CA2211165A1 - Procedes de detection sensible de transcriptase inverse - Google Patents
Procedes de detection sensible de transcriptase inverseInfo
- Publication number
- CA2211165A1 CA2211165A1 CA002211165A CA2211165A CA2211165A1 CA 2211165 A1 CA2211165 A1 CA 2211165A1 CA 002211165 A CA002211165 A CA 002211165A CA 2211165 A CA2211165 A CA 2211165A CA 2211165 A1 CA2211165 A1 CA 2211165A1
- Authority
- CA
- Canada
- Prior art keywords
- reverse transcriptase
- dna
- biological sample
- methods
- synthesized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100034343 Integrase Human genes 0.000 title abstract 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000011896 sensitive detection Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 4
- 239000012472 biological sample Substances 0.000 abstract 4
- 108020001019 DNA Primers Proteins 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 241001430294 unidentified retrovirus Species 0.000 abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 102000053602 DNA Human genes 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000013615 primer Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne un procédé servant à détecter la présence d'un rétrovirus dans un spécimen biologique consistant en les étapes suivantes: a) mise en contact du spécimen biologique avec une matrice d'ARN et avec une amorce complémentaire d'ADN dans des conditions permettant l'hybridation de la matrice d'ARN et de l'amorce d'ADN, ainsi que la synthèse d'un brin d'ADN en tant qu'extension à partir de l'amorce d'ADN si une transcriptase inverse est présente dans le spécimen; b) amplification de l'ADN synthétisé; c) détection de l'amplification de l'ADN synthétisé, ladite amplification indiquant la présence d'une transcriptase inverse dans le spécimen biologique, ce qui indique la présence d'un rétrovirus dans ledit spécimen.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37985195A | 1995-01-27 | 1995-01-27 | |
| US08/379,851 | 1995-01-27 | ||
| PCT/US1996/001257 WO1996023076A1 (fr) | 1995-01-27 | 1996-01-26 | Procedes de detection sensible de transcriptase inverse |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2211165A1 true CA2211165A1 (fr) | 1996-08-01 |
| CA2211165C CA2211165C (fr) | 2006-10-24 |
Family
ID=37309488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002211165A Expired - Fee Related CA2211165C (fr) | 1995-01-27 | 1996-01-26 | Procedes de detection sensible de transcriptase inverse |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2211165C (fr) |
-
1996
- 1996-01-26 CA CA002211165A patent/CA2211165C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2211165C (fr) | 2006-10-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20160126 |