CA2205545A1 - Method and apparatus for detecting and identifying microbial colonies - Google Patents

Method and apparatus for detecting and identifying microbial colonies

Info

Publication number
CA2205545A1
CA2205545A1 CA 2205545 CA2205545A CA2205545A1 CA 2205545 A1 CA2205545 A1 CA 2205545A1 CA 2205545 CA2205545 CA 2205545 CA 2205545 A CA2205545 A CA 2205545A CA 2205545 A1 CA2205545 A1 CA 2205545A1
Authority
CA
Canada
Prior art keywords
colonies
image
growth medium
detecting
potential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2205545
Other languages
French (fr)
Other versions
CA2205545C (en
Inventor
Robert A. Braier
Scott D. Morgan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3M Co
Original Assignee
Minnesota Mining And Manufacturing Company
Robert A. Braier
Scott D. Morgan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/356,484 external-priority patent/US5694478A/en
Application filed by Minnesota Mining And Manufacturing Company, Robert A. Braier, Scott D. Morgan filed Critical Minnesota Mining And Manufacturing Company
Publication of CA2205545A1 publication Critical patent/CA2205545A1/en
Application granted granted Critical
Publication of CA2205545C publication Critical patent/CA2205545C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Landscapes

  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Image Processing (AREA)

Abstract

A method and system for detecting and identifying microbial colonies in an inoculated growth medium first acquires a first image of the inoculated growth medium (100). After a selected incubation time interval (110), a second image of the growth medium is acquired (100). A difference image is produced by taking the difference between corresponding pixels in the first and second images (112). Potential colonies are identified within the difference image by locating peak pixels having local maximum pixel intensities within the difference image and having decreasing gradients in a predetermined number of directions (118). The potential colonies are validated as actual colonies by eliminating those potential colonies that are noise spikes or noise around previously validated colonies (120).
CA 2205545 1994-12-15 1995-09-20 Method and apparatus for detecting and identifying microbial colonies Expired - Fee Related CA2205545C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/356,484 US5694478A (en) 1994-12-15 1994-12-15 Method and apparatus for detecting and identifying microbial colonies
US08/356484 1994-12-15
PCT/US1995/011866 WO1996018720A1 (en) 1994-12-15 1995-09-20 Method and apparatus for detecting and identifying microbial colonies

Publications (2)

Publication Number Publication Date
CA2205545A1 true CA2205545A1 (en) 1996-06-20
CA2205545C CA2205545C (en) 2008-01-29

Family

ID=29405750

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2205545 Expired - Fee Related CA2205545C (en) 1994-12-15 1995-09-20 Method and apparatus for detecting and identifying microbial colonies

Country Status (1)

Country Link
CA (1) CA2205545C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972224A (en) * 2020-07-20 2020-11-24 李绪臣 Mushroom toxicity field analysis system
CN114317675A (en) * 2022-01-06 2022-04-12 福州大学 Detection method and system for qualitatively and quantitatively detecting bacteria on different wound surfaces based on machine learning
CN114842791A (en) * 2021-02-02 2022-08-02 瀚宇彩晶股份有限公司 Display device and pixel repairing method thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3286732A2 (en) * 2015-04-23 2018-02-28 BD Kiestra B.V. Method and system for automatically counting microbial colonies

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111972224A (en) * 2020-07-20 2020-11-24 李绪臣 Mushroom toxicity field analysis system
CN114842791A (en) * 2021-02-02 2022-08-02 瀚宇彩晶股份有限公司 Display device and pixel repairing method thereof
CN114317675A (en) * 2022-01-06 2022-04-12 福州大学 Detection method and system for qualitatively and quantitatively detecting bacteria on different wound surfaces based on machine learning

Also Published As

Publication number Publication date
CA2205545C (en) 2008-01-29

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Legal Events

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EEER Examination request
MKLA Lapsed

Effective date: 20140922