CA2205545A1 - Method and apparatus for detecting and identifying microbial colonies - Google Patents
Method and apparatus for detecting and identifying microbial coloniesInfo
- Publication number
- CA2205545A1 CA2205545A1 CA 2205545 CA2205545A CA2205545A1 CA 2205545 A1 CA2205545 A1 CA 2205545A1 CA 2205545 CA2205545 CA 2205545 CA 2205545 A CA2205545 A CA 2205545A CA 2205545 A1 CA2205545 A1 CA 2205545A1
- Authority
- CA
- Canada
- Prior art keywords
- colonies
- image
- growth medium
- detecting
- potential
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Image Processing (AREA)
Abstract
A method and system for detecting and identifying microbial colonies in an inoculated growth medium first acquires a first image of the inoculated growth medium (100). After a selected incubation time interval (110), a second image of the growth medium is acquired (100). A difference image is produced by taking the difference between corresponding pixels in the first and second images (112). Potential colonies are identified within the difference image by locating peak pixels having local maximum pixel intensities within the difference image and having decreasing gradients in a predetermined number of directions (118). The potential colonies are validated as actual colonies by eliminating those potential colonies that are noise spikes or noise around previously validated colonies (120).
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/356,484 US5694478A (en) | 1994-12-15 | 1994-12-15 | Method and apparatus for detecting and identifying microbial colonies |
US08/356484 | 1994-12-15 | ||
PCT/US1995/011866 WO1996018720A1 (en) | 1994-12-15 | 1995-09-20 | Method and apparatus for detecting and identifying microbial colonies |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2205545A1 true CA2205545A1 (en) | 1996-06-20 |
CA2205545C CA2205545C (en) | 2008-01-29 |
Family
ID=29405750
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA 2205545 Expired - Fee Related CA2205545C (en) | 1994-12-15 | 1995-09-20 | Method and apparatus for detecting and identifying microbial colonies |
Country Status (1)
Country | Link |
---|---|
CA (1) | CA2205545C (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111972224A (en) * | 2020-07-20 | 2020-11-24 | 李绪臣 | Mushroom toxicity field analysis system |
CN114317675A (en) * | 2022-01-06 | 2022-04-12 | 福州大学 | Detection method and system for qualitatively and quantitatively detecting bacteria on different wound surfaces based on machine learning |
CN114842791A (en) * | 2021-02-02 | 2022-08-02 | 瀚宇彩晶股份有限公司 | Display device and pixel repairing method thereof |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3286732A2 (en) * | 2015-04-23 | 2018-02-28 | BD Kiestra B.V. | Method and system for automatically counting microbial colonies |
-
1995
- 1995-09-20 CA CA 2205545 patent/CA2205545C/en not_active Expired - Fee Related
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111972224A (en) * | 2020-07-20 | 2020-11-24 | 李绪臣 | Mushroom toxicity field analysis system |
CN114842791A (en) * | 2021-02-02 | 2022-08-02 | 瀚宇彩晶股份有限公司 | Display device and pixel repairing method thereof |
CN114317675A (en) * | 2022-01-06 | 2022-04-12 | 福州大学 | Detection method and system for qualitatively and quantitatively detecting bacteria on different wound surfaces based on machine learning |
Also Published As
Publication number | Publication date |
---|---|
CA2205545C (en) | 2008-01-29 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20140922 |