of the family Heterorhabditidae form a unique symbiotic association with Photorhabd2/s bacteria. They are pathogenic to a diverse group of insects. Heterorhabditids are hermaphroditic. In general, infective juveniles penetrate into the hemocoel of their host insects such as white grubs,usually through the gut or spiracles. Heterorhabditids have a dorsal tooth that might also facilitate penetration in other areas (e.g., intersegmental membranes). Bacteria from the digestive system of the nematodes are released within the host, and the host dies from septicemia, typically within 48 h. The nematodes feed, develop, mate and reproduce within the host cadaver, and, often after multiple generations, infective juveniles are again produced, emerge, and seek new host insects.
W O 96/23871 PCT~US96/02603 It is desired to provide insecticide compositions comprising insecticide nçm~todes which provide improved effectiveness against soil-inhabiting insect pests, novel nematodes providing the improved effectiveness, and the process for controlling soil-inhabiting insect pests by use of such improved insecticidenematodes, and process and device for producing said improved insecticide 1 5 nematodes.
SUMMARY OF INVENTION
Provided by this invention are novel insecticide compositions and an improved process for controlling soil-inhabiting insect pests by applying said novel insecticide composition having an effective amount of infective-stage entomopathogenic nematodes of this invention to the infested locus. The insecticidal compositions of this invention comprise an effective amount of a juvenile infective-stage of an insecticidal entomopathogenic nematode having insecticidal properties of Heterorhabdi~is bacleriophora hybrid strain HB (NJ) and effective variants, mutants or hybrids thereof and a biocompatible carrier therefor. Also, provided by this invention are the novel entomopathogenic nematodes of the insecticidal compositions of this invention, which have high effectiveness in controlling soil-inhabiting insects including an increased pathogenicity to diverse insect hosts and an enhanced fecundity.
CA 022113~9 1997-07-24 DETAILED DESCRIPTION O~ THE INVENTION
The hybrid strain of Heterorhabditis bacteriophora HB (NJ) was attained by hybridizing a large series of wild-type isolates of H. bacteriophoraobtained from soil samples collected from four dirrerent habitats. The habitats were turf, turf with scattered trees, weedy field and weedy field with scatteredtrees. The habitats represented many collection sites.
The enhanced properties of the hybrid strain include enhanced patho-genicity or infectivity to diverse insect hosts and increased fecundity.
Infectivity is deterrnined by the following test. The standard test of infectivity used is to apply 50 infective juveniles to the top of a sand column (5 cm diameter x 5 cm high) with a target insect (the wax moth, Galleria mellonella) at the bottom. The test is typically run at 25~C and the sand contains 10% moisture by weight. The nematodes are allowed 24 h to find the target insect and at that point the insect is removed, rinsed in distilled water and incubated for a further 24 h at 25~C to allow the nematodes time to develop in the host. The host is then dissected and the number of nematodes that successfully established in the host is counted. In two tests of 20 replicates each, HB (NJ) had a mean infectivity rate of 10.45 and 11.1 nematodes per host (SE = 1.053 and 1.095 respectively) with all hosts having been infected and killed during the experiment. In comparable tests, the HB (NJ) strain showed results much superior to results of two known H. bacteriophora strains (HB~C
and HBHP88). They killed only 50% and 70% of the hosts, respectively, and had mean infectivity rates of only 0.08 and 1.20 nematodes per host, respectively. Infectivity rates against other insects indicate similar superiority of the HB (NJ) strain.
WO 96/23871 CA o 2 2 1 13 ~ 9 19 9 7 - o 7 - 2 4 PCT/US96102603 Fecundity is determined by the following test. The standard test of fecundity is to quantify the number of new infective juveniles produced from single hosts (Galleria nle110nella) of a standard weight (0.24-0.26 gms) and with a standard inoculation of infective juveniles (n = 20) at 25~C. For 30 d replicates with HB (NJ), a mean fecundity level of 383,046.7 (SE = 15212.9) was obtained. Indications are that the fecundity of HB (NJ) is superior.
The Heterorhabditis bac1eriophora hybrid strain HB (NJ) provided by this invention has a deposit number of ATCC
The insecticidal compositions can be in the folm of an aqueous spray composition, in the form of gel pellets or granules made using calcium alginate or other suitable carriers, in the form of a solid sheet, and the like.
The carriers, which immobilize the nematodes or partially desiccate them, reduce their metabolism and improve their tolerance to temperature extremes. Nematodes are also commercially available on a moist substrate such as sponge, vermiculite, and peat, although extended storage at room tem-perature is generally poor because nematode viability declines rapidly. The Heterorhabditid compositions of this invention are suitably stored at 10~C-15~C and the org~nism.c should be maintained in the presence of oxygen.
Nematodes can be applied with common agrichemical equipment including small pressurized sprayers, mist blowers, electrostatic sprayers, fan sprayers, and helicopters. Entomopathogenic nematodes can withstand appli-cation pressures of 300 lb/in' and can be delivered with all common nozzle type sprayers (e.g., "01" nozzles) with openings as small as 50 microns in diameter.
However, some types of pumping equipment produce a considerable amount of heat, and should the temperature in the sprayer plumbing rise above 32~C, the nematodes could be adversely affected. Fortunately, this rarely occurs and is CA 022113~9 1997-07-24 usually only a problem when the spray tank is almost empty and liffle water is available as a heat sink.
Field concentrations exceeding 2.5 billion nematodes/ha (l billion/acre) are usually applied to ensure that a sufficient nematode population will come incontact with the target insects to provide control. A high concentration is needed to overcome the negative impacts of the abiotic and biotic soil environ-ment. High nematode concentrations are also needed against certain insects which only remain in soil for a few days before tunneling into roots, such as cabbage maggots (D. radiC21M?), and against insects which are not highly sus-ceptible to nematode infection because of their small size and active movement.
For example, high nematode concenbrations are desirable against early imm~ re stages of mole crickets (Scap~erisc2~s spp.), root maggots (Delia spp.). and corn rootwor~ns (Diabrotica spp.) An extensive discussion of formulation and application applicable to providing the insecticidal compositions of this invention and the processes of this invention is found in the breatise Entomopathogenic Nematodes in Biologi-cal Control, editors Randy Gaugler, Ph.D. and Harry K. Kaya. Ph.D., Chapter 9. Forml~l~ion and Application Technology, by Ramon Georgis, CRC Press (Boca Raton, Ann Arbor, Boston), l990.
The Galleria mellonella recognized test for evaluating insecticidal activity of nematodes is carried out as follows~ n No. 1 filter paper is placed in 35 mm pebi dishes. A Galleria mellonella host is placed on the filter paper. In 500 microliters of water, lOO juvenile nematodes are suspended. The aqueous suspension is placed on the filter paper by wetting the filter paper in several locations. If the Galleria mellonella host has turned red at the end of the test period, this is a showing that the Galleria n7ellonella host is dead.
CA 022113~9 1997-07-24 In the Galleria mellonella bait method for isolating nematodes, the Galleria mellonel1a infected with Heterorhabditis bacferiophora normally die and turn a red color. The soil samples taken are 2 cm in diameter and lS cm in length. In providing the He~erorhabditis bacteriophora hybrid strain HB (NJ) of this invention, about lS0 Heterorhabditis bacteriophora isolates were utilized.
The crossbreeding used to provide the HB (NJ) strain involve conventional procedures.
Changes and modifications of the invention provided will be apparent to those skilled in the art and to the extent they are within the spirit of this invention, are intended to be within the scope thereof.
This invention relates to novel insecticide compositions comprising infective-stage heterorhabditid hybrid nematodes with enhanced insecticidal effectiveness, novel nematodes having said characteristics, and process for reducing soil-inhabiting insect pest populations by applying an effective amount of said insecticide composition to the infected locus.
BACKGROUND O~ INVENTION
Insecticide compositions having as an ingredient a biological insecticide or entomopathogenic nematodes offer an attractive field of biological control agents for use against soil-inhabiting insect pests. They offer many important advantages over available chemical insecticides. Advantages of insecticide nematodes include high virulence, broad host range, safety, exemption from government registration and ease of mass production.
Nematodes of the family Heterorhabditidae form a unique symbiotic association with Photorhabd2/s bacteria. They are pathogenic to a diverse group of insects. Heterorhabditids are hermaphroditic. In general, infective juveniles penetrate into the hemocoel of their host insects such as white grubs,usually through the gut or spiracles. Heterorhabditids have a dorsal tooth that might also facilitate penetration in other areas (e.g., intersegmental membranes). Bacteria from the digestive system of the nematodes are released within the host, and the host dies from septicemia, typically within 48 h. The nematodes feed, develop, mate and reproduce within the host cadaver, and, often after multiple generations, infective juveniles are again produced, emerge, and seek new host insects.
W O 96/23871 PCT~US96/02603 It is desired to provide insecticide compositions comprising insecticide nçm~todes which provide improved effectiveness against soil-inhabiting insect pests, novel nematodes providing the improved effectiveness, and the process for controlling soil-inhabiting insect pests by use of such improved insecticidenematodes, and process and device for producing said improved insecticide 1 5 nematodes.
SUMMARY OF INVENTION
Provided by this invention are novel insecticide compositions and an improved process for controlling soil-inhabiting insect pests by applying said novel insecticide composition having an effective amount of infective-stage entomopathogenic nematodes of this invention to the infested locus. The insecticidal compositions of this invention comprise an effective amount of a juvenile infective-stage of an insecticidal entomopathogenic nematode having insecticidal properties of Heterorhabdi~is bacleriophora hybrid strain HB (NJ) and effective variants, mutants or hybrids thereof and a biocompatible carrier therefor. Also, provided by this invention are the novel entomopathogenic nematodes of the insecticidal compositions of this invention, which have high effectiveness in controlling soil-inhabiting insects including an increased pathogenicity to diverse insect hosts and an enhanced fecundity.
CA 022113~9 1997-07-24 DETAILED DESCRIPTION O~ THE INVENTION
The hybrid strain of Heterorhabditis bacteriophora HB (NJ) was attained by hybridizing a large series of wild-type isolates of H. bacteriophoraobtained from soil samples collected from four dirrerent habitats. The habitats were turf, turf with scattered trees, weedy field and weedy field with scatteredtrees. The habitats represented many collection sites.
The enhanced properties of the hybrid strain include enhanced patho-genicity or infectivity to diverse insect hosts and increased fecundity.
Infectivity is deterrnined by the following test. The standard test of infectivity used is to apply 50 infective juveniles to the top of a sand column (5 cm diameter x 5 cm high) with a target insect (the wax moth, Galleria mellonella) at the bottom. The test is typically run at 25~C and the sand contains 10% moisture by weight. The nematodes are allowed 24 h to find the target insect and at that point the insect is removed, rinsed in distilled water and incubated for a further 24 h at 25~C to allow the nematodes time to develop in the host. The host is then dissected and the number of nematodes that successfully established in the host is counted. In two tests of 20 replicates each, HB (NJ) had a mean infectivity rate of 10.45 and 11.1 nematodes per host (SE = 1.053 and 1.095 respectively) with all hosts having been infected and killed during the experiment. In comparable tests, the HB (NJ) strain showed results much superior to results of two known H. bacteriophora strains (HB~C
and HBHP88). They killed only 50% and 70% of the hosts, respectively, and had mean infectivity rates of only 0.08 and 1.20 nematodes per host, respectively. Infectivity rates against other insects indicate similar superiority of the HB (NJ) strain.
WO 96/23871 CA o 2 2 1 13 ~ 9 19 9 7 - o 7 - 2 4 PCT/US96102603 Fecundity is determined by the following test. The standard test of fecundity is to quantify the number of new infective juveniles produced from single hosts (Galleria nle110nella) of a standard weight (0.24-0.26 gms) and with a standard inoculation of infective juveniles (n = 20) at 25~C. For 30 d replicates with HB (NJ), a mean fecundity level of 383,046.7 (SE = 15212.9) was obtained. Indications are that the fecundity of HB (NJ) is superior.
The Heterorhabditis bac1eriophora hybrid strain HB (NJ) provided by this invention has a deposit number of ATCC
The insecticidal compositions can be in the folm of an aqueous spray composition, in the form of gel pellets or granules made using calcium alginate or other suitable carriers, in the form of a solid sheet, and the like.
The carriers, which immobilize the nematodes or partially desiccate them, reduce their metabolism and improve their tolerance to temperature extremes. Nematodes are also commercially available on a moist substrate such as sponge, vermiculite, and peat, although extended storage at room tem-perature is generally poor because nematode viability declines rapidly. The Heterorhabditid compositions of this invention are suitably stored at 10~C-15~C and the org~nism.c should be maintained in the presence of oxygen.
Nematodes can be applied with common agrichemical equipment including small pressurized sprayers, mist blowers, electrostatic sprayers, fan sprayers, and helicopters. Entomopathogenic nematodes can withstand appli-cation pressures of 300 lb/in' and can be delivered with all common nozzle type sprayers (e.g., "01" nozzles) with openings as small as 50 microns in diameter.
However, some types of pumping equipment produce a considerable amount of heat, and should the temperature in the sprayer plumbing rise above 32~C, the nematodes could be adversely affected. Fortunately, this rarely occurs and is CA 022113~9 1997-07-24 usually only a problem when the spray tank is almost empty and liffle water is available as a heat sink.
Field concentrations exceeding 2.5 billion nematodes/ha (l billion/acre) are usually applied to ensure that a sufficient nematode population will come incontact with the target insects to provide control. A high concentration is needed to overcome the negative impacts of the abiotic and biotic soil environ-ment. High nematode concentrations are also needed against certain insects which only remain in soil for a few days before tunneling into roots, such as cabbage maggots (D. radiC21M?), and against insects which are not highly sus-ceptible to nematode infection because of their small size and active movement.
For example, high nematode concenbrations are desirable against early imm~ re stages of mole crickets (Scap~erisc2~s spp.), root maggots (Delia spp.). and corn rootwor~ns (Diabrotica spp.) An extensive discussion of formulation and application applicable to providing the insecticidal compositions of this invention and the processes of this invention is found in the breatise Entomopathogenic Nematodes in Biologi-cal Control, editors Randy Gaugler, Ph.D. and Harry K. Kaya. Ph.D., Chapter 9. Forml~l~ion and Application Technology, by Ramon Georgis, CRC Press (Boca Raton, Ann Arbor, Boston), l990.
The Galleria mellonella recognized test for evaluating insecticidal activity of nematodes is carried out as follows~ n No. 1 filter paper is placed in 35 mm pebi dishes. A Galleria mellonella host is placed on the filter paper. In 500 microliters of water, lOO juvenile nematodes are suspended. The aqueous suspension is placed on the filter paper by wetting the filter paper in several locations. If the Galleria mellonella host has turned red at the end of the test period, this is a showing that the Galleria n7ellonella host is dead.
CA 022113~9 1997-07-24 In the Galleria mellonella bait method for isolating nematodes, the Galleria mellonel1a infected with Heterorhabditis bacferiophora normally die and turn a red color. The soil samples taken are 2 cm in diameter and lS cm in length. In providing the He~erorhabditis bacteriophora hybrid strain HB (NJ) of this invention, about lS0 Heterorhabditis bacteriophora isolates were utilized.
The crossbreeding used to provide the HB (NJ) strain involve conventional procedures.
Changes and modifications of the invention provided will be apparent to those skilled in the art and to the extent they are within the spirit of this invention, are intended to be within the scope thereof.
Claims (6)
1. An insecticidal composition useful in reducing the population of one or more soil-inhabiting insects comprising an effective amount of a juvenile infective-stage of an insecticidal entomopathogenic nematode having insecticidal properties of Heterorhabditis bacteriophora hybrid strain Hb(NJ) or effective variants or mutants thereof and a biocompatible carrier therefor.
2. A composition of Claim 1 wherein the nematode is Heterorhabditis bacteriophora hybrid strain Hb(NJ).
3. A process for reducing the population of soil-inhabiting white grubs or otherinsects comprising applying to a soil locus infested with said insects an insecticidal composition comprising an effective amount of an infective-stage of an insecticidal entomopathogenic nematode having insecticidalproperties of Heterorhabditis bacteriophora hybrid strain Hb(NJ)) or variants or mutants thereof.
4. A process of Claim 3 wherein the nematodes are the infective-stage of Heterorhabditis bacteriophora hybrid strain Hb(NJ) nematodes.
5. A nematode of Heterorhabditis bacteriophora hybrid strain HB (NJ) capable in the juvenile infective-stage of reducing the population of one or more soil-inhabiting insects when applied to the infested soil locus.
6. A juvenile infective-stage of the nematode of Claim 5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38243095A | 1995-02-02 | 1995-02-02 | |
| US08/382,430 | 1995-02-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2211359A1 true CA2211359A1 (en) | 1996-08-08 |
Family
ID=23508911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002211359A Abandoned CA2211359A1 (en) | 1995-02-02 | 1996-01-25 | Heterorhabditis bacteriophora insecticide compositions and related processes |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0807167A4 (en) |
| AU (1) | AU5028196A (en) |
| CA (1) | CA2211359A1 (en) |
| WO (1) | WO1996023871A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2171131B1 (en) * | 2000-11-10 | 2003-12-16 | Idebio S L | QUITOSANE BASED BIOLOGICAL PESTICIDE |
| KR100450902B1 (en) * | 2001-12-14 | 2004-10-01 | 삼성에버랜드 주식회사 | Korean entomopathogenic nematode, heterorhabditis sp. gsn2 and control method for insect |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU6875691A (en) * | 1989-11-03 | 1991-05-31 | Rutgers, The State University Of New Jersey | Insecticide compositions, processes and devices |
-
1996
- 1996-01-25 EP EP96907120A patent/EP0807167A4/en not_active Withdrawn
- 1996-01-25 WO PCT/US1996/002603 patent/WO1996023871A1/en not_active Application Discontinuation
- 1996-01-25 CA CA002211359A patent/CA2211359A1/en not_active Abandoned
- 1996-01-25 AU AU50281/96A patent/AU5028196A/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0807167A1 (en) | 1997-11-19 |
| WO1996023871A1 (en) | 1996-08-08 |
| EP0807167A4 (en) | 1999-02-10 |
| AU5028196A (en) | 1996-08-21 |
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