CA2190261C - Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene - Google Patents

Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene Download PDF

Info

Publication number
CA2190261C
CA2190261C CA 2190261 CA2190261A CA2190261C CA 2190261 C CA2190261 C CA 2190261C CA 2190261 CA2190261 CA 2190261 CA 2190261 A CA2190261 A CA 2190261A CA 2190261 C CA2190261 C CA 2190261C
Authority
CA
Canada
Prior art keywords
cells
ctls
antigen
vector
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 2190261
Other languages
English (en)
Other versions
CA2190261A1 (fr
Inventor
Claudio Bordignon
Fulvio Mavilio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to CA 2190261 priority Critical patent/CA2190261C/fr
Priority claimed from PCT/EP1994/001573 external-priority patent/WO1995031208A1/fr
Publication of CA2190261A1 publication Critical patent/CA2190261A1/fr
Application granted granted Critical
Publication of CA2190261C publication Critical patent/CA2190261C/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Procédé de production de lymphocytes T cytotoxiques spécifiques d'un antigène, par incubation, à l'aide de cet antigène, d'une préparation cellulaire renfermant des lymphocytes T, et par isolement desdits lymphocytes T cytotoxiques spécifiques de l'antigène. Le procédé est caractérisé par (i) l'activation de la prolifération des lymphocytes T cytotoxiques dans la préparation cellulaire par incubation à l'aide de l'antigène; (ii) le transfert sélectif d'un vecteur dans les lymphocytes T cytotoxiques en prolifération, ledit vecteur renfermant un gène marqueur permettant la séparation des cellules marquées et non marquées; et (iii) la séparation des lymphocytes T cytotoxiques spécifiques de l'antigène ainsi transfectés, en fonction dudit gène marqueur. Ledit procédé est utilisable dans l'immunomodulation.
CA 2190261 1994-05-16 1994-05-16 Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene Expired - Lifetime CA2190261C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA 2190261 CA2190261C (fr) 1994-05-16 1994-05-16 Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/EP1994/001573 WO1995031208A1 (fr) 1994-05-16 1994-05-16 Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene
CA 2190261 CA2190261C (fr) 1994-05-16 1994-05-16 Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene

Publications (2)

Publication Number Publication Date
CA2190261A1 CA2190261A1 (fr) 1995-11-23
CA2190261C true CA2190261C (fr) 2005-08-23

Family

ID=4159248

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2190261 Expired - Lifetime CA2190261C (fr) 1994-05-16 1994-05-16 Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene

Country Status (1)

Country Link
CA (1) CA2190261C (fr)

Also Published As

Publication number Publication date
CA2190261A1 (fr) 1995-11-23

Similar Documents

Publication Publication Date Title
CN107922925B (zh) 用于自然杀伤细胞扩增的方法
Marktel et al. Immunologic potential of donor lymphocytes expressing a suicide gene for early immune reconstitution after hematopoietic T-cell–depleted stem cell transplantation
Vera et al. Accelerated production of antigen-specific T cells for preclinical and clinical applications using gas-permeable rapid expansion cultureware (G-Rex)
Zheng et al. CD4+ and CD8+ regulatory T cells generated ex vivo with IL-2 and TGF-β suppress a stimulatory graft-versus-host disease with a lupus-like syndrome
Alici et al. Anti-myeloma activity of endogenous and adoptively transferred activated natural killer cells in experimental multiple myeloma model
Harada et al. Selective expansion of human natural killer cells from peripheral blood mononuclear cells by the cell line, HFWT
Glienke et al. GMP-compliant manufacturing of TRUCKs: CAR T cells targeting GD2 and releasing inducible IL-18
Regn et al. Ex vivo generation of cytotoxic T lymphocytes specific for one or two distinct viruses for the prophylaxis of patients receiving an allogeneic bone marrow transplant
Rooney et al. Adoptive immunotherapy of EBV-associated malignancies with EBV-specific cytotoxic T-cell lines
Peragine et al. Immunophenotypic and functional characterization of ex vivo expanded natural killer cells for clinical use in acute lymphoblastic leukemia patients
Garland et al. The use of Teflon cell culture bags to expand functionally active CD8+ cytotoxic T lymphocytes
EP0804210B1 (fr) Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene
Zhou et al. CD123 redirected multiple virus-specific T cells for acute myeloid leukemia
Takahashi et al. Dendritic cells generated from human blood in granulocyte macrophage-colony stimulating factor and interleukin-7
Paliard et al. Antigen specific and MHC nonrestricted cytotoxicity of T cell receptor alpha beta+ and gamma delta+ human T cell clones isolated in IL-4.
Mukherji et al. Cell-mediated amplification and down regulation of cytotoxic immune response against autologous human cancer.
CA2190261C (fr) Procede d'immunomodulation par transfert adoptif de lymphocytes t cytotoxiques specifiques d'un antigene
Dunnion et al. Human antigen‐presenting cell/tumour cell hybrids stimulate strong allogeneic responses and present tumour‐associated antigens to cytotoxic T cells in vitro
NZ267097A (en) Method of immunomodulation by means of adoptive transfer of antigen-specific cytotoxic t-cells
Ye et al. In vitro interactions between γδT cells, DC, and CD4+ T cells; implications for the immunotherapy of leukemia
Sportoletti et al. Interleukin-7–engineered mesenchymal cells: in vitro effects on naïve T-cell population
Wheatley III et al. Dendritic cells improve the generation of Epstein-Barr virus–specific cytotoxic T lymphocytes for the treatment of posttransplantation lymphoma
Petvises et al. Ex vivo generation of cytokine‐induced killer cells (CD3+ CD56+) from post‐stem cell transplant pediatric patients against autologous–Epstein–Barr virus–transformed lymphoblastoid cell lines
Carlens et al. Cytolytic T cell reactivity to Epstein-Barr virus is lost during in vitro T cell expansion
Andrews et al. Normal Immunologic Response to a Neoantigen, Bacteriophage ΦX-174, in Baboons With Long-Term Lymphohematopoietic Reconstitution From Highly Purified CD34+ Lin− Allogeneic Marrow Cells

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20140516